CN114618599A - Heating temperature control device and micro-fluidic system - Google Patents
Heating temperature control device and micro-fluidic system Download PDFInfo
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- CN114618599A CN114618599A CN202011470307.XA CN202011470307A CN114618599A CN 114618599 A CN114618599 A CN 114618599A CN 202011470307 A CN202011470307 A CN 202011470307A CN 114618599 A CN114618599 A CN 114618599A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
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Abstract
The utility model provides a heating temperature regulating device and micro-fluidic system belongs to micro-fluidic technical field, and it can solve the inhomogeneous problem of reaction liquid heating among the current micro-fluidic chip. The heating temperature control device of this disclosure includes: the device comprises a first substrate, a second substrate and a runner cavity layer, wherein the first substrate and the second substrate are oppositely arranged, and the runner cavity layer is positioned between the first substrate and the second substrate; the runner cavity layer includes: a plurality of first liquid storage tanks, a plurality of second liquid storage tanks, a plurality of first flow channels and a plurality of second flow channels; an orthographic projection of a first reservoir on the substrate at least partially overlaps an orthographic projection of a first heating element on the substrate; the first end of a first flow passage is communicated with a first liquid storage tank, and the second end of the first flow passage is communicated with a second liquid storage tank; the first end of a second flow passage is communicated with a first liquid storage tank, and the second end of the second flow passage is communicated with a through hole; the orthographic projection of the placing groove on the substrate is at least partially overlapped with the orthographic projection of the second liquid storage tank on the substrate; the switching element is configured to control opening and closing of each first flow passage.
Description
Technical Field
The disclosure belongs to the technical field of microfluidics, and particularly relates to a heating temperature control device and a microfluidic system.
Background
The micro-fluidic chip technology is based on micron-sized fluid control, realizes a complex biochemical reaction process on a small-sized chip, continuously and iteratively upgrades a heavy large-sized analytical instrument in the directions of miniaturization, integration, automation, high flux and the like, and promotes the development of the fields of real-time detection, field analysis and the like which take rapid quantification as a core. However, in the application process of the microfluidic chip, some reactions need to be carried out at a specific temperature, such as gene detection, but the current microfluidic chip cannot realize accurate temperature control.
Disclosure of Invention
The present disclosure is directed to at least one of the problems of the prior art, and provides a heating temperature control device and a micro-fluidic system.
In a first aspect, an embodiment of the present disclosure provides a heating temperature control device, where the heating temperature control device includes: the device comprises a first substrate, a second substrate and a runner cavity chamber layer, wherein the first substrate and the second substrate are oppositely arranged, and the runner cavity chamber layer is positioned between the first substrate and the second substrate;
the first substrate includes: the first heating elements are positioned on one side of the substrate, which is close to the runner cavity layer; the runner cavity layer includes: a plurality of first liquid storage tanks, a plurality of second liquid storage tanks, a plurality of first flow channels and a plurality of second flow channels; the second substrate includes: a main body portion; the main body part is provided with a plurality of through holes, a placing groove and a plurality of switch elements;
an orthographic projection of one of the first reservoirs on the substrate at least partially overlaps an orthographic projection of one of the first heating elements on the substrate; the first flow channel has a first end and a second end, the first end of one of the first flow channels is communicated with one of the first fluid reservoirs, and the second end is communicated with the second fluid reservoir; the second flow channel has a first end and a second end, the first end of one of the second flow channels is communicated with one of the first liquid storage tanks, and the second end is communicated with one of the through holes; the orthographic projection of the placing groove on the substrate is at least partially overlapped with the orthographic projection of the second liquid storage tank on the substrate; the switching element is configured to control opening and closing of each of the first flow passages.
Optionally, the first substrate further includes: a second heating element located on a side of the substrate adjacent to the runner cavity layer;
an orthographic projection of the second reservoir on the substrate at least partially overlaps an orthographic projection of the second heating element on the substrate.
Optionally, the switching element comprises: a gas control layer and a thin film layer which are arranged in a laminated manner; the thin film layer is positioned on one side of the gas control layer close to the runner cavity layer;
the through hole penetrates through the gas control layer and the thin film layer; the placement groove penetrates the gas control layer and the thin film layer.
