CN114672418A - Device suitable for simulating gravity culture cells under microgravity environment - Google Patents

Device suitable for simulating gravity culture cells under microgravity environment Download PDF

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Publication number
CN114672418A
CN114672418A CN202210474817.7A CN202210474817A CN114672418A CN 114672418 A CN114672418 A CN 114672418A CN 202210474817 A CN202210474817 A CN 202210474817A CN 114672418 A CN114672418 A CN 114672418A
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rod
side wall
base
telescopic rod
cell culture
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Inventor
吴琦
李玉婷
张黎
郝艳坤
李爽
王洪伟
郑慧哲
魏韬
李姝�
孙健
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Mudanjiang Medical University
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Mudanjiang Medical University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals

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Abstract

The invention discloses a device suitable for simulating gravity culture cells in a microgravity environment, relates to the field of cell culture, and aims to solve the problems that cell culture in multiple groups of gravity environments cannot be simulated simultaneously under the existing microgravity condition, gas required by cell culture cannot be effectively supplemented, and adjustment of different rotating speeds of cell culture bottles is difficult to realize. The invention drives the turntable to rotate through the motor, and the stepped gear shaft of the rotating mechanism is meshed with the rack on the inner wall of the opening of the turntable. The annular supporting seat is connected with the base through the telescopic adjusting frame of the inverted L-shaped structure, and the adjustment of the horizontal direction and the vertical direction of the stepped gear shaft is realized through adjusting different adjusting rods of the telescopic adjusting frame. Therefore, the racks on the surface of the shaft with different diameters on the stepped gear shaft are in toothed connection with the racks on the inner wall of the turntable, so that the culture dish can rotate at different rotating speeds, and conditions with different gravity can be simulated. The invention is applied to the field of cell culture.

Description

Device suitable for simulating gravity culture cells under microgravity environment
Technical Field
The invention belongs to the field of cell culture, and particularly relates to a device suitable for simulating gravity culture cells in a microgravity environment.
Background
At present, research institutions and scientists in various countries around the world develop various biological tests under microgravity environments. Space cell culture represented by Mesenchymal Stem Cells (MSC), Hematopoietic Stem Cells (HSC), periodontal ligament stem cells (PDLSC), Embryonic Stem Cells (ESC), and the like has become one of the hot spots of current research. Research shows that the MSCs are not only important regulators for maintaining the content of bone cells and myoblasts in vivo, but also have the advantages of immune compatibility, directional migration to damaged sites to participate in tissue repair and the like. The symptoms of muscular system atrophy, osteoporosis and the like of a human body under the microgravity environment are closely related to the biological behavior of the organism regulated by the MSCs.
In order to study the culture of the cells under microgravity, it is necessary to simulate the gravity environment in microgravity environment and perform bidirectional study to better study the mechanism. However, the conventional microgravity cell culture apparatus cannot simultaneously culture a plurality of cells in a simulated ground gravity environment, and cannot simultaneously supply a plurality of culture bottles with a required gas component without stopping the apparatus in the simulated gravity environment.
Disclosure of Invention
The invention aims to solve the problems that cell culture in a multi-group gravity environment cannot be simulated simultaneously under the existing microgravity condition, gas required by the cell culture cannot be effectively supplemented, and adjustment of different rotating speeds of a cell culture bottle is difficult to realize. And provides a device suitable for simulating gravity culture cells in a microgravity environment.
