CN114609020B

CN114609020B - Flow cytometer

Info

Publication number: CN114609020B
Application number: CN202210122550.5A
Authority: CN
Inventors: 谷为岳; 谷为陵; 华源
Original assignee: Chineo Medical Technology Co ltd
Current assignee: Chineo Medical Technology Co ltd
Priority date: 2022-02-09
Filing date: 2022-02-09
Publication date: 2022-10-25
Anticipated expiration: 2042-02-09
Also published as: CN114609020A

Abstract

The utility model relates to a flow cytometer relates to medical instrument's technical field, and it includes organism and solution tank, the vertical baffle that is provided with in the solution tank, and will sheath liquid pool and waste liquid pool are separated into to the solution tank, the lower extreme level of baffle is provided with the filtration pore, be provided with the pellicle in the filtration pore. This application realizes the filtration of waste liquid through setting up the pellicle, makes the limpid sheath liquid in the waste liquid pond flow back to the sheath liquid pond again in, realizes the filtration and the recovery of waste liquid and recycles, the effectual cost that has reduced.

Description

Flow cytometer

Technical Field

The application relates to the field of medical equipment, in particular to a flow cytometer.

Background

Flow cytometers are devices that automatically analyze and sort cells. It can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can select specified cell subsets according to the preselected parameter range.

In order to improve the detection precision of the flow cytometer, the sheath flow principle is usually applied, and the mainly adopted reagent is sheath liquid which is a balanced dielectric solution without fluorescence background, and the main components of the sheath liquid are sodium chloride, potassium chloride, disodium ethylene diamine tetraacetate and a bacteriostatic agent.

In the prior art, a waste liquid generated after a sample is processed by a flow cytometer is a mixture of a sample liquid and a sheath liquid, and the waste liquid is generally processed in a centralized manner after an experiment is finished. Therefore, a large amount of sheath fluid needs to be prepared for experiments in the experiment process, and the cost is high and needs to be improved.

Disclosure of Invention

To reduce costs, the present application provides a flow cytometer.

The flow cytometer provided by the application adopts the following technical scheme:

the utility model provides a flow cytometer, includes organism and solution tank, the vertical baffle that is provided with in the solution tank, and will sheath liquid pond and waste liquid pond are separated into to the solution tank, the lower extreme level of baffle is provided with the filtration pore, be provided with the pellicle in the filtration pore.

Through adopting above-mentioned technical scheme, when the cell appearance during operation, the sheath liquid in the sheath liquid pond is carried to the organism in, and waste liquid discharges to the waste liquid pond simultaneously. Meanwhile, under the effect of the semipermeable membrane, the filtration of the waste liquid is realized, cells and impurities are retained, and clear sheath liquid in the waste liquid pool can flow back to the sheath liquid pool again, so that the waste liquid is timely filtered, the sheath liquid is recycled, and the cost is effectively reduced.

Optionally, the level is provided with and is located respectively in the baffle filter hole top and below unreel the axle and the rolling axle, the both ends of pellicle are convoluteed in unreel the axle with the rolling axle, and be provided with the control on the baffle unreel the axle with the synchronous rotatory actuating mechanism of rolling axle.

Through adopting above-mentioned technical scheme, when filtering the waste liquid, it is rotatory to utilize actuating mechanism control to unreel axle and rolling axle, utilizes the semi-permeable membrane after the rolling axle will use to carry out the rolling, utilizes unreeling the axle simultaneously and unreels new semi-permeable membrane. Realize the timely change of pellicle this moment to guarantee to the stable filtration and the processing of waste liquid, realize the stable recovery of sheath liquid and recycle.

Optionally, the driving mechanism includes a pulley, a belt, a ratchet wheel and a ratchet bar, the pulley is disposed at two ends of the unwinding shaft and the winding shaft, and the belt is wound on a pair of pulleys on the same side; the ratchet wheels are arranged at two ends of the unwinding shaft and the winding shaft, the ratchet bar is vertically and slidably connected to the partition plate, and the tooth keys on the ratchet bar are obliquely and upwards arranged, can only upwards turn and are meshed with the ratchet wheels; the upper ends of the ratchet bars are provided with driving rods, and the outer wall of the upper end of each ratchet bar is sleeved with a spring positioned between the driving rod and the upper end face of the partition plate.

