CN114674970A - One-plate multi-information rapid thin-layer chromatography identification and inspection method for ginseng spleen-invigorating pills - Google Patents
One-plate multi-information rapid thin-layer chromatography identification and inspection method for ginseng spleen-invigorating pills Download PDFInfo
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Abstract
The invention discloses a one-plate multi-information rapid thin-layer chromatography identification and inspection method of ginseng spleen-invigorating pills, which relates to the technical field of traditional Chinese medicine identification and comprises the following steps: cutting Ginseng radix-Angelicae-spleen pill, adding methanol, heating under reflux, evaporating to dryness, dissolving the residue in water, passing through macroporous adsorbent resin column, sequentially eluting with water, 20% ethanol and 70% ethanol, collecting 70% ethanol eluate, evaporating to dryness, dissolving the residue in water, extracting with water saturated n-butanol under shaking, washing with ammonia test solution, loading on neutral alumina column, eluting with 40% methanol, and dissolving the residue in methanol to obtain sample; preparation of reference substance, thin layer chromatography. The method has the advantages that the same sample and the same developing agent are adopted, 3 medicinal materials of ginseng, astragalus and spina date seeds are identified on the same thin-layer plate, whether American ginseng is used for replacing or adulterating the ginseng medicinal material or whether jujube kernel or hovenia dulcis thunb is used for replacing the spina date seed is checked, spots are clear, the separation degree is good, and the method is rapid, convenient, efficient, accurate, stable and reliable.
Description
Technical Field
The invention relates to the technical field of thin-layer identification and detection of traditional Chinese medicines, in particular to a one-plate multi-information rapid thin-layer chromatography identification and inspection method of a ginseng spleen-invigorating pill.
Background
The ginseng spleen-invigorating pill has the effects of tonifying qi and nourishing blood, and strengthening spleen and nourishing heart. It is indicated for palpitation, insomnia, amnesia, anorexia, tiredness, sallow complexion due to deficiency of both heart and spleen and insufficiency of qi and blood, and hematochezia, metrorrhagia, leukorrhagia due to spleen failing to control blood.
The existing quality standards of the ginseng spleen-invigorating pills only comprise microscopic identification and thin-layer chromatography identification of an angelica sinensis medicinal material, and no other requirements for detecting components exist. The specific contents are as follows:
[ prescription ] Ginseng radix 80g Atractylodis rhizoma (parched with bran) 160g Poria 160g Glycyrrhrizae radix (baked with honey) 40g radix astragali (baked with honey) 80g radix Angelicae sinensis 160g radix aucklandiae 40g cortex et radix Polygalae (baked with heart-removed Glycyrrhrizae radix) 160g arillus longan 160g semen Ziziphi Spinosae (parched) 80g
[ IDENTIFICATION ] 9g of this product was collected, chopped, added with 40ml of chloroform, refluxed for 0.5 hours under heating, filtered, evaporated to dryness, and the residue was dissolved in 1ml of methanol to give a test solution. Another control medicinal material 0.5g is prepared, and the same method is used to prepare control medicinal solution. According to a thin-layer chromatography test, 5 μ l of each of the two solutions is sucked, and respectively spotted on the same silica gel G thin-layer plate, and n-hexane-ethyl acetate (9: 1) is used as a developing agent, and the solution is developed, taken out, dried and inspected under an ultraviolet lamp (365 nm). In the chromatogram of the test solution, fluorescent spots of the same color appear at the corresponding positions of the chromatogram of the reference solution.
The standard only collects and carries one medicinal material of angelica sinensis, is too simple and is difficult to control the internal quality of the product. In a traditional Chinese medicine compound preparation, particularly a traditional big honeyed pill, because more honey is contained, the extraction of medicinal materials is greatly interfered, the prescription of the compound preparation has more medicinal ingredients, rich chemical components and serious mutual interference, and is limited by the technical difficulty. Thus, the detection period is long, time and labor are wasted, and the detection speed seriously restricts the development speed of modernization of the traditional Chinese medicine. Therefore, the search for a rapid, simple, efficient, stable and low-cost detection method is a bottleneck which must be broken through in the modernization of the quality control of traditional Chinese medicines.
In the formula of the ginseng spleen-invigorating pill, ginseng is the dried root and rhizome of Panax ginseng C.A.Mey. in Araliaceae, is sweet in nature, slightly bitter and slightly warm, and has the effects of invigorating primordial qi, recovering pulse, relieving depletion, invigorating spleen, benefiting lung and the like; the American ginseng is dry root of Panax quinquefolium L. of Araliaceae, is sweet, slightly bitter and cool, and has effects of invigorating qi, nourishing yin, clearing heat and promoting fluid production. The two medicinal materials have different properties and tastes, functional indications and clinical applications, and literature data shows that the chemical substance basis is also obviously different, so that uncertain risks are brought to the effectiveness of the preparation if American ginseng is used as ginseng for feeding. Ginseng and American ginseng are medicinal materials of the same family, and have similar appearance and tissue structure and are easy to be confused. Meanwhile, the ginsenoside Rb is commonly used in the existing preparation standard1Ginsenoside Rg1And ginsenoside Re for quality control, and ginsenoside Re and ginsenoside Rb in radix Panacis Quinquefolii1The content of the ginseng is far higher than that of the ginseng, and the ginseng leftover materials are mixed into the ginseng for batch production, so that the requirement of the limit of the content of the ginseng in the preparation can be easily met. Therefore, there is a possibility of adding ginseng to American ginseng or its leftover. In addition, semen Ziziphi Spinosae (Rhamnaceae plant Ziziphi Spinosae semen Ziziphi Spinosae) in the prescription of Ginseng radix GUIPI pillZiziphus jujuba Mill. var. spinosaDried mature seeds of (Bunge) Hu ex H.F.Chou), higher price, market place for itConfused physical jujube kernel (Rhamnaceae plant Choerospondias yunnanensis)Ziziphus mauritiana lam.Dried mature seed of Hovenia dulcis Thunb) and Hovenia dulcis Thunb (Hovenia dulcis Thunb of Rhamnaceae)Hovenia dulcis Thunnb.Dried mature seeds) is only one fourth of the price of the spina date seeds, and the three have similar appearances, so that most enterprises use the spina date seeds and the hovenia dulcis thunb to replace the spina date seeds for feeding.
In order to effectively control the product quality, ensure the clinical curative effect and achieve the effect of meeting the supervision and rapid inspection, a detection method capable of accurately and rapidly detecting the effective components in the ginseng spleen-invigorating pills needs to be researched and designed.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and aims at the current one variety, namely a traditional identification mode that N samples are prepared, N thin-layer plates are used, and N times of identification are developed. Through the purification treatment of the pretreatment, the problem that the extraction of medicinal materials is greatly interfered due to more honey contained in the traditional big honeyed pills is solved, the problems that the prescription of the compound preparation has more medicinal flavors and similar components are mutually interfered are solved, the possibility that counterfeit medicinal materials of the prescription are adulterated and the production feeding is replaced is simultaneously checked, and the one-plate multi-information rapid thin-layer chromatography identification and inspection method of the ginseng spleen-invigorating pills is provided.
The technical solution of the invention is as follows:
a one-plate multi-information rapid thin-layer chromatography identification and inspection method of a ginseng spleen-invigorating pill is characterized by comprising the following steps:
s1, preparation of a test solution:
collecting Ginseng radix-Angelicae-spleen pill, cutting, adding methanol, heating under reflux, filtering, evaporating filtrate, adding water into residue, heating for dissolving, passing through macroporous adsorbent resin column, sequentially eluting with water, 20% ethanol, and 70% ethanol, collecting 70% ethanol eluate, evaporating, dissolving residue in water, extracting with water saturated n-butanol under shaking, mixing n-butanol solutions, washing with ammonia solution, collecting n-butanol solution, evaporating, dissolving residue with methanol, adding into neutral alumina column, eluting with 40% methanol, collecting eluate, evaporating, dissolving residue with methanol to obtain sample solution;
s2, preparation of control solutions:
collecting semen Ziziphi Spinosae saponin A, semen Ziziphi Spinosae saponin B, and ginsenoside Rg1Ginsenoside Rb1Ginsenoside Re, ginsenoside Rf, and pseudoginsenoside F11And astragaloside IV reference substance, respectively dissolving in methanol to obtain reference substance solution or reference substance mixed solution;
s3, thin layer chromatography:
respectively dripping 5-20 mu L of test solution and 2-5 mu L of each reference solution on the same silica gel G thin-layer plate, developing by using water saturated n-butyl alcohol as a developing agent, taking out, drying in the air, spraying 5% vanillin sulfuric acid test solution, heating until the spots are clearly developed, and inspecting in the sunlight;
s4, judging the result:
in the chromatogram of the test sample, the extract is mixed with spina date seed saponin A, spina date seed saponin B and ginsenoside Rg1Ginsenoside Rb1Spots with the same color appear on the corresponding positions of the chromatogram of the reference substance of ginsenoside Re, ginsenoside Rf and astragaloside IV; mixing with pseudoginsenoside F11Spots with the same color cannot be displayed on the corresponding positions of the reference substances;
specifically, in the chromatogram of the test sample, pseudoginsenoside F is added11If spots with the same color are displayed on the corresponding positions of the reference substance, the use of American ginseng is indicated; in the chromatogram of the test sample, spots with the same color are not shown at the corresponding positions of the jujuboside A reference substance, which indicates that the jujube kernel or the raisin tree seed is used for feeding.
In one embodiment of the present invention, in step S1, the preparing of the test solution specifically includes:
collecting Ginseng radix-Angelicae-spleen pill 4 pills, cutting, adding methanol 150ml, heating under reflux for 1 hr, filtering, evaporating filtrate, adding water 20ml into residue, heating to dissolve, passing through macroporous adsorbent resin column, sequentially eluting with water 70ml, 20% ethanol 70ml, and 70% ethanol 70ml, collecting 70% ethanol eluate, evaporating, adding water 30ml into residue to dissolve, shaking with water saturated n-butanol for 2 times, each time 30ml, mixing n-butanol solutions, washing with ammonia test solution for 2 times, each time 30ml, collecting n-butanol solution, evaporating, dissolving residue with methanol 2ml, loading on neutral alumina column, eluting with 40% methanol 100ml, collecting eluate, evaporating, dissolving residue with methanol lml to obtain sample solution;
in a specific embodiment of the invention, the inner diameter of the macroporous adsorption resin column is 1.5cm, the column height is 15cm, the inner diameter of the neutral alumina column is 1.5cm, the particle size is 100-200 meshes, and the weight is 8 g.
In one embodiment of the invention, the amount of the ginseng spleen-invigorating pill is 3.8g of crude drug per 1 pill.
In a specific embodiment of the present invention, the macroporous adsorbent resin column is a D101 macroporous adsorbent resin column.
In one embodiment of the present invention, in step S2, the control is added with methanol to make a solution containing 1mg of the control per 1ml of methanol.
In one embodiment of the present invention, in step S2, the reference solution is a single reference solution or a mixed solution of a plurality of references.
In one embodiment of the present invention, in step S3, the silica gel G thin layer plate is a high-efficiency silica gel G plate, and the spots are in a stripe shape.
In a specific embodiment of the present invention, in step S3, 5% vanillin sulfuric acid solution is sprayed, and the heating time is 2-6 min.
The principle of the invention is as follows: the applicant proves that the spina date seeds mainly contain spina date seed saponin A and spina date seed saponin B through reference documents and experiments, and the pseudo physical jujube seeds and the hovenia dulcis thunb both only contain the spina date seed saponin B, namely the spina date seed saponin A is not contained. If spots with the same color are not displayed on the positions corresponding to the jujuboside A reference substances in the chromatogram of the test sample, the use of the semen ziziphi spinosae or the feeding of the semen hoveniae is indicated, so that whether the semen ziziphi spinosae is replaced by the semen ziziphi spinosae and the semen hoveniae can be identified. Meanwhile, the characteristic component of ginseng is ginsenoside Rf, and the characteristic component of American ginsengIs divided into pseudoginsenoside F11So that pseudoginsenoside F will be used11As a reference substance, in the chromatogram of the test sample, pseudoginsenoside F is added11And if spots with the same color are displayed on the corresponding positions of the reference substance, the American ginseng is used for feeding. The invention establishes the thin-layer identification method of the special components through the principle, and simultaneously can achieve the purpose of detecting and supervising whether the wild jujube seeds and the ginseng are used for producing the ginseng spleen-invigorating pills according to the prescription process.
The invention has at least one of the following beneficial effects:
1. the invention adopts a one-plate multi-information thin-layer identification and inspection method to identify 7 information spots of 3 medicinal materials (ginseng, astragalus and spina date seeds) in the ginseng spleen-invigorating pill, and can effectively identify the ginseng, the astragalus and the spina date seeds in the prescription; meanwhile, the method can identify prescription drug flavor and check the adulteration and adulteration of the prescription drug flavor, namely, the method can identify that American ginseng is adulterated and jujube kernel or hovenia dulcis thunb is used for replacing wild jujube seed for feeding, thereby providing an effective method for improving the quality standard, ensuring the clinical curative effect and supervising the daily quality of the ginseng spleen-invigorating pills. The invention inspects various information spots on the same sample solution on the same thin-layer plate under the same developing agent condition and the same color development and inspection condition, and has clear result spots, good separation degree, simple and convenient method, high efficiency, good repeatability and high accuracy.
2. According to the invention, the prescription of the sample has more medicinal ingredients and complex chemical components, the honey content is large, the extraction is greatly interfered, in order to remove the honey and effectively separate the components, firstly, according to the principle that the extracted components are saponins, the polarity is large and the extracted components are easy to dissolve in hot methanol, and therefore, the methanol hot reflux is adopted, and the saponin components in the ginseng, the astragalus and the spina date seeds can be fully extracted; secondly, through D101 macroporous adsorption resin, water is sequentially used for elution, so that honey in the test sample can be effectively removed, 20% ethanol is used for elution, so that flavonoid components in the test sample can be effectively removed, 70% ethanol is used for elution, and saponin components in the test sample can be collected; extracting with water saturated n-butanol, removing other low-polarity components by making use of saponin components easily soluble in n-butanol, and further purifying the sample; finally, a neutral alumina column is used for separating saponin components to achieve the final purification purpose. The whole extraction and separation process is scientific and reasonable, has clear thought, and can effectively purify the saponin components in the test sample.
Drawings
Fig. 1 is a thin layer chromatogram of the ginseng spleen-invigorating pill of example 1, wherein,
no. 1 is ginsenoside Rg1Ginsenoside Rb1Ginsenoside Re mixed reference solution;
no. 2 and No. 3 are samples to be tested, and the manufacturer is company A;
no. 4 and No. 7 are samples, and the manufacturer is company B;
no. 5 is pseudoginsenoside F11A control solution;
no. 6 is ginsenoside Rf reference solution;
no. 8 and No. 9 are samples to be tested, and the manufacturer is company C;
no. 10 is mixed reference solution of jujuboside A and jujuboside B;
no. 11 is astragaloside IV control solution.
Detailed Description
The present invention will be described in further detail with reference to specific examples. It will be understood by those skilled in the art that the following examples are illustrative of the present invention only and should not be taken as limiting the scope of the invention. The examples do not indicate any specific techniques or conditions, and the reagents or apparatuses used are not indicated by manufacturers, and are all conventional products commercially available, according to techniques or conditions described in literature in the art or according to the specifications of products. The solution ratios described in the present invention are volume ratios unless otherwise specified.
Example 1
Drugs and reagents:
astragaloside IV (batch No. 110781-202118, content is 96.8%), pseudoginsenoside F11(batch: 110841-202108), ginsenoside Rg1(batch No. 110703-202034, content 94.0%), ginsenoside Rb1(batch No. 110704-202028, content 93.1%)) Ginsenoside Re (batch number: 110754-: 111719-201806), and jujuboside a (batch number: 110734-201713, the content is 95.4 percent), jujuboside B (batch number: 110814 and 201809 in 94.9%) were purchased from the institute of food and drug testing, China.
Ginseng spleen-invigorating pills: ginseng spleen-invigorating pills produced by companies A, B and C are respectively purchased from manufacturers on the market as test products, wherein the batch numbers of the ginseng spleen-invigorating pills produced by the company A are respectively as follows: 170702, 180302; the batch numbers of the ginseng spleen-invigorating pills produced by the company B are respectively as follows: 20161202, 20170202; the batch numbers of the ginseng spleen-invigorating pills produced by company C are respectively as follows: 17051006, 17013852.
Silica gel G high-efficiency thin layer plate. Other reagents were analytically pure.
Taking 4 pills of ginseng spleen-invigorating pills, cutting into pieces, adding 150ml of methanol, heating and refluxing for 1 hour, filtering, evaporating filtrate to dryness, adding 20ml of water into residue, heating to dissolve, passing through a D101 macroporous adsorption resin column (the inner diameter is 1.5cm, the column height is 15cm), eluting with 70ml of water, 70ml of 20% ethanol and 70% ethanol in sequence, collecting 70% ethanol eluate, evaporating to dryness, adding 30ml of water into residue to dissolve, shaking and extracting with water saturated n-butyl alcohol for 2 times, 30ml each time, combining n-butyl alcohol solutions, washing with ammonia test solution for 2 times, 30ml each time, taking n-butyl alcohol solution, evaporating to dryness, dissolving residue with 2ml of methanol, adding eluent on a neutral alumina column (100-200 meshes, 8g, the inner diameter is 1.5cm), eluting with 100ml of 40% methanol, collecting, evaporating to dryness, adding methanol lml into residue to dissolve, and taking the residue as a sample solution. Preparation of control solutions: collecting semen Ziziphi Spinosae saponin A and semen Ziziphi Spinosae saponin B reference substances, adding methanol to obtain mixed solution containing 1mg of each 1ml, collecting ginsenoside Rg1Ginsenoside Rb1Mixing with ginsenoside Re reference substance, adding methanol to obtain mixed solution containing 1mg of ginsenoside Rf and pseudoginsenoside F per 1ml11And astragaloside IV reference substance, respectively adding methanol to make into solution containing 1mg per 1ml as reference substance solution; sucking 10 μ l of sample solution and 2 μ l of each control solution, respectively dropping on the same high efficiency silica gel G thin layer plate to form strip, developing with water saturated n-butanol as developing agent, taking out,air drying, spraying 5% vanillin sulfuric acid solution, heating until the color of spots is clear, and inspecting in sunlight.
And (4) judging a result: in the chromatogram of the test sample, the extract is mixed with spina date seed saponin A, spina date seed saponin B and ginsenoside Rg1Ginsenoside Rb1Spots with the same color should appear on the corresponding positions of the chromatogram of the reference substance of ginsenoside Re, astragaloside IV and ginsenoside Rf; mixing with pseudoginsenoside F11The control product should not show spots of the same color at the corresponding positions.
From the TLC plate analysis in FIG. 1, no ginsenoside Rf reference substance was detected in the samples No. 2 and No. 3 of company A, and pseudoginsenoside F was detected11And (3) detecting chromatographic spots of the reference substances, namely, detecting chromatographic spots of the spina date seed saponin A when chromatographic spots of the reference substances are not detected, and detecting chromatographic spots of other 6 reference substances, namely, the ginseng spleen-invigorating pills produced by using American ginseng leftover materials to replace ginseng and using jujube kernel or hovenia dulcis thumb to replace wild jujube seed.
No. 4 sample 8 reference substances of company B are detected, namely the ginseng spleen-invigorating pill produced by feeding ginseng mixed with American ginseng leftover; no. 7 specimen without detecting pseudoginsenoside F11Detecting the chromatographic spots of the reference substances and the other 7 reference substances, namely the genuine ginseng spleen-invigorating pill.
No. 8 and No. 9 samples of C company did not detect pseudoginsenoside F11Detecting the chromatographic spots of the reference substances and the rest 7 reference substances to obtain the quality-grade ginseng spleen-invigorating pills.
Therefore, the method can identify 7 information spots in the ginseng spleen-invigorating pill, can effectively identify ginseng, astragalus and spina date seeds in the prescription, and can detect the characteristic component pseudo-ginsenoside F in the American ginseng leftover material11The method can detect the adulteration of the medicinal flavor and the adulteration of the materials fed by the prescription, namely, the adulteration of the ginseng with the American ginseng and the feeding of the spina date seed with the semen ziziphi spinosae or the semen hoveniae can be identified, thereby providing an effective method for improving the quality standard, ensuring the clinical curative effect and supervising the daily quality of the ginseng-spleen tonifying pills.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. A one-plate multi-information rapid thin-layer chromatography identification and inspection method of a ginseng spleen-invigorating pill is characterized by comprising the following steps:
s1, preparation of a test solution:
collecting Ginseng radix-Angelicae-spleen pill, cutting, adding methanol, heating under reflux, filtering, evaporating filtrate, adding water into residue, heating for dissolving, passing through macroporous adsorbent resin column, sequentially eluting with water, 20% ethanol, and 70% ethanol, collecting 70% ethanol eluate, evaporating, dissolving residue in water, extracting with water saturated n-butanol under shaking, mixing n-butanol solutions, washing with ammonia solution, collecting n-butanol solution, evaporating, dissolving residue with methanol, adding into neutral alumina column, eluting with 40% methanol, collecting eluate, evaporating, dissolving residue with methanol to obtain sample solution;
s2, preparation of control solutions:
collecting semen Ziziphi Spinosae saponin A, semen Ziziphi Spinosae saponin B, and ginsenoside Rg1Ginsenoside Rb1Ginsenoside Re, ginsenoside Rf, and pseudoginsenoside F11Adding methanol to dissolve astragaloside IV as reference substance solution or reference substance mixed solution;
s3, thin layer chromatography:
respectively dropping 5-20 μ l of the test solution and 2-5 μ l of each reference solution on the same silica gel G thin layer plate, developing with water saturated n-butanol as developing agent, taking out, air drying, spraying 5% vanillin sulfuric acid solution, heating until the spots are clearly developed, and inspecting in sunlight;
s4, judging the result:
in the chromatogram of the test sample, the extract is mixed with spina date seed saponin A, spina date seed saponin B and ginsenoside Rg1Ginsenoside Rb1Spots with the same color appear on the corresponding positions of the chromatogram of the reference substance of ginsenoside Re, ginsenoside Rf and astragaloside IV; in and withPseudo-ginseng saponin F11The control product should not show spots of the same color at the corresponding positions.
2. The method for identifying and checking ginseng spleen-invigorating pills by one-plate multi-information rapid thin-layer chromatography according to claim 1, wherein in step S1, the preparation of the test solution specifically comprises:
collecting Ginseng radix-Angelicae-spleen pill 4 pills, cutting, adding methanol 150ml, heating under reflux for 1 hr, filtering, evaporating filtrate, adding water 20ml into residue, heating to dissolve, passing through macroporous adsorbent resin column, sequentially eluting with water 70ml, 20% ethanol 70ml, and 70% ethanol 70ml, collecting 70% ethanol eluate, evaporating, adding water 30ml into residue to dissolve, shaking with water saturated n-butanol for 2 times, each time 30ml, mixing n-butanol solutions, washing with ammonia test solution for 2 times, each time 30ml, collecting n-butanol solution, evaporating to dry, dissolving residue with methanol 2ml, loading on neutral alumina column, eluting with 40% methanol 100ml, collecting eluate, evaporating to dry, and dissolving residue with methanol lml to obtain test solution.
3. The method for one-plate multi-information rapid thin-layer chromatography identification and inspection of the ginseng spleen-invigorating pills according to claim 2, wherein the inner diameter of a macroporous adsorption resin column is 1.5cm, the column height is 15cm, the inner diameter of a neutral alumina column is 1.5cm, the particle size is 100-200 meshes, and the weight is 8 g.
4. The one-plate multi-information rapid thin-layer chromatography identification and inspection method of the ginseng spleen-invigorating pills according to claim 2, wherein each 1 pill of the ginseng spleen-invigorating pills is equivalent to 3.8g of crude drug.
5. The one-plate multi-information rapid thin-layer chromatography identification and inspection method of the ginseng spleen-invigorating pills according to claim 2, characterized in that a D101 macroporous adsorption resin column is selected as the macroporous adsorption resin column.
6. The method for identification and examination of ginseng spleen-invigorating pill by one-plate multi-information rapid thin-layer chromatography as claimed in claim 1, wherein in step S2, the reference substance is added with methanol to prepare a solution containing 1mg of the reference substance per 1ml of methanol.
7. The identification and examination method of one-plate multi-information rapid thin-layer chromatography of ginseng spleen-invigorating pill according to claim 1, wherein in step S2, the reference solution is a single reference solution or a mixed solution of a plurality of references.
8. The method for single-plate multi-information rapid thin-layer chromatography identification and inspection of ginseng spleen-invigorating pills according to claim 1, wherein in step S3, the silica gel G thin-layer plate is a high-performance silica gel G plate, and the spots are in the shape of stripes.
9. The one-plate multi-information rapid thin-layer chromatography identification and inspection method of the ginseng spleen-invigorating pills according to claim 1, wherein in the step S3, a 5% vanillin sulfuric acid test solution is sprayed, and the heating time is 2-6 min.
10. The method for identification and examination of ginseng spleen-invigorating pill by one-plate multi-information rapid thin-layer chromatography as claimed in claim 1, wherein in step S4, pseudoginsenoside F is added to the sample chromatogram11If spots with the same color are displayed on the corresponding positions of the reference substance, the use of American ginseng is indicated; in the chromatogram of the test sample, spots with the same color are not shown at the corresponding positions of the jujuboside A reference substance, which indicates that the jujube kernel or the raisin tree seed is used for feeding.
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