CN114703300B - FABP4 gene molecular marker related to chicken carcass traits and application thereof - Google Patents
FABP4 gene molecular marker related to chicken carcass traits and application thereof Download PDFInfo
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Abstract
The invention discloses an FABP4 gene molecular marker related to chicken carcass traits and application thereof, and belongs to the field of gene detection. The invention obtains a new SNP molecular marker related to the chicken carcass traits through screening, and the molecular marker comprises the following mutation sites corresponding to the chicken FABP4 gene sequence: g.1549C > T, g.1604T > C, g.1631T > A, g.1786T > C, g.2036G > A, g.2043G > T, g.2090A > T, g.2727G > A, g.3283G > A. The chicken carcass traits can be screened at early stage by determining the genotype of the SNP mutation sites of the chicken, so that the production cost is saved, the genetic progress is accelerated, and the method is better applied to chicken genetic breeding. The molecular marker screened by the invention lays a foundation for breeding new strains with different chicken carcass traits, and has great economic application value.
Description
Technical Field
The invention relates to the field of gene detection, in particular to an FABP4 gene molecular marker related to chicken carcass traits and application thereof.
Background
Worldwide demand for meat is increasing, and how to produce more meat products using less resources becomes a serious issue. The carcass traits are important economic traits of the poultry breeding industry, can be visually presented to consumers, and are simple and direct embodiments of economic benefits of breeding enterprises.
Fatty acid binding protein 4 (FABP 4) is mainly expressed in macrophages and adipose tissues, regulates fatty acid storage and lipolysis, is a key regulatory gene of fat metabolism, and is also an important mediator of inflammation. The FABP4 can ubiquitinate and degrade the PPARg, if a mouse FABP4 gene is knocked out, the expression of the PPARg can be increased, and meanwhile, the mouse preadipocytes knocked out of the FABP4 gene show an obvious adipogenic effect, namely the FABP4 regulates the generation of fat by down-regulating the PPARg. Peripheral uptake of fatty acids by FABP4 through capillary endothelium is critical for systemic metabolism and may establish FABP4 as a potential new target for regulation of energy homeostasis. However, the FABP4 gene has no report related to poultry carcass traits at present.
Disclosure of Invention
The invention aims to provide an FABP4 gene molecular marker related to chicken carcass traits and application thereof, so as to solve the problems in the prior art, screen out a new molecular marker related to chicken carcass traits and lay a foundation for molecular marker-assisted selection of chicken strains with different carcass traits.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides an application of a reagent for detecting an SNP molecular marker in preparing a product for identifying the chicken carcass trait genotype, wherein the SNP molecular marker corresponds to a mutation site in a chicken FABP4 gene sequence and comprises the following components:
1549C > T, and the corresponding genotypes are TT, CT and CC;
1604T > C, the corresponding genotypes are TT, CT and CC;
g.1631T > A, its corresponding genotypes are TT, AT and AA;
g.1786T > C, the corresponding genotypes are TT, CT and CC;
2036G > A, and the corresponding genotypes are GG, AG and AA;
g.2043G > T, the corresponding genotypes are TT, GT and GG;
g.2090A > T, and the corresponding genotypes are TT, AT and AA;
2727G > A, corresponding to genotypes GG, AG and AA;
3283G > A, the corresponding genotypes are GG, AG and AA.
The invention also provides application of a specific primer pair for detecting the SNP molecular marker in identifying the genotype of the carcass trait of the chicken, wherein the SNP molecular marker corresponds to a mutation site in the FABP4 gene sequence of the chicken and comprises the following components:
1549C > T, its corresponding genotypes are TT, CT and CC;
1604T > C, the corresponding genotypes are TT, CT and CC;
g.1631T > A, the corresponding genotypes are TT, AT and AA;
g.1786T > C, its corresponding genotypes are TT, CT and CC;
g.2036G > A, the corresponding genotypes are GG, AG and AA;
g.2043G > T, the corresponding genotypes are TT, GT and GG;
2090A > T, and the corresponding genotypes are TT, AT and AA;
2727G > A, which corresponds to genotypes of GG, AG and AA;
3283G > A, corresponding genotypes are GG, AG and AA.
