CN114588317A - Flexible inorganic nanofiber composite scaffold and preparation method thereof - Google Patents
Flexible inorganic nanofiber composite scaffold and preparation method thereof Download PDFInfo
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- CN114588317A CN114588317A CN202210225494.8A CN202210225494A CN114588317A CN 114588317 A CN114588317 A CN 114588317A CN 202210225494 A CN202210225494 A CN 202210225494A CN 114588317 A CN114588317 A CN 114588317A
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Images
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
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- Spinning Methods And Devices For Manufacturing Artificial Fibers (AREA)
Abstract
The invention provides a flexible inorganic nano-fiber composite bracket and a preparation method thereof, wherein the preparation method comprises the following steps: preparing a precursor solution; adding antibacterial salt into the precursor solution to obtain a salt-doped mixed solution; preparing a template agent aqueous solution; mixing the mixed solution doped with salt and a template agent aqueous solution to obtain an electrostatic spinning solution; spinning the electrostatic spinning solution to obtain a nanofiber membrane; and calcining the nanofiber membrane to obtain the flexible inorganic nanofiber composite support. According to the preparation method of the flexible inorganic nanofiber composite scaffold provided by the invention, inorganic components are used as raw materials, the prepared nanofiber composite scaffold does not contain organic components, compared with the existing organic nanofiber scaffold, the degradation speed is slow, and antibacterial components can be released for a long time, so that the prepared flexible inorganic nanofiber composite scaffold can keep long-term biological activity, and the anti-inflammatory and antibacterial effects of the flexible inorganic nanofiber composite scaffold are longer in duration.
Description
Technical Field
The invention relates to the technical field of biological materials, in particular to a flexible inorganic nanofiber composite scaffold and a preparation method thereof.
Background
The tissue engineering scaffold is a place where cells depend on survival, proliferation and differentiation, wherein the electrostatic spinning technology is an effective means for preparing the tissue engineering scaffold, and the tissue engineering scaffold has the advantages of simple and efficient preparation method, capability of preparing fibers with diameters ranging from nanometer to micrometer, capability of simulating natural extracellular matrix and the like.
In the field of tissue engineering repair of skin wounds, effective inhibition of bacterial growth during the repair of wounds is of great importance for timely repair of wounds. The breeding of bacteria can cause inflammation, wound ulceration, tissue necrosis, and delay wound healing. The copper ions have certain anti-inflammatory and antibacterial effects and have excellent effect of inhibiting bacterial growth in the field of wound repair, so that the copper ions have wide application prospects in the field of biomedical skin wound repair.
In the existing tissue engineering scaffold, a common spinning solution of an electrostatic spinning technology is a high molecular organic compound including PLLA, PCL, PLGA and the like, or a natural high molecular material including gelatin, silk fibroin and the like, so that the obtained nanofiber scaffold is an organic nanofiber scaffold.
Because the organic nano fiber scaffold is degraded quickly, the copper ions in the organic nano fiber scaffold used in the field of wound repair are released quickly, so that the duration of the anti-inflammatory and antibacterial effects of the nano fiber scaffold is short.
Disclosure of Invention
The invention solves the problem that the existing nanofiber scaffold used in the field of wound surface repair has short duration of anti-inflammatory and antibacterial effects.
In order to solve the problems, the invention provides a preparation method of a flexible inorganic nanofiber composite scaffold, which comprises the following steps:
s1: preparing a precursor solution by taking tetraethoxysilane, deionized water and phosphoric acid as raw materials;
s2: adding antibacterial salt into the precursor solution, and mixing and stirring at room temperature to obtain a salt-doped mixed solution;
s3: preparing a template agent aqueous solution by using polyvinyl alcohol as a template agent;
s4: mixing the salt-doped mixed solution with the template agent aqueous solution, and stirring at room temperature to obtain an electrostatic spinning solution;
s5: spinning the electrostatic spinning solution by using an electrostatic spinning process to obtain a nanofiber membrane;
s6: and calcining the nanofiber membrane to obtain the flexible inorganic nanofiber composite support.
Optionally, the antibacterial salt is selected from at least one of copper salts and zinc salts.
Optionally, the copper salt is selected from at least one of copper chloride and copper nitrate.
Optionally, the zinc salt comprises zinc chloride.
