CN114674892B - Construction of up-conversion material combined signal quenching sensor - Google Patents

Construction of up-conversion material combined signal quenching sensor Download PDF

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CN114674892B
CN114674892B CN202210268552.5A CN202210268552A CN114674892B CN 114674892 B CN114674892 B CN 114674892B CN 202210268552 A CN202210268552 A CN 202210268552A CN 114674892 B CN114674892 B CN 114674892B
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颜梅
苗培
张晶
秦成坤
马廷滨
刘明霞
吕艳锋
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Abstract

The invention discloses a construction method of an up-conversion material combined signal quenching sensor, which firstly synthesizes NaYF which can be excited by near infrared light and has good photoelectrochemical signal 4 :Yb，Er@Bi 2 MoO 6 @Bi, where NaYF 4 Yb, er as one of the up-conversion materials, has excellent light transmittance, low photodamage and high light stability; at the same time at NaYF 4 :Yb，Er@Bi 2 MoO 6 In @ Bi, the plasma Bi is used as a light concentrator and an energy conveyer, so that the absorption section and the interface energy of an up-conversion system can be effectively improved; the sensing system is excited by near infrared light, and the near infrared light has the advantages of low phototoxicity and corrosiveness and minimum photobleaching; further, au@CeO is modified on the secondary antibody 2 As a signal amplification carrier, along with a secondary antibody marker Au@CeO 2 The steric hindrance effect brought by the introduction of (2) can effectively prevent electron transfer, reduce photocurrent, and simultaneously Au@CeO 2 The catalyst can also be used as a simulated peroxidase to effectively catalyze 4-chloro-1-naphthol to generate corresponding sediment, further reduce photocurrent and improve the sensitivity of the sensor.

Description

Construction of up-conversion material combined signal quenching sensor
Technical Field
The invention relates to the field of quantitative detection of prostate specific antigen, in particular to construction of an up-conversion material combined signal quenching sensing device.
Background
At present, the probability of cancer is more and more, but the existing modes of radiotherapy, chemotherapy and the like can generate serious injury to normal cells and irreversible injury to human bodies when treating the cancer. Therefore, the early detection and treatment of the cancer have very important significance for diagnosing the malignant tumor, and the establishment of a novel method for detecting the malignant tumor biomarker with simplicity, rapidness, sensitivity and good selectivity has very important value for early detection and treatment effect evaluation of the malignant tumor.
The up-conversion nano particles have the characteristics of low photodamage, stable chemical components, large stokes shift resistance and the like, and the characteristics make the up-conversion nano particles become perfect candidates for special applications such as medical imaging, biosensing, photo-activated treatment and the like. Photoelectrochemical (PEC) biosensors based on upconverting nanoparticles use infrared light sources instead of ultraviolet and visible light sources to indirectly excite photosensitive materials, and maintain a strong penetration depth and near zero photobleaching, with cost effectiveness, rapid response, and high sensitivity to detection of cancer biomarkers.
Disclosure of Invention
The invention aims to construct a photoelectrochemical sensor for prostate specific antigen detection by using an up-conversion material in combination with a signal quenching sensor device.
In order to solve the technical problems, the invention is realized by the following measures: the construction of the up-conversion material combined signal quenching sensing device is characterized by comprising the following steps:
(1) Synthesis of NaYF 4 Yb, er: trisodium citrate 1.176, g was dissolved in 5, mL water, then 1 mmol of Ln (NO) was added with stirring 3 ) 3 （74.4% Y 3+ ，25% Yb 3+ ，0.1% Er 3+ ) And 3 g NaNO 3 Stirring for 20 min; thereafter, naF (0.252 g NaF in 5 mL water) was added and stirred well; the above solution was transferred to a 20 mL polytetrafluoroethylene-lined autoclave and heated at 180 ℃ for 12 h; the product was then separated by centrifugation and further washed with water and ethanol; finally, it was dried in a vacuum oven at 60 ℃ overnight.
