CN114617133A - Disinfectant and preparation method thereof - Google Patents
Disinfectant and preparation method thereof Download PDFInfo
- Publication number
- CN114617133A CN114617133A CN202111061592.4A CN202111061592A CN114617133A CN 114617133 A CN114617133 A CN 114617133A CN 202111061592 A CN202111061592 A CN 202111061592A CN 114617133 A CN114617133 A CN 114617133A
- Authority
- CN
- China
- Prior art keywords
- sodium hypochlorite
- disinfectant
- carbon dioxide
- hypochlorite solution
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/04—Carbon disulfide; Carbon monoxide; Carbon dioxide
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/20—Elemental chlorine; Inorganic compounds releasing chlorine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Epidemiology (AREA)
- Molecular Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of disinfection, and particularly relates to a disinfectant and a preparation method thereof. The pH value of the disinfectant is 6-6.5, and the preparation raw materials of the disinfectant comprise a sodium hypochlorite solution, water and carbon dioxide. In the system, the applicant selects specific purified water, carbon dioxide and a sodium hypochlorite solution as raw materials, firstly limits the proportion of the carbon dioxide and the sodium hypochlorite, then enables the carbon dioxide to fully react with the sodium hypochlorite by controlling the flow of the carbon dioxide and the sodium hypochlorite, and simultaneously controls the pressure and the temperature of the reaction, so that the sodium hypochlorite is not decomposed, the carbon dioxide does not generate an acidification reaction when fully reacting, the stability of the system is maintained, and the sterilization effect is improved.
Description
Technical Field
The invention relates to the technical field of disinfection, and concretely relates to a disinfectant with an IPC part classification number of A01N59/00 and a preparation method thereof.
Background
The transmission path of infectious diseases, particularly viruses, is mainly through air and contact, and the transmission path is also through contacting the viruses staying on the surface of an object. It is therefore necessary to disinfect the object, skin, air.
Sodium hypochlorite (NaClO) is an inorganic hypochlorite, is a byproduct of chlor-alkali industry, has simple production process, low price and strong bactericidal capacity, and is mainly used as a disinfectant, a bleaching agent and a purifying agent, most of the disinfectant in the market has the defects of instability and poor corrosivity of the sodium hypochlorite disinfectant, in order to ensure that the sodium hypochlorite can be stored for a long time, a certain amount of stabilizer is added when the disinfectant for sodium hypochlorite is produced in patent CN112120042A, so that the reduction of effective chlorine in a sodium hypochlorite solution is reduced, but the stabilizers are usually some organic stabilizers which have certain harm to human bodies, so that the disinfectant is not suitable for human skins, and the organic stabilizers mainly depend on the increase of the pH value of a system to ensure that the system is in an alkaline state to maintain stability, hypochlorite in sodium hypochlorite is easy to react with the organic matters, so that the activity of the system is reduced, and the disinfection effect of the system is reduced.
Disclosure of Invention
In order to solve the technical problem, a first aspect of the present invention provides a disinfection solution, wherein the pH of the disinfection solution is 5.5-6.5, and the raw materials for preparing the disinfection solution include a sodium hypochlorite solution, water and carbon dioxide.
In one embodiment, the disinfecting solution has a pH of 5.5 to 6.3; preferably 6.24.
In one embodiment, the sodium hypochlorite solution has a mass concentration of 5% or more.
In one embodiment, the sodium hypochlorite solution is composed of sodium hypochlorite (CAS number: 7681-52-9) and water, and is food grade.
In a preferred embodiment, the mass concentration of the sodium hypochlorite solution is greater than or equal to 8%; more preferably, the mass concentration of the sodium hypochlorite solution is 10%.
In one embodiment, the sodium hypochlorite solution accounts for 0.1-0.2% of the total volume of the disinfectant raw material; preferably 0.15-0.18%; further preferably 0.17%.
In one embodiment, the volume ratio of the sodium hypochlorite solution to the carbon dioxide is (5-20): 1; preferably (8-12): 1; more preferably 10: 1.
in one embodiment, the water is purified water filtered through a high purity filter and has a purity of 99.9 wt%.
In one embodiment, the carbon dioxide has a CAS number of 124-38-9 and a purity of 99.9 wt%.