Through adopting above-mentioned technical scheme, when changing the pellicle, press the actuating lever and drive the downward motion of ratchet bar, make the tooth key on the ratchet bar overturn. Then the driving rod is loosened, the ratchet bar is controlled to move upwards under the action of the spring, the ratchet bar is utilized to control the ratchet wheel to rotate at the moment, and the unwinding shaft and the winding shaft are controlled to synchronously rotate under the action of the belt and the belt wheel, so that the semipermeable membrane is quickly replaced. Therefore, the semi-permeable membrane can be timely replaced and quantitatively replaced by the driving mechanism which is simple in structure and convenient to operate.

Optionally, vertical sliding connection has the increased pressure board in the waste liquid pond, the last vertical forcing pipe that is provided with of increased pressure board, it is provided with a plurality of switch holes to run through on the increased pressure board, and goes up the terminal surface horizontal smooth connection and be used for covering the switch board in switch hole.

Through adopting above-mentioned technical scheme, when filtering the waste liquid, close the switch board earlier, then press the increased pressure board downstream, exert pressure to the waste liquid, make the waste liquid pass through the pellicle fast, accelerate the filtration to the waste liquid. Subsequently, the switch board is opened, the pressurizing board is controlled to move upwards, the operation is repeated, the high-efficiency filtration of the waste liquid is realized, and the recovery efficiency of the waste liquid is improved.

Optionally, a control rod is vertically and slidably connected in the pressure tube, an outer edge is arranged on the outer wall of the lower end of the control rod, and an inner edge abutting against the outer edge is arranged on the inner wall of the upper end of the pressure tube; the outer edge is hinged with a plurality of connecting rods, the connecting rods penetrate through the pressure pipe and are hinged to the upper end face of the switch board, and the upper end of the control rod is provided with a linkage rod connected to the driving rod.

Through adopting above-mentioned technical scheme, when pressing actuating lever reciprocating motion to realize the change in-process of pellicle, the actuating lever drives trace and control lever simultaneous movement. At the moment, differential motion is generated between the control rod and the pressurizing pipe firstly to realize the opening and closing control of the switch plate, and then the pressurizing plate is controlled to synchronously move to realize the pressurization and depressurization of the waste liquid pool. Consequently through utilizing the linkage cooperation, when changing the pellicle, the filtration efficiency of waste liquid is accelerated, improves the use convenience of whole device.

Optionally, the outer wall of the pressurizing plate is provided with a ring of sealing ring.

Through adopting above-mentioned technical scheme, through setting up sealing washer increase increased pressure plate leakproofness all around, avoid gas leakage, make simultaneously the pressure plate can stop each position in the waste liquid pond, realize the automatic fixation when the pressure plate does not use.

Optionally, a clamping groove for vertically sliding and embedding the partition is formed in the inner side wall of the solution tank, and a locking rod for compressing the upper end face of the partition is rotatably connected to the upper end of the solution tank.

Through adopting above-mentioned technical scheme, through setting up the baffle of sliding to set up the locking lever of rotation type, realize the quick assembly disassembly of baffle, guarantee the quick maintenance of baffle and change.

Optionally, the both sides of baffle are all vertical to be provided with the sealing strip, the inner wall of draw-in groove is provided with the confession the seal groove of sealing strip embedding.

Through adopting above-mentioned technical scheme, through the cooperation that sets up sealing strip and seal groove, increase the leakproofness between sheath liquid pond and the waste liquid pond, prevent that clean sheath liquid from receiving the pollution.

Optionally, the baffle includes left casing and right casing, the level is provided with a plurality of inserted bars on the casing of a left side, be provided with the confession on the casing of the right side the jack of inserted bar grafting embedding.

