CN1145793C - Non-destructive supersonic induced-luminessing imaging method and equipment - Google Patents
Non-destructive supersonic induced-luminessing imaging method and equipment Download PDFInfo
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- CN1145793C CN1145793C CNB001172093A CN00117209A CN1145793C CN 1145793 C CN1145793 C CN 1145793C CN B001172093 A CNB001172093 A CN B001172093A CN 00117209 A CN00117209 A CN 00117209A CN 1145793 C CN1145793 C CN 1145793C
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Abstract
The present invention relates to a nondestructive ultrasonic induced luminescence imaging method. The method comprises the procedures: weak ultrasound directly acts on an organism or an organization, and the organism or the organization is induced to generate self endogenous luminescence; an optical receiving assembly converts the luminescence into an electric signal; the electric signal is converted into a digital signal through analog-to-digital conversion; the structural image of the organism or the organization is obtained by processing the digital signal. A nondestructive ultrasonic induced luminescence imaging device is composed of an ultrasonic generating assembly, an optical receiving assembly, an analog-to-digital converter and a computer. The present invention combines the advantages of strong penetrating power of weak ultrasound to organisms and the advantages of high resolution and no damage of optical imaging and is used for diagnosing biologic medical images to obtain high-resolution clear images.
Description
Technical field
The present invention relates to a kind of medical imaging diagnosis technology, it is luminous from the body endogenous to biosome harmless weak supersonic induced biosome or tissue generation to be particularly related to a kind of utilization, and utilizes luminous non-destructive supersonic induced-luminessing imaging method and the device thereof that carries out the not damaged structure imaging of this endogenous.
Background technology
At present in the imaging technique that in medical imaging diagnosis, generally uses, X ray transmission imaging, x-ray tomography instrument (CT), Magnetic resonance imaging etc. all have largely damage to tissue, and the cost of x-ray tomography instrument, NMR imaging instrument is relatively more expensive; B-mode ultrasonography does not have damage substantially to human body, and the cost of B-mode ultrasonography instrument is also lower, but owing to be organizational interface's catoptric imaging, so can't realize chromatography, and resolution lower (to the in-vivo tissue of simple structure, best resolution is several millimeters), contrast is relatively poor.Optical image technology (comprises time discrimination optics imaging, the domain optical imaging, optical coherence tomography etc.) have the human zero damage, characteristics such as resolution height, but, because these methods all are to utilize external light source, and there are strong scattering and absorption in biological tissue to external source light, present technical merit can only reach the shallow-layer biological tissue imaging of the degree of depth for the millimeter magnitude, be difficult to the accurate measurement of realization to deep tissues, simultaneously, these method imagings all utilize optical properties of tissue to realize, its image contrast derives from optical properties of tissue difference only singlely.
Summary of the invention
The object of the present invention is to provide a kind of non-destructive supersonic induced-luminessing imaging method, utilization is luminous from the body endogenous to biosome harmless weak supersonic induced biosome or tissue generation, and utilizes this endogenous luminous with no damage to biosome or institutional framework realization accurately image.
Another object of the present invention is to provide a kind of device of implementing described non-destructive supersonic induced-luminessing imaging method.
Non-destructive supersonic induced-luminessing imaging method of the present invention comprises the steps:
---frequency is that the ultrasonic of 20KHz-10MHz directly acts on biosome or tissue, and induces biosome or tissue to produce luminous from the body endogenous;
---optical fiber receive module is electric signal with this from body endogenous luminescent conversion;
---electric signal is a digital signal by analog to digital conversion;
---processing digital signal is also carried out image reconstruction, obtains the structural images of biosome or tissue.
Ultrasonicly can continuous sound-field can be, also the pulse sound field can be by general sine signal source through power amplification rear drive ultrasonic transducer generating; Equally distributed sound field can be, also focusing acoustic field can be.Optical fiber receive module is the device that is used to survey light, for example CCD take pictures system, light confocal scanning system.Analog to digital conversion can realize with general analog to digital converter, the CCD controller that for example image series capture card of National Instruments company production, or Princeton Instruments company is produced.Image reconstruction can for example be realized with the LabVIEW software of National Instruments company exploitation or the WINVIEW software of Princeton Instruments company exploitation with general image treatment software in conjunction with computer realization.
When the used ultrasonic frequency of the present invention is 20KHz-10MHz, can obtain biosome or tissue image clearly; When ultrasonic frequency was 20KHz～1MHz, the image that obtains was the most clear, and signal to noise ratio (S/N ratio) can reach 3.47.
Non-destructive supersonic induced-luminessing imaging device of the present invention is made up of ultrasonic generating assembly, optical fiber receive module, analog to digital converter, computing machine, and ultrasonic generating assembly and computing machine are electrically connected, and optical fiber receive module, analog to digital converter and computing machine are electrically connected successively.