Some related researches use raw materials such as acidic electrolyzed water, sodium hypochlorite and the like to prepare a sodium hypochlorite disinfectant, the initial sterilization effect of the prepared disinfectant is mainly because the acidic electrolyzed water originally has a certain sterilization effect, but when the acidic electrolyzed water acts with a sodium hypochlorite solution, the system is in an acidic condition, the corrosivity of the system is increased, and the disinfectant is not suitable for human skin and other parts, the applicant unexpectedly finds that the sterilization performance and the corrosion resistance of the disinfectant can be better by using purified water, the sodium hypochlorite solution and carbon dioxide in the system, and the applicant finds through a large number of experiments that the pH value of the system can be close to neutrality and the corrosivity is reduced by strictly controlling the amounts of the purified water, the sodium hypochlorite solution and the carbon dioxide when the purified water, the sodium hypochlorite solution and the carbon dioxide are in a specific range, particularly, stainless steel is basically not corrosive, and the system has good sterilization effect, probably because specific contents of the three components in the system of the purified water of the system generate certain reaction, the reaction reaches a steady state balance, the pH value and the sterilization activity of the system are maintained, and when sodium hypochlorite or carbon dioxide is increased, the balance of the system is broken, so that the pH value of the system is too high or too low, and the sterilization activity is reduced.
The second aspect of the present invention provides a method for preparing a disinfection solution, comprising the following steps:
(1) adding sodium hypochlorite solution, water and carbon dioxide into different storage tanks according to corresponding volume parts;
(2) firstly, conveying water into a mixing tank;
(3) after the water is conveyed, simultaneously inputting sodium hypochlorite solution and carbon dioxide into the mixing tank;
(4) after sodium hypochlorite solution and carbon dioxide are input, carrying out mixing reaction in a mixing tank to obtain mixed solution;
(5) filtering the mixed solution to obtain the disinfectant.
In one embodiment, the flow rate of the sodium hypochlorite solution in step (3) is 0.6-1.2L/h, preferably 0.8-1L/h, and more preferably 0.9L/h.
In one embodiment, the ratio of the flow rate of carbon dioxide to the flow rate of sodium hypochlorite solution in step (3) is 1: (12-15), preferably 1: 12.5.
in the prior art, carbon dioxide and sodium hypochlorite solution are used as raw materials to prepare the disinfectant, and the addition of carbon dioxide increases the stability of the system to a certain extent, but the sterilization effect is reduced when the effective chlorine content of the disinfectant is influenced to a certain extent due to improper control of the preparation process. Through a great deal of research, the applicant finds that on the basis of selecting purified water by the system, the carbon dioxide and the sodium hypochlorite can be well dissolved and reacted in the purified water by selecting the specific flow of the carbon dioxide and the specific flow of the sodium hypochlorite solution, so that the effective chlorine content is increased, and the prepared disinfectant has good bactericidal property. When the ratio of the flow rate of the carbon dioxide to the flow rate of the sodium hypochlorite solution is increased, the carbon dioxide may not react with the sodium hypochlorite solution in time to cause acidification of the system, and when the ratio of the flow rate of the carbon dioxide to the flow rate of the sodium hypochlorite solution is decreased, the concentration of the system may be increased, and the density and viscosity of the system may be increased, so that the carbon dioxide cannot be well dissolved in the system.
In one embodiment, the temperature of the mixing tank in step (4) is 5 to 10 ℃, preferably 6 to 8 ℃, and more preferably 7 ℃.
In one embodiment, the reaction pressure of the mixing tank in the step (4) is 0.6-1MPa, and the reaction time is 5-10 min; preferably, the reaction pressure of the mixing tank in the step (4) is 0.65-0.85MPa, and the reaction time is 6-9 min; more preferably, in the step (4), the reaction pressure of the mixing tank is 0.8MPa, and the reaction time is 7 min.
In a preferred embodiment, the filtration in step (5) is mucosal grade filtration.
The mucosal grade filtration in the present invention is suitably an RO membrane.