(2) Synthesis of NaYF 4 :Yb，Er@Bi 2 MoO 6 @ Bi: 5.0 mmol Bi (NO) 3 ) 3 ·5H 2 Transferring O and 0.1 g step (1) and the synthesized product into 10 mL glycol, and then ultrasonically stirring for 20 min to form a uniform solution; subsequently, the mixture containing 2.5 mmol Na was stirred magnetically 2 MoO 4 2H 2 Slowly dripping the 10 mL glycol solution of O into the solution and stirring for 30 min; then, atSlowly dripping 40 mL absolute ethyl alcohol into the solution under vigorous stirring, and continuously stirring for 60 min; the resulting mixture was transferred to an autoclave of 100 mL, held at 160 ℃ at 12 h; subsequently, when the autoclave was cooled to room temperature, the product was washed 4 times with ethanol and deionized water, and then dried under vacuum at 80 ℃ for 10 h.
(3) Construction of ITO/NaYF 4 :Yb，Er@Bi 2 MoO 6 @ Bi electrode: the conductive glass is indium tin oxide glass (ITO), the conductive glass is cut into 4.0x0.5 cm strips, sequentially washed by acetone solution, secondary distilled water and absolute ethyl alcohol for 5 min in an ultrasonic manner, and then dried under nitrogen for standby; synthesizing NaYF with concentration of 2.0 mg/mL in the step (2) 4 :Yb，Er@Bi 2 MoO 6 Dripping Bi on ITO glass, drying at 60deg.C to obtain ITO/NaYF 4 :Yb，Er@Bi 2 MoO 6 A @ Bi electrode.
(4) Synthesis of Au@CeO 2 : na of 0.0134 g 3 PO 4 Dissolving in 35 mL ultrapure water and stirring for 10 min, stirring to give Ce (NO) with concentration of 5 mL at 0.2 mol/L 3 ) 3 ·6H 2 O was added dropwise to the above solution, and the mixed solution was transferred to an autoclave and reacted at 200 ℃ for 20 h; after the reaction was completed, a precipitate was obtained by centrifugal separation, and washed with ultrapure water and absolute ethanol several times; subsequently, the sample was transferred to a muffle furnace at a heating rate of 5 ℃ per min and calcined at 600 ℃ for 6 h; will calcine CeO 2 Dissolved in 8 mL of 1% bovine serum albumin aqueous solution and stirred at room temperature for 4 h; collecting the bovine serum albumin coated CeO again by centrifugation 2 It was suspended in 5 mL of Au NPs solution and stirred 12 h to fully absorb Au NPs; the product was then centrifuged at 10000 rpm for 10 min and washed several times with ultra pure water to remove residues; finally, the precipitate is dried at 60 ℃ for 12 h to obtain Au@CeO 2 。
(5) Synthesis of Ab2-Au@CeO 2 : adding Ab2, which is a secondary antibody with the concentration of 1 mL of 10 mug/mL, into the synthesized product of the step (4), incubating for 2 h at 4 ℃, and washing 3 times with phosphate buffer with the pH of 7.4 to remove the Ab2 without complexing, thus obtaining Ab2-Au@CeO 2 。
(6) Construction of a Photosensor (PEC): rinsing ITO/NaYF with ultra pure water 4 :Yb，Er@Bi 2 MoO 6 At Bi electrode, 6. Mu.L of primary antibody Ab1 at a concentration of 10. Mu.g/mL was then incubated at 4℃for 16 h, and thoroughly rinsed 3 times with phosphate buffer at pH 7.4; continuously dripping 20 mu L of 3% bovine serum albumin to block the non-specific binding site, thoroughly flushing 3 times by using phosphate buffer solution with the pH of 7.4, dripping 20 mu L of prostate antigens with different concentrations onto the surface of an electrode, incubating for 30 min at room temperature, and washing 3 times by using the phosphate buffer solution with the pH of 7.4; continuously dripping 20 mu L of Ab2-Au@CeO synthesized in the step (5) 2 Incubating the electrode at room temperature for 4H and incubating the modified electrode at room temperature in the presence of 1 mM H 2 O 2 Is incubated for 20 min with 10 mM 4-chloro-1-naphthol solution.
(7) Electrochemical detection by a photoelectric sensor: the modified electrode treated in the step (6) is used as a working electrode, a counter electrode is a platinum wire electrode, a reference electrode is an Ag/AgCl electrode, the bias value is 0V, an infrared laser (2.0W, 980 nm) is used as a light source for stimulation, an electrolytic cell is a phosphate buffer system (1 mol/L ascorbic acid) with pH of 7.4, and a current I-T curve is measured to detect photoelectric performance.
The invention has the beneficial effects that:
(1) The invention has low cost, simple experimental operation and easy control of reaction conditions.
（2）NaYF 4 Yb, er as one of the up-conversion materials, has excellent light transmittance, low photodamage and high light stability; at the same time NaYF 4 Yb, er converts long wave incident light into short wave incident light with higher energy through an anti-Stokes process, namely, the long wave incident light is used for converting near infrared light into visible light and ultraviolet light.
(3) In NaYF 4 :Yb，Er@Bi 2 MoO 6 In @ Bi, the plasma Bi is used as a light concentrator and an energy conveyer, so that the absorption cross section and the interface energy of the up-conversion system can be effectively improved.
(4) The sensor system is excited by near infrared light, and the near infrared light has the advantages of low phototoxicity and corrosiveness and minimum photobleaching.
（5）Au@CeO 2 The photoelectric layer can compete with the photoelectric layer to capture photon energy and electron donor, quench photocurrent signals, and catalyze the surface of the photoelectric layer to form simulated enzyme catalytic precipitation like peroxidase, so that the detection sensitivity is further improved; in addition, au@CeO 2 The steric hindrance effect of (c) further reduces the output of the photocurrent signal.
Detailed Description
For further understanding of the present invention, embodiments are provided by combining the technical solutions of the present invention with examples:
(1) Synthesis of NaYF 4 Yb, er: trisodium citrate 1.176, g was dissolved in 5, mL water, then 1 mmol of Ln (NO) was added with stirring 3 ) 3 （74.4% Y 3+ ，25% Yb 3+ ，0.1% Er 3+ ) And 3 g NaNO 3 Stirring for 20 min; thereafter, a sodium fluoride solution (0.252 g NaF in 5 mL water) was added and stirred well; the above solution was transferred to a 20 mL polytetrafluoroethylene-lined autoclave and heated at 180 ℃ for 12 h; the product was then separated by centrifugation and further washed with water and ethanol; finally, it was dried in a vacuum oven at 60 ℃ overnight.
(2) Synthesis of NaYF 4 :Yb，Er@Bi 2 MoO 6 @ Bi: 5.0 mmol Bi (NO) 3 ) 3 ·5H 2 Transferring O and 0.1 g step (1) and the synthesized product into 10 mL glycol, and then ultrasonically stirring for 20 min to form a uniform solution; subsequently, the mixture containing 2.5 mmol Na was stirred magnetically 2 MoO 4 2H 2 Slowly dripping the 10 mL glycol solution of O into the solution and stirring for 30 min; then, slowly dripping 40 mL absolute ethyl alcohol into the solution under vigorous stirring, and continuously stirring for 60 min; the resulting mixture was transferred to an autoclave of 100 mL, held at 160 ℃ at 12 h; subsequently, when the autoclave was cooled to room temperature, the product was washed 4 times with ethanol and deionized water, and then dried under vacuum at 80 ℃ for 10 h.
(3) Construction of ITO/NaYF 4 :Yb，Er@Bi 2 MoO 6 @ Bi electrode: the conductive glass is indium tin oxide glass (ITO) and is to be conductiveCutting glass into strips of 4.0X10.5. 0.5 cm, sequentially ultrasonic cleaning with acetone solution, redistilled water and absolute ethyl alcohol for 5 min, and drying under nitrogen for use; synthesizing NaYF with concentration of 2.0 mg/mL in the step (2) 4 :Yb，Er@Bi 2 MoO 6 Dripping Bi on ITO glass, drying at 60deg.C to obtain ITO/NaYF 4 :Yb，Er@Bi 2 MoO 6 A @ Bi electrode.
