CN114766602B - Preparation and performance detection method of coated tryptophan pellet feed - Google Patents
Preparation and performance detection method of coated tryptophan pellet feed Download PDFInfo
- Publication number
- CN114766602B CN114766602B CN202210383926.8A CN202210383926A CN114766602B CN 114766602 B CN114766602 B CN 114766602B CN 202210383926 A CN202210383926 A CN 202210383926A CN 114766602 B CN114766602 B CN 114766602B
- Authority
- CN
- China
- Prior art keywords
- tryptophan
- coated
- feed
- pellet
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 title claims abstract description 94
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 title claims abstract description 84
- 239000008188 pellet Substances 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 238000001514 detection method Methods 0.000 title abstract description 12
- 239000011248 coating agent Substances 0.000 claims abstract description 26
- 238000000576 coating method Methods 0.000 claims abstract description 25
- 239000000463 material Substances 0.000 claims abstract description 24
- 239000011162 core material Substances 0.000 claims abstract description 15
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 150000004671 saturated fatty acids Chemical class 0.000 claims abstract description 9
- 239000002245 particle Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 10
- 238000005243 fluidization Methods 0.000 claims description 6
- 238000005422 blasting Methods 0.000 claims description 5
- 238000002844 melting Methods 0.000 claims description 5
- 230000008018 melting Effects 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 24
- 210000004767 rumen Anatomy 0.000 abstract description 22
- 241001465754 Metazoa Species 0.000 abstract description 21
- 238000000034 method Methods 0.000 abstract description 14
- 239000004677 Nylon Substances 0.000 abstract description 11
- 229920001778 nylon Polymers 0.000 abstract description 11
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 7
- 238000000338 in vitro Methods 0.000 abstract description 7
- 230000015556 catabolic process Effects 0.000 abstract description 6
- 238000006731 degradation reaction Methods 0.000 abstract description 6
- 238000001727 in vivo Methods 0.000 abstract description 6
- 239000007853 buffer solution Substances 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 210000003165 abomasum Anatomy 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 229960004799 tryptophan Drugs 0.000 description 80
- 241000283690 Bos taurus Species 0.000 description 8
- 239000000872 buffer Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 7
- 229940024606 amino acid Drugs 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 241000282849 Ruminantia Species 0.000 description 5
- 210000001198 duodenum Anatomy 0.000 description 5
- 235000021050 feed intake Nutrition 0.000 description 5
- 235000013336 milk Nutrition 0.000 description 5
- 239000008267 milk Substances 0.000 description 5
- 210000004080 milk Anatomy 0.000 description 5
- 210000000813 small intestine Anatomy 0.000 description 5
- 235000014113 dietary fatty acids Nutrition 0.000 description 4
- 239000003797 essential amino acid Substances 0.000 description 4
- 235000020776 essential amino acid Nutrition 0.000 description 4
- 229930195729 fatty acid Natural products 0.000 description 4
- 239000000194 fatty acid Substances 0.000 description 4
- 150000004665 fatty acids Chemical class 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- QZAYGJVTTNCVMB-UHFFFAOYSA-N serotonin Chemical compound C1=C(O)C=C2C(CCN)=CNC2=C1 QZAYGJVTTNCVMB-UHFFFAOYSA-N 0.000 description 4
- 238000010998 test method Methods 0.000 description 4
- 230000012010 growth Effects 0.000 description 3
- 239000012798 spherical particle Substances 0.000 description 3
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 2
- 206010016717 Fistula Diseases 0.000 description 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 241000219000 Populus Species 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003674 animal food additive Substances 0.000 description 2
- 239000008139 complexing agent Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000002183 duodenal effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000003890 fistula Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229950006238 nadide Drugs 0.000 description 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 206010053759 Growth retardation Diseases 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 description 1
- XJLXINKUBYWONI-NNYOXOHSSA-O NADP(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-O 0.000 description 1
- 241000283903 Ovis aries Species 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000002180 anti-stress Effects 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 230000035611 feeding Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 229940075507 glyceryl monostearate Drugs 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000020905 low-protein-diet Nutrition 0.000 description 1