Ultrasonic generating assembly can adopt existing ultrasonic generating assembly, is electrically connected by function generator, power amplifier and ultrasonic transducer and forms.The invention people for specialized designs of the present invention a kind of ultrasonic transducer that more helps directly acting on biosome or tissue and form large tracts of land cylindricality sound field, so that obtain more distinct image, it is that a diameter is 50mm, the piezoelectric ceramic piece of thick 3mm, two discs are coated with silver conductive film, draw by lead, form two electrodes, it can be driven by the sinusoidal signal after amplifying through power amplifier and produce ultrasonic.A disc of ultrasonic transducer is fixed on the recirculated water box with heat sink glue, but ON cycle water makes transducer refrigeration during work.
Optical fiber receive module is the device that is used to survey light, for example CCD take pictures system, light confocal scanning system.
Analog to digital converter can adopt general analog to digital converter, or has other devices of analog-digital conversion function, the CCD controller that for example image series capture card of National Instruments company production, or Princeton Instruments company is produced.
General image treatment software is housed in the computing machine, for example, is used for biosome or tissue image and rebuilds and handle with the LabVIEW software of National Instruments company exploitation or the WINVIEW software of Princeton Instruments company exploitation.
Compared with prior art, the present invention has following advantage:
(1) this non-destructive supersonic induced-luminessing imaging method combines and ultrasonic body is had stronger penetration capacity and optical imagery has high-resolution, undamaged advantage, can not produce radioactive damage to biosome, thus compare with traditional medical imaging diagnosis method have not damaged, advantage that resolution is high;
(2) because the contrast of this non-destructive supersonic induced-luminessing imaging method derives from the characteristic that the acousta induction leaded light of tissue and sound field interaction property, tissue and light field interaction property and tissue produces, so compare with the single contrast source of traditional formation method, the contrast of this formation method has significant improvement;
(3) cost of each assembly of this supersonic induced not damaged imaging device is lower, so the cost of single unit system is also relatively low, again because the structure of this device is comparatively simple, so the production assembling is more or less freely, uses operation also more convenient.
Description of drawings
Fig. 1 is the block diagram of non-destructive supersonic induced-luminessing imaging device of the present invention;
Fig. 2 is fresh pig liver tissue (20 * 20 * 2mm) the outside drawings under the faint light photograph that exsomatize;
Fig. 3 is that the pig liver tissue of Fig. 2 is by weak supersonic induced luminescent image;
Fig. 4 is the outside drawing of a nude mice under faint light is shone;
Fig. 5 is that the nude mice of Fig. 4 is by weak supersonic induced luminescent image.
Embodiment
Fig. 1 shows the structure of non-destructive supersonic induced-luminessing imaging device of the present invention, as seen from Figure 1, this device is made up of ultrasonic generating assembly 1, optical fiber receive module 2, analog to digital converter 3, computing machine 4, and wherein ultrasonic generating assembly 1 is electrically connected by function generator 1-1, power amplifier 1-2, ultrasonic transducer 1-3 preface successively and constitutes; Optical fiber receive module 2 is connected and composed by take pictures camera lens 2-1 and detector 2-2; Ultrasonic generating assembly 1 is electrically connected with computing machine 4, and optical fiber receive module 2, analog to digital converter 3 are electrically connected successively with computing machine 4; 5 is that tested biosome (or tissue), 6 is the darkroom.Select for use each member to connect to form this device, wherein: the AFG320 type arbitrary-function generator that function generator 1-1 selects for use Tektronix company to make; The 2100L molded breadth band power amplifier that power amplifier 1-2 selects for use ENI company to make; Ultrasonic transducer 1-3 is that a diameter is 50mm, the piezoelectric ceramic piece of thick 3mm, and two discs are coated with silver conductive film, draw by lead, form two electrodes, and it can be driven by the sinusoidal signal after amplifying through power amplifier and produce ultrasonic.A disc of ultrasonic transducer is fixed on the recirculated water box with heat sink glue, but during work ON cycle water with the refrigeration transducer; The camera lens 2-1 that takes pictures selects the big digital aperture camera lens (50mm, f/1.2 type) of the Nikon company manufacturing that is applicable to that the low light level is taken for use; The refrigeration TE/CCD-512TKB type CCD of illumination dorsad that detector 2-2 selects for use U.S. PrincetonInstruments company to make; The ST-130 type controller that analog to digital converter 3 selects for use U.S. Princeton Instruments company to make; Computing machine 4 is selected the IBM586 microcomputer for use.
