CN114606112A

CN114606112A - Cell scraper

Info

Publication number: CN114606112A
Application number: CN202210284031.9A
Authority: CN
Inventors: 刘岳; 石佳; 周程辉; 吕红; 李倩; 陈宇业; 申婧佳
Original assignee: Fuwai Hospital of CAMS and PUMC
Current assignee: Fuwai Hospital of CAMS and PUMC
Priority date: 2022-03-22
Filing date: 2022-03-22
Publication date: 2022-06-10
Anticipated expiration: 2042-03-22
Also published as: CN114606112B

Abstract

The application provides a cell scraper, this cell scraper includes: the first tool nose structure is provided with a first tool nose used for scraping cells, and the extending direction of the first tool nose is positioned on a first plane; the cutter handle structure is rotationally connected with the first cutter head structure, the first cutter head structure rotates around a first direction relative to the cutter handle structure, and the first direction is perpendicular to the first plane; and the adjusting structure is connected with the cutter handle structure and the first cutter head structure and is used for controlling the first cutter head structure to rotate relative to the cutter handle structure. When the cell scraping device works, the first knife point of the first knife head structure is firstly made to be attached to the bottom wall of a culture dish, then the first knife head structure is controlled to rotate relative to the knife handle structure through controlling the adjusting structure, so that large-area cells on the bottom wall of the culture dish can be scraped, and the working efficiency is effectively improved.

Description

Cell scraper

Technical Field

The application belongs to the technical field of biological experiment tools, and particularly relates to a cell scraper.

Background

At present, when protein is extracted from adherent cells, cell lysate is usually added firstly, after full lysis and digestion, the cells can adhere poorly or even fall off, at the moment, a disposable cell scraper is used for scraping the cells from a culture dish or a pore plate so as to ensure that all the cells are obtained, and a mixture of the lysate and the cells is transferred to a centrifuge tube by a pipettor for subsequent operation so as to obtain the protein through separation. Because the area that current cell scraper once scraped is limited, need the operator to scrape on culture dish or orifice plate bottom surface back and forth many times repeatedly, lead to operating efficiency lower.

Disclosure of Invention

An object of this application embodiment is to provide a cell scraper to solve the technical problem that the cell scraper work efficiency is low that exists among the prior art.

In order to achieve the purpose, the technical scheme adopted by the application is as follows:

provided is a cell scraper including:

the first tool nose structure is provided with a first tool nose used for scraping cells, and the extending direction of the first tool nose is positioned on a first plane;

the cutter handle structure is rotationally connected with the first cutter head structure, the first cutter head structure rotates around a first direction relative to the cutter handle structure, and the first direction is perpendicular to the first plane;

and the adjusting structure is connected with the cutter handle structure and the first cutter head structure and is used for controlling the first cutter head structure to rotate relative to the cutter handle structure.

In one embodiment, further comprising:

the second tool bit structure is provided with a second tool bit used for scraping cells, and the extending direction of the second tool bit is positioned on the first plane;

the second tool bit structure is rotationally connected to the handle structure, the second tool bit structure rotates around a second direction relative to the handle structure, and the second direction is perpendicular to the first plane;

the adjusting structure is connected to the second tool bit structure and used for controlling the second tool bit structure to rotate relative to the tool shank structure.

In one embodiment, the first tool bit structure is disposed on one side of the tool shank structure, the second tool bit structure is disposed on the other side of the tool shank structure away from the first tool bit structure, and the second direction is opposite to the first direction.

In one embodiment, the adjustment structure comprises:

the first gear is fixedly connected with the first cutter head structure, and a rotating shaft of the first gear is collinear with a rotating shaft of the first cutter head structure;

and the control rod is provided with a first side wall, the first side wall forms a first rack, the first rack is meshed with the first gear, and the control rod is connected to the cutter handle structure in a sliding mode.

In one embodiment, the adjustment structure further comprises:

a second gear fixedly attached to the second cutter head structure, an axis of rotation of the second gear and an axis of rotation of the second cutter head structure being collinear;

the control rod is provided with a second side wall, the second side wall and the first side wall are oppositely arranged, a second rack is formed on the second side wall, and the second rack is meshed with the second gear.

In one embodiment, the adjustment structure further comprises:

and one end of the elastic piece is fixedly connected with the cutter handle structure, and the other end opposite to the one end of the elastic piece is fixedly connected with the control rod and used for driving the control rod to move towards the direction far away from the first gear.

In one embodiment, the control rod comprises a main rod and a control block, the main rod is fixedly connected with the control block, and the control block protrudes out of the main rod;

the handle structure is provided with an opening, the opening is communicated with the accommodating cavity, the main rod is located in the accommodating cavity, and the control block part extends out of the accommodating cavity from the opening.

In one embodiment, the first tool bit structure comprises:

the first rotating arm is rotatably connected to the cutter handle structure;

the first blade is provided with a first mounting groove, the first radial arm part is inserted in the first mounting groove, and the first blade is detachably connected to the first radial arm.

In one embodiment, the second bit structure comprises:

the second rotating arm is rotatably connected to the cutter handle structure;

the second blade is provided with a second mounting groove, the second spiral arm part is inserted in the second mounting groove, and the second blade is detachably connected with the second spiral arm.

In one embodiment, further comprising:

a housing box having a housing space formed therein for housing the first and second blades.

The cell scraper that this application provided's beneficial effect lies in: the cell scraper includes: the cell scraping device comprises a first tool bit structure, a tool handle structure and an adjusting structure, wherein the first tool bit structure is provided with a first tool bit which is used for scraping cells, and the extending direction of the first tool bit is positioned on a first plane; the cutter handle structure is rotationally connected with a first cutter head structure, the first cutter head structure rotates around a first direction relative to the cutter handle structure, and the first direction is perpendicular to a first plane; the adjusting structure is connected to the tool handle structure and the first tool head structure and used for controlling the first tool head structure to rotate relative to the tool handle structure. When the cell scraping device works, the first knife point of the first knife head structure is firstly made to be attached to the bottom wall of a culture dish, then the first knife head structure is controlled to rotate relative to the knife handle structure through controlling the adjusting structure, so that large-area cells on the bottom wall of the culture dish can be scraped, and the working efficiency is effectively improved.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.

FIG. 1 is a diagram illustrating an operation state of a cell scraper according to an embodiment of the present disclosure;

FIG. 2 is an exploded view of a cell scraper according to an embodiment of the present application;

FIG. 3 is a partial schematic structural diagram of a cell scraper provided in an embodiment of the present application;

FIG. 4 is a cross-sectional view of a cell scraper provided in an embodiment of the present application;

fig. 5 is a storage state diagram of the cell scraper according to the embodiment of the present application.

Wherein, in the figures, the respective reference numerals:

100. a cell scraper; 110. a first tool bit structure; 111. a first swing arm; 112. a first blade; 120. a knife handle structure; 130. an adjustment structure; 131. a first gear; 132. a control lever; 133. a main rod; 134. a control block; 135. a first rack; 136. a second gear; 137. a second rack; 138. an elastic member; 140. a second bit structure; 141. a second swing arm; 142. a second blade; 150. an accommodating box.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present application clearer, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings that is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present application.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

The cell scraper provided in the examples of the present application will now be described.

As shown in fig. 1, 2, 3, 4, and 5, an embodiment of the present application provides a cell scraper 100, the cell scraper 100 including: first tool tip structure 110, tool shank structure 120, and adjustment structure 130.

The first tip structure 110 has a first tip for scraping cells. Specifically, the first blade tip may be located at the front end of the first blade head structure 110, and the first blade tip may be linear, so as to ensure that the entire blade tip can be attached to the bottom wall of the culture dish. The extending direction of the first tool tip is positioned in a first plane. In particular, the first plane may be a bottom wall of the culture dish, and in case the culture dish is placed on a horizontal plane, the first plane may also be a horizontal plane.

Handle structure 120 is pivotally connected to first handle structure 110. Specifically, an end of the tool shank structure 120 is rotatably connected to an end of the first tool bit structure 110, and the first tool bit structure 110 can rotate relative to the tool shank structure 120. The direction of rotation of the first tool tip structure 110 relative to the tool shank structure 120 may be a first direction, and the first direction is perpendicular to the first plane. Under the condition that the first plane is a horizontal plane, the first direction is a vertical direction, that is, the first tool tip structure 110 rotates on the horizontal plane relative to the tool holder structure 120, and because the extending direction of the first tool tip is located on the first plane, the first tool tip structure 110 rotates relative to the tool holder structure 120 to enable the first tool tip to rotate along the bottom surface of the culture dish, so that the first tool tip can be always attached to the bottom surface of the culture dish in the rotating process of the first tool tip structure 110, and the first tool tip structure 110 can scrape large-area cells once, thereby effectively improving the working efficiency.

An adjustment structure 130 is coupled to the handle structure 120 and the first head structure 110, the adjustment structure 130 being configured to control the rotation of the first head structure 110 relative to the handle structure 120. Specifically, the adjustment structure 130 can be adjusted in a manual adjustment manner, and when a user performs a cell scraping operation, the user only needs to manually operate the adjustment structure 130 to control the rotation of the first tool bit structure 110 relative to the tool bit structure 120.

The cell scraper 100 includes: the cell scraping tool comprises a first tool nose structure 110, a tool shank structure 120 and an adjusting structure 130, wherein the first tool nose structure 110 is provided with a first tool nose, the first tool nose is used for scraping cells, and the extending direction of the first tool nose is positioned on a first plane; the tool shank structure 120 is rotatably connected to the first tool head structure 110, the first tool head structure 110 rotates around a first direction relative to the tool shank structure 120, and the first direction is perpendicular to the first plane; adjustment structure 130 is connected to handle structure 120 and first head structure 110, and adjustment structure 130 is used to control rotation of first head structure 110 relative to handle structure 120. During operation, the first blade tip of the first blade head structure 110 is firstly attached to the bottom wall of the culture dish, and then the adjusting structure 130 is controlled to control the first blade head structure 110 to rotate relative to the blade handle structure 120, so that large-area cells on the bottom wall of the culture dish can be scraped, and the working efficiency is effectively improved.

In some embodiments of the present application, the cell scraper 100 may further include: a second tip structure 140.

The second bit feature 140 has a second tip for scraping cells. Specifically, the second knife tip can be located at the front end of the second knife head structure 140, and the second knife tip can be in a linear shape, so as to ensure that the whole knife tip can be attached to the bottom wall of the culture dish. The extension direction of the second tool tip is positioned in the first plane. In particular, the first plane may be a bottom wall of the culture dish, and in case the culture dish is placed on a horizontal plane, the first plane may also be a horizontal plane.

Handle structure 120 is pivotally connected to second tip structure 140. Specifically, an end of the handle structure 120 is rotatably connected to an end of the second bit structure 140, and the second bit structure 140 can rotate relative to the handle structure 120. The direction of rotation of second tool tip structure 140 relative to handle structure 120 can be a second direction, and the second direction is perpendicular to the first plane. Under the condition that the first plane is a horizontal plane, the second direction is a vertical direction, that is, the second tool bit structure 140 rotates on the horizontal plane relative to the handle structure 120, and because the extending direction of the second tool tip is located on the first plane, the second tool bit structure 140 rotates relative to the handle structure 120 to enable the second tool tip to rotate along the bottom surface of the culture dish, so that the second tool bit structure 140 can be always attached to the bottom surface of the culture dish in the rotating process, and the second tool tip structure 140 can scrape large-area cells once, thereby effectively improving the working efficiency.

Adjustment feature 130 is coupled to handle structure 120 and second tip feature 140, and adjustment feature 130 is configured to control rotation of second tip feature 140 relative to handle structure 120. Specifically, the adjustment structure 130 can be adjusted in a manual adjustment manner, and when a user performs a cell scraping operation, the user only needs to manually operate the adjustment structure 130 to control the rotation of the second tool bit structure 140 relative to the handle structure 120.

Because the first cutter head structure 110 and the second cutter head structure 140 exist at the same time, the adjusting structure 130 can be used for controlling the first cutter head structure 110 and the second cutter head structure 140 to rotate synchronously, so that the first cutter head structure 110 and the second cutter head structure 140 can synchronously scrape cells in different areas, and the working efficiency is further improved.

In some embodiments of the present application, the first head structure 110 is disposed on one side (e.g., left side) of the handle structure 120, the second head structure 140 is disposed on another side (e.g., right side) of the handle structure 120 facing away from the first head structure 110, and the first direction and the second direction are opposite directions. In an initial state, the first tool bit structure 110 and the second tool bit structure 140 can be tightly attached together, and at this time, the first tool bit and the second tool bit are both attached to the bottom wall of the culture dish, and then the first tool bit structure 110 is rotated towards the left side and the second tool bit structure 140 is rotated towards the right side by controlling the adjusting structure 130, so that the first tool bit structure 110 and the second tool bit structure 140 can respectively scrape cells at different positions, and the aim of further improving the working efficiency is achieved.

In some embodiments of the present application, the adjustment structure 130 may include: a first gear 131 and a lever 132.

The first gear 131 is fixedly connected to the first head structure 110. Specifically, the first gear 131 may be fixedly connected to the first tool bit structure 110 by welding, bonding, or the like, so as to ensure the connection strength of the two; the first gear 131 may also be fixedly connected to the first head structure 110 by a screw, a buckle, or the like, so as to facilitate the assembly and disassembly of the two. Specifically, in the embodiment of the present application, the first gear 131 and the first tool bit structure 110 may be an integrally formed structure to ensure the connection strength therebetween and facilitate manufacturing.

The lever 132 has a first sidewall forming a first rack 135, the first rack 135 is engaged with the first gear 131, and the lever 132 is slidably coupled to the handle structure 120. By manipulating the control rod 132 to move relative to the handle structure 120, the first gear 131 can be controlled to rotate, and the first head structure 110 can be controlled to rotate relative to the handle structure 120.

In some embodiments of the present application, the adjustment structure 130 may further include: a second gear 136.

The second gear 136 is fixedly coupled to the second bit structure 140. Specifically, the second gear 136 may be fixedly connected to the second cutter head structure 140 by welding, bonding, or the like, so as to ensure the connection strength of the two; the second gear 136 can also be fixedly connected to the second bit structure 140 by screws, snaps, or the like, to facilitate the assembly and disassembly of the two. Specifically, in the embodiment of the present application, the second gear 136 and the second bit structure 140 may be an integrally formed structure to ensure the connection strength therebetween and facilitate the manufacturing.

The control lever 132 has a second side wall forming a second rack 137, the second rack 137 is in meshing engagement with the second gear 136, and the control lever 132 is slidably connected to the handle structure 120. By manipulating the control rod 132 to move relative to the handle structure 120, the second gear 136 and, therefore, the second bit structure 140 can be controlled to rotate relative to the handle structure 120. Specifically, the second sidewall and the first sidewall are disposed opposite to each other, the second sidewall may be disposed on the right side of the control lever 132, and the first sidewall may be disposed on the left side of the control lever 132.

In some embodiments of the present application, the adjustment structure 130 may further include: the elastic member 138.

One end of the elastic member 138 is fixedly connected to the handle structure 120, the other end of the elastic member 138 opposite to the one end is fixedly connected to the control rod 132, and the elastic member 138 is used for driving the control rod 132 to move in a direction away from the first gear 131. With the elastic member 138 kept in the initial state, the control lever 132 is at the farthest position from the first gear 131, and both the first gear 131 and the second gear 136 are kept in the state of tightly fitting the first bit structure 110 and the second bit structure 140, and in the case of non-use, the first bit structure 110 and the second bit structure 140 can be tightly fitted by the elastic member 138 so as to be received.

In some embodiments of the present application, the lever 132 includes a main lever 133 and a control block 134.

The main lever 133 is fixedly connected to the control block 134, and the control block 134 protrudes from the main lever 133. Specifically, the control block 134 may have a block shape, and the protruding direction of the control block 134 may be toward the side of the main lever 133.

The handle structure 120 has an opening therein, the opening is communicated with the accommodating cavity, the main rod 133 is located in the accommodating cavity, and the control block 134 has an opening extending out of the accommodating cavity. In the case where the user needs to manually operate the control lever 132, the control block 134 extending from the receiving chamber is manually operated. In particular, during use, the user need only push the control block 134 with a finger to control the movement of the control lever 132.

In some embodiments of the present application, the first tool bit structure 110 may include: a first radial arm 111 and a first blade 112.

First radial arm 111 is pivotally connected to handle structure 120 and first blade 112 has a first mounting slot therein. The first radial arm 111 is partially inserted into the first mounting groove, and the first blade 112 is detachably connected to the first radial arm 111. Before use, the first blade 112 and the first radial arm 111 are in a separated state, and a user only needs to insert the first radial arm 111 into the first mounting groove of the clean first blade 112, so that the first blade 112 can be fixedly connected to the first mounting groove, and the cell scraping work can be completed. After scraping the cells from one culture dish, the first used blade 112 is simply removed and replaced with the first clean blade 112, and the next culture dish can be scraped.

In some embodiments of the present application, the second bit structure 140 may include: a second radial arm 141 and a second blade 142.

The second swing arm 141 is rotatably connected to the handle structure 120, and the second blade 142 has a second mounting groove. The second radial arm 141 is partially inserted into the second mounting groove, and the second blade 142 is detachably connected to the second radial arm 141. Before the use, the second blade 142 and the second radial arm 141 are in a separated state, and the user only needs to insert the second radial arm 141 into the second mounting groove of the clean second blade 142, so that the second blade 142 is fixedly connected to the second mounting groove, and the cell scraping work can be completed. After scraping the cells from one culture dish, the second used blade 142 is detached and replaced with the second clean blade 142, so that the next culture dish can be scraped.

In some embodiments of the present application, the cell scraper 100 may further include: a storage box 150.

The inside of the receiving case 150 forms a receiving space for receiving the first and

second blades

112 and 142. Specifically, the first blade 112 and the second blade 142 may be provided in plurality, and the plurality of first blades 112 and the plurality of second blades 142 may be of the same specification, so that the first blade 112 and the second blade 142 are used in combination.

The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A cell scraper, comprising:

2. The cell scraper of claim 1, further comprising:

3. The cell scraper of claim 2, wherein the first blade head structure is disposed on one side of the handle structure and the second blade head structure is disposed on another side of the handle structure facing away from the first blade head structure, the second direction being opposite the first direction.

4. The cell scraper of claim 3, wherein the regulating structure comprises:

the first gear is fixedly connected to the first tool bit structure, and a rotating shaft of the first gear is collinear with a rotating shaft of the first tool bit structure;

5. The cell scraper of claim 4, wherein the regulating structure further comprises:

6. The cell scraper of claim 4, wherein the regulating structure further comprises:

7. The cell scraper of claim 4, wherein the control rod comprises a main rod and a control block, the main rod is fixedly connected to the control block, and the control block protrudes from the main rod;

8. The cell scraper of claim 2, wherein the first blade structure comprises:

the first rotating arm is rotatably connected to the cutter handle structure;

9. The cell scraper of claim 8, wherein the second blade feature comprises:

the second rotating arm is rotatably connected to the cutter handle structure;

the second blade is provided with a second mounting groove, the second spiral arm part is inserted in the second mounting groove, and the second blade is detachably connected to the second spiral arm.

10. The cell scraper of claim 9, further comprising:

a receiving box having a receiving space formed therein for receiving the first and second blades.