CN114574430A - Expi293F cell culture method - Google Patents
Expi293F cell culture method Download PDFInfo
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- 238000004113 cell culture Methods 0.000 title description 9
- 239000000243 solution Substances 0.000 claims abstract description 64
- 238000003756 stirring Methods 0.000 claims abstract description 36
- 230000010412 perfusion Effects 0.000 claims abstract description 35
- 238000002347 injection Methods 0.000 claims abstract description 25
- 239000007924 injection Substances 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 15
- 230000008569 process Effects 0.000 claims abstract description 12
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 6
- 241001212789 Dynamis Species 0.000 claims abstract description 5
- 230000001464 adherent effect Effects 0.000 claims abstract description 5
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims abstract description 5
- 230000010261 cell growth Effects 0.000 claims description 38
- 230000012010 growth Effects 0.000 claims description 9
- 238000012136 culture method Methods 0.000 claims description 7
- 230000005526 G1 to G0 transition Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 238000012546 transfer Methods 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 6
- 230000006378 damage Effects 0.000 abstract description 5
- 230000019522 cellular metabolic process Effects 0.000 abstract description 4
- 239000001963 growth medium Substances 0.000 abstract description 4
- 239000002609 medium Substances 0.000 abstract description 4
- 230000009467 reduction Effects 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 43
- 230000000052 comparative effect Effects 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 210000004102 animal cell Anatomy 0.000 description 2
- 238000012007 large scale cell culture Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N5/0684—Cells of the urinary tract or kidneys
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- C12N2500/32—Amino acids
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- C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
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- C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
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Abstract
The invention discloses a method for culturing Expi293F cells, which comprises the steps of inoculating Expi293F cells to a bioreactor for culture, and performing perfusion dynamic culture for 32-48 h: equally dividing the culture solution to be perfused into a plurality of strands, perfusing the culture solution into the bioreactor along the inner wall of the bioreactor adherent thereto, wherein the stirring speed in the bioreactor is 80-90 rpm, the perfusion rate of the perfusion solution is 0.5-2 working volumes/d, and when perfusing dynamic culture, maintaining the culture conditions in the reactor: the temperature is 36.5-37 ℃, DO is 40-60%, and the pH value is 7.2 +/-0.2; the serum-free culture solution used in the culture process is as follows: 24.75g/L Dynamis medium 95wt%, 200 mM glutamine 4wt% and anti-caking agent 1 wt%. The invention improves the perfusion mode, and then, by matching with proper perfusion rate and reduction of stirring strength, the injection of the culture medium in the perfusion process also plays roles in expanding mass transfer, reducing the influence of cell clusters on cell metabolism and reducing physical damage to cells, and achieves remarkable effects of high-density cells and high survival rate.
Description
Technical Field
The invention relates to the field of cell culture, and in particular relates to an Expi293F cell culture method.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Large scale cell culture is often performed using bioreactors. A bioreactor is a container for biologically converting raw materials into products using microorganisms, plants, animals or human cells.
The cell culture bioreactor can be used for large-scale cell culture, and has the advantages of automatic control and real-time monitoring of culture conditions in the cell culture process, and relatively stable adjustment of the whole production conditions, so that the stability of product quality can be improved. However, in the actual production process, when the volume of the reactor is increased, in order to maintain the uniformity of the environment, the intensity of stirring needs to be increased, but thus higher shear rate and shear force are brought to physically damage the cells; however, if the stirring intensity is reduced, mass transfer is not facilitated.
Disclosure of Invention
In order to solve the problems, the invention provides an Expi293F cell culture method, so that the injection of a culture medium in a perfusion process also plays a role in expanding mass transfer and reducing cell aggregation, and high-density cells are obtained.
The invention is realized by the following technical scheme:
a culture method of Expi293F cells comprises inoculating Expi293F cells into bioreactor for culture, and perfusing
Culturing for 32-72 h: equally dividing a culture solution to be perfused into a plurality of strands, perfusing the culture solution into a bioreactor along the inner wall of the bioreactor adherent thereto, wherein the stirring speed in the bioreactor is 80-90 rpm, the perfusion rate of the perfusion solution is 0.5-2 working volumes/d, the stirring speed in the cell growth vigorous phase is lower than the stirring speed in the cell growth initial phase and the cell growth stable phase, the perfusion speed in the cell growth vigorous phase is higher than the perfusion speed in the cell growth initial phase and the cell growth stable phase, and when perfusing dynamic culture, maintaining the culture conditions in the reactor: the temperature is 36.5-37 ℃, DO is 40-60%, and the pH value is 7.2 +/-0.2; the serum-free culture solution used in the culture process is as follows: 24.75g/L Dynamis medium 95wt%, 200 mM glutamine 4wt% and anti-caking agent 1 wt%.
During perfusion culture, the culture solution to be perfused is equally divided into a plurality of strands and is uniformly perfused into the reactor along the wall of the reactor. The split design can be made according to the specific volume of the bioreactor. The dead angle of stirring often occurs at the inner edge of the reactor far from the stirrer, and although the stirrer is improved in the prior art to solve the problem, the stirrer still has a position where the stirring is not in place more or less, and thus the cell growth is affected due to the uneven stirring, thereby affecting the heat balance. The culture solution that injects into when a plurality of routes can and because of the stirring effect raise the culture solution that is close to the reactor inner wall and carry out the upper and lower mixed mass transfer of many extents, lets cell metabolism fully go on, reduces the mechanical damage to animal cells when realizing even mass transfer, improves animal cells's activity and quality, can realize the purpose that obtains high-density cell fast at last. The invention improves the perfusion mode, and then, by matching with proper perfusion rate and reduction of stirring strength, the injection of the culture medium in the perfusion process also plays roles in expanding mass transfer, reducing influence of cell clusters on cell metabolism and reducing physical damage to cells, obtains high-density cells and obtains remarkable effect.
In the process of perfusion dynamic culture conditions, in the initial stage of cell growth, the injection rate of a culture solution is 0.5 working volume/d, and the stirring speed is 85-86 rpm; in the stationary phase of cell growth, the injection speed of the culture solution is 1 working volume/d, and the stirring speed is 84-85 rpm. The temperature of the perfused culture solution and the temperature of the culture solution in the reactor are set to be equal in the early growth stage and the final growth stage of the cells, namely the temperature of the perfusate is consistent with the temperature controlled by the reactor.
In the process of perfusion dynamic culture conditions, the growth of cells is in a vigorous stage, the injection rate of the culture solution is 1.5-2 working volumes/d, and the stirring speed is 80-83 rpm. The injection speed and the stirring speed of different growth periods are matched to maintain relatively stable environment heat in the cell culture process, so that the environment requirements of the cells in different growth periods and the requirements of culture quality are met.
In the period of vigorous cell growth, the temperature of perfused culture solution is 0.1-0.4 deg.C lower than that of the culture solution in the reactor, i.e. the temperature of perfusate is 0.1-0.4 deg.C lower than that of the reactor. In the vigorous cell growth stage, the temperature of the perfusion solution is reduced by 0.1-0.4 ℃, and the cell density is further improved. This may be that when the Expi293F cells grow in exponential phase, the metabolism is very vigorous, the heat generated by the metabolism cannot be timely evacuated to the reaction environment, a local temperature gradient appears in the reaction solution, and the local temperature gradient cannot be well controlled due to the lag of the temperature control system and the detection limitation, and the sudden change of the local heat will affect the growth of the cells, so the temperature of the perfusion solution is slightly lowered by the present invention, the fluctuation frequency of the heat in the reactor is reduced, the reaction heat in the reactor is relatively balanced and stable, the influence of the temperature gradient on the growth of the cells is reduced, and the cell density is increased. In practice it has been found that a reduction of 0.1-0.2 ℃ is more favourable for the balance of heat in the environment in which the cells are growing, i.e. for the balance of the growing environment.
The cell transferred to the bioreactor to be cultured has the inoculation density of 0.2 multiplied by 106cells/ml. A plurality of feed holes are arranged in the reactor along the inner wall of the reactor, the perfusate enters the reactor through the feed holes, and the lower ends of the feed holes are arranged at the half position of the height of the culture solution in the reactor. The anticaking agent is commonly used, such as F68.
The feed port is connected with a stainless steel short pipe with the length of 1-2cm, the purpose is to prevent the perfusate from directly entering and influencing the cells too much, the short pipe can play a role in temporarily isolating the perfusate and the cells, and the temperature influence on the cells when the perfusate is in direct contact with the cells can be reduced after the perfusate and the short pipe exchange heat.
The utility model discloses a reactor, including reactor, reaction vessel, the reactor is equipped with the continuous flowing of nutrient solution, the continuous flowing of nutrient solution of many degrees of many ranges is compared the injection of single strand nutrient solution in the reactor, the reaction vessel is equipped with the omnidirectional dissolved oxygen of liquid and is gone on more, make the cell be in a dynamic stable better reproductive environment in a longer time, can realize reducing the stirring rate and reduce the cell damage, but can obtain the effect of high density cell again. Expi293F cells were purchased from ATCC.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. the invention can obtain the cell density of Expi293F as high as 0.9-1.3X 108cells/ml。
2. The invention improves the perfusion mode, and then, by matching with proper perfusion rate and reduction of stirring strength, the injection of the culture medium in the perfusion process also plays roles in expanding mass transfer, reducing the influence of cell clusters on cell metabolism and reducing physical damage to cells, and achieves remarkable effects of high-density cells and high survival rate.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not used as limitations of the present invention.
EXAMPLE 1 culture of Expi293F cells
According to a cell density of 0.2X 106cells/ml are inoculated into a 50L Qizhi bioreactor (working volume is 40L) for perfusion dynamic culture for 48h, the serum-free culture solution to be perfused is equally divided into 6 strands, (the culture solution to be perfused is preheated to 37 ℃), the culture solution is uniformly dispersed along the inner wall of the bioreactor, the cells are perfused into the bioreactor adherent to walls, and the culture conditions in the bioreactor are maintained during perfusion dynamic culture: the temperature is 37 ℃, DO is 40-60%, and the pH value is 7.2 +/-0.2; the serum-free culture solution used in the culture process is as follows: 24.75g/L Dynamis medium 95wt%, 200 mM glutamine 4wt%, anti-caking agent 1 wt%.
The culture solution injection rate was: in the initial stage of cell growth, the injection rate of the culture solution is 0.5 working volume/d, the stirring speed is 85 rpm, the cell growth is in a stable period, the injection rate of the culture solution is 1 working volume/d, the stirring speed is 84 rpm, the cell growth is in a vigorous period, the injection rate of the culture solution is 1.5 working volume/d, and the stirring speed is 83 rpm.
Example 2
According to a cell density of 0.2X 106cells/ml are inoculated into a 50-liter bioreactor for perfusion dynamic culture for 48 hours, the culture solution to be perfused is equally divided into 6 strands, (the culture solution to be perfused is preheated to 37 ℃), the culture solution is uniformly dispersed along the inner wall of the bioreactor, the culture solution is perfused into the bioreactor adherent to the wall, and the culture conditions in the bioreactor are maintained during perfusion dynamic culture: the temperature is 37 ℃, DO is 40-60%, and the pH value is 7.2 +/-0.2; the serum-free culture solution used in the culture process is as follows: 24.75g/L Dynamis medium 95wt%, 200 mM glutamine 4wt% and anti-caking agent 1 wt%.
The culture solution injection rate was: in the initial stage of cell growth, the injection rate of the culture solution is 0.5 working volume/d, the stirring speed is 86 rpm, the cell growth is in a stable period, the injection rate of the culture solution is 1 working volume/d, the stirring speed is 85 rpm, the cell growth is in a vigorous period, the injection rate of the culture solution is 2 working volumes/d, and the stirring speed is 81 rpm.
Example 3
Similar to example 2, except that the temperature of the culture solution to be perfused was 0.1 ℃ lower than that of the culture solution in the reactor after the pre-heating treatment of the culture solution during the cell growth phase.
Example 4
Similar to example 2, except that the temperature of the perfused culture broth was 0.2 ℃ lower than that of the culture broth in the reactor during the cell growth phase.
Example 5
Similar to example 2, except that the temperature of the perfused culture broth was 0.3 ℃ lower than that of the culture broth in the reactor during the cell growth phase.
Example 6
Similar to example 2, except that the temperature of the perfused culture broth was 0.4 ℃ lower than that of the culture broth in the reactor during the cell growth phase.
Comparative example 1
Similar to example 2, except that the temperature of the perfused culture broth was 0.6 ℃ lower than that of the culture broth in the reactor during the cell growth phase.
Comparative example 2
Similar to example 2, except that the temperature of the perfused culture broth was 0.7 ℃ lower than that of the culture broth in the reactor during the cell growth phase.
Comparative example 3
Similar to example 2, except that the temperature of the perfused culture broth was 0.5 ℃ lower than that of the culture broth in the reactor during the cell growth phase.
Comparative example 4
Similar to example 2, except that no split flow was used for perfusion.
Comparative example 5
Similar to example 2, except that the injection rate of the culture solution was 2 working volumes/d, the stirring speed was 86 rpm, the cell growth stationary phase was 1 working volume/d, the stirring speed was 85 rpm, the cell growth vigorous phase was 0.5 working volume/d, and the stirring speed was 81 rpm at the initial stage of cell growth.
Comparative example 6
Similar to example 2, except that the culture solution injection rate was: in the initial stage of cell growth, the injection rate of the culture solution is 0.5 working volume/d, the stirring speed is 85 rpm, the cell growth is in a stable period, the injection rate of the culture solution is 1 working volume/d, the stirring speed is 84 rpm, the cell growth is in a vigorous period, the injection rate of the culture solution is 2 working volumes/d, and the stirring speed is 90 rpm.
In carrying out the above in the examples and comparative examples, it is possible to carry out the following as required: set up 6 feed ports along its inner wall in the reactor, the perfusate is equallyd divide through 6 feed ports and gets into in the reactor, and the lower extreme setting of feed port is in the reactor half position of culture solution height department. The feeding hole is connected with a stainless steel short pipe with the length of 1-2 cm.
Expi293F cells were produced according to the production process described in the examples and comparative examples, and the cell density of Expi293F cells after culture was measured and compared with the cell density results of 3 batches after culture using the static culture process, the results are shown in Table 1 below:
as can be seen from the examples and comparative examples, the present invention can obtain high density cells and effectively improve the survival rate of the cells, and for fine adjustment of the temperature of the perfusate, it can effectively balance the heat of the cells generated by rapid growth of the cells in the culture solution with the heat of the environment, and reduce the influence of the step heat which is not balanced in the culture solution on the cells, so that examples 3 and 4 show superior results, while too much difference in the culture temperature affects the stability of the culture environment, and thus examples 5 and 6 show inferior results, while the results of comparative examples 1 to 3 are less desirable.
In the present invention, it is not described in detail in the prior art.
The above-mentioned embodiments, objects, technical solutions and advantages of the present invention are further described in detail, it should be understood that the above-mentioned embodiments are only examples of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (7)
1. A culture method of Expi293F cells is characterized in that Expi293F cells are inoculated into a bioreactor for culture, and perfusion dynamic culture is adopted for 32-72 h: equally dividing a culture solution to be perfused into a plurality of strands, perfusing the culture solution into a bioreactor along the inner wall of the bioreactor adherent thereto, wherein the stirring speed in the bioreactor is 80-90 rpm, the perfusion rate of the perfusion solution is 0.5-2 working volumes/d, the stirring speed in the cell growth vigorous phase is lower than the stirring speed in the cell growth initial phase and the cell growth stable phase, the perfusion speed in the cell growth vigorous phase is higher than the perfusion speed in the cell growth initial phase and the cell growth stable phase, and when perfusing dynamic culture, maintaining the culture conditions in the reactor: the temperature is 36.5-37 ℃, DO is 40-60%, and the pH value is 7.2 +/-0.2; the serum-free culture solution used in the culture process is as follows: 24.75g/L Dynamis medium 95wt%, 200 mM glutamine 4wt% and anti-caking agent 1 wt%.
2. The culture method of claim 1, wherein the cells are cultured during perfusion of the cells during dynamic culture conditions
In the initial growth stage, the injection rate of the culture solution is 0.5 working volume/d, and the stirring speed is 85-86 rpm; in the stationary phase of cell growth, the injection speed of the culture solution is 1 working volume/d, and the stirring speed is 84-85 rpm.
3. The culture method of claim 1, wherein the cells are cultured during perfusion of the cells during dynamic culture conditions
In the vigorous growth stage, the injection rate of the culture solution is 1.5-2 working volumes/d, and the stirring speed is 80-83 rpm.
4. The culture method according to claim 3, wherein the temperature of the perfused culture solution is 0.1 to 0.4 ℃ lower than the temperature of the culture solution in the reactor.
5. The method of claim 1, wherein the cells transferred to the bioreactor are inoculated
Density of 0.2X 106 cells/ml。
6. The culture method according to claim 1, wherein a plurality of feed holes are provided in the bioreactor along an inner wall thereof, the perfusion liquid is introduced into the reactor through the feed holes, and lower ends of the feed holes are provided at a position half the height of the culture liquid in the reactor.
7. The culture method according to claim 6, wherein the inlet port is connected to a stainless steel short tube having a length of 1 to 2 cm.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140255993A1 (en) * | 2011-07-01 | 2014-09-11 | Amgen Inc. | Mammalian cell culture |
| US20200270583A1 (en) * | 2019-02-22 | 2020-08-27 | Life Technologies Corporation | Suspension system for adeno associated virus protection |
| GB202019455D0 (en) * | 2020-12-10 | 2021-01-27 | Chancellor Masters And Scholars Of The Univ Of Oxford | Method for producing virus |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140255993A1 (en) * | 2011-07-01 | 2014-09-11 | Amgen Inc. | Mammalian cell culture |
| US20200270583A1 (en) * | 2019-02-22 | 2020-08-27 | Life Technologies Corporation | Suspension system for adeno associated virus protection |
| GB202019455D0 (en) * | 2020-12-10 | 2021-01-27 | Chancellor Masters And Scholars Of The Univ Of Oxford | Method for producing virus |
Non-Patent Citations (1)
| Title |
|---|
| LÁZARO-IBÁÑEZ E, 等: "Selection of Fluorescent, Bioluminescent, and Radioactive Tracers to Accurately Reflect Extracellular Vesicle Biodistribution in Vivo", 《ACS NANO.》, pages 3212 - 3227 * |
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