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 description 1
- 229960003987 melatonin Drugs 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
- B01J13/04—Making microcapsules or microballoons by physical processes, e.g. drying, spraying
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2/00—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic
- B01J2/003—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic followed by coating of the granules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0078—Testing material properties on manufactured objects
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Dispersion Chemistry (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
A preparation method of pellet feed and a performance detection method of pellet feed belong to the technical field of feed processing in animal husbandry, and the preparation method comprises the steps of preparing a core material, preparing a wall material, and mixing and coating the core material and the wall material according to a weight ratio to obtain coated tryptophan pellet feed. The performance detection method is a method combining in-vitro simulated digestive tract test and in-vivo nylon bag degradation test. By taking saturated fatty acid as a coating material, tryptophan is physically coated, and by detection, the function and the effect of a tryptophan coated sample are more stable than those of uncoated samples in buffer solutions with different pH values, so that the use effect is better, the utilization of tryptophan by animal organisms is promoted, and when the coated feed enters abomasum and intestinal tracts through rumen, the coated wall material can be effectively decomposed, the function of tryptophan is fully exerted, and the production performance is improved.
Description
Technical Field
The invention belongs to the technical field of animal husbandry feed processing, relates to a method for preparing pellet feed and detecting performance of pellet feed, and particularly relates to a method for preparing tryptophan pellet feed by using a coating technology and detecting performance of the coated feed.
Background
Amino acids include essential amino acids and non-essential amino acids. Essential amino acids include leucine, isoleucine, tryptophan, lysine, methionine, phenylalanine, valine and threonine. Tryptophan is one of eight essential amino acids necessary for human and animals, it cannot be synthesized by the body itself, and its metabolites, 5-hydroxytryptamine, melatonin, NAD (nicotinamide adenine dinucleotide), NADP and nicotinic acid, etc., are also involved in various regulation of animal feeding, growth, reproduction, immunity, anti-stress, etc., and are called important precursor substances. Tryptophan is also an important component of the constituent protein, can influence animal behaviors, reduce stress response, can influence fat metabolism in an animal body, reduce fat content in animal livers, and can increase animal feed intake and improve production performance and feed conversion rate. At present, tryptophan is not only applied to the medicine industry and the food industry, but also gradually applied to the feed additive industry.
Han Xufeng and the like take Beijing ducks of 1-14 days old as study objects, and the study finds that: the daily feed intake and daily gain of the Beijing duck are obviously influenced by the addition level of the L-tryptophan (P is smaller than 0.05), the daily feed intake is in a trend of rising first and then reducing along with the increase of the tryptophan level, and the effect of adding 0.202% of the L-tryptophan is optimal. He Jiang with the young poplar green-shell layer as a research object, tryptophan with a proper level is added into the diet deficient in tryptophan, so that the laying rate of the young poplar green-shell layer can be improved, and the feed-egg ratio can be reduced. Wang Rongfa et al believe that the test pigs exhibit optimal growth performance at a digestible L-tryptophan content of 0.146% in low protein diets. From this, it can be seen that increasing the tryptophan addition can increase the feed intake of poultry and pigs. Studies in ruminants have found that tryptophan is the third limiting amino acid in growing lambs by the Nolte (2008) study. Tryptophan deficiency can lead to animal growth retardation, low feed intake, poor stress resistance, coarse and messy fur, etc. A large number of researches also show that the tryptophan supplement increases the tryptophan supply of small intestine, improves the tryptophan deficiency of cows in the later period of pregnancy, improves the milk yield, improves the content of 5-hydroxytryptamine in the brain, and promotes the secretion of hormones such as PRL, GH and the like, thereby promoting the secretion of milk.
