CN114561409B - Quinoa CqALS gene mutant and molecular identification method and application - Google Patents
Quinoa CqALS gene mutant and molecular identification method and application Download PDFInfo
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Abstract
A quinoa CqALS gene mutant and a molecular identification method and application thereof, wherein the quinoa CqALS2-1 gene mutant is quinoa CqALS-5, which is obtained by mutating G at 1609 th position of quinoa CqALS2-1 gene into T, so that the quinoa CqALS gene mutant generates resistance to chemical medicines (ASL) herbicides. The nucleotide sequence of CqALS2-1 is shown as SEQ ID No. 1. After the gene of the quinoa CqALS gene mutant CqALS-5 is mutated, the herbicide resistance of quinoa is obviously improved, a good model is provided for large-scale production of quinoa, the production cost can be saved by using the herbicide, the use of labor force is reduced, and a certain foundation is laid for large-scale production of quinoa.
Description
Technical Field
The invention relates to the technical field of molecular biology, in particular to a quinoa CqALS gene mutant, a molecular identification method and application.
Background
Quinoa (chenopodium quintoaawilld), belonging to the family chenopodiaceae, dicotyledonous, 4 ploidy, annual herbaceous, united nations grain and agriculture organization (FAO) denominated 2013 as international quinoa year, promoting quinoa worldwide. In 2020, more than 100 countries are used for planting experiments or commercial production of quinoa, but one of the biggest problems encountered in quinoa production is that various weeds such as brassica oleracea, rape and the like which are not registered yet can be removed in a suitable way, the problem of weeds is solved mainly by manual weeding in China, the labor cost per hectare of the production cost is increased by 4500 primordial and the production cost of 2021 is increased, besides the cost is increased, the risk of insufficient labor is often caused in weeding seasons in quinoa centralized production areas, the weed treatment in quinoa scale production becomes an important limiting factor, the production cost is increased, and the consumption end is influenced.
Acetolactate synthase (ALS), an ALS, is present in the plant growth process and catalyzes the synthesis of branched-chain amino acids by catalyzing the synthesis of acetolactate with high specificity and extremely high catalytic efficiency. Most of the existing herbicide components in the market are ALS inhibitors, and the synthesis of valine, leucine and isoleucine in plants is destroyed by inhibiting ALS, but the ALS inhibitors are effective for weeds and killing quinoa.
Development and cultivation of quinoa varieties capable of resisting herbicide become key to solving large-scale production of quinoa, and are the most effective and direct way for reducing production cost.
The invention realizes point mutation on quinoa seed genome by using an EMS mutagenesis mode, finds out a quinoa CqALS gene mutant which can have resistance to ALS inhibitor herbicides, and provides a quinoa CqALS gene mutant, a molecular identification method and application.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide the quinoa material with herbicide resistance.
In order to solve the technical problems, the invention adopts the following technical scheme:
a construction method of quinoa CqALS gene mutant comprises the following steps:
s1, preparing EMS with the concentration of 1.5 mg/ml;
s2, selecting quinoa seeds with the particle diameter of more than 2.5mm and the milky color, which are suitable for highland growth, and soaking the quinoa seeds in EMS solution with the concentration of 1.5mg/ml for 15min.
S3, sowing and raising the treated quinoa seeds in a greenhouse by adopting a seedling raising tray in time, transplanting the seedlings in a prepared field after the seedlings grow to 6 leaves, wherein the transplanting density is 40cm in row spacing and 20cm in plant spacing, managing the seedlings according to normal quinoa planting in the later period, and harvesting single plant seeds after the seedlings are ripe and recording the single plant seeds as second-generation seeds.