Said apparatus is applied to imaging to the biological tissue that exsomatizes, gets the fresh pig liver tissue that exsomatizes, be cut into one of 20 * 20 * 2mm size, be placed in the darkroom 6 on the ultrasonic transducer 1-3 faces, faint light according under write down as shown in Figure 2 outside drawing; Shut the darkroom, producing frequency by the computer regulated function generator is the sinusoidal signal of 1.2MHz, is 100W rear drive ultrasonic transducer 1-3 through power amplification, and ultrasonic a little less than producing, the power density of this weak ultrasonic arrival liver organization is about 0.38W/cm
2(ultrasonic to human body safety standard 23bar far below U.S. FDA is equivalent to 19.32W/cm
2), under weak ultrasonic inducing action, liver organization produces luminous from the body endogenous, detector 2-2 writes down luminous signal 15min and luminous signal is converted into electric signal by the camera lens 2-1 that takes pictures, 3 pairs of electric signal of analog to digital converter carry out analog to digital conversion be after the digital signal with its input computing machine, in computing machine, utilize the WINVIEW software of Princeton Instruments company exploitation to carry out image reconstruction and data processing and can obtain as shown in Figure 3 luminescent image.As seen from the figure, the inventive method can be to biological tissue's imaging, and image is more clear, and signal to noise ratio (S/N ratio) is 2.51.
Device described in the embodiment 1 is applied to imaging to biological living, gets experiment, put into after the anesthesia on the ultrasonic transducer 1-3 in darkroom 6 with one of nude mice, faint light according under record outside drawing as shown in Figure 4, shut the darkroom; Sinusoidal signal by computing machine 4 adjustment function generator 1-1 generation frequency 40KHz is enlarged into 50W rear drive ultrasonic transducer 1-3 through power amplifier 1-2, and is ultrasonic a little less than producing, and power density of this weak ultrasonic arrival mouse body is about 0.18W/cm
2(ultrasonic to human body safety standard 23bar far below U.S. FDA is equivalent to 19.32W/cm
2), under weak ultrasonic inducing action, nude mouse produces luminous from the body endogenous, detector 2-2 writes down luminous signal 15min and luminous signal is converted into electric signal by the camera lens 2-1 that takes pictures, it is with its input computing machine 4 after the digital signal that 3 pairs of electric signal of analog to digital converter carry out analog to digital conversion, in computing machine 4, utilize the WINVIEW software of Princeton Instruments company exploitation to carry out image reconstruction and data processing and can obtain as shown in Figure 5 luminescent image, visual picture is clear, and signal to noise ratio (S/N ratio) reaches 3.47.The inventive method is described preferably to the biosome imaging, also explanation is when described ultrasonic frequency is 20KHz～1MHz, and the image that obtains is the most clear.
Claims (4)
1, a kind of non-destructive supersonic induced-luminessing imaging method comprises the steps:
---frequency is that the ultrasonic of 20KHz-10MHz directly acts on biosome or tissue, and induces biosome or tissue to produce luminous from the body endogenous;
---optical fiber receive module is electric signal with this from body endogenous luminescent conversion;
---electric signal is a digital signal by analog to digital conversion;
---processing digital signal is also carried out image reconstruction, obtains the structural images of biosome or tissue.
2,, it is characterized in that described ultrasonic frequency is 20KHz-1MHz according to the described non-destructive supersonic induced-luminessing imaging method of claim 1.
3, a kind of device of implementing non-destructive supersonic induced-luminessing imaging method as claimed in claim 1, form by ultrasonic generating assembly, optical fiber receive module, analog to digital converter, computing machine, ultrasonic generating assembly and computing machine are electrically connected, and optical fiber receive module, analog to digital converter and computing machine are electrically connected successively; Ultrasonic generating assembly is electrically connected by function generator, power amplifier and ultrasonic transducer to be formed.
4, according to the described device of claim 3, the ultrasonic transducer that it is characterized in that constituting ultrasonic generating assembly is that a diameter is 50mm, the piezoelectric ceramic piece of thick 3mm, two discs are coated with silver conductive film, draw by lead, form two electrodes, one of them disc is fixed on the recirculated water box with heat sink glue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001172093A CN1145793C (en) | 2000-06-27 | 2000-06-27 | Non-destructive supersonic induced-luminessing imaging method and equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001172093A CN1145793C (en) | 2000-06-27 | 2000-06-27 | Non-destructive supersonic induced-luminessing imaging method and equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1274845A CN1274845A (en) | 2000-11-29 |
| CN1145793C true CN1145793C (en) | 2004-04-14 |
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| CNB001172093A Expired - Fee Related CN1145793C (en) | 2000-06-27 | 2000-06-27 | Non-destructive supersonic induced-luminessing imaging method and equipment |
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