CN114574333B - Nerve cell culture dish - Google Patents
Nerve cell culture dish Download PDFInfo
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- CN114574333B CN114574333B CN202210170822.9A CN202210170822A CN114574333B CN 114574333 B CN114574333 B CN 114574333B CN 202210170822 A CN202210170822 A CN 202210170822A CN 114574333 B CN114574333 B CN 114574333B
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- grid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
Abstract
The invention discloses a nerve cell culture dish, which comprises: the culture plates are of a grid structure, culture medium liquid can form a liquid film on the grid structure, and the liquid film can bear nerve cells to be cultured; the moving structure is connected with the culture plates and can drive the culture plates to move towards a direction away from each other and a direction close to each other; the culture dish is provided with a plurality of culture plates and a moving structure, a plurality of culture plates are arranged to form multi-layer culture, the moving structure can enable the culture plates bearing liquid films and nerve cells to be cultured to be close to each other, myelin sheaths and axons grow on the nerve cells along with the culture between the nerve cells on two adjacent layers of culture plates, synaptic connections between the axons in the upper direction and the lower direction can be formed between the axons of two adjacent layers of nerve cells, and the actual conditions of the cells in a body are reflected more truly.
Description
Technical Field
The invention belongs to the technical field of nerve cell culture, and particularly relates to a nerve cell culture dish.
Background
The culture of nerve cells in vitro has become a very useful technical means in neurobiological research. The main advantages of neural cell culture are: (1) After the neural cells in the dispersed culture grow and mature in vitro, certain characteristics in structure and function can be maintained, and myelination and synaptic connection establishment can be realized by long-term culture, so that the opportunity of reproducing the in-vivo growth process in vitro is provided; (2) The growth, differentiation, morphological and functional changes of living cells can be directly observed in a long time, and the research can be conveniently carried out by using various technical methods such as a phase contrast microscope, a fluorescence microscope, an electron microscope, a laser confocal microscope, isotope labeling, in-situ hybridization, immunohistochemistry, electrophysiology and other means; (3) The experimental conditions such as physical (such as ischemia and anoxia), chemical and biological factors (such as neurotrophic factors) and the like are easy to implement, and the direct or indirect effect of the condition change on nerve cells is observed; (4) It is convenient to study the pathogenesis of some nerve diseases from the cellular and molecular level, and the influence of drugs or various factors on the growth, development, differentiation and other aspects of the nerve cells of the embryo or the newborn animal.
However, the conventional culture dish is planar, and cultured nerve cells grow in a flat manner in front, back, left and right directions, while nerve cell lines in a real organism grow in a layered overlapping manner, so that the conventional culture dish cannot truly reflect the actual conditions of cells in the organism.
Disclosure of Invention
The invention aims to provide a nerve cell culture dish which is provided with a plurality of culture plates and a moving structure, wherein a multi-layer culture is formed by arranging the culture plates, the culture plates carrying liquid films and nerve cells to be cultured can be close to each other by the moving structure, myelin sheaths and axons grow on the nerve cells along with the culture, and synaptic connections between the axons in the vertical direction can be formed between the axons of the nerve cells in two adjacent layers, so that the actual conditions of the cells in a body can be reflected more truly.
In order to achieve the above object, the present invention provides a neural cell culture dish, including:
the culture plates are of a grid-shaped structure, and a culture medium liquid can form a liquid film on the grid-shaped structure, and the liquid film can bear the nerve cells to be cultured;
the moving structure is connected with the culture plates and can drive the culture plates to move towards the direction away from each other and towards the direction close to each other.
Optionally, the material of the culture plate comprises a mixture of gelatin, sodium alginate, calcium chloride.
Optionally, the grid-like structure includes a plurality of transverse partition walls and a plurality of longitudinal partition walls, and the plurality of transverse partition walls and the plurality of longitudinal partition walls are arranged in a staggered manner to form a plurality of grids.
Optionally, the grid is a square with a side length of 1 mm.
Optionally, a connecting hole is arranged on the transverse partition wall and/or the longitudinal partition wall between the adjacent grids, and the connecting hole is used for communicating the adjacent grids.
Optionally, a frame is provided on the periphery of each of the culture plates.
Optionally, the frame is rectangular or circular.