Optionally, the mass ratio of the antibacterial salt to the precursor solution ranges from (0.005-0.01): 1.
optionally, with polyvinyl alcohol as a template, preparing the aqueous solution of the template comprises: and (3) mixing polyvinyl alcohol with water according to the mass ratio of 1:9, and stirring for 24 hours at room temperature to obtain the template agent aqueous solution.
Optionally, the mass ratio of the salt-doped mixed solution to the template aqueous solution is 1: 1.
Optionally, spinning the electrospun liquid using an electrospinning process comprises: and (3) utilizing an electrostatic spinning process, setting the electrostatic spinning high voltage to be 20kV, setting the propelling speed of a propelling pump to be 2mL/h, and collecting to obtain the nanofiber membrane by taking non-woven fabrics as a receiving device.
Optionally, calcining the nanofiber membrane comprises: and placing the nanofiber membrane in a muffle furnace, and calcining for 1-3 h at 700-900 ℃ to obtain the flexible inorganic nanofiber composite support.
Another object of the present invention is to provide a flexible inorganic nanofiber composite scaffold prepared by the method for preparing the flexible inorganic nanofiber composite scaffold as described above.
Compared with the prior art, the preparation method of the flexible inorganic nanofiber composite scaffold provided by the invention has the following advantages:
according to the preparation method of the flexible inorganic nanofiber composite scaffold provided by the invention, inorganic components are used as raw materials, and the prepared nanofiber composite scaffold has remarkable flexibility; the flexible inorganic nanofiber composite scaffold does not contain organic components, compared with the existing organic nanofiber scaffold, the flexible inorganic nanofiber composite scaffold is slow in degradation speed and capable of releasing antibacterial components for a long time, so that the prepared flexible inorganic nanofiber composite scaffold can keep long-term bioactivity, and the duration time of the anti-inflammatory antibacterial action of the flexible inorganic nanofiber composite scaffold is long.
Drawings
Fig. 1 is an SEM image and a diameter distribution diagram (n is 100) of the copper-doped flexible inorganic nanofiber composite scaffold according to the present invention;
FIG. 2 is a diagram showing the antibacterial effect of the copper-doped flexible inorganic nanofiber composite scaffold of the present invention;
FIG. 3 is a diagram showing the cell proliferation activity of the NIH3T3 cells planted on the copper-doped flexible inorganic nanofiber composite scaffold at various time points (1 day, 4 days and 7 days);
FIG. 4 is a cell electron microscope image of NIH3T3 cells planted on the copper-doped flexible inorganic nanofiber composite scaffold at each time point (1 day, 4 days, 7 days);
FIG. 5 is an inverted fluorescence microscope image of NIH3T3 cells planted on the copper-doped flexible inorganic nanofiber composite scaffold at various time points (1 day, 4 days, 7 days).
Detailed Description
The following describes embodiments of the present invention in detail. The embodiments described below with reference to the drawings are exemplary and intended to be illustrative of the present invention and should not be construed as limiting the present invention, and all other embodiments that can be obtained by one skilled in the art based on the embodiments of the present invention without inventive efforts shall fall within the scope of protection of the present invention.
In order to solve the problem that the existing nanofiber scaffold used in the field of wound surface repair has short duration of anti-inflammatory and antibacterial effects, the invention provides a preparation method of a flexible inorganic nanofiber composite scaffold, which comprises the following steps:
s1: preparing a precursor solution by taking tetraethoxysilane, deionized water and phosphoric acid as raw materials;
s2: adding antibacterial salt into the precursor solution, and mixing and stirring at room temperature to obtain a salt-doped mixed solution;
s3: preparing a template agent aqueous solution by using polyvinyl alcohol as a template agent;
s4: mixing the mixed solution doped with salt with the template agent aqueous solution, and stirring at room temperature to obtain an electrostatic spinning solution;
s5: spinning the electrostatic spinning solution by using an electrostatic spinning process to obtain a nanofiber membrane;
s6: and calcining the nanofiber membrane to obtain the flexible inorganic nanofiber composite support.