At present, the coating technology is a technology for protecting active substances in feed additives, ensuring consistency and high efficiency of products, and plays an important role in improving the stability of the products, covering bad smell, controlling the release of the products in digestive tracts, improving bioavailability and the like. The technology of coating amino acids is mainly focused on ruminants, because the rumen of ruminants has a powerful fermentation function, and amino acids are degraded by rumen microorganisms after entering the rumen, so that the amino acids cannot be effectively supplied to the body. To reduce losses, the amino acids are treated using coating techniques. However, when tryptophan is taken as a feed by animals, the tryptophan can act in the bodies of the animals quickly, because the tryptophan belongs to small-molecule nutrient substances, when the tryptophan is taken and used in a large amount, the absorption and utilization efficiency of the animals can be reduced, and the method of taking a small amount of feed for multiple times obviously increases the workload of animal feeding. When the tryptophan feed is coated, the coating material occupies larger specific weight of the product (the thicker the coating film is), the release rate is smaller, and when the coating film is too thick, the particle size diameter of the coated product is obviously increased, and the release rate of the product in the real stomach and the small intestine is reduced.
Therefore, the method for preparing the tryptophan by using the coating technology according to the tryptophan content required by the animal has the advantages of effectively slowing down the release speed of the tryptophan in the animal body, promoting the utilization of the tryptophan by the animal body and having important significance in improving the production performance. The selection of the coating material, the specific weight of the coating material in the product and the detection of the coating performance are tryptophan, which are technical problems to be solved in the coating technology.
Disclosure of Invention
The invention aims to solve the technical problems in the prior tryptophan feed coating technology and the defects of the coated feed in the using process, and provides a preparation and performance detection method of the coated tryptophan pellet feed.
The technical scheme of the invention is as follows: a preparation method of coated tryptophan pellet feed, which is characterized by comprising the following steps:
(1) Preparing a core material;
(1-1) mixing tryptophan with water, and performing shot blasting treatment to obtain spherical pellet particles;
(1-2) sieving the pellet particles according to the particle size of the particles;
(1-3) drying the screened pellet particles to obtain pellet particles with the water content less than or equal to 10wt%;
(1-4) fluidization treatment is carried out on the dried pellet particles to obtain a required core material;
(2) Preparing a wall material;
(2-1) melting the saturated fatty acid;
(2-2) cooling the melted saturated fatty acid to obtain a required wall material;
(3) And mixing and coating the core material and the wall material according to the weight ratio to obtain the coated tryptophan pellet feed.
Further, the particle diameter in the step (1-2) has a particle diameter value of 1.5 to 8mm.
Further, the drying temperature of the drying treatment in the step (1-3) is 50-70 ℃ and the drying time is 50-80 min.
Further, in the step (1-4), the fluidization treatment is fluidization by a fluidized bed, wherein the air pressure of the fluidized bed is 0.012-0.024 MPa, the rotating speed is 90-100 r/min, the air inlet temperature is 35-45 ℃, and the air outlet temperature is 25-35 ℃.
Further, in the step (2-1), the saturated fatty acid is any one of C16-C18 fatty acid, glyceryl monostearate, hydrogenated fatty acid and fatty alcohol, and the melting temperature of the saturated fatty acid is 65-80 ℃.
Further, the cooling temperature of the saturated fatty acid in the step (2-2) is 50 to 55 ℃.
Further, in the step (3), the weight ratio of the core material to the wall material is 1:1-1:2.
A method for detecting the performance of coated tryptophan pellet feed is characterized by comprising the following steps: the detection method is a method combining in-vitro simulated digestive tract test and in-vivo nylon bag degradation test.
Further, the in vitro simulated digestive tract test method described in step (8) is as follows:
preparing buffer solutions with different pH values, taking test groups with different tryptophan mass percentages and uncoated tryptophan as control groups, respectively placing the test groups and the control groups at the bottoms of 50ml test tubes with plugs, adding 20ml buffer solution, and covering the test tube plugs; and (3) digestion and taking out in a constant-temperature water bath shaker at 39 ℃, flushing, filtering, fixing the volume of the filtrate, measuring the tryptophan content in the filtrate, and finally calculating the rumen bypass rate of tryptophan.
