CN114540295A - Culture medium for culturing mesenchymal stem cells - Google Patents
Culture medium for culturing mesenchymal stem cells Download PDFInfo
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- CN114540295A CN114540295A CN202210251750.0A CN202210251750A CN114540295A CN 114540295 A CN114540295 A CN 114540295A CN 202210251750 A CN202210251750 A CN 202210251750A CN 114540295 A CN114540295 A CN 114540295A
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- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 36
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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Abstract
The invention belongs to the technical field of culture media, and particularly relates to a culture medium for culturing mesenchymal stem cells, which comprises a basic culture medium, insulin analogues, cholesterol, transdermal polypeptide, vitronectin, recombinant human transferrin, defatted perilla seed protein, stilbene glucoside, resveratrol derivatives and ginkgo leaf extract; through setting up the subassembly of stirring between regulation dish and activity dish, when the activity dish rotated, fixed support the piece can with the elastic plate contact, and then make the elastic plate inwards shrink, the spheroid at the inboard middle part of elastic plate can block in the recess in the perforated plate simultaneously, in the in-process of perforated plate and separation perforated plate is gone into to the spheroid card, the culture medium on the activity dish produces the fine motion in addition, and then makes the nutrient composition in the culture medium mix more evenly.
Description
Technical Field
The invention belongs to the technical field of culture media, and particularly relates to a culture medium for culturing mesenchymal stem cells.
Background
Mesenchymal stem cells are adult stem cells with plasticity, which are widely found in bone marrow, placenta, umbilical cord and amniotic fluid.
In the prior art, during the culture of the mesenchymal stem cells, various nutrient substances are required to be added into a culture medium to simulate the growth environment of the mesenchymal stem cells in a living body.
When the existing culture medium for culturing the mesenchymal stem cells is prepared, the culture medium added with various nutrients needs to be uniformly mixed, the traditional mixing mode can only be generally carried out by rotation, the rotation of the traditional mode rotates at a certain speed and direction, and the components in the culture medium cannot be uniformly mixed, so that the culture effect of the culture medium is poor; to this end, the invention provides a culture medium for culturing mesenchymal stem cells.
Disclosure of Invention
In order to make up for the defects of the prior art and solve the problem that the traditional mixing mode can only be carried out through rotation generally, the rotation of the traditional mode rotates at a certain speed and direction, and the components in the culture medium cannot be mixed uniformly, so that the culture effect of the culture medium is poor, the invention provides the culture medium for culturing the mesenchymal stem cells.
The technical scheme adopted by the invention for solving the technical problems is as follows: a culture medium for culturing mesenchymal stem cells comprises basal medium, insulin analogue, cholesterol, transdermal polypeptide, vitronectin, recombinant human transferrin, defatted perillaseed protein, stilbene glucoside, resveratrol derivative and folium Ginkgo extract;
the dosage of each component is respectively as follows according to the final concentration of the culture medium: insulin analog 6.7-8.9 mg/L; 1.7-3.6mg/L of cholesterol, 22.5-24.6 μ g/L of transdermal polypeptide, 15.7-17.1 μ g/L of vitronectin, 8.1-12.1mg/L of recombinant human transferrin, 5.2-7.8mg/L of defatted perilla seed protein, 8.7-9.3 μ g/L of stilbene glucoside, 6.0-8.3 μ g/L of resveratrol derivative and 7.6-8.9 μ g/L of ginkgo biloba extract.
Preferably, the basic culture medium is DMEM/F12 culture medium.
Preferably, the mesenchymal stem cells are placental mesenchymal stem cells obtained from a placenta.
Preferably, the preparation method of the medium for culturing mesenchymal stem cells comprises the following steps:
s1: adding insulin analogue, cholesterol transdermal polypeptide, vitronectin, recombinant human transferrin, defatted perilla seed protein, stilbene glucoside, resveratrol derivative and ginkgo biloba extract into a basic culture medium;
s2: placing the basic culture medium added with various raw materials on a shaking mechanism, and starting the shaking mechanism to uniformly mix the liquid in the basic culture medium;
s3: and S2, uniformly mixing the basic culture medium, and filtering and sterilizing to obtain the serum-free culture medium for culturing the mesenchymal stem cells.
Preferably, in S3, the filter sterilization is performed by filtration through a filter membrane, and the pore size of the filter membrane is 0.20-0.25 μm.
Preferably, the shaking mechanism consists of a base, a fixed disc, a driving assembly, a movable disc and a shifting assembly; the fixed disc is fixedly connected to the base; the movable disc is arranged on the fixed disc and rotates on the fixed disc through the driving assembly; the driving assembly is arranged in the base, and the shifting assembly is arranged between the fixed disc and the movable disc; the door groove has been seted up on the base, just the rigid coupling has the stopper in the door groove, simultaneously, the subassembly of stirring between fixed disk and activity dish can indirectly act on the activity dish, and then makes the change that the activity dish produced speed, is favorable to changing the removal orbit of the inside nutrient composition of culture medium, and then is favorable to the mixing, and the door groove of arranging on the base is used for placing the door plant, and the stopper of door inslot is used for spacing the door plant.
Preferably, the driving assembly consists of a motor, a duplicate gear, a rotating shaft and a duplicate gear; the motor is arranged in the base, and the linkage gear is fixedly connected to an output shaft of the motor; one end of the rotating shaft penetrates through the base and is fixedly connected to the middle part of the movable disc; the other end rigid coupling duplicate gear of pivot, just duplicate gear with the linkage gear meshing, the pivot rotates then can indirectly drive the movable plate and rotate on the fixed disk, consequently realizes driving the culture medium rotation on the movable plate, is favorable to the nutrient composition mixing in the culture medium.
Preferably, the rigid coupling has a set casing on the surface of activity dish, just can dismantle on the extension line of set casing and be connected with the clamp, the set casing that sets up on the activity dish is used for fixed culture medium, and the clamp of arranging on the set casing extension line is used for carrying out further spacing to the culture medium.
Preferably, the poking assembly consists of an elastic plate, an elastic rod, a connecting shaft, a sphere and a fixed abutting block; the elastic rod is fixedly connected to the side wall of the movable plate, and the other end of the elastic rod is fixedly connected with the elastic plate; the elastic plates are arranged in six groups, and the six groups of elastic plates are circumferentially and symmetrically surrounded on the side wall of the movable disc; the connecting shaft is fixedly connected to the middle of the inner side of the elastic plate, and the ball body is fixedly connected to the bottom end of the connecting shaft; the fixed abutting blocks are correspondingly provided with six groups, the six groups of fixed abutting blocks are symmetrically and fixedly connected to the fixed disc in a circumferential symmetrical mode, when the ball body enters the groove and is separated from the groove, the ball body vibrates the movable disc to a certain degree, so that the moving track instability of nutrient components in the culture medium can be caused, and the nutrient components in the culture medium can be effectively and uniformly mixed at the moment.
Preferably, the inside of activity dish is equipped with the perforated plate, just corresponding to spheroidal recess has been seted up to the inside of perforated plate, and the perforated plate mainly is the porous resin structure, and it can produce elastic deformation under the external force extrusion, and sets up the recess in the perforated plate, then is used for spacing to the spheroid.
The invention has the technical effects and advantages that:
1. according to the culture medium for culturing the mesenchymal stem cells, the movable disc is driven to move on the fixed disc through the driving assembly, so that when the motor drives the linkage gear to rotate, the movable disc is driven to rotate through the duplicate gear, the culture medium in the fixed shell can rotate, nutrient components in the culture medium can be mixed, the movable disc and the fixed disc are matched with the shifting assembly arranged between the movable disc and the fixed disc, the movable disc is easily influenced by the fixed abutting block to decelerate when rotating, and therefore the nutrient components in the culture medium on the movable disc can be mixed more uniformly.
2. According to the culture medium for culturing the mesenchymal stem cells, the toggle assembly arranged between the specified plate and the movable plate is adopted, when the movable plate rotates, the fixed abutting block can be in contact with the elastic plate, so that the elastic plate contracts inwards, meanwhile, the ball body in the middle of the inner side of the elastic plate can be clamped into the groove in the porous plate, and in the process that the ball body is clamped into the porous plate and separated from the porous plate, the culture medium on the movable plate generates micro-motion, so that the nutrient components in the culture medium are mixed more uniformly.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a flow chart of a production process in the present invention;
FIG. 2 is a perspective view of the shake-up mechanism of the present invention;
FIG. 3 is a top view of the shake-up mechanism of the present invention;
FIG. 4 is a side view of the rock-up mechanism of the present invention;
FIG. 5 is a cross-sectional view of the shake-up mechanism of the present invention;
FIG. 6 is a front view of the movable plate of the present invention;
fig. 7 is an enlarged schematic view of a portion a of fig. 6.
In the figure: 1. a base; 11. fixing the disc; 111. fixing the resisting block; 12. a door slot; 13. a limiting block; 21. a movable tray; 211. an elastic plate; 212. an elastic rod; 213. a perforated plate; 214. a groove; 215. a connecting shaft; 216. a sphere; 22. a stationary case; 23. clamping a hoop; 24. a rotating shaft; 25. a duplicate gear; 3. a motor; 31. a linkage gear.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 7, a culture medium for culturing mesenchymal stem cells according to the present invention comprises a basal medium, insulin analogues, cholesterol, transdermal polypeptide, vitronectin, recombinant human transferrin, defatted perilla seed protein, stilbene glycoside, resveratrol derivatives and ginkgo biloba extract;
the dosage of each component is respectively as follows according to the final concentration of the culture medium: insulin analog 7.6 mg/L; 2.8mg/L of cholesterol, 23.5 mu g/L of transdermal polypeptide, 16.5 mu g/L of vitronectin, 10.5mg/L of recombinant human transferrin, 6.6mg/L of defatted perilla seed protein, 8.9 mu g/L of stilbene glucoside, 7.3 mu g/L of resveratrol derivative and 8.3 mu g/L of ginkgo biloba extract.
As an embodiment of the invention, the basic medium is DMEM/F12 medium.
As an embodiment of the present invention, the mesenchymal stem cells are placental mesenchymal stem cells obtained from a placenta.
As one embodiment of the present invention, the method for preparing a medium for culturing mesenchymal stem cells comprises the steps of:
s1: adding insulin analogue, cholesterol transdermal polypeptide, vitronectin, recombinant human transferrin, defatted perilla seed protein, stilbene glucoside, resveratrol derivative and ginkgo biloba extract into a basic culture medium;
s2: placing the basic culture medium added with various raw materials on a shaking mechanism, and starting the shaking mechanism to uniformly mix the liquid in the basic culture medium;
s3: and S2, uniformly mixing the basic culture medium, and filtering and sterilizing to obtain the serum-free culture medium for culturing the mesenchymal stem cells.
In one embodiment of the present invention, in S3, the filtration sterilization is performed using a filter membrane, and the pore size of the filter membrane is 0.22 μm.
As an embodiment of the invention, the shaking mechanism consists of a base 1, a fixed disc 11, a driving component, a movable disc 21 and a toggle component; the fixed disc 11 is fixedly connected to the base 1; the movable disc 21 is arranged on the fixed disc 11, and the movable disc 21 rotates on the fixed disc 11 through the driving assembly; the driving assembly is arranged in the base 1, and the shifting assembly is arranged between the fixed disc 11 and the movable disc 21; a door slot 12 is formed in the base 1, and a limiting block 13 is fixedly connected in the door slot 12.
The during operation, when mixing the nutrient composition in the culture medium, artificial fixes the culture medium on activity dish 21, start drive assembly afterwards, utilize drive assembly to drive and produce the relative activity relation between activity dish 21 and the fixed disk 11, when activity dish 21 rotates, the nutrient composition of the culture medium inside that arranges on it can be along with activity dish 21 pivoted speed and direction production centrifugal effect, and then can realize the effect of mixing, and simultaneously, stir the subassembly between fixed disk 11 and activity dish 21, can indirectly act on activity dish 21, and then make activity dish 21 produce the change of speed, be favorable to changing the removal orbit of the nutrient composition of the culture medium inside, and then be favorable to the mixing, arrange door groove 12 on base 1 and be used for placing the door plant, and stopper 13 in the door groove 12 is used for spacing the door plant.