Preferably, the specific primer pair is as shown in SEQ ID NO:1-2 or the upstream and downstream primers shown in SEQ ID NO: 3-4.
Preferably, the carcass traits include live body weight, carcass weight, half bore weight, full bore weight, chest weight, leg weight, wing weight, foot weight, head weight, heart weight, liver weight, stomach weight, abdominal fat weight, shin length, shin girth and oblique body length.
The invention also provides application of a reagent for detecting the SNP molecular marker in preparing pure-strain chicken products with different chicken carcass traits, wherein the mutation sites of the SNP molecular marker corresponding to the chicken FABP4 gene sequence comprise:
1549C > T, its corresponding genotypes are TT, CT and CC;
1604T > C, the corresponding genotypes are TT, CT and CC;
g.1631T > A, the corresponding genotypes are TT, AT and AA;
g.1786T > C, its corresponding genotypes are TT, CT and CC;
g.2036G > A, the corresponding genotypes are GG, AG and AA;
g.2043G > T, the corresponding genotypes are TT, GT and GG;
g.2090A > T, and the corresponding genotypes are TT, AT and AA;
2727G > A, corresponding to genotypes GG, AG and AA;
3283G > A, the corresponding genotypes are GG, AG and AA;
the chicken carcass traits include live body weight, carcass weight, half bore weight, full bore weight, chest weight, leg weight, wing weight, foot weight, head weight, heart weight, liver weight, stomach weight, abdominal fat weight, shin length, shin girth, and body slant length.
The invention also provides application of a specific primer pair for detecting the SNP molecular marker in chicken genetic breeding, wherein the SNP molecular marker corresponds to a mutation site in a chicken FABP4 gene sequence and comprises the following components:
1549C > T, and the corresponding genotypes are TT, CT and CC;
1604T > C, the corresponding genotypes are TT, CT and CC;
g.1631T > A, its corresponding genotypes are TT, AT and AA;
g.1786T > C, its corresponding genotypes are TT, CT and CC;
g.2036G > A, the corresponding genotypes are GG, AG and AA;
g.2043G > T, the corresponding genotypes of which are TT, GT and GG;
2090A > T, and the corresponding genotypes are TT, AT and AA;
2727G > A, corresponding to genotypes GG, AG and AA;
3283G > A, the corresponding genotypes are GG, AG and AA;
breeding chicken strains with different carcass traits by using the specific primer pair; the specific primer pair is shown as SEQ ID NO:1-2 or the upstream and downstream primers shown in SEQ ID NO: 3-4.
The invention also provides a method for breeding chicken strains with different carcass traits, which comprises the steps of utilizing a specific primer pair to amplify a chicken FABP4 gene sequence by PCR, detecting the genotypes of SNP molecular markers g.1549C > T, g.1604T > C, g.1631T > A, g.1786T > C, g.2036G > A, g.2043G > T, g.2090A > T, g.2727G > A and g.3283G > A on an amplified product nucleotide sequence, and screening the chicken strains with different carcass traits according to the genotypes;
the specific primer pair is shown as SEQ ID NO:1-2 or the upstream and downstream primers shown in SEQ ID NO: 3-4.
The invention discloses the following technical effects:
the invention obtains new SNP molecular markers by screening, namely mutation sites g.1549C > T, g.1604T > C, g.1631T > A, g.1786T > C, g.2036G > A, g.2043G > T, g.2090A > T, g.2727G > A and g.3283G > A in a chicken FABP4 gene sequence, which are related to chicken carcass traits.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but rather as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
Example 1
1. Materials and methods
1.1 animal samples
560 yellow spotted-brown chickens (Guangzhou city Jiangfeng industry Co., ltd.) of 90 days old were selected. 2mL of neck blood was collected at slaughter and stored in a freezer at-80 ℃ for DNA sample extraction. Live body weight, shin length, shin circumference and body slant length are recorded before slaughter, and carcass traits such as carcass weight, half-bore weight and full-bore weight are recorded after slaughter.