Further, the in-vivo nylon bag degradation test method in the step (8) is as follows:
preparing 3 milk cows with fistulae, taking coated tryptophan as a test group, taking uncoated tryptophan as a control group, putting nylon bags filled with tryptophan coating preparations into rumen fistulae of the milk cows in batches, taking out a net bag from rumen of each milk cow in a plurality of time periods, washing with clear water, drying in a constant-temperature oven until the weight is constant, weighing after cooling, and calculating the protection rate of tryptophan complexing agents in rumen.
The beneficial effects of the invention are as follows: the invention provides a preparation and performance detection method of coated tryptophan pellet feed, which comprises the following steps: preparing a core material, preparing a wall material, and mixing and coating the core material and the wall material according to the weight ratio to obtain the coated tryptophan pellet feed. The performance detection method comprises the following steps: in vitro simulated digestive tract test + in vivo nylon bag degradation test. By taking saturated fatty acid as a coating material, tryptophan is physically coated, and by detection, the function and the effect of a tryptophan coated sample are more stable than those of uncoated samples in buffer solutions with different pH values, so that the use effect is better, the utilization of tryptophan by animal organisms is promoted, and when the coated feed enters abomasum and intestinal tracts through rumen, the coated wall material can be effectively decomposed, the function of tryptophan is fully exerted, and the production performance is improved.
Drawings
FIG. 1 is a flow chart of the preparation of tryptophan coated particles according to the invention.
FIG. 2 is a flow chart of the preparation of in vitro simulated digestive tract reagents according to the invention.
Detailed Description
The present invention is further illustrated in the following table and detailed description, which are to be understood as being provided solely for the purposes of illustrating the invention and not as a definition of the limits of the invention, for which modifications may be made by those skilled in the art to which the invention pertains after reading the invention, which modifications are intended to fall within the scope of the appended claims.
Example 1
A preparation method of coated tryptophan pellet feed comprises the following steps:
step 1: core material preparation
30kg of L-tryptophan and 1kg of water were mixed and subjected to shot blasting treatment, and shot blasted to 1.5mm spherical particles.
Step 2: mixing and drying
Drying at 50deg.C for 80min until the water content is less than 10wt% to obtain dried tryptophan pellets.
Step 3: fluidization
Pouring the dried tryptophan pellets into a storage tank of a fluidized bed, and adjusting the air pressure of the fluidized bed to 0.01MPa, the rotating speed to 90r/min, the air inlet temperature to 35 ℃ and the air outlet temperature to 25 ℃.
Step 4: wall material preparation
After melting 30kg of C16-C18 fatty acid, it was cooled to 55 ℃.
Step 5: coating
And mixing the core material and the wall material in a weight ratio of 1:1 to obtain the coated tryptophan particles.
Example 2
Unlike example 1 above, a method for preparing coated tryptophan pellet feed includes the steps of:
step 1: core material preparation
30kg of L-tryptophan and 1kg of water were mixed and subjected to shot blasting treatment, and shot blasting was performed until 3mm spherical particles were obtained.
Step 2: screening is carried out
And removing spherical particles with the diameter smaller than 3 mm.
Step 3: mixing and drying
And (3) drying at 70 ℃ for 50min until the water content is less than or equal to 10wt% to obtain the dried tryptophan pellets.
Step 4: fluidization
Pouring the dried tryptophan pellets into a storage tank of a fluidized bed, and adjusting the air pressure of the fluidized bed to 0.024MPa, the rotating speed to 100r/min, the air inlet temperature to 45 ℃ and the air outlet temperature to 35 ℃.
Step 5: wall material preparation
After melting 60kg of hydrogenated fatty acid, it was cooled to 50 ℃.
Step 6: coating
And mixing the core material and the wall material in a weight ratio of 1:2 to obtain the coated tryptophan particles.
