CN114605693A - Micro-mechanical measurement fixing device based on polyether-ether-ketone and preparation method thereof - Google Patents
Micro-mechanical measurement fixing device based on polyether-ether-ketone and preparation method thereof Download PDFInfo
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- CN114605693A CN114605693A CN202210238179.9A CN202210238179A CN114605693A CN 114605693 A CN114605693 A CN 114605693A CN 202210238179 A CN202210238179 A CN 202210238179A CN 114605693 A CN114605693 A CN 114605693A
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- sheet
- polyetheretherketone
- ether
- polyether
- ketone
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- 239000004696 Poly ether ether ketone Substances 0.000 title claims abstract description 95
- 229920002530 polyetherether ketone Polymers 0.000 title claims abstract description 95
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 238000011326 mechanical measurement Methods 0.000 title description 5
- 238000005259 measurement Methods 0.000 claims abstract description 21
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 13
- 108010039918 Polylysine Proteins 0.000 claims description 11
- 229920001690 polydopamine Polymers 0.000 claims description 11
- 229920000656 polylysine Polymers 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 9
- 238000009210 therapy by ultrasound Methods 0.000 claims description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical compound OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 239000003755 preservative agent Substances 0.000 claims description 3
- 230000002335 preservative effect Effects 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 6
- 239000000523 sample Substances 0.000 description 15
- 210000001519 tissue Anatomy 0.000 description 13
- 239000000758 substrate Substances 0.000 description 8
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 230000020477 pH reduction Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- XOJVVFBFDXDTEG-UHFFFAOYSA-N Norphytane Natural products CC(C)CCCC(C)CCCC(C)CCCC(C)C XOJVVFBFDXDTEG-UHFFFAOYSA-N 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 210000001188 articular cartilage Anatomy 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001643 poly(ether ketone) Polymers 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000004506 ultrasonic cleaning Methods 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/12—Chemical modification
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/12—Chemical modification
- C08J7/123—Treatment by wave energy or particle radiation
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/12—Chemical modification
- C08J7/14—Chemical modification with acids, their salts or anhydrides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N3/00—Investigating strength properties of solid materials by application of mechanical stress
- G01N3/02—Details
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2361/00—Characterised by the use of condensation polymers of aldehydes or ketones; Derivatives of such polymers
- C08J2361/04—Condensation polymers of aldehydes or ketones with phenols only
- C08J2361/16—Condensation polymers of aldehydes or ketones with phenols only of ketones with phenols
Abstract
The invention discloses a micromechanics measurement fixing device based on polyether-ether-ketone and a preparation method thereof. The invention has the advantages of convenient operation, time and labor saving, firm fixation, no damage to samples and the like, and better meets the requirements of the biological tissue sample detection on accuracy, rapidity, reusability and the like.
Description
Technical Field
The invention relates to a fixing platform of a biological tissue sample, in particular to a micromechanics measurement fixing device based on polyether-ether-ketone and a preparation method thereof.
Background
In the prior art, a biomechanical measuring device such as a micrometer indenter and an atomic force microscope is generally used for detecting biological samples such as articular cartilage, and in the detection process, in order to fix the biological tissue samples, glue (502 glue, AB glue and the like) or mechanical fixing and other methods are mainly used.
Disclosure of Invention
The invention aims to solve the technical problem of providing a micro-mechanical measurement fixing device based on polyether-ether-ketone and a preparation method thereof, aiming at the defects of the prior art, wherein the micro-mechanical measurement fixing device has strong adhesive force on a biological tissue sample, is convenient to operate, saves time and labor, is firm in fixation, and does not damage the sample.
In order to solve the technical problems, the invention adopts the following technical scheme.
A micromechanics measurement fixing device based on polyether-ether-ketone comprises a polyether-ether-ketone sheet and polycation molecules adsorbed on the polyether-ether-ketone sheet.
Preferably, the polycationic molecule comprises polylysine or polydopamine.
Preferably, the polyetheretherketone sheet is a square sheet.
Preferably, the polyetheretherketone sheet has dimensions of 1.0cm by 0.5 cm.