The stability and the bactericidal performance of the disinfectant are characteristics for evaluating the performance of the disinfectant, but the stability and the bactericidal performance of the existing disinfectant are difficult to achieve a better level, in the system of the invention, the applicant firstly researches and selects specific raw materials, then carries out a large number of process tests, finds in the tests that the raw materials can react well by selecting specific reaction pressure, time and temperature aiming at the system, and unexpectedly finds in the tests that the stability and the bactericidal performance of the disinfectant can achieve a better level by combining a mucous membrane grade filtration mode in the last step of preparing the disinfectant, probably because sodium hypochlorite can not be decomposed basically at a lower temperature and a specific pressure, and carbon dioxide can not cause acidification phenomenon of the system basically, and sodium bicarbonate can be decomposed into sodium carbonate to a certain extent in the reaction process, the presence of sodium carbonate and sodium bicarbonate in the system maintains the stability of the reaction to some extent, probably because the concentration of the aqueous solution increases during the reaction, while Na+Is less affected by concentration, and Na+The interaction with water molecules is strong, the system is stable under the constraint action of salt ions, and byproducts are not easy to generate, so that the quality of the disinfectant is high.
In one embodiment, the disinfecting solution can be applied to the air, the surface of an object, or the skin.
In one embodiment, the disinfecting liquid can be applied to metal surfaces, particularly stainless steel surfaces.
In addition, the starting materials used are all commercially available, unless otherwise specified.
Has the advantages that:
1. in the system, the applicant selects specific purified water, carbon dioxide and sodium hypochlorite solution as raw materials, firstly limits the proportion of the carbon dioxide and the sodium hypochlorite, then controls the flow of the carbon dioxide and the sodium hypochlorite to ensure that the carbon dioxide can fully react with the sodium hypochlorite, and simultaneously controls the pressure and the temperature of the reaction to ensure that the sodium hypochlorite is not decomposed, the carbon dioxide does not generate an acidification reaction when fully reacting, the stability of the system is maintained, and the sterilization effect is improved;
2. the disinfectant prepared by the invention has good effect of killing the new coronavirus;
3. the disinfectant disclosed by the invention has good effective rate content and stability, and can also have a good sterilization effect when the stability is ensured;
4. the bactericide of the invention has no corrosivity to stainless steel substrate;
5. the disinfectant liquid port of the present invention is used in air, skin, oral cavity, etc.
Detailed Description
Example 1
A disinfectant is prepared from raw materials including a sodium hypochlorite solution, water and carbon dioxide;
the sodium hypochlorite solution consists of sodium hypochlorite (CAS number: 7681-52-9) and water, and is food grade; the mass concentration of the sodium hypochlorite solution is 10%;
the water is purified water obtained by filtering through a high-purity filter, and the purity of the water is 99.9 wt%;
the CAS number of the carbon dioxide is 124-38-9, and the purity of the carbon dioxide is 99.9 wt%;
the sodium hypochlorite solution accounts for 0.17 percent of the total volume of the raw materials of the disinfectant;
the volume ratio of the sodium hypochlorite solution to the carbon dioxide is 10: 1;
the disinfectant comprises the following preparation steps:
(1) adding sodium hypochlorite solution, water and carbon dioxide into different storage tanks according to corresponding volume parts; (2) firstly, conveying water into a mixing tank; (3) after the water is conveyed, simultaneously conveying sodium hypochlorite solution and carbon dioxide into the mixing tank; (4) after the sodium hypochlorite solution and the carbon dioxide are input, carrying out mixing reaction in a mixing tank to obtain a mixed solution; (5) filtering the mixed solution to obtain a disinfectant;
the flow rate of the sodium hypochlorite solution in the step (3) is 0.9L/h; the ratio of the flow rate of the carbon dioxide to the flow rate of the sodium hypochlorite solution in the step (3) is 1: 12.5;
the temperature of the mixing tank in the step (4) is 7 ℃; in the step (4), the reaction pressure of the mixing tank is 0.8MPa, and the reaction time is 7 min;
the filtration in the step (5) is mucosa-level filtration.
Example 2
The specific implementation mode of the disinfectant is the same as that in example 1, except that the volume ratio of the sodium hypochlorite solution to the carbon dioxide is 3: 1.
example 3
The specific implementation mode of the disinfectant is the same as that in example 1, except that the volume ratio of the sodium hypochlorite solution to the carbon dioxide is 1: 1.
example 4
The specific implementation mode of the disinfectant is the same as that in example 1, except that the volume ratio of the sodium hypochlorite solution to the carbon dioxide is 25: 1.
example 5
The specific implementation manner of the disinfection solution is the same as that in example 1, except that the ratio of the flow rate of the carbon dioxide solution to the flow rate of the sodium hypochlorite solution in the step (3) is 1: 9.
example 6
A disinfection solution, which is substantially the same as that described in example 1, except that the reaction pressure in the mixing tank in step (4) is 0.35 MP.
