CN114672415B

CN114672415B - Diluting device

Info

Publication number: CN114672415B
Application number: CN202210367633.0A
Authority: CN
Inventors: 谢建敏
Original assignee: Hubei Mingde Health Technology Co ltd
Current assignee: Hubei Mingde Health Technology Co ltd
Priority date: 2022-04-08
Filing date: 2022-04-08
Publication date: 2022-12-20
Anticipated expiration: 2042-04-08
Also published as: CN114672415A

Abstract

The invention discloses a diluting device, and relates to the technical field of dilution coating; in order to improve the reliability and convenience of dilution operation; the device comprises a base, wherein a diluting mechanism is arranged on the base and comprises a supporting component and a plurality of diluting components, and the diluting components are arranged on the supporting component; the dilution assembly comprises: a first dilution section of thick bamboo, a first dilution section of thick bamboo one side outer wall is provided with the scale mark, and detachable installs the ejector pin on the first dilution section of thick bamboo at top. The method is simple to operate, multiple measurement is not needed in the dilution process, the accuracy of dilution multiple is guaranteed, external air contact is avoided, other bacterial colonies are effectively prevented from being mixed, and the reliability is improved.

Description

Diluting device

Technical Field

The invention relates to the technical field of dilution coating, in particular to a dilution device.

Background

During the dilution operation, need take repeatedly and place in new diluent to reach appointed dilution concentration, though can satisfy certain user demand, the complex operation process is inconvenient, and sneaks into other bacterial colonies in the air easily, consequently, still remains to be improved.

Through retrieval, chinese patent application No. CN202020509125.8 discloses an inoculating loop with a diluted bacteria liquid groove, wherein the inoculating loop is made of integrally formed glass, the inoculating loop is provided with a handle, a ring and a ring rod arranged between the handle and the ring, one end of the ring rod is connected to the outer diameter of one side of the ring, a bacteria liquid dilution shallow groove circumferentially distributed on the outer diameter surface of the ring is arranged at the other side of the ring and at the position 1/2 of the thickness of the ring, the bacteria liquid dilution shallow groove spans across the half circumferential surface of the other side of the ring, the bacteria liquid dilution shallow groove is provided with two first side walls parallel to the end surface of the ring and two second side walls perpendicular to the end surface of the ring, the first side walls and the second side walls are connected through smooth chamfers, and the second side walls and the outer diameter of the ring are mutually perpendicular to each other, and the first side walls and the bottom surfaces of the bacteria liquid dilution shallow groove are mutually perpendicular to each other. The inoculating loop in the above patent has the following disadvantages: although certain use requirements can be met, the dilution operation is inconvenient, the dilution rate cannot be controlled accurately easily, and other bacterial colonies in the air are mixed easily, so that the improvement is needed.

Disclosure of Invention

The invention aims to solve the defects in the prior art and provides a diluting device.

In order to achieve the purpose, the invention adopts the following technical scheme:

a dilution device comprises a base, wherein a dilution mechanism is arranged on the base and comprises a support component and a plurality of dilution components, and the dilution components are arranged on the support component; the dilution assembly comprises:

the outer wall of one side of the first dilution cylinder is provided with scale marks, and the top of the first dilution cylinder is detachably provided with an ejector rod;

the first quantitative conveying chambers are respectively arranged at the bottom of the first dilution cylinder, and are alternately distributed with the first dilution cylinder; the upper and lower outer walls of the first quantitative conveying chamber are provided with through holes, and the interior of the first quantitative conveying chamber is communicated with the interiors of the two connected first dilution barrels through the upper and lower through holes;

the first conduction control part comprises a rotating disc and a separating block, the rotating disc is fixed at the central position of one side of the separating block, and the rotating disc is rotatably arranged at one side of the first quantitative conveying chamber; a sealing rubber pad is fixed on the outer wall of the separation block, and the separation block rotates on the inner wall of the first quantitative conveying chamber in a sealing mode through the sealing rubber pad; the separating block is of a butterfly structure matched with the first quantitative conveying chamber, and two symmetrical quantitative conveying cavities are formed between the separating block and the inner wall of the first quantitative conveying chamber.