Optionally, the gas control layer comprises: the gas inlet and the gas outlet are arranged at two ends of the gas flow channel and are deviated from one side of the thin film layer; wherein an extending direction of one of the gas flow passages intersects an extending direction of one of the first flow passages.
Optionally, the second liquid storage tank is disposed in a central region of the runner cavity layer, and the plurality of first liquid storage tanks are uniformly arranged on two sides of the second liquid storage tank along the same direction.
Optionally, the runner chamber layer further comprises: a third flow passage;
the third flow channel has a first end and a second end, the first end of the third flow channel is communicated with the second ends of the plurality of first flow channels, and the second end is communicated with the second liquid storage tank.
Optionally, the first heating element and the second heating element are disposed in the same layer and are made of the same material.
Optionally, the first heating element comprises: one or more of a titanium metal layer and a platinum metal layer.
In a second aspect, an embodiment of the present disclosure provides a microfluidic system, including the heating temperature control device and the microfluidic chip provided above;
the micro-fluidic chip is positioned in the placing groove of the second substrate.
Optionally, the microfluidic chip comprises: polymerase chain reaction chip.
Drawings
Fig. 1 is a schematic structural diagram of a heating temperature control device according to an embodiment of the present disclosure.
Detailed Description
For a better understanding of the technical aspects of the present disclosure, reference is made to the following detailed description taken in conjunction with the accompanying drawings.
Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this disclosure belongs. The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather is used to distinguish one element from another. Also, the use of the terms "a," "an," or "the" and similar referents do not denote a limitation of quantity, but rather denote the presence of at least one. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.
At present, a variety of biochemical reactions can be integrated on a microfluidic chip, and Polymerase Chain Reaction (PCR) is one of the biochemical reactions, is a classic molecular biology experimental technology for synthesizing a large amount of target DNA fragments in vitro through enzymatic Reaction, has the characteristics of strong specificity, high sensitivity, simple and convenient operation and the like, is not only applied to the basic research fields of gene cloning, sequence analysis and the like, but also has wide application in the medical fields of disease diagnosis, pathogen detection and the like. Hereinafter, PCR will be described as an example, and it is understood that the disclosed embodiments are equally applicable to reactions other than PCR. The realization of PCR usually needs to control the temperature of the reaction system, so that the sample sequentially passes through three stages of high-temperature denaturation (95 ℃), low-temperature annealing (55-60 ℃) and constant-temperature extension (72 ℃) and is circulated in one period, and finally the purpose of rapid and large-scale amplification of the target DNA fragment is achieved. There are two main methods for realizing the program temperature control process of the PCR system on the microfluidic chip: one is that the temperature control method is simple and direct, but has high requirements on the processing technology of the heating element, some of the heating elements also need large-area heat dissipation blocks, the cost is high, and the temperature control uniformity can not be ensured generally; secondly, different temperature areas are arranged on the chip, and PCR reaction liquid passes through the temperature areas in sequence through various fluid control technologies, so that the aim of controlling the temperature of the reaction liquid is indirectly fulfilled. In order to solve at least one of the above technical problems, an embodiment of the present disclosure provides a heating temperature control device and a microfluidic system, and the heating temperature control device and the microfluidic system provided in the embodiment of the present disclosure are further described in detail with reference to the accompanying drawings and the detailed description.