(4) Synthesis of Au@CeO 2 : na of 0.0134 g 3 PO 4 Dissolving in 35 mL ultrapure water and stirring for 10 min, stirring to give Ce (NO) with concentration of 5 mL at 0.2 mol/L 3 ) 3 ·6H 2 O was added dropwise to the above solution, and the mixed solution was transferred to an autoclave and reacted at 200 ℃ for 20 h; after the reaction was completed, a precipitate was obtained by centrifugal separation, and washed with ultrapure water and absolute ethanol several times; subsequently, the sample was transferred to a muffle furnace at a heating rate of 5 ℃ per min and calcined at 600 ℃ for 6 h; will calcine CeO 2 Dissolved in 8 mL of 1% bovine serum albumin aqueous solution and stirred at room temperature for 4 h; collecting the bovine serum albumin coated CeO again by centrifugation 2 It was suspended in 5 mL of Au NPs solution and stirred 12 h to fully absorb Au NPs; the product was then centrifuged at 10000 rpm for 10 min and washed several times with ultra pure water to remove residues; finally, the precipitate is dried at 60 ℃ for 12 h to obtain Au@CeO 2 。
(5) Synthesis of Ab2-Au@CeO 2 : adding Ab2, which is a secondary antibody with the concentration of 1 mL of 10 mug/mL, into the synthesized product of the step (4), incubating for 2 h at 4 ℃, and washing 3 times with phosphate buffer with the pH of 7.4 to remove the Ab2 without complexing, thus obtaining Ab2-Au@CeO 2 。
(6) Construction of a Photosensor (PEC): rinsing ITO/NaYF with ultra pure water 4 :Yb，Er@Bi 2 MoO 6 At Bi electrode, 6. Mu.L of primary antibody Ab1 at a concentration of 10. Mu.g/mL was then incubated at 4℃for 16 h, and thoroughly rinsed 3 times with phosphate buffer at pH 7.4; continuously dripping 20 mu L of 3% bovine serum albumin to block the non-specific binding site, thoroughly flushing 3 times with phosphate buffer solution with pH of 7.4, dripping 20 mu L of different concentrations of prostate antigen onto the surface of an electrode, and filling a chamberAfter incubation for 30 min at temperature, washing 3 times with phosphate buffer at pH 7.4; continuously dripping 20 mu L of Ab2-Au@CeO synthesized in the step (5) 2 Incubating the electrode at room temperature for 4H and incubating the modified electrode at room temperature in the presence of 1 mM H 2 O 2 Is incubated for 20 min with 10 mM 4-chloro-1-naphthol solution.
(7) Electrochemical detection by a photoelectric sensor: the modified electrode treated in the step (6) is used as a working electrode, a counter electrode is a platinum wire electrode, a reference electrode is an Ag/AgCl electrode, the bias value is 0V, an infrared laser (2.0W, 980 nm) is used as a light source for stimulation, an electrolytic cell is a phosphate buffer system (1 mol/L ascorbic acid) with pH of 7.4, the current I-T curve is measured for detection of photoelectric performance, a linear equation of I= -3.28log (c) -15.04 is obtained, the correlation coefficient is 0.993, the detection limit is 0.07 pg/mL, and the detection of the prostate specific antigen with high sensitivity is realized.

Claims (1)

1. The construction method of the up-conversion material combined signal quenching sensor is characterized by comprising the following steps:
(1) Synthesis of NaYF 4 Yb, er: 1.176 g trisodium citrate was dissolved in 5. 5 mL water, and then 1 mmol of each ion was added with stirring, the contents of which were 74.4% of Y, respectively 3+ 25% Yb 3+ 0.1% Er 3+ Ln (NO) 3 ) 3 And 3 g NaNO 3 Stirring for 20 min to obtain a mixed solution; thereafter, 0.252 g of NaF was dissolved in 5 mL water to obtain a sodium fluoride mixed solution; the two solutions were transferred to a 20 mL polytetrafluoroethylene-lined autoclave and heated at 180 ℃ for 12 h; then separating the product by centrifugation, further washing with water and ethanol; finally, it was dried in a vacuum oven at 60 ℃ overnight;
(2) Synthesis of NaYF 4 :Yb，Er@Bi 2 MoO 6 @ Bi: 5.0 mmol Bi (NO) 3 ) 3 ·5H 2 Transferring O and 0.1 g product synthesized in the step (1) into 10 mL glycol, and then ultrasonically stirring for 20 min to form a uniform solution; subsequently, the mixture containing 2.5 mmol Na was stirred magnetically 2 MoO 4 2H 2 Slowly drop of 10 mL glycol solution of OAdding into the above solution, and stirring for 30 min; then, slowly dripping 40 mL absolute ethyl alcohol into the solution under vigorous stirring, and continuously stirring for 60 min; the resulting mixture was transferred to an autoclave of 100 mL, held at 160 ℃ at 12 h; subsequently, when the autoclave was cooled to room temperature, the product was washed 4 times with ethanol and deionized water, and then dried under vacuum at 80 ℃ for 10 h;
(3) Construction of ITO/NaYF 4 :Yb，Er@Bi 2 MoO 6 @ Bi electrode: the conductive glass is indium tin oxide glass (ITO), the conductive glass is cut into 4.0x0.5 cm strips, sequentially washed by acetone solution, secondary distilled water and absolute ethyl alcohol for 5 min in an ultrasonic manner, and then dried under nitrogen for standby; synthesizing NaYF with concentration of 2.0 mg/mL in the step (2) 4 :Yb，Er@Bi 2 MoO 6 Dripping Bi on ITO glass, drying at 60deg.C to obtain ITO/NaYF 4 :Yb，Er@Bi 2 MoO 6 A @ Bi electrode;
(4) Synthesis of Au@CeO 2 : na of 0.0134 g 3 PO 4 Dissolving in 35 mL ultrapure water and stirring for 10 min, stirring to give Ce (NO) with concentration of 5 mL at 0.2 mol/L 3 ) 3 ·6H 2 O was added dropwise to the above solution, and the mixed solution was transferred to an autoclave and reacted at 200 ℃ for 20 h; after the reaction was completed, a precipitate was obtained by centrifugal separation, and washed with ultrapure water and absolute ethanol several times; subsequently, the sample was transferred to a muffle furnace at a heating rate of 5 ℃/min and calcined at 600 ℃ for 6 h; will calcine CeO 2 Octahedron was dissolved in 8 mL of 1% aqueous bovine serum albumin solution and stirred at room temperature for 4 h; collecting the bovine serum albumin coated CeO again by centrifugation 2 It was suspended in 5 mL of Au NPs solution and stirred 12 h to fully absorb Au NPs; the product was then centrifuged at 10000 rpm for 10 min and washed several times with ultra pure water to remove residues; finally, the precipitate is dried at 60 ℃ for 12 h to obtain Au@CeO 2 ；
(5) Synthesis of Ab2-Au@CeO 2 : adding Ab2 (1 mL) with concentration of 10 μg/mL into the synthesized product of step (4), incubating at 4deg.C for 2 h, washing with phosphate buffer solution with pH of 7.4 for 3 times to remove uncomplexed Ab2, and obtainingTo Ab2-Au@CeO 2 ；
(6) Construction of a Photosensor (PEC): rinsing ITO/NaYF with ultra pure water 4 :Yb，Er@Bi 2 MoO 6 At Bi electrode, 6. Mu.L of primary antibody Ab1 at a concentration of 10. Mu.g/mL was then incubated at 4℃for 16 h, and thoroughly rinsed 3 times with phosphate buffer at pH 7.4; continuously dripping 20 mu L of 3% bovine serum albumin to block the non-specific binding site, thoroughly flushing 3 times by using phosphate buffer solution with the pH of 7.4, dripping 20 mu L of prostate antigens with different concentrations onto the surface of an electrode, incubating for 30 min at room temperature, and washing 3 times by using the phosphate buffer solution with the pH of 7.4; continuously dripping 20 mu L of Ab2-Au@CeO synthesized in the step (5) 2 Incubating the electrode at room temperature for 4H and incubating the modified electrode at room temperature in the presence of 1 mM H 2 O 2 Is incubated for 20 min with 10 mM 4-chloro-1-naphthol solution.
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