The invention relates to a device for simulating gravity culture of cells in a microgravity environment, which comprises a base, a turntable, a driving mechanism, a plurality of culture dish rotating mechanisms and a plurality of regulators, wherein the turntable is arranged on the base;
the culture dish rotating mechanism consists of a stepped gear shaft, an annular supporting seat, a rolling rod and a culture dish; the diameter of the stepped gear shaft is gradually increased from bottom to top, a rack is arranged on the surface of the stepped gear shaft, and the top end of the stepped gear shaft is connected with the bottom of the U-shaped seat; the inner wall of the annular supporting seat is circumferentially provided with a roller holding groove, and the roller is arranged in the roller holding groove; the U-shaped seat is arranged in the annular supporting seat, and the outer side wall of the U-shaped seat is in rolling connection with the roller; the culture dish is placed in the U-shaped seat;
the driving mechanism consists of a motor, a first transmission gear, a second transmission gear and a rotating shaft which are arranged in the base; the first transmission gear is arranged on a power output shaft of the motor and is in two-tooth connection with the transmission gear arranged on the rotating shaft; one end of the rotating shaft is rotatably connected with the base through a shaft sleeve arranged on the base, and the other end of the rotating shaft is connected with a bracket at the bottom of the rotary table;
the rotary table is of an annular structure, racks matched with the stepped gear shafts are arranged on the inner side wall of the rotary table along the circumferential direction, and the stepped gear shafts of the culture dish rotating mechanisms are uniformly distributed in the rotary table and are respectively in tooth joint with the racks on the inner side wall of the rotary table;
the regulator is a telescopic regulating frame with an inverted L-shaped structure and consists of a base rod, a first telescopic rod and a second telescopic rod; the base rod and the first telescopic rod are both of hollow structures, the base rod is arranged on the base, the first telescopic rod is sleeved inside the base rod, the second telescopic rod is sleeved inside the first telescopic rod, and the end part of the second telescopic rod is connected with the side wall of the annular supporting seat; the first telescopic rod is of an inverted L-shaped structure.
The invention has the following beneficial effects:
the invention drives a plurality of groups of cell culture dishes to rotate by a mode similar to that of driving a small turntable to rotate by a large turntable, thereby simulating the gravity environment. The invention can simulate the culture of a plurality of groups of cells under the gravity environment only by adopting one motor to drive, thereby greatly improving the efficiency.
The invention drives the rotating disc to rotate through the motor, a plurality of groups of rotating mechanisms provided with culture dishes are arranged at the opening at the middle part of the rotating disc, a stepped gear shaft of the rotating mechanism is mutually in toothed connection with a rack on the inner wall of the opening of the rotating disc, the culture dishes are arranged in a U-shaped seat fixedly connected with the stepped gear shaft, the U-shaped seat is positioned in an opening at the middle part of an annular supporting seat of the rotating mechanism, and the culture dishes are fixed through a binding band. And a rolling rod is arranged in the annular supporting seat of the rotating mechanism and is in rolling connection with the U-shaped seat. The U-shaped seat in every group slewing mechanism and the culture dish that is located the U-shaped seat can be driven to rotate when the carousel rotates, and the U-shaped seat rolls with the rod roller when rotating and is connected, and the rod roller is located the surround region that the rod roller standing groove of U-shaped seat and the rod roller standing groove on the annular supporting seat formed to block the U-shaped seat through the rod roller, prevent that slewing mechanism's step gear can break away from the carousel when high-speed the rotation, make it keep on the carousel rather than the meshing all the time. Thereby realize that annular supporting seat keeps motionless, only culture dish pivoted purpose. The annular supporting seat is connected with the base through the telescopic adjusting frame of the inverted L-shaped structure, and the adjustment of the horizontal direction and the vertical direction of the stepped gear shaft is realized through adjusting different adjusting rods of the telescopic adjusting frame. Therefore, the racks on the surfaces of the shafts with different diameters on the stepped gear shaft are in toothed connection with the racks on the inner wall of the turntable, so that the culture dish can rotate at different rotating speeds, and the conditions of different gravity can be simulated.
