CN114703099B - Mycobacterium JY-1 and application thereof in degrading organic pollutants - Google Patents

Mycobacterium JY-1 and application thereof in degrading organic pollutants Download PDF

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CN114703099B
CN114703099B CN202210359974.3A CN202210359974A CN114703099B CN 114703099 B CN114703099 B CN 114703099B CN 202210359974 A CN202210359974 A CN 202210359974A CN 114703099 B CN114703099 B CN 114703099B
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methylpentane
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organic pollutants
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CN114703099A (en
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陈东之
周佳宇
李钱
陈建孟
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Zhejiang University of Technology ZJUT
Zhejiang Ocean University ZJOU
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Zhejiang Ocean University ZJOU
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Abstract

The invention relates to a mycobacterium JY-1 strain and application thereof in degrading organic pollutants, belonging to the technical field of organic pollutant degradation, in particular to degradation of organic pollutants below C8. The mycobacterium of the present invention, designated as mycobacterium JY-1, has been deposited in China Center for Type Culture Collection (CCTCC) on the 2 nd month 25 th year 2022, with the deposit number of CCTCC No: m2022152, microorganism Classification designated as MycobacteriumMycobacteriumsp. The mycobacterium JY-1 can grow and reproduce by taking 2-methylpentane as a carbon source and an energy source, can degrade the 2-methylpentane efficiently, and can adapt to a wider temperature and pH range. In addition, the mycobacterium JY-1 can also take 3-methylpentane and n-hexane as carbon sources and energy sources to efficiently degrade the 3-methylpentane and the n-hexane.

Description

Mycobacterium JY-1 and application thereof in degrading organic pollutants
Technical Field
The invention relates to mycobacterium and application thereof in degrading organic pollutants, belonging to the technical field of degradation of organic pollutants, in particular to the technical field of degradation of alkanes below C8.
Background
The petrochemical industry is a pillar industry in China, and plays a significant role in national economy development. The atmospheric pollutants discharged by the petrochemical industry mainly comprise SO 2 NOx, volatile Organic Compounds (VOCs), and the like. The VOCs are various in variety, strong in toxicity and large in harm, are important precursors for generating ozone and secondary organic particles, are also the main culprit for causing regional environmental problems such as photochemical smog and haze, seriously threaten human health and ecological safety, and become important prevention and control objects in petrochemical industry.
2-Methylpentane (2-Methylpentane), also known as isohexane, is widely used as a typical volatile organic compound in petrochemical industry such as petroleum refining, chemical raw materials, synthetic rubber and the like, and has high toxicity and irritation. The human body can feel burning, cough, wheezing, laryngitis, shortness of breath, headache, nausea and vomiting after contacting, and the human body health is endangered, and the environment such as the atmosphere, the soil and the like is endangered to a certain extent.
2-methylpentane can be biodegradable in the environment. Biological purification is the conversion of VOCs to CO by microbial metabolism 2 、H 2 The O process has the advantages of low cost, high removal efficiency, no secondary pollution and the like, and has become one of research hotspots in the technical field of air pollution control. However, a highly efficient degradation strain capable of using 2-methylpentane and the like as a carbon source and an energy source has not been found yet.
Disclosure of Invention
In order to solve the technical problems, the invention provides mycobacterium JY-1. The mycobacterium JY-1 provided by the invention can take 2-methylpentane and analogues thereof as carbon sources and energy sources to realize the efficient degradation of the 2-methylpentane, and has important significance for engineering application of purifying the 2-methylpentane by a biological method.
The specific technical scheme of the invention is as follows:
in a first aspect, the present invention provides a Mycobacterium species which can use 2-methylpentane as a carbon and energy source, said Mycobacterium species being designated JY-1. The Mycobacterium JY-1 was preserved in China center for type culture collection (CCTCC No) at 2022, 2 and 25 days: m2022152, microorganism Classification designated as MycobacteriumMycobacteriumsp.。
The mycobacterium is separated from activated sludge of a sewage treatment plant in Zhejiang, is aerobic gram-positive bacteria, and has small spots, yellow color, opacity, full shape, smoothness and wetness, easy picking up and growth of lawn along a streak. After 16S rDNA sequencing, it was judged to be Mycobacterium and designated asMycobacteriumsp. JY-1。
The mycobacterium JY-1 can grow and reproduce by taking 2-methylpentane as a carbon source and an energy source, can degrade the 2-methylpentane efficiently, and can adapt to a wider temperature and pH range. In addition, the mycobacterium JY-1 can also take 3-methylpentane and n-hexane as carbon sources and energy sources for growth and reproduction, and can efficiently degrade the 3-methylpentane and the n-hexane.
In a second aspect, the invention provides the use of said Mycobacterium JY-1 in degrading organic contaminants.
Preferably, the organic contaminant is at least one selected from the group consisting of 2-methylpentane, 3-methylpentane, and n-hexane.
In a third aspect, the present invention provides a method of degrading an organic contaminant.
A method of degrading organic contaminants comprising the steps of:
a. inoculating the mycobacterium to a solid slant culture medium, and culturing at 25-30 ℃ for 48-72 h to obtain slant thalli;
b. inoculating the slant bacterial cells into an inorganic salt culture medium, and culturing at 25-30 ℃ for 24-36 h to obtain OD 600 Bacterial liquid of 0.1-0.2;
c. mixing the bacterial liquid with the organic pollutants to prepare a mixed liquid;
d. and (3) degrading the mixed solution at 20-40 ℃.
Preferably, the pH of the inorganic salt medium is 4.0 to 9.0.
Preferably, the pH of the inorganic salt medium is 6.0 to 7.0.
As a preferable mode of the above technical scheme, the temperature of the degradation reaction is 25-30 ℃.
When the initial pH is 6.0-7.0 and the temperature is 25-30 ℃, the Mycobacterium JY-1 has a faster propagation speed, and can realize the rapid degradation of 2-methylpentane.
Preferably, the organic contaminant in the mixed solution is at least one selected from 2-methylpentane, 3-methylpentane and n-hexane.
As a preferable mode of the technical scheme, the concentration of the organic pollutants is 30-300 mg/L.
As a preferable mode of the above technical scheme, the mixed solution contains 2-methylpentane with the concentration of 27-135 mg/L, 3-methylpentane with the concentration of 54mg/L and/or n-hexane with the concentration of 53 mg/L.
As a preferable mode of the above technical scheme, the mixed solution contains 2-methylpentane with the concentration of 54-135 mg/L.
When the content of 2-methylpentane is 54-135 mg/L, the mycobacterium JY-1 can grow and reproduce rapidly in a short time, so that the efficient degradation of 2-methylpentane is realized.
As a preferable mode of the above technical scheme, the solid slant culture medium comprises the following components in parts by mass: 0.50-1.00 g/L yeast powder, 0.50-1.00 g/L, mgSO soluble starch 4 0.50-1.00 g/L, 0.50-1.00 g/L tryptone, 0.50-1.00 g/L glucose, 0.30-0.60 g/L, K sodium pyruvate 2 HPO 4 0.45-0.90 g/L, 18-20 g/L agar, and deionized water as solvent.
As a preferable aspect of the above technical scheme, the inorganic salt medium comprises the following components in parts by mass: na (Na) 2 HPO 4 4.0～5.0 g/L、KH 2 PO 4 0.8～1.2 g/L，(NH 4 ) 2 SO 4 2.3～2.8 g/L，MgSO 4 0.18～0.23 g/L、CaCl 2 0.022 to 0.24/g/L, 0.8 to 1.2/mL/L of microelement mother liquor and deionized water as solvent.
As a preferable aspect of the above technical scheme, the inorganic salt medium comprises the following components in parts by mass: na (Na) 2 HPO 4 4.0～5.0 g/L、KH 2 PO 4 0.8～1.2 g/L，(NH 4 ) 2 SO 4 2.3～2.8 g/L，MgSO 4 0.18～0.23 g/L、CaCl 2 0.022 to 0.24/g/L, 0.8 to 1.2/mL/L of microelement mother liquor and deionized water as solvent.
Compared with the prior art, the invention has the following beneficial effects:
1) The mycobacterium JY-1 of the invention can take 2-methylpentane, 3-methylpentane and n-hexane as carbon sources and energy sources to realize the high-efficiency degradation of 2-methylpentane and 3-methylpentane; 48 h, the degradation rate of the catalyst to 2-methylpentane can reach more than 99%, the degradation rate of the catalyst to 3-methylpentane can reach more than 80%, and the degradation rate of the catalyst to n-hexane can reach more than 60%;
2) The Mycobacterium JY-1 of the invention can adapt to a wide temperature and pH range, can grow and reproduce at 20-40 ℃ and pH 4.0-9.0, and can degrade 2-methylpentane to a certain extent.
Drawings
FIG. 1 is a phylogenetic tree of Mycobacterium JY-1;
FIG. 2 is a transmission electron micrograph of Mycobacterium JY-1;
FIG. 3 is a graph showing the degradation of 2-methylpentane by bacterial growth of Mycobacterium JY-1;
FIG. 4 is a bar graph showing the effect of culture solutions of different pH values on degradation (panel A), growth (panel B) and mineralization (panel C) of 2-methylpentane of Mycobacterium JY-1;
FIG. 5 is a bar graph showing the effect of different temperatures on degradation (panel A), growth (panel B) and mineralization (panel C) of 2-methylpentane of Mycobacterium JY-1;
FIG. 6 is a graph showing degradation of Mycobacterium JY-1 against various initial concentrations of 2-methylpentane;
FIG. 7 shows the growth curves of Mycobacterium JY-1 at different initial 2-methylpentane concentrations.
Detailed Description
The invention is further described below with reference to examples.
The ingredients of each medium used in the following examples were as follows:
inorganic salt culture medium: na (Na) 2 HPO 4 ·12H 2 O 4.5 g/L、KH 2 PO 4 1.0 g/L、(NH 4 ) 2 SO 4 2.5 g/L、MgSO 4 ·7H 2 O0.2 g/L, anhydrous CaCl 2 0.023 g/L, microelement mother liquor 1 mL/L, pH7.0, and deionized water as solvent; the trace element mother liquor comprises the following components: feSO 4 ·7H 2 O 1.0 g/L、CuSO 4 ·5H 2 O 0.02 g/L、H 3 BO 3 0.014 g/L、MnSO 4 ·4H 2 O 0.10 g/L、ZnSO 4 ·7H 2 O 0.10 g/L、Na 2 MoO 4 ·2H 2 O 0.02 g/L、CoCl 2 ·6H 2 O0.02 g/L, wherein the solvent is deionized water;
R 2 a solid slant culture medium: yeast powder 0.50 g/L, soluble starch 0.50 g/L, mgSO 4 7H2O 0.50 g/L, tryptone 0.50 g/L, glucose 0.50 g/L, sodium pyruvate 0.30g/L, K HPO 4 ·3H 2 O0.45 g/L, agar 18 g/L, deionized water as solvent and natural pH.
Example 1: isolation, purification and identification of Mycobacterium JY-1
(1) Separation and purification of Mycobacterium JY-1
The method comprises the steps of collecting activated sludge of a sewage treatment plant in Zhejiang on site, mixing the lower sludge after standing with an inorganic salt culture medium according to a ratio of 1:2 (v/v), inoculating 3L mixture into a 5L sludge acclimation tank, using 2-methylpentane as a substrate as a carbon source and energy source, performing acclimation culture at room temperature, taking 5 mL sludge from the acclimation tank after approximately 20 days, adding the 5 mL sludge into a shake flask containing 50 mL inorganic salt culture medium, and finding that the acclimated sludge can stably degrade 27 mg/L2-methylpentane every day in the shake flask, wherein the degradation rate can reach 80-90% (shake flask experimental conditions: 30 ℃ and 160 r/min), thus obtaining acclimated samples.
1, 2 mL of sludge samples in the shake flask are respectively transferred into other shake flasks for further testing performance, the transferred sludge is found to be capable of being degraded into 27 mg/L2-methylpentane stably every day in the shake flask, the sludge samples are continuously transferred and enriched for 6 generations (1 mL is transferred each time), and then the sludge after the 6 generations is enriched is processed according to 10 percent -1 ～10 -6 Performing plate coating multiple times, selecting single colony, taking 2-methylpentane as a substrate, performing degradation activity measurement, and separating and purifying to obtain a strain JY-1 with 2-methylpentane degradation activity.
(2) Identification of Mycobacterium JY-1
Bacterial colony of strain JY-1 is small dot-shaped, yellow, opaque, full in shape, smooth and moist, easy to pick up and fungus coating grows along a streak.
The physiological and biochemical characteristics of the strain JY-1 are as follows: aerobic, gram staining was positive.
The strain JY-1 was subjected to 16S rDNA sequencing, which was performed by the division of biological engineering (Shanghai). 16S rDNA of strain JY-1 (SEQ ID NO. 1) was as follows (Genbank accession OM 721770):
CATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAAGGCCCTTCGGGGTACTCGAGTGGCGAACGGGTGAGTAACACGTGGGTGATCTGCCCTGCACTTTGGGATAAGCCTGGGAAACTGGGTCTAATACCGAATATGATCATGGCCTGCATGGGTTGTGGTGGAAAGCTTTTGCGGTGTGGGATGGGCCCGCGGCCTATCAGCTTGTTGGTGGGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGTGACCGGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGTAGGGACGAAGCGCAAGTGACGGTACCTATAGAAGAAGGACCGGCCAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTAGGTGGTTTGTCGCGTTGTTCGTGAAAACTCACAGCTCAACTGTGGGCGTGCGGGCGATACGGGCAGACTTGAGTACTGCAGGGGAGACTGGAATTCCTGGTGTAGCGGTGGAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGGTCTCTGGGCAGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGTACTAGGTGTGGGTTTCCTTCCTTGGGATCCGTGCCGTAGCTAACGCATTAAGTACCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGAAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTGGATTAATTCGATGCAACGCGAAGAACCTTACCTGGGTTTGACATGCACAGGACGCCGGCAGAGATGTCGGTTCCCTTGTGGCCTGTGTGCAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCTCATGTTGCCAGCACGTTATGGTGGGGACTCGTGAGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGCCCCTTATGTCCAGGGCTTCACACATGCTACAATGGCCGGTACAAAGGGCTGCGATGCCGTGAGGTGGAGCGAATCCTTTCAAAGCCGGTCTCAGTTCGGATCGGGGTCTGCAACTCGACCCCGTGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCAACGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCATGAAAGTCGGTAACACCCGAAGCCGGTGGCCTAACCCCT
the strain JY-1 is identified as Mycobacterium by uploading the 16S rDNA sequence of the strain JY-1 to the Ezbiocloud website, comparing the strain with a standard strain on the website, constructing a bacterial developmental evolutionary tree by adopting a Neighbor-Joing method by using MEGA-X software, evaluating the bacterial developmental evolutionary tree by adopting a Bootstrap method (repeated 1000 times), and determining that the strain JY-1 is Mycobacterium, named MycobacteriumMycobacteriumsp.) JY-1 deposited in China center for type culture Collection with the date of deposition of 2022, 2 and 25 days with the deposit numberCctccc No: m2022152, address: chinese, university of Wuhan, post code 430072.
Example 2: expanded culture of Mycobacterium JY-1
After the Mycobacterium JY-1 is subjected to expansion culture, bacterial liquid containing the Mycobacterium JY-1 is obtained, and the specific process is as follows:
1) Slant culture: inoculating Mycobacterium JY-1 to R 2 Culturing 72 h by using a solid slant culture medium at 30 ℃ to obtain slant bacterial cells;
2) And (3) performing expansion culture: inoculating the slant thallus obtained in step 1) into inorganic salt culture medium at 30deg.C, and culturing at 24 h to obtain OD 600 Bacterial liquid=0.1.
Example 3: performance of Mycobacterium JY-1 to degrade 2-methylpentane
The bacterial liquid obtained in example 2 was inoculated into a fresh 50 mL inorganic salt medium (ph=7) containing 54 mg/L2-methylpentane to give an initial cell concentration of OD 600 Calculated as 0.02. 2 replicates and a blank without strain were designed. Culturing in a shaker at 30deg.C and rotation speed of 160 r/min, sampling every 3-6 hr to determine degradation rate of 2-methylpentane, extracting a part of bacterial liquid with 5 mL syringe, and determining OD value of bacterial cells, and the result is shown in figure 3.
As can be seen from fig. 3: as the time was prolonged, the cell concentration was gradually increased, and the cell concentration of 30 h was cultured to the maximum (about OD 600 =0.15), the degradation rate of 2-methylpentane reaches more than 80%. The mycobacterium JY-1 can utilize 2-methylpentane as a carbon source and an energy source for growth and reproduction, and has the capability of stably and efficiently degrading the 2-methylpentane.
Example 4: effect of initial pH on degradation of 2-methylpentane by Mycobacterium JY-1
With 1 mol/L NaOH aqueous solution or 1 mol/L H 2 SO 4 Adjusting the inorganic salt culture medium to different pH values (4.0, 5.0, 6.0, 7.0, 8.0 and 9.0) by using the aqueous solution; the bacterial liquid obtained in example 2 was inoculated into a fresh inorganic salt medium containing 54 mg/L2-methylpentane and adjusted to a pH of 50: 50 mL, and the initial bacterial cell concentration was set at OD 600 Calculated as 0.02. Design 2Parallel and a blank without strain. Shaking culture of the sample in a shaking table at 30deg.C and 160 r/min for 48 h, sampling, and measuring degradation rate and OD of 2-methylpentane in the reaction solution 600 Value and CO 2 Values, results are shown in FIG. 4.
As can be seen from fig. 4: in the pH=4.0-9.0 range, the mycobacterium JY-1 can grow and reproduce by taking 2-methylpentane as a carbon source and an energy source, and degrade the 2-methylpentane to a certain extent; in the pH=6.0-7.0 range, the Mycobacterium JY-1 has higher degradation rate (more than 99%) to 2-methylpentane. Simultaneous growth of Mycobacterium JY-1 and CO produced 2 The values also showed a trend consistent with the degradation rate of 2-methylpentane.
Example 5: effect of temperature on Mycobacterium JY-1 degradation of 2-methylpentane
The bacterial liquid obtained in example 2 was inoculated into a fresh 50 mL inorganic salt medium (ph=7) containing 54 mg/L2-methylpentane to give an initial cell concentration of OD 600 Calculated as 0.02. The samples were cultured by shaking at constant temperature (shaking rotation speed of 160 r/min) in shaking table at 20deg.C, 25deg.C, 30deg.C, 35deg.C and 40deg.C. At each temperature 2 replicates and a blank without strain were designed. After 20 h culture, sampling is carried out, and the degradation rate and OD of 2-methylpentane in the reaction solution are measured 600 Value and CO 2 Values, results are shown in FIG. 5.
As can be seen from fig. 5: in the temperature range of 20-40 ℃, the mycobacterium JY-1 can grow and reproduce by taking 2-methylpentane as a carbon source and an energy source, and degrade the 2-methylpentane to a certain extent; the Mycobacterium JY-1 has higher degradation rate (more than 99 percent) to 2-methylpentane in the temperature range of 25-30 ℃, and simultaneously the growth amount of the Mycobacterium JY-1 and the generated CO 2 The values also have a tendency to be consistent with the degradation rate of 2-methylpentane.
Example 6: effect of substrate concentration on Mycobacterium JY-1 degradation of 2-methylpentane
Adding different concentrations of substrate 2-methylpentane into fresh inorganic salt culture medium to make initial concentrations of substrate 27, 54, 81, 108, 135mg/L respectively, inoculating bacterial solutions prepared in example 2 respectivelyThe initial cell concentration was set at OD 600 Calculated as 0.02. 2 replicates and a blank without strain were designed. Culturing at 30deg.C in shaking table with rotation speed of 160 r/min, and periodically sampling to determine 2-methylpentane concentration and OD of Mycobacterium JY-1 600 The results are shown in FIGS. 6 and 7, respectively.
As can be seen from fig. 6 and 7: when the concentration of 2-methylpentane is 27-135 mg/L, the Mycobacterium JY-1 can be rapidly propagated in 24 h, and can degrade 2-methylpentane almost completely after 40 h; as the concentration of 2-methylpentane increases, the complete degradation time also increases to some extent.
Example 7: degradation capability of Mycobacterium JY-1 on different carbon source substrates
The bacterial solutions prepared in example 2 were inoculated with a substrate other than 2-methylpentane (substrate concentrations shown in Table 1) in a fresh inorganic salt medium to give an initial bacterial cell concentration of OD 600 Calculated as 0.02. Culturing at 30deg.C and rotation speed of 160 r/min in shaking table under constant temperature shaking, culturing for 48 h, and detecting OD 600 And degradation rate, the results are shown in Table 1. Mycobacterium JY-1 was found to degrade 3-methylpentane and n-hexane to varying degrees.
TABLE 1 degradation capability of Mycobacterium JY-1 on different carbon source substrates
The raw materials and equipment used in the invention are common raw materials and equipment in the field unless specified otherwise; the methods used in the present invention are conventional in the art unless otherwise specified.
The foregoing description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and any simple modification, variation and equivalent transformation of the above embodiment according to the technical substance of the present invention still fall within the scope of the technical solution of the present invention.
Sequence listing
<110> Zhejiang university of industry
<120> Mycobacterium JY-1 and application thereof in degrading organic pollutants
<141> 2022-04-07
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ggataagcct gggaaactgg gtctaatacc gaatatgatc atggcctgca tgggttgtgg 180
tggaaagctt ttgcggtgtg ggatgggccc gcggcctatc agcttgttgg tggggtaatg 240
gcctaccaag gcgacgacgg gtagccggcc tgagagggtg accggccaca ctgggactga 300
gatacggccc agactcctac gggaggcagc agtggggaat attgcacaat gggcgcaagc 360
ctgatgcagc gacgccgcgt gagggatgac ggccttcggg ttgtaaacct ctttcagtag 420
ggacgaagcg caagtgacgg tacctataga agaaggaccg gccaactacg tgccagcagc 480
cgcggtaata cgtagggtcc gagcgttgtc cggaattact gggcgtaaag agctcgtagg 540
tggtttgtcg cgttgttcgt gaaaactcac agctcaactg tgggcgtgcg ggcgatacgg 600
gcagacttga gtactgcagg ggagactgga attcctggtg tagcggtgga atgcgcagat 660
atcaggagga acaccggtgg cgaaggcggg tctctgggca gtaactgacg ctgaggagcg 720
aaagcgtggg gagcgaacag gattagatac cctggtagtc cacgccgtaa acggtgggta 780
ctaggtgtgg gtttccttcc ttgggatccg tgccgtagct aacgcattaa gtaccccgcc 840
tggggagtac ggccgcaagg ctaaaactca aagaaattga cgggggcccg cacaagcggc 900
ggagcatgtg gattaattcg atgcaacgcg aagaacctta cctgggtttg acatgcacag 960
gacgccggca gagatgtcgg ttcccttgtg gcctgtgtgc aggtggtgca tggctgtcgt 1020
cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct tgtctcatgt 1080
tgccagcacg ttatggtggg gactcgtgag agactgccgg ggtcaactcg gaggaaggtg 1140
gggatgacgt caagtcatca tgccccttat gtccagggct tcacacatgc tacaatggcc 1200
ggtacaaagg gctgcgatgc cgtgaggtgg agcgaatcct ttcaaagccg gtctcagttc 1260
ggatcggggt ctgcaactcg accccgtgaa gtcggagtcg ctagtaatcg cagatcagca 1320
acgctgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacgtc atgaaagtcg 1380
gtaacacccg aagccggtgg cctaacccct 1410

Claims (4)

1. The mycobacterium JY-1 is characterized in that the mycobacterium JY-1 has been preserved in China Center for Type Culture Collection (CCTCC) in 2.25.2022 with the preservation number of CCTCC No: m2022152, microorganism Classification designated as MycobacteriumMycobacterium sp.。
2. Use of the mycobacterium JY-1 of claim 1 for degrading organic pollutants; the organic pollutant is at least one selected from 2-methylpentane, 3-methylpentane and n-hexane.
3. A method of degrading organic contaminants comprising the steps of:
a. inoculating the mycobacterium of claim 1 to a solid slant culture medium, and culturing at 25-30 ℃ for 48-72 h to obtain slant bacteria;
b. inoculating the slant bacterial cells into an inorganic salt culture medium, and culturing at 25-30 ℃ for 24-36 h to obtain OD 600 Bacterial liquid of 0.1-0.2;
c. mixing the bacterial liquid with the organic pollutants to prepare a mixed liquid;
d. the mixed solution is subjected to degradation reaction at 20-40 ℃;
the organic pollutant is 2-methylpentane;
the concentration of the organic pollutants is 27-135 mg/L;
the pH value of the inorganic salt culture medium is 4.0-9.0;
the inorganic salt culture medium comprises the following components in parts by mass: na (Na) 2 HPO 4 4.0～5.0 g/L、KH 2 PO 4 0.8～1.2 g/L，(NH 4 ) 2 SO 4 2.3～2.8 g/L，MgSO 4 0.18～0.23 g/L、CaCl 2 0.022 to 0.24/g/L, 0.8 to 1.2/mL/L of microelement mother liquor and deionized water as solvent;
the trace element mother solution comprises the following components: feSO 4 0.8～1.2 g/L、CuSO 4 0.015～0.025 g/L、H 3 BO 3 0.013～0.015 g/L、MnSO 4 0.08～0.13 g/L、ZnSO 4 0.08～0.13 g/L、Na 2 MoO 4 0.015～0.025 g/L、CoCl 2 0.015-0.025 g/L, and deionized water as solvent.
4. A method according to claim 3, characterized in that:
the solid slant culture medium comprises the following components in parts by mass: 0.50-1.00 g/L yeast powder, 0.50-1.00 g/L, mgSO soluble starch 4 0.50-1.00 g/L, 0.50-1.00 g/L tryptone, 0.50-1.00 g/L glucose, 0.30-0.60 g/L, K sodium pyruvate 2 HPO 4 0.45-0.90 g/L, 18-20 g/L of agar and deionized water as solvent.
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