1.2 Primary reagents
Blood sample DNA extraction kit (brand: OMEGA; cat # D3571-02; bei (Guangzhou) Biotechnology Co., ltd.), green Taq Mix (brand: nuozan; cat # P131-01; nanjing nuozan Biotechnology Co., ltd.), DL2000 Plus DNA Marker (brand: nuozan; cat # MD101-01; nanjing nuozan Biotechnology Co., ltd.).
1.3 Experimental methods
1.3.1 primer design
Primers were designed using NCBI's Primer-BLAST tool based on the sequence of the native chicken (gallinaceus) FABP4 Gene published by NCBI (National Center for Biotechnology Information) (Gene ID: 2167) and delivered to the Primer synthesis service provided by Ongzhou Ongchow Biotechnology Ltd. The information on the primer sequences is shown in Table 1.
TABLE 1PCR amplification primer sequences
The fragment sequence amplified by FABP4-1 is (SEQ ID NO: 5):
the base in the sequence with bold italic in black bottom corresponds to the mutation site: 2727, and 3283.
The fragment sequence amplified by FABP4-2 is (SEQ ID NO: 6):
bases in bold italics in the black matrix correspond in order from front to back to positions 1549, 1604, 1631, 1786, 2036, 2043 and 2090.
1.3.2 extraction of blood DNA
Blood DNA was extracted with reference to the instructions of the blood DNA extraction kit.
1.3.3 PCR amplification of the FABP4 Gene sequence
The PCR amplification system and the PCR running program of FABP4-1 and FABP4-2 are the same. The DNA extracted from the blood of 560 spotted-brown chickens was used as a template, and the following PCR amplification system was used: 3 mul of spotted-brown chicken genome template DNA, the concentration is 100-200 ng/mul; 1.2 mul of FABP4-1-F primer, 1.2 mul of FABP4-1-R primer, the concentration is 10nM;15 μ l of Green Taq Mix; 9.6. Mu.l of double distilled water, a total of 30. Mu.l. The PCR amplification procedure was: pre-denaturation at 95 ℃ for 5min; denaturation at 95 ℃ for 15s, annealing at 58 ℃ for 15s, and extension at 72 ℃ for 50s, for 32 cycles; completely extending for 5min at 72 ℃ at the end of the cycle; storing at 12 deg.C.
1.3.4SNPs identification and typing
Comparing the Sanger sequencing result of the PCR product with the downloaded FABP4 sequence by using DNASTAR software, determining potential SNP sites through a sequencing peak map, and counting and recording the SNPs data and the genotype of each sample.
The SPSS 26 is used for analyzing the genotype of SNPs sites and the carcass traits of corresponding individuals in a correlation mode.
2. Results
2.1FABP4 SNP site identification
A partial fragment of the spotted-brown chicken FABP4 gene is amplified by using PCR, wherein the partial fragment comprises three coding regions, partial introns and partial 3' -UTR. The DNA fragments obtained by amplification of the 560 samples were subjected to second-generation Sanger sequencing to obtain base sequences and sequencing peak maps of FABP4 fragments of the 560 samples. Comparing by using a Seqman program in DNASTAR software to identify all single nucleotide mutation sites, counting genotypes of the single nucleotide mutation sites, and identifying 9 SNP sites on the two segments of FABP4 fragments, wherein the SNP sites are respectively as follows: g.1549C > T, g.1604T > C, g.1631T > A, g.1786T > C, g.2036G > A, g.2043G > T, g.2090A > T, g.2727G > A and g.3283G > A.
2.2 Association analysis of SNP sites of FABP4 with carcass traits
We performed a correlation analysis of the genotype information of 9 SNP sites with 16 carcass traits (live weight, carcass weight, half-bore weight, full-bore weight, chest weight, leg weight, wing weight, foot weight, head weight, heart weight, liver weight, stomach weight, abdominal fat weight, shin length, shin girth, body slant length), and the results are listed in tables 2-4.