The device is also provided with an introducing device for gas required by cell culture, the introducing device is sleeved on the gas inlet pipeline through a sleeve, and a gamma type fixing rod connects the sleeve and the annular supporting seat together to achieve the purpose of fixing the sleeve. The air seal teeth are arranged between the sleeve and the air inlet pipeline, so that air can be prevented from leaking out as much as possible, and the annular support plate on the outer side of the air inlet pipeline is arranged in the groove at the bottom of the sleeve in a protruding mode, so that air is further prevented from leaking out, and sufficient air is guaranteed to be introduced. And the purposes that the air inlet pipeline rotates along with the culture dish and the cylinder sleeve does not rotate can be realized.
The invention can realize the simultaneous culture of different cells at the same rotating speed, also can realize the simultaneous culture of the same cells at different rotating speeds in different culture dishes by adjusting the position of the stepped gear shaft, and the like.
Drawings
FIG. 1 is a schematic view of the apparatus of the present invention;
FIG. 2 is a cross-sectional view of a rotating mechanism of the culture dish according to the present invention;
FIG. 3 is a cross-sectional view of the cartridge and air inlet duct of the present invention;
FIG. 4 is a schematic view of the connection between a gamma fixing rod and an annular support seat according to the present invention;
in the figure, 1 base, 2 turnplate, 3 regulator, 31 basic rod, 31 first telescopic rod, 33 second telescopic rod, 41 stepped gear shaft, 42 annular supporting seat, 43 rolling rod, 44 culture dish, 45U-shaped seat, 51 motor, 52 transmission gear I, 53 transmission gear II, 54 rotating shaft, 61 air inlet pipeline, 62 sleeve, 63 '+' type fixed rod, 64 groove, 65 gas seal tooth, 66 annular supporting plate, 67 external thread turnbuckle, 68 boss and 421 blind hole.
Detailed Description
It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples for carrying out the invention, and that various changes in form and details may be made therein without departing from the spirit and scope of the invention in practice.
To make the objects, aspects and advantages of the embodiments of the present invention more apparent, the following detailed description clearly illustrates the spirit of the disclosure, and any person skilled in the art, after understanding the embodiments of the disclosure, may make changes and modifications to the technology taught by the disclosure without departing from the spirit and scope of the disclosure.
The exemplary embodiments of the present invention and the description thereof are provided to explain the present invention and not to limit the present invention.
The first embodiment is as follows: the embodiment is described with reference to fig. 1 to 4, and the device for simulating gravity culture of cells in microgravity environment of the embodiment comprises a base 1, a rotating disc 2, a driving mechanism, a plurality of culture dish rotating mechanisms and a plurality of regulators 3;
the culture dish rotating mechanism consists of a stepped gear shaft 41, an annular supporting seat 42, a rolling rod 43 and a culture dish 44; the diameter of the stepped gear shaft 41 is gradually increased from bottom to top, a rack is arranged on the surface of the stepped gear shaft 41, and the top end of the stepped gear shaft 41 is connected with the bottom of the U-shaped seat 45; a roller holding groove is formed in the inner wall of the annular supporting seat 42 along the circumferential direction, and the roller 43 is arranged in the roller holding groove; the U-shaped seat 45 is arranged in the annular supporting seat 42, and the outer side wall of the U-shaped seat is in rolling connection with the roller 43; the culture dish 44 is placed in the U-shaped seat 45;
the driving mechanism consists of a motor 51, a first transmission gear 52, a second transmission gear 53 and a rotating shaft 54 which are arranged in the base; the first transmission gear 52 is arranged on a power output shaft of the motor 51 and is in tooth joint with a second transmission gear 53 arranged on a rotating shaft 54; one end of the rotating shaft 54 is rotatably connected with the base through a shaft sleeve arranged on the base, and the other end of the rotating shaft is connected with a support 7 at the bottom of the turntable 2;
the rotary table 2 is of an annular structure, racks matched with the stepped gear shafts 41 are arranged on the inner side wall of the rotary table 2 along the circumferential direction, and the stepped gear shafts 41 of the plurality of culture dish rotating mechanisms are uniformly distributed in the rotary table 2 and are respectively in tooth joint with the racks on the inner side wall of the rotary table 2;
the regulator 3 is a telescopic regulating frame with an inverted L-shaped structure, and the regulator 3 consists of a base rod 31, a first telescopic rod 32 and a second telescopic rod 33; the base rod 31 and the first telescopic rod 32 are both of a hollow structure, the base rod 31 is arranged on the base 1, the first telescopic rod 32 is sleeved inside the base rod 31, the second telescopic rod 33 is sleeved inside the first telescopic rod 32, and the end part of the second telescopic rod is connected with the side wall of the annular supporting seat 42; the first telescopic rod 32 is of an inverted L-shaped structure.