Through adopting above-mentioned technical scheme, through setting up the left casing and the right casing into convenient dismouting with the baffle to realize the convenient change of each subassembly in the baffle.

In summary, the present application includes at least one of the following beneficial technical effects:

the filtering of the waste liquid is realized by arranging the semipermeable membrane, so that the clear sheath liquid in the waste liquid tank flows back to the sheath liquid tank again, the filtering and recycling of the waste liquid are realized, and the cost is effectively reduced;

the semipermeable membrane which is convenient to replace is arranged, so that stable filtration and treatment of waste liquid are guaranteed, and stable recycling of sheath liquid is realized;

through utilizing the linkage cooperation, when changing the pellicle, the filtration efficiency of waste liquid is quickened, improves the use convenience of whole device.

Drawings

Fig. 1 is a schematic overall structure diagram of an embodiment of the present application.

FIG. 2 is a schematic view of the internal structure of a solution tank in the example of the present application.

Fig. 3 is a schematic view of the internal structure of the separator in the embodiment of the present application.

FIG. 4 is a schematic view of the structure of a solution tank in the embodiment of the present application.

Description of the reference numerals: 1. a body; 11. a liquid inlet pipe; 12. a liquid outlet pipe; 2. a solution pool; 21. a sheath fluid bath; 22. a waste liquid tank; 23. a card slot; 24. a lock lever; 25. a sealing groove; 3. a partition plate; 31. a filtration pore; 32. a semi-permeable membrane; 33. unwinding the reel; 34. a winding shaft; 35. a sealing strip; 36. a left housing; 37. a right housing; 38. inserting a rod; 39. a jack; 4. a drive mechanism; 41. a pulley; 42. a belt; 43. a ratchet wheel; 44. a ratchet bar; 45. a toothed key; 46. a stopper; 47. a drive rod; 48. a spring; 5. a pressurizing plate; 51. a seal ring; 52. a switch hole; 53. a switch plate; 54. a pressurizing pipe; 55. a control lever; 56. a linkage rod; 57. an outer edge; 58. an inner edge; 59. a connecting rod.

Detailed Description

The present application is described in further detail below with reference to figures 1-4.

The embodiment of the application discloses a flow cytometer.

Referring to fig. 1 and 2, the flow cytometer includes a body 1 and a solution reservoir 2. Solution pond 2 sets up in the dorsal part of organism 1 to vertically in solution pond 2 be provided with baffle 3, baffle 3 separates into sheath liquid pond 21 and waste liquid pond 22 with solution pond 2, sets up the feed liquor pipe 11 and the drain pipe 12 that have intercommunication sheath liquid pond 21 and waste liquid pond 22 respectively on the organism 1.

Referring to fig. 1 and 2, a filtering hole 31 is horizontally formed at the lower end of the partition plate 3, and a semi-permeable membrane 32 is disposed in the filtering hole 31 to block cells and impurities in the waste liquid tank 22 and to limit only the passage of clean sheath liquid, so as to filter the waste liquid in the waste liquid tank 22 and recycle the clean sheath liquid.

When the cytometer is in operation, the sheath liquid in the sheath liquid tank 21 is transported into the machine body 1 through the liquid inlet pipe 11, and meanwhile, the waste liquid is discharged into the waste liquid tank 22 through the liquid outlet pipe 12. Meanwhile, under the action of the semipermeable membrane 32, the automatic filtration of the waste liquid can be realized, and the clear sheath liquid in the waste liquid tank 22 can flow back to the sheath liquid tank 21 again, so that the recycling of the sheath liquid is realized.

Referring to fig. 2 and 3, an unwinding shaft 33 and a winding shaft 34 are horizontally disposed in the partition plate 3, and the unwinding shaft 33 and the winding shaft 34 are respectively located above and below the filtering holes 31. Wherein the semi-permeable membrane 32 is wound on the unwinding shaft 33, passes through the filtering hole 31 and then is wound on the winding shaft 34.

Referring to fig. 2 and 3, the separator 3 is provided with a driving mechanism 4 for controlling the unwinding shaft 33 and the winding shaft 34 to rotate synchronously, so as to realize the replacement of the semipermeable membrane 32 during the experiment. The drive mechanism 4 includes a pulley 41, a belt 42, a ratchet wheel 43, and a ratchet bar 44.

Referring to fig. 2 and 3, the pulleys 41 are provided at both ends of the unwinding shaft 33 and the winding shaft 34, and are located inside the separator 3. The belt 42 is wound around a pair of pulleys 41 on the same side for controlling the unwinding shaft 33 and the winding shaft 34 to rotate synchronously.

Referring to fig. 2 and 3, the ratchet 43 is provided at both ends of the unwinding shaft 33 and the winding shaft 34, and the ratchet 43 is provided inside the pulley 41. The ratchet bar 44 is vertically slidably connected to the partition board 3, and the upper end thereof penetrates the upper end surface of the partition board 3.

Referring to fig. 2 and 3, the rack gear 45 of the ratchet bar 44 is disposed obliquely upward and is rotatably connected to the ratchet bar 44, and the ratchet bar 44 is provided with a stopper 46 abutting against the rack gear 45 to limit the rack gear 45 from being flipped upward only and engaged with the ratchet wheel 43, and to control the ratchet wheel 43 to rotate when the ratchet bar 44 moves upward.

Referring to fig. 2 and 3, a driving rod 47 is horizontally disposed between the upper ends of the pair of ratchet bars 44 located outside the barrier for controlling the synchronous movement of the pair of ratchet bars 44. The outer wall of the upper end of each ratchet bar 44 is sleeved with a spring 48, and the spring 48 is positioned between the driving rod 47 and the upper end face of the partition board 3, so that the automatic reset control of the driving rod 47 is realized.

After the waste liquid is filtered for a period of time, the driving rod 47 is pressed to drive the ratchet bar 44 to move downwards, at this time, the driving rod 47 presses the spring 48 to compress, and simultaneously, the ratchet wheel 43 presses the toothed key 45 on the ratchet bar 44 to turn upwards. Until the ratchet bar 44 moves by one unit, the driving rod 47 is released and the ratchet bar 44 is controlled to move upward automatically under the action of the spring 48.

Meanwhile, the toothed key 45 on the ratchet bar 44 engages with the ratchet wheel 43 and controls the ratchet wheel 43 to rotate, and simultaneously controls the unwinding shaft 33 and the winding shaft 34 to synchronously rotate under the action of the belt 42 and the pulley 41. Utilize the semi-permeable membrane 32 after the rolling axle 34 will use afterwards to roll, utilize simultaneously to unreel the axle 33 with new semi-permeable membrane 32, realize semi-permeable membrane 32's quick replacement and ration change, make new semi-permeable membrane 32 be in filtration pore 31 to guarantee to the stable filtration and the processing of waste liquid.

Referring to fig. 2 and 3, a pressurizing plate 5 is vertically and slidably connected to the waste liquid tank 22, and a sealing ring 51 abutting against the inner wall of the waste liquid tank 22 is disposed around the pressurizing plate 5 to apply pressure to the waste liquid in the waste liquid tank 22, so as to accelerate the filtering efficiency of the waste liquid.

Referring to fig. 2 and 3, a plurality of switch holes 52 are formed through the pressure plate 5, the switch holes 52 are distributed at the outer edge of the pressure plate 5, and meanwhile, a switch plate 53 for covering the switch holes 52 is horizontally and movably connected to the upper end surface of the pressure plate 5, so as to realize the on-off control of the switch holes 52, thereby ensuring the stable sliding control of the pressure plate 5.

Referring to fig. 2 and 3, a pressure pipe 54 is vertically arranged on the pressure plate 5, a control rod 55 is vertically and slidably connected in the pressure pipe 54, a linkage rod 56 is horizontally arranged at the upper end of the control rod 55, and the linkage rod 56 is connected to the driving rod 47. The outer wall of the lower end of the control rod 55 is provided with an outer edge 57, and the inner wall of the upper end of the pressurizing pipe 54 is provided with an inner edge 58 which is abutted against the upper end face of the outer edge 57 to limit the sliding distance of the control rod 55.

Referring to fig. 2 and 3, a plurality of connecting rods 59 are hinged to the outer edge of the outer rim 57, and the connecting rods 59 correspond to the positions of the switch plate 53. The connection rod 59 penetrates the pressure pipe 54, and the lower end thereof is hinged to the upper end surface of the opening/closing plate 53, so that the opening/closing plate 53 can be controlled to be opened/closed in the sliding process of the control rod 55.

When the driving rod 47 is pressed to move downwards, the driving rod 47 drives the linkage rod 56 and the control rod 55 to move downwards synchronously. At this time, the control rod 55 slides downwards first, and drives the connecting rod 59 to move synchronously, and then the connecting rod 59 drives the switch plate 53 to gradually cover the switch hole 52. With the further downward movement of the control rod 55, the control rod 55 will drive the pressurizing pipe 54 and the pressurizing plate 5 to synchronously move downward, and apply pressure to the waste liquid tank 22, thereby accelerating the filtration of the waste liquid.

When the driving rod 47 is pressed to move downwards, the control rod 55 moves upwards first, and the switch plate 53 is controlled to be separated from the switch hole 52, until the outer edge 57 of the control rod 55 abuts against the inner edge 58 of the inner wall of the pressurizing pipe 54, the pressurizing pipe 54 and the pressurizing plate 5 are driven to move upwards synchronously, and the reset control of the pressurizing plate 5 is realized. This makes it possible to efficiently filter the waste liquid when the semipermeable membrane 32 is replaced.

Referring to fig. 3 and 4, the inner side walls of the two sides of the solution tank 2 are both vertically provided with a clamping groove 23, and the clamping grooves 23 are used for the two sides of the partition 3 to vertically slide and insert. Meanwhile, the upper end of the solution tank 2 is rotatably connected with a lock rod 24, and the lock rod 24 is used for pressing the upper end surface of the partition plate 3 so as to fix the partition plate 3.

Referring to fig. 3 and 4, sealing strips 35 are vertically arranged on both sides of the partition plate 3, and a sealing groove 25 for embedding the sealing strips 35 is formed in the inner wall of the clamping groove 23, so that the sealing performance between the partition plate 3 and the solution tank 2 is increased, and the stable isolation between the sheath liquid tank 21 and the waste liquid tank 22 is ensured.

Referring to fig. 3 and 4, the partition plate 3 includes a left housing 36 and a right housing 37, and a chamber for accommodating each component is provided on a side where the left housing 36 and the right housing 37 are close to each other. The outer edge of one side of the left shell 36 close to the right shell 37 is horizontally and uniformly provided with a plurality of insertion rods 38, and one side of the right shell 37 close to the left shell 36 is provided with insertion holes 39 for inserting the insertion rods 38.

When each component in the partition board 3 needs to be replaced, the lock rod 24 is firstly rotated to separate the lock rod 24 from the partition board 3, and then the partition board 3 is pulled to move upwards, so that the partition board 3 is taken out and unloaded. And then the left shell 36 and the right shell 37 which form the partition board 3 are controlled to be away from each other, so that the partition board 3 is opened, and at the moment, each component can be replaced.

Finally, the left shell 36 and the right shell 37 are buckled together, so that the partition 3 can be assembled, then the partition 3 is inserted into the clamping groove 23 on the inner wall of the solution tank 2 again, and the sealing strip 35 is embedded into the sealing groove 25. Finally, the locking rod 24 is rotated to press the upper end face of the partition plate 3, so that the partition plate 3 is installed and fixed.

