CN114606127A

CN114606127A - Based on biotechnology is with two-way booster-type cell culture equipment

Info

Publication number: CN114606127A
Application number: CN202210327967.5A
Authority: CN
Inventors: 黄达刚
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-03-31
Filing date: 2022-03-31
Publication date: 2022-06-10

Abstract

The invention relates to the technical field of biology, in particular to a bidirectional supercharged cell culture device based on biotechnology. The technical problem is as follows: solid materials are extremely easy to cause accumulation after being broken, so that the solid materials are difficult to be fully mixed with liquid materials, the solid materials are difficult to be fully broken, and the mixed materials are extremely easy to cause accumulation when being heated, so that the heating is insufficient and uneven. The technical scheme is as follows: a two-way booster-type cell culture equipment based on biotechnology comprises a bearing plate, a limiting box and the like; the middle part of the upper surface of the bearing plate is fixedly connected with a limit box. According to the invention, the solid material and the liquid material are premixed, then the solid material is subjected to secondary crushing treatment, then the crushed solid material is mixed with the liquid material, so that the material accumulation is avoided, the later-stage reaction speed of the material is improved, then the mixed material is uniformly dispersed and heated in multiple directions, the material is uniformly and fully heated, and the later-stage culture effect is improved.

Description

Based on biotechnology is with two-way booster-type cell culture equipment

Technical Field

The invention relates to the technical field of biology, in particular to a bidirectional supercharged cell culture device based on biotechnology.

Background

Cell culture refers to a method for simulating an in vivo environment in vitro to enable the cell to survive, grow, reproduce and maintain a main structure and a main function, and is also called as a cell cloning technology, the formal term in biology is a cell culture technology which is an essential process for the whole bioengineering technology, the cell culture technology is an important and common technology in a cell biology research method, a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of the cells and the like of the cells can be researched by the cell culture technology;

therefore, the cell culture device is frequently used in the cell culture process, but in the prior art, the solid material and the liquid material are required to be fully mixed when the cell culture is carried out, in the process, in order to improve the dissolving speed of the two materials, the solid materials are crushed and then mixed in the prior art, and the mode ensures that the solid materials are easy to accumulate after being crushed, thereby causing the accumulated solid materials to be difficult to be fully mixed with the liquid materials, and causing the solid materials to be difficult to be fully crushed by a single crushing process, thereby causing the slow reaction speed of the mixed materials in the later period, simultaneously the mixed materials are not dispersed in time in the heating process, therefore, the accumulation is easy to cause, the heating direction is fixed, the materials are easy to be insufficiently and unevenly heated, and the final culture effect is influenced.

In summary, there is a need to develop a bi-directional pressurizing type cell culture apparatus based on biotechnology to overcome the above problems.

Disclosure of Invention

The invention provides a bidirectional supercharged cell culture device based on biotechnology, which aims to overcome the defects that solid materials are easy to accumulate after being crushed, so that the solid materials are difficult to be fully mixed with liquid materials, the solid materials are difficult to be fully crushed, and the mixed materials are easy to accumulate during heating, so that the mixed materials are not fully and uniformly heated.

The technical scheme is as follows: a two-way booster-type cell culture device based on biotechnology comprises a bearing plate, a limiting box, an incubator, a support frame, a blanking unit, a mixing unit and a heating unit; the middle part of the upper surface of the bearing plate is fixedly connected with a limit box; the upper part of the limiting box is connected with an incubator in a sliding way; the left part of the upper surface of the bearing plate and the right part of the upper surface of the bearing plate are respectively fixedly connected with a supporting frame; a blanking unit for blanking and premixing solid materials and liquid materials is connected between the two support frames; a mixing unit for crushing and mixing the solid material and the liquid material is connected between the two support frames; the mixing unit is connected with the blanking unit; the two support frames are connected with the mixing unit; the lower part of the mixing unit is connected with a heating unit used for dispersing and heating the mixed materials; the heating unit is fixedly connected with the two support frames.

In addition, it is particularly preferable that the blanking unit comprises a fixed frame, a cylinder, a feeding cylinder, a first electric push rod, a sliding plate, a connecting rod, a plug plate, a guide pipe, a sliding block, a round rod, a first spring, a spray head, a motor, a spline shaft, a shaft sleeve, a first straight gear, a first bevel gear, a second straight gear, a second electric push rod and a first fixed plate; the upper surfaces of the two supporting frames are fixedly connected with a fixing frame; a cylinder is fixedly connected between the two fixing frames; the upper part of the cylinder is communicated with two feeding cylinders; the upper parts of the two fixing frames are fixedly connected with a first electric push rod; a sliding plate is fixedly connected between the two first electric push rod telescopic parts; two connecting rods are fixedly connected to the middle part of the sliding plate; the lower parts of the two connecting rods are fixedly connected with a plug plate; the two plug plates are respectively connected with a feeding cylinder in a sliding way; the lower parts of the two feeding cylinders are fixedly connected with a guide pipe; the middle part of the left guide pipe is connected with a sliding block in a sliding way; two round rods are fixedly connected with the middle part of the left guide pipe; the two round rods are connected with the sliding block in a sliding manner; a first spring is sleeved on the outer sides of the two round rods, one end of the first spring is fixedly connected with the guide pipe, and the other end of the first spring is fixedly connected with the sliding block; the middle part of the left guide pipe is communicated with a spray head; the upper part of the right fixed frame is provided with a motor; a spline shaft is fixedly connected with an output shaft of the motor; the spline shaft is rotationally connected with the right fixing frame and the right supporting frame; the middle part of the spline shaft is in transmission connection with a shaft sleeve; the upper part of the shaft sleeve is fixedly connected with a first straight gear; a first bevel gear is fixedly connected to the upper part of the shaft sleeve and below the first straight gear; the lower part of the shaft sleeve is fixedly connected with a second straight gear; the middle part of the right supporting frame is fixedly connected with a second electric push rod; the telescopic part of the second electric push rod is fixedly connected with a first fixing plate; the first fixing plate is rotatably connected with the shaft sleeve; the cylinder and the first spur gear are both connected to the mixing unit.

In addition, it is particularly preferable that the inside of the guide tube is arranged to be large in the upper part and small in the lower part, so that a larger impact force is generated when the material flows out.

Furthermore, it is particularly preferred that the slider is arranged in the shape of an inverted cone for plugging the left catheter.

In addition, it is particularly preferred that the mixing unit comprises a heating cylinder, a first circular ring, a first external toothed ring, a fixed block, an internal toothed ring, a connecting plate, a first connecting shaft, a third spur gear, an impeller, a second fixed plate and a stirring rod; a heating cylinder is fixedly connected between the two support frames; the upper part of the heating cylinder is rotationally connected with a first circular ring; the first ring is rotationally connected with the cylinder; a first outer gear ring is fixedly connected to the outer side of the first circular ring; the first external gear ring is meshed with the first straight gear; four fixed blocks are fixedly connected with the inner side of the first circular ring in an annular and equidistant manner; an inner toothed ring is fixedly connected among the four fixed blocks; the middle part of the inner side of the first circular ring is connected with a connecting plate in a sliding way; the upper part of the connecting plate is rotatably connected with a first connecting shaft; a third straight gear is fixedly connected to the lower part of the first connecting shaft; the middle part of the first connecting shaft is fixedly connected with an impeller; two second fixing plates are fixedly connected to the lower portion of the inner side of the first circular ring, and the heights of the two second fixing plates are different from top to bottom; two opposite sides of the second fixing plates are fixedly connected with a stirring rod; the heating cylinder is connected with the heating unit.

Furthermore, it is particularly preferred that the upper part of the first connecting shaft is provided with a conical projection for crushing the solid material.

In addition, it is particularly preferable that the outer circumferential surface of the stirring rod is provided with a plurality of projections at equal intervals in a ring shape for rolling while stirring the materials.

In addition, it is particularly preferred that the heating unit comprises a second connecting shaft, a sieve plate, a second bevel gear, a guide cylinder, a heating plate, a dispersing cylinder, a collecting cylinder, a second ring, a second outer gear ring, a scraping plate, a push rod, a rolling plate, a ball head and a second spring; the upper part of the heating cylinder is rotationally connected with two second connecting shafts; a sieve plate is fixedly connected between the two second connecting shafts; a second bevel gear is fixedly connected to the right part of the second connecting shaft on the right side; the upper part of the inner side of the heating cylinder is fixedly connected with a guide cylinder; a plurality of heating plates are annularly and equidistantly arranged in the middle of the inner side of the heating cylinder; the lower part of the guide cylinder is fixedly connected with a dispersion cylinder; a collecting cylinder is fixedly connected between the two supporting frames; the upper part of the collecting cylinder is rotatably connected with a second circular ring; the second ring is rotationally connected with the heating cylinder; a second outer gear ring is fixedly connected to the outer side of the second circular ring; a scraper is fixedly connected with the inner side of the second circular ring; a plurality of ejector rods are fixedly connected to the lower surface of the scraper at equal intervals; the lower part of the scraper is connected with a rolling plate in a sliding way; the rolling plate is contacted with the dispersing cylinder; the lower part of the rolling plate is fixedly connected with a ball head; two second springs are fixedly connected between the rolling plate and the scraper.

Furthermore, it is particularly preferred that the dispersing cylinder is provided in the shape of a cone for dispersing the material.

In addition, it is particularly preferred that the lower part of the outer surface of the dispersing cylinder is provided with a plurality of hemispherical lugs at equal intervals in a ring shape, and the hemispherical lugs are used for matching the ball head to move.

The invention has the beneficial effects that: according to the invention, the solid material and the liquid material are premixed, then the solid material is subjected to secondary crushing treatment, then the crushed solid material is mixed with the liquid material, so that the material accumulation is avoided, the later-stage reaction speed of the material is improved, then the mixed material is uniformly dispersed and heated in multiple directions, the material is uniformly and fully heated, and the later-stage culture effect is improved.

Drawings

FIG. 1 shows a schematic diagram of a first three-dimensional configuration of a bi-directional pressurized cell culture apparatus for biotechnology-based according to the present invention;

FIG. 2 shows a second schematic perspective view of the bi-directional pressurizing type cell culture apparatus for biotechnology-based according to the present invention;

FIG. 3 shows a partial cross-sectional view of a bi-directional pressurizing type cell culture apparatus for biotechnology-based according to the present invention;

FIG. 4 shows a schematic three-dimensional structure of a feeding unit of the bi-directional pressurizing type cell culture equipment for biotechnology based on the present invention;

FIG. 5 shows a first partial cross-sectional view of a feeding unit of the bi-directional pressurizing type cell culture apparatus for biotechnology-based of the present invention;

FIG. 6 shows a second partial cross-sectional view of a feeding unit of the bi-directional pressurizing type cell culture equipment for biotechnology-based according to the present invention;

FIG. 7 is a partial perspective view of the blanking unit of the bi-directional pressurizing type cell culture equipment for biotechnology according to the present invention;

FIG. 8 shows a first partial cross-sectional view of a mixing unit of a bi-directional pressurized cell culture apparatus for biotechnology-based use according to the present invention;

FIG. 9 shows a second partial sectional view of a mixing unit of the biotechnological-based two-way plenum cell culture apparatus of the present invention;

FIG. 10 is a partial perspective view of a mixing unit of the biotechnological-based two-way pressure-charged cell culture apparatus according to the present invention;

FIG. 11 is a schematic perspective view of a heating unit of the bi-directional pressurizing type cell culture apparatus for biotechnology-based according to the present invention;

FIG. 12 shows a partial cross-sectional view of a heating unit of the biotechnological-based two-way pressure-charged cell culture apparatus according to the invention;

FIG. 13 is a schematic view showing a first partial perspective configuration of a heating unit of the bi-directional pressurizing type cell culture apparatus for biotechnology based on the present invention;

FIG. 14 shows a second partial perspective view of the heating unit of the bi-directional pressurizing type cell culture equipment for biotechnology according to the present invention.

Description of reference numerals: 1-bearing plate, 2-limit box, 3-incubator, 4-support frame, 201-fixed frame, 202-cylinder, 203-feeding cylinder, 204-first electric push rod, 205-sliding plate, 206-connecting rod, 207-plug plate, 208-conduit, 209-sliding block, 210-round rod, 211-first spring, 212-spray head, 213-motor, 214-spline shaft, 215-shaft sleeve, 216-first straight gear, 217-first bevel gear, 218-second straight gear, 219-second electric push rod, 220-first fixed plate, 301-heating cylinder, 302-first ring, 303-first outer ring gear, 304-fixed block, 305-inner ring gear, 306-connecting plate, 307-first connecting shaft, 308-a third spur gear, 309-an impeller, 310-a second fixing plate, 311-a stirring rod, 401-a second connecting shaft, 402-a sieve plate, 403-a second bevel gear, 404-a guide cylinder, 405-a heating plate, 406-a dispersing cylinder, 407-a collecting cylinder, 408-a second circular ring, 409-a second outer gear ring, 410-a scraping plate, 411-an ejector rod, 412-a rolling plate, 413-a ball head and 414-a second spring.

Detailed Description

The invention is further described with reference to the following figures and detailed description.

Example 1

A two-way booster-type cell culture device based on biotechnology is shown in figures 1-3 and comprises a bearing plate 1, a limiting box 2, an incubator 3, a support frame 4, a blanking unit, a mixing unit and a heating unit; the middle part of the upper surface of the bearing plate 1 is fixedly connected with a limit box 2; the middle part of the limiting box 2 is connected with an incubator 3 in a sliding way; the left part of the upper surface and the right part of the upper surface of the bearing plate 1 are respectively fixedly connected with a support frame 4; a blanking unit is connected between the two support frames 4; a mixing unit is connected between the two support frames 4; the mixing unit is connected with the blanking unit; the two support frames 4 are connected with the mixing unit; the lower part of the mixing unit is connected with a heating unit; the heating unit is fixedly connected with the two support frames 4.

When the device works, the bearing plate 1 is moved to a use position by a worker, the two support frames 4 are kept horizontal, the incubator 3 is placed in the limiting box 2, then solid materials and liquid materials are poured into the discharging unit, then the discharging unit operates to discharge the solid materials and the liquid materials and pre-mix the solid materials and the liquid materials, then the mixed materials flow to the heating unit, the discharging unit operates to drive the mixing unit to operate, at the moment, the mixing unit operates to crush the solid materials and mix the crushed solid materials and the liquid materials, then the discharging unit operates to drive the heating unit to operate after the materials are mixed, at the moment, the heating unit operates to disperse and heat the mixed materials, then the materials flow into the incubator 3, and finally the incubator 3 is taken out by the worker.

Example 2

On the basis of embodiment 1, as shown in fig. 1 and fig. 4 to 14, the blanking unit includes a fixed frame 201, a cylinder 202, a feeding cylinder 203, a first electric push rod 204, a sliding plate 205, a connecting rod 206, a plug plate 207, a guide pipe 208, a slider 209, a round rod 210, a first spring 211, a spray head 212, a motor 213, a spline shaft 214, a shaft sleeve 215, a first straight gear 216, a first bevel gear 217, a second straight gear 218, a second electric push rod 219, and a first fixed plate 220; the upper surfaces of the two support frames 4 are fixedly connected with a fixing frame 201; a cylinder 202 is fixedly connected between the two fixing frames 201; the upper part of the cylinder 202 is communicated with two feeding cylinders 203; the upper parts of the two fixing frames 201 are both connected with a first electric push rod 204 through bolts; a sliding plate 205 is fixedly connected between the telescopic parts of the two first electric push rods 204; two connecting rods 206 are fixedly connected to the middle part of the sliding plate 205; the lower parts of the two connecting rods 206 are fixedly connected with a plug plate 207; two plug plates 207 are each slidably connected to one of the feed cylinders 203; the lower parts of the two feeding cylinders 203 are fixedly connected with a guide pipe 208; the middle part of the left guide pipe 208 is connected with a sliding block 209 in a sliding way; two round rods 210 are fixedly connected to the middle part of the left guide pipe 208; the two round rods 210 are connected with the sliding block 209 in a sliding way; a first spring 211 is sleeved outside each of the two round rods 210, one end of the first spring 211 is fixedly connected to the guide pipe 208, and the other end of the first spring 211 is fixedly connected to the sliding block 209; the middle part of the left guide pipe 208 is communicated with a spray head 212; the upper part of the right fixed frame 201 is provided with a motor 213; a spline shaft 214 is fixedly connected with an output shaft of the motor 213; the spline shaft 214 is rotationally connected with the right fixing frame 201 and the right supporting frame 4; the middle part of the spline shaft 214 is in transmission connection with a shaft sleeve 215; a first straight gear 216 is fixedly connected to the upper part of the shaft sleeve 215; a first bevel gear 217 is fixedly connected to the upper part of the shaft sleeve 215 and below the first straight gear 216; a second spur gear 218 is fixedly connected to the lower part of the shaft sleeve 215; the middle part of the right supporting frame 4 is fixedly connected with a second electric push rod 219; the telescopic part of the second electric push rod 219 is fixedly connected with a first fixing plate 220; the first fixing plate 220 is rotatably connected with the shaft sleeve 215; both the cylinder 202 and the first spur gear 216 are connected to the mixing unit.

The inner side of the conduit 208 is arranged to be big at the top and small at the bottom, so that the material flows out to generate larger impact force.

The slider 209 is arranged in an inverted cone shape for plugging the left catheter 208.

The mixing unit comprises a heating cylinder 301, a first circular ring 302, a first external toothed ring 303, a fixing block 304, an internal toothed ring 305, a connecting plate 306, a first connecting shaft 307, a third spur gear 308, an impeller 309, a second fixing plate 310 and a stirring rod 311; a heating cylinder 301 is fixedly connected between the two support frames 4; the upper part of the heating cylinder 301 is rotatably connected with a first circular ring 302; the first ring 302 is rotatably connected with the cylinder 202; a first outer gear ring 303 is fixedly connected to the outer side of the first circular ring 302; the first external gear ring 303 meshes with the first straight gear 216; four fixed blocks 304 are fixedly connected to the inner side of the first ring 302 in a ring shape at equal intervals; an inner toothed ring 305 is fixedly connected among the four fixed blocks 304; the middle part of the inner side of the first circular ring 302 is slidably connected with a connecting plate 306; the upper part of the connecting plate 306 is rotatably connected with a first connecting shaft 307; a third spur gear 308 is fixedly connected to the lower part of the first connecting shaft 307; the middle part of the first connecting shaft 307 is fixedly connected with an impeller 309; two second fixing plates 310 are fixedly connected to the lower portion of the inner side of the first ring 302, and the heights of the two second fixing plates 310 are different from top to bottom; the opposite sides of the two second fixing plates 310 are fixedly connected with a stirring rod 311; the heating cartridge 301 is connected to a heating unit.

The upper part of the first connecting shaft 307 is provided with a conical lug for crushing solid materials.

The outer annular surface of the stirring rod 311 is provided with a plurality of projections at equal intervals for rolling while stirring the material.

The heating unit comprises a second connecting shaft 401, a sieve plate 402, a second bevel gear 403, a guide cylinder 404, a heating plate 405, a dispersing cylinder 406, a collecting cylinder 407, a second ring 408, a second outer gear ring 409, a scraping plate 410, a push rod 411, a rolling plate 412, a ball 413 and a second spring 414; the upper part of the heating cylinder 301 is rotatably connected with two second connecting shafts 401; a sieve plate 402 is fixedly connected between the two second connecting shafts 401; a second bevel gear 403 is fixedly connected to the right part of the second connecting shaft 401 at the right part; a guide cylinder 404 is fixedly connected to the upper part of the inner side of the heating cylinder 301; a plurality of heating plates 405 are annularly and equidistantly arranged in the middle of the inner side of the heating cylinder 301; the lower part of the guide cylinder 404 is fixedly connected with a dispersion cylinder 406; a collecting cylinder 407 is fixedly connected between the two supporting frames 4; the upper part of the collecting cylinder 407 is rotatably connected with a second circular ring 408; the second ring 408 is rotatably connected with the heating cylinder 301; a second outer gear ring 409 is fixedly connected to the outer side of the second circular ring 408; a scraper 410 is fixedly connected to the inner side of the second ring 408; a plurality of top rods 411 are fixedly connected to the lower surface of the scraping plate 410 at equal intervals; the lower part of the scraping plate 410 is slidably connected with a rolling plate 412; the millboard 412 is in contact with the dispersion drum 406; a ball head 413 is fixedly connected to the lower part of the rolling plate 412; two second springs 414 are fixed between the rolling plate 412 and the scraper 410.

The dispersion barrel 406 is configured in a conical shape for dispersing the material.

A plurality of hemispheroidal bumps are annularly and equidistantly arranged on the lower part of the outer surface of the dispersing cylinder 406 and are used for matching the ball head 413 to move.

When the device works, a worker pours solid materials into the right feeding cylinder 203 and pours liquid materials into the left feeding cylinder 203, then the solid materials and the liquid materials slowly flow out through the two guide pipes 208, at the moment, the slide block 209 is extruded by the gravity of the liquid materials and moves downwards along with the increase of the poured materials, then the left guide pipe 208 is blocked, meanwhile, the slide block 209 moves downwards to extrude the two first springs 211, at the moment, the solid materials and the liquid materials are stopped to be poured into the two feeding cylinders 203, then the two first electric push rods 204 are started to drive the slide plate 205 to move downwards, the slide plate 205 moves downwards to drive the two connecting rods 206 to move downwards, the two connecting rods 206 move to drive the two plug plates 207 to move downwards, at the moment, the two plug plates 207 move downwards along the inner sides of the two feeding cylinders 203, and then the pressure in the two feeding cylinders 203 is increased, at the moment, the liquid material in the left feeding cylinder 203 is sprayed out through the spray head 212, and meanwhile, the solid material in the right feeding cylinder 203 flows out quickly, and the liquid material sprayed out through the spray head 212 is sprayed to the solid material, so that the solid material contains the liquid material, and the solid material and the liquid material are premixed;

meanwhile, when the solid material flows out through the right guide pipe 208, the solid material which flows out rapidly generates impact force and collides with the first connecting shaft 307, the solid material is further crushed through the conical convex block arranged at the upper part of the first connecting shaft 307, then the solid material continuously falls to the impeller 309, at the moment, the motor 213 is started, the output shaft of the motor rotates to drive the spline shaft 214 to rotate, the spline shaft 214 rotates to drive the shaft sleeve 215 to rotate, the shaft sleeve 215 rotates to drive the first straight gear 216, the first bevel gear 217 and the second straight gear 218 to rotate, at the moment, the first straight gear 216 rotates to drive the first outer gear ring 303 to rotate, and the first outer gear ring 303 rotates to drive the first circular ring 302 to rotate; the first circular ring 302 rotates to drive the four fixing blocks 304 to rotate, the four fixing blocks 304 rotate to drive the inner toothed ring 305 to rotate, the inner toothed ring 305 rotates to drive the third spur gear 308 to rotate, the third spur gear 308 rotates to drive the first connecting shaft 307 to rotate, the first connecting shaft 307 rotates to drive the impeller 309 to rotate, then the impeller 309 rotates to crush the solid material again, so that the solid material is crushed for the second time, at the moment, the premixed material falls on the upper surface of the sieve plate 402, meanwhile, the first circular ring 302 rotates to drive the two second fixing plates 310 to rotate, the two second fixing plates 310 rotate to drive the two stirring rods 311 to rotate, and then the crushed solid material is mixed with the liquid material;

then after the solid material and the liquid material are mixed, the second electric push rod 219 is started to drive the first fixing plate 220 to move downwards, the first fixing plate 220 moves to drive the shaft sleeve 215 to move downwards, the shaft sleeve 215 moves to drive the first straight gear 216, the first bevel gear 217 and the second straight gear 218 to move downwards, at this time, the first straight gear 216 moves downwards to separate from the first external gear ring 303, the first bevel gear 217 moves downwards to be meshed with the second bevel gear 403, then the motor 213 is started, the output shaft rotates to drive the spline shaft 214 to rotate, further all the related components are driven to rotate by the same working principle, further the first bevel gear 217 rotates, the first bevel gear 217 rotates to drive the second bevel gear 403 to rotate, the second bevel gear 403 rotates to drive the second connecting shaft 401 at the right side to rotate, the second connecting shaft 401 at the right side rotates to drive the sieve plate 402 to rotate, further the mixed material flows downwards, at this time, the material will flow downward along the inner side of the guiding cylinder 404, and flow to the outer surface of the dispersing cylinder 406 after flowing through the guiding cylinder 404, then the motor 213 is started, the output shaft thereof rotates to drive the spline shaft 214 to rotate reversely, and further drive all the related components to rotate, further drive the first bevel gear 217 to rotate and drive the second bevel gear 403 to rotate reversely, the second bevel gear 403 rotates to drive the second connecting shaft 401 at the right side to rotate, the second connecting shaft 401 rotates to drive the screen plate 402 to rotate reversely, and further drive the screen plate 402 to rotate and reset, then, the second electric push rod 219 is started to drive the first fixing plate 220 to move upward, the first fixing plate 220 moves to drive the shaft sleeve 215 to move upward, and further drive all the related components to move upward, at this time, the first bevel gear 217 moves upward to disengage the second bevel gear 403, the first straight gear 216 moves upward to disengage the first outer gear ring 303, and at the same time, the second straight gear 218 moves upward to engage with the second outer gear ring 409, then the motor 213 is started, the output shaft thereof rotates to drive the spline shaft 214 to rotate, and further drive all the related components to rotate by the same working principle, and further drive the second spur gear 218 to rotate, the second spur gear 218 rotates to drive the second outer gear ring 409 to rotate, the second outer gear ring 409 rotates to drive the second ring 408 to rotate, the second ring 408 rotates to drive the scraper 410 to rotate, the scraper 410 rotates to drive the plurality of ejector pins 411 to rotate along the outer surface of the dispersing cylinder 406, and further stir the mixed material flowing to the outer surface of the dispersing cylinder 406, so that the mixed material is uniformly distributed on the outer surface of the dispersing cylinder 406, and simultaneously the plurality of heating plates 405 are started to operate, thereby uniformly dispersing and heating the mixed material, and simultaneously, as the mixed material remains at the lower edge of the outer surface of the dispersing cylinder 406, the grinding plate 412 is driven to rotate when the scraper 410 rotates, the grinding plate 412 rotates to drive the ball head 413 to rotate, at this moment, the ball head 413 rotates to touch a plurality of hemispherical bumps arranged on the lower portion of the outer surface of the dispersing cylinder 406 at equal intervals, so that the ball head 413 is extruded to move upwards, the ball head 413 moves to drive the rolling plate 412 to move, the rolling plate 412 moves to compress the two second springs 414, when the ball head 413 rotates and is not extruded, the rolling plate 412 moves and resets through the resilience of the two second springs 414, so that the rolling plate 412 moves in a reciprocating manner, so that the material remained on the lower edge of the outer surface of the dispersing cylinder 406 is removed through the reciprocating movement of the rolling plate 412, the heated material flows downwards along the inner side of the collecting cylinder 407 until flowing into the incubator 3, and finally the incubator 3 is taken out by a worker.

It should be understood that this example is only for illustrating the present invention and is not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.

Claims

1. A two-way booster-type cell culture device based on biotechnology comprises a bearing plate (1), a limiting box (2), an incubator (3) and a support frame (4); the middle part of the upper surface of the bearing plate (1) is fixedly connected with a limit box (2); the middle part of the limiting box (2) is connected with an incubator (3) in a sliding way; the left part of the upper surface and the right part of the upper surface of the bearing plate (1) are respectively fixedly connected with a support frame (4); the device is characterized by also comprising a blanking unit, a mixing unit and a heating unit; a blanking unit for blanking and premixing solid materials and liquid materials is connected between the two support frames (4); a mixing unit for crushing and mixing the solid material and the liquid material is connected between the two support frames (4); the mixing unit is connected with the blanking unit; the two support frames (4) are connected with the mixing unit; the lower part of the mixing unit is connected with a heating unit used for dispersing and heating the mixed materials; the heating unit is fixedly connected with the two support frames (4).

2. The bi-directional pressurizing type cell culture equipment based on biotechnology according to claim 1, wherein the feeding unit comprises a fixed frame (201), a cylinder (202), a feeding cylinder (203), a first electric push rod (204), a sliding plate (205), a connecting rod (206), a plug plate (207), a guide pipe (208), a sliding block (209), a round rod (210), a first spring (211), a spray head (212), a motor (213), a spline shaft (214), a shaft sleeve (215), a first straight gear (216), a first bevel gear (217), a second straight gear (218), a second electric push rod (219) and a first fixed plate (220); the upper surfaces of the two supporting frames (4) are fixedly connected with a fixing frame (201); a cylinder (202) is fixedly connected between the two fixing frames (201); the upper part of the cylinder (202) is communicated with two feeding cylinders (203); the upper parts of the two fixing frames (201) are fixedly connected with a first electric push rod (204); a sliding plate (205) is fixedly connected between the telescopic parts of the two first electric push rods (204); two connecting rods (206) are fixedly connected with the middle part of the sliding plate (205); the lower parts of the two connecting rods (206) are fixedly connected with a plug plate (207); the two plug plates (207) are respectively connected with one feeding cylinder (203) in a sliding way; the lower parts of the two feeding cylinders (203) are fixedly connected with a guide pipe (208); the middle part of the left guide pipe (208) is connected with a sliding block (209) in a sliding way; two round rods (210) are fixedly connected with the middle part of the left guide pipe (208); the two round rods (210) are connected with the sliding block (209) in a sliding way; a first spring (211) is sleeved on the outer sides of the two round rods (210), one end of the first spring (211) is fixedly connected with the guide pipe (208), and the other end of the first spring (211) is fixedly connected with the sliding block (209); the middle part of the left guide pipe (208) is communicated with a spray head (212); the upper part of the right fixed frame (201) is provided with a motor (213); a spline shaft (214) is fixedly connected with an output shaft of the motor (213); the spline shaft (214) is rotationally connected with the right fixing frame (201) and the right supporting frame (4); the middle part of the spline shaft (214) is in transmission connection with a shaft sleeve (215); a first straight gear (216) is fixedly connected to the upper part of the shaft sleeve (215); a first bevel gear (217) is fixedly connected to the upper part of the shaft sleeve (215) and is positioned below the first straight gear (216); a second spur gear (218) is fixedly connected to the lower part of the shaft sleeve (215); a second electric push rod (219) is fixedly connected with the middle part of the support frame (4) at the right side; a first fixing plate (220) is fixedly connected with the telescopic part of the second electric push rod (219); the first fixing plate (220) is rotatably connected with the shaft sleeve (215); the cylinder (202) and the first spur gear (216) are both connected to the mixing unit.

3. The biotechnological bi-directional pressure-charged cell culture apparatus according to claim 2 where the inside of the duct (208) is configured to be large in the top and small in the bottom to generate a larger impact force when the material flows out.

4. A biotechnological-based bidirectional pressure-charged cell culture apparatus according to claim 2 characterised in that the slide (209) is arranged as an inverted cone for plugging the left duct (208).

5. The biotechnological bi-directional pressure-charged cell culture apparatus according to claim 4 where the mixing unit includes a heating cylinder (301), a first ring (302), a first outer ring gear (303), a fixed block (304), an inner ring gear (305), a connecting plate (306), a first coupling shaft (307), a third spur gear (308), an impeller (309), a second fixed plate (310), and a stirring rod (311); a heating cylinder (301) is fixedly connected between the two support frames (4); the upper part of the heating cylinder (301) is rotationally connected with a first circular ring (302); the first circular ring (302) is rotationally connected with the cylinder (202); a first outer gear ring (303) is fixedly connected to the outer side of the first circular ring (302); the first external gear ring (303) is meshed with the first straight gear (216); four fixed blocks (304) are fixedly connected with the inner side of the first circular ring (302) in an annular and equidistant manner; an inner toothed ring (305) is fixedly connected among the four fixed blocks (304); the middle part of the inner side of the first circular ring (302) is connected with a connecting plate (306) in a sliding way; the upper part of the connecting plate (306) is rotatably connected with a first connecting shaft (307); a third spur gear (308) is fixedly connected to the lower part of the first connecting shaft (307); the middle part of the first connecting shaft (307) is fixedly connected with an impeller (309); two second fixing plates (310) are fixedly connected to the lower portion of the inner side of the first circular ring (302), and the heights of the two second fixing plates (310) are different from top to bottom; the opposite sides of the two second fixing plates (310) are fixedly connected with a stirring rod (311); the heating cylinder (301) is connected with the heating unit.

6. A bi-directional pressurizing type cell culture equipment based on biotechnology according to claim 5, wherein the upper part of the first connecting shaft (307) is provided with a tapered projection for crushing the solid material.

7. The biotechnological bi-directional pressure-charged cell culture apparatus according to claim 5 where the outer circumferential surface of the stirring rod (311) is provided with a plurality of projections at equal intervals in a circle for rolling while stirring the material.

8. The biotechnological-based two-way forced cell culture apparatus according to claim 7 where the heating unit includes a second connecting shaft (401), a sieve plate (402), a second bevel gear (403), a draft tube (404), a heating plate (405), a dispersion tube (406), a collection tube (407), a second ring (408), a second external gear ring (409), a scraping plate (410), a lift rod (411), a rolling plate (412), a ball head (413), and a second spring (414); the upper part of the heating cylinder (301) is rotatably connected with two second connecting shafts (401); a sieve plate (402) is fixedly connected between the two second connecting shafts (401); a second bevel gear (403) is fixedly connected to the right part of the right second connecting shaft (401); a guide cylinder (404) is fixedly connected to the upper part of the inner side of the heating cylinder (301); a plurality of heating plates (405) are annularly and equidistantly arranged in the middle of the inner side of the heating cylinder (301); the lower part of the guide shell (404) is fixedly connected with a dispersion shell (406); a collecting cylinder (407) is fixedly connected between the two supporting frames (4); the upper part of the collecting cylinder (407) is rotationally connected with a second circular ring (408); the second circular ring (408) is rotationally connected with the heating cylinder (301); a second external gear ring (409) is fixedly connected to the outer side of the second circular ring (408); a scraper (410) is fixedly connected to the inner side of the second ring (408); a plurality of top rods (411) are fixedly connected to the lower surface of the scraper (410) at equal intervals; the lower part of the scraping plate (410) is connected with a rolling plate (412) in a sliding way; the rolling plate (412) is in contact with the dispersing cylinder (406); a ball head (413) is fixedly connected to the lower part of the rolling plate (412); two second springs (414) are fixedly connected between the rolling plate (412) and the scraper (410).

9. A biotechnological-based two-way pressure-charged cell culture apparatus according to claim 8 characterised in that the dispersion barrel (406) is conically shaped for dispersion of material.

10. The biotechnological bi-directional pressure-charged cell culture apparatus according to claim 8 where the dispersion cylinder (406) has a plurality of hemispheric protrusions arranged annularly and equidistantly on the lower part of the outer surface for movement with the ball head (413).