TABLE 2 correlation analysis results of SNPs with live body weight, carcass weight, half-bore weight, full-bore weight, breast muscle weight, and leg weight (Mean + -SEM)
TABLE 3 correlation of SNPs with wing weight, liver weight, abdominal fat weight, foot weight, head weight, stomach weight analysis results (Mean + -SEM)
TABLE 4 correlation analysis of SNPs with Heart weight, shank Length, shank circumference, body oblique Length (Mean + -SEM)
Statistical results of association analysis showed that all SNP sites had a very significant correlation with abdominal fat weight-average, and in addition, also had significant associations with weight and body slant length of living body, carcass, semi-bore, full bore, pectoral muscle, leg, wing, heart, liver, but where the g.3283g > a site did not have a significant association with weight of wing and liver. The characters of the TT genotype group of the g.1549C > T site are smaller than those of the CT and CC genotype groups, particularly in abdominal fat weight, the TT genotype individuals are obviously different from the CT and CC genotype individuals, and g.2090A > T also has the same trend. Each trait of the TT genotype group AT the g.1631t > a site is higher than that of the AT and AA genotype groups, but it is of interest that the abdominal fat weight of the TT genotype group is significantly higher than that of the AT and AA genotype groups. The average weight of TT genotype individuals of 1631T > A was at a higher level in the grouping of different genotype individuals at each SNP. g.1549c > T, g.1604t > C, g.1631t > a, g.2036g > a, g.2043g > T, g.2090a > T, g.2727g > a had significant associations with all carcass traits except foot weight, head weight, stomach weight, shin length and shin circumference, and were at very significant levels.
In addition, the top-weight trait was not associated with all SNPs. Only g.2036G > A, g.2043G > T and g.2090A > T sites have significant correlation with foot weight, and only g.2727G > A has significant correlation with shin circumference and stomach weight.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
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Claims (5)
1. The application of a reagent for detecting an SNP molecular marker in the preparation of a product for identifying the carcass trait genotype of spotted-brown chickens is characterized in that the SNP molecular marker corresponds to a mutation site in an FABP4 gene sequence of the spotted-brown chickens and comprises the following components:
1549C > T, its corresponding genotypes are TT, CT and CC;
1604T > C, the corresponding genotypes are TT, CT and CC;
g.1631T > A, the corresponding genotypes are TT, AT and AA;
g.1786T > C, its corresponding genotypes are TT, CT and CC;
g.2036G > A, the corresponding genotypes are GG, AG and AA;
g.2043G > T, the corresponding genotypes are TT, GT and GG;
2090A > T, and the corresponding genotypes are TT, AT and AA;
2727G > A, which corresponds to genotypes of GG, AG and AA;
3283G > A, the corresponding genotypes are GG, AG and AA;
the carcass traits include live body weight, carcass weight, half bore weight, full bore weight, breast muscle weight, leg weight, wing weight, foot weight, head weight, heart weight, liver weight, stomach weight, abdominal fat weight, shin length, shin girth, and oblique body length;
the g.1549c > T, g.1604t > C, g.1786t > C and g.1631t > a all have significant associations with abdominal fat weight, live body weight, carcass body weight, semi-bore weight, full bore weight, pectoral muscle weight, leg weight, wing weight, heart weight, liver weight and oblique body length;
the g.2036G > A, g.2043G > T and g.2090A > T all have significant correlation with abdominal fat weight, live weight, carcass weight, half-bore weight, full-bore weight, breast muscle weight, leg weight, wing weight, heart weight, liver weight, oblique body length and foot weight;
the g.2727G > A has a significant association with abdominal fat weight, live body weight, carcass weight, half bore weight, full bore weight, pectoral muscle weight, leg weight, wing weight, heart weight, liver weight, oblique length, tibioside and stomach weight;
the g.3283g > a had significant associations with abdominal fat weight, live body weight, carcass weight, half bore weight, full bore weight, pectoral muscle weight, leg weight, heart weight, and oblique body length.