S4, sowing the second generation seeds in a plug tray with the length, width and height (30 cm multiplied by 60cm multiplied by 5 cm), cultivating to grow 4 leaves, and spraying 5 times of recommended use concentration of Imazamox (Imazamox) herbicide ALS herbicide with the concentration of (23 mg/m 2 ) Daily changes are recorded by observation, normal management is carried out every day, surviving individuals are marked after 15 days, the individuals which are not affected by herbicide are selected to be transplanted to a seedling raising basin (size) after 20 days, the seedlings are cultivated until the seeds are mature, and the seeds are harvested (third generation seeds).
S5, after the seeds of the second generation knot are mature, collecting single plant seeds and marking the single plant seeds as third generation seeds, and screening quinoa genotypes with target herbicide resistance by using the third generation seeds;
s6, identifying CqALS gene mutants.
A quinoa CqALS gene mutant, wherein the quinoa CqALS gene mutant CqALS-5 is a mutant with herbicide resistance formed after the quinoa CqALS2-1 gene is subjected to point mutation.
The seeds used in the construction of the quinoa variants were seeds of the tieba 505 material in the shanxi nong Gu Jiaqi industry limited seed bank (this material belongs to the plateau variety, and the seed particles are white and large).
The mutation position of the quinoa CqALS gene mutant is that the G at 1609 th position on the nucleotide sequence of the quinoa CqALS gene is mutated into T.
The gene mutated into the quinoa CqALS-5 is CqALS2-1, and because quinoa is tetraploid, the CqALS genes comprise CqALS1-1, cqALS1-2, cqALS2-1 and CqALS2-2 which are four different CqALS genes, and the mutated CqALS2-1 has a nucleotide sequence of SEQ ID NO:1.
The gene nucleotide sequence of the quinoa CqALS gene mutant quinoa CqALS-5 is SEQ ID NO. 2, and is a mutant sequence obtained by mutating the sequence of SEQ ID NO.1 into T through G at 1609 th position.
The amino acid sequence formed by quinoa CqALS-5 is SEQ ID NO. 3.
The quinoa CqALS-5 mutant is obtained by EMS mutagenesis.
The invention also provides a molecular identification method of the quinoa CqALS gene mutant, which comprises the step of identifying the quinoa CqALS-5 mutant.
The mode used for identifying the quinoa CqALS-5 mutant is as follows: after extracting quinoa single plant DNA, designing a primer for amplification, and judging the mutation condition of the quinoa single plant.
The primer sequence used for identifying the quinoa CqALS-5 mutant is SEQ ID NO. 4-5.
The invention also provides application of the quinoa CqALS gene mutant, which comprises application of the quinoa CqALS-5 mutant strain in CqALS gene mutation and application of the quinoa CqALS-5 mutant strain in herbicide resistance.
The application of the quinoa CqALS-5 mutant strain in CqALS gene mutation, which is the application in the use of the CqALS gene mutation in the seeds.
The application of the quinoa CqALS-5 mutant strain in herbicide resistance is the application in herbicide resistance in the seed.
The application of the quinoa CqALS-5 mutant strain in herbicide resistance is the application in the application of the herbicide resistance to the prasite in the seeds.
Compared with the prior art, the invention has the following beneficial effects:
the invention realizes point mutation on quinoa seed genome by using an EMS mutagenesis mode, finds out a quinoa CqALS gene mutant which can resist CqALS inhibitor herbicides, especially ALS herbicides, provides a quinoa material with the performance of resisting a prasite herbicide, solves the problem of large scale of quinoa by selecting an excellent strain to cultivate the herbicide-resistant variety, and provides a backbone selfing line for future quinoa hybridization herbicide-resistant variety. The herbicide can be used for solving the accompanying weeds in the production of quinoa, so that the production cost of quinoa can be greatly reduced.