The SiO is obtained by mixing tetraethoxysilane, deionized water and phosphoric acid2Sol solution of the SiO2The sol solution is a precursor solution; antibacterial salt is further added into the precursor solution, so that the prepared flexible inorganic nanofiber composite scaffold has antibacterial and anti-inflammatory effects; the preparation method comprises the following steps of mixing and stirring a prepared polyvinyl alcohol aqueous solution and a salt-doped mixed solution by taking polyvinyl alcohol as a template agent to obtain an electrostatic spinning solution containing antibacterial salt; and spinning the electrostatic spinning solution to obtain a nanofiber membrane formed by accumulating nanofibers, and further calcining the nanofiber membrane obtained by spinning to obtain the nanofiber composite scaffold.
According to the preparation method of the flexible inorganic nanofiber composite scaffold provided by the invention, inorganic components are used as raw materials, and the prepared nanofiber composite scaffold has remarkable flexibility; the flexible inorganic nanofiber composite scaffold does not contain organic components, compared with the existing organic nanofiber scaffold, the flexible inorganic nanofiber composite scaffold is slow in degradation speed and capable of releasing antibacterial components for a long time, so that the prepared flexible inorganic nanofiber composite scaffold can keep long-term bioactivity, and the duration time of the anti-inflammatory antibacterial action of the flexible inorganic nanofiber composite scaffold is long.
According to the preparation method of the flexible inorganic nanofiber composite scaffold provided by the invention, after the nanofiber membrane obtained by electrostatic spinning is calcined, the breakage of fibers can not occur, and the complete membrane structure is still kept, so that the prepared flexible inorganic nanofiber composite scaffold has good mechanical properties.
In order to ensure the antibacterial performance of the flexible inorganic nanofiber composite scaffold, the antibacterial salt is preferably selected from at least one of copper salt and zinc salt.
Copper salt and zinc salt are selected as antibacterial salt in the flexible inorganic nanofiber composite support, so that the flexible inorganic nanofiber composite support can slowly release copper ions or zinc ions, and the copper ions or the zinc ions have positive charges and have the effect of inhibiting bacterial activity, so that the flexible inorganic nanofiber composite support has remarkable antibacterial performance, and the flexible inorganic nanofiber composite support has wide application in the field of wound dressings.
The copper salt is preferably selected from at least one of copper chloride and copper nitrate.
Since the application uses SiO2The sol solution is used as a precursor solution, and copper salt is selected as antibacterial salt, so that the flexible inorganic nanofiber composite scaffold can slowly release Si for a long time4+、Cu2+So as to pass through Si on the basis of having antibacterial and anti-inflammatory effects4+And Cu2+The synergistic effect can also promote the regeneration of bones and blood vessels, thereby providing a promising treatment option for the orthopedic application of bone tissue regeneration; in addition, the flexible inorganic nanofiber composite scaffold provided by the application can be combined with other substances for promoting osteoblast proliferation and differentiation, including Ca2+、Sr2+、Mn2+And mixing the components to prepare the nanofiber composite scaffold for the bone tissue engineering repair field.
Preferred zinc salts herein include zinc chloride.
In order to ensure the antibacterial performance, the mass ratio of the antibacterial salt to the precursor solution is preferably (0.005-0.01): 1.
in the preferable step S1 of the present application, the preparation of the precursor solution using tetraethoxysilane, deionized water, and phosphoric acid as raw materials comprises: mixing ethyl orthosilicate, deionized water and phosphoric acid in a molar ratio of 1:11:0.01, and stirring for 24 hours to obtain a precursor solution.
The preparation of the template aqueous solution by using polyvinyl alcohol as a template comprises the following steps: and (3) mixing polyvinyl alcohol with water in a mass ratio of 1:9, and stirring at room temperature for 24 hours to obtain a template agent aqueous solution.
The mass ratio of the salt-doped mixed solution to the template agent aqueous solution is preferably 1: 1.
The spinning of the electrospinning solution by using the electrospinning process comprises: and (3) utilizing an electrostatic spinning process, setting the electrostatic spinning high voltage to be 20kV, setting the propelling speed of a propelling pump to be 2mL/h, and collecting to obtain the nanofiber membrane by taking non-woven fabrics as a receiving device.
Calcining the nanofiber membrane comprises: and placing the nanofiber membrane in a muffle furnace, and calcining for 1-3 h at 700-900 ℃ to obtain the flexible inorganic nanofiber composite support.
Another object of the present invention is to provide a flexible inorganic nanofiber composite scaffold prepared by the method for preparing a flexible inorganic nanofiber composite scaffold as described above.
The flexible inorganic nanofiber composite scaffold provided by the invention takes inorganic components as raw materials, and has remarkable flexibility; the flexible inorganic nanofiber composite scaffold does not contain organic components, compared with the existing organic nanofiber scaffold, the flexible inorganic nanofiber composite scaffold is slow in degradation speed and capable of releasing antibacterial components for a long time, so that the prepared flexible inorganic nanofiber composite scaffold can keep long-term bioactivity, and the duration time of the anti-inflammatory antibacterial action of the flexible inorganic nanofiber composite scaffold is long.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
Example 1
The embodiment provides a preparation method of a flexible inorganic nanofiber composite scaffold, which comprises the following steps:
s1: mixing tetraethoxysilane, deionized water and phosphoric acid in a molar ratio of 1:11:0.01, and stirring for 24 hours to prepare a precursor solution;
s2: adding 50mg of copper chloride into 10g of the precursor solution, and mixing and stirring at room temperature to obtain a copper-doped mixed solution;
s3: dissolving 2g of polyvinyl alcohol in 18g of deionized water, and stirring for 24 hours at room temperature to prepare a template agent aqueous solution;
s4: mixing the copper-doped mixed solution with the template agent aqueous solution in equal mass, and stirring at room temperature to obtain an electrostatic spinning solution;
s5: spinning the electrostatic spinning solution by using an electrostatic spinning process, setting the electrostatic spinning high voltage to be 20kV, setting the propelling speed of a propelling pump to be 2mL/h, and collecting to obtain a nanofiber membrane by using non-woven fabrics as a receiving device;
s6: placing the nanofiber membrane in a muffle furnace, calcining for 2h at 800 ℃, removing the template agent PVA, and obtaining the flexible SiO2-CuO-0.5% nanofiber composite scaffold.
Referring to fig. 1, it can be seen from the SEM image and the diameter distribution diagram that the diameters of the nanofibers of the copper-doped flexible inorganic nanofiber composite scaffold are uniformly distributed in a normal distribution, wherein SiO is in the normal distribution2-CuO-0.5% with an average diameter of 429.09nm, less than 500 nm.
Referring to FIG. 2, the copper-doped flexible inorganic nanofiber composite scaffold and the blank control group and pure SiO without copper are compared2The number of colonies of the nanofiber membrane group against escherichia coli (e.coli) and staphylococcus aureus (s.aureus), it can be seen that SiO2The experimental group of-CuO-0.5% has obvious effect of inhibiting the growth of the bacterial colony for E.coli and S.aureus.
As shown in fig. 3, byThe CCK8 kit incubates the copper-doped flexible inorganic nanofiber composite scaffold at different time points (1 day, 4 days and 7 days), and the absorbance of the copper-doped flexible inorganic nanofiber composite scaffold at 450nm is measured by using an enzyme-labeling instrument so as to reflect the cell proliferation activity of the copper-doped flexible inorganic nanofiber composite scaffold, and SiO can be seen2The proliferation capacity of-CuO-0.5% to NIH3T3 cells is higher than that of the control group, SiO2the-CuO-0.5% experimental group was more favorable for the proliferation of co-cultured NIH3T3 cells on the scaffold.
Referring to fig. 4, SEM photography of the co-cultured cells fixed with 4% paraformaldehyde at different time points (1 day, 4 days, 7 days) was performed by using the copper-doped flexible inorganic nanofiber composite scaffold, and it can be seen that SiO was formed2On day seven, almost complete spreading of NIH3T3 cells on the scaffolds was achieved with-CuO-0.5%, the results being consistent with the data reflected in FIG. 3.
Referring to FIG. 5, by staining live cells and dead cells of NIH3T3 after coculture at different time points (1 day, 4 days, 7 days) on the copper-doped flexible inorganic nanofiber composite scaffolds with fluorescent dyes (AM, PI), SiO can be seen from live and dead fluorescence patterns2The number of live cells in-CuO-0.5% of the experimental groups far exceeds the number of dead cells, reflecting that the composite scaffold has good cell compatibility, and the results are consistent with the data results reflected in FIG. 3.
According to the detection, the flexible inorganic nanofiber composite scaffold provided by the embodiment is slow in degradation speed and capable of releasing antibacterial ingredients for a long time, so that the prepared flexible inorganic nanofiber composite scaffold can keep long-term biological activity, and the duration of the anti-inflammatory and antibacterial effects of the flexible inorganic nanofiber composite scaffold is long.
Example 2
The embodiment provides a preparation method of a flexible inorganic nanofiber composite scaffold, which comprises the following steps:
s1: mixing tetraethoxysilane, deionized water and phosphoric acid in a molar ratio of 1:11:0.01, and stirring for 24 hours to prepare a precursor solution;
s2: adding 100mg of copper chloride into 10g of precursor solution, and mixing and stirring at room temperature to obtain a copper-doped mixed solution;
s3: dissolving 2g of polyvinyl alcohol in 18g of deionized water, and stirring for 24 hours at room temperature to prepare a template agent aqueous solution;
s4: mixing the copper-doped mixed solution with the template agent aqueous solution in equal mass, and stirring at room temperature to obtain an electrostatic spinning solution;
s5: spinning the electrostatic spinning solution by using an electrostatic spinning process, setting the electrostatic spinning high voltage to be 20kV, setting the propelling speed of a propelling pump to be 2mL/h, and collecting to obtain a nanofiber membrane by using non-woven fabrics as a receiving device;
s6: placing the nanofiber membrane in a muffle furnace, calcining for 1h at 900 ℃, removing the template agent PVA, and obtaining the flexible SiO2-CuO-1% nanofiber composite scaffold.
Referring to fig. 1, it can be seen from the SEM image and the diameter distribution diagram that the diameters of the nanofibers of the copper-doped flexible inorganic nanofiber composite scaffold are uniformly distributed in a normal distribution, wherein SiO is in the normal distribution2-CuO-1% has an average diameter of 492.35nm, less than 500 nm.
Referring to FIG. 2, the copper-doped flexible inorganic nanofiber composite scaffold and the blank control group and pure SiO without copper are compared2The colony counts of the nanofiber membrane group against escherichia coli (e.coli) and staphylococcus aureus (s.aureus) were observed, and SiO was observed2the-CuO-1% experimental group has obvious effect of inhibiting the growth of colonies on E.coli and S.aureus.
Referring to fig. 3, the copper-doped flexible inorganic nanofiber composite scaffold was incubated at different time points (1 day, 4 days, 7 days) with CCK8 kit, and the absorbance at 450nm was measured with microplate reader, so as to reflect the cell proliferation activity of the copper-doped flexible inorganic nanofiber composite scaffold, and it can be seen that SiO was present2The capability of-CuO-1% on the proliferation of NIH3T3 cells is higher than that of a control group, SiO2the-CuO-1% experimental group was more favorable for the proliferation of co-cultured NIH3T3 cells on the scaffold.
Referring to fig. 4, SEM photography of the co-cultured cells fixed with 4% paraformaldehyde at different time points (1 day, 4 days, 7 days) was performed by using the copper-doped flexible inorganic nanofiber composite scaffold, and it can be seen that SiO was formed2-CuO-1% inNIH3T3 cells were almost completely spread on the scaffold at day seven, with results consistent with the data reflected in figure 3.
Referring to FIG. 5, by staining live cells and dead cells of NIH3T3 after coculture at different time points (1 day, 4 days, 7 days) on the copper-doped flexible inorganic nanofiber composite scaffolds with fluorescent dyes (AM, PI), SiO can be seen from live and dead fluorescence patterns2The number of live cells in-CuO-1% experimental group far exceeds the number of dead cells, reflecting that the composite scaffold has good cell compatibility, and the results are consistent with the data results reflected in FIG. 3.
According to the detection, the flexible inorganic nanofiber composite scaffold provided by the embodiment is slow in degradation speed and capable of releasing antibacterial ingredients for a long time, so that the prepared flexible inorganic nanofiber composite scaffold can keep long-term biological activity, and the duration of the anti-inflammatory and antibacterial effects of the flexible inorganic nanofiber composite scaffold is long.
Example 3
The embodiment provides a preparation method of a flexible inorganic nanofiber composite scaffold, which comprises the following steps:
s1: mixing tetraethoxysilane, deionized water and phosphoric acid in a molar ratio of 1:11:0.01, and stirring for 24 hours to prepare a precursor solution;
s2: adding 80mg of zinc chloride into 10g of precursor solution, and mixing and stirring at room temperature to obtain a zinc-doped mixed solution;
s3: dissolving 2g of polyvinyl alcohol in 18g of deionized water, and stirring for 24 hours at room temperature to prepare a template agent aqueous solution;
s4: mixing the zinc-doped mixed solution with the template agent aqueous solution in equal mass, and stirring at room temperature to obtain an electrostatic spinning solution;
s5: spinning the electrostatic spinning solution by using an electrostatic spinning process, setting the electrostatic spinning high voltage to be 20kV, setting the propelling speed of a propelling pump to be 2mL/h, and collecting to obtain a nanofiber membrane by using non-woven fabrics as a receiving device;
s6: and (3) placing the nanofiber membrane in a muffle furnace, calcining for 3h at 700 ℃, and removing the template agent PVA to obtain the flexible inorganic nanofiber composite scaffold.
The detection process and the detection result are referred to the relevant contents of the embodiment 1 and the embodiment 2.
Although the present disclosure has been described above, the scope of the present disclosure is not limited thereto. Those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present disclosure, and such changes and modifications will fall within the scope of the present invention.
Claims (10)
1. A preparation method of a flexible inorganic nanofiber composite scaffold is characterized by comprising the following steps:
s1: preparing a precursor solution by taking tetraethoxysilane, deionized water and phosphoric acid as raw materials;
s2: adding antibacterial salt into the precursor solution, and mixing and stirring at room temperature to obtain a salt-doped mixed solution;
s3: preparing a template agent aqueous solution by using polyvinyl alcohol as a template agent;
s4: mixing the salt-doped mixed solution with the template agent aqueous solution, and stirring at room temperature to obtain an electrostatic spinning solution;
s5: spinning the electrostatic spinning solution by using an electrostatic spinning process to obtain a nanofiber membrane;
s6: and calcining the nanofiber membrane to obtain the flexible inorganic nanofiber composite support.
2. The method of claim 1, wherein the antibacterial salt is at least one selected from the group consisting of copper salts and zinc salts.
3. The method of claim 2, wherein the copper salt is at least one selected from the group consisting of copper chloride and copper nitrate.
4. The method of preparing a flexible inorganic nanofiber composite scaffold according to claim 2, wherein said zinc salt comprises zinc chloride.
5. The method for preparing the flexible inorganic nanofiber composite scaffold according to claim 2, wherein the mass ratio of the antibacterial salt to the precursor solution is (0.005-0.01): 1.
6. the method for preparing the flexible inorganic nanofiber composite scaffold as claimed in any one of claims 1 to 5, wherein the preparation of the aqueous solution of the templating agent using polyvinyl alcohol as the templating agent comprises: and (3) mixing polyvinyl alcohol with water according to the mass ratio of 1:9, and stirring for 24 hours at room temperature to obtain the template agent aqueous solution.
7. The method for preparing the flexible inorganic nanofiber composite scaffold as claimed in any one of claims 1 to 5, wherein the mass ratio of the salt-doped mixed solution to the template aqueous solution is 1: 1.
8. The method for preparing the flexible inorganic nanofiber composite scaffold according to any one of claims 1 to 3, wherein the step of spinning the electrospinning solution by using an electrospinning process comprises: and (3) utilizing an electrostatic spinning process, setting the electrostatic spinning high voltage to be 20kV, setting the propelling speed of a propelling pump to be 2mL/h, and collecting to obtain the nanofiber membrane by taking non-woven fabrics as a receiving device.
9. The method of preparing a flexible inorganic nanofiber composite scaffold according to any one of claims 1 to 3, wherein the calcining the nanofiber membrane comprises: and placing the nanofiber membrane in a muffle furnace, and calcining for 1-3 h at 700-900 ℃ to obtain the flexible inorganic nanofiber composite support.
10. A flexible inorganic nanofiber composite scaffold, characterized by being prepared by the method for preparing a flexible inorganic nanofiber composite scaffold according to any one of claims 1 to 9.
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