Preferably: the dilution assembly further comprises:

the connecting chamber is arranged below the first quantitative conveying chamber at the bottommost part and is communicated with the first quantitative conveying chamber through the through hole;

the structure of the second dilution cylinder is the same as that of the first dilution cylinder, and the second dilution cylinder at the lowest part is communicated with the connecting chamber through a connecting pipe;

the structure of the second quantitative conveying chamber is the same as that of the first quantitative conveying chamber, the second quantitative conveying chamber is arranged above the second dilution cylinder, and the second quantitative conveying chamber and the second dilution cylinder are alternately distributed;

and the second conduction control part is arranged at the second quantitative conveying chamber and has the same structure as the first conduction control part.

Further: the support assembly includes:

the first rotating seat is rotatably arranged on the base;

the support frame is fixed at the center of the top of the first rotating seat;

the cross rods are fixed on the outer walls of the two sides of the top end of the support frame;

the fixing frame is rotatably arranged on the outer wall of the cross rod, and a first diluting cylinder and a second diluting cylinder are respectively fixed on the inner walls of the two ends of the fixing frame;

and the fixing piece is arranged on the fixing frame and used for fixing the fixing frame on the cross rod and limiting the angle of the fixing frame.

Further preferred is: the fixing member includes:

the fixing pin, the cross bar and the fixing frame are provided with pin holes, and the fixing pin is detachably inserted in the pin holes;

two ends of the spring are respectively fixed at one end of the fixed pin and the outer wall of one side of the fixed frame;

and the pull ring is fixed at the top end of the fixing pin.

As a preferable aspect of the present invention: the outer wall of one side of first dilution section of thick bamboo and second dilution section of thick bamboo all is provided with leads to the liquid pipe, it is connected with the valve body to lead to the liquid intraductal rotation, and valve body one end is fixed with control knob.

Further preferred according to the invention are: a glue film is fixed on the inner wall of one end of the liquid through pipe, a micro-seam is formed on the glue film, and the micro-seam is kept in a closed state under the condition that the glue film is not deformed; lead to liquid pipe cooperation and use and have L type intubate, L type intubate top is provided with the liquid feeding fill, and L type intubate detachable pegs graft in leading to the liquid intraductal.

As a still further scheme of the invention: install on the base and cultivate the mechanism, it includes to cultivate the mechanism:

the second rotating seat is rotatably arranged at the top of the base;

the culture chamber is fixed on the outer wall of the top of the second rotating seat;

and the culture dish is placed on the inner side of the culture room.

On the basis of the scheme: and a uniformly distributed check curtain is fixed on the outer wall of one side of the culture chamber.

On the basis of the foregoing scheme, it is preferable that: a temperature control assembly is fixed on the inner wall of one side of the culture chamber.

A dilution and coating method comprising the steps of:

s1: sampling a bacterial liquid sample, wherein the using amount is equal to 1 time of the volume of the quantitative conveying cavity;

s2: injecting diluent into the first diluting cylinder and the second diluting cylinder, wherein the injection amount of the diluent is 9 times of the volume of the quantitative conveying cavity;

s3: injecting sample bacteria liquid into the uppermost first dilution cylinder;

s4: after fully mixing, screwing a rotating disc below the first dilution cylinder, and discharging the diluted bacteria liquid in the first dilution cylinder into the next first dilution cylinder through a quantitative conveying cavity, wherein the discharge amount is the volume of the quantitative conveying cavity;

s5: repeating the steps until the dilution reaches the designated multiple;

s6: taking out the diluted bacteria liquid from the liquid through pipe;

s7: after the coater is sterilized, dipping the taken bacterial liquid;

s8: the bacterial liquid is coated on a culture medium with a culture dish by using a coater for culture.