2. The application of a specific primer pair for detecting an SNP molecular marker in identifying the carcass trait genotype of spotted-brown chickens is characterized in that the SNP molecular marker corresponds to a mutation site in an FABP4 gene sequence of the spotted-brown chickens and comprises the following steps:
1549C > T, its corresponding genotypes are TT, CT and CC;
1604T > C, the corresponding genotypes are TT, CT and CC;
g.1631T > A, its corresponding genotypes are TT, AT and AA;
g.1786T > C, the corresponding genotypes are TT, CT and CC;
g.2036G > A, the corresponding genotypes are GG, AG and AA;
g.2043G > T, the corresponding genotypes are TT, GT and GG;
2090A > T, and the corresponding genotypes are TT, AT and AA;
2727G > A, corresponding to genotypes GG, AG and AA;
3283G > A, the corresponding genotypes of which are GG, AG and AA;
the specific primer pair is shown as SEQ ID NO:1-2 and the upstream and downstream primers shown as SEQ ID NO:3-4, and upstream and downstream primers;
the carcass traits include live body weight, carcass weight, half bore weight, full bore weight, breast muscle weight, leg weight, wing weight, foot weight, head weight, heart weight, liver weight, stomach weight, abdominal fat weight, shin length, shin girth, and oblique body length;
the g.1549c > T, g.1604t > C, g.1786t > C and g.1631t > a all have significant associations with abdominal fat weight, live weight, carcass weight, half bore weight, full bore weight, pectoral muscle weight, leg weight, wing weight, heart weight, liver weight and oblique body length;
the g.2036G > A, g.2043G > T and g.2090A > T all have significant correlation with abdominal fat weight, live weight, carcass weight, half-bore weight, full-bore weight, breast muscle weight, leg weight, wing weight, heart weight, liver weight, oblique body length and foot weight;
the g.2727G > A has a significant association with abdominal fat weight, live body weight, carcass weight, half bore weight, full bore weight, pectoral muscle weight, leg weight, wing weight, heart weight, liver weight, oblique length, shin girth and stomach weight;
the g.3283g > a had significant associations with abdominal fat weight, live body weight, carcass weight, half-bore weight, full-bore weight, breast muscle weight, leg weight, heart weight, and oblique body length.
3. The application of a reagent for detecting the SNP molecular marker in the preparation of the spotted-brown chicken product for screening the pure strains of different spotted-brown chicken carcass traits is characterized in that the SNP molecular marker corresponding to the mutation sites in the spotted-brown chicken FABP4 gene sequence comprises the following steps:
1549C > T, its corresponding genotypes are TT, CT and CC;
1604T > C, the corresponding genotypes are TT, CT and CC;
g.1631T > A, its corresponding genotypes are TT, AT and AA;
g.1786T > C, the corresponding genotypes are TT, CT and CC;
2036G > A, and the corresponding genotypes are GG, AG and AA;
g.2043G > T, the corresponding genotypes are TT, GT and GG;
2090A > T, and the corresponding genotypes are TT, AT and AA;
2727G > A, corresponding to genotypes GG, AG and AA;
3283G > A, the corresponding genotypes of which are GG, AG and AA;
the carcass traits include live body weight, carcass weight, half bore weight, full bore weight, breast muscle weight, leg weight, wing weight, foot weight, head weight, heart weight, liver weight, stomach weight, abdominal fat weight, shin length, shin girth, and oblique body length;
the g.1549c > T, g.1604t > C, g.1786t > C and g.1631t > a all have significant associations with abdominal fat weight, live body weight, carcass body weight, semi-bore weight, full bore weight, pectoral muscle weight, leg weight, wing weight, heart weight, liver weight and oblique body length;
the g.2036G > A, g.2043G > T and g.2090A > T all have significant correlation with abdominal fat weight, live weight, carcass weight, half-bore weight, full-bore weight, breast muscle weight, leg weight, wing weight, heart weight, liver weight, oblique body length and foot weight;
the g.2727G > A has a significant association with abdominal fat weight, live body weight, carcass weight, half bore weight, full bore weight, pectoral muscle weight, leg weight, wing weight, heart weight, liver weight, oblique length, tibioside and stomach weight;
the g.3283g > a had significant associations with abdominal fat weight, live body weight, carcass weight, half bore weight, full bore weight, pectoral muscle weight, leg weight, heart weight, and oblique body length.