As an embodiment of the present invention, the driving assembly is composed of a motor 3, a duplicate gear 25, a rotating shaft 24 and a linkage gear 31; the motor 3 is arranged in the base 1, and the linkage gear 31 is fixedly connected to an output shaft of the motor 3; one end of the rotating shaft 24 penetrates through the base 1 and is fixedly connected to the middle part of the movable disc 21; the other end of the rotating shaft 24 is fixedly connected with a duplicate gear 25, and the duplicate gear 25 is meshed with the linkage gear 31.
When the culture medium stirring device works, the driving assembly drives the linkage gear 31 to rotate by the motor 3, when the linkage gear 31 rotates, the linkage gear can drive the rotating shaft 24 to rotate by being meshed with the duplicate gear 25, and the rotating shaft 24 rotates to indirectly drive the movable disc 21 to rotate on the fixed disc 11, so that the culture medium on the movable disc 21 is driven to rotate, and the culture medium stirring device is favorable for uniformly mixing nutrient components in the culture medium.
In one embodiment of the present invention, a fixed case 22 is fixed to a surface of the movable plate 21, and a clip 23 is detachably connected to an extension line of the fixed case 22.
In operation, the fixed shell 22 that sets up on the activity dish 21 is used for fixed culture medium, and the clamp 23 of arranging on the extension line of fixed shell 22 is used for carrying out further spacing to the culture medium.
As an embodiment of the present invention, the toggle assembly is composed of an elastic plate 211, an elastic rod 212, a connecting shaft 215, a sphere 216 and a fixed resisting block 111; the elastic rod 212 is fixedly connected to the side wall of the movable plate, and the other end of the elastic rod 212 is fixedly connected to the elastic plate 211; six groups of elastic plates 211 are arranged, and the six groups of elastic plates 211 are circumferentially and symmetrically surrounded on the side wall of the movable disc 21; the connecting shaft 215 is fixedly connected to the middle of the inner side of the elastic plate 211, and the sphere 216 is fixedly connected to the bottom end of the connecting shaft 215; six groups of the fixed abutting blocks 111 are correspondingly arranged, and the six groups of the fixed abutting blocks 111 are circumferentially and symmetrically fixedly connected on the fixed disc 11.
In operation, when the movable plate 21 is rotated by the rotation shaft 24, it is rotated in the fixed plate 11, and when the movable plate 21 is rotated, the elastic plate 211 arranged on the side wall thereof will contact with the fixing resisting block 111 on the fixing disc 11, when the fixed block 111 contacts the elastic plate 211, the elastic plate 211 will generate a contraction effect, while the elastic rod 212 between the elastic plate 211 and the movable plate 21 is elastically deformed, for providing a supporting effect to the elastic plate 211, and during the inward contraction of the elastic plate 211, the connecting shaft 215 at the middle of the inner side of the movable plate drives the ball 216 to move inwards inside the movable plate 21, and the ball 216 is clamped in the groove 214 inside the porous plate 213, when the fixed resisting block 111 is separated from the elastic plate 211, under the driving of the elastic rod 212, the elastic plate 211 is reset, and at this time, the elastic plate 211 drives the ball 216 at the middle part thereof to separate from the groove 214; when fixed block 111 and the elastic plate 211 contact of propping, the speed of activity dish 21 can reduce, and fixed block 111 and the elastic plate 211 of propping of circumference symmetrical arrangement, can make the even reduction of speed of activity dish 21 reaccelerate, consequently, make the culture medium of activity dish 21 internal fixation, its inside nutrient composition misce bene, and when spheroid 216 gets into recess 214 and separates with recess 214, can produce the shock of certain degree to activity dish 21, consequently, can lead to the nutrient composition in the culture medium to remove the orbit unstability, can effectually at this moment be with the nutrient composition misce bene in the culture medium.
In one embodiment of the present invention, a porous plate 213 is disposed inside the movable plate 21, and a groove 214 corresponding to the ball 216 is disposed inside the porous plate 213.
In operation, the porous plate 213 is mainly made of porous resin and can be elastically deformed under the external force, and the groove 214 formed in the porous plate 213 is used for limiting the ball 216.
The working principle is as follows: the traditional mixing mode can only be performed by rotation generally, and the rotation of the traditional mode rotates at a certain speed and direction, so that the components in the culture medium can not be mixed uniformly, and the culture effect of the culture medium is poor;
when the device is used, firstly, nutrient components in a culture medium are mixed, the culture medium is artificially fixed on the movable disc 21, then the driving assembly is started, the driving assembly is utilized to drive the movable disc 21 and the fixed disc 11 to generate a relative activity relationship, when the movable disc 21 rotates, the nutrient components in the culture medium arranged on the movable disc 21 can generate a centrifugal effect along with the rotating speed and the rotating direction of the movable disc 21, and further the uniform mixing effect can be realized, meanwhile, the stirring assembly between the fixed disc 11 and the movable disc 21 can indirectly act on the movable disc 21, so that the movable disc 21 generates speed change, the moving track of the nutrient components in the culture medium can be favorably changed, and further the uniform mixing is favorably realized, wherein when the driving assembly works, the linkage gear 31 is driven by the motor 3 to rotate, and when the linkage gear 31 rotates, the rotation shaft 24 can be driven to rotate through the meshing with the duplicate gear 25, the rotation of the rotating shaft 24 indirectly drives the movable disc 21 to rotate on the fixed disc 11, so that the culture medium on the movable disc 21 is driven to rotate, and the nutrient components in the culture medium are uniformly mixed;
when the movable disk 21 is driven by the rotating shaft 24 to rotate, the movable disk 21 rotates in the fixed disk 11, when the movable disk 21 rotates, the elastic plate 211 arranged on the side wall of the movable disk is in contact with the fixed abutting block 111 on the fixed disk 11, when the fixed abutting block 111 is in contact with the elastic plate 211, the elastic plate 211 generates a contraction effect, meanwhile, the elastic rod 212 between the elastic plate 211 and the movable disk 21 generates elastic deformation for providing a supporting effect for the elastic plate 211, and in the inward contraction process of the elastic plate 211, the connecting shaft 215 in the middle of the inner side of the elastic plate can drive the ball 216 to move inwards in the movable disk 21, and the ball 216 is clamped in the groove 214 in the porous plate 213, when the fixed abutting block 111 is separated from the elastic plate 211, the elastic plate 211 is reset under the drive of the elastic rod 212, and at this time, the elastic plate 211 drives the ball 216 in the middle of the elastic plate to be separated from the groove 214; when the fixed resisting block 111 is contacted with the elastic plate 211, the speed of the movable disc 21 is reduced, and the fixed resisting block 111 and the elastic plate 211 which are symmetrically arranged on the circumference can uniformly reduce and accelerate the speed of the movable disc 21, so that the nutrient contents in the culture medium fixed in the movable disc 21 are uniformly mixed, and when the sphere 216 enters the groove 214 and is separated from the groove 214, the sphere can vibrate the movable disc 21 to a certain degree, so that the moving track of the nutrient contents in the culture medium is unstable, and the nutrient contents in the culture medium can be effectively and uniformly mixed;
the porous plate 213 is mainly made of porous resin and can elastically deform under external pressure, and the groove 214 formed in the porous plate 213 is used for limiting the ball 216.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A medium for culturing mesenchymal stem cells, characterized in that: comprises basal medium, insulin analog, cholesterol, transdermal polypeptide, vitronectin, recombinant human transferrin, defatted perilla seed protein, stilbene glucoside, resveratrol derivative and ginkgo leaf extract;
the dosage of each component is respectively as follows according to the final concentration of the culture medium: insulin analog 6.7-8.9 mg/L; 1.7-3.6mg/L of cholesterol, 22.5-24.6 μ g/L of transdermal polypeptide, 15.7-17.1 μ g/L of vitronectin, 8.1-12.1mg/L of recombinant human transferrin, 5.2-7.8mg/L of defatted perilla seed protein, 8.7-9.3 μ g/L of stilbene glucoside, 6.0-8.3 μ g/L of resveratrol derivative and 7.6-8.9 μ g/L of ginkgo biloba extract.
2. A culture medium for culturing mesenchymal stem cells according to claim 1, characterized in that: the basic culture medium is DMEM/F12 culture medium.
3. A culture medium for culturing mesenchymal stem cells according to claim 2, characterized in that: the mesenchymal stem cells are placenta mesenchymal stem cells obtained from placenta.
4. A culture medium for culturing mesenchymal stem cells according to claim 3, wherein: the preparation method of the culture medium for culturing the mesenchymal stem cells comprises the following steps:
s1: adding insulin analogue, cholesterol transdermal polypeptide, vitronectin, recombinant human transferrin, defatted perilla seed protein, stilbene glucoside, resveratrol derivative and ginkgo biloba extract into a basic culture medium;
s2: putting the basic culture medium added with various raw materials on a shaking mechanism, and starting the shaking mechanism to uniformly mix the liquid in the basic culture medium;
s3: and S2, uniformly mixing the basic culture medium, and filtering and sterilizing to obtain the serum-free culture medium for culturing the mesenchymal stem cells.
5. A culture medium for culturing mesenchymal stem cells according to claim 4, wherein: in the step S3, filtering and sterilizing by using a filter membrane, wherein the pore diameter of the filter membrane is 0.20-0.25 μm.
6. A culture medium for culturing mesenchymal stem cells according to claim 5, wherein: the shaking mechanism consists of a base (1), a fixed disc (11), a driving component, a movable disc (21) and a shifting component; the fixed disc (11) is fixedly connected to the base (1); the movable disc (21) is arranged on the fixed disc (11), and the movable disc (21) rotates on the fixed disc (11) through the driving assembly; the driving assembly is arranged in the base (1), and the shifting assembly is arranged between the fixed disc (11) and the movable disc (21); a door slot (12) is formed in the base (1), and a limiting block (13) is fixedly connected in the door slot (12).
7. A culture medium for culturing mesenchymal stem cells according to claim 6, wherein: the driving assembly consists of a motor (3), a duplicate gear (25), a rotating shaft (24) and a linkage gear (31); the motor (3) is arranged in the base (1), and the linkage gear (31) is fixedly connected to an output shaft of the motor (3); one end of the rotating shaft (24) penetrates through the base (1) and is fixedly connected to the middle part of the movable disc (21); the other end of the rotating shaft (24) is fixedly connected with a duplicate gear (25), and the duplicate gear (25) is meshed with the linkage gear (31).
8. A culture medium for culturing mesenchymal stem cells according to claim 7, wherein: the fixed shell (22) is fixedly connected to the surface of the movable disc (21), and a clamp (23) is detachably connected to an extension line of the fixed shell (22).
9. A culture medium for culturing mesenchymal stem cells according to claim 8, wherein: the toggle assembly consists of an elastic plate (211), an elastic rod (212), a connecting shaft (215), a sphere (216) and a fixed abutting block (111); the elastic rod (212) is fixedly connected to the side wall of the movable plate, and the other end of the elastic rod (212) is fixedly connected with the elastic plate (211); six groups of elastic plates (211) are arranged, and the six groups of elastic plates (211) are circumferentially and symmetrically arranged on the side wall of the movable disc (21); the connecting shaft (215) is fixedly connected to the middle of the inner side of the elastic plate (211), and the sphere (216) is fixedly connected to the bottom end of the connecting shaft (215); six groups of fixed abutting blocks (111) are correspondingly arranged, and the six groups of fixed abutting blocks (111) are circumferentially and symmetrically fixedly connected on the fixed disc (11).
10. A culture medium for culturing mesenchymal stem cells according to claim 9, wherein: the inside of activity dish (21) is equipped with perforated plate (213), just recess (214) corresponding to spheroid (216) are seted up to the inside of perforated plate (213).
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| CN116515747A (en) * | 2022-08-23 | 2023-08-01 | 深圳市茵冠生物科技有限公司 | Dental pulp mesenchymal stem cell culture method |
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