The invention has the beneficial effects that:

1. the invention can inject the diluent into each first diluting cylinder when diluting, because the volume of the quantitative conveying cavity is a fixed value, the diluent injected into the first diluting cylinder is controlled to be a fixed multiple of the volume of the quantitative conveying cavity, for example, when 9 times, the rotating disc is rotated to drive the separating block to rotate, the quantitative conveying cavity is firstly communicated with the first diluting cylinder above, after the liquid above enters and fills the quantitative conveying cavity, the rotating disc is rotated to ensure that the quantitative conveying cavity is communicated with the first diluting cylinder below, the liquid in the quantitative conveying cavity is discharged into the first diluting cylinder below to be mixed with the diluent with corresponding multiple, thereby achieving the purpose of diluting, the steps are repeated until the dilution is diluted to the designated multiple, the mode is simple to operate, measurement is not needed for multiple times in the dilution process, the accuracy of the dilution multiple is guaranteed, the external air is not contacted, the rest of liquid is effectively prevented from being mixed, and the reliability is improved.

2. By arranging the second dilution cylinder, the second quantitative conveying chamber and other structures, the dilutable multiple can be improved, the condition that the overall structure is overlong is avoided, the space is saved, and the practicability is improved; through setting up mount, horizontal pole isotructure, the rotation that can be convenient dilutes the subassembly to do benefit to and reverse the upper and lower both ends of a first dilution section of thick bamboo and a second dilution section of thick bamboo, thereby be convenient for control its inside liquid and flow according to the assigned direction, promoted the practicality.

3. Through setting up fixed pin, spring isotructure, can insert the fixed pin in the pinhole according to the demand to carry on spacingly to the angle that dilutes the subassembly, when the subassembly is diluted in needs upset, only need with the fixed pin extract from the pinhole, with the mount rotatory 180 back again with the fixed pin insert in the pinhole can, promoted the practicality.

4. By arranging the control knob, the liquid through pipe and other structures, liquid can be conveniently added and taken from the liquid through pipe, and the practicability is improved; through setting up liquid feeding fill, L type intubate and glued membrane isotructure, can insert L type intubate in the slit of glued membrane according to the demand, fight the liquid feeding and switch on with logical liquid pipe mutually, and then can be convenient fight from the liquid feeding and locate the liquid feeding, promoted the practicality.

Drawings

FIG. 1 is a schematic structural diagram of a dilution apparatus according to the present invention;

FIG. 2 is a schematic sectional view of a culture chamber of a dilution device according to the present invention;

FIG. 3 is a schematic structural diagram of a dilution mechanism of a dilution device according to the present invention;

FIG. 4 is a schematic view of a dilution unit of the dilution apparatus according to the present invention;

FIG. 5 is a schematic cross-sectional structural diagram of a first diluting cylinder and a first quantitative conveying chamber of a diluting device according to the present invention;

FIG. 6 is a schematic sectional view of a dilution device according to the present invention, after the L-shaped insertion tube and the filling tube are detached from each other;

fig. 7 is a schematic cross-sectional structural view of a fixing frame of a dilution apparatus according to the present invention.

In the figure: the automatic quantitative culture device comprises a base 1, a first rotating seat 2, a support frame 3, a first diluting cylinder 4, a first quantitative conveying chamber 5, a liquid adding hopper 6, a culture chamber 7, a retaining curtain 8, a second rotating seat 9, a rotating disc 10, a temperature control assembly 11, a culture dish 12, a cross rod 13, a top rod 14, a port 15, a second quantitative conveying chamber 16, a connecting pipe 17, a connecting chamber 18, a control knob 19, a scale mark 20, a second diluting cylinder 21, a fixing frame 22, a sealing rubber pad 23, a separation block 24, a quantitative conveying cavity 25, a 26L-shaped insertion pipe, a liquid through pipe 27, a 28 micro-seam, a valve body 29, a rubber film 30, a pin hole 31, a fixing pin 32, a spring 33 and a pull ring 34.

Detailed Description

The technical solution of the present patent will be further described in detail with reference to the following embodiments.

Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.

In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.

In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by one of ordinary skill in the art as appropriate.

Example 1:

a dilution device, as shown in fig. 1-7, comprising a base 1, wherein a dilution mechanism is mounted on the base 1, the dilution mechanism comprises a support component and a plurality of dilution components, and the dilution components are mounted on the support component; the dilution assembly comprises:

the outer wall of one side of the first diluting cylinder 4 is provided with scale marks 20, and the top part of the first diluting cylinder 4 is detachably provided with an ejector rod 14;

the first quantitative conveying chambers 5 are respectively and integrally arranged at the bottom of the first dilution cylinder 4, and the first quantitative conveying chambers 5 and the first dilution cylinder 4 are alternately distributed; the upper outer wall and the lower outer wall of the first quantitative conveying chamber 5 are provided with through holes 15, and the inside of the first quantitative conveying chamber 5 is communicated with the insides of the two connected first dilution cylinders 4 through the upper through hole 15 and the lower through hole 15;

the first conduction control part comprises a rotating disc 10 and a separating block 24, the rotating disc 10 is fixed at the central position of one side of the separating block 24, and the rotating disc 10 is rotatably arranged at one side of the first quantitative conveying chamber 5; the outer wall of the separation block 24 is bonded with a sealing rubber gasket 23, and the separation block 24 rotates on the inner wall of the first quantitative conveying chamber 5 in a sealing way through the sealing rubber gasket 23; the separating block 24 is in a butterfly structure matched with the first quantitative conveying chamber 5, and two symmetrical quantitative conveying cavities 25 are formed between the separating block 24 and the inner wall of the first quantitative conveying chamber 5; the rotating disc 10 can drive the separating block 24 to rotate, so that the position of the quantitative conveying cavity 25 is changed, and the quantitative conveying cavity can be communicated with the first diluting cylinder 4 at the top or the first diluting cylinder 4 at the bottom;

through the arrangement of the first dilution cylinder 4, the first quantitative conveying chamber 5, the partition block 24 and other structures, when dilution treatment is performed, diluent can be injected into each first dilution cylinder 4, and because the volume of the quantitative conveying cavity 25 is a fixed value, the diluent injected into the first dilution cylinder 4 is controlled to be a fixed multiple of the volume of the quantitative conveying cavity 25, if 9 times, the rotating disc 10 is rotated to drive the partition block 24 to rotate, the quantitative conveying cavity 25 is firstly communicated with the first dilution cylinder 4 above, after the liquid above enters and fills the quantitative conveying cavity 25, the rotating disc 10 is rotated to enable the quantitative conveying cavity 25 to be communicated with the first dilution cylinder 4 below, the liquid in the quantitative conveying cavity 25 is discharged into the first dilution cylinder 4 below and is mixed with the diluent of the corresponding multiple, so that the dilution purpose is achieved, the steps are repeated until the dilution is performed to the specified multiple, the method is simple to operate, multiple measurement is not needed in the dilution process, the accuracy of the dilution multiple is guaranteed, external air contact is avoided, the mixing of other bacterial colonies is effectively prevented, and the reliability is improved.

For better spatial layout; as shown in fig. 3 and 4, the dilution unit further includes:

a connection chamber 18, wherein the connection chamber 18 is integrally arranged below the first quantitative conveying chamber 5 at the bottommost part, and the connection chamber 18 is communicated with the first quantitative conveying chamber 5 through the through opening 15;

a second dilution cylinder 21, the structure of the second dilution cylinder 21 is the same as that of the first dilution cylinder 4, and the second dilution cylinder 21 at the lowest part is communicated with the connecting chamber 18 through a connecting pipe 17;

the structure of the second quantitative conveying chamber 16 is the same as that of the first quantitative conveying chamber 5, the second quantitative conveying chamber 16 is integrally arranged above the second dilution cylinder 21, and the second quantitative conveying chamber 16 and the second dilution cylinder 21 are alternately distributed;

a second conduction control part which is arranged at the second quantitative conveying chamber 16 and has the same structure with the first conduction control part;

through setting up the second and diluting a section of

thick bamboo

21, 16 isotructures of second ration delivery chamber, can promote the multiple that can dilute, and avoided the condition of overall structure overlength, practiced thrift the space, promoted the practicality.

For convenience of operation; as shown in fig. 3 and 4, the support assembly includes:

the first rotating seat 2, the first rotating seat 2 can be rotatably arranged on the base 1;

the support frame 3 is fixed at the center of the top of the first rotating seat 2 through screws;

the cross rods 13 are fixed on the outer walls of the two sides of the top end of the support frame 3;

the fixing frame 22 is rotatably arranged on the outer wall of the cross rod 13, and the first diluting cylinder 4 and the second diluting cylinder 21 are respectively fixed on the inner walls of two ends of the fixing frame 22;

the fixing piece is arranged on the fixing frame 22 and used for fixing the fixing frame 22 on the cross rod 13 and limiting the angle of the fixing frame 22;

through setting up structures such as mount 22, horizontal pole 13, the rotation that can be convenient dilutes the subassembly to do benefit to and reverse the upper and lower both ends of first dilution section of thick bamboo 4 and second dilution section of thick bamboo 21, thereby be convenient for control its inside liquid and flow according to the assigned direction, promoted the practicality.

To facilitate securing the mount 22; as shown in fig. 7, the fixing member includes:

the fixing pin 32, the cross bar 13 and the fixing frame 22 are provided with pin holes 31, and the fixing pin 32 is detachably inserted in the pin holes 31;

a spring 33, wherein two ends of the spring 33 are respectively fixed on one end of the fixing pin 32 and one side outer wall of the fixing frame 22;

a pull ring 34, wherein the pull ring 34 is fixed at the top end of the fixed pin 32;

through setting up the fixed pin 32, spring 33 isotructure, can be as required, insert the fixed pin 32 in the pinhole 31 to the angle to diluting the subassembly carries on spacingly, when the subassembly is diluted in needs upset, only need extract the fixed pin 32 from the pinhole 31 in, with the rotatory 180 back of mount 22 again with the fixed pin 32 insert in the pinhole 31 can, promoted the practicality.

The liquid is convenient to add and take; as shown in fig. 4-6, a liquid flowing pipe 27 is arranged on the outer wall of one side of each of the first diluting cylinder 4 and the second diluting cylinder 21, a valve body 29 is rotatably connected to the liquid flowing pipe 27, and a control knob 19 is fixed at one end of the valve body 29; through setting up structures such as control knob 19, liquid pipe 27, can be convenient get liquid from liquid pipe 27 liquid feeding, promoted the practicality.

In order to more conveniently add and take liquid; as shown in fig. 5 and 6, a glue film 30 is adhered to an inner wall of one end of the liquid through pipe 27, a micro-slit 28 is formed on the glue film 30, and the micro-slit 28 is kept in a sealed state under the condition that the glue film 30 is not deformed; the liquid passing pipe 27 is matched with an L-shaped insertion pipe 26, the top end of the L-shaped insertion pipe 26 is integrally provided with a liquid adding hopper 6, and the L-shaped insertion pipe 26 is detachably inserted into the liquid passing pipe 27; through setting up liquid feeding fill 6, L type intubate 26 and glued membrane 30 isotructure, can insert L type intubate 26 in glued membrane 30's crack 28, fight liquid feeding 6 and lead to liquid pipe 27 and switch on mutually according to the demand, and then can be convenient fight 6 liquid feeding from the liquid feeding, promoted the practicality.