Example 7
The specific implementation mode of the disinfectant is the same as that in example 1, except that filter paper with the pore size of 2 microns is used for replacing mucosal grade filtration.
Performance testing
1. And (3) corrosion test:
testing the metal sheet: stainless steel, carbon steel, aluminum (circular, diameter 24.0mm, 1.0mm in thickness, and perforated with a 2.0mm diameter orifice having a total surface area of about 9.8017cm2And 4-6 of finish.
The test method comprises the following steps: and soaking different test metal sheets for 74 hours by using the prepared disinfectant with the same amount, and observing the corrosion condition.
And (3) testing environment: the temperature was 25 ℃ and the relative humidity was 46%.
The test results are shown in table 1:
TABLE 1
2. Bactericidal test
2.1 quantitative killing test of bacterial propagules
Test strains: staphylococcus aureus (ATCC 6538) cultured to passage 7; pseudomonad aeruginosa (ATCC 15442) cultured to passage 7; e.coli 8099, cultured to passage 8.
And (3) testing a sample: the disinfectant of example 1.
Neutralizing agent: D/E neutralizes the broth.
Culture medium: nutrient agar culture medium, pressure steam sterilization for standby.
Organic interferents: and (4) neutralizing agent TSB.
Diluent agent: PBS buffer.
Carrier: cloth carrier (specification: 10mm × 10mm, material: plain weave face, degreased), and pressure steam sterilizing.
The detection basis is as follows: the technical Specification for Disinfection (2002 edition) 2.1.1.2-2.1.1.7.
And (3) testing environment: the temperature is 23-25 ℃, and the relative humidity is 50-55%.
And (3) test results: (1) under the test condition, the neutralizing agent of the D/E neutralizing broth is repeated three times, and the inhibiting effect of the disinfectant in the example 1 on bacterial propagules can be effectively neutralized in the test of soaking the carrier and quantitatively killing; the neutralizing agent and the disinfectant thereof have no influence on the growth and the culture of the tested bacteria (staphylococcus aureus); (2) under the test condition, the reaction is repeated for 3 times, the disinfectant in the example 1 acts for 1.0min, and the average killing logarithm value of the disinfectant on staphylococcus aureus, pseudomonas aeruginosa and escherichia coli is not less than 3.00 (the sterilization rate is not less than 99.99%).
2.2 quantitative Candida albicans killing test
Test strains: candida albicans (ATCC 10231) cultured up to passage 5.
And (3) testing a sample: the disinfectant of example 1.
Neutralizing agent: D/E neutralizes the broth.
Culture medium: nutrient agar culture medium, pressure steam sterilization for standby.
Organic interferents: and (4) neutralizing agent TSB.
Diluent agent: PBS buffer.
Carrier: cloth carrier (specification: 10mm × 10mm, material: plain-woven face, degreased), and pressure steam sterilizing.
The detection basis is as follows: the technical Specification for Disinfection (2002 edition) 2.1.1.2-2.1.1.7.
And (3) testing environment: the temperature is 23-25 ℃, and the relative humidity is 50-55%.
And (3) test results: (1) under the test condition, the neutralizing agent of the D/E neutralizing broth is repeated three times, and the inhibiting effect of the disinfectant in the example 1 on bacterial propagules can be effectively neutralized in the test of soaking the carrier and quantitatively killing; the neutralizing agent and the disinfectant thereof have no influence on the growth and the culture of the tested bacteria (candida albicans); (2) the reaction of the disinfectant in example 1 was repeated 3 times under the test conditions, and the action of the disinfectant in example 1 was 1.0min, and the bactericidal rate of Candida albicans was ≧ 3.00 (99.99%).
2.3. Killing microorganism test
The killing effect on Acinetobacter baumannii in example 1 was tested according to specification for disinfection (2002) 2.2.1.2.1, 2.1.1.5.5 and 2.1.1.7.4.
