CN114767924A - Preparation method of mussel-like mucin hydrogel suitable for repairing damaged skin cells - Google Patents
Preparation method of mussel-like mucin hydrogel suitable for repairing damaged skin cells Download PDFInfo
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Abstract
The invention provides a preparation method of mussel-like mucin hydrogel suitable for repairing damaged skin cells, which comprises the following steps: (1) taking 5-8 wt% of mussel-like mucin solution, and adding sodium hyaluronate alkaline solution to obtain mussel-like mucin mixed pre-solution; (2) dripping alginate aqueous solution in water bath at 30-35 deg.C to obtain mussel-like mucin premix; (3) under magnetic stirring at 20-22 ℃, adding an enzymolysis snakehead collagen peptide liquid, and dropwise adding a sodium chloride aqueous solution to obtain a mussel-like mucin composition; (4) mixing chitosan water solution and Mesona polysaccharide at 5-8 deg.C to obtain chitosan polysaccharide composition solution; (5) under the condition of 35-45 ℃ water bath, the mussel-like mucin composition, triethanolamine and carbomer are added to obtain a finished product.
Description
Technical Field
The invention relates to the technical field of biological skin care materials, in particular to a preparation method of mussel-like mucin hydrogel suitable for repairing damaged skin cells.
Background
The repair of the damaged cells of the skin wound mainly comprises four stages of hemostasis stage, inflammation stage, cell proliferation stage and tissue remodeling stage. With the attention of people on the aspect of healing of superficial skin wound surfaces, various wound dressings such as films, hydrogels, sprays and the like appear at present.
In the process of repairing damaged skin cells, dressing is often peeled from a wound during dressing change, so that secondary damage to the wound surface is caused, and the hydrogel has good biocompatibility, is used as a flexible functional material, and is widely applied to repairing damaged cells of the skin wound surface. However, the existing composite hydrogel wound dressing has insufficient adhesion, is not beneficial to covering skin wound surfaces, has poor drug-loading slow-release performance, has low anti-inflammatory effect on local skin damaged cells, is not beneficial to healing and repairing of wound surfaces and the like, so that the protein hydrogel with stable slow-release effect, good adhesion to skin cells and remarkable anti-inflammatory effect is prepared, and is beneficial to promoting the wider application of the composite hydrogel in the skin wound surface cell repairing dressing.
Disclosure of Invention
In view of this, the present invention provides a method for preparing a mussel-like mucin hydrogel suitable for repairing damaged skin cells.
The technical scheme of the invention is realized as follows:
the invention provides a preparation method of mussel-like mucin hydrogel suitable for repairing damaged skin cells, which comprises the following steps:
(1) mussel-like mucin pre-mixing liquid:
dissolving mussel-like mucin in deionized water to obtain a mussel-like mucin solution with the concentration of 5-8 wt%, adding a sodium hyaluronate alkaline solution, and stirring uniformly at room temperature to obtain a mussel-like mucin pre-mixing solution;
(2) mussel-like mucin premix:
dropwise adding an alginate aqueous solution into the mixed pre-solution of the mussel-like mucin under the water bath condition of 30-35 ℃, and uniformly stirring at constant temperature to obtain a mussel-like mucin premix;
(3) mussel-like mucin composition
Adding a mussel-like mucin premix into an enzymolysis snakehead collagen peptide solution under the magnetic stirring state at the temperature of 20-22 ℃, and dropwise adding a sodium chloride aqueous solution, wherein the volume ratio of the mussel-like mucin premix to the enzymolysis snakehead collagen peptide solution to the sodium chloride aqueous solution is 3: 1-2: 4-6; the mass concentration of the sodium chloride solution is 3-3.5 g/L; obtaining a mussel-like mucin composition;
(4) chitosan polysaccharide carrier solution:
dissolving water-soluble chitosan in deionized water to prepare a chitosan water solution with the concentration of 10-12 wt%; mixing chitosan water solution and Mesona chinensis polysaccharide at 5-8 deg.C, and stirring to obtain chitosan polysaccharide composition solution;
(5) adding the mussel-like mucin composition, triethanolamine and carbomer into the chitosan polysaccharide composite solution under the condition of 35-45 ℃ water bath, and uniformly stirring to obtain the mussel-like mucin hydrogel.
