CN114766487A - Application of butenyl phthalide and butyl phthalide in preventing and treating crop southern blight - Google Patents
Application of butenyl phthalide and butyl phthalide in preventing and treating crop southern blight Download PDFInfo
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- CN114766487A CN114766487A CN202210351561.0A CN202210351561A CN114766487A CN 114766487 A CN114766487 A CN 114766487A CN 202210351561 A CN202210351561 A CN 202210351561A CN 114766487 A CN114766487 A CN 114766487A
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- phthalide
- butenyl
- southern blight
- butyl
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- HJXMNVQARNZTEE-UHFFFAOYSA-N Butylphthalide Chemical compound C1=CC=C2C(CCCC)OC(=O)C2=C1 HJXMNVQARNZTEE-UHFFFAOYSA-N 0.000 title claims abstract description 131
- 229950005197 butylphthalide Drugs 0.000 title claims abstract description 61
- RSKPJCDZAFMWHH-UHFFFAOYSA-N 3-but-1-enyl-3h-2-benzofuran-1-one Chemical compound C1=CC=C2C(C=CCC)OC(=O)C2=C1 RSKPJCDZAFMWHH-UHFFFAOYSA-N 0.000 title claims abstract description 57
- 241001530056 Athelia rolfsii Species 0.000 claims abstract description 26
- 239000003814 drug Substances 0.000 claims abstract description 25
- 239000000463 material Substances 0.000 claims abstract description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 51
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 239000008223 sterile water Substances 0.000 claims description 21
- 230000000694 effects Effects 0.000 claims description 20
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 17
- 229920000053 polysorbate 80 Polymers 0.000 claims description 17
- 230000035784 germination Effects 0.000 claims description 14
- 230000005764 inhibitory process Effects 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- 241001558929 Sclerotium <basidiomycota> Species 0.000 claims description 12
- 238000011282 treatment Methods 0.000 claims description 12
- 239000011259 mixed solution Substances 0.000 claims description 8
- 230000002265 prevention Effects 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 6
- 239000000375 suspending agent Substances 0.000 claims description 6
- 238000007865 diluting Methods 0.000 claims description 5
- 238000012795 verification Methods 0.000 claims description 5
- 230000001988 toxicity Effects 0.000 claims description 4
- 231100000419 toxicity Toxicity 0.000 claims description 4
- 239000000839 emulsion Substances 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims description 3
- 239000004530 micro-emulsion Substances 0.000 claims description 3
- 239000003094 microcapsule Substances 0.000 claims description 3
- 239000004563 wettable powder Substances 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims 1
- 239000012895 dilution Substances 0.000 claims 1
- 238000010790 dilution Methods 0.000 claims 1
- 230000003385 bacteriostatic effect Effects 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 15
- 241000196324 Embryophyta Species 0.000 description 13
- 244000105624 Arachis hypogaea Species 0.000 description 10
- 230000000844 anti-bacterial effect Effects 0.000 description 9
- 239000003899 bactericide agent Substances 0.000 description 7
- 229940079593 drug Drugs 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 235000020232 peanut Nutrition 0.000 description 7
- 239000002689 soil Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 229920002752 Konjac Polymers 0.000 description 6
- 229930182764 Polyoxin Natural products 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 6
- 235000010485 konjac Nutrition 0.000 description 6
- 235000017060 Arachis glabrata Nutrition 0.000 description 5
- 235000010777 Arachis hypogaea Nutrition 0.000 description 5
- 235000018262 Arachis monticola Nutrition 0.000 description 5
- 235000002566 Capsicum Nutrition 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- YEBIHIICWDDQOL-YBHNRIQQSA-N polyoxin Polymers O[C@@H]1[C@H](O)[C@@H](C(C=O)N)O[C@H]1N1C(=O)NC(=O)C(C(O)=O)=C1 YEBIHIICWDDQOL-YBHNRIQQSA-N 0.000 description 5
- 240000008574 Capsicum frutescens Species 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 230000003902 lesion Effects 0.000 description 4
- 244000247812 Amorphophallus rivieri Species 0.000 description 3
- 235000001206 Amorphophallus rivieri Nutrition 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- -1 aqua Substances 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 239000001390 capsicum minimum Substances 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 239000000252 konjac Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000001018 virulence Effects 0.000 description 3
- 241000758706 Piperaceae Species 0.000 description 2
- 241000576755 Sclerotia Species 0.000 description 2
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000223195 Fusarium graminearum Species 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241001330975 Magnaporthe oryzae Species 0.000 description 1
- 241000813090 Rhizoctonia solani Species 0.000 description 1
- 241000221662 Sclerotinia Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- KZNIFHPLKGYRTM-UHFFFAOYSA-N apigenin Chemical compound C1=CC(O)=CC=C1C1=CC(=O)C2=C(O)C=C(O)C=C2O1 KZNIFHPLKGYRTM-UHFFFAOYSA-N 0.000 description 1
- 229940117893 apigenin Drugs 0.000 description 1
- XADJWCRESPGUTB-UHFFFAOYSA-N apigenin Natural products C1=CC(O)=CC=C1C1=CC(=O)C2=CC(O)=C(O)C=C2O1 XADJWCRESPGUTB-UHFFFAOYSA-N 0.000 description 1
- 235000008714 apigenin Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000002872 contrast media Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- WNZQDUSMALZDQF-UHFFFAOYSA-N isobenzofuranone Natural products C1=CC=C2C(=O)OCC2=C1 WNZQDUSMALZDQF-UHFFFAOYSA-N 0.000 description 1
- 150000002611 lead compounds Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of butenyl phthalide and butylphthalide in preventing and treating crop southern blight, wherein a mixed medicament comprises the butenyl phthalide, the butylphthalide and auxiliary materials. According to the application of the butenyl phthalide and the butyl phthalide in preventing and treating the crop southern blight, the butenyl phthalide and the butyl phthalide have excellent bacteriostatic activity on the sclerotium rolfsii.
Description
Technical Field
The invention relates to the technical field of biological pesticides, in particular to application of butenyl phthalide and butyl phthalide in preventing and treating crop southern blight.
Background
Currently, research on the biological activity of butenyl phthalide and butyl phthalide against phytopathogenic fungi is relatively limited. Butylphthalide, also known as 3-butyl-1 (3H) -isobenzofuranone, apigenin, which is primarily responsible for the aroma and taste of celery. The research shows that the butenyl phthalide and the butyl phthalide have certain inhibitory activity on rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea, fusarium oxysporum and pyricularia oryzae. However, the biological activity of the butenyl phthalide and the butyl phthalide on the sclerotium rolfsii and the control effect on the crop southern blight have not been studied.
The sclerotium rolfsii has wide hosts, and can infect more than 500 plants, so that the infected plants turn yellow and wither, and roots of the plants turn brown and rot. Especially, southern blight of peanuts, peppers and konjak caused by sclerotium rolfsii has become an important factor restricting the safety production of the sclerotium rolfsii at present, and sclerotium generated by the pathogenic bacteria can survive in soil for 5-8 years and is difficult to prevent and treat. In 2020, agricultural rural halls in Henan province list peanut southern blight as a category II pest and disease damage directory in Henan province. More seriously, the sclerotinia sclerotiorum host range is still expanding. In the two years of 2019-.
At present, the measure for preventing and controlling the crop southern blight caused by sclerotium rolfsii is to use a bactericide, but the bactericide registered on the pesticide information network in China for preventing and controlling the crop southern blight is limited, and most of the bactericide is a chemical bactericide. The long-term use of the chemical bactericide not only easily causes drug resistance of the southern blight bacteria, but also causes the problems of pesticide residue, environmental pollution, harm to human health and the like. The search for a lead compound with high biological activity from natural products is one of the main ways of creating new pesticides, so that a novel, efficient and safe alternative medicament for preventing and treating crop southern blight is urgently needed to be searched.
Disclosure of Invention
The invention aims to provide application of butenyl phthalide and butylphthalide in preventing and treating crop southern blight, aims at solving the problems that the crop southern blight has limited prevention and treatment agents and is mostly chemical bactericide, and provides a novel, efficient and safe botanical bactericide for reasonable prevention and treatment.
In order to achieve the purpose, the invention provides application of butenyl phthalide and butylphthalide in preventing and treating crop southern blight, and the mixed medicament comprises the butenyl phthalide, the butylphthalide and auxiliary materials.
Preferably, the mixed medicament is used for preventing and treating crop southern blight caused by sclerotium rolfsii.
Preferably, the mixed medicament can be powder, wettable powder, soluble powder, granules, emulsion in water, microemulsion, aqua, suspending agent, dispersible oil suspending agent, microcapsule, missible oil and the like.
Preferably, the verification method of the butenyl phthalide and the butyl phthalide on the prevention and treatment of the crop southern blight is characterized by comprising the following steps:
s1, dissolving butenyl phthalide in 0.4mL of mixed solution of DMSO and Tween-80, adding sterile water to a constant volume of 20mL, and adding sterile water to sequentially dilute the solution to different concentrations in a gradient manner;
s2, dissolving butylphthalide in 0.4mL of mixed solution of DMSO and Tween-80, adding sterile water to a constant volume of 20mL, and sequentially diluting with sterile water in a gradient manner to different concentrations;
s3, determining the toxicity of butenyl phthalide and butyl phthalide on the growth of sclerotium rolfsii hypha by adopting a hypha growth rate inhibition method;
s4, verifying the inhibition effect of the butenyl phthalide and the butyl phthalide on sclerotium rolfsii sclerotium germination.
Preferably, the volume ratio of DMSO to Tween-80 is 1%.
Therefore, compared with the prior art, the application of the butenyl phthalide and the butyl phthalide in preventing and treating the crop southern blight has the following beneficial effects:
1. the butenyl phthalide sclerotium rolfsii has strong antibacterial activity, and the butenyl phthalide sclerotium rolfsii also has excellent inhibition effect on sclerotium germination;
2. the butylphthalide has excellent bacteriostatic activity on the sclerotium rolfsii, and the bacteriostatic activity is stronger along with the increase of the concentration of the butylphthalide;
3. the potted plant control effect test is consistent with the indoor toxicity test result, and the butyl phthalide and the butenyl phthalide have good application prospect in preventing and treating crop southern blight caused by sclerotium rolfsii.
The technical solution of the present invention is further described in detail by the following examples.
Detailed Description
The technical solution of the present invention is further illustrated by the following examples.
Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. The use of "first," "second," and similar terms in the present application do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
The application of the butenyl phthalide and the butyl phthalide in preventing and treating the southern blight of the crops, and the mixed medicament comprises the butenyl phthalide, the butyl phthalide and auxiliary materials.
The mixed medicament is used for preventing and treating the crop southern blight caused by sclerotinia rolfsii.
The mixed medicament can be powder, wettable powder, soluble powder, granules, emulsion in water, microemulsion, aqua, suspending agent, dispersible oil suspending agent, microcapsule, missible oil and the like.
The verification method of the butenyl phthalide and the butyl phthalide on the prevention and treatment of the crop southern blight is characterized by comprising the following steps of:
s1, dissolving butenyl phthalide in 0.4mL of mixed solution of DMSO and Tween-80, adding sterile water to a constant volume of 20mL, and adding sterile water to sequentially dilute the solution to different concentrations in a gradient manner;
s2, dissolving butylphthalide in 0.4mL of mixed solution of DMSO and Tween-80, adding sterile water to a constant volume of 20mL, and sequentially diluting with sterile water in a gradient manner to different concentrations;
s3, determining the toxicity of the butenyl phthalide and butyl phthalide on the growth of the sclerotium rolfsii hypha by adopting a hypha growth rate inhibition method;
s4, verifying the inhibition effect of the butenyl phthalide and the butyl phthalide on sclerotium rolfsii sclerotium germination.
The volume ratio of DMSO to Tween-80 is 1%.
A verification method of butenyl phthalide and butylphthalide on preventing and treating crop southern blight comprises the following steps:
1. the toxicity of the butenyl phthalide and butyl phthalide on the growth of the sclerotium rolfsii hypha is measured by adopting a hypha growth rate inhibition method:
s1, respectively dissolving 0.06g of butenyl phthalide (3-n-butyl phthalide CAS: 551-08-6) and butyl phthalide (n-butyl phthalide CAS: 6066-49-5) in 0.4mL of a mixed solution of DMSO and tween-80, adding 19.54mL of sterile water to 20mL of a mother solution prepared into 3000mg/L, wherein the volume ratio of the DMSO to the tween-80 is 1%;
s2, diluting the solution with sterile water in a gradient manner into seven liquid medicines with the concentration of 1500mg/L, 1000mg/L, 500mg/L, 250mg/L, 125mg/L, 62.5mg/L and 31.25 mg/L;
s3, adding 1mL of liquid medicine with different concentrations into 9mL of PDA culture medium respectively to prepare drug-containing culture medium with final concentrations of 150mg/L, 100mg/L, 50mg/L, 25mg/L, 12.5mg/L, 6.25mg/L and 3.125mg/L respectively;
s4, three replicates per concentration, against drug-free medium containing 0.05% DMSO and 0.05% tween-80;
s5, culturing in a light incubator at 25 ℃ in the dark, and measuring the diameter of each processed colony by a cross method after three days;
s6, calculating the inhibition rate of the growth of the hyphae of the whole sclerotium rolfsii aligned with the butenyl phthalide and the butyl phthalide, and obtaining a virulence regression equation, an effective medium concentration (EC50) and a concentration (EC90) with the inhibition rate of 90% according to a probability value analysis method;
the method for calculating the hypha growth inhibition rate comprises the following steps:
hypha growth inhibition rate ═ [ (control colony diameter-cake diameter) - (treated colony diameter-cake diameter) ]/(control colony diameter-cake diameter) × 100
Table 1: virulence of alignment of Butylphthalide and Butylphthalide to sclerotium rolfsii hyphae
| Treatment of | EC50(mg/L) | EC90(mg/L) | Coefficient of correlation r | Regression equation of virulence |
| Butylene phthalide | 14.0 | 64.4 | 0.9967 | y＝2.7849+1.9334x |
| Butylphthalide | 32.3 | 272.5 | 0.9860 | y＝2.9136+1.3830x |
| Polyoxins | 204.7 | 5763.7 | 0.9006 | y＝5.7643+1.0459x |
As shown in Table 1, the butenyl phthalide and the butyl phthalide have good inhibitory activity against sclerotium rolfsii. With the increase of the concentration of the butenyl phthalide and the butyl phthalide, the antibacterial activity of the compound is enhanced and is higher than that of a contrast medicament polyoxin.
2. Inhibition of sclerotium rolfsii sclerotium germination with respect to butenyl phthalide and butyl phthalide:
s1, dissolving 0.12g of butenyl phthalide (3-n-butyl phthalide CAS: 551-08-6) and butylphthalide (n-butyl phthalide CAS: 6066-49-5) in 0.4mL of mixed solution of DMSO and tween-80, adding 19.48mL of sterile water to reach a constant volume of 20mL, and preparing mother liquor of 6000mg/L, wherein the volume ratio of the DMSO to the tween-80 is 1%;
s2, diluting the solution with sterile water in a gradient manner to six liquid medicines with the concentration of 3000mg/L, 2500mg/L, 2000mg/L, 1500mg/L, 1000mg/L and 500 mg/L;
s3, adding 1mL of liquid medicine with different concentrations into 9mL of PDA culture medium respectively to obtain six kinds of drug-containing culture medium with final concentrations of 300mg/L, 250mg/L, 200mg/L, 150mg/L, 100mg/L and 50 mg/L;
s4, three replicates per concentration, against drug-free medium containing 0.05% DMSO and 0.05% tween-80;
s5, observing the germination condition of sclerotia after culturing for two days on a drug-containing culture medium, and regarding as germination when hyphae generated by the sclerotia is more than or equal to 2 mm;
the calculation method of the sclerotium germination inhibition rate comprises the following steps:
the sclerotium germination inhibition rate (control sclerotium germination number-treated sclerotium germination number)/(control sclerotium germination number) × 100.
Table 2: inhibitory Effect (%)% of Butylphthalide and Butylphthalide against sclerotium rolfsii germination
Table 2 shows that the butenyl phthalide and the butyl phthalide also have better inhibition effect on sclerotium germination of sclerotium rolfsii.
Example 1
The potted plant control effect of the butenyl phthalide and the butyl phthalide on the southern blight of the peanuts is as follows:
s1, selecting Yuhua 9326 as the peanut variety to be tested, placing the peanut seeds with plump grains and consistent sizes in a basin, adding one third of the seeds submerged in sterile water, and placing the seeds in an incubator for soaking for half a day;
s2, mixing matrix soil and vermiculite according to a volume ratio of 3:1, adding sterile water to adjust the water content of the soil to 70%, placing the soil in a pot, and sowing the germinated seeds with radicles facing downwards;
s3, artificially inoculating germs: after sowing for one week, selecting healthy peanut plants with consistent growth vigor and size, inoculating 1 fungus cake on the stem base of each peanut plant, and burying the peanut plants with soil;
s4, when hypha is observed to be generated on the stem base part of a plant and the surface of surrounding soil, spraying 10mL of liquid medicine of each plant on the root part of the plant and the surrounding soil by using 100mg/L, 200mg/L butenyl phthalide, butyl phthalide and 500mg/L contrast agent polyoxin liquid medicine respectively;
s5, using sterile water containing 0.1% DMSO and 0.1% Tween-80 as a control, treating 15 plants each, and repeating the steps for three times;
s6, after three days of drug application, identifying the disease condition according to the grading standard, and calculating the disease condition index and the prevention effect of different treatments;
table 3: potted plant control effect of butenyl phthalide and butylphthalide on peanut southern blight
Note: the data in the table are mean values ± standard error;
the different letters in the same column indicate significant differences at a P <0.05 level as tested by the least significant difference method.
The standard of the disease condition is identified as follows:
stage 0: no disease spots;
stage 1: the lesion spots are smaller than 1/3 stems;
and 3, level: the disease spots are larger than 2/3 stems, and a small amount of hypha is on the ground;
stage 5: the disease spots are larger than 2/3 stems, and a large amount of hypha is on the ground;
and 7, stage: the disease spot is larger than 2/3 stems, a large amount of hypha is on the ground, and wilting occurs on the ground;
and 9, stage: the plant withers;
the calculation method of the disease index comprises the following steps:
disease index ∑ (each stage of diseased plant × relative stage value)/(total plant number × 9) × 100
The calculation method of the control effect comprises the following steps:
the control effect is [ (control disease index-treatment disease index)/control disease index ] × 100%.
As can be seen from Table 3, the potting test and the indoor toxicity test result are consistent, the control effect of the butenyl phthalide and the butyl phthalide at the concentration of 200mg/L is obviously higher than that of the registered medicament of 500mg/L polyoxin, and the butenyl phthalide and the butyl phthalide have good effect on controlling peanut southern blight.