The test results obtained: after 3 times of rereading tests, under the condition of a constant temperature test at 20 ℃, the disinfectant in the embodiment 1 acts on 10minhou, and the killing log values of the acinetobacter baumannii are all more than 5.00.
3. Simulation and use test testing
3.1 hand Disinfection in situ test
A disinfectant: the disinfectant of example 1.
Neutralizing agent: D/E neutralized broth.
Sterile cotton swab: the disposable medical cotton swab is sterilized for later use.
Culture medium: TSA (tryptone soy agar medium), pressure steam sterilization for use.
A subject: x30 workers.
Diluent agent: PBS buffer containing 0.1% Tween-80.
The detection basis is as follows: disinfection Specification (2002 edition) 2.1.2.6 Positive control group, negative control group and test group samples were prepared according to the method of Disinfection Specification (2002 edition) 2.1.2.10, and 1.0ml of positive control group, test group and negative control group samples were taken at appropriate dilutions, respectively, inoculated into a plate by agar pour-on method, incubated at 37 deg.C for 48h, and the results were observed as shown in Table 2
TABLE 2
3.2 object surface Sterilization site simulation
A disinfectant: the disinfectant of example 1.
Neutralizing agent: D/E neutralizes the broth.
Sterile cotton swab: the disposable medical cotton swab is sterilized for later use.
Culture medium: TSA (tryptone soy agar medium), pressure steam sterilization for use.
A subject: xx company office table, door.
Diluent agent: PBS buffer containing 0.1% Tween-80.
The detection basis is as follows: the positive control group, negative control group and test group samples were prepared according to the method of "Disinfection Specification" (2002 edition) 2.1.2.10, and then 1.0ml of each sample was aspirated, plates were inoculated by the agar pour method, 2 plates were inoculated per sample, incubated in a 37 ℃ incubator for 48 hours, and the final results were observed as shown in Table 3:
TABLE 3
3.3 on-site simulation of air sanitization
Disinfectant: the disinfectant of example 1.
Neutralizing agent: D/E neutralizes the broth.
Test strains: staphylococcus albus 8032, passage 5, and bacterial suspension formulated with 0.03 mlo/LPBS.
Sampling liquid: nutrient broth medium (containing olive oil and neutralizing agent).
The spray contamination device: an aerosol atomizer.
Air microorganism sampling device: six-level sieve mesh air impact type sampler.
Culture medium: nutrient broth culture medium, nutrient agar culture medium, pressure steam sterilizing for use,
diluent (b): PBS buffer.
The detection basis is as follows: the tests of contamination, sampling and sterilization were carried out according to the method of Disinfection Specification (2002 edition) 2.1.3.4, and the test was repeated 3 times.
And (3) test environment: the temperature is 22-25 ℃, and the relative humidity is 50-55%.
And (3) test results: the disinfectant in the embodiment 1 is acted for 30min at the temperature of 22-25 ℃ and the relative humidity of 50-55%. The killing rate of the artificially polluted staphylococcus albus in a closed room with 20 square meters is 99.95 percent, 99.95 percent and 99.96 percent respectively after 3 times of test results.
3.4 field test of air Disinfection Effect
A disinfectant: the disinfectant of example 1.
Neutralizing agent: D/E neutralizes the broth.
Test strains: air natural bacteria.
Sampling liquid: nutrient broth medium (containing olive oil and neutralizing agent).
Air microorganism sampling device: six-level sieve mesh air impact sampler.
Culture medium: nutrient broth culture medium, nutrient agar culture medium, pressure steam sterilizing for use,
the detection basis is as follows: the sampling and sterilization tests were carried out according to the method of Disinfection Specification (2002 edition) 2.1.3.5, and the test was repeated 3 times.
And (3) test environment: the temperature is 22-25 ℃, and the relative humidity is 50-55%.
And (3) test results: the disinfectant in the embodiment 1 is acted for 30min at the temperature of 22-25 ℃ and the relative humidity of 50-55%. The results of 3 times of tests on the natural bacteria in 20 square meters of closed room air are 96.96%, 97.13% and 96.09% respectively.
4. Available chlorine content test
Test samples: the disinfectant of example 1.
The test basis is as follows: the test was carried out according to the Disinfection Specification 2.2.1.2.1 (2002 edition).
And (3) testing environment: the temperature was 25 ℃ and the relative humidity was 47%.
And (3) testing results: 152 mg/L.
5. Contaminant content testing
Test samples: the disinfectant of example 1.
The test basis is as follows: chapter IV (1.2-1.4) of technical Specification for cosmetic safety (2015 edition) of the national food and drug administration.
And (3) testing environment: the temperature was 25 ℃ and the relative humidity was 46%.
And (3) testing results: the lead content is less than 0.05 mg/L; the arsenic content is less than 0.01 mg/L; the mercury content is less than 0.005 mg/L.
6. Virus inactivation assay
Test materials: disinfectant solutions of example 1, Vero E6 cells, SARS-CoV-2, DMEM medium (Gibco), FBS (Gibco), diabody (Gibco), 96-well cell medium, pancreatin (TPCK treated), CO2 cell incubator, biosafety cabinet, and the like.
And (3) experimental design: the assay was divided into 6 groups, groups 1-3: in the FBS control group, after the virus (0.1mL) and the PBS (0.9mL) are respectively incubated at room temperature for 0.5min, 1.5min and 3min, Vero E6 cells are immediately diluted by 10 times, and the virus titer is calculated after 74 h; groups 4-6: in the disinfectant solution group of example 1, after the virus (0.1mL) and the disinfectant solution (0.9mL) of example 1 were incubated at room temperature for 0.5min, 1.5min and 3min, respectively, Vero E6 cells were inoculated by 10-fold dilution immediately, and the virus titer was calculated after 74 hours.
The test steps are as follows: (1) vero E6 cells in good growth status were split into 96-well cell culture plates in P2 laboratories at 37 ℃ with 5% CO2Culturing in an incubator, and transferring to a P3 laboratory test through a transfer window when the early cell confluence degree of the next day reaches about 70%; (2) in a biological safety cabinet, taking 0.9mL of the disinfectant in the embodiment 1 to a sterile centrifuge tube, then adding 0.1mL of SARS-CoV-2 virus, acting in a greenhouse for 0.5min, 1.5min and 3min, and immediately using, and carrying out 10-fold gradient dilution on a DMEM culture medium (containing double antibody and 2% FBS); the control group adopts sterile FBS, 0.9mLFBS is taken into a sterile centrifuge tube, then 0.1mL of SARS-CoV-2 virus is added, the greenhouse effect is carried out for 0.5min, 1.5min and 3min, then the control group is immediately used, and DMEM culture medium (containing double antibody and 2% FBS) is subjected to 10 times of gradient dilution; (3) discarding the culture medium in 96-well plate, gently patting dry on sterile absorbent paper, adding the dilution obtained in step (2) to 96-well Vero E6 cell plate with 8 repeated wells per gradient at 37 ℃ and 5% CO2Observing cytopathic condition after culturing for 3d in an incubator, calculating probability and TCID of virus50(ReD-muench's method); (4) for rechecking cytopathic effect observed by naked eyes, the supernatant was pooled in duplicate wells of the same gradient, and 140ml of the supernatant was subjected to viral RNA extraction (140ml of the supernatant +5.6ul of carrier RNA, mixed well, allowed to act at room temperature for 10 min) according to the instructions of the viral RNA extraction kit (QIAGEN), 500ul of absolute ethanol was added and mixed well, and the surface of the tube was finished with 75% ethanolCarrying out the subsequent steps after the whole sterilization and taking out of a P3 laboratory); (5) performing fluorescence quantitative qPCR amplification on the RNA extracted in the step (4) by adopting a TaqMan probe method, detecting the Ct value of the ORFlab gene, and objectively judging whether the cell is infected; (6) considering that the virus treated by the disinfectant in the step (2) may have cytotoxicity, cause cell death and influence judgment result when added into cells at low dilution, the untreated virus is diluted by 10 gradients in DMEM (double antibody-containing PBS-containing 2%) and added into empty 96-well plates without cells, each gradient has 8 repeated wells, and the temperature is 37 ℃, and 5% CO2And (3) taking out the culture box after placing for 3 days, combining 8 repeated wells, then carrying out fluorescence quantitative qPCR amplification by using a TaqMan probe method, detecting the Ct value (considering that the Ct value is in a standard Ct value curve) of the ORFlab gene in the virus liquid after different gradient dilutions without being amplified by the Vero E6 cells, comparing the Ct value with the Ct value in the step (5), and determining whether obvious replication is carried out after the virus subjected to different treatments and different gradient dilutions in the step (2) is inoculated into Vero E6 cells (note: if the virus is replicated, the Ct value is reduced). The test results are shown in table 4:
TABLE 4
7. Stability test
The disinfectant solution of example 1 was kept at a temperature of 54 ℃ for 14 days according to the specification for disinfection (2002 edition) 2.2.1.2.1, and the results were observed. The reduction rate was calculated and the results are shown in Table 5.
Test of pH value
Test samples:
and (3) testing environment: the temperature was 22 ℃ and the relative humidity was 46%.
The pH was measured using a PHS-25pH meter. The results are shown in Table 5:
TABLE 5
| pH value | Effective rate content decrease rate (%) | |
| Example 1 | 6.24 | 9.2 |
| Example 2 | 4.23 | 10.6 |
| Example 3 | 3.25 | 10.8 |
| Example 4 | 7.92 | 11.2 |
| Example 5 | 4.56 | 13.6 |
| Example 6 | 4.14 | 17.6 |
| Example 7 | 6.96 | 12.63 |
The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as can be conceived and the embodiments presented herein are merely illustrative of selected implementations in accordance with a combination of all possible embodiments. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Where numerical ranges are used in the claims, also included are sub-ranges within them, and variations in these ranges are also to be construed as being covered by the appended claims where possible.
Claims (10)
1. The disinfectant is characterized in that the pH value of the disinfectant is 5.5-6.5, and the raw materials for preparing the disinfectant comprise a sodium hypochlorite solution, water and carbon dioxide.
2. The disinfecting solution of claim 1, wherein the concentration of the sodium hypochlorite solution is greater than 5% by mass.
3. The disinfectant liquid as set forth in claim 1 or 2, wherein the sodium hypochlorite solution is 0.1-0.2% of the total volume of the disinfectant liquid raw materials.
4. The disinfecting solution of claim 1, wherein the volume ratio of the sodium hypochlorite solution to the carbon dioxide is (5-20): 1.
5. a method for preparing a disinfecting liquid according to any one of claims 1-4, characterized by comprising the following steps:
(1) adding sodium hypochlorite solution, water and carbon dioxide into different storage tanks according to corresponding volume parts;
(2) firstly, conveying water into a mixing tank;
(3) after the water is conveyed, sodium hypochlorite solution and carbon dioxide are input into the mixing tank;
(4) after the sodium hypochlorite solution and the carbon dioxide are input, carrying out mixing reaction in a mixing tank to obtain a mixed solution;
(5) filtering the mixed solution to obtain the disinfectant.
6. The method of claim 5, wherein the flow rate of the sodium hypochlorite solution in step (3) is 0.6-1.2L/h.
7. The method as claimed in claim 5 or 6, wherein the ratio of the flow rate of carbon dioxide to the flow rate of sodium hypochlorite solution in step (3) is 1: (12-15).
8. The method of claim 5, wherein the temperature of the mixing tank in step (4) is 5-10 ℃.
9. The method of claim 5 or 8, wherein the mixing tank in step (4) has a reaction pressure of 0.6-1MPa and a reaction time of 5-10 min.
10. The method for preparing disinfecting solution as claimed in claim 5, wherein the filtration in step (5) is mucosa-grade filtration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111061592.4A CN114617133A (en) | 2021-09-10 | 2021-09-10 | Disinfectant and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111061592.4A CN114617133A (en) | 2021-09-10 | 2021-09-10 | Disinfectant and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114617133A true CN114617133A (en) | 2022-06-14 |
Family
ID=81896677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111061592.4A Pending CN114617133A (en) | 2021-09-10 | 2021-09-10 | Disinfectant and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114617133A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007031374A (en) * | 2005-07-28 | 2007-02-08 | Sutakku System:Kk | Method for producing germicidal disinfectant solution |
| CN103027073A (en) * | 2013-01-05 | 2013-04-10 | 马惠祥 | Generating device for weak hypochlorous acid disinfectant with no need for power supply |
| CN206940428U (en) * | 2017-06-22 | 2018-01-30 | 山东威巴克消毒技术有限公司 | Hypochlorous acid thimerosal process units with spraying mechanism |
| CN111109288A (en) * | 2020-03-04 | 2020-05-08 | 盛毅 | Preparation method and device of efficient environment-friendly disinfectant |
| CN111346532A (en) * | 2020-03-13 | 2020-06-30 | 唐山市新概念科技有限公司 | Preparation method of carbonic acid-hypochlorous acid water |
-
2021
- 2021-09-10 CN CN202111061592.4A patent/CN114617133A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007031374A (en) * | 2005-07-28 | 2007-02-08 | Sutakku System:Kk | Method for producing germicidal disinfectant solution |
| CN103027073A (en) * | 2013-01-05 | 2013-04-10 | 马惠祥 | Generating device for weak hypochlorous acid disinfectant with no need for power supply |
| CN206940428U (en) * | 2017-06-22 | 2018-01-30 | 山东威巴克消毒技术有限公司 | Hypochlorous acid thimerosal process units with spraying mechanism |
| CN111109288A (en) * | 2020-03-04 | 2020-05-08 | 盛毅 | Preparation method and device of efficient environment-friendly disinfectant |
| CN111346532A (en) * | 2020-03-13 | 2020-06-30 | 唐山市新概念科技有限公司 | Preparation method of carbonic acid-hypochlorous acid water |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Larson et al. | Inactivation of Bacillus subtilis spores with ozone and monochloramine | |
| Liltved et al. | Inactivation of bacterial and viral fish pathogens by ozonation or UV irradiation in water of different salinity | |
| Gluhchev et al. | Electrochemically activited water: biophysical and biological effects of anolyte and catholyte types of water | |
| Ignatov et al. | Preparation of electrochemically activated water solutions (catholyte/anolyte) and studying their physical-chemical properties | |
| WO2005117914A2 (en) | Biocidal solution | |
| Ignatov et al. | Studying the antimicrobial and antiviral effects of electrochemically activated NaCl solutions of anolyte and catholyte on a strain of e. coli dh5 and classical swine fever (csf) virus | |
| Dow et al. | The impact of selected water quality parameters on the inactivation of Bacillus subtilis spores by monochloramine and ozone | |
| KR20180054631A (en) | Hypochloric acid aqueous solution | |
| US20160113282A1 (en) | Virus disinfectant containing chlorous acid aqueous solution | |
| Gluhchev et al. | Studying the virucidal and biocidal effects of electrochemically activited anolyte and catholyte types of water on classical swine fever virus (CSF) and bactrerium E. coli DH5 | |
| Ahn et al. | Effects of extended storage of chlorhexidine gluconate and benzalkonium chloride solutions on the viability of Burkholderia cenocepacia | |
| Zhang et al. | The aggregation of Aspergillus spores and the impact on their inactivation by chlorine-based disinfectants | |
| CN111629593A (en) | Antimicrobial agent comprising hypochlorous acid | |
| CN114617133A (en) | Disinfectant and preparation method thereof | |
| JP2003144001A (en) | Raising water for marine organism, method and apparatus for producing raising water | |
| Zhou et al. | Effect of pipe material and low level disinfectants on biofilm development in a simulated drinking water distribution system | |
| Taharaguchi et al. | Effect of weak acid hypochlorous solution on selected viruses and bacteria of laboratory rodents | |
| Sasahara et al. | Efficacy and effectiveness of showerheads attached with point-of-use (POU) filter capsules in preventing waterborne diseases in a Japanese hospital | |
| Amajor et al. | Comparative evaluation of the physicochemical composition and microbial quality of seven different water sources used in processing root and tuber crops in Umudike, Nigeria | |
| JP2021079381A (en) | Bacillus dominance apparatus, method for relative evaluation of the amount of bacillus bacteria, and wastewater treatment method using the same | |
| CN114916543B (en) | Disinfection stock solution and preparation method and application thereof | |
| CN115245174B (en) | Water-soluble compound potassium hydrogen persulfate disinfectant and preparation method thereof | |
| Yang et al. | Study on an air filter material immobilized with bio-antimicrobials | |
| CN114916543A (en) | Disinfection stock solution and preparation method and application thereof | |
| Saprykina et al. | The decontamination effect of UV radiation with respect to micromycetes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |