CN114656738A - Biodegradable antibacterial film and preparation method thereof - Google Patents
Biodegradable antibacterial film and preparation method thereof Download PDFInfo
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- CN114656738A CN114656738A CN202210249907.6A CN202210249907A CN114656738A CN 114656738 A CN114656738 A CN 114656738A CN 202210249907 A CN202210249907 A CN 202210249907A CN 114656738 A CN114656738 A CN 114656738A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 13
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Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2329/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal, or ketal radical; Hydrolysed polymers of esters of unsaturated alcohols with saturated carboxylic acids; Derivatives of such polymer
- C08J2329/02—Homopolymers or copolymers of unsaturated alcohols
- C08J2329/04—Polyvinyl alcohol; Partially hydrolysed homopolymers or copolymers of esters of unsaturated alcohols with saturated carboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2401/00—Characterised by the use of cellulose, modified cellulose or cellulose derivatives
- C08J2401/02—Cellulose; Modified cellulose
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/07—Aldehydes; Ketones
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/15—Heterocyclic compounds having oxygen in the ring
- C08K5/151—Heterocyclic compounds having oxygen in the ring having one oxygen atom in the ring
- C08K5/1545—Six-membered rings
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- Manufacturing & Machinery (AREA)
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- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Manufacture Of Macromolecular Shaped Articles (AREA)
Abstract
The invention discloses a biodegradable antibacterial film and a preparation method thereof, belonging to the field of preparation of biodegradable antibacterial films. The prepared film has good mechanical property, water resistance, light transmittance, moisture retention property, bacteriostasis property and full biodegradation property, and solves the problems that the degradation rate of the antibacterial film is slow and the antibacterial film can not be completely degraded in the market, and the environment is polluted in the degradation process of the synthetic antibacterial agent.
Description
Technical Field
The invention belongs to the technical field of antibacterial film preparation, and particularly relates to a biodegradable antibacterial film and a preparation method thereof.
Background
With the increasing progress of the production level, the requirements of people on the antibacterial material are not limited to excellent antibacterial performance, and environment friendliness is also required. At the present stage, the popularization and use of non-plastic products, degradable shopping bags and packaging films (bags) are definitely proposed, and the scale popularization of the degradable mulching films is combined with the agricultural measures. At present, common antibacterial plastic films on the market are mostly prepared from traditional petroleum-based plastics such as PVC, PP, PE, PS and the like, are low in cost and easy to process and produce on a large scale, but cannot be completely and naturally degraded, and most of used antibacterial agents such as quaternary ammonium salts, pyridines, imidazoles, organic metals and the like have certain toxicity and are high in potential hazard to human bodies and the environment. The existing degradable film has the problems of difficult control of degradation rate, poor crack resistance, pollution to soil caused by residual film, damage to the structure of soil microbial flora and the like.
Disclosure of Invention
In order to overcome the disadvantages of the prior art, the present invention provides a biodegradable antibacterial film and a method for preparing the same
In order to achieve the purpose, the invention adopts the following technical scheme to realize the purpose:
the invention discloses a biodegradable antibacterial film, which comprises the following components: polyvinyl alcohol PVA, nanocellulose crystal CNC and tea polyphenol TP.
Preferably, the mass ratio of the polyvinyl alcohol PVA, the nano-cellulose crystal CNC and the tea polyphenol TP is 50 g: 10-70 g: 0.8-4 g.
The invention also discloses a preparation method of the biodegradable antibacterial film, which comprises the following steps:
the method comprises the following steps: preparing a material with the mass ratio of 50 g: (10-70) g: (0.8-4) g of polyvinyl alcohol PVA, nano cellulose crystal CNC and tea polyphenol TP;
step two: adding polyvinyl alcohol (PVA) into water to obtain a polyvinyl alcohol (PVA) aqueous solution with the concentration of 5%;
step three: adding 1-7% of nano-cellulose crystal CNC suspension into a polyvinyl alcohol PVA aqueous solution, and dripping a glutaraldehyde aqueous solution and glycerol to obtain a polyvinyl alcohol PVA/nano-cellulose crystal CNC blending solution;
step four: adding 0.08-0.4 g of tea polyphenol TP powder into a polyvinyl alcohol PVA/nano cellulose crystal CNC blending solution to prepare a membrane casting solution;
step five: and (3) forming the film casting solution on a polytetrafluoroethylene plate to obtain the antibacterial film.
Preferably, in the first step, the water bath is 95 ℃, the stirring speed is 700rpm, and the polyvinyl alcohol PVA aqueous solution is obtained after stirring and swelling for 1 h.
Preferably, in the second step, 1-2 ml of 2.5% glutaraldehyde aqueous solution and 1ml of glycerol are added dropwise.
Preferably, in the second step, after adding glutaraldehyde water solution and glycerol, stirring and dispersing at 700rpm for 1h to obtain polyvinyl alcohol PVA/nano cellulose crystal CNC blending solution.
Preferably, in the third step, after the polyvinyl alcohol PVA/nano cellulose crystal CNC blending solution is added into the tea polyphenol powder, stirring and dispersing are carried out for 30min at room temperature, the stirring speed is 700rpm, and stirring and dispersing are carried out for 30min, so as to prepare the membrane casting solution.
Preferably, in the third step, after preparing the membrane casting solution, standing at room temperature for defoaming, coating a polytetrafluoroethylene plate for film formation, standing at room temperature for 24 hours, and uncovering the film to obtain the antibacterial film.
Compared with the prior art, the invention has the following beneficial effects:
the invention discloses a biodegradable antibacterial film, which comprises polyvinyl alcohol PVA, nano cellulose crystal CNC and tea polyphenol TP, wherein the polyvinyl alcohol PVA is a biodegradable high-molecular polymer, has stable chemical property, high crystallinity, good thermal stability, hydrophilicity and film forming property, the nano cellulose crystal CNC is a natural cellulose extraction product, has the excellent performances of large surface area, high crystallinity, strong hydrophilicity, high strength/modulus, high rigidity, rich functional groups and the like, a large number of hydroxyl groups on the surface of the nano cellulose crystal CNC can generate intermolecular hydrogen bonds with the polyvinyl alcohol PVA to generate stronger interfacial interaction, the stability of a composite material is improved, the mechanical property and the water resistance of the polyvinyl alcohol PVA are enhanced, the tea polyphenol TP is a general term of polyphenol substances in tea leaves and is a natural antibacterial agent, and the main component of the nano cellulose crystal CNC comprises catechin antibacterial agents, Flavonoids, phenolic acids and the like, and has multiple functions of antibacterial property, antioxidation, cholesterol reduction, cancer resistance and the like. The polyvinyl alcohol PVA can be completely biodegraded under natural conditions, and the nano-cellulose crystal CNC and the tea polyphenol TP are reproducible natural organic antibacterial agents, so that the prepared antibacterial film is an ecological environment-friendly antibacterial film which has good biodegradation characteristics and good antibacterial property. The rigid nano filler nano cellulose crystal CNC has good compatibility with the polyvinyl alcohol PVA, and strong hydrogen bond action between the rigid nano filler nano cellulose crystal CNC and the polyvinyl alcohol PVA increases the crystallization capacity of the polyvinyl alcohol PVA film, and effectively improves the water resistance and the mechanical property of the polyvinyl alcohol PVA film; through the crosslinking action of glutaraldehyde, a compact and stable crosslinking network structure is formed among the molecular chains of the antibacterial film, so that on one hand, the mechanical property of the antibacterial film is improved, and the water resistance of the antibacterial film is increased; on the other hand, the air tightness of the antibacterial film is improved, and the heat preservation and soil moisture preservation capability of the antibacterial film is improved; the tea polyphenol TP has excellent natural antibacterial activity and oxidation resistance, and effectively improves the aging resistance of the film and prolongs the service life of the film while endowing the film with antibacterial and bacteriostatic properties.
Further, a polyvinyl alcohol PVA solution with the mass concentration of 5%, a nano cellulose crystal CNC suspension with the mass fraction of 1-7% and tea polyphenol TP powder with the mass of 0.08-0.4 g are adopted. The low-concentration polyvinyl alcohol PVA solution is beneficial to the uniform mixing of the casting solution, has good fluidity during casting film forming, and ensures the uniform thickness of the film. The too low content of the nano-crystalline CNC can result in unobvious reinforcing effect on the PVA film, and the too high content can result in the nano-crystalline CNC agglomerating in the PVA matrix, which affects the mechanical properties of the formed film. The antibacterial effect of the formed film is closely related to the content of the tea polyphenol TP, but excessive tea polyphenol can affect the physical and mechanical properties of the formed film, such as water vapor permeability, mechanical property and the like.
The invention adopts a tape casting film forming method to prepare a biodegradable antibacterial film. The polyvinyl alcohol PVA can be completely biodegraded under natural conditions, and the nano-cellulose crystal CNC and the tea polyphenol are reproducible natural organic antibacterial agents, so that the prepared antibacterial film is an ecological environment-friendly antibacterial film which has good biodegradation characteristics and good antibacterial property. The rigid nano filler nano cellulose crystal CNC has good compatibility with the polyvinyl alcohol PVA, and strong hydrogen bond action between the rigid nano filler nano cellulose crystal CNC and the polyvinyl alcohol PVA increases the crystallization capacity of the polyvinyl alcohol PVA film, and effectively improves the water resistance and the mechanical property of the polyvinyl alcohol PVA film; through the crosslinking action of glutaraldehyde, a compact and stable crosslinked network structure is formed among the molecular chains of the antibacterial film, so that on one hand, the mechanical property of the antibacterial film is improved, and the water resistance of the antibacterial film is increased; on the other hand, the air tightness of the antibacterial film is improved, and the heat preservation and soil moisture preservation capability of the antibacterial film is improved; the tea polyphenol TP has excellent natural antibacterial activity and oxidation resistance, and effectively improves the aging resistance of the film and prolongs the service life of the film while endowing the film with antibacterial and bacteriostatic properties. The biodegradable antibacterial film prepared by the invention has wide application prospect and commercial value in the fields of agriculture, packaging and the like.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the description and claims of the present invention and the terms first, second, etc. in the foregoing description are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in sequences other than those described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
The invention is described in further detail below:
a preparation method of a biodegradable antibacterial film comprises the following steps:
step 1: preparation of PVA/CNC blend solution: weighing 5g of PVA, adding the PVA into 100ml of deionized water, stirring the mixture for 1h at the stirring speed of 900rpm at the temperature of 95 ℃ in a water bath, and obtaining a PVA aqueous solution with the concentration of 5% (m/v); and continuously stirring and cooling to room temperature, adding 1-7% of CNC suspension into PVA mother liquor, dripping 1-2 ml of 2.5% glutaraldehyde water solution and 1ml of glycerol, and stirring and dispersing at 700rpm for 1h to obtain PVA/CNC blending solution.
And 2, step: preparing a PVA/CNC/antibacterial agent composite membrane: and (3) adding 0.08-0.4 g of tea polyphenol powder into the solution obtained in the first step, stirring and dispersing for 30min at room temperature, stirring at 700rpm, stirring and dispersing for 30min to prepare a membrane casting solution, standing for defoaming at room temperature, coating a clean polytetrafluoroethylene plate to form a membrane, standing for 24h at room temperature, and uncovering the membrane to obtain the antibacterial membrane.
The biodegradable antibacterial film is prepared by taking a biodegradable high polymer material as a base material and adding a natural organic antibacterial agent, is non-toxic, harmless, green and environment-friendly, and can be widely popularized and applied in the fields of food packaging, agricultural production and the like.
The polyvinyl alcohol PVA is a biodegradable high molecular polymer, the main chain is a carbon chain, a large number of hydroxyl groups are distributed on the side chain, hydrogen bonds are easy to form, the chemical property is stable, the crystallinity is high, and the polyvinyl alcohol PVA has good thermal stability, hydrophilicity and film forming property, and is widely used for synthesizing and preparing films and hydrogel. However, under a high humidity environment, the hydroxyl groups and water molecules form intermolecular hydrogen bonding, so that the structure of the polyvinyl alcohol PVA is damaged, and the water resistance of the polyvinyl alcohol PVA film is poor. Common methods for improving the water resistance of polyvinyl alcohol PVA include nano reinforcement, compounding plasticization, crosslinking modification, blending hybridization and the like. The nano reinforcement is simple to operate, and has positive influence on the improvement of the water resistance and the mechanical property of the polyvinyl alcohol PVA. The nano-cellulose crystal CNC is a natural cellulose extraction product, can be separated and prepared from natural organisms such as wood, cotton, algae and the like, has wide sources and is biodegradable. The nano-cellulose crystal CNC has the excellent performances of large surface area, high crystallinity, strong hydrophilicity, high strength/modulus, high rigidity, rich functional groups and the like, and a large number of hydroxyl groups on the surface of the CNC can generate intermolecular hydrogen bonds with the polyvinyl alcohol PVA to generate stronger interfacial interaction, so that the stability of the composite material is improved, and the mechanical property and the water resistance of the polyvinyl alcohol PVA are enhanced.
The natural antibacterial agent tea polyphenol TP is a general term of polyphenol substances in tea, mainly comprises catechins, flavonoids, phenolic acids and the like, and has multiple functions of antibacterial property, antioxidation, cholesterol reduction, cancer resistance and the like. The tea polyphenol can inhibit the growth and the multiplication of microorganisms by changing the cell morphology of thalli, hindering the generation of bacterial cell walls, destroying the integrity of cell membranes, influencing the cell enzyme activity, DNA and the like, and has good bacteriostatic activity on escherichia coli, staphylococcus aureus, candida albicans and the like. Because of high safety, low cost, no toxicity and rich sources, the research on the application and the health care mechanism of the tea polyphenol relates to a plurality of fields of food, daily chemicals, medicine and the like.
The invention utilizes the CNC of the natural nano-cellulose crystal to improve the physical and mechanical properties of the PVA film, and the natural antibacterial agent tea polyphenol endows the film with excellent antibacterial property. Provides a preparation method of the biodegradable antibacterial film, and provides reference for developing green and environment-friendly biodegradable antibacterial film materials.
Example 1: agricultural mulching film
Step 1: preparation of polyvinyl alcohol PVA solution: weighing 5g of polyvinyl alcohol PVA, adding the polyvinyl alcohol PVA into 100ml of deionized water, stirring for swelling for 1h at the stirring speed of 700rpm in a water bath at 95 ℃, and continuously stirring and cooling to room temperature to obtain a polyvinyl alcohol PVA aqueous solution with the concentration of 5% (m/v).
And 2, step: preparing polyvinyl alcohol PVA/nano cellulose crystal CNC (computer numerical control) blending liquid: adding 3.43g of nano-cellulose crystal CNC suspension with the mass fraction of 7 wt% into polyvinyl alcohol PVA mother liquor, dripping 1ml of glutaraldehyde aqueous solution with the mass fraction of 2.5% and 1ml of glycerol, stirring and dispersing at 700rpm for 30min to obtain polyvinyl alcohol PVA/nano-cellulose crystal CNC blended solution.
And step 3: preparing a polyvinyl alcohol PVA/nano cellulose crystal CNC/tea polyphenol TP composite membrane: adding 0.08gTP into the polyvinyl alcohol PVA/nano-cellulose crystal CNC blended solution, stirring and dispersing for 30min at room temperature with the stirring speed of 700rpm to prepare a casting solution, standing and defoaming at room temperature, coating a wire rod with the diameter of 100 mu m of a film scraper on a clean polytetrafluoroethylene plate at the speed of 15mm/min to form a film, standing for 24h at room temperature, and uncovering the film to obtain the agricultural biodegradable polyvinyl alcohol PVA/nano-cellulose crystal CNC/tea polyphenol TP antibacterial mulching film.
The polyvinyl alcohol PVA/nano cellulose crystal CNC/tea polyphenol TP agricultural biodegradable antibacterial mulching film prepared by the method meets the II-class biodegradable agricultural mulching film specified in the full-biodegradable agricultural ground covering film (GB/T35795 and 2017), the thickness of the mulching film is 0.032 +/-0.003 mm, the breaking strength is 67 +/-5 MPa, the breaking elongation is 246.36 +/-12.73%, the light transmittance is 83.26 +/-0.75%, and the water vapor transmission rate is 1765.7 +/-36.4 g/(m)224h), the inhibition rate of the mulching film on escherichia coli is 94.35%, the inhibition rate of the mulching film on staphylococcus aureus is 95.38%, the inhibition rate of the mulching film on candida albicans is 83.64%, the service life is about 160 days, and the mulching film is suitable for replacing incompletely biodegradable agricultural mulching films and non-degradable agricultural mulching films.
Example 2: antibacterial packaging film
Step 1: preparation of polyvinyl alcohol PVA solution: weighing 5g of polyvinyl alcohol PVA, adding the polyvinyl alcohol PVA into 100ml of deionized water, stirring for swelling for 1h at the stirring speed of 700rpm in a water bath at 95 ℃, and continuously stirring and cooling to room temperature to obtain a polyvinyl alcohol PVA aqueous solution with the concentration of 5% (m/v).
Step 2: preparing polyvinyl alcohol PVA/nano cellulose crystal CNC (computer numerical control) blending liquid: adding 1.1g of nano-cellulose crystal CNC suspension with the mass fraction of 7 wt% into polyvinyl alcohol PVA mother liquor, dripping 2ml of glutaraldehyde aqueous solution with the mass fraction of 2.5% and 1ml of glycerol, stirring and dispersing at 700rpm for 30min to obtain polyvinyl alcohol PVA/nano-cellulose crystal CNC blended solution.
And step 3: preparing a polyvinyl alcohol PVA/nano cellulose crystal CNC/tea polyphenol TP composite film: adding 0.32gTP into PVA/CNC blended liquid, stirring and dispersing for 30min at room temperature with the stirring speed of 700rpm to prepare a casting solution, standing for defoaming at room temperature, coating a clean polytetrafluoroethylene plate with a 200-micron wire bar of a film scraper at the speed of 20mm/min to form a film, standing for 24h at room temperature, and uncovering the film to obtain the biodegradable polyvinyl alcohol PVA/nano cellulose crystal CNC/tea polyphenol TP antibacterial film for packaging.
The antibacterial packaging biodegradable polyvinyl alcohol PVA/nano cellulose crystal CNC/tea polyphenol TP antibacterial film prepared by the method meets the packaging plastic film and bag for express delivery and delivery of full biodegradable logistics (GB/T38727-224h), the bacteriostasis rate to escherichia coli is 96.35%, the bacteriostasis rate to staphylococcus aureus is 96.27%, the bacteriostasis rate to candida albicans is 84.49%, the complete degradation period is about 160%, and the composite material is suitable for replacing the existing non-biodegradable plastic packaging material.
The biodegradable antibacterial films prepared in step 3 in examples 1 and 2 were tested for breaking strength, elongation at break and bacteriostasis rate, and the specific test results are shown in tables 1 and 2.
TABLE 1 tables of breaking Strength and elongation at Break test data
| Breaking Strength (MPa) | Elongation at Break (%) | |
| 1.1gCNC | 45±5 | 164.34±15.64 |
| 3.43gCNC | 67±5 | 246.36±12.73 |
TABLE 2 antibacterial Property test data sheet
It can be seen from tables 1 and 2 that, with the increase of the contents of the nano-cellulose crystal CNC and the tea polyphenol TP, the breaking strength and the breaking elongation of the polyvinyl alcohol PVA/nano-cellulose crystal CNC/tea polyphenol TP antibacterial film and the bacteriostatic rates of staphylococcus aureus, escherichia coli and candida albicans are all significantly improved, and it is proved that the mechanical property and the antibacterial property of the polyvinyl alcohol PVA/nano-cellulose crystal CNC/tea polyphenol TP antibacterial film can be enhanced by the increase of the proportion of the nano-cellulose crystal CNC and the tea polyphenol TP.
The above-mentioned contents are only for illustrating the technical idea of the present invention, and the protection scope of the present invention is not limited thereby, and any modification made on the basis of the technical idea of the present invention falls within the protection scope of the claims of the present invention.
Claims (8)
1. A biodegradable antibacterial film is characterized by comprising the following components: polyvinyl alcohol PVA, nanocellulose crystal CNC and tea polyphenol TP.
2. The biodegradable antibacterial film as claimed in claim 1, wherein the mass ratio of the polyvinyl alcohol PVA, the nano cellulose crystal CNC and the tea polyphenol TP is 50 g: (10-70) g: (0.8-4) g.
3. The method for preparing a biodegradable antibacterial film according to any one of claims 1 to 2, comprising:
the method comprises the following steps: preparing a mixture with the mass ratio of 50 g: (10-70) g: (0.8-4) g of polyvinyl alcohol PVA, nano cellulose crystal CNC and tea polyphenol TP;
step two: adding polyvinyl alcohol (PVA) into water to obtain a polyvinyl alcohol (PVA) aqueous solution with the concentration of 5%;
step three: adding 1-7% of nano-cellulose crystal CNC suspension into a polyvinyl alcohol PVA aqueous solution, and dripping a glutaraldehyde aqueous solution and glycerol to obtain a polyvinyl alcohol PVA/nano-cellulose crystal CNC blending solution;
step four: adding 0.08-0.4 g of tea polyphenol TP powder into a polyvinyl alcohol PVA/nano cellulose crystal CNC blending solution to prepare a membrane casting solution;
step five: and (3) forming the film casting solution on a polytetrafluoroethylene plate to obtain the antibacterial film.
4. The method for preparing a biodegradable antibacterial film according to claim 3, wherein in the first step, the polyvinyl alcohol PVA aqueous solution is obtained by stirring and swelling in a water bath at 95 ℃ and a stirring speed of 700rpm for 1 hour.
5. The method for preparing a biodegradable antibacterial film according to claim 3, characterized in that in the second step, 1-2 ml of 2.5% glutaraldehyde solution and 1ml of glycerol are added dropwise.
6. The method for preparing a biodegradable antibacterial film according to claim 3, wherein in the second step, after adding glutaraldehyde aqueous solution and glycerol, stirring at 700rpm for dispersing for 1h to obtain polyvinyl alcohol PVA/nano cellulose crystal CNC blending solution.
7. The preparation method of the biodegradable antibacterial film according to claim 3, characterized in that in the third step, after the polyvinyl alcohol PVA/nanocellulose crystal CNC blending solution is added to the tea polyphenol powder, the mixture is stirred and dispersed for 30min at room temperature, the stirring speed is 700rpm, and the mixture is stirred and dispersed for 30min to prepare the casting solution.
8. The method for preparing a biodegradable antibacterial film according to claim 3, wherein in the third step, after preparing the casting solution, standing at room temperature for defoaming, coating a polytetrafluoroethylene plate to form a film, standing at room temperature for 24h, and peeling off the film to obtain the antibacterial film.
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