The second embodiment is as follows: the present embodiment will be described with reference to fig. 1 to 4, and the present embodiment is different from the specific embodiment in that: the culture dish rotating mechanism is provided with a gas input mechanism, and the gas input mechanism consists of a gas inlet pipeline 61, a sleeve 62 and a gamma type fixing rod 63; the air inlet pipeline 61 is communicated with an air inlet at the upper part of the culture dish 44, an annular groove 64 is formed in the side wall of the bottom of the sleeve 62, and an air seal tooth 65 is arranged on the inner side wall of the bottom; the air inlet pipe 61 is circumferentially provided with an annular support plate 66, the annular support plate 66 is circumferentially provided with a protrusion, and the protrusion is arranged in the annular groove 64; one end of a gamma-type fixing rod 63 is connected with the outer side wall of the sleeve 62, and the other end of the gamma-type fixing rod is connected with the top of the annular supporting seat 42; the top opening of the sleeve 62 is communicated with the air inlet of the air inlet pipe.
The rest is the same as the first embodiment.
The third concrete implementation mode: the present embodiment will be described with reference to fig. 1 to 4, and the present embodiment is different from the present embodiment in that: one end of a gamma-type fixing rod 63 is connected with the outer side wall of the sleeve 62, and the other end of the gamma-type fixing rod is connected with the top of the annular supporting seat 42; the top of the annular supporting seat 42 is provided with a blind hole 421, and the inner side wall of the blind hole 421 is provided with an internal thread; the external thread turnbuckle 67 is sleeved on the gamma type fixing rod 63, the outer edge of the end part of the gamma type fixing rod 63 connected with the annular supporting seat 42 extends outwards to form a boss 68 and is arranged in the blind hole 421, and the external thread turnbuckle 67 sleeved on the gamma type fixing rod 63 is in threaded connection with the internal thread of the inner side wall of the blind hole 421.
The rest is the same as the first embodiment.
The fourth concrete implementation mode: the present embodiment will be described with reference to fig. 1 to 4, and the present embodiment is different from the specific embodiment in that: the bottom of the external thread screw sleeve 67 is abutted against the top of the boss 68.
The rest is the same as the first embodiment.
The fifth concrete implementation mode: the present embodiment will be described with reference to fig. 1 to 4, and the present embodiment is different from the specific embodiment in that: the gas input by the gas input mechanism is carbon dioxide or oxygen.
The rest is the same as the first embodiment.
The sixth specific implementation mode: the present embodiment will be described with reference to fig. 1 to 4, and the present embodiment is different from the specific embodiment in that: the culture dish 44 is fixed by an elastic band arranged on the top of the U-shaped seat 45.
The rest is the same as the first embodiment.
The seventh embodiment: the present embodiment will be described with reference to fig. 1 to 4, and the present embodiment is different from the specific embodiment in that: the roller 43 is fixed in the roller placement groove by a retainer.
The rest is the same as the first embodiment.
The specific implementation mode is eight: the present embodiment will be described with reference to fig. 1 to 4, and the present embodiment is different from the specific embodiment in that: the first telescopic rod 32 is fixed by a limit bolt arranged on the base rod 31, and the second telescopic rod 33 is fixed by a limit bolt arranged on the first telescopic rod 32.
Other components and connection modes are the same as those of the first embodiment.