A preparation method of a micromechanics measurement fixing device based on polyether-ether-ketone comprises the following steps: step S1, preparing a polyetheretherketone sheet, placing the polyetheretherketone sheet in acetone, ethanol or deionized water for continuous ultrasonic treatment for a preset time, and then drying; step S2, soaking the polyetheretherketone sheet with concentrated sulfuric acid, then cleaning the concentrated sulfuric acid remaining on the polyetheretherketone, and then treating the surface of the polyetheretherketone sheet with oxygen plasma to further activate the surface of the polyetheretherketone sheet; step S3, soaking the polyether-ether-ketone sheet in polycation molecule solution for a preset time to ensure that the polycation molecules are fully contacted with the polyether-ether-ketone sheet; and step S4, modifying the polyether-ether-ketone sheet with adsorbed polycation molecules, then freeze-drying, and finally sealing and storing the polyether-ether-ketone sheet by using a preservative film.
Preferably, in step S1, the polyetheretherketone sheet is subjected to continuous ultrasonic treatment for 10 minutes, and then to drying treatment using an air pump.
Preferably, in step S2, the polyetheretherketone sheet is soaked in 98% concentrated sulfuric acid for 3 minutes, then placed in deionized water for ultrasonic treatment for 10 minutes, and then the surface of the polyetheretherketone sheet is treated with oxygen plasma for 3 minutes.
Preferably, in step S3, the polycationic molecule solution includes polylysine or polydopamine.
Preferably, in the step S4, the concentration of the polydopamine is 160mg/mL to 340 mg/mL.
Preferably, in the step S4, the polylysine has a concentration of 50mg/mL to 100 mg/mL.
The invention discloses a micromechanics measurement fixing device based on polyether-ether-ketone and a preparation method thereof. Compared with the prior art, the invention adopts the polyether-ether-ketone substrate with higher hardness and certain plasticity as the fixing platform of the biological sample, and the polycation molecules are adsorbed on the polyether-ether-ketone substrate, so that the adsorption capacity of the surface of the polyether-ether-ketone substrate on the biological tissue sample and the sample pool is increased, the polyether-ether-ketone substrate has the excellent characteristics of convenient operation, time saving, labor saving, firm fixation, no damage to the sample and the like, and the requirements of the detection work of the biological tissue sample on accuracy, rapidity, reusability and the like are better met.
Drawings
FIG. 1 is a flow chart of a method for preparing a micromechanical measurement fixture according to the present invention;
FIG. 2 is a photograph of a polyetheretherketone sheet in its pristine condition;
FIG. 3 is a raw state micrograph of a polyetheretherketone sheet;
FIG. 4 is a photograph of a PEEK sheet after acidification;
FIG. 5 is a microscopic image of a PEEK sheet after acidification;
FIG. 6 is a photograph of a PEEK sheet having polycationic molecules adsorbed thereon;
FIG. 7 is a schematic diagram of a poly (ether-ketone) sheet with adsorbed polycation molecules;
FIG. 8 is a graph of experimental data for a sample of biological tissue carried by a micromechanical measurement fixture according to the present invention;
FIG. 9 is a graph of experimental data for carrying a biological tissue sample using a prior art means.
Detailed Description
The invention is described in more detail below with reference to the figures and examples.
The invention discloses a micromechanics measurement fixing device based on polyether-ether-ketone, which comprises a polyether-ether-ketone sheet and polycation molecules adsorbed on the polyether-ether-ketone sheet.
In the preparation process of the device, firstly, the polyetheretherketone sheet is subjected to continuous ultrasonic cleaning and drying treatment, then the polyetheretherketone sheet is soaked in concentrated sulfuric acid, after the concentrated sulfuric acid remaining on the polyetheretherketone is cleaned, the surface of the polyetheretherketone sheet is treated by oxygen plasma to activate the surface of the polyetheretherketone sheet, then the polyetheretherketone sheet is soaked in polycation molecular solution to make the polycation molecules fully contact with the polyetheretherketone sheet, and finally, the polyetheretherketone sheet with the polycation molecules attached thereto is modified, freeze-dried and then sealed for storage. Compared with the prior art, the invention adopts the polyether-ether-ketone substrate with higher hardness and certain plasticity as the fixing platform of the biological sample, and the polycation molecules are adsorbed on the polyether-ether-ketone substrate, so that the adsorption capacity of the surface of the polyether-ether-ketone substrate to the biological tissue sample and a sample pool is increased, the polyether-ether-ketone substrate has the excellent characteristics of convenient operation, time and labor saving, firm fixation, no damage to the sample and the like, and the requirements of the detection work of the biological tissue sample on accuracy, rapidity, reusability and the like are better met.
In this embodiment, the polycationic molecule comprises polylysine or polydopamine.
Preferably, the polyetheretherketone sheet is a square sheet. Specifically, the dimensions of the polyetheretherketone sheet are 1.0cm × 1.0cm × 0.5 cm.
In order to better describe the technical scheme of the present invention, the present invention further provides a preparation method of a micromechanical measurement fixing device based on polyetheretherketone, please refer to fig. 1, which comprises the following steps:
step S1, preparing a polyetheretherketone sheet, placing the polyetheretherketone sheet in acetone, ethanol or deionized water for continuous ultrasonic treatment for a preset time, and then drying; see fig. 2 and fig. 3 for a treated peek sheet;
step S2, soaking the polyetheretherketone sheet with concentrated sulfuric acid, then cleaning the concentrated sulfuric acid remaining on the polyetheretherketone, and then treating the surface of the polyetheretherketone sheet with oxygen plasma to further activate the surface of the polyetheretherketone sheet; see fig. 4 and 5 for a treated peek sheet;
step S3, soaking the polyether-ether-ketone sheet in polycation molecule solution for a preset time to ensure that the polycation molecules are fully contacted with the polyether-ether-ketone sheet; see fig. 6 and 7 for a treated peek sheet;
and step S4, modifying the polyether-ether-ketone sheet with adsorbed polycation molecules, then freeze-drying, and finally sealing and storing the polyether-ether-ketone sheet by using a preservative film.
In the preparation method, the molecules of polycations such as polylysine, polydopamine and the like can interact with anions on biological tissues to generate stronger adhesive force, so that the invention adopts polyether-ether-ketone with higher hardness as a base material, and the molecules of the polycations as an adhesive to fix large biological tissues in the micromechanics measurement and measurement process. In practical application, the preparation method can adopt the following specific steps:
in step S1, the polyetheretherketone sheet is subjected to continuous ultrasonic treatment for 10 minutes, and then dried with an air pump;
in step S2, in order to sufficiently remove the residual sulfuric acid on the polyetheretherketone, the polyetheretherketone sheet is immersed in 98% concentrated sulfuric acid for 3 minutes, then placed in deionized water for ultrasonic treatment for 10 minutes, and then the surface of the polyetheretherketone sheet is treated with oxygen plasma (0.01Torr) for 3 minutes;
in step S3, the polycationic molecule solution includes polylysine or polydopamine;
in the step S4, the concentration of the polydopamine is 160 mg/mL-340 mg/mL;
in the step S4, the polylysine has a concentration of 50 to 100 mg/mL.
In particular, reference is made to the following table for specific parameter settings of said polydopamine and said polylysine:
the comparative test data of the polyether-ether-ketone sheet adsorbed with polycation molecules and the conventional biological tissue sample fixing means are shown in fig. 8 and 9, and as can be clearly seen from fig. 8, the micro-mechanical measurement fixing device prepared by the method can make the obtained detection data more stable, make the detection work of the biological tissue sample more accurate and rapid, and better meet the application requirements.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents or improvements made within the technical scope of the present invention should be included in the scope of the present invention.
Claims (10)
1. A micromechanics measurement fixing device based on polyether-ether-ketone is characterized by comprising a polyether-ether-ketone sheet and polycation molecules adsorbed on the polyether-ether-ketone sheet.
2. The polyetheretherketone-based micromechanical measurement fixture of claim 1, wherein the polycation molecule comprises polylysine or polydopamine.
3. The polyetheretherketone-based micromechanical measurement fixture of claim 1, wherein the polyetheretherketone sheet is a square sheet.
4. The polyetheretherketone-based micromechanical measurement fixture of claim 3, wherein the polyetheretherketone sheet has dimensions of 1.0cm x 0.5 cm.
5. A preparation method of a micromechanics measurement fixing device based on polyether-ether-ketone is characterized by comprising the following steps:
step S1, preparing a polyetheretherketone sheet, placing the polyetheretherketone sheet in acetone, ethanol or deionized water for continuous ultrasonic treatment for a preset time, and then drying;
step S2, soaking the polyetheretherketone sheet with concentrated sulfuric acid, then cleaning the concentrated sulfuric acid remaining on the polyetheretherketone, and then treating the surface of the polyetheretherketone sheet with oxygen plasma to further activate the surface of the polyetheretherketone sheet;
step S3, soaking the polyether-ether-ketone sheet in polycation molecule solution for a preset time to ensure that the polycation molecules are fully contacted with the polyether-ether-ketone sheet;
and step S4, modifying the polyether-ether-ketone sheet with adsorbed polycation molecules, then freeze-drying, and finally sealing and storing the polyether-ether-ketone sheet by using a preservative film.
6. The method of manufacturing a polyetheretherketone-based micromechanical measurement fixture according to claim 5, wherein in step S1, the polyetheretherketone sheet is continuously sonicated for 10 minutes, followed by drying with an air pump.
7. The method for preparing the PEEK-based micromechanical measurement fixture of claim 5, wherein in step S2, the PEEK sheet is soaked in 98% concentrated sulfuric acid for 3 minutes, then placed in deionized water for 10 minutes of ultrasonic treatment, and then the surface of the PEEK sheet is treated with oxygen plasma for 3 minutes.
8. The method for preparing a polyetheretherketone-based micromechanical measurement fixture of claim 5, wherein in step S3, the polycation molecular solution comprises polylysine or polydopamine.
9. The method for preparing a polyetheretherketone-based micromechanical measurement fixture of claim 8, wherein in step S4, the concentration of the polydopamine is 160mg/mL to 340 mg/mL.
10. The method for preparing a polyetheretherketone-based micromechanical measurement fixture according to claim 8, wherein in step S4, the polylysine is present in a concentration of 50mg/mL to 100 mg/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210238179.9A CN114605693A (en) | 2022-03-11 | 2022-03-11 | Micro-mechanical measurement fixing device based on polyether-ether-ketone and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210238179.9A CN114605693A (en) | 2022-03-11 | 2022-03-11 | Micro-mechanical measurement fixing device based on polyether-ether-ketone and preparation method thereof |
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| CN114605693A true CN114605693A (en) | 2022-06-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210238179.9A Pending CN114605693A (en) | 2022-03-11 | 2022-03-11 | Micro-mechanical measurement fixing device based on polyether-ether-ketone and preparation method thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101408496A (en) * | 2008-11-18 | 2009-04-15 | 中国检验检疫科学研究院 | Method for performing biological mark detection by atomic force microscope |
| CN103517806A (en) * | 2011-05-12 | 2014-01-15 | 日立化成株式会社 | Thin film with base and method for producing same |
-
2022
- 2022-03-11 CN CN202210238179.9A patent/CN114605693A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101408496A (en) * | 2008-11-18 | 2009-04-15 | 中国检验检疫科学研究院 | Method for performing biological mark detection by atomic force microscope |
| CN103517806A (en) * | 2011-05-12 | 2014-01-15 | 日立化成株式会社 | Thin film with base and method for producing same |
Non-Patent Citations (2)
| Title |
|---|
| AN\"AN SUNA ET.AL.: "Facile surface functional polyetheretherketone with antibacterial and immunoregulatory activities for enhanced regeneration toward bacterium-infected bone destruction", 《DRUG DELIVERY》 * |
| 王娜: "聚多巴胺修饰磺化聚醚醚酮的制备及性能研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
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Application publication date: 20220610 |
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