Example 2:

a dilution device, as shown in FIGS. 1 and 2, for facilitating cultivation; the present embodiment is modified from embodiment 1 in the following way: install on the base 1 and cultivate the mechanism, it includes to cultivate the mechanism:

the second rotating seat 9, the second rotating seat 9 can be rotatably installed on the top of the base 1;

the culture chamber 7 is fixed on the outer wall of the top of the second rotating seat 9;

a culture dish 12, the culture dish 12 being placed inside the culture chamber 7.

In order to improve the practicability; as shown in fig. 1, the outer wall of one side of the culture chamber 7 is bonded with the uniformly distributed check curtains 8, and the inner wall of one side of the culture chamber 7 is fixed with the temperature control assembly 11 through screws; through setting up the check curtain 8, can place the pollution, for cultivateing providing the guarantee, set up accuse temperature subassembly 11 and can be convenient for cultivate the cultivation temperature in the room 7 to the accuse, promoted the practicality.

Example 3:

a dilution and coating method comprising the steps of:

s1: sampling bacterial liquid, wherein the using amount is equal to 1 time of the volume of the quantitative conveying cavity 25;

s2: injecting diluent into the first dilution cylinder 4 and the second dilution cylinder 21, wherein the injection amount of the diluent is 9 times of the volume of the quantitative conveying cavity 25;

s3: injecting sample bacteria liquid into the uppermost first dilution cylinder 4;

s4: after the mixture is fully mixed, the rotating disc 10 below the first dilution cylinder is screwed, and the diluted bacteria liquid in the first dilution cylinder 4 is discharged into the next first dilution cylinder 4 through the quantitative conveying cavity 25, wherein the discharge amount is the volume of the quantitative conveying cavity 25;

s5: repeating the steps until the dilution reaches the designated multiple;

s6: taking out the diluted bacteria liquid from the liquid through pipe 27;

s7: after the coater is sterilized, dipping the taken bacterial liquid;

s8: the culture medium with the petri dish 12 is coated with the bacterial liquid by using an applicator to culture.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. A diluting device comprises a base (1), and is characterized in that a diluting mechanism is mounted on the base (1), the diluting mechanism comprises a supporting component and a plurality of diluting components, and the diluting components are mounted on the supporting component; the dilution assembly comprises:

the device comprises a first dilution barrel (4), scale marks (20) are arranged on the outer wall of one side of the first dilution barrel (4), and a top rod (14) is detachably arranged on the first dilution barrel (4) at the top;

the first quantitative conveying chambers (5) are respectively arranged at the bottoms of the first dilution cylinders (4), and the first quantitative conveying chambers (5) and the first dilution cylinders (4) are alternately distributed; the upper outer wall and the lower outer wall of the first quantitative conveying chamber (5) are provided with through holes (15), and the interior of the first quantitative conveying chamber (5) is communicated with the interiors of two connected first diluting barrels (4) through the upper through hole and the lower through hole (15);

the first conduction control part comprises a rotating disc (10) and a separating block (24), the rotating disc (10) is fixed at the central position of one side of the separating block (24), and the rotating disc (10) is rotatably arranged at one side of the first quantitative conveying chamber (5); a sealing rubber gasket (23) is fixed on the outer wall of the separation block (24), and the separation block (24) rotates on the inner wall of the first quantitative conveying chamber (5) in a sealing mode through the sealing rubber gasket (23); the separating block (24) is in a butterfly structure matched with the first quantitative conveying chamber (5), and two symmetrical quantitative conveying cavities (25) are formed between the separating block (24) and the inner wall of the first quantitative conveying chamber (5);

the dilution assembly further comprises:

a connecting chamber (18), wherein the connecting chamber (18) is arranged below the first quantitative conveying chamber (5) at the bottommost part, and the connecting chamber (18) is communicated with the first quantitative conveying chamber (5) through a through opening (15);

the structure of the second dilution cylinder (21) is the same as that of the first dilution cylinder (4), and the second dilution cylinder (21) at the lowest part is communicated with the connecting chamber (18) through a connecting pipe (17);

