CN114669402A - Preparation equipment of umbilical cord-derived mesenchymal stem cell exosome oral repair spraying agent - Google Patents
Preparation equipment of umbilical cord-derived mesenchymal stem cell exosome oral repair spraying agent Download PDFInfo
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- CN114669402A CN114669402A CN202210453476.5A CN202210453476A CN114669402A CN 114669402 A CN114669402 A CN 114669402A CN 202210453476 A CN202210453476 A CN 202210453476A CN 114669402 A CN114669402 A CN 114669402A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
- B04B11/04—Periodical feeding or discharging; Control arrangements therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
- B04B15/06—Other accessories for centrifuges for cleaning bowls, filters, sieves, inserts, or the like
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B2005/0435—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles with adapters for centrifuge tubes or bags
Abstract
The invention discloses preparation equipment of an oral repair spraying agent of an umbilical cord source mesenchymal stem cell exosome, and provides a scheme which comprises a workbench, wherein the top of the workbench is fixedly connected with a first support plate, the top of the workbench is provided with a placing disc, the top of the workbench is provided with a circular groove, the inner wall of the bottom of the circular groove is fixedly connected with a cylinder, a clamping assembly for clamping the placing disc is arranged in the workbench, the top end of the cylinder is provided with an extraction assembly for extracting supernatant, and the clamping assembly can control the lifting of the extraction assembly. The convenience is brought to the later stage, and the separation and the supernatant extraction are carried out on a plurality of centrifuge tubes at one time.
Description
Technical Field
The invention relates to the technical field of exosome oral repair spraying agents, in particular to preparation equipment of an umbilical cord-derived mesenchymal stem cell exosome oral repair spraying agent.
Background
Oral ulcer, commonly known as "aphtha", is a common ulcerative injury to the oral mucosa, which is usually found in the inner labial, tongue abdomen, buccal mucosa, vestibular sulcus, soft palate, etc., where the mucosa lacks cutinization layer or has poor keratosis. Tongue ulcer refers to oral ulcer occurring on tongue and tongue abdomen. The oral ulcer has severe pain and obvious local burning pain during the attack, and serious patients can influence diet and speaking, thereby causing great inconvenience to daily life.
Mesenchymal stem cells are multifunctional self-renewing cells and have great application potential in regenerative medicine, and can be used in clinical trials of regenerative research, and can be derived from bone marrow, dental pulp, adipose tissue and wolton jelly. Originally, mechanisms related to their biological functions, such as homing, transdifferentiation, and immunomodulation, have been reported, however, recent studies have shown that paracrine action of mesenchymal stem cells plays an important role in repair and regeneration by secreting extracellular vesicles (exosomes).
Therefore, the mesenchymal stem cells can repair the oral cavity rapidly, and the mesenchymal stem cell exosome oral cavity repair spraying agent mainly comprises two parts: firstly, separating NK cells from peripheral blood, carrying out in-vitro amplification and activation, collecting NK cell culture supernatant, and carrying out exosome enrichment; secondly, based on the NK cell exosomes, a plurality of amino acids, antibacterial ingredients and the like for wound repair are added to prepare a spray or a film coating agent, and the wound repair is carried out through the effects of sterilization and disinfection of the exosomes, nutrition of damaged cells, inhibition of excessive inflammatory reaction, promotion of regeneration of damaged tissues and the like, so that the healing of the wound is accelerated.
But when the foreign body is drawed, at first need extract the supernatant of NK cell, but need the staff to put into centrifugal device one by one with the centrifuging tube that is equipped with the cultured cell when drawing the supernatant at present and centrifuge one by one, and need draw the inside supernatant of centrifuging tube after the centrifugation, consequently need the staff to operate repeatedly when drawing centrifugation and supernatant to the cell, seriously influence the extraction separation of supernatant, and draw the extraction test tube that needs to be renewed after finishing drawing the supernatant and be used for the extraction of later stage exosome, and then very big extravagant extraction test tube, in addition put into centrifugal device with the centrifuging tube, high-speed rotation leads to the centrifuging tube to rock and the breakage easily.
Disclosure of Invention
The invention aims to solve the problems that in the prior art, the extraction efficiency is low, repeated operation is required by workers, an extraction test tube is wasted, and a centrifugal tube is easy to break, and provides the preparation equipment for the oral repair spraying agent of the umbilical cord-derived mesenchymal stem cell exosome, which has a compact structure.
In order to achieve the purpose, the invention adopts the following technical scheme:
the preparation equipment of the umbilical cord source mesenchymal stem cell exosome oral repair spraying agent comprises a workbench, wherein the top of the workbench is fixedly connected with a first support plate, the top of the workbench is provided with a placing disc, the top of the workbench is provided with a circular groove, and the inner wall of the bottom of the circular groove is fixedly connected with a cylinder;
a clamping assembly for clamping the placing disc is arranged in the workbench;
the top end of the cylinder is provided with an extraction component for extracting supernatant, and the clamping component can control the extraction component to lift;
the outer wall of the cylinder is provided with a cleaning component for cleaning the extraction component.
Preferably, the centre gripping subassembly is including setting up the rotation groove at the workstation top, the bottom inner wall rotation of rotating the groove is connected with the pivot, the top fixedly connected with disc of pivot is placed the dish and is placed the top at the disc, be equipped with the shifting chute in the disc, the top of disc is equipped with two spouts that communicate with the shifting chute, it is connected with two-way lead screw to be the horizontal rotation in the shifting chute, the outer wall thread bush of two-way lead screw is equipped with two sliders, slider and the bottom inner wall sliding connection of shifting chute, two sliders are located the positive and negative screw thread section of two-way lead screw respectively, spout and fixedly connected with splint are run through on the top of slider, the fixed cover of outer wall of two-way lead screw is equipped with first bevel gear, be equipped with driving motor in the workstation, driving motor's output shaft and the bottom fixed connection of pivot.
Preferably, one side sliding connection that first support plate is close to and places the dish has the slide, the slide internal rotation is connected with the rolling disc, the bottom of rolling disc is equipped with a plurality of seal grooves, the top of placing the dish is equipped with a plurality of standing grooves corresponding with the seal groove, the centrifuging tube has been placed in the standing groove, the rolling disc internal thread runs through there is the screw rod, the bottom of screw rod is rotated and is run through the top inner wall and the fixedly connected with second bevel gear of shifting chute, and second bevel gear meshes with first bevel gear mutually.
Preferably, the extraction element includes the second extension board of fixed connection on the cylinder top, one side sliding connection that the second extension board is close to the dish of placing has the connecting plate, the empty core tray of opposite side fixedly connected with of connecting plate, be equipped with a plurality of extraction tubes that are linked together in the empty core tray, the fixed cover of outer wall of extraction tube is equipped with the filter screen, the top fixedly connected with diaphragm pump of second extension board, the fixed cover of inlet of diaphragm pump is equipped with the feed liquor pipe, and the other end and the empty core tray of feed liquor pipe are linked together, the fixed cover of liquid outlet of diaphragm pump is equipped with the drain pipe.
Preferably, clean subassembly is established including rotating the cover and is established the rotor plate at the cylinder outer wall, the rotor plate internal rotation runs through there is the axis of rotation, the first carousel of top fixedly connected with of axis of rotation, the top of first carousel is equipped with the washing case, the bottom fixedly connected with spur gear of axis of rotation, one side top fixedly connected with second carousel of first carousel is kept away from to the rotor plate.
Preferably, the bottom inner wall sliding connection of circular recess has the rack with straight-teeth gear engaged with, the top fixedly connected with fixed block of rack, one side fixedly connected with extension spring of second extension board is kept away from to the fixed block, and the other end of extension spring and one side inner wall fixed connection of circular recess, the fixed cover of outer wall of pivot is equipped with the dysmorphism wheel, the rack is kept away from the one end of second extension board and is slided and extend to in the rotation inslot and touch with the dysmorphism wheel.
Preferably, place the dish and all be equipped with the spacing groove in the one side of keeping away from each other, two and spacing groove matched with stoppers of the top fixedly connected with of second carousel can carry on spacingly to placing the dish through the cooperation of spacing groove and stopper, when preventing to place the dish and place on the second carousel, the later stage can't make in the accurate centrifuging tube that inserts of extraction tube.
Preferably, the top fixedly connected with slide bar of disc, and the top of slide bar slides and runs through the rolling disc, can play the guide effect to the rolling disc through the slide bar, avoids the rolling disc slope to appear.
Preferably, one side fixedly connected with connecting rod that first extension board was kept away from to the slide, and the other end and the hollow dish fixed connection of connecting rod can link together centre gripping subassembly and extraction subassembly through the connecting rod, make things convenient for later stage centre gripping subassembly control to extract the subassembly and go up and down.
Preferably, the one side inner wall that the circular slot kept away from each other all is equipped with the rectangular channel, sliding connection has the wedge in the rectangular channel, the one end fixedly connected with spring of second extension board is kept away from to the wedge, and one side inner wall fixed connection of the other end of spring and rectangular channel, one side that the second extension board was kept away from to the second carousel is equipped with the draw-in groove with wedge looks block, rotate the rotor plate, make the rotor plate rotate 180, the rotor plate drives the second carousel and places the dish and rotate, when rotating 180, the wedge promotes the wedge card under the spring action of spring and goes into in the draw-in groove, it is spacing to the second carousel, rotate the screw rod afterwards and make the rotor plate drive connecting rod and empty core plate move down, extraction tube and filter screen extend to respectively in the centrifuging tube, can make the centrifuging tube that is located on the second carousel align with the extraction tube.
Compared with the prior art, the invention provides preparation equipment of the oral repair spraying agent of the umbilical cord-derived mesenchymal stem cell exosome, and the preparation equipment has the following beneficial effects:
1. this fixed cover of outer wall of two-way lead screw of preparation equipment of navel cord source mesenchymal stem cell exosome oral cavity restoration nebula is equipped with first bevel gear, the outer wall thread bush of two-way lead screw is equipped with two sliders, and two sliders are located the positive and negative screw thread section of two-way lead screw respectively, the rolling disc internal thread runs through there is the screw rod, the bottom of screw rod extends to in the shifting chute and fixedly connected with second bevel gear, second bevel gear meshes with first bevel gear mutually, and the rolling disc can make slider and splint remove to the centre when moving down vertical tight centrifuging tube when rotating the screw rod, transversely presss from both sides tight the placing disc, accomplishes the clamp of two directions simultaneously and presss from both sides tightly, avoids the centrifuging tube to appear rocking and break when carrying out centrifugal motion.
2. This preparation equipment of umbilical cord source mesenchymal stem cell exosome oral cavity restoration nebula's preparation is equipped with a plurality of standing grooves in the centrifuging tube, be equipped with the centrifuging tube in the standing groove, the bottom of hollow dish is equipped with a plurality of extraction tubes that are linked together, can install a plurality of centrifuging tubes on placing the dish, and then the centrifugation of a plurality of centrifuging tubes can be accomplished to one time, and a plurality of extraction tubes extend to the centrifuging tube respectively, once only can Europe accomplish the extraction to the interior supernatant of a plurality of centrifuging tubes, need not the staff and centrifuge and extract one by one, improve the efficiency that centrifugation and supernatant body extracted.
3. This preparation equipment of umbilical cord source mesenchymal stem cell exosome oral cavity restoration nebula's preparation has the rack that extends to the circular recess in the bottom inner wall sliding connection of rotation groove, and the rack is close to driving motor's one end and touches mutually with the special-shaped wheel, the bottom fixedly connected with of axis of rotation and the straight-teeth gear of rack looks meshing, along with the rotation of special-shaped wheel, the special-shaped wheel can make the rack reciprocating slide with the cooperation of extension spring, and then can drive the straight-teeth gear and wash the case and rotate to wash the case and drive the cleaning solution wherein and clean the filter screen, avoid the residual impurity on the filter screen, influence the purity that draws of later stage supernatant.
4. This nebula's preparation equipment is restoreed to umbilical cord source mesenchymal stem cell exosome oral cavity's slide is kept away from one side fixedly connected with connecting rod of first extension board, the hollow dish of other end fixedly connected with of connecting rod, the bottom of hollow dish is equipped with a plurality of extraction tubes that are linked together, the opposite side fixedly connected with of hollow dish and second extension board sliding connection's connecting plate, when the slide moves down, the slide passes through the connecting rod and drives hollow dish and move down, and then when the carousel was accomplished to the vertical centre gripping of centrifuging tube the extraction tube can extend to accomplish the extraction to the cleanness of extraction tube, hollow dish, feed liquor pipe and drain pipe or accomplish the extraction to the intraductal supernatant of centrifuging tube, and is simple and convenient, not only improves extraction efficiency and can also guarantee the purity of supernatant.
The centrifugal tube rack is simple in structure, the centrifugal tube and the placing disc can be clamped longitudinally and transversely by rotating the screw rod, the hollow disc can move downwards, supernatant can be extracted, and the extraction tube, the filter screen, the liquid inlet tube and the liquid outlet tube can be cleaned.
Drawings
Fig. 1 is a front sectional view of a preparation device of an umbilical cord-derived mesenchymal stem cell exosome oral repair spray provided by the invention;
fig. 2 is a three-dimensional sectional view of a workbench of a preparation device of the oral repair spray of umbilical cord-derived mesenchymal stem cell exosomes according to the present invention;
FIG. 3 is a three-dimensional view of a placing plate of the preparation device of the oral repair spray of umbilical cord-derived mesenchymal stem cell exosomes according to the present invention;
fig. 4 is a three-dimensional sectional view of a disc of a preparation device of the umbilical cord-derived mesenchymal stem cell exosome oral cavity repair spray provided by the invention;
FIG. 5 is a three-dimensional view of a rotating disc of the preparation device of the oral repair spray of umbilical cord-derived mesenchymal stem cell exosomes according to the present invention;
fig. 6 is a front view of an extraction assembly of the preparation device of the oral repair spray of umbilical cord-derived mesenchymal stem cell exosomes according to the present invention;
fig. 7 is a front sectional view of the preparation device of the oral repair spray of umbilical cord-derived mesenchymal stem cell exosomes according to the present invention in example two;
fig. 8 is an enlarged view of fig. 7 at a.
In the figure: 1. a work table; 2. a first support plate; 3. a second support plate; 4. placing a tray; 5. a placement groove; 6. centrifuging the tube; 7. a rotating groove; 8. a rotating shaft; 9. a drive motor; 10. a disc; 11. a moving groove; 12. a chute; 13. a bidirectional screw rod; 14. a slider; 15. a splint; 16. a first bevel gear; 17. a slide plate; 18. rotating the disc; 19. a screw; 20. a second bevel gear; 21. a sealing groove; 22. a connecting plate; 23. a hollow disc; 24. an extraction tube; 25. filtering with a screen; 26. a diaphragm pump; 27. a liquid inlet pipe; 28. a liquid outlet pipe; 29. a cleaning tank; 30. a circular groove; 31. a cylinder; 32. a rotating shaft; 33. a first turntable; 34. a spur gear; 35. a special-shaped wheel; 36. a rack; 37. a fixed block; 38. a tension spring; 39. a rotating plate; 40. a connecting rod; 41. a slide bar; 42. a limiting groove; 43. a limiting block; 44. a rectangular groove; 45. a wedge block; 46. a card slot; 47. a second turntable; 48. a spring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, are used in the orientations and positional relationships indicated in the drawings, which are based on the orientations and positional relationships indicated in the drawings, and are used for convenience of description and simplicity of description, but do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
Example 1:
referring to fig. 1-6, the preparation device of the umbilical cord source mesenchymal stem cell exosome oral restoration spraying agent comprises a workbench 1, wherein the top of the workbench 1 is fixedly connected with a first support plate 2 through a bolt, the top of the workbench 1 is provided with a placing plate 4, the top of the workbench 1 is provided with a circular groove 30, the bottom inner wall of the circular groove 30 is fixedly connected with a cylinder 31 through a bolt, the workbench 1 is internally provided with a clamping component for clamping the placing plate 4, the top end of the cylinder 31 is provided with an extraction component for extracting supernatant, the clamping component can control the lifting of the extraction component, the outer wall of the cylinder 31 is provided with a cleaning component for cleaning the extraction component, one side of the placing plate 4, which is far away from each other, is provided with a limit groove 42, the top of a second turntable 47 is fixedly connected with two limit blocks 43 matched with the limit grooves 42 through bolts, the limit grooves 42 and the limit blocks 43 are matched to limit the placing plate 4, prevent to place when dish 4 is placed on second carousel 47, later stage can't make in the accurate insert centrifuging tube 6 of extraction tube 24.
According to the invention, the clamping assembly comprises a rotating groove 7 arranged at the top of the workbench 1, a rotating shaft 8 is rotatably connected to the inner wall of the bottom of the rotating groove 7, a disc 10 is fixedly connected to the top end of the rotating shaft 8 through a bolt, a placing disc 4 is placed at the top of the disc 10, a moving groove 11 is arranged in the disc 10, two sliding grooves 12 communicated with the moving groove 11 are arranged at the top of the disc 10, a bidirectional screw 13 is transversely and rotatably connected in the moving groove 11, two sliding blocks 14 are sleeved on the outer wall threads of the bidirectional screw 13, the sliding blocks 14 are slidably connected with the inner wall of the bottom of the moving groove 11, the two sliding blocks 14 are respectively positioned on the positive and negative thread sections of the bidirectional screw 13, the top ends of the sliding blocks 14 penetrate through the sliding grooves 12 and are fixedly connected with clamping plates 15 through bolts, a first bevel gear 16 is fixedly sleeved on the outer wall of the bidirectional screw 13, a driving motor 9 is arranged in the workbench 1, and the output shaft of the driving motor 9 is fixedly connected with the bottom end of the rotating shaft 8 through a coupler.
According to the centrifugal tube placing device, one side, close to a placing disc 4, of a first support plate 2 is connected with a sliding plate 17 in a sliding mode, a rotating disc 18 is connected in a rotating mode in the sliding plate 17, a plurality of sealing grooves 21 are formed in the bottom of the rotating disc 18, a plurality of placing grooves 5 corresponding to the sealing grooves 21 are formed in the top of the placing disc 4, centrifugal tubes 6 are placed in the placing grooves 5, a screw 19 penetrates through an inner thread of the rotating disc 18, the bottom end of the screw 19 penetrates through the inner wall of the top of a moving groove 11 in a rotating mode, a second bevel gear 20 is fixedly connected to the inner wall of the top of the moving groove 11, the second bevel gear 20 is meshed with a first bevel gear 16, when the rotating disc 18 moves downwards to clamp the centrifugal tubes 6, the sliding blocks 14 and clamping plates 15 can move towards the middle, the placing disc 4 is clamped transversely, clamping in two directions is completed simultaneously, and cracking caused by shaking of the centrifugal tubes 6 during centrifugal motion is avoided.
In the invention, the extraction assembly comprises a second support plate 3 fixedly connected to the top end of a cylinder 31 through bolts, one side of the second support plate 3 close to a placing disc 4 is slidably connected with a connecting plate 22, the other side of the connecting plate 22 is fixedly connected with a hollow disc 23 through bolts, a plurality of extraction tubes 24 communicated with each other are arranged in the hollow disc 23, a filter screen 25 is fixedly sleeved on the outer wall of each extraction tube 24, a diaphragm pump 26 is fixedly connected to the top end of the second support plate 3 through bolts, a liquid inlet pipe 27 is fixedly sleeved on a liquid inlet of the diaphragm pump 26, the other end of the liquid inlet pipe 27 is communicated with the hollow disc 23, a liquid outlet of the diaphragm pump 26 is fixedly sleeved with a liquid outlet pipe 28, when a sliding plate 17 moves downwards, the sliding plate 17 drives the hollow disc 23 to move downwards through a connecting rod 40, and then when a rotating disc 18 finishes longitudinally clamping a centrifugal tube 6, the extraction tubes 24 can extend into a cleaning box 29 to finish longitudinal clamping of the extraction tubes 24, the hollow disc 23 and, The cleaning of feed liquor pipe 27 and drain pipe 28 or the completion are to the extraction of centrifuging tube 6 interior supernatant, and is simple and convenient, not only improves extraction efficiency but also can guarantee the purity of supernatant.
In the invention, the cleaning assembly comprises a rotating plate 39 rotatably sleeved on the outer wall of the cylinder 31, a rotating shaft 32 is rotatably penetrated through the rotating plate 39, the top end of the rotating shaft 32 is fixedly connected with a first rotating disc 33 through a bolt, the top of the first rotating disc 33 is provided with a cleaning box 29, the bottom end of the rotating shaft 32 is fixedly connected with a straight gear 34, and the top of one side of the rotating plate 39, which is far away from the first rotating disc 33, is fixedly connected with a second rotating disc 47.
According to the invention, the inner wall of the bottom of the circular groove 30 is connected with the rack 36 meshed with the straight gear 34 in a sliding manner, the top of the rack 36 is fixedly connected with the fixed block 37 through a bolt, one side of the fixed block 37, which is far away from the second support plate 3, is fixedly connected with the tension spring 38, the other end of the tension spring 38 is fixedly connected with the inner wall of one side of the circular groove 30, the outer wall of the rotating shaft 8 is fixedly sleeved with the special-shaped wheel 35, one end of the rack 36, which is far away from the second support plate 3, is extended into the rotating groove 7 in a sliding manner and is contacted with the special-shaped wheel 35, the rack 36 can slide back and forth through the matching of the special-shaped wheel 35 and the tension spring 38 along with the rotation of the special-shaped wheel 35, and further can drive the straight gear 34 and the cleaning box 29 to rotate, so that the cleaning liquid in the cleaning box 29 drives the cleaning liquid to clean the filter screen 25, and the impurity remained on the filter screen 25 is avoided, and the extraction purity of the supernatant in the later period is influenced.
According to the invention, a plurality of centrifuge tubes 6 can be arranged on the placing disc 4, so that centrifugation of the centrifuge tubes 6 can be completed at one time, the extraction tubes 24 respectively extend into the centrifuge tubes 6, extraction of supernatant in the centrifuge tubes 6 can be completed at one time, workers do not need to perform centrifugation and extraction one by one, and the centrifugation and supernatant extraction efficiency is improved.
In the invention, the top of the disc 10 is fixedly connected with a sliding rod 41 through a bolt, the top end of the sliding rod 41 penetrates through the rotating disc 18 in a sliding manner, and the sliding rod 41 can play a role in guiding the rotating disc 18, so that the rotating disc 18 is prevented from inclining.
According to the invention, one side of the sliding plate 17, which is far away from the first support plate 2, is fixedly connected with the connecting rod 40 through the bolt, the other end of the connecting rod 40 is fixedly connected with the hollow disc 23 through the bolt, and the clamping assembly and the extracting assembly can be connected together through the connecting rod 40, so that the lifting of the extracting assembly can be controlled by the clamping assembly conveniently in the later period.
Example 2:
referring to fig. 1-8, the preparation device of the umbilical cord source mesenchymal stem cell exosome oral restoration spraying agent comprises a workbench 1, wherein the top of the workbench 1 is fixedly connected with a first support plate 2 through a bolt, the top of the workbench 1 is provided with a placing plate 4, the top of the workbench 1 is provided with a circular groove 30, the bottom inner wall of the circular groove 30 is fixedly connected with a cylinder 31 through a bolt, the workbench 1 is internally provided with a clamping component for clamping the placing plate 4, the top end of the cylinder 31 is provided with an extraction component for extracting supernatant, the clamping component can control the lifting of the extraction component, the outer wall of the cylinder 31 is provided with a cleaning component for cleaning the extraction component, one side of the placing plate 4, which is far away from each other, is provided with a limit groove 42, the top of a second turntable 47 is fixedly connected with two limit blocks 43 matched with the limit grooves 42 through bolts, the limit grooves 42 and the limit blocks 43 are matched to limit the placing plate 4, prevent to place when dish 4 is placed on second carousel 47, later stage can't make in the accurate insert centrifuging tube 6 of extraction tube 24.
According to the invention, the clamping assembly comprises a rotating groove 7 arranged at the top of the workbench 1, a rotating shaft 8 is rotatably connected to the inner wall of the bottom of the rotating groove 7, a disc 10 is fixedly connected to the top end of the rotating shaft 8 through a bolt, a placing disc 4 is placed at the top of the disc 10, a moving groove 11 is arranged in the disc 10, two sliding grooves 12 communicated with the moving groove 11 are arranged at the top of the disc 10, a bidirectional screw 13 is transversely and rotatably connected in the moving groove 11, two sliding blocks 14 are sleeved on the outer wall threads of the bidirectional screw 13, the sliding blocks 14 are slidably connected with the inner wall of the bottom of the moving groove 11, the two sliding blocks 14 are respectively positioned on the positive and negative thread sections of the bidirectional screw 13, the top ends of the sliding blocks 14 penetrate through the sliding grooves 12 and are fixedly connected with clamping plates 15 through bolts, a first bevel gear 16 is fixedly sleeved on the outer wall of the bidirectional screw 13, a driving motor 9 is arranged in the workbench 1, and the output shaft of the driving motor 9 is fixedly connected with the bottom end of the rotating shaft 8 through a coupler.
According to the centrifugal tube placing device, one side, close to a placing disc 4, of a first support plate 2 is connected with a sliding plate 17 in a sliding mode, a rotating disc 18 is connected in a rotating mode in the sliding plate 17, a plurality of sealing grooves 21 are formed in the bottom of the rotating disc 18, a plurality of placing grooves 5 corresponding to the sealing grooves 21 are formed in the top of the placing disc 4, centrifugal tubes 6 are placed in the placing grooves 5, a screw 19 penetrates through an inner thread of the rotating disc 18, the bottom end of the screw 19 penetrates through the inner wall of the top of a moving groove 11 in a rotating mode, a second bevel gear 20 is fixedly connected to the inner wall of the top of the moving groove 11, the second bevel gear 20 is meshed with a first bevel gear 16, when the rotating disc 18 moves downwards to clamp the centrifugal tubes 6, the sliding blocks 14 and clamping plates 15 can move towards the middle, the placing disc 4 is clamped transversely, clamping in two directions is completed simultaneously, and cracking caused by shaking of the centrifugal tubes 6 during centrifugal motion is avoided.
In the invention, the extraction assembly comprises a second support plate 3 fixedly connected to the top end of a cylinder 31 through bolts, one side of the second support plate 3 close to a placing disc 4 is slidably connected with a connecting plate 22, the other side of the connecting plate 22 is fixedly connected with a hollow disc 23 through bolts, a plurality of extraction tubes 24 communicated with each other are arranged in the hollow disc 23, a filter screen 25 is fixedly sleeved on the outer wall of each extraction tube 24, a diaphragm pump 26 is fixedly connected to the top end of the second support plate 3 through bolts, a liquid inlet pipe 27 is fixedly sleeved on a liquid inlet of the diaphragm pump 26, the other end of the liquid inlet pipe 27 is communicated with the hollow disc 23, a liquid outlet of the diaphragm pump 26 is fixedly sleeved with a liquid outlet pipe 28, when a sliding plate 17 moves downwards, the sliding plate 17 drives the hollow disc 23 to move downwards through a connecting rod 40, and then when a rotating disc 18 finishes longitudinally clamping a centrifugal tube 6, the extraction tubes 24 can extend into a cleaning box 29 to finish longitudinal clamping of the extraction tubes 24, the hollow disc 23 and, The cleaning of feed liquor pipe 27 and drain pipe 28 or the completion are to the extraction of centrifuging tube 6 interior supernatant, and is simple and convenient, not only improves extraction efficiency but also can guarantee the purity of supernatant.
In the invention, the cleaning assembly comprises a rotating plate 39 rotatably sleeved on the outer wall of the cylinder 31, a rotating shaft 32 is rotatably penetrated through the rotating plate 39, the top end of the rotating shaft 32 is fixedly connected with a first rotating disc 33 through a bolt, the top of the first rotating disc 33 is provided with a cleaning box 29, the bottom end of the rotating shaft 32 is fixedly connected with a straight gear 34, and the top of one side of the rotating plate 39, which is far away from the first rotating disc 33, is fixedly connected with a second rotating disc 47.
According to the invention, the inner wall of the bottom of the circular groove 30 is connected with the rack 36 meshed with the straight gear 34 in a sliding manner, the top of the rack 36 is fixedly connected with the fixed block 37 through a bolt, one side of the fixed block 37, which is far away from the second support plate 3, is fixedly connected with the tension spring 38, the other end of the tension spring 38 is fixedly connected with the inner wall of one side of the circular groove 30, the outer wall of the rotating shaft 8 is fixedly sleeved with the special-shaped wheel 35, one end of the rack 36, which is far away from the second support plate 3, is extended into the rotating groove 7 in a sliding manner and is contacted with the special-shaped wheel 35, the rack 36 can slide back and forth through the matching of the special-shaped wheel 35 and the tension spring 38 along with the rotation of the special-shaped wheel 35, and further can drive the straight gear 34 and the cleaning box 29 to rotate, so that the cleaning liquid in the cleaning box 29 drives the cleaning liquid to clean the filter screen 25, and the impurity remained on the filter screen 25 is avoided, and the extraction purity of the supernatant in the later period is influenced.
According to the invention, a plurality of centrifuge tubes 6 can be arranged on the placing disc 4, so that centrifugation of the centrifuge tubes 6 can be completed at one time, the extraction tubes 24 respectively extend into the centrifuge tubes 6, extraction of supernatant in the centrifuge tubes 6 can be completed at one time, workers do not need to perform centrifugation and extraction one by one, and the centrifugation and supernatant extraction efficiency is improved.
In the invention, the top of the disc 10 is fixedly connected with a sliding rod 41 through a bolt, the top end of the sliding rod 41 penetrates through the rotating disc 18 in a sliding manner, and the sliding rod 41 can play a role in guiding the rotating disc 18, so that the rotating disc 18 is prevented from inclining.
In the invention, one side of the sliding plate 17, which is far away from the first support plate 2, is fixedly connected with the connecting rod 40 through the bolt, the other end of the connecting rod 40 is fixedly connected with the hollow disc 23 through the bolt, and the clamping assembly and the extraction assembly can be connected together through the connecting rod 40, so that the later-stage clamping assembly can conveniently control the extraction assembly to lift.
In the invention, the inner walls of the sides of the circular grooves 30 far away from each other are provided with rectangular grooves 44, wedge blocks 45 are connected in the rectangular grooves 44 in a sliding way, one ends of the wedge blocks 45 far away from the second support plate 3 are fixedly connected with springs 48, the other end of the spring 48 is fixedly connected with the inner wall of one side of the rectangular groove 44, a clamping groove 46 clamped with the wedge block 45 is arranged on one side of the second rotary disc 47 far away from the second support plate 3, the rotary plate 39 is rotated to enable the rotary plate 39 to rotate 180 degrees, the rotary plate 39 drives the second rotary disc 47 and the placing disc 4 to rotate, when the wedge-shaped block 45 rotates 180 degrees, the wedge-shaped block 45 is pushed to be clamped into the clamping groove 46 under the elastic force action of the spring 48, the second rotating disc 47 is limited, then the screw 19 is rotated to make the rotating disc 18 drive the connecting rod 40 and the hollow disc 23 to move downwards, the extraction tube 24 and the filter screen 25 respectively extend into the centrifuge tube 6, and the centrifuge tube 6 on the second rotating disc 47 can be aligned with the extraction tube 24.
The working principle is as follows: respectively placing a plurality of centrifuge tubes 6 filled with cells in a placing groove 5, placing a placing disc 4 on a disc 10, aligning a limiting groove 42 with a clamping plate 15, rotating a screw rod 19, connecting the screw rod 19 with a rotating disc 18 through threads, moving the rotating disc 18 downwards, extending the top end of the centrifuge tube 6 into a sealing groove 21 along with the downward movement of the rotating disc 18, sealing the centrifuge tube 6, fixing the centrifuge tube 6 longitudinally through the cooperation of the rotating disc 18 and the placing disc 4, driving a second bevel gear 20 to rotate while the screw rod 19 rotates, meshing the second bevel gear 20 with a first bevel gear 16, driving a bidirectional screw rod 13 to rotate through the first bevel gear 16, connecting the bidirectional screw rod 13 with a sliding block 14 through threads, driving the clamping plate 15 to rotate towards the middle through the sliding block 14 along with the rotation of the bidirectional screw rod 13, extending the clamping plate 15 into the limiting groove 42, further fixedly clamping the placing disc 4, and fixing the centrifuge tube 6 while the rotating disc 18 moves downwards, the rotating disc 18 drives the hollow disc 23 and the extraction tube 24 to move downwards through the connecting rod 40, the extraction tube 24 extends into the cleaning box 29, the diaphragm pump 26 is started, the cleaning liquid in the cleaning box 29 is pumped out from the liquid outlet tube 28 through the liquid inlet tube 27 and the extraction tube 24 by the diaphragm pump 26, the hollow disc 23, the extraction tube 24, the liquid inlet tube 27 and the liquid outlet tube 28 can be cleaned, the influence of impurities remained in the hollow disc 23, the extraction tube 24, the liquid inlet tube 27 and the liquid outlet tube 28 on the purity of supernatant liquid extraction in the later period is avoided, the driving motor 9 is started to drive the rotating shaft 8 to rotate, the rotating shaft 8 drives the special-shaped wheel 35 and the disc 10 to rotate, the disc 10 drives the placing disc 4 and the plurality of centrifuge tubes 6 to rotate for centrifugal motion, further, the centrifugal separation is carried out on the cells in the centrifuge tubes 6, when the disc 10 and the special-shaped wheel 35 rotate, the protruded part of the special-shaped wheel 35 pushes the rack 36 to move towards the second support plate 3, the tension spring 38 starts to stretch, the rack 36 is meshed with the straight gear 34, the rack 36 drives the straight gear 34, the rotating shaft 32 and the cleaning box 29 to rotate, when the rotation of the cleaning box 29 can enable the extracting tube 24 to pump cleaning liquid in the cleaning box 29, the cleaning liquid in the cleaning box 29 can rotate along with the cleaning box 29, residual impurities on the surface layer of the filter screen 25 are removed, after the centrifugal tube 6 is separated, the centrifugal tube 6 is settled for 5 minutes, the screw rod 19 is reversely rotated, clamping and fixing of the rotating disc 18 and the clamping plate 15 on the placing disc 4 are removed, the placing disc 4 is taken out of the disc 10 and placed on the second rotating disc 47, the limiting block 43 just extends into the limiting groove 42 to limit the placing disc 4, the rotating plate 39 is rotated to enable the rotating plate 39 to rotate 180 degrees, the rotating plate 39 drives the second rotating disc 47 and the placing disc 4 to rotate, when the rotating plate rotates 180 degrees, the wedge block 45 pushes the wedge block 45 to be clamped into the clamping groove 46 under the elastic force of the spring 48, carry on spacingly to second carousel 47, rotate screw rod 19 afterwards and make rolling disc 18 drive connecting rod 40 and hollow dish 23 move down, during extraction tube 24 and filter screen 25 extend to centrifuging tube 6 respectively, start diaphragm pump 26, extract through the supernatant of extraction tube 24 and filter screen 25 in with centrifuging tube 6, can filter the supernatant in centrifuging tube 6 through filter screen 25, avoid diaphragm pump 26 to extract broken cell.
However, the working principle and wiring method of the driving motor 9 and the diaphragm pump 26, which are well known to those skilled in the art, are conventional means or common knowledge in the art, and thus will not be described in detail herein, and those skilled in the art can make any choice according to their needs or convenience.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.
Claims (10)
1. The preparation equipment of the umbilical cord source mesenchymal stem cell exosome oral repair spraying agent comprises a workbench (1), and is characterized in that the top of the workbench (1) is fixedly connected with a first support plate (2), the top of the workbench (1) is provided with a placing plate (4), the top of the workbench (1) is provided with a circular groove (30), and the inner wall of the bottom of the circular groove (30) is fixedly connected with a cylinder (31);
a clamping assembly for clamping the placing disc (4) is arranged in the workbench (1);
an extraction component for extracting supernatant is arranged at the top end of the cylinder (31), and the clamping component can control the extraction component to lift;
the outer wall of the cylinder (31) is provided with a cleaning component for cleaning the extraction component.
2. The preparation equipment of umbilical cord source mesenchymal stem cell exosome oral cavity restoration spraying agent according to claim 1, wherein the clamping assembly comprises a rotating groove (7) arranged at the top of the workbench (1), the inner wall of the bottom of the rotating groove (7) is rotatably connected with a rotating shaft (8), the top end of the rotating shaft (8) is fixedly connected with a disc (10), a placing disc (4) is placed at the top of the disc (10), a moving groove (11) is arranged in the disc (10), the top of the disc (10) is provided with two sliding grooves (12) communicated with the moving groove (11), and a bidirectional screw rod (13) is transversely rotatably connected in the moving groove (11).
3. The preparation equipment of umbilical cord source mesenchymal stem cell exosome oral restoration spraying agent according to claim 1, wherein one side of the first support plate (2) close to the placing disc (4) is slidably connected with a sliding plate (17), the sliding plate (17) is rotatably connected with a rotating disc (18), the bottom of the rotating disc (18) is provided with a plurality of sealing grooves (21), the top of the placing disc (4) is provided with a plurality of placing grooves (5) corresponding to the sealing grooves (21), a centrifuge tube (6) is placed in the placing groove (5), a screw rod (19) penetrates through the inner thread of the rotating disc (18), the bottom end of the screw rod (19) rotates to penetrate through the inner wall of the top of the moving groove (11) and is fixedly connected with a second bevel gear (20), and the second bevel gear (20) is meshed with the first bevel gear (16).
4. The preparation device of the oral repair spray of umbilical cord-derived mesenchymal stem cell exosomes according to claim 1, it is characterized in that the extraction component comprises a second support plate (3) fixedly connected with the top end of the cylinder (31), one side of the second support plate (3) close to the placing disc (4) is connected with a connecting plate (22) in a sliding way, the other side of the connecting plate (22) is fixedly connected with a hollow disc (23), a plurality of communicated extraction pipes (24) are arranged in the hollow disc (23), a filter screen (25) is fixedly sleeved on the outer wall of the extraction pipe (24), a diaphragm pump (26) is fixedly connected with the top end of the second support plate (3), a liquid inlet pipe (27) is fixedly sleeved on a liquid inlet of the diaphragm pump (26), and the other end of the liquid inlet pipe (27) is communicated with the hollow disc (23), and a liquid outlet of the diaphragm pump (26) is fixedly sleeved with a liquid outlet pipe (28).
5. The preparation equipment of umbilical cord source mesenchymal stem cell exosome oral cavity restoration nebula of claim 1, wherein, the clean subassembly includes that the rotation cover establishes rotor plate (39) at cylinder (31) outer wall, rotation axis (32) has been run through in rotor plate (39), the first carousel of top fixedly connected with (33) of rotation axis (32), the top of first carousel (33) is equipped with washs case (29), the bottom fixedly connected with spur gear (34) of rotation axis (32), one side top fixedly connected with second carousel (47) of first carousel (33) is kept away from to rotor plate (39).
6. The preparation equipment of umbilical cord source mesenchymal stem cell exosome oral cavity restoration spraying agent according to claim 1, wherein the bottom inner wall of circular groove (30) is connected with rack (36) meshed with spur gear (34) in a sliding manner, the top fixedly connected with fixed block (37) of rack (36), one side fixedly connected with extension spring (38) of second extension board (3) is kept away from to fixed block (37), and the other end of extension spring (38) is fixedly connected with one side inner wall of circular groove (30), the outer wall fixed cover of pivot (8) is equipped with special-shaped wheel (35), the one end that second extension board (3) was kept away from to rack (36) slides and extends to in rotating groove (7) and touches with special-shaped wheel (35).
7. The preparation device of oral repair spray for umbilical cord derived mesenchymal stem cell exosomes according to any one of claims 1 to 5, wherein the placing discs (4) are provided with a limiting groove (42) on the sides far away from each other, and two limiting blocks (43) matched with the limiting groove (42) are fixedly connected to the top of the second rotating disc (47).
8. The preparation device of the oral repair spray of umbilical cord-derived mesenchymal stem cell exosomes according to claim 2, it is characterized in that the clamping component also comprises two sliding blocks (14) which are sleeved on the outer wall of the bidirectional screw rod (13) through threads, the sliding blocks (14) are connected with the inner wall of the bottom of the moving groove (11) in a sliding way, the two sliding blocks (14) are respectively positioned on the positive and negative thread sections of the bidirectional screw rod (13), the top end of the sliding block (14) penetrates through the sliding groove (12) and is fixedly connected with a clamping plate (15), a first bevel gear (16) is fixedly sleeved on the outer wall of the bidirectional screw rod (13), a driving motor (9) is arranged in the workbench (1), an output shaft of the driving motor (9) is fixedly connected with the bottom end of the rotating shaft (8), the top of the disc (10) is fixedly connected with a sliding rod (41), and the top end of the sliding rod (41) penetrates through the rotating disc (18) in a sliding mode.
9. The preparation device of the oral repair spray of umbilical cord derived mesenchymal stem cell exosomes according to claim 3, wherein one side of the sliding plate (17) far away from the first support plate (2) is fixedly connected with a connecting rod (40), and the other end of the connecting rod (40) is fixedly connected with the hollow disc (23).
10. The preparation device of the oral repair spray for umbilical cord derived mesenchymal stem cell exosomes according to claim 1, wherein the inner walls of the sides of the circular grooves (30) far away from each other are provided with rectangular grooves (44), wedge blocks (45) are slidably connected in the rectangular grooves (44), one ends of the wedge blocks (45) far away from the second support plate (3) are fixedly connected with springs (48), the other ends of the springs (48) are fixedly connected with the inner wall of one side of the rectangular grooves (44), and one side of the second turntable (47) far away from the second support plate (3) is provided with a clamping groove (46) clamped with the wedge blocks (45).
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