The implementation principle of the flow cytometer in the embodiment of the present application is as follows: when the cytometer is in operation, the sheath fluid in the sheath fluid reservoir 21 is transported into the body 1, and the waste fluid is discharged into the waste fluid reservoir 22. Meanwhile, under the action of the semipermeable membrane 32, the waste liquid is filtered, and the sheath liquid is recycled. After filtering a period to the waste liquid, the pellicle 32 after the control rolling axle 34 will use carries out the rolling, utilizes simultaneously to unreel axle 33 with new pellicle 32, realizes pellicle 32's quick replacement. Meanwhile, when the waste liquid is filtered, the pressure plate 5 is used for applying pressure to the waste liquid, so that the waste liquid can rapidly pass through the semipermeable membrane 32, and the filtering of the waste liquid is accelerated.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: equivalent changes in structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. A flow cytometer, comprising: the device comprises a machine body (1) and a solution tank (2), wherein a partition plate (3) is vertically arranged in the solution tank (2) and divides the solution tank (2) into a sheath liquid tank (21) and a waste liquid tank (22), a filtering hole (31) is horizontally formed in the lower end of the partition plate (3), and a semipermeable membrane (32) is arranged in the filtering hole (31);

an unwinding shaft (33) and a winding shaft (34) which are respectively positioned above and below the filtering hole (31) are horizontally arranged in the partition plate (3), two ends of the semipermeable membrane (32) are wound on the unwinding shaft (33) and the winding shaft (34), and a driving mechanism (4) for controlling the unwinding shaft (33) and the winding shaft (34) to synchronously rotate is arranged on the partition plate (3);

the driving mechanism (4) comprises a belt wheel (41), a belt (42), a ratchet wheel (43) and a ratchet rack (44), the belt wheel (41) is arranged at two ends of the unwinding shaft (33) and the winding shaft (34), and the belt (42) is wound on a pair of the belt wheels (41) on the same side;

the ratchet wheel (43) is arranged at two ends of the unwinding shaft (33) and the winding shaft (34), the ratchet bar (44) is vertically connected to the partition board (3) in a sliding manner, and a toothed key (45) on the ratchet bar (44) is obliquely and upwards arranged, can only be upwards turned and is meshed with the ratchet wheel (43);

a driving rod (47) is arranged at the upper end of each ratchet bar (44), and a spring (48) positioned between the driving rod (47) and the upper end face of the partition plate (3) is sleeved on the outer wall of the upper end of each ratchet bar (44);

a pressurizing plate (5) is vertically connected in the waste liquid pool (22) in a sliding manner, a pressurizing pipe (54) is vertically arranged on the pressurizing plate (5), a plurality of switch holes (52) are arranged on the pressurizing plate (5) in a penetrating manner, and a switch plate (53) used for covering the switch holes (52) is horizontally and movably connected to the upper end surface of the pressurizing plate (5);

a control rod (55) is vertically and slidably connected in the pressurizing pipe (54), the outer wall of the lower end of the control rod (55) is provided with an outer edge (57), and the inner wall of the upper end of the pressurizing pipe (54) is provided with an inner edge (58) abutting against the outer edge (57);

a plurality of connecting rods (59) are hinged to the outer edge (57), the connecting rods (59) penetrate through the pressurizing pipe (54) and are hinged to the upper end face of the switch plate (53), and the upper end of the control rod (55) is provided with a linkage rod (56) connected to the driving rod (47).

2. A flow cytometer as described in claim 1 wherein: the outer wall of the pressurizing plate (5) is provided with a circle of sealing ring (51).

3. A flow cytometer as described in claim 1 wherein: the inner side wall of the solution tank (2) is provided with a clamping groove (23) for the partition board (3) to vertically slide and be embedded, and the upper end of the solution tank (2) is rotatably connected with a locking rod (24) for compressing the upper end surface of the partition board (3).

4. A flow cytometer as described in claim 3, wherein: the both sides of baffle (3) are all vertical to be provided with sealing strip (35), the inner wall of draw-in groove (23) is provided with the confession sealing groove (25) of sealing strip (35) embedding.

5. A flow cytometer as described in claim 4 wherein: baffle (3) are including left casing (36) and right casing (37), left side casing (36) are improved level and are provided with a plurality of inserted bars (38), be provided with the confession on right casing (37) inserted bar (38) jack (39) of pegging graft embedding.