Example 3
The method for detecting the performance of the coated tryptophan pellet feed adopts an in-vitro simulated digestive tract test, and comprises the following specific operation methods:
step 1: test materials
A buffer with a pH value of 6.6 and a buffer with a pH value of 2.4, wherein the buffer with the pH value of 6.6 is used for simulating the rumen environment of ruminants, and the buffer with the pH value of 2.4 is used for simulating the duodenal environment of ruminants. Tryptophan samples A, B were coated with 30% tryptophan by mass and 45% tryptophan by mass, respectively, and tryptophan samples C were not coated.
Step 2: test method
Testing the stability in buffers with different pH values, taking uncoated tryptophan as a control group, weighing 1g of each sample A, B, C, placing at the bottom of a 50ml test tube with a plug, adding 20ml buffer, and covering the plug of the test tube; digesting for 2h, 4h, 8h, 12h and 24h in a constant temperature water bath shaker at 39 ℃; taking out, washing, filtering, fixing the volume of the filtrate, measuring the tryptophan content in the filtrate, and calculating the rumen bypass rate of tryptophan.
Analysis of results:
(1) Rumen bypass rates for each sample at various time points are shown in table 1.
TABLE 1 rumen bypass Rate (%)
(2) The small intestine release rates of each sample at different time points are shown in table 2.
TABLE 2 Small intestine Release Rate (%)
From the test data, the rumen bypass effect of each test article in the buffer with the pH value of 6.6 and the buffer with the pH value of 2.4 is as follows: sample B > sample a > sample C. The tryptophan coated samples were more stable than the uncoated samples, indicating better tryptophan coating. Uncoated sample C may be digested in the rumen and not reach the small intestine.
Example 4
The method for detecting the performance of the coated tryptophan pellet feed adopts an in-vivo nylon bag degradation test, and comprises the following specific operation methods:
step 1: test materials
3 fistula cows are selected as test animals, and the animals have good growth and health, and the variety, strain, age, sex, weight and the like should be consistent. Coating tryptophan samples, nylon bags: a nylon bag of 50X 30mm is produced by selecting 300 mesh polyester cloth. About 2g of the sample was weighed and put into a nylon bag, and the mass of the sample was recorded as m. The seal was again weighed to m1 and numbered.
Step 2: test method
3 fistula cows are selected in a pasture, and the animals grow well and are healthy, and the variety, strain, age, sex, weight and the like should be consistent. The nylon bags filled with about 2.0g tryptophan coating agent are put into rumen fistulae of cows in batches, one net bag is taken out of the rumen of each cow at each time point at 1.5, 3, 6, 12, 18 and 24 hours respectively, washed by clean water, dried to constant weight (8 hours) in a constant temperature oven at 65 ℃, cooled and weighed, and the release rate of tryptophan complexing agent in the rumen and duodenum is calculated, so that the stability of the coated tryptophan is detected.
Analysis of results:
the release rates of the coated tryptophan in the rumen and the duodenum are shown in table 3.
TABLE 3 coating tryptophan Release Rate in rumen and duodenum
| 1.5h | 3h | 6h | 12h | 18h | 24h | |
| Rumen (%) | 2 | 15.45 | 24.96 | 43.66 | 72.75 | 75.88 |
| Duodenum (%) | / | / | 84.66 | 86.43 | 90.29 | 95.66 |
From the experimental data, the stability of the coated tryptophan in the rumen decreases with increasing time. The release rate of the coated tryptophan in the duodenum increases with the increase of time, and the release rate at each time point is higher. The release rate can reach more than 84%, so that the coated tryptophan product has better digestibility in duodenal juice.
The test obtains the function and the effect of the coated tryptophan to be more stable through the detection method of the in vitro simulated digestive tract and in vivo nylon bag degradation test.
Claims (1)
1. A preparation method of coated tryptophan pellet feed, which is characterized by comprising the following steps:
(1) Preparing a core material;
(1-1) mixing tryptophan with water, and performing shot blasting treatment to obtain spherical pellet particles;
(1-2) sieving the pellet particles according to the particle size of 1.5-8 mm;
(1-3) drying the screened pellet particles to obtain pellet particles with the water content less than or equal to 10wt%;
(1-4) fluidization treatment is carried out on the dried pellet particles to obtain a required core material;
(2) Preparing a wall material;
(2-1) melting saturated fatty acid at 65-80 ℃;
(2-2) cooling the melted saturated fatty acid to 50-55 ℃ to obtain a required wall material;
(3) And mixing and coating the core material and the wall material according to the weight ratio of 1:1 to obtain the coated tryptophan pellet feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210383926.8A CN114766602B (en) | 2022-04-12 | 2022-04-12 | Preparation and performance detection method of coated tryptophan pellet feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210383926.8A CN114766602B (en) | 2022-04-12 | 2022-04-12 | Preparation and performance detection method of coated tryptophan pellet feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114766602A CN114766602A (en) | 2022-07-22 |
| CN114766602B true CN114766602B (en) | 2024-01-16 |
Family
ID=82429828
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210383926.8A Active CN114766602B (en) | 2022-04-12 | 2022-04-12 | Preparation and performance detection method of coated tryptophan pellet feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114766602B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116068134B (en) * | 2023-03-07 | 2023-06-13 | 中国农业科学院北京畜牧兽医研究所 | Method for evaluating slow release effect of coated urea feed |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111011618A (en) * | 2019-12-23 | 2020-04-17 | 辽宁亚禾营养科技有限责任公司 | Preparation method of rumen-protected tryptophan particles |
-
2022
- 2022-04-12 CN CN202210383926.8A patent/CN114766602B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111011618A (en) * | 2019-12-23 | 2020-04-17 | 辽宁亚禾营养科技有限责任公司 | Preparation method of rumen-protected tryptophan particles |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114766602A (en) | 2022-07-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107815429B (en) | Granulation-resistant lactobacillus preparation and preparation method thereof | |
| WO2018049657A1 (en) | Healthy nutritious feed for animals | |
| CN109362950B (en) | Rumen-protected choline chloride microcapsule and preparation method thereof | |
| JPH0479844A (en) | Feed additive for ruminant | |
| JP3448936B2 (en) | Methods for increasing milk production in ruminants | |
| CN105831433B (en) | Grape seed procyanidin additive and preparation method and application thereof | |
| KR20180029249A (en) | Protein-rich biomass of the Thraustochytrid, culture methods and uses | |
| CN114766602B (en) | Preparation and performance detection method of coated tryptophan pellet feed | |
| CN106798094A (en) | A kind of preparation method of rumen-protected glucose | |
| CN107988089B (en) | Preparation method of saccharomyces boulardii preparation and saccharomyces boulardii preparation | |
| CN107319211A (en) | A kind of hirudinaria manillensis young perfect compound feed and preparation method thereof | |
| CN110692821B (en) | Feed additive for improving rumen fermentation efficiency of cattle | |
| CN106071420A (en) | The preparation method of a kind of trace element chelated peptide and Litopenaeus vannamei breeding feed | |
| CN106998752A (en) | Increase the composition and the method using said composition of lactating ruminants fat yield | |
| KR20220005567A (en) | Composition for feed containing hexahydro-β-acid compound and application thereof | |
| CN108703264A (en) | A kind of feed and preparation method thereof reducing Roll road discharge of methane | |
| CN103652366B (en) | A kind of stabilization micro-capsule coating Mercaptamine and preparation method thereof | |
| CN111011618A (en) | Preparation method of rumen-protected tryptophan particles | |
| CA2176381A1 (en) | Method for raising dairy cattle with highly concentrated nutritious feed | |
| CN111480736A (en) | Rumen bypass choline chloride granules and preparation method thereof | |
| CN106804907A (en) | A kind of rumen bypass particle for ruminant feed | |
| CN114376082B (en) | Feed additive and preparation method and application thereof | |
| JP2023025243A (en) | Method for producing fodder for ruminant farm animals | |
| CN114698738B (en) | Rumen bypass schizochytrium limacinum powder and preparation method thereof | |
| CN111296693A (en) | Grass carp feed and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |