CN114669082A

CN114669082A - Radial and tangential flow bottoms for chromatographic column and chromatographic system combined with radial and tangential flow bottoms

Info

Publication number: CN114669082A
Application number: CN202210313676.0A
Authority: CN
Inventors: 赵庆利; 金祖伟; 周胜; 黄宇峰; 金博闻
Original assignee: Lisure Science Suzhou Co ltd
Current assignee: Lisure Science Suzhou Co ltd
Priority date: 2022-03-28
Filing date: 2022-03-28
Publication date: 2022-06-28

Abstract

The invention discloses a radial and tangential flow bottom for a chromatographic column and a chromatographic system combined with the radial and tangential flow bottom. The flow bottom comprises a sieve plate, a fixing ring, a bus bar and a flange plate, wherein the sieve plate is fixed on the upper side of the sieve plate fixing ring, the lower side of the sieve plate fixing ring is arranged on the bus bar, and the flange plate is arranged below the bus bar; wherein, form a horizontal runner of going up between cylinder manifold and the sieve, form a horizontal runner down between cylinder manifold and the ring flange, wherein, be equipped with the vertical runner hole of round on the cylinder manifold, each runner hole all communicates runner and runner down. The radial and tangential flow bottom of the chromatographic column and the chromatographic system combined with the radial and tangential flow bottom can simultaneously realize tangential flow filtration and chromatographic loading, realize high-efficiency uniform distribution effect of fluid, have excellent distribution effect, can circularly load the sample and use a balanced liquid cleaning mode, thereby realizing high linear flow rate and overload loading, realizing extremely high dynamic loading capacity, and in addition, the sieve plate can be backwashed and is not easy to block.

Description

Radial and tangential flow bottom for chromatographic column and chromatographic system combined with radial and tangential flow bottom

Technical Field

The invention relates to the field of chromatographic columns, in particular to a radial and tangential flow bottom for a chromatographic column and a chromatographic system combined with the radial and tangential flow bottom.

Background

The large-scale chromatographic separation technology is widely applied to the separation and purification of biopharmaceuticals and general macromolecular materials, and is a key technology for industrialized high-purity separation. Chromatographic separation steps typically require pretreatment of the material, whereas existing column head dispensers for chromatographic columns do not have the ability to handle particulate or precipitated material and therefore must be used in conjunction with centrifugation and filtration equipment. Therefore, although the separation efficiency is high, the number of steps is generally large and the number of steps is long.

In addition, due to the large flow resistance of the column, the flux through the column is usually low, often becoming a bottleneck in the overall downstream process.

The existing chromatographic column cap technology can not process particle precipitation materials, and can not realize back flush cleaning of a distribution sieve plate, so that the existing technology can not realize the capacity of simultaneously carrying out high-efficiency chromatography and particle processing.

In the prior art, the lower extreme of the chromatographic column of chromatographic column cap includes sieve, lower cover and backup pad down, and the central point of lower sieve puts intercommunication liquid outlet, and for example the patent number is: 202120067489.X, entitled a hydraulic drive type dynamic chromatography device, when needing to be filled with separation liquid, the liquid is injected from a liquid inlet and flows out from a liquid outlet for collection; or, for example, the following patent numbers: 201820028656.8 the invention relates to a moving beam type full-automatic chromatography column, the bottom of which adopts a structure of sieve plate, splitter plate and base, the center position of the sieve plate is provided with a lower spray head, when the separated liquid needs to be filled, the liquid enters from the chromatography port of the upper spray head and flows out from the chromatography port of the lower spray head for collection, or enters from the chromatography port of the lower spray head and flows out from the chromatography port of the upper spray head for collection.

Structurally, the separation liquid enters from one end of the chromatographic column and flows out from the other end for collection, and the disadvantages are that: 1. washing or elution of the upper part of the column head cannot be realized; 2. does not support the back flushing of the filter plate (sieve plate), and is easy to cause blockage; 3. high linear flow rate and overload sample loading cannot be realized simultaneously, and the dynamic loading capacity is low.

Disclosure of Invention

To solve the above problems, the present invention provides a chromatography system for radial and tangential flow bottoms of chromatography columns and combinations thereof.

According to one aspect of the invention, a radial and tangential flow bottom for a chromatography column is provided, which comprises a sieve plate, a fixing ring, a confluence plate and a flange plate, wherein the sieve plate is fixed on the upper side of the sieve plate fixing ring, the lower side of the sieve plate fixing ring is installed on the confluence plate, and the flange plate is installed below the confluence plate; the flow collecting plate is provided with a circle of vertical flow channel holes, and each flow channel hole is communicated with the upper flow channel and the lower flow channel.

In some embodiments, the middle of the bus bar protrudes upwards, and the screen plate fixing ring is sleeved on the protruding part of the bus bar. Thus, the manner in which the screen plate retaining ring is mounted on the manifold plate is described.

In some embodiments, the upper surface of the manifold plate is sloped toward and forms a slope with the upper surface of the flange plate being sloped toward and forms a slope with the center thereof. Thereby, the upper flow path 5 and/or the lower flow path 6 can be inclined toward the center.

In some embodiments, the lower surface of the bus plate is also inclined toward the center thereof and forms a slope. Thereby, the upper flow path 5 and/or the lower flow path 6 can be further inclined toward the center.

In some embodiments, a plurality of circles of vertical balancing holes are arranged on the bus plate, and the diameter of each balancing hole on the outer circle is larger than that of each balancing hole on the inner circle. From this, can be used for the pressure of balanced cylinder manifold upper and lower both sides through setting up the balancing hole.

In some embodiments, the diameter of the upper and lower openings of the balancing hole is larger than that of the central portion, and the longitudinal section of the balancing hole has a trapezoidal structure with symmetrical upper and lower parts and a rectangular structure in the middle. Thus, a specific structure of the balance hole is described.

According to one aspect of the invention, a chromatography system used with the radial and tangential flow bottom for a chromatography column comprises a spray head, a liquid inlet pipe and a return pipe, wherein the spray head sequentially penetrates through the centers of the flange plate, the confluence plate and the sieve plate from bottom to top, the spray head is provided with a top cover, a side wall, a base, a valve rod, a baffle plate and a cylinder, a cavity is formed among the top cover, the side wall and the base, the top of the side wall is provided with a through hole, and the base is provided with a vertical groove;

the baffle is arranged between the side wall and the base, one side of the baffle is fixed on the bus board, the other side of the baffle extends into the cavity, the top of the valve rod protrudes towards two sides and is positioned above the part of the baffle extending to the cavity, and the cylinder is connected with the bottom of the valve rod.

In some embodiments, the top cover is mounted below the center of the screen panel, the side walls are mounted at the center of the bus bar, and the base is mounted at the middle of the flange. Thus, the mounting positions of the top cover, side walls and base are described.

In some embodiments, the liquid inlet pipe passes through the base and is communicated with the cavity, the upper flow passage is communicated with the cavity through the through hole, and the lower flow passage is communicated with the return pipe through the groove. Thus, the way in which the interior of the spray head communicates with the liquid inlet pipe and the return pipe etc. is described.

In some embodiments, the cylinder is fixedly mounted on the base and its piston rod is connected to the bottom of the valve stem. Thus, specific mounting locations and manners of the cylinders are described.

The invention has the beneficial effects that:

1. according to the invention, the upper flow channel is formed between the confluence plate and the sieve plate, the lower flow channel is formed between the confluence plate and the flange plate, a radial and tangential flow structure is generated, and the sieve plate is matched to realize tangential flow filtration and chromatography sample loading at the same time, wherein the efficient fluid uniform distribution effect is realized by radial tangential flow, the distribution effect is excellent, the column effect is extremely high, and the sieve plate can be backwashed and is not easy to block;

2. by adopting a radial and tangential flow structure, the sample can be loaded through the tangential flow at the bottom of the column, and simultaneously the upper part of the column head can be washed and eluted simultaneously, and the sample is loaded circularly and is cleaned by using the balanced liquid, so that the high linear flow rate and overload sample loading are realized, and the extremely high dynamic loading capacity is realized.

Drawings

FIG. 1 is a cross-sectional view of a chromatography system for a radial, tangential flow base of a chromatography column and combinations thereof, according to one embodiment of the invention;

FIG. 2 is a top view of the bus bar shown in FIG. 1;

FIG. 3 is a cross-sectional view of the radial, tangential flow base of the chromatography column of FIG. 1 and a spray head of a chromatography system with which it is used when closed;

FIG. 4 is a cross-sectional view of the radial, tangential flow base of the chromatography column of FIG. 1 shown in FIG. 1 with the spray head of the chromatography system with which it is used open.

In the figure: the device comprises a sieve plate 1, a sieve plate fixing ring 2, a confluence plate 3, a flange plate 4, an upper flow channel 5, a lower flow channel 6, a spray head 7, a liquid inlet pipe 8, a return pipe 9, a flow channel hole 10, a balance hole 11, a top cover 71, a side wall 72, a base 73, a cavity 74, a through hole 75, a groove 76, a valve rod 77, a baffle 78 and a cylinder 79.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

FIG. 1 schematically shows a cross-sectional view of a chromatography system for a radial, tangential flow base of a chromatography column and its associated use, according to one embodiment of the invention, and FIG. 2 shows a top view of the manifold plate of FIG. 1. As shown in fig. 1-2, the system mainly comprises a sieve plate 1, a fixing ring 2, a confluence plate 3, a flange plate 4, a spray head 7, a liquid inlet pipe 8, a return pipe 9 and the like. The sieve 1 is fixed on one of them one side of the solid fixed ring of sieve 2 (establish to the upside), and the solid fixed ring of sieve 2's opposite side (establish to the downside) is installed on cylinder manifold 3, and wherein, the middle part of cylinder manifold 3 is upwards salient, and the solid fixed ring of sieve 2 overlaps to establish and installs on the bulge of cylinder manifold 3.

In addition, the flange plate 4 is installed below the collecting plate 3, and the spray head 7 sequentially penetrates through the flange plate 4, the collecting plate 3 and the center of the sieve plate 1 from bottom to top.

A transverse upper runner 5 is formed between the confluence plate 3 and the sieve plate 1, a transverse lower runner 6 is formed between the confluence plate 3 and the flange plate 4, a circle of vertical runner holes 10 are arranged at the edge of the confluence plate 3, which is slightly close to the sieve plate 1, and each runner hole 10 is communicated with the upper runner 5 and the lower runner 6. After the liquid flows into the upper flow path 5 through one side of the head 7, the liquid can flow back to the other side of the head 7 through the flow path holes 10 and the lower flow path 6.

The upper flow channel 5 and/or the lower flow channel 6 may be arranged inclined towards the centre in order to facilitate drainage in counter-flow. Wherein the following structure may be employed: inclining the upper surface of the bus plate 3 toward the center thereof and forming an inclined surface; while inclining and beveling the upper surface of the flange 4 toward its own center so that the upper flow path 5 and/or the lower flow path 6 are inclined toward the center. Further, it is also possible to incline and form the lower surface of the bus plate 3 to the center thereof.

Be provided with the vertical balanced hole 11 of many circles on the cylinder manifold 3 for the pressure of balanced cylinder manifold 3 upper and lower both sides, when pressure is equal, balanced liquid can be by the sieve enter into the main part of chromatographic column in, realize radial radiation's tangential flow, reach and realize simultaneously that tangential flow filters and chromatogram are gone up the appearance.

The circles of balancing holes 11 are relatively uniformly distributed between the center of the bus bar 3 and one circle of flow channel holes 10, and the number of circles of balancing holes 11 is set according to the real-time area of the bus bar, which is only schematically shown in fig. 2.

In addition, the remaining holes in fig. 2 are all fixing bolt holes for fixing the bottom of the chromatography column (not shown in the figure), the edges between the bus bar 3 and the flange plate 4, the fixing between the bus bar 3 and the flange plate 4, and the fixing between the bus bar 3 and the sieve plate 1.

The diameter of the upper opening and the lower opening of the balance hole 11 is larger than that of the central part, and the longitudinal section of the balance hole is in a trapezoidal structure with the upper part and the lower part being symmetrical and in a rectangular structure with the middle part. The aperture of the balancing hole 11 positioned on the outer ring is larger than that of the balancing hole 11 positioned on the inner ring, and the balancing hole 11 positioned on the innermost ring is positioned at the lowest point of the center of the confluence plate, so that the size and the orientation of the aperture of the balancing hole 11 can enable the resistance of the balancing hole 11 on the outer ring to the fluid flow to be smaller than the resistance of the balancing hole 11 on the inner ring to the fluid flow.

The spray head 7 has a top cover 71, a side wall 72, a base 73, a valve rod 77, a baffle plate 78 and a cylinder 79, wherein the top cover 71 is fixedly arranged below the central position of the screen plate 1, the side wall 72 is fixedly arranged at the central position of the confluence plate 3, the base 73 is fixedly arranged at the middle position of the flange plate 4, a cavity 74 is formed among the top cover 71, the side wall 72 and the base 73, and the liquid inlet pipe 8 penetrates through the base 73 and is communicated with the cavity 74.

The top of the side wall 72 is provided with a through hole 75, the through hole 75 is capable of communicating the upper flow path 5 with the cavity 74, while a vertical groove 76 is provided on the base 73, and the groove 76 is capable of communicating the lower flow path 6 with the return pipe 9.

The baffle plate 78 and the cylinder 79 together form a valve which is arranged in the cavity 74, wherein the baffle plate 78 is arranged between the side wall 72 and the base 73, one side of the baffle plate 78 is fixed to the collector plate 3, and the other side of the baffle plate 78 extends into the cavity 74 and has a smaller length than the cavity 74.

The valve rod 77 is formed in a substantially T-shaped longitudinal section, and the top thereof protrudes to both sides and is located above the portion of the baffle plate 78 extending to the cavity 74, thereby controlling the opening and closing of the upper and lower portions of the cavity 74.

Further, a cylinder 79 is fixedly installed on the base 73, and a piston rod thereof is connected to the bottom of the valve rod 77, and the cylinder 79 can pull the valve rod 77 to move up and down.

FIG. 3 shows a cross-sectional view of the radial and tangential flow base of the chromatography column of FIG. 1 and the showerhead of the chromatography system with which it is used closed. As shown in fig. 3, when the nozzle 7 is in a closed state, the piston rod of the cylinder 79 moves downward to drive the valve rod 77 to move downward, and when the top of the valve rod 77 contacts the baffle plate 78, the baffle plate 78 forms a closed circular baffle plate with the top of the valve rod 77 to separate the upper half part and the lower half part of the cavity 74, and the liquid inlet pipe 8 is disconnected from the upper flow passage 5. At this time, the column head of the column may be washed or eluted with an equilibration solution or an elution solution.

FIG. 4 shows a cross-sectional view of the radial and tangential flow base of the chromatography column of FIG. 1 and the showerhead of the chromatography system with which it is used when it is open. As shown in fig. 4, when the nozzle head 7 is in an open state, the piston rod of the air cylinder 79 moves upward to drive the valve rod 77 to move upward, the top of the valve rod 77 is separated from the baffle plate 78, and the upper half and the lower half of the cavity 74 are communicated.

When the bottom sieve plate needs to be backwashed, the balance liquid is pumped into the cavity 74 through the liquid inlet pipe 9, then enters the upper flow channel 5 through the through hole 75, then enters the lower flow channel 6 through the flow channel hole 10 and the balance hole 11, finally flows back into the return pipe 9 through the groove 76, and meanwhile, when the pressure is equal, the balance liquid can enter the main body of the chromatographic column through the sieve plate, so that the backwashing is realized. Therefore, the sample is circularly loaded and is cleaned by the balance liquid, so that high linear flow rate and overload sample loading can be realized, and extremely high dynamic loading capacity is realized.