Further, the dropping speed of the alginate water solution is 5-10 mL/min.
The sodium hyaluronate alkaline solution is prepared by dissolving sodium hyaluronate powder in NaOH alkaline aqueous solution to obtain sodium hyaluronate alkaline solution with pH of 10-11 and concentration of 3-6 w%.
Further, the volume ratio of the sodium hyaluronate alkali solution to the mussel-like mucin solution is as follows: 8-10:1-2.
Further explaining, the Mesona polysaccharide water is 80-100 deg.C hot water leaching liquor of Mesona chinensis Benth, filtering, concentrating and oven drying to obtain Mesona chinensis Benth; the mass ratio of the chitosan to the mesona chinensis benth polysaccharide is 2-4: 0.3.
further explaining, the alginate water solution is 20-22 wt% alginate water solution obtained by dissolving sodium alginate in water; the volume ratio of the mussel-like mucin before mixing to the alginate aqueous solution is as follows: 1:8-10.
Further, the enzymolysis snakehead collagen peptide liquid is obtained by adding protease into snakehead collagen for enzymolysis treatment and sterilization, wherein the molecular weight of the snakehead collagen peptide liquid is less than 1 kDa.
Further, the preparation method of the enzymolysis snakehead collagen peptide liquid comprises the following steps: adding snakehead collagen into acetic acid with the concentration of 0.05-0.1mol/L, wherein the feed-liquid ratio is 1: 30-40 mg/ml; adding 0.3-0.6 wt% of pepsin and 0.2-0.4 wt% of bromelain, carrying out enzymolysis at 10-12 ℃ for 2-3h, heating to 75 ℃, keeping the temperature, standing for 20-25min for enzyme inactivation, centrifuging, and separating by using an ultrafiltration membrane with the molecular weight less than 1kDa to obtain the collagen peptide liquid.
Further, the chitosan polysaccharide composition liquid, the mussel-like mucin composition, the triethanolamine and the carbomer are in the mass ratio of: (35-45):(6-8):(0.3-0.5):(0.05-0.1).
A mussel-like mucin hydrogel for repairing damaged skin cells prepared by the above method.
Compared with the prior art, the invention has the beneficial effects that:
(1) the mussel-like mucin is applied to the hydrogel preparation, and on the basis of obtaining the mussel-like mucin premix by adopting the combination of the sodium hyaluronate alkaline solution, the alginate aqueous solution and the mussel-like mucin, the mussel-like mucin premix, the enzymolysis snakehead collagen peptide liquid and the sodium chloride solution are combined under the low-temperature magnetic stirring effect, so that the mussel-like mucin composition with good stability and high adhesiveness is obtained.
(2) The invention firstly utilizes the premixing of the sodium hyaluronate alkaline solution and the mussel-like mucin, and then adopts the chitosan polysaccharide combined solution mixed by the chitosan aqueous solution and the mesona polysaccharide to carry out water bath mixing with the mussel-like mucin composition containing the enzymolysis snakehead collagen peptide, so as to obtain the mussel-like mucin hydrogel, which not only has good adhesion, fully covers the damaged cells of the skin wound surface and promotes the healing and the repair of the wound surface, but also is beneficial to improving the slow release effect of the protein hydrogel, releases the drug stably, is used for repairing the damaged skin barrier, ensures that the nursing period is longer and the effect is better.
Detailed Description
In order that the technical contents of the invention may be better understood, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
Example 1
The mussel-like mucin hydrogel suitable for repairing damaged skin cells comprises the following components in parts by weight: 35 parts of chitosan polysaccharide combined solution, 6 parts of mussel-like mucin composition, 0.3 part of triethanolamine and 0.05 part of carbomer.
The preparation method comprises the following steps:
(1) mussel-like mucin pre-mixing solution:
dissolving mussel-like mucin in deionized water to obtain a mussel-like mucin solution with the concentration of 5 wt%, adding a sodium hyaluronate alkaline solution with the pH of 10-11 and the concentration of 3 w%, and stirring uniformly at room temperature, wherein the volume ratio of the sodium hyaluronate alkaline solution to the mussel-like mucin solution is as follows: 8: 1; obtaining mixed pre-liquid of mussel-like mucin; wherein, the mussel-like mucin is prepared by recombining gene segments of the mussel mucin and utilizing a biological fermentation technology.
(2) Mussel-like mucin premix:
dropwise adding 20 wt% alginate aqueous solution into the mussel-like mucin mixture at a speed of 10mL/min under the condition of water bath at 32 ℃, and uniformly stirring at constant temperature, wherein the volume ratio of the mussel-like mucin mixture precursor to the alginate aqueous solution is as follows: 1: 10; obtaining a mussel-like mucin premix;
(3) mussel-like mucin composition:
adding the mussel-like mucin premix into an enzymolysis snakehead collagen peptide solution under the magnetic stirring state at the temperature of 20 ℃, and dropwise adding a sodium chloride aqueous solution at the speed of 35 mL/min; the volume ratio of the mussel-like mucin premix to the enzymolysis snakehead collagen peptide solution to the sodium chloride solution is 3: 1: 4; the mass concentration of the sodium chloride solution is 3.0 g/L; obtaining a mussel-like mucin composition;
wherein, enzymolysis of the snakehead collagen peptide liquid: adding snakehead collagen into acetic acid with the concentration of 0.05-0.1mol/L, wherein the feed-liquid ratio is 1: 30-40 mg/ml; adding 0.3-0.6 wt% of pepsin and 0.2-0.4 wt% of bromelain, carrying out enzymolysis for 2-3h at 10-12 ℃, heating to 75 ℃, keeping the temperature, standing for 20-25min for enzyme deactivation, centrifuging, and separating by using an ultrafiltration membrane with the molecular weight less than 1kDa to obtain a collagen peptide solution, which is the same as the following steps.
(4) Chitosan polysaccharide carrier solution:
dissolving water-soluble chitosan in deionized water to prepare a chitosan water solution with the concentration of 10 wt%; and uniformly mixing and stirring the chitosan aqueous solution and the mesona chinensis benth polysaccharide at the temperature of 5 ℃, wherein the mass ratio of the chitosan to the mesona chinensis benth polysaccharide is 2: 0.3; obtaining chitosan polysaccharide composite liquid;
(5) adding the mussel-like mucin composition, triethanolamine and carbomer into the chitosan polysaccharide composite solution under the condition of 38 ℃ water bath, and uniformly stirring to obtain the mussel-like mucin hydrogel.
Example 2
The mussel-like mucin hydrogel suitable for repairing damaged skin cells comprises the following components in parts by weight: 45 parts of chitosan polysaccharide combined solution, 8 parts of mussel-like mucin composition, 0.5 part of triethanolamine and 0.1 part of carbomer.
The preparation method comprises the following steps:
(1) mussel-like mucin pre-mixing solution:
dissolving mussel-like mucin in deionized water to obtain a mussel-like mucin solution with the concentration of 8 wt%, adding a sodium hyaluronate alkaline solution with the pH of 10-11 and the concentration of 6 w%, and stirring uniformly at room temperature, wherein the volume ratio of the sodium hyaluronate alkaline solution to the mussel-like mucin solution is as follows: 10: 2; obtaining mixed pre-solution of mussel-like mucin;
(2) mussel-like mucin premix:
dropwise adding 22 wt% alginate aqueous solution into the mussel-like mucin mixture at a speed of 5mL/min under a water bath condition at 33 ℃, and uniformly stirring at a constant temperature, wherein the volume ratio of the mussel-like mucin mixture precursor to the alginate aqueous solution is as follows: 1: 8; obtaining a mussel-like mucin premix;
(3) mussel-like mucin composition:
adding the mussel-like mucin premix into an enzymolysis snakehead collagen peptide solution under the magnetic stirring state at the temperature of 22 ℃, and dropwise adding a sodium chloride aqueous solution at the speed of 35 mL/min; the volume ratio of the mussel-like mucin premix to the enzymolysis snakehead collagen peptide solution to the sodium chloride solution is 3: 2: 6; the mass concentration of the sodium chloride solution is 3.5 g/L; obtaining a mussel-like mucin composition;
the enzymolysis snakehead collagen peptide liquid is obtained by adding protease into snakehead collagen to carry out enzymolysis treatment and sterilizing, wherein the molecular weight of the snakehead collagen peptide liquid is less than 1 kDa.
(4) Chitosan polysaccharide carrier solution:
dissolving water-soluble chitosan in deionized water to prepare a chitosan water solution with the concentration of 12 wt%; and at the temperature of 8 ℃, uniformly mixing and stirring the chitosan aqueous solution and the mesona chinensis benth polysaccharide, wherein the mass ratio of the chitosan to the mesona chinensis benth polysaccharide is 4: 0.3; obtaining chitosan polysaccharide composite liquid;
(5) and adding the mussel-like mucin composition, triethanolamine and carbomer into the chitosan polysaccharide combined solution under the condition of water bath at 40 ℃, and uniformly stirring to obtain the mussel-like mucin hydrogel.
Example 3
The mussel-like mucin hydrogel suitable for repairing damaged skin cells comprises the following components in parts by weight: 40 parts of chitosan polysaccharide combined solution, 7 parts of mussel-like mucin composition, 0.4 part of triethanolamine and 0.08 part of carbomer.
The preparation method comprises the following steps:
(1) mussel-like mucin pre-mixing solution:
dissolving mussel-like mucin in deionized water to prepare a mussel-like mucin solution with the concentration of 7 wt%, adding a sodium hyaluronate alkaline solution with the pH of 10-11 and the concentration of 5 w%, and stirring uniformly at room temperature, wherein the volume ratio of the sodium hyaluronate alkaline solution to the mussel-like mucin solution is as follows: 9: 1; obtaining mixed pre-solution of mussel-like mucin;
(2) mussel-like mucin premix:
dropwise adding 21 wt% alginate aqueous solution into the mussel-like mucin mixture at a speed of 8mL/min under a water bath condition at 33 ℃, and uniformly stirring at a constant temperature, wherein the volume ratio of the mussel-like mucin mixture to the alginate aqueous solution is as follows: 1: 9; obtaining a mussel-like mucin premix;
(3) mussel-like mucin composition:
adding the mussel-like mucin premix into an enzymolysis snakehead collagen peptide solution under the magnetic stirring state at 21 ℃, and dropwise adding a sodium chloride aqueous solution at 35 mL/min; the volume ratio of the mussel-like mucin premix to the enzymolyzed snakehead collagen peptide solution to the sodium chloride solution is 3:1.5: 5; the mass concentration of the sodium chloride solution is 3.3 g/L; obtaining a mussel-like mucin composition;
the enzymolysis snakehead collagen peptide liquid is obtained by adding protease into snakehead collagen for enzymolysis treatment and sterilization, wherein the molecular weight of the snakehead collagen peptide liquid is less than 1 kDa.
(4) Chitosan polysaccharide carrier solution:
dissolving water-soluble chitosan in deionized water to prepare 11 wt% chitosan water solution; and uniformly mixing and stirring the chitosan aqueous solution and the mesona chinensis benth polysaccharide at the temperature of 7 ℃, wherein the mass ratio of the chitosan to the mesona chinensis benth polysaccharide is 3: 0.3; obtaining chitosan polysaccharide composite liquid;
(5) adding the mussel-like mucin composition, triethanolamine and carbomer into the chitosan polysaccharide composite solution under the condition of 38 ℃ water bath, and uniformly stirring to obtain the mussel-like mucin hydrogel.
Comparative example 1
The mussel-like mucin hydrogel is suitable for repairing damaged skin cells, and the mussel-like mucin pre-mixing solution is not prepared.
The method specifically comprises the following steps: directly dripping a 7 wt% mussel-like mucin solution into a 21 wt% alginate aqueous solution at a concentration of 8mL/min under a water bath condition at 33 ℃ to obtain a mussel-like mucin premix which is used for preparing and obtaining a mussel-like mucin composition;
and, a chitosan-polysaccharide composite solution was obtained by mixing and stirring a chitosan aqueous solution, a sodium hyaluronate alkaline solution having a pH of 10 to 11 and a concentration of 5 w% and mesona chinensis benth polysaccharide in a proportion at 7 ℃, and the other steps were carried out under the same conditions as the preparation method of example 3.
Comparative example 2
A mussel-like mucin hydrogel suitable for repairing damaged skin cells is prepared by different methods.
The method comprises the following specific steps: in the steps 1 to 3, a mussel-like mucin solution with a concentration of 7 wt% is directly added into an alkaline sodium hyaluronate solution with a concentration of 5 w%, a 21 wt% alginate aqueous solution and an enzymolysis snakehead collagen peptide solution with a pH of 10 to 11 under a 33 ℃ water bath condition, after uniform mixing, 3.3g/L sodium chloride aqueous solution is dripped at a rate of 35mL/min to obtain a mussel-like mucin composition, and the conditions of the other steps are the preparation method of the example 3.
Example 4
The in vitro release effect of the mussel-like mucin-containing hydrogels prepared in the above examples and comparative examples, respectively, was determined.
(1) Weighing 8mg of mussel-like mucin hydrogel, adding into a 50mL centrifuge tube, immersing in 30mL of PBS solution, and placing into a constant temperature oscillator at 37 ℃ and 100 rpm. And 3mL of supernatant solution is taken out to replace the same amount of new PBS solution at different time of 6h, 12h, 18h, 24h and 30h respectively, and the concentration of the medicament is determined by adopting an ultraviolet-visible spectrophotometry.
(2) The release rate is the release amount of the mussel-like mucin composition in a certain time/total amount of the hydrogel mussel-like mucin composition multiplied by 100%. The results are shown in table 1 below:
TABLE 1
As can be seen from the above table, the mussel mucin hydrogel prepared in examples 1-3 of the invention has a stable release rate within 24 hours, and the release amount of the mussel-like mucin composition within 30 hours can reach about 70%, thereby indicating that the mussel mucin hydrogel of the invention has a good and stable slow release effect, is stable in drug release, is beneficial to repairing damaged skin barriers, has a longer nursing period, and improves the repairing effect of damaged cells.
As is clear from example 3 in comparison with comparative examples 1-2, comparative example 1 has a high release rate before 18h, a significantly reduced release rate after 24h, and a reduced release of the mussel-like mucin-like composition within 30 h; the comparative example 2 shows that the release rate is high within 12 hours, and the release amount is obviously reduced after 18 hours, which indicates that the mussel-like mucin pre-mixing solution is prepared by premixing the sodium hyaluronate alkaline solution and the mussel-like mucin, so that the sodium hyaluronate alkaline solution, the alginate aqueous solution and the mussel-like mucin are effectively combined to obtain the mussel-like mucin premix, and the mussel-like mucin premix, the enzymolytic snakehead collagen peptide solution and the sodium chloride solution are combined under the magnetic stirring action at low temperature, thereby being beneficial to improving the stable slow release property of the mussel mucin hydrogel.
Comparative example 3
The mussel-like mucin hydrogel is suitable for repairing damaged skin cells, and the mixing conditions of the mussel-like mucin premix and the enzymolysis snakehead collagen peptide liquid are different.
The method comprises the following specific steps: directly adding the enzymolysis snakehead collagen peptide liquid and the 3.3g/L sodium chloride aqueous solution into the mussel-like mucin premix at the room temperature of 27 ℃, uniformly stirring to obtain the mussel-like mucin composition, and carrying out the rest steps and conditions in the preparation method of the example 3.
Comparative example 4
Mussel-like mucin hydrogel suitable for repairing damaged skin cells, wherein in the mussel-like mucin composition, the mass concentration of sodium chloride solution is different; the sodium chloride solution mass concentration was 5g/L, and the other process conditions were the same as in the preparation of example 3.
Example 5
Cell adhesion was measured on the mussel-like mucin-containing hydrogels prepared in the above examples and comparative examples, respectively.
(1) Adopting 3 24-pore plates, wherein each pore plate is divided into 6 groups, taking a polylysine coated cover glass as a control group, and taking 100 mu L of the mussel-like mucin hydrogel of the embodiment 3 and the comparative examples 1-4; at 1X 10 cells3Per cm2Seeding HFF cells; placing at 37 deg.C and 5% CO2Culturing under a floating box;
(2) taking the pore plates after the co-culture for 6h, 12h and 18h respectively, discarding the culture medium, and washing for 1 time by using PBS buffer solution; adding 4% paraformaldehyde, fixing at room temperature for 20min, and removing paraformaldehyde; washing with PBS solution for 3 times, and discarding the PBS solution; adding DAPI staining solution, and dyeing at room temperature in dark for 8 min; sucking out the DAPI staining solution, washing with PBS solution for 3 times, removing suspended cells, placing under a fluorescence microscope, and counting the number of cells stained and attached to the hydrogel by the DAPI staining solution.
The results are shown in table 2 below:
TABLE 2
As can be seen from the above table, the adhesion of the mussel-like mucin hydrogel in example 3 to cells is significantly improved, and compared with comparative examples 1 to 4, the preparation method of the mussel-like mucin composition is changed in comparative example 2, and the cell adhesion after the mussel-like mucin composition is combined with the alginate aqueous solution and the chitosan polysaccharide composition is significantly reduced, which indicates that the mussel-like mucin hydrogel has more stable adhesion based on the premixing of the sodium hyaluronate alkaline solution and the mussel-like mucin and the mixing manner of the sodium hyaluronate aqueous solution and the enzymolysis snakehead collagen peptide solution; in addition, the adhesion of the comparative examples 3 to 4 is reduced compared with the comparative group, which indicates that the adhesion of the mussel-like mucin hydrogel is influenced when the mixing condition of the mussel-like mucin premix and the enzymolysis snakehead collagen peptide liquid is controlled.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A preparation method of mussel-like mucin hydrogel suitable for repairing damaged skin cells is characterized by comprising the following steps: the method comprises the following steps:
(1) mussel-like mucin pre-mixing liquid:
dissolving mussel-like mucin in deionized water to obtain 5-8 wt% mussel-like mucin solution, adding sodium hyaluronate alkaline solution, and stirring at room temperature to obtain mussel-like mucin mixed solution;
(2) mussel-like mucin premix:
dropwise adding an alginate aqueous solution into the mixed pre-solution of the mussel-like mucin under the water bath condition of 30-35 ℃, and uniformly stirring at constant temperature to obtain a mussel-like mucin premix;
(3) mussel-like mucin composition
Adding a mussel-like mucin premix into an enzymolysis snakehead collagen peptide solution under the magnetic stirring state at the temperature of 20-22 ℃, and dropwise adding a sodium chloride aqueous solution, wherein the volume ratio of the mussel-like mucin premix to the enzymolysis snakehead collagen peptide solution to the sodium chloride aqueous solution is 3: 1-2: 4-6; the mass concentration of the sodium chloride solution is 3-3.5 g/L; obtaining a mussel-like mucin composition;
(4) chitosan polysaccharide carrier solution:
dissolving water-soluble chitosan in deionized water to obtain 10-12 wt% chitosan water solution; mixing chitosan water solution and Mesona chinensis Benth polysaccharide at 5-8 deg.C, and stirring to obtain chitosan polysaccharide composition solution;
(5) adding the mussel-like mucin composition, triethanolamine and carbomer into the chitosan polysaccharide combined solution at 35-45 deg.C in water bath, and stirring to obtain mussel-like mucin hydrogel.
2. A method of preparing a mussel-like mucin-hydrogel suitable for use in the repair of damaged skin cells according to claim 1, wherein: the dropping speed of the alginate water solution is 5-10 mL/min.
3. A method of preparing a mussel-like mucin hydrogel suitable for use in the repair of damaged skin cells according to claim 1, wherein: the sodium hyaluronate alkaline solution is prepared by dissolving sodium hyaluronate powder in NaOH alkaline aqueous solution to obtain sodium hyaluronate alkaline solution with pH of 10-11 and concentration of 3-6 w%.
4. A method of preparing a mussel-like mucin hydrogel suitable for use in the repair of damaged skin cells according to claim 3, wherein: the volume ratio of the sodium hyaluronate alkali solution to the mussel-like mucin solution is as follows: 8-10:1-2.
5. A method of preparing a mussel-like mucin hydrogel suitable for use in the repair of damaged skin cells according to claim 1, wherein: the Mesona polysaccharide water is 80-100 deg.C hot water leaching liquor of Mesona chinensis Benth, filtering, concentrating and oven drying to obtain Mesona chinensis Benth; the mass ratio of the chitosan to the mesona polysaccharide is 2-4: 0.3.
6. a method of preparing a mussel-like mucin hydrogel suitable for use in the repair of damaged skin cells according to claim 1, wherein: the alginate water solution is 20-22 wt% alginate water solution prepared by dissolving sodium alginate in water; the volume ratio of the mussel-like mucin mixed pre-solution to the alginate aqueous solution is as follows: 1:8-10.
7. A method of preparing a mussel-like mucin-hydrogel suitable for use in the repair of damaged skin cells according to claim 1, wherein: the enzymolysis snakehead collagen peptide liquid is obtained by adding protease into snakehead collagen for enzymolysis treatment and sterilization, wherein the molecular weight of the snakehead collagen peptide liquid is less than 1 kDa.
8. A method of preparing a mussel-like mucin-hydrogel suitable for use in the repair of damaged skin cells according to claim 1, wherein: the preparation method of the enzymolysis snakehead collagen peptide liquid comprises the following steps: adding snakehead collagen into acetic acid with the concentration of 0.05-0.1mol/L, wherein the feed-liquid ratio is 1: 30-40 mg/ml; adding 0.3-0.6 wt% of pepsin and 0.2-0.4 wt% of bromelain, carrying out enzymolysis at 10-12 ℃ for 2-3h, heating to 75 ℃, keeping the temperature, standing for 20-25min for enzyme inactivation, centrifuging, and separating by using an ultrafiltration membrane with the molecular weight less than 1kDa to obtain the collagen peptide liquid.
9. A method of preparing a mussel-like mucin-hydrogel suitable for use in the repair of damaged skin cells according to claim 1, wherein: the chitosan polysaccharide combined solution, the mussel-like mucin composition, the triethanolamine and the carbomer are in the mass ratio of: (35-45):(6-8):(0.3-0.5):(0.05-0.1).
10. A mussel-like mucin hydrogel prepared by the method of any one of claims 1 to 9 for use in the repair of damaged skin cells.
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