The specific implementation method nine: the present embodiment will be described with reference to fig. 1 to 4, and the present embodiment is different from the present embodiment in that: and the inner wall of the U-shaped seat 45 is provided with an anti-slip pad along the circumferential direction.
The rest is the same as the first embodiment.
The detailed implementation mode is ten: the present embodiment will be described with reference to fig. 1 to 4, and the present embodiment is different from the specific embodiment in that: and a second roller placing groove matched with the roller placing groove is formed in the outer side wall of the U-shaped seat 45, and the roller 43 is positioned in a surrounding area formed by the second roller placing groove and the roller placing groove.
Other components and connection modes are the same as those of the first embodiment.
The invention is not limited to the above embodiments, and one or a combination of several embodiments may also achieve the object of the invention.
Example 1
The device for simulating gravity culture of cells in the microgravity environment comprises a base 1, a turntable 2, a driving mechanism, a plurality of culture dish rotating mechanisms and a plurality of regulators 3;
the culture dish rotating mechanism consists of a stepped gear shaft 41, an annular supporting seat 42, a rolling rod 43 and a culture dish 44; the diameter of the stepped gear shaft 41 is gradually increased from bottom to top, a rack is arranged on the surface of the stepped gear shaft 41, and the top end of the stepped gear shaft 41 is connected with the bottom of the U-shaped seat 45; a roller holding groove is formed in the inner wall of the annular supporting seat 42 along the circumferential direction, and the roller 43 is arranged in the roller holding groove; the U-shaped seat 45 is arranged in the annular supporting seat 42, and the outer side wall of the U-shaped seat is in rolling connection with the roller 43; the culture dish 44 is placed in the U-shaped seat 45;
the driving mechanism consists of a motor 51, a first transmission gear 52, a second transmission gear 53 and a rotating shaft 54 which are arranged in the base; the first transmission gear 52 is arranged on a power output shaft of the motor 51 and is in tooth joint with a second transmission gear 53 arranged on a rotating shaft 54; one end of the rotating shaft 54 is rotatably connected with the base through a shaft sleeve arranged on the base, and the other end of the rotating shaft is connected with a support 7 at the bottom of the turntable 2;
the rotary table 2 is of an annular structure, racks matched with the stepped gear shafts 41 are arranged on the inner side wall of the rotary table 2 along the circumferential direction, and the stepped gear shafts 41 of the plurality of culture dish rotating mechanisms are uniformly distributed in the rotary table 2 and are respectively in tooth joint with the racks on the inner side wall of the rotary table 2;
the regulator 3 is a telescopic regulating frame with an inverted L-shaped structure, and the regulator 3 consists of a base rod 31, a first telescopic rod 32 and a second telescopic rod 33; the base rod 31 and the first telescopic rod 32 are both of a hollow structure, the base rod 31 is arranged on the base 1, the first telescopic rod 32 is sleeved inside the base rod 31, the second telescopic rod 33 is sleeved inside the first telescopic rod 32, and the end part of the second telescopic rod is connected with the side wall of the annular supporting seat 42; the first telescopic rod 32 is of an inverted L-shaped structure.
The culture dish rotating mechanism is provided with a gas input mechanism, and the gas input mechanism consists of a gas inlet pipeline 61, a sleeve 62 and a gamma type fixing rod 63; the air inlet pipeline 61 is communicated with an air inlet at the upper part of the culture dish 44, an annular groove 64 is formed in the side wall of the bottom of the sleeve 62, and an air seal tooth 65 is arranged on the inner side wall of the bottom; the air inlet pipe 61 is circumferentially provided with an annular support plate 66, the annular support plate 66 is circumferentially provided with a protrusion, and the protrusion is arranged in the annular groove 64; one end of a gamma-type fixing rod 63 is connected with the outer side wall of the sleeve 62, and the other end of the gamma-type fixing rod is connected with the top of the annular supporting seat 42; the top opening of the sleeve 62 is communicated with the air inlet of the air inlet pipe.
This embodiment is realized by supplying CO during culturing stem cells2The gas can be replenished without stopping the machine.
One end of a gamma-type fixing rod 63 is connected with the outer side wall of the sleeve 62, and the other end of the gamma-type fixing rod is connected with the top of the annular supporting seat 42; the top of the annular supporting seat 42 is provided with a blind hole 421, and the inner side wall of the blind hole 421 is provided with an internal thread; the external thread turnbuckle 67 is sleeved on the gamma type fixing rod 63, the outer edge of the end part of the gamma type fixing rod 63 connected with the annular supporting seat 42 extends outwards to form a boss 68 and is arranged in the blind hole 421, and the external thread turnbuckle 67 sleeved on the gamma type fixing rod 63 is in threaded connection with the internal thread of the inner side wall of the blind hole 421. The bottom of the external thread screw sleeve 67 is abutted against the top of the boss 68 so as to fix the boss 68, and the gamma type fixing rod 63 is prevented from being separated from the blind hole 421. The inner wall of the U-shaped seat 45 is provided with the anti-slip pad along the circumferential direction, so that the culture dish can be prevented from moving in the U-shaped seat 45, and the culture dish is fixed. The outer side wall of the U-shaped seat 45 is provided with a second roller placing groove matched with the roller placing groove, the roller 43 is located in a surrounding area formed by the second roller placing groove and the roller placing groove, and the stepped gear shaft 41 of the rotating mechanism is prevented from being separated from the rack of the turntable 2 when the rotation is told through the limiting effect of the roller 43, so that the stepped gear shaft is always in tooth joint with the rack of the turntable 2.
The embodiment can realize that different cells are simultaneously cultivateed under the same rotational speed, also can realize contrasting in different culture dishes through the position of adjusting the step gear axle, and multiple circumstances such as the same cell is simultaneously cultivateed under different rotational speeds, and simple structure can realize multiple functions, has practical application and worth.

Claims (10)

1. A device suitable for simulating gravity culture cells in a microgravity environment is characterized by comprising a base (1), a turntable (2), a driving mechanism, a plurality of culture dish rotating mechanisms and a plurality of regulators (3);
the culture dish rotating mechanism consists of a stepped gear shaft (41), an annular supporting seat (42), a rolling rod (43) and a culture dish (44); the diameter of the stepped gear shaft (41) is gradually increased from bottom to top, a rack is arranged on the surface of the stepped gear shaft, and the top end of the stepped gear shaft (41) is connected with the bottom of the U-shaped seat (45); the inner wall of the annular supporting seat (42) is circumferentially provided with a roller placing groove, and the roller (43) is placed in the roller placing groove; the U-shaped seat (45) is arranged in the annular supporting seat (42), and the outer side wall of the U-shaped seat is in rolling connection with the rolling rod (43); the culture dish (44) is placed in the U-shaped seat (45);
the driving mechanism consists of a motor (51), a first transmission gear (52), a second transmission gear (53) and a rotating shaft (54) which are arranged in the base; the first transmission gear (52) is arranged on a power output shaft of the motor (51) and is in tooth joint with the second transmission gear (53) arranged on the rotating shaft (54); one end of the rotating shaft (54) is rotatably connected with the base through a shaft sleeve arranged on the base, and the other end of the rotating shaft is connected with a support (7) at the bottom of the turntable (2);
the rotary table (2) is of an annular structure, racks matched with the stepped gear shafts (41) are arranged on the inner side wall of the rotary table (2) along the circumferential direction, and the stepped gear shafts (41) of the culture dish rotating mechanisms are uniformly distributed in the rotary table (2) and are respectively in tooth joint with the racks on the inner side wall of the rotary table (2);
the adjuster (3) is a telescopic adjusting frame with an inverted L-shaped structure, and the adjuster (3) consists of a base rod (31), a first telescopic rod (32) and a second telescopic rod (33); the base rod (31) and the first telescopic rod (32) are both of a hollow structure, the base rod (31) is arranged on the base (1), the first telescopic rod (32) is sleeved inside the base rod (31), the second telescopic rod (33) is sleeved inside the first telescopic rod (32), and the end part of the second telescopic rod is connected with the side wall of the annular supporting seat (42); the first telescopic rod (32) is of an inverted L-shaped structure.
2. The device for simulating gravity cell culture in microgravity environment according to claim 1, wherein the culture dish rotating mechanism is provided with a gas input mechanism, and the gas input mechanism is composed of a gas inlet pipe (61), a sleeve (62) and a fixing rod (63) of a gamma type; the air inlet pipeline (61) is communicated with an air inlet at the upper part of the culture dish (44), an annular groove (64) is formed in the side wall of the bottom of the sleeve (62), and an air seal tooth (65) is arranged on the inner side wall of the bottom; the air inlet pipeline (61) is provided with an annular support plate (66) along the circumferential direction, the annular support plate (66) is provided with a bulge along the circumferential direction, and the bulge is arranged in the annular groove (64); one end of the gamma-type fixing rod (63) is connected with the outer side wall of the sleeve (62), and the other end of the gamma-type fixing rod is connected with the top of the annular supporting seat (42); the top opening of the sleeve (62) is communicated with the air inlet of the air inlet pipe.
3. The device for simulating gravity cell culture in microgravity environment according to claim 2, wherein the gamma fixing rod (63) has one end connected to the outer sidewall of the sleeve (62) and the other end connected to the top of the annular support base (42); the top of the annular supporting seat (42) is provided with a blind hole (421), and the inner side wall of the blind hole (421) is provided with an internal thread; the external thread turnbuckle (67) is sleeved on the gamma type fixing rod (63), the end part of the gamma type fixing rod (63) connected with the annular supporting seat (42) extends outwards to form a boss (68) and is arranged in the blind hole (421), and the external thread turnbuckle (67) sleeved on the gamma type fixing rod (63) is in threaded connection with the internal thread of the inner side wall of the blind hole (421).
4. The device for simulating gravity cell culture in microgravity environment according to claim 3, wherein the bottom of the externally threaded screw sleeve (67) abuts against the top of the boss (68).
5. The device according to claim 2, wherein the gas input mechanism inputs carbon dioxide or oxygen.
6. The device for simulating gravity cell culture in microgravity environment according to claim 1, wherein the culture dish (44) is fixed by an elastic band installed on the top of the U-shaped seat (45).
7. The device for simulating gravity culture of cells under microgravity environment according to claim 1, wherein the roller (43) is fixed in the roller placement groove by a retainer.
8. The device for simulating gravity cell culture in microgravity environment according to claim 1, wherein the first telescopic rod (32) is fixed by a limit bolt installed on the base rod (31), and the second telescopic rod (33) is fixed by a limit bolt installed on the first telescopic rod (32).
9. The device for simulating gravity cell culture in microgravity environment according to claim 1, wherein the inner wall of the U-shaped seat (45) is circumferentially provided with a non-slip mat.
10. The device for simulating gravity cell culture under microgravity environment according to claim 1, wherein the outer side wall of the U-shaped seat (45) is provided with a second roller holding groove matched with the roller holding groove, and the roller (43) is located in the enclosed area formed by the second roller holding groove and the roller holding groove.
CN202210474817.7A 2022-04-29 2022-04-29 Device suitable for simulating gravity culture cells under microgravity environment Withdrawn CN114672418A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115316067A (en) * 2022-07-18 2022-11-11 广州市超有机循环农业研究院 Breeding method for improving rice variety characteristics through microgravity and application of breeding method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115316067A (en) * 2022-07-18 2022-11-11 广州市超有机循环农业研究院 Breeding method for improving rice variety characteristics through microgravity and application of breeding method

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