Optionally, the thickness of the bezel is greater than the thickness of the grid-like structure.
Optionally, the moving structure includes a V-shaped structure formed by two connecting rods, and two ends of the V-shaped structure are respectively hinged to two adjacent frames.
Optionally, when two adjacent frames are stacked, a gap of 0.1-0.5mm is formed between two adjacent layers of grid-shaped structures.
The invention provides a nerve cell culture dish, which has the beneficial effects that:
1. the culture dish is provided with a plurality of culture plates and a moving structure, a plurality of culture plates are arranged to form multi-layer culture, the moving structure can enable the culture plates which bear liquid films and nerve cells to be cultured to be close to each other, myelin sheaths and axons grow on the nerve cells along with the culture between the nerve cells on two adjacent layers of culture plates, and synaptic connections between the axons in the upper direction and the lower direction can be formed between the axons of two adjacent layers of nerve cells, so that the actual conditions of the cells in a body can be reflected more truly;
2. the connecting holes are formed in the transverse partition wall and/or the longitudinal partition wall between the adjacent grids of the culture dish, so that synaptic connection between axons in the growth process of nerve cells in the adjacent grids can be realized.
Additional features and advantages of the invention will be set forth in the detailed description which follows.
Drawings
The above and other objects, features and advantages of the present invention will become more apparent by describing in detail exemplary embodiments thereof with reference to the attached drawings, wherein like reference numerals generally represent like parts in the exemplary embodiments of the present invention.
Fig. 1 is a schematic front view showing a structure of a nerve cell culture dish according to an embodiment of the present invention.
Fig. 2 is a schematic diagram showing a top view of a culture plate of a nerve cell culture dish according to an embodiment of the present invention.
Description of the reference numerals:
1. culturing the plate; 2. a moving structure; 3. a transverse partition wall; 4. a longitudinal partition wall; 5. a grid; 6. connecting holes; 7. a frame; 8. a V-shaped structure.
Detailed Description
Preferred embodiments of the present invention will be described in more detail below. While the following describes preferred embodiments of the present invention, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
The present invention provides a nerve cell culture dish, comprising:
the culture plates are of a grid structure, culture medium liquid can form a liquid film on the grid structure, and the liquid film can bear nerve cells to be cultured;
the moving structure is connected with the culture plates and can drive the culture plates to move towards the direction away from each other and towards the direction close to each other.
Specifically, under the action of the moving structure, the plurality of culture plates can be separated from each other, so that a culture medium liquid is conveniently added to each culture plate, the grid-shaped structure is covered by the culture medium liquid under the action of surface tension to form a liquid film, and the neural cells to be cultured can be cultured by putting the neural cells to be cultured on the liquid film; the two adjacent culture plates are close to each other through the moving structure before the nerve cells to be cultured are cultured, the gap between the two adjacent culture plates is well controlled, the nerve cells to be cultured on the two adjacent culture plates can grow and form connection between the two adjacent culture plates in the culture process, the layered overlapped growth of the nerve cells which better accord with the real situation can be realized, and the actual growth condition of the nerve cells in the organism can be reflected more really.
Optionally, the material of the culture plate comprises a mixture of gelatin, sodium alginate, calcium chloride.
Specifically, the latticed structure material is formed by mixing gelatin, sodium alginate and calcium chloride, and the manufacturing method comprises the steps of taking 4.0g of sodium alginate, and fully stirring the sodium alginate with 100ml of distilled water at 60 ℃ to obtain a sodium alginate solution I with the mass fraction of 4 percent; taking 8.0g of gelatin granules, fully stirring the gelatin granules with 100m1 of distilled water at 60 ℃ to obtain a gelatin solution with the mass fraction of 8% to form a solution II; 4.0g of calcium chloride is taken to be fully stirred with 100m1 of distilled water at 60 ℃ to obtain a calcium chloride solution with the mass fraction of 4 percent to form a solution III; according to the first solution: solution II: solution three is prepared by mixing the following components in a volume ratio of 2:1:1, mixing and stirring to form a solution IV; and (3) arranging a groove with the depth of about 1mm on the mould, pouring the solution IV on the mould immediately after the preparation is finished, then taking out the mould after the mould is rotated and refrigerated in a refrigerator for 10 minutes, taking out the solid grid-shaped structure formed by the solution IV from the mould, and punching 1-2 holes on the interval parts of adjacent grids.
Optionally, the grid-like structure comprises a plurality of transverse partition walls and a plurality of longitudinal partition walls, and the plurality of transverse partition walls and the plurality of longitudinal partition walls are arranged in a staggered manner to form a plurality of grids.
Optionally, the grid is a square with a side of 1 mm.
In particular, the size of the mesh facilitates the formation of a liquid film.
Optionally, the transverse partition walls and/or the longitudinal partition walls between the adjacent grids are provided with connecting holes, and the connecting holes are used for communicating the adjacent grids.
Specifically, the arrangement of the connecting holes can realize the connection in the growth process of the nerve cells in the adjacent grids.
Optionally, a frame is provided on the periphery of each plate.
Specifically, the frame can be dismantled with the culture plate and be connected, can cultivate the board more and form support and reinforcing action.
Optionally, the frame is rectangular or circular.
Optionally, the thickness of the bezel is greater than the thickness of the grid-like structure.
Specifically, frame thickness is greater than the thickness of latticed structure, forms the arch in latticed structure's both sides, when two cultivation boards stack, naturally forms the clearance between two cultivation boards when the frame of two cultivation boards contacts each other.
Optionally, the moving structure includes a V-shaped structure formed by two connecting rods, and two ends of the V-shaped structure are respectively hinged to two adjacent side frames.
Specifically, the hinged parts of the V-shaped structure and the hinged parts of the two ends of the V-shaped structure and the frame are hinged with damping.
Optionally, when two adjacent frames are stacked, a gap of 0.1-0.5mm is formed between two adjacent layers of grid-like structures.
Specifically, when two adjacent frames are stacked, the gaps between two adjacent layers of grid-shaped structures enable nerve cells on two layers of grids to grow vertically and form connection.
Examples
As shown in fig. 1 and 2, the present invention provides a neural cell culture dish, including:
the culture plates 1 are of a grid structure, culture medium liquid can form a liquid film on the grid structure, and the liquid film can bear nerve cells to be cultured;
the moving structure 2 is connected with the culture plates 1, and the moving structure 2 can drive the culture plates 1 to move towards the direction away from each other and towards the direction close to each other.
In this embodiment, the material of the culture plate 1 comprises a mixture of gelatin, sodium alginate, calcium chloride.
In the present embodiment, the grid-like structure includes a plurality of transverse partition walls 3 and a plurality of longitudinal partition walls 4, and the plurality of transverse partition walls 3 and the plurality of longitudinal partition walls 4 are alternately arranged to form a plurality of grids 5.
In the present embodiment, the grid 5 is a square having a side of 1 mm.
In the present embodiment, the transverse partition walls 3 and/or the longitudinal partition walls 4 between the adjacent grids 5 are provided with connection holes 6, and the connection holes 6 communicate the adjacent grids 5.
In the present embodiment, a frame 7 is provided on the outer periphery of each culture plate 1.
In the present embodiment, the frame 7 is rectangular or circular.
In this embodiment, the thickness of the frame 7 is greater than the thickness of the grid-like structure.
In the present embodiment, the moving structure 2 includes a V-shaped structure 8 formed by two connecting rods, and two ends of the V-shaped structure 8 are respectively hinged to two adjacent frames 7.
In the present embodiment, when two adjacent frames 7 are stacked, a gap of 0.5mm is formed between two adjacent layers of the grid-like structures.
In conclusion, when the nerve cell culture dish provided by the invention is used, two ends of the V-shaped structure 8 are separated by a certain distance, so that two adjacent culture plates 1 are separated by a certain distance, a culture medium liquid is conveniently added, the culture medium liquid covers the latticed structure due to the action of surface tension, and a liquid film is formed on the latticed structure; then adding the neural cells to be cultured, and distributing the neural cells in each grid 5; the two ends of the V-shaped structure 8 are combined again, so that the frames 7 of two adjacent culture plates 1 are contacted with each other to form a stack, and a tiny gap is formed between the liquid films in two adjacent layers of grids 5. Culturing the nerve cells to be cultured under a certain condition, and after a certain time, connecting the nerve cells in two adjacent grids in the same culture plate through the connecting holes 6, and connecting the nerve cells in two corresponding grids in two adjacent layers of culture plates 1; at this point, the frame 7 is removed again and the grid-like structure is formalin-fixed, dehydrated, embedded (wax block), sliced, baked, stained, mounted and read.
Having described embodiments of the present invention, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments.
Claims (7)
1. A nerve cell culture dish, comprising:
the culture plates are of a grid-shaped structure, and a culture medium liquid can form a liquid film on the grid-shaped structure, and the liquid film can bear nerve cells to be cultured;
the moving structure is connected with the culture plates and can drive the culture plates to move towards a direction away from each other and a direction close to each other;
a frame is arranged on the periphery of each culture plate; the thickness of the frame is larger than that of the grid-shaped structure; when two adjacent frames are stacked, a gap of 0.1-0.5mm is formed between two adjacent layers of grid-shaped structures.
2. The nerve cell culture dish of claim 1, wherein the material of the culture plate comprises a mixture of gelatin, sodium alginate, calcium chloride.
3. The nerve cell culture dish of claim 1, wherein the grid-like structure comprises a plurality of transverse partition walls and a plurality of longitudinal partition walls, and the plurality of transverse partition walls and the plurality of longitudinal partition walls are arranged in a staggered manner to form a plurality of grids.
4. A nerve cell culture dish according to claim 3, wherein the grid is a square with a side of 1 mm.
5. The nerve cell culture dish according to claim 3, wherein the transverse partition walls and/or the longitudinal partition walls between the adjacent grids are provided with connecting holes, and the connecting holes communicate the adjacent grids.
6. The nerve cell culture dish of claim 1, wherein the frame is rectangular or circular.
7. The nerve cell culture dish of claim 1, wherein the moving structure comprises a V-shaped structure formed by two connecting rods hinged together, and two ends of the V-shaped structure are hinged to two adjacent frames respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210170822.9A CN114574333B (en) | 2022-02-23 | 2022-02-23 | Nerve cell culture dish |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210170822.9A CN114574333B (en) | 2022-02-23 | 2022-02-23 | Nerve cell culture dish |
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| CN114574333A CN114574333A (en) | 2022-06-03 |
| CN114574333B true CN114574333B (en) | 2023-01-10 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2009150051A2 (en) * | 2008-05-27 | 2009-12-17 | Aarhus Universitet | Biocompatible materials for mammalian stem cell growth and differentiation |
| CN102533652B (en) * | 2010-12-11 | 2014-01-15 | 清华大学 | Preparation method for nerve transplant bodies |
| CN102156158B (en) * | 2010-12-28 | 2013-03-20 | 重庆大学 | Device for culturing and measuring microfluidic chip by using topological diagram type nerve cell network |
| CN102911912B (en) * | 2011-08-01 | 2014-12-31 | 清华大学 | Culture matrix and nerve graft by using same |
| CN102911913B (en) * | 2011-08-01 | 2015-03-11 | 清华大学 | Application method of culture matrix |
| CN103966095B (en) * | 2014-05-22 | 2016-01-20 | 中国人民解放军第三军医大学第三附属医院 | A kind of bio-reactor being applicable to three-dimensional tissue's Cell infusion and cultivating |
| CN208649286U (en) * | 2018-06-04 | 2019-03-26 | 昆明轿子山科技开发有限公司 | A kind of meat products fermenting frame |
| CN209508276U (en) * | 2018-09-06 | 2019-10-18 | 中国医学科学院阜外医院 | It is a kind of for cultivating the electrical stimulation device of cell |
| CN113015786A (en) * | 2018-11-14 | 2021-06-22 | 阿莫绿色技术有限公司 | Cell culture piece subassembly for large capacity incubator reaches large capacity incubator including it |
| CN111748443B (en) * | 2019-03-29 | 2023-11-17 | 北京大橡科技有限公司 | 3D multilayer high-flux organ chip and preparation method and application thereof |
| CN210656957U (en) * | 2019-05-08 | 2020-06-02 | 河北师范大学 | Plant cell culture device |
| CN113462566B (en) * | 2021-07-27 | 2023-04-28 | 北京理工大学 | Nerve bundle construction stent and nerve bundle construction method |
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