the structure of the second quantitative conveying chamber (16) is the same as that of the first quantitative conveying chamber (5), the second quantitative conveying chamber (16) is arranged above the second dilution cylinder (21), and the second quantitative conveying chamber (16) and the second dilution cylinder (21) are arranged in an alternating distribution manner;

a second conduction control part which is arranged at the second quantitative conveying chamber (16) and has the same structure with the first conduction control part;

the support assembly includes:

the first rotating seat (2), the first rotating seat (2) is rotatably arranged on the base (1);

the support frame (3), the support frame (3) is fixed in the center position of the top of the first rotating seat (2);

the cross rods (13), the cross rods (13) are fixed on the outer walls of the two sides of the top end of the support frame (3);

the fixing frame (22), the fixing frame (22) is rotatably arranged on the outer wall of the cross rod (13), and a first diluting cylinder (4) and a second diluting cylinder (21) are respectively fixed on the inner walls of the two ends of the fixing frame (22);

the fixing piece is arranged on the fixing frame (22) and used for fixing the fixing frame (22) on the cross rod (13) and limiting the angle of the fixing frame (22);

the fixing member includes:

the fixing pin (32), the cross rod (13) and the fixing frame (22) are provided with pin holes (31), and the fixing pin (32) is detachably inserted in the pin holes (31);

two ends of the spring (33) are respectively fixed at one end of the fixing pin (32) and the outer wall of one side of the fixing frame (22);

a pull ring (34), wherein the pull ring (34) is fixed at the top end of the fixed pin (32);

liquid passing pipes (27) are arranged on the outer walls of one sides of the first dilution cylinder (4) and the second dilution cylinder (21), a valve body (29) is connected in the liquid passing pipes (27) in a rotating mode, and a control knob (19) is fixed at one end of the valve body (29);

a glue film (30) is fixed on the inner wall of one end of the liquid through pipe (27), a micro-slit (28) is formed in the glue film (30), and the micro-slit (28) keeps a closed state under the condition that the glue film (30) is not deformed; the liquid passing pipe (27) is matched with an L-shaped insertion pipe (26), the top end of the L-shaped insertion pipe (26) is provided with a liquid adding hopper (6), and the L-shaped insertion pipe (26) is detachably inserted into the liquid passing pipe (27).

2. A dilution apparatus according to claim 1, wherein the base (1) has culture means mounted thereon, the culture means including:

the second rotating seat (9), the second rotating seat (9) is rotatably arranged at the top of the base (1);

the culture chamber (7), the culture chamber (7) is fixed on the outer wall of the top of the second rotating seat (9);

a culture dish (12), wherein the culture dish (12) is arranged inside the culture chamber (7).

3. A dilution apparatus according to claim 2, wherein the cultivation room (7) is provided with a uniformly distributed curtain (8) on one side of the outer wall.

4. A dilution apparatus according to claim 3, wherein a temperature control member (11) is fixed to an inner wall of the culture chamber (7).

5. A dilution and coating method for a dilution unit according to any one of claims 1 to 4, comprising the steps of:

s1: sampling bacterial liquid, wherein the taking amount is equal to 1 time of the volume of the quantitative conveying cavity (25);

s2: injecting a diluent into the first dilution cylinder (4) and the second dilution cylinder (21), wherein the injection amount of the diluent is 9 times of the volume of the quantitative conveying cavity (25);

s3: injecting sample bacteria liquid into the uppermost first dilution cylinder (4);

s4: after being fully mixed, the diluted bacterium liquid in the first dilution cylinder (4) is discharged into the next first dilution cylinder (4) through a quantitative conveying cavity (25), and the discharge amount is the volume of the quantitative conveying cavity (25);

s5: repeating the steps until the dilution reaches the designated multiple;

s6: taking out the diluted bacteria liquid from the liquid through pipe (27);

s7: after the coater is sterilized, dipping the taken bacterial liquid;

s8: the bacterial liquid is spread on a culture medium with a culture dish (12) by using a spreader to culture.