Drawings
FIG. 1 is a diagram showing the identification of CqALS2-1 gene mutants in the examples of the present invention;
FIG. 2 is a diagram of the normal CqALS2-1 gene structure;
FIG. 3 is a diagram showing the structure of the gene after mutation of the CqALS2-1 gene;
FIG. 4 shows the survival of quinoa CqALS gene mutant strain and normal quinoa strain under the action of a Purster herbicide;
FIG. 5 shows the sequencing results of quinoa CqALS2-1 gene mutant strain;
FIG. 6 is a diagram showing the result of the mutant gene in NCBI in quinoa CqALS2-1 gene mutant strain;
FIG. 7 is a schematic diagram of the gene mutation sites in quinoa CqALS2-1 gene mutant.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and explanation only and is not intended to limit the present invention.
Examples
In a specific embodiment of the invention, a specific construction method of a quinoa CqALS gene mutant is provided, and the construction method comprises the following steps:
EMS with the concentration of 1.5mg/ml is prepared, quinoa seeds with the particle diameter of more than 2.5mm and the color and luster of milky white are selected, the quinoa seeds suitable for the plateau growth are soaked in EMS with the concentration of 1.5mg/ml for 15min, the treated quinoa seeds (the seeds are marked as M1 generation seeds at the moment) are sowed in prepared seedling trays (the matrix is a special seedling raising matrix), the sowing density is 1000 seeds per tray, field transplanting is carried out after seedlings grow to 6 true leaves, the transplanting density is 40cm in row spacing and 20cm in plant spacing, normal management such as field fertilization, weeding, watering and the like is carried out according to the growth requirements of quinoa until quinoa is mature, each quinoa is numbered, the harvested individual plants are filled into a gauze bag, the quinoa is manually threshed, the quinoa seeds are treated cleanly, a is aired, a seed pool is put into M2 generation seeds when the moisture is 12%, the individual plants of each harvested individual plants are mixed with 300 grains, a mixed pool of M2 generation seeds is established for herbicide resistance test, the mixed mutants are sowed in greenhouse tray, and the mixed well mixed seeds are sowed in the hole tray, and the amount of the mixed mutants is 200 thousands of grains. Watering after emergence of seedlings, normally managing pest control, spraying a weed killer prasite after 4 leaves, preparing a stock solution with the concentration of 5X, (uniformly spraying by using a knapsack sprayer), selecting strong seedling single plants on the 15 th day after spraying, transferring the strong seedling single plants into a 20cm caliber seedling tray, culturing in a greenhouse until the quinoa is mature, numbering each quinoa, harvesting the single plant seeds and marking the single plant seeds as M3 generation seeds. Performing genotype identification of quinoa with CqALS gene mutation on the harvested single plant seeds, extracting DNA and passing through a specific primer upstream primer and a specific primer downstream primer: and (3) carrying out PCR, carrying out homozygous heterozygous identification of CqALS gene mutation on seeds with positive results, extracting DNA and carrying out DNA extraction through specific primers: and (3) performing PCR, wherein the seeds with positive results are stable quinoa materials with herbicide resistance.
In yet another specific embodiment of the present invention, the screening for the CqALS gene mutation is specifically:
the M2 generation seeds are used for establishing a mutant mixed pool for herbicide resistance ALS herbicide test, mixed mutant seeds are continuously sown in greenhouse trays, each tray is 600-1000, and the sowing amount is 200 ten thousand. Watering after emergence of seedlings, and normally managing pest control, spraying a weed removing agent prasite after 4 leaves, wherein the specific medicine screen concentration of the prasite is 16 g/mu, and the application time is once a day for 15 days. In the normal quinoa production process, the specific medicine sieve concentration of the prasite is 3.2 g/mu.
In yet another specific embodiment of the present invention, the genotyping of quinoa with the CqALS gene mutation is specifically:
the strain treated by EMS and screened by herbicide ALS herbicide drug is subjected to DNA extraction and amplified by specific primers, and CqALS gene mutant is screened, wherein the primer sequence designed for CqALS2-1 gene mutant gene is as follows: f5 '-CATTACTCCTCAATATGACCCTA-3' R5 '-TCTTCTTGCTCACCCTTTC-3' (SEQ ID NO: 4-5). The quinoa CqALS-5 mutant is characterized in that the mutation position is that the G mutation at 1609 th position on the nucleotide sequence of quinoa CqALS2-1 gene is T, and the nucleotide sequence is SEQ ID NO. 2.
Finally, it should be noted that: the foregoing is merely a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is obvious to those skilled in the art that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made thereto. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Sequence listing
<110> Shanxinong Gu Jiaqi industries, inc
<120> quinoa CqALS gene mutant and molecular identification method and application
<141> 2022-02-12
<160> 5
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1860
<212> DNA
<213> Chen-Mai 505 (unknown)
<400> 1
atggcaattg ctgcttctaa tctcctcttc tcaaaaccac tcttaactac taattcctca 60
ttaaccctta ataactatct ttccaacaac aaaattatca catgttcttt acataaccaa 120
aaaccaaagc aatataaggc caatacaacc tctaattcct ccattaccat atcttcacaa 180
aaccccatta ctcctcaata tgaccctaat gagccccgta agacagccga cgtgctaatt 240
gaagccctcg agcgggaagg ggtgaccgat gtgtttgcct acccaggtgg ggcgtcaata 300
gaaatccatc aagctttgac taaatccaaa accatcaata caatcctccc tcgccacgag 360
cagggaggaa tcttagctag tgtaggctat gcacgcgcct cggatagacc aggcgtgtgc 420
ataaccacgt ctggccccgg agcaactaat cttgtcactg gactagctga tgcaatgacg 480
gacagtgtcc ccgttattgc tatcacgggt caagtgcccc gtaaattgat tggcactaat 540
gctttccaag aggttccaat aacagatatt gcaaaaagta ttactaagca tgtttacctt 600
gtacttgact cgaatgacat accttggatt gccaatgaag cctttattat tgcaaccacg 660
ggccgccctg gacccgtgtt gatcgacatt cctaaggatg ttcaacaaga aataatagtc 720
cctcgatggt tagaccattc tattccccaa gggtacattt ctcgattacc aaaaccgcct 780
aaaatcgagg cggtccataa aatcgtggaa atgatttttg aggctaagaa gccggtgttg 840
tacataggag ggggttgtgt aaatgcaagc gaggaactaa ggaggtttgt tgaattaacc 900
ggtatacccg ttgcgagtac attgatgggt ttaggagttt tctcgccgta tgataataat 960
gataataatt gtcatgaaat gtcactagga atgctaggga tgcatggtac aacatatgcc 1020
aattacgcaa ttgataaatc agatttgttg ttggcttttg gggtaaggtt tgatgaccga 1080
gtgacgggca aaatcgaggc gtttgcaagc cgagctaaga ttgtccacat caacatcgat 1140
gctcgtgagc taggaaagaa caagcagccc catgtctcga tgcacaatga cataaagata 1200
gcattacaag taatcaacta tatcttggca aaaagagggg aaacctcgaa aaaggactat 1260
ttcttagagt ggaggaatga gctacgaaaa ctaaaactaa atagccatcc aacaaacaat 1320
aatgttgtct acgatgacac aatacaatct cagtatgcca tcgaggtcct agacgaatta 1380
accaaaggaa gcgcgattat aaccacagga gtcggacaac accaaatatt tgtcgcgcat 1440
tattacaaat ttaagtcccc tcgccaatgg ataacctcag gagggatagg cacaatgggg 1500
tacggccttc cagcagcaat gggagtcgca gttgcaaaac cggggagcct agtcatagac 1560
gtcgatgggg atggaagtat ggtggttcaa gaggaagata tgttttacga agggaatcga 1620
gcacaatcat ttttagggaa cccgagaaaa gagggttact tgttccctga catggttaaa 1680
tttgctgagg cgtgtgacat tccgggtgaa agggtgagca agaagagcga gcttcgccaa 1740
gcaatcgaaa gaatgctgaa aactccagga ccatacttgc ttgatgttgg tgttgcacac 1800
caagaacatg tcttacctat gattcctagt ggtgcaacgt ttgctgatac cattaccgag 1860
<210> 2
<211> 1860
<212> DNA
<213> Chen's wheat 505 mutant (unknown)
<400> 2
atggcaattg ctgcttctaa tctcctcttc tcaaaaccac tcttaactac taattcctca 60
ttaaccctta ataactatct ttccaacaac aaaattatca catgttcttt acataaccaa 120
aaaccaaagc aatataaggc caatacaacc tctaattcct ccattaccat atcttcacaa 180
aaccccatta ctcctcaata tgaccctaat gagccccgta agacagccga cgtgctaatt 240
gaagccctcg agcgggaagg ggtgaccgat gtgtttgcct acccaggtgg ggcgtcaata 300
gaaatccatc aagctttgac taaatccaaa accatcaata caatcctccc tcgccacgag 360
cagggaggaa tcttagctag tgtaggctat gcacgcgcct cggatagacc aggcgtgtgc 420
ataaccacgt ctggccccgg agcaactaat cttgtcactg gactagctga tgcaatgacg 480
gacagtgtcc ccgttattgc tatcacgggt caagtgcccc gtaaattgat tggcactaat 540
gctttccaag aggttccaat aacagatatt gcaaaaagta ttactaagca tgtttacctt 600
gtacttgact cgaatgacat accttggatt gccaatgaag cctttattat tgcaaccacg 660
ggccgccctg gacccgtgtt gatcgacatt cctaaggatg ttcaacaaga aataatagtc 720
cctcgatggt tagaccattc tattccccaa gggtacattt ctcgattacc aaaaccgcct 780
aaaatcgagg cggtccataa aatcgtggaa atgatttttg aggctaagaa gccggtgttg 840
tacataggag ggggttgtgt aaatgcaagc gaggaactaa ggaggtttgt tgaattaacc 900
ggtatacccg ttgcgagtac attgatgggt ttaggagttt tctcgccgta tgataataat 960
gataataatt gtcatgaaat gtcactagga atgctaggga tgcatggtac aacatatgcc 1020
aattacgcaa ttgataaatc agatttgttg ttggcttttg gggtaaggtt tgatgaccga 1080
gtgacgggca aaatcgaggc gtttgcaagc cgagctaaga ttgtccacat caacatcgat 1140
gctcgtgagc taggaaagaa caagcagccc catgtctcga tgcacaatga cataaagata 1200
gcattacaag taatcaacta tatcttggca aaaagagggg aaacctcgaa aaaggactat 1260
ttcttagagt ggaggaatga gctacgaaaa ctaaaactaa atagccatcc aacaaacaat 1320
aatgttgtct acgatgacac aatacaatct cagtatgcca tcgaggtcct agacgaatta 1380
accaaaggaa gcgcgattat aaccacagga gtcggacaac accaaatatt tgtcgcgcat 1440
tattacaaat ttaagtcccc tcgccaatgg ataacctcag gagggatagg cacaatgggg 1500
tacggccttc cagcagcaat gggagtcgca gttgcaaaac cggggagcct agtcatagac 1560
gtcgatgggg atggaagtat ggtggttcaa gaggaagata tgttttacta agggaatcga 1620
gcacaatcat ttttagggaa cccgagaaaa gagggttact tgttccctga catggttaaa 1680
tttgctgagg cgtgtgacat tccgggtgaa agggtgagca agaagagcga gcttcgccaa 1740
gcaatcgaaa gaatgctgaa aactccagga ccatacttgc ttgatgttgg tgttgcacac 1800
caagaacatg tcttacctat gattcctagt ggtgcaacgt ttgctgatac cattaccgag 1860
<210> 3
<211> 536
<212> PRT
<213> Chen's wheat 505 mutant (unknown)
<400> 3
Met Ala Ile Ala Ala Ser Asn Leu Leu Phe Ser Lys Pro Leu Leu Thr
1 5 10 15
Thr Asn Ser Ser Leu Thr Leu Asn Asn Tyr Leu Ser Asn Asn Lys Ile
20 25 30
Ile Thr Cys Ser Leu His Asn Gln Lys Pro Lys Gln Tyr Lys Ala Asn
35 40 45
Thr Thr Ser Asn Ser Ser Ile Thr Ile Ser Ser Gln Asn Pro Ile Thr
50 55 60
Pro Gln Tyr Asp Pro Asn Glu Pro Arg Lys Thr Ala Asp Val Leu Ile
65 70 75 80
Glu Ala Leu Glu Arg Glu Gly Val Thr Asp Val Phe Ala Tyr Pro Gly
85 90 95
Gly Ala Ser Ile Glu Ile His Gln Ala Leu Thr Lys Ser Lys Thr Ile
100 105 110
Asn Thr Ile Leu Pro Arg His Glu Gln Gly Gly Ile Leu Ala Ser Val
115 120 125
Gly Tyr Ala Arg Ala Ser Asp Arg Pro Gly Val Cys Ile Thr Thr Ser
130 135 140
Gly Pro Gly Ala Thr Asn Leu Val Thr Gly Leu Ala Asp Ala Met Thr
145 150 155 160
Asp Ser Val Pro Val Ile Ala Ile Thr Gly Gln Val Pro Arg Lys Leu
165 170 175
Ile Gly Thr Asn Ala Phe Gln Glu Val Pro Ile Thr Asp Ile Ala Lys
180 185 190
Ser Ile Thr Lys His Val Tyr Leu Val Leu Asp Ser Asn Asp Ile Pro
195 200 205
Trp Ile Ala Asn Glu Ala Phe Ile Ile Ala Thr Thr Gly Arg Pro Gly
210 215 220
Pro Val Leu Ile Asp Ile Pro Lys Asp Val Gln Gln Glu Ile Ile Val
225 230 235 240
Pro Arg Trp Leu Asp His Ser Ile Pro Gln Gly Tyr Ile Ser Arg Leu
245 250 255
Pro Lys Pro Pro Lys Ile Glu Ala Val His Lys Ile Val Glu Met Ile
260 265 270
Phe Glu Ala Lys Lys Pro Val Leu Tyr Ile Gly Gly Gly Cys Val Asn
275 280 285
Ala Ser Glu Glu Leu Arg Arg Phe Val Glu Leu Thr Gly Ile Pro Val
290 295 300
Ala Ser Thr Leu Met Gly Leu Gly Val Phe Ser Pro Tyr Asp Asn Asn
305 310 315 320
Asp Asn Asn Cys His Glu Met Ser Leu Gly Met Leu Gly Met His Gly
325 330 335
Thr Thr Tyr Ala Asn Tyr Ala Ile Asp Lys Ser Asp Leu Leu Leu Ala
340 345 350
Phe Gly Val Arg Phe Asp Asp Arg Val Thr Gly Lys Ile Glu Ala Phe
355 360 365
Ala Ser Arg Ala Lys Ile Val His Ile Asn Ile Asp Ala Arg Glu Leu
370 375 380
Gly Lys Asn Lys Gln Pro His Val Ser Met His Asn Asp Ile Lys Ile
385 390 395 400
Ala Leu Gln Val Ile Asn Tyr Ile Leu Ala Lys Arg Gly Glu Thr Ser
405 410 415
Lys Lys Asp Tyr Phe Leu Glu Trp Arg Asn Glu Leu Arg Lys Leu Lys
420 425 430
Leu Asn Ser His Pro Thr Asn Asn Asn Val Val Tyr Asp Asp Thr Ile
435 440 445
Gln Ser Gln Tyr Ala Ile Glu Val Leu Asp Glu Leu Thr Lys Gly Ser
450 455 460
Ala Ile Ile Thr Thr Gly Val Gly Gln His Gln Ile Phe Val Ala His
465 470 475 480
Tyr Tyr Lys Phe Lys Ser Pro Arg Gln Trp Ile Thr Ser Gly Gly Ile
485 490 495
Gly Thr Met Gly Tyr Gly Leu Pro Ala Ala Met Gly Val Ala Val Ala
500 505 510
Lys Pro Gly Ser Leu Val Ile Asp Val Asp Gly Asp Gly Ser Met Val
515 520 525
Val Gln Glu Glu Asp Met Phe Tyr
530 535
<210> 4
<211> 23
<212> DNA
<213> unknown
<400> 4
cattactcct caatatgacc cta 23
<210> 5
<211> 19
<212> DNA
<213> unknown
<400> 5
tcttcttgct caccctttc 19
Claims (6)
1. A quinoa CqALS-5 mutant of quinoa CqALS gene is characterized in that the mutation position is that the G mutation at 1609 th position on the nucleotide sequence of quinoa CqALS2-1 gene is T, and the nucleotide sequence is SEQ ID NO. 2.
2. A molecular identification method of quinoa CqALS gene mutant is characterized in that the method can rapidly identify the sequence of SEQ ID NO. 2 so as to determine the quinoa CqALS gene mutant.
3. The method for molecular identification of quinoa CqALS gene mutant according to claim 2, characterized in that the method for identifying quinoa CqALS-5 mutant is as follows: after extracting quinoa single plant DNA, designing a specific primer for amplification, and judging the mutation condition of the quinoa single plant, wherein the mutation position of the quinoa CqALS-5 mutant is that the G mutation at 1609 th position on the nucleotide sequence of quinoa CqALS2-1 gene is T, and the nucleotide sequence is SEQ ID NO. 2.
4. A molecular identification method of quinoa CqALS gene mutant according to claim 3, wherein the primer sequence used for identifying quinoa CqALS-5 mutant is SEQ ID NO. 4-5.
5. The application of a quinoa CqALS gene mutant is characterized in that the application of a quinoa CqALS-5 mutant strain in CqALS gene mutation is characterized in that the mutation position of the quinoa CqALS-5 mutant strain is that the G at 1609 th position on the nucleotide sequence of the quinoa CqALS2-1 gene is T, and the nucleotide sequence is SEQ ID NO. 2.
6. The use of a quinoa CqALS gene mutant according to claim 5, characterized in that the quinoa CqALS-5 mutant is used against a prasite herbicide.
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| CN106591334A (en) * | 2016-12-19 | 2017-04-26 | 江苏省农业科学院 | Wheat ALS mutant gene and application thereof in resisting herbicide |
| CN106755019A (en) * | 2016-12-19 | 2017-05-31 | 江苏省农业科学院 | A kind of application of wheat ALS mutated genes and its albumen in terms of antiweed |
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| CN107354139A (en) * | 2017-09-18 | 2017-11-17 | 江苏省农业科学院 | Wheat ALS muteins, nucleic acid and its application |
| CN113249351A (en) * | 2020-02-07 | 2021-08-13 | 山东舜丰生物科技有限公司 | Herbicide-resistant gene, polypeptide and application thereof in plant breeding |
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| CN106591334A (en) * | 2016-12-19 | 2017-04-26 | 江苏省农业科学院 | Wheat ALS mutant gene and application thereof in resisting herbicide |
| CN106755019A (en) * | 2016-12-19 | 2017-05-31 | 江苏省农业科学院 | A kind of application of wheat ALS mutated genes and its albumen in terms of antiweed |
| CN107022540A (en) * | 2017-06-14 | 2017-08-08 | 江苏省农业科学院 | Make plant that there is wheat ALS muteins, gene and its application of Herbicid resistant |
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