Fig. 1 is a schematic structural diagram of a heating temperature control device according to an embodiment of the present disclosure, and as shown in fig. 1, the heating temperature control device includes: the device comprises a first substrate, a second substrate and a runner cavity layer, wherein the first substrate and the second substrate are oppositely arranged, and the runner cavity layer is positioned between the first substrate and the second substrate; the first substrate includes: the device comprises a substrate 101 and a plurality of first heating elements 102 positioned on one side of the substrate 101 close to a runner cavity layer; the runner cavity layer includes: a plurality of first reservoirs 301, second reservoirs 302, a plurality of first flow channels 303, and a plurality of second flow channels 304; the second substrate includes: a main body portion; the main body portion is provided with a plurality of through holes 201, a placement groove 202, and a plurality of switching elements 203; an orthographic projection of a first reservoir 301 onto the substrate 101 at least partially overlaps an orthographic projection of a first heating element 102 onto the substrate 101; the first flow channel 303 has a first end and a second end, the first end of one first flow channel 303 is communicated with one first liquid storage tank 301, and the second end is communicated with the second liquid storage tank 302; the second flow channel 304 has a first end and a second end, the first end of one second flow channel 304 is communicated with one first reservoir 301, and the second end is communicated with one through hole 201; the orthographic projection of the placement groove 202 on the substrate 101 and the orthographic projection of the second liquid reservoir 302 on the substrate 101 at least partially overlap; the switching element 203 is configured to control the opening and closing of each first flow channel 303.
It should be noted that, taking the example that the heating and temperature control device provided in the embodiment of the present disclosure can perform heating and temperature control on a PCR microfluidic chip, the realization of PCR generally needs to perform temperature control on a reaction system, so that a sample sequentially passes through three stages of high temperature denaturation (95 ℃), low temperature annealing (55-60 ℃) and constant temperature extension (72 ℃). Specifically, in the heating and temperature controlling device provided by the embodiment of the present disclosure, the number of the first liquid storage pools 301 is 6, which are respectively labeled as nos. 1 to 6, the number of the second liquid storage pools 302 is 1, correspondingly, the number of the first flow channels 303 is 6, the number of the second flow channels 304 is 6, the number of the first heating elements 102 is 6, the number of the through holes is 6, the number of the placing grooves is 1, and the switching element 203 can control the opening and closing of the 6 first flow channels 303. The heating temperatures of the first heating elements 101 corresponding to each two are 95 ℃, 55-60 ℃ and 72 ℃, respectively, that is, the heating temperatures of the first heating elements 102 corresponding to the first liquid storage tanks 301 No. 1 and No. 6 are 95 ℃, the heating temperatures of the first heating elements 102 corresponding to the first liquid storage tanks 301 No. 2 and No. 5 are 55-60 ℃, and the heating temperatures of the first heating elements 102 corresponding to the first liquid storage tanks 301 No. 3 and No. 6 are 72 ℃.
Before the PCR experiment, reaction liquid is loaded on the PCR microfluidic chip, then the PCR microfluidic chip is placed in the placing groove 202, and then temperature control liquid is introduced into the three first liquid storage pools 301 in different temperature environments through the through holes 201 and the communicated second flow channels 304, namely the temperature control liquid is introduced into the first liquid storage pools 301 No. 1, No. 2 and No. 3. All the first flow paths 303 communicating with the first reservoirs 301 are closed by the switching elements 203, and the temperature-controlled liquid in each of the first reservoirs 301 is heated to 95 ℃, 55 ℃ and 72 ℃. When the reaction starts, the first flow channel 303 communicating the first liquid storage pools 301 No. 1 and No. 6 is opened, the temperature control liquid at 95 ℃ is pushed into the second liquid storage pool 304 through an external injection pump, and then the first flow channel 303 communicating the first liquid storage pools 301 No. 1 and No. 6 is closed, so that the temperature control liquid can heat the PCR microfluidic chip. After the heating is completed, the first channel 303 communicating with the first reservoirs 301 No. 1 and No. 6 is opened, the temperature control liquid is introduced into the first reservoir 304 No. 6 for standby, and then the first channel 303 communicating with the first reservoirs 301 No. 1 and No. 6 is closed. In the next stage, a first flow channel 303 communicated with the No. 2 and No. 5 first liquid storage tanks 301 is opened, the temperature-controlled liquid with the temperature of 55 ℃ is pushed into a second liquid storage tank 304 through an externally connected syringe pump, and other steps are completely the same as the previous steps; and finally, controlling the first flow channel 303 communicated with the first liquid storage pools 301 No. 3 and No. 4 by the same operation, and heating the PCR microfluidic chip by using 72 ℃ temperature control liquid to finish a temperature control period, wherein the temperature control liquid is positioned in the first liquid storage pools No. 6, No. 5 and No. 4. And in the next temperature control period, the temperature control operation is carried out according to the same steps, the liquid returns to the first liquid storage tanks 301 of No. 1, No. 2 and No. 3 from the first liquid storage tanks of No. 6, No. 5 and No. 4, and the like, and about 35-40 circulating temperature control periods are finally completed.
The first heating element 102 of each first liquid storage pool 301 bottom in every temperature control cycle of the heating temperature control device that this disclosed embodiment provided can be used for maintaining the temperature of temperature-controlled liquid in the cavity, avoid temperature-controlled liquid to appear violent temperature fluctuation, can utilize the homogeneity of temperature-controlled liquid self temperature simultaneously, heat the temperature control to PCR micro-fluidic chip, the inhomogeneous problem of temperature that adopts heating element direct heating to the reaction liquid direct heating in PCR micro-fluidic chip to bring has been avoided, avoid directly controlling the reaction liquid loss that PCR reaction liquid brought simultaneously, thereby can promote PCR reaction efficiency, practice thrift the experimental cost.
In some embodiments, as shown in fig. 1, the first substrate further comprises: a second heating element 103 located on a side of the substrate 101 adjacent to the runner cavity layer; the orthographic projection of the second reservoir 302 on the substrate 101 at least partially overlaps the orthographic projection of the second heating element 103 on the substrate 101.
It should be noted that the substrate 101 may be a glass substrate, the glass substrate may be packaged and bonded with the runner cavity layer by means of ultraviolet curing glue, and the second heating element 103 may be disposed on the glass substrate. When the temperature control liquid in the second reservoir 302 is used to heat the PCR microfluidic chip, for example, in a high temperature denaturation stage (95 ℃), the temperature of the temperature control liquid gradually decreases to less than 95 ℃ as the temperature of the temperature control liquid is conducted into the PCR microfluidic chip during the heating process. The temperature of the temperature control liquid in the second liquid storage tank 302 can be collected in real time, if the temperature is lower than 95 ℃, the temperature control liquid is heated by the second heating element 103, the temperature of the temperature control liquid in the cavity is maintained, severe temperature fluctuation of the temperature control liquid is avoided, the temperature uniformity of the temperature control liquid can be utilized, the temperature of the PCR microfluidic chip is controlled by heating, the problem of uneven temperature caused by direct heating of the reaction liquid in the PCR microfluidic chip by the heating element is avoided, meanwhile, the loss of the reaction liquid caused by direct control of the PCR reaction liquid is avoided, the PCR reaction efficiency can be improved, and the experiment cost is saved.
In some embodiments, as shown in fig. 1, the switching element 203 comprises: a gas control layer 2031 and a thin film layer 2032 which are stacked; the thin film layer 2032 is positioned on one side of the gas control layer 2031 near the runner cavity layer; the through-hole 201 penetrates the gas control layer 2031 and the thin film layer 2032; the placement grooves 202 penetrate the gas control layer 2031 and the thin film layer 2032.
It should be noted that the gas control layer 2031 and the thin film layer 2032 may be made of film forming materials such as Polydimethylsiloxane (PDMS), and the two may be packaged and bonded by means of uv curable adhesive, so as to form the switch element 203 having the diaphragm valve structure. The vias 201 may extend through the gas control layer 2031 and the thin film layer 2032 to provide a passage for the temperature control liquid to be introduced into the first reservoir 301. The placement grooves 202 may penetrate the gas control layer 2031 and the thin film layer 2032 to place the PCR microfluidic chip. In practice, the diameter of the through-holes 201 may be 1mm, the overall size of the gas control layer 2031 may be 300mm × 200mm × 5mm, and the overall size of the thin film layer 2032 may be 300mm × 200mm × 1 mm.
In some embodiments, as shown in fig. 1, the gas control layer 2031 comprises: a plurality of gas flow channels, and gas inlets and gas outlets disposed at both ends of the gas flow channels and on a side facing away from the thin film layer 2031; wherein the extending direction of one gas flow channel intersects with the extending direction of one first flow channel 303.
It should be noted that, in practical applications, the cross-sectional dimension of the gas flow channel may be 2mm × 1mm, and the extending direction of the gas flow channel intersects with the extending direction of the first flow channel 303 of the flow channel chamber layer, and preferably, the extending directions of the two may be perpendicular. When the external air enters the air flow channel of the air inlet, the air pressure presses the film layer 2032 below to cause the protrusion thereof to block the first flow channel 303 of the lower layer, so that the opening and closing of the first flow channel 303 can be controlled by controlling the air pressure, thereby controlling the flow path of the temperature control liquid.
In some embodiments, as shown in fig. 1, the second reservoir 302 is disposed in the central region of the chamber layer, and the plurality of first reservoirs 301 are uniformly arranged on both sides of the second reservoir 302 along the same direction.
It should be noted that, in practical applications, the first liquid storage tank 301 and the second liquid storage tank 302 may be uniformly disposed, which is beneficial to the preparation of each liquid storage tank and the corresponding and communicated first flow channel 303 and second flow channel 304, thereby simplifying the preparation process and saving the preparation cost.
In some embodiments, as shown in fig. 1, the runner cavity layer further comprises: a third flow channel 305; the third flow channel 305 has a first end and a second end, the first end of the third flow channel 305 communicates with the second ends of the plurality of first flow channels 303, and the second end communicates with the second reservoir 302.
It should be noted that the third flow channel 305 may be communicated with a plurality of first flow channels 303, and the temperature-controlled liquid in the plurality of first flow channels 303 may be introduced into the second liquid storage tank 304 through the same third flow channel 305, so that the length of the first flow channel 303 may be reduced, thereby simplifying the manufacturing process and saving the manufacturing cost.
In some embodiments, the first heating element 102 and the second heating element 103 are disposed in the same layer and are the same material.
It should be noted that the first heating element 102 and the second heating element 103 are disposed on the same layer and made of the same material, and during the manufacturing process, the two heating elements may be manufactured by the same process, for example, they may be formed by a deposition process using a metal or semiconductor material, so as to simplify the manufacturing process and save the manufacturing cost.
In some embodiments, the first heating element 102 comprises: one or more of a titanium metal layer and a platinum metal layer.
It should be noted that, in practical applications, the first heating element 102 may be made of a single-layer structure, or may be made of a multi-layer structure, so as to improve the heat conduction performance of the first heating element 102. For example, the first heating element 102 includes: one or more of a titanium metal layer and a platinum metal layer. It is understood that the second heating element 102 may also be made with the same structure as the first heating element 102.
In a second aspect, an embodiment of the present disclosure provides a microfluidic system, where the microfluidic system includes the heating device and the microfluidic chip provided in any of the above embodiments, and the microfluidic chip is located in the placement groove of the second substrate.
In the microfluidic system provided by the embodiment of the present disclosure, the first heating element at the bottom of each first liquid storage tank in each temperature control period of the heating temperature control device can be used to maintain the temperature of the temperature control liquid in the chamber, avoid the temperature control liquid to have violent temperature fluctuation, and simultaneously can utilize the uniformity of the temperature control liquid itself, heat the microfluidic chip for temperature control, avoid the problem of uneven temperature caused by the direct heating of the reaction liquid in the microfluidic chip by the heating element, and avoid the loss of the reaction liquid caused by the direct control of the reaction liquid, thereby improving the reaction efficiency and saving the experiment cost.
In some embodiments, a microfluidic chip comprises: polymerase chain reaction chip.
It should be noted that the microfluidic chip in the microfluidic system provided in the embodiment of the present disclosure may specifically be a Polymerase Chain Reaction (PCR) chip, and in practical applications, the size of the PCR chip may be 50mm × 40mm × 0.5mm, and the central heating area is 25mm × 25 mm. It is understood that the microfluidic chip may also include other microfluidic chips requiring heating reaction, and the implementation principle of the microfluidic chip is the same as that of the PCR chip described above, and is not listed here.
It is to be understood that the above embodiments are merely exemplary embodiments that are employed to illustrate the principles of the present disclosure, and that the present disclosure is not limited thereto. It will be apparent to those skilled in the art that various changes and modifications can be made therein without departing from the spirit and scope of the disclosure, and these are to be considered as the scope of the disclosure.
Claims (10)
1. A heating temperature control device, characterized in that, heating temperature control device includes: the device comprises a first substrate, a second substrate and a runner cavity chamber layer, wherein the first substrate and the second substrate are oppositely arranged, and the runner cavity chamber layer is positioned between the first substrate and the second substrate;
the first substrate includes: the first heating elements are positioned on one side of the substrate, which is close to the runner cavity layer; the runner cavity layer includes: a plurality of first liquid storage tanks, a plurality of second liquid storage tanks, a plurality of first flow channels and a plurality of second flow channels; the second substrate includes: a main body part; the main body part is provided with a plurality of through holes, a placing groove and a plurality of switch elements;
an orthographic projection of one of the first reservoirs on the substrate at least partially overlaps an orthographic projection of one of the first heating elements on the substrate; the first flow channel has a first end and a second end, the first end of one of the first flow channels is communicated with one of the first fluid reservoirs, and the second end is communicated with the second fluid reservoir; the second flow channel is provided with a first end and a second end, the first end of one second flow channel is communicated with one first liquid storage tank, and the second end is communicated with one through hole; the orthographic projection of the placing groove on the substrate is at least partially overlapped with the orthographic projection of the second liquid storage tank on the substrate; the switching element is configured to control opening and closing of each of the first flow passages.
2. The heated temperature control device of claim 1, wherein the first substrate further comprises: a second heating element located on a side of the substrate adjacent to the runner cavity layer;
an orthographic projection of the second reservoir on the substrate at least partially overlaps an orthographic projection of the second heating element on the substrate.
3. The heated temperature control device of claim 1, wherein the switching element comprises: a gas control layer and a thin film layer which are arranged in a laminated manner; the thin film layer is positioned on one side of the gas control layer close to the runner cavity layer;
the through hole penetrates through the gas control layer and the thin film layer; the placement groove penetrates the gas control layer and the thin film layer.
4. The heated temperature control device of claim 3, wherein the gas control layer comprises: the gas inlet and the gas outlet are arranged at two ends of the gas flow channel and are deviated from one side of the thin film layer; wherein an extending direction of one of the gas flow passages intersects an extending direction of one of the first flow passages.
5. The heating temperature control device according to claim 1, wherein the second liquid storage tank is disposed in a central region of the runner cavity layer, and the plurality of first liquid storage tanks are uniformly arranged on both sides of the second liquid storage tank along a same direction.
6. The heating and temperature control device of claim 1, wherein the runner cavity layer further comprises: a third flow path;
the third flow channel has a first end and a second end, the first end of the third flow channel is communicated with the second ends of the plurality of first flow channels, and the second end is communicated with the second liquid storage tank.
7. The heating temperature control device of claim 2, wherein the first heating element and the second heating element are disposed in the same layer and are made of the same material.
8. The heated temperature control device of claim 7, wherein the first heating element comprises: one or more of a titanium metal layer and a platinum metal layer.
9. A microfluidic system comprising the heating temperature control device of any one of claims 1-8 and a microfluidic chip;
the microfluidic chip is positioned in the placing groove of the second substrate.
10. The microfluidic system of claim 9, wherein the microfluidic chip comprises: polymerase chain reaction chip.
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| CN114854570A (en) * | 2022-07-05 | 2022-08-05 | 鲲鹏基因（北京）科技有限责任公司 | Temperature control device, liquid cooling temperature control system and PCR instrument |
| CN114854570B (en) * | 2022-07-05 | 2022-09-02 | 鲲鹏基因（北京）科技有限责任公司 | Temperature control device, liquid cooling temperature control system and PCR instrument |
| WO2024087602A1 (en) * | 2022-10-25 | 2024-05-02 | 广州达安基因股份有限公司 | Isothermal amplification detection device |
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