Example 2
The control effect of the butenyl phthalide and the butyl phthalide on the southern blight of the peppers and the konjak is as follows:
s1, selecting fresh hot pepper and konjak fruits with consistent size and quality, placing the fruits in a sterile plastic box with gauze and sterile water at the bottom, placing 9 fruits in each box, and inoculating two fungus cakes to each fruit;
s2, spraying 200mg/L and 100mg/L butenyl phthalide and butyl phthalide liquid medicines on the two crop fruits respectively when hypha or disease spots appear around the fruits;
s3, using 500mg/L polyoxin as a control agent, using sterile water containing 0.1% DMSO and 0.1% Tween-80 as a control, one treatment per plastic box, 9 replicates per treatment;
s4, measuring the diameter of the disease spot three days after the application, calculating the prevention and treatment effect,
the calculation formula of the control effect is as follows:
the control effect (%) (control group lesion diameter-treatment group lesion diameter)/control group lesion diameter × 100.
As can be seen from table 4, butenyl phthalide has an excellent therapeutic effect on capsicum and konjac southern blight. Under the dosage of 200mg/L, the control effect of the butenyl phthalide on the capsicum and konjac southern blight is obviously better than that of a control medicament polyoxin at 500 mg/L.
Table 4: control effect of butenyl phthalide and butyl phthalide on capsicum and konjac southern blight
Note: the data in the table are mean values ± standard error;
the different letters in the same column indicate significant differences at a P <0.05 level as tested by the least significant difference method.
Therefore, the application of the butenyl phthalide and the butyl phthalide with the structures in preventing and treating the crop southern blight, and the butenyl phthalide and the butyl phthalide have excellent bacteriostatic activity on the sclerotium rolfsii.
Finally, it should be noted that: the above embodiments are only intended to illustrate the technical solution of the present invention and not to limit the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the disclosed embodiments without departing from the spirit and scope of the present invention.
Claims (5)
1. The application of the butenyl phthalide and the butyl phthalide in preventing and treating the southern blight of crops is characterized in that: the mixed medicament comprises butenyl phthalide, butyl phthalide and auxiliary materials.
2. The use of the butenyl phthalide and butylphthalide as claimed in claim 1 for controlling crop southern blight, which comprises: the mixed medicament is used for preventing and treating crop southern blight caused by sclerotium rolfsii.
3. The use of the butenyl phthalide and butylphthalide as claimed in claim 1 for controlling crop southern blight, which comprises: the mixed medicament can be powder, wettable powder, soluble powder, granules, aqueous emulsion, microemulsion, aqueous solution, suspending agent, dispersible oil suspending agent, microcapsule, missible oil and the like.
4. The verification method of the butenyl phthalide and the butyl phthalide on preventing and treating the crop southern blight is characterized by comprising the following steps:
s1, dissolving butenyl phthalide in 0.4mL of mixed solution of DMSO and tween-80, adding sterile water to a constant volume of 20mL, and then adding sterile water to sequentially perform gradient dilution to different concentrations;
s2, dissolving butylphthalide in 0.4mL of mixed solution of DMSO and Tween-80, adding sterile water to a constant volume of 20mL, and sequentially diluting with sterile water in a gradient manner to different concentrations;
s3, determining the toxicity of the butenyl phthalide and butyl phthalide on the growth of the sclerotium rolfsii hypha by adopting a hypha growth rate inhibition method;
s4, verifying the inhibition effect of the butenyl phthalide and the butyl phthalide on sclerotium rolfsii sclerotium germination.
5. The verification method of the butenyl phthalide and butylphthalide on the prevention and treatment of the crop southern blight according to claim 4, characterized in that: the volume ratio of DMSO to Tween-80 is 1%.
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| CN115720897A (en) * | 2022-11-17 | 2023-03-03 | 河南农业大学 | Application of butenyl phthalide in preventing and treating Spodoptera frugiperda |
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| CN115720897B (en) * | 2022-11-17 | 2024-03-22 | 河南农业大学 | Application of butenyl phthalide in preventing and controlling spodoptera frugiperda |
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