What has been described above are merely some embodiments of the present invention. It will be apparent to those skilled in the art that various changes and modifications can be made without departing from the inventive concept thereof, and these changes and modifications can be made without departing from the spirit and scope of the invention.

Claims

1. A radial, tangential flow bottom for a chromatography column, characterized by: the sieve plate fixing device comprises a sieve plate (1), a fixing ring (2), a bus bar (3) and a flange plate (4), wherein the sieve plate (1) is fixed on the upper side of the sieve plate fixing ring (2), the lower side of the sieve plate fixing ring (2) is installed on the bus bar (3), and the flange plate (4) is installed below the bus bar (3); the novel screen plate is characterized in that a transverse upper flow channel (5) is formed between the bus plate (3) and the screen plate (1), a transverse lower flow channel (6) is formed between the bus plate (3) and the flange plate (4), a circle of vertical flow channel holes (10) are formed in the bus plate (3), and the flow channel holes (10) are communicated with the upper flow channel (5) and the lower flow channel (6).

2. A radial, tangential flow base for a chromatography column according to claim 1, wherein: the middle part of cylinder manifold (3) is upwards protruding, the solid fixed ring of sieve (2) cover is established and is installed on the bulge of cylinder manifold (3).

3. A radial, tangential flow base for a chromatography column according to claim 1, wherein: the upper surface of the bus board (3) inclines towards the center of the bus board and forms an inclined plane, and the upper surface of the flange plate (4) inclines towards the center of the bus board and forms an inclined plane.

4. A radial, tangential flow base for a chromatography column according to claim 3, wherein: the lower surface of the bus bar (3) is also inclined towards the center thereof and forms an inclined surface.

5. A radial, tangential flow base for a chromatography column according to claim 1, wherein: the bus bar (3) is provided with a plurality of circles of vertical balance holes (11), and the aperture of each balance hole (11) located on the outer circle is larger than that of each balance hole (11) located on the inner circle.

6. A radial, tangential flow base for a chromatography column according to claim 5, wherein: the diameters of the upper opening and the lower opening of the balance hole (11) are larger than that of the central part, and the longitudinal section of the balance hole is in a trapezoidal structure with the upper part and the lower part being symmetrical and the middle part being in a rectangular structure.

7. A chromatography system for use with the radial, tangential flow bottom for a chromatography column of any of claims 1-6, wherein: the screen plate is characterized by further comprising a spray head (7), a liquid inlet pipe (8) and a return pipe (9), wherein the spray head (7) sequentially penetrates through the centers of the flange plate (4), the bus bar (3) and the screen plate (1) from bottom to top, the spray head (7) is provided with a top cover (71), a side wall (72), a base (73), a valve rod (77), a baffle plate (78) and an air cylinder (79), a cavity (74) is formed among the top cover (71), the side wall (72) and the base (73), a through hole (75) is formed in the top of the side wall (72), and a groove (76) in the vertical direction is formed in the base (73);

the baffle plate (78) is arranged between the side wall (72) and the base (73), one side of the baffle plate (78) is fixed on the bus board (3), the other side of the baffle plate extends into the cavity (74), the top of the valve rod (77) protrudes to two sides and is positioned above the part of the baffle plate (78) extending to the cavity (74), and the air cylinder (79) is connected with the bottom of the valve rod (77).

8. A chromatography system according to claim 7, characterized in that: the top cover (71) is installed below the center of the sieve plate (1), the side wall (72) is installed at the center of the bus board (3), and the base (73) is installed in the middle of the flange plate (4).

9. A chromatography system according to claim 7, characterized in that: the liquid inlet pipe (8) penetrates through the base (73) to be communicated with the cavity (74), the upper flow channel (5) is communicated with the cavity (74) through the through hole (75), and the lower flow channel (6) is communicated with the return pipe (9) through the groove (76).

10. A chromatography system according to claim 7, characterized in that: the air cylinder (79) is fixedly arranged on the base (73), and a piston rod of the air cylinder is connected with the bottom of the valve rod (77).