4. The application of a specific primer pair for detecting the SNP molecular marker in the genetic breeding of spotted-brown chickens is characterized in that the SNP molecular marker corresponding to the mutation site in the FABP4 gene sequence of the spotted-brown chickens comprises:
1549C > T, its corresponding genotypes are TT, CT and CC;
1604T > C, the corresponding genotypes are TT, CT and CC;
g.1631T > A, its corresponding genotypes are TT, AT and AA;
g.1786T > C, its corresponding genotypes are TT, CT and CC;
g.2036G > A, the corresponding genotypes are GG, AG and AA;
g.2043G > T, the corresponding genotypes are TT, GT and GG;
2090A > T, and the corresponding genotypes are TT, AT and AA;
2727G > A, which corresponds to genotypes of GG, AG and AA;
3283G > A, the corresponding genotypes of which are GG, AG and AA;
breeding the spotted-brown chicken strains with different carcass traits by utilizing the specific primer pair; the specific primer pair is shown as SEQ ID NO:1-2 and the upstream and downstream primers shown as SEQ ID NO:3-4, and upstream and downstream primers;
the carcass traits include live body weight, carcass weight, half bore weight, full bore weight, breast muscle weight, leg weight, wing weight, foot weight, head weight, heart weight, liver weight, stomach weight, abdominal fat weight, shin length, shin girth, and oblique body length;
the g.1549c > T, g.1604t > C, g.1786t > C and g.1631t > a all have significant associations with abdominal fat weight, live body weight, carcass body weight, semi-bore weight, full bore weight, pectoral muscle weight, leg weight, wing weight, heart weight, liver weight and oblique body length;
the g.2036G > A, g.2043G > T and g.2090A > T all have significant correlation with abdominal fat weight, live weight, carcass weight, half-bore weight, full-bore weight, breast muscle weight, leg weight, wing weight, heart weight, liver weight, oblique body length and foot weight;
the g.2727G > A has a significant association with abdominal fat weight, live body weight, carcass weight, half bore weight, full bore weight, pectoral muscle weight, leg weight, wing weight, heart weight, liver weight, oblique length, tibioside and stomach weight;
the g.3283g > a had significant associations with abdominal fat weight, live body weight, carcass weight, half-bore weight, full-bore weight, breast muscle weight, leg weight, heart weight, and oblique body length.
5. A method for breeding spotted-brown chicken strains with different carcass traits is characterized by comprising the steps of utilizing a specific primer pair to amplify a spotted-brown chicken FABP4 gene sequence by PCR, detecting the genotypes of SNP molecular markers g.1549C > T, g.1604T > C, g.1631T > A, g.1786T > C, g.2036G > A, g.2043G > T, g.2090A > T, g.2727G > A and g.3283G > A on an amplification product nucleotide sequence, and screening the spotted-brown chicken strains with different carcass traits according to the genotypes;
the specific primer pair is shown as SEQ ID NO:1-2 and the upstream and downstream primers shown as SEQ ID NO:3-4, and upstream and downstream primers;
the carcass traits include live body weight, carcass weight, half bore weight, full bore weight, breast muscle weight, leg weight, wing weight, foot weight, head weight, heart weight, liver weight, stomach weight, abdominal fat weight, shin length, shin girth, and oblique body length;
the g.1549c > T, g.1604t > C, g.1786t > C and g.1631t > a all have significant associations with abdominal fat weight, live weight, carcass weight, half bore weight, full bore weight, pectoral muscle weight, leg weight, wing weight, heart weight, liver weight and oblique body length;
the g.2036G > A, g.2043G > T and g.2090A > T all have significant correlation with abdominal fat weight, live weight, carcass weight, half-bore weight, full-bore weight, breast muscle weight, leg weight, wing weight, heart weight, liver weight, oblique body length and foot weight;
the g.2727G > A has a significant association with abdominal fat weight, live body weight, carcass weight, half bore weight, full bore weight, pectoral muscle weight, leg weight, wing weight, heart weight, liver weight, oblique length, tibioside and stomach weight;
the g.3283g > a had significant associations with abdominal fat weight, live body weight, carcass weight, half-bore weight, full-bore weight, breast muscle weight, leg weight, heart weight, and oblique body length.
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