CN114653093B - Extraction and purification device of polysaccharide - Google Patents
Extraction and purification device of polysaccharide Download PDFInfo
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- CN114653093B CN114653093B CN202210409278.9A CN202210409278A CN114653093B CN 114653093 B CN114653093 B CN 114653093B CN 202210409278 A CN202210409278 A CN 202210409278A CN 114653093 B CN114653093 B CN 114653093B
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- 238000000605 extraction Methods 0.000 title claims abstract description 71
- 238000000746 purification Methods 0.000 title claims abstract description 47
- 150000004676 glycans Chemical class 0.000 title claims abstract description 33
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 33
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 33
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 239000012528 membrane Substances 0.000 claims abstract description 13
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 6
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 20
- 239000002904 solvent Substances 0.000 claims description 18
- 238000004440 column chromatography Methods 0.000 claims description 15
- 238000000926 separation method Methods 0.000 claims description 15
- 230000009471 action Effects 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 13
- 239000000843 powder Substances 0.000 description 13
- 230000008569 process Effects 0.000 description 10
- 238000001914 filtration Methods 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000012856 packing Methods 0.000 description 4
- 238000002386 leaching Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005485 electric heating Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000007790 scraping Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000007976 Ketosis Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 150000001323 aldoses Chemical class 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 150000002584 ketoses Chemical class 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0261—Solvent extraction of solids comprising vibrating mechanisms, e.g. mechanical, acoustical
- B01D11/0265—Applying ultrasound
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D1/00—Evaporating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/087—Cleaning containers, e.g. tanks by methods involving the use of tools, e.g. brushes, scrapers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B30—PRESSES
- B30B—PRESSES IN GENERAL
- B30B9/00—Presses specially adapted for particular purposes
- B30B9/02—Presses specially adapted for particular purposes for squeezing-out liquid from liquid-containing material, e.g. juice from fruits, oil from oil-containing material
- B30B9/04—Presses specially adapted for particular purposes for squeezing-out liquid from liquid-containing material, e.g. juice from fruits, oil from oil-containing material using press rams
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mechanical Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Acoustics & Sound (AREA)
- Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses a polysaccharide extraction and purification device, and belongs to the technical field of compound extraction devices. The device comprises an extraction tank, wherein a tank cover is arranged at the top end of the extraction tank, a lifting device is arranged in the extraction tank, the lifting device comprises a telescopic component and a telescopic plate, one end of the telescopic component is connected with the telescopic plate, and the other end of the telescopic component penetrates through the tank cover and is connected with a motor component; the inner wall of the extraction tank is provided with an ultrasonic oscillator, a sensing assembly and a containing plate; the outer wall of the extraction tank is provided with a refrigerator; the top surface of the concentration tank is communicated with the bottom surface of the extraction tank through a filter membrane; the concentrating tank is connected with the extracting tank through a communicating pipe, the side surface of the concentrating tank is provided with an inlet and an outlet, and a heating component is arranged in the concentrating tank; and the purification tank is arranged at the lower end of the extraction tank and is arranged on one side of the concentration tank, and a purification chromatography device is arranged in the purification tank. The extraction and purification device for polysaccharide provided by the invention can avoid the problem of low extraction rate caused by long-time extraction of raw materials floating on the extracting solution.
Description
Technical Field
The invention relates to the technical field of compound extraction devices, in particular to a polysaccharide extraction and purification device.
Background
Polysaccharides are natural high molecular polymers formed by connecting aldose or ketose through glycosidic bond, are important biological macromolecules in organisms, and are one of basic substances for maintaining normal operation of vital activities. Polysaccharides are ubiquitous in plants in nature. Many plant polysaccharides have biological activity, and have health promotion effects including regulating immunity, lowering blood sugar, reducing blood lipid, resisting radiation, resisting bacteria and virus, and protecting liver.
Based on this, researchers have installed a device for extracting polysaccharides. In the process of extracting polysaccharide, the extraction time is too long, and the effective components are easy to change under the condition that the liquid medicine is heated for a long time, so that the extraction rate is reduced. However, the plants often float on the upper layer of the extract liquid during the extraction process, and a long time is required for the plants to settle into the extract liquid by themselves, thereby causing a decrease in the extraction rate.
Disclosure of Invention
Aiming at the problems, the invention provides a device for extracting and purifying polysaccharide, which avoids the problem of low extraction rate caused by long-time extraction of raw materials floating on an extracting solution.
The invention aims to provide a polysaccharide extraction and purification device, which comprises:
the top end of the extraction tank is provided with a tank cover, a lifting device is arranged in the extraction tank and comprises a telescopic assembly and a telescopic plate, one end of the telescopic assembly is connected with the telescopic plate, and the other end of the telescopic assembly penetrates through the tank cover and is connected with a motor assembly; the inner wall of the extraction tank is provided with an ultrasonic oscillator, a sensing assembly and a containing plate; a refrigerator is arranged on the outer wall of the extraction tank; during extraction, the raw materials are placed in the extracting solution through the telescopic plate, and after the raw materials can be placed in the extracting solution by self, the telescopic plate scrapes walls and stirs under the action of the telescopic assembly;
the concentration tank is arranged at the lower end of the extraction tank, and the top surface of the concentration tank is communicated with the bottom surface of the extraction tank through a filtering membrane; the concentration tank is connected with the extraction tank through a communicating pipe, the side surface of the concentration tank is also provided with an inlet and an outlet, and a heating assembly is arranged in the concentration tank;
the purification jar, its set up in the lower extreme of drawing the jar, and locate one side of concentrated jar, be equipped with purification chromatography device in the purification jar.
Preferably, the telescopic plate comprises a first fixed plate, a first extending plate, a second extending plate, a first rotating plate and a second rotating plate, one end of the first fixed plate is connected with one end of the first extending plate through an elastic assembly, and the other end of the first fixed plate is connected with the second extending plate through another elastic assembly; the telescopic assembly comprises a first extending plate, a second extending plate, a first fixing plate, a second rotating plate, a first rotating plate, a second rotating plate, a telescopic assembly and a second rotating plate, wherein the other end of the first extending plate is rotatably connected with one end of the first rotating plate, the other end of the first rotating plate is rotatably connected with one end of the second rotating plate, the other end of the second rotating plate is rotatably connected with the other end of the second extending plate, and the rotating connection positions of the first fixing plate, the first rotating plate and the second rotating plate are connected with the telescopic assembly.
Preferably, the telescopic assembly comprises a first telescopic rod and a second telescopic rod, the first telescopic rod is connected with the first fixing plate, and the second telescopic rod is connected with the rotating connection position of the first rotating plate and the second rotating plate.
Preferably, a first controller is further arranged in the extraction tank, and the first controller is respectively connected with the sensing assembly, the ultrasonic oscillator, the first motor, the first switch and the first power supply.
Preferably, purification chromatographic device includes column chromatography separation post, solvent ball and pressure device, solvent ball and column chromatography separation post are from the top down in proper order and are equipped with in the purification jar.
Preferably, the pressurizing device comprises an inflatable ball and an inflatable tube, one end of the inflatable ball is connected with one end of the inflatable tube, and the other end of the inflatable tube is connected with the solvent ball.
Preferably, pressure device still includes fixing device, fixing device includes fixed frame, second fixed plate and third telescopic link, the one end of third telescopic link with fixed frame is connected, the other end of third telescopic link with the second fixed plate is connected, the second fixed plate with fixed frame sliding connection, the second fixed plate with keep away from the cover is equipped with between the fixed frame of third telescopic link aerify the ball.
Preferably, still be equipped with sample box and liquid case on the purification jar, the sample box with column chromatography separation column intercommunication, the liquid case with solvent ball intercommunication, the sample box with all be equipped with the valve on the liquid case.
Preferably, the purification tank is further provided with a second controller, and the second controller is connected with the third telescopic rod, the valve on the sample tank and the liquid tank, the second switch and a second power supply.
Compared with the prior art, the invention has the following beneficial effects:
the polysaccharide is obtained through extraction, concentration and purification, in the extraction step, in order to avoid floating of the raw materials on the extracting solution, the telescopic plate is arranged in the extraction tank, the raw materials are extracted in the extracting solution through the telescopic plate, when the raw materials can still be in the extracting solution without passing through the telescopic plate, the telescopic plate is triangular, the wall scraping can be performed, the raw materials are prevented from being adhered in the extraction tank, meanwhile, the stirring effect can be achieved while the wall scraping is performed, and in the ultrasonic extraction process, the stirring treatment is assisted, so that the extraction is more efficient;
in order to enable the telescopic plate to scrape the wall, the second telescopic rod moves downwards, then the first rotating plate and the second rotating plate are driven to rotate to be V-shaped, and then the first extending plate and the second extending plate contract under the action of the two elastic assemblies, so that the telescopic plate is triangular to scrape the wall and stir;
after extraction is finished, the first rotating plate, the second rotating plate, the first extending plate and the second extending plate are reset through the second telescopic rod, then the telescopic plates move downwards under the action of the first telescopic rod, and extracted raw material powder is extruded under the action of the telescopic plates and the object containing plates, so that waste caused by extraction liquid remaining in the extracted raw material powder is avoided; after the extracting solution completely enters the concentration tank, concentration treatment is started, and in the concentration process, the evaporated solvent enters the extraction tank through the communicating pipe so as to achieve the recycling of the solvent and reduce the waste of the solvent.
After the concentrated extracting solution is precipitated by ethanol, centrifugal drying treatment is carried out, the extracting solution is transferred into a purification tank for purification treatment, a pressurizing device is further arranged in the purification process, and the inflatable ball can be pressurized in the process of extension or shortening of the third telescopic rod, so that the purification time is shortened, and the purification efficiency is improved.
Drawings
FIG. 1 is a schematic structural diagram of a polysaccharide extraction and purification device provided by the present invention;
FIG. 2 is a schematic structural view of a retractable plate according to the present invention;
FIG. 3 is a side view of the purification tank provided by the present invention;
fig. 4 is a schematic structural diagram of a fixing device provided by the present invention.
Reference numerals:
1-an extraction tank, 11-a tank cover, 12-a telescopic plate, 121-a first fixing plate, 122-a first elongated plate, 123-a second elongated plate, 124-a first rotating plate, 125-a second rotating plate, 13-a motor component, 14-an ultrasonic oscillator, 15-a refrigerator, 16-a containing plate, 2-a concentration tank, 21-a filter membrane, 22-a communicating pipe, 3-a purification tank, 31-a column chromatography separation column, 32-a solvent ball, 33-an inflation ball, 34-an inflation pipe, 35-a fixing frame, 36-a second fixing plate, 37-a third telescopic rod, 38-a sample box and 39-a liquid box.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a device for extracting and purifying polysaccharide, which is shown in figures 1-4 and comprises: the extraction tank 1 is used for extracting polysaccharide, a tank cover 11 is arranged at the top end of the extraction tank 1, a lifting device is arranged in the extraction tank 1, the lifting device comprises a telescopic component and a telescopic plate 12, one end of the telescopic component is connected with the telescopic plate 12, and the other end of the telescopic component penetrates through the tank cover 11 and is connected with a motor component 13; specifically, the telescopic plate 12 includes a first fixed plate 121, a first elongated plate 122, a second elongated plate 123, a first rotating plate 124 and a second rotating plate 125, wherein one end of the first fixed plate 121 is connected to one end of the first elongated plate 122 through an elastic member, and the other end of the first fixed plate 121 is connected to the second elongated plate 123 through another elastic member; the other end of the first elongated plate 122 is rotatably coupled to one end of the first rotating plate 124, the other end of the first rotating plate 124 is rotatably coupled to one end of the second rotating plate 125, and the other end of the second rotating plate 125 is rotatably coupled to the other end of the second elongated plate 123; the two elastic components are both springs; the telescopic assembly is connected to the pivotal connection points of the first fixed plate 121, the first pivotal plate 124 and the second pivotal plate 125. Further, the telescopic assembly comprises a first telescopic rod and a second telescopic rod, the first telescopic rod is connected with the first fixing plate 121, and the second telescopic rod is connected with the rotating connection position of the first rotating plate 124 and the second rotating plate 125; the motor assembly 13 is a rotary motor for controlling the rotation of the expansion plate 12. During extraction, the raw materials are placed in the extracting solution through the telescopic plate, and after the raw materials can be placed in the extracting solution by themselves, the telescopic plate scrapes walls and stirs under the action of the telescopic assembly; specifically, firstly, the plant is crushed to obtain plant powder, and when the plant is used for extracting polysaccharide, the plant powder tends to float on the upper layer of the extracting solution, therefore, in order to avoid the plant powder floating on the upper layer of the extracting solution, the telescopic plate 12 is provided, the first fixed plate 121, the first elongated plate 122, the second elongated plate 123, the first rotating plate 124 and the second rotating plate 125 in the telescopic plate 12 are all filtering membranes, the meshes of the filtering membranes are smaller than those of the plant powder, so that the plant powder is prevented from passing through the filtering membrane to float on the upper layer of the extracting solution, when the pot cover 11 is used, the first telescopic rod is controlled to move downwards until the plant powder is in the extracting solution, so that the polysaccharide can be better extracted, when the plant powder can still be in the extracting solution without the action of the telescopic plate 12 in the extracting process, the second telescopic rod is moved downwards, the first rotating plate 124 and the second rotating plate 125 are in a V shape during the movement, the first extending plate 122 and the second extending plate 123 respectively slide into the first fixing plate 121 under the action of the elastic assembly during the rotation of the first rotating plate 124 and the second rotating plate 125, and further the length of two sides of the telescopic plate 12 is shortened, then, under the control of the motor assembly 13, the other pair of opposite sides of the telescopic plate 12 rotate along the wall of the extraction tank 1, so that the plant powder attached to the wall of the extraction tank 1 during the extraction process can be effectively scraped, and meanwhile, a stirring function can be assisted, so that the extraction is more efficient. In addition, the inner wall of the extraction tank 1 is provided with an ultrasonic oscillator 14, a sensing assembly and an object holding plate 16; sensing element is level sensor and temperature sensor, flourishing thing board 16 is located draw the bottom of jar 1, flourishing thing board 16 is filtration membrane, filtration membrane's mesh is less than the plant powder, through telescopic link 12 with flourishing thing board 16's interact extrudees the plant powder after having drawed. The refrigerator 15 is arranged on the outer wall of the extraction tank 1 and used for adjusting the temperature in the extraction tank 1 in the extraction process and the temperature in the extraction tank 1 in the concentration process; level sensor is used for detecting the addition of extract, when waiting to add the position of settlement, stops adding the extract, utilizes ultrasonic oscillator 14 carries out the supersound to plant powder and draws, at the in-process that the supersound drawed, utilizes temperature sensor detects draw the temperature in the jar 1, if the temperature is too high, temperature sensor signals, refrigerator 15 carries out refrigeration treatment, ensures to draw the in-process at the supersound, the temperature in jar 1 of drawing is in suitable temperature, avoids drawing the influence that causes because of the high temperature to the polysaccharide.
Still be equipped with first controller in the jar 1 of extracting, first controller respectively with level sensor temperature sensor ultrasonic oscillator 14 motor element 13, first switch and first power are connected. It should be noted that, the speeds and distances of the upward movement and the downward movement of the first telescopic rod and the second telescopic rod are written in the first controller in advance, when the controller works, the distances of the movement of the first telescopic rod and the second telescopic rod are constant, and the rotational speed of the telescopic plate 12 driven by the second motor is written in the controller in advance, that is, the rotational speed of the telescopic plate 12 is constant.
After the extraction of the polysaccharide is completed, in order to make the extracting solution flow into a concentration tank 2, a concentration step is carried out, wherein the concentration tank 2 is arranged at the lower end of the extracting tank 1, and the top surface of the concentration tank 2 is communicated with the bottom surface of the extracting tank 1 through a filter membrane 21; the concentrating tank 2 is connected with the extracting tank 1 through a communicating pipe 22, an inlet and an outlet are further formed in the side face of the concentrating tank 2, a heating assembly is arranged in the concentrating tank 2, and the heating assembly is an electric heating pipe. Firstly, the switch of the filtering membrane 21 is turned on to enable the extracting solution to flow into the concentration tank 2 from the extraction tank 1, then the second telescopic rod moves upwards to enable the first rotating plate 124 and the second rotating plate 125 to be in a straight line shape, then the first telescopic rod continues to extend to enable the telescopic plate 12 to move downwards, and the extracting solution flows into the concentration tank 2 through the filtering membrane 21 by extrusion of the telescopic plate 12 and the containing plate 16, so that waste caused by extracting solution residue is avoided. After all the extract flows into the concentration tank 2, the switch of the filter membrane 21 is closed to start concentration treatment, the temperature in the concentration tank 2 is raised to a set temperature by the electric heating tube, and then the temperature in the extraction tank 1 is lowered to a temperature at which steam of the extract can be condensed by the refrigerator 15. And evaporation and concentration are carried out, and the evaporated solution enters the extraction tank 1 through the communicating pipe 22 in the evaporation process of the extracting solution, so that the recycling of the solvent is realized. Concentrating the extracting solution to 1/8 of the extracting solution, finishing the concentrating step, adding ethanol through the inlet for precipitation treatment, discharging through the outlet after the ethanol and the concentrated extracting solution are uniformly mixed, performing centrifugal treatment, removing supernatant, drying the precipitate to obtain crude polysaccharide, and then performing purification treatment.
The purification of crude polysaccharide is handled through purification jar 3, purification jar 3 set up in draw jar 1 lower extreme, and locate one side of concentrated jar 2, be equipped with purification chromatography device in the purification jar 3. And carrying out purification treatment by the purification chromatography device. Purification chromatographic device includes column chromatography separation post 31, solvent ball 32 and pressure device, solvent ball 32 and column chromatography separation post 31 are equipped with from the top down in proper order in purification jar 3. Further, still be equipped with sample case 38 and liquid case 39 on the purification jar 3, sample case 38 with column chromatography separation column 31 communicates, liquid case 39 with solvent ball 32 communicates, sample case 38 with all be equipped with the valve on the liquid case 39. The crude polysaccharide is first dissolved in a solvent and then placed in the sample tank 38, and the liquid tank 39 contains an eluent. After the column chromatography separation column 31 is packed (the column packing method is dry column packing or wet column packing), a valve on the sample box 38 is opened, so that the dissolved crude polysaccharide is placed in the column chromatography separation column 31, then, the valve on the liquid box 39 is opened to carry out leaching, and a pressurizing device is used in the leaching process to enable a leaching solution to "walk the column", specifically, the pressurizing device comprises an inflatable ball 33 and an inflatable tube 34, one end of the inflatable ball 33 is connected with one end of the inflatable tube 34, and the other end of the inflatable tube 34 is connected with the solvent ball 32. Further, pressure device still includes fixing device, fixing device includes fixed frame 35, second fixed plate 36 and third telescopic link 37, the one end of third telescopic link 37 with fixed frame 35 is connected, the other end of third telescopic link 37 with second fixed plate 36 is connected, second fixed plate 36 with fixed frame 35 sliding connection, second fixed plate 36 with keep away from the cover is equipped with between the fixed frame 35 of third telescopic link 37 aerify ball 33. During the pressurization process, the third expansion link 37 moves up and down, so that the second fixing plate 36 slides in the fixing frame 35, and the inflation ball 33 is pressed, thereby performing the pressurization process.
The purification tank 3 is further provided with a second controller, and the second controller is connected with the third telescopic rod 37, the valve on the sample tank 38 and the liquid tank 39, the second switch and a second power supply. It should be noted that the speed and distance of the upward and downward movement of the third telescopic rod 37 are written in the second controller in advance. When carrying out purification processing, after 31 packing of column chromatography separation column was accomplished, open valve on the sample case 38 will dissolve the crude polysaccharide solution after add in 31 in the column chromatography separation column, then will open valve on the liquid case 39, add the leacheate under the effect of telescopic link 37 for the leacheate has carried out the post, then opens the switch of column chromatography separation column 31, collects and obtains the polysaccharide after the purification.
It should be noted that, the connection relationship of the components not specifically mentioned in the present invention is the default of the prior art, and the connection relationship of the structure is not described in detail since it does not relate to the invention point and is a common application of the prior art.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including the preferred embodiment and all changes and modifications that fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (7)
1. An extraction and purification device of polysaccharide is characterized by comprising:
the extraction tank (1) is provided with a tank cover (11) at the top end, a lifting device is arranged in the extraction tank (1), the lifting device comprises a telescopic component and a telescopic plate (12), one end of the telescopic component is connected with the telescopic plate (12), and the other end of the telescopic component penetrates through the tank cover (11) and is connected with a motor component (13); the inner wall of the extraction tank (1) is provided with an ultrasonic oscillator (14), a sensing assembly and a containing plate (16); a refrigerator (15) is arranged on the outer wall of the extraction tank (1); during extraction, the raw materials are placed in the extracting solution through the telescopic plate (12), and after the raw materials can be placed in the extracting solution by themselves, the telescopic plate (12) scrapes walls and stirs under the action of the telescopic assembly;
the retractable plate (12) comprises a first fixed plate (121), a first elongated plate (122), a second elongated plate (123), a first rotating plate (124) and a second rotating plate (125), one end of the first fixed plate (121) is connected with one end of the first elongated plate (122) through an elastic assembly, and the other end of the first fixed plate (121) is connected with the second elongated plate (123) through another elastic assembly; the other end of the first extension plate (122) is rotatably connected with one end of the first rotation plate (124), the other end of the first rotation plate (124) is rotatably connected with one end of the second rotation plate (125), the other end of the second rotation plate (125) is rotatably connected with the other end of the second extension plate (123), and the rotating joints of the first fixing plate (121), the first rotation plate (124) and the second rotation plate (125) are all connected with the telescopic assembly;
the telescopic assembly comprises a first telescopic rod and a second telescopic rod, the first telescopic rod is connected with the first fixing plate (121), and the second telescopic rod is connected with the rotating connection position of the first rotating plate (124) and the second rotating plate (125);
a concentration tank (2) which is arranged at the lower end of the extraction tank (1), wherein the top surface of the concentration tank (2) is communicated with the bottom surface of the extraction tank (1) through a filter membrane (21); the concentration tank (2) is connected with the extraction tank (1) through a communicating pipe (22), an inlet and an outlet are arranged on the side surface of the concentration tank (2), and a heating assembly is arranged in the concentration tank (2);
a purification tank (3) disposed at the lower end of the extraction tank (1) and disposed at one side of the concentration tank (2), wherein a purification chromatography device is disposed in the purification tank (3).
2. The polysaccharide extracting and purifying device according to claim 1, wherein a first controller is further disposed in the extracting tank (1), and the first controller is respectively connected to the sensing assembly, the ultrasonic oscillator (14), the motor assembly (13), the first switch and the first power supply.
3. The polysaccharide extraction and purification device as claimed in claim 1, wherein the purification and chromatography device comprises a column chromatography separation column (31), a solvent ball (32) and a pressurizing device, and the pressurizing device, the solvent ball (32) and the column chromatography separation column (31) are sequentially arranged in the purification tank (3) from top to bottom.
4. The polysaccharide extracting and purifying device according to claim 3, wherein the pressurizing device comprises an air-filled ball (33) and an air-filled tube (34), one end of the air-filled ball (33) is connected with one end of the air-filled tube (34), and the other end of the air-filled tube (34) is connected with the solvent ball (32).
5. The polysaccharide extraction and purification device according to claim 4, wherein the pressurizing device further comprises a fixing device, the fixing device comprises a fixing frame (35), a second fixing plate (36) and a third telescopic rod (37), one end of the third telescopic rod (37) is connected with the fixing frame (35), the other end of the third telescopic rod (37) is connected with the second fixing plate (36), the second fixing plate (36) is slidably connected with the fixing frame (35), and the inflatable ball (33) is sleeved between the second fixing plate (36) and the fixing frame (35) far away from the third telescopic rod (37).
6. The polysaccharide extracting and purifying device according to claim 5, wherein a sample tank (38) and a liquid tank (39) are further disposed on the purifying tank (3), the sample tank (38) is communicated with the column chromatography separation column (31), the liquid tank (39) is communicated with the solvent ball (32), and valves are disposed on the sample tank (38) and the liquid tank (39).
7. The polysaccharide extracting and purifying device according to claim 6, wherein a second controller is further disposed on the purifying tank (3), and the second controller is connected to the third telescopic rod (37), the valve, the second switch and the second power source on the sample tank (38) and the liquid tank (39).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210409278.9A CN114653093B (en) | 2022-04-19 | 2022-04-19 | Extraction and purification device of polysaccharide |
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