CN114539416A - Chromatographic purification process of bispecific antibody - Google Patents
Chromatographic purification process of bispecific antibody Download PDFInfo
- Publication number
- CN114539416A CN114539416A CN202011342663.3A CN202011342663A CN114539416A CN 114539416 A CN114539416 A CN 114539416A CN 202011342663 A CN202011342663 A CN 202011342663A CN 114539416 A CN114539416 A CN 114539416A
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- Prior art keywords
- elution buffer
- purification process
- chromatographic
- chromatographic purification
- bispecific antibody
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
- C07K16/303—Liver or Pancreas
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
- C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
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CN202011342663.3A CN114539416A (en) | 2020-11-26 | 2020-11-26 | Chromatographic purification process of bispecific antibody |
Applications Claiming Priority (1)
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CN202011342663.3A CN114539416A (en) | 2020-11-26 | 2020-11-26 | Chromatographic purification process of bispecific antibody |
Publications (1)
Publication Number | Publication Date |
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CN114539416A true CN114539416A (en) | 2022-05-27 |
Family
ID=81660722
Family Applications (1)
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CN202011342663.3A Pending CN114539416A (en) | 2020-11-26 | 2020-11-26 | Chromatographic purification process of bispecific antibody |
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CN (1) | CN114539416A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116655726A (en) * | 2023-07-31 | 2023-08-29 | 上海澳斯康生物制药有限公司 | Antibody purification method based on ion exchange chromatography |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103833852A (en) * | 2012-11-23 | 2014-06-04 | 上海市肿瘤研究所 | Bispecific antibody aiming at phosphatidylinositols protein polysaccharide-3 and T cell antigen |
CN103833855A (en) * | 2009-06-26 | 2014-06-04 | 瑞泽恩制药公司 | Readily isolated bispecific antibodies with native immunoglobulin format |
CN107556387A (en) * | 2016-06-30 | 2018-01-09 | 中国科学院深圳先进技术研究院 | Resisting GPC 3 and the double targeting antibodies of CD3 specificity, the minicircle dna containing this pair of targeting antibodies expression cassette and application |
US20180079797A1 (en) * | 2015-04-10 | 2018-03-22 | Adimab, Llc | Methods for purifying heterodimeric multispecific antibodies from parental homodimeric antibody species |
CN110066314A (en) * | 2019-04-01 | 2019-07-30 | 上海药明生物技术有限公司 | A kind of efficient affinity purification technique for improving polymer separation resolution ratio |
US20190256556A1 (en) * | 2016-06-17 | 2019-08-22 | Genentech, Inc. | Purification of multispecific antibodies |
CN111018969A (en) * | 2019-12-27 | 2020-04-17 | 上海药明生物技术有限公司 | Method for purifying bispecific antibody by light chain select combined chromatography |
CN111465619A (en) * | 2017-12-21 | 2020-07-28 | 豪夫迈·罗氏有限公司 | Combination therapy of tumor-targeted ICOS agonists with T cell bispecific molecules |
WO2020177627A1 (en) * | 2019-03-02 | 2020-09-10 | 上海一宸医药科技有限公司 | Bispecific antibody |
-
2020
- 2020-11-26 CN CN202011342663.3A patent/CN114539416A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103833855A (en) * | 2009-06-26 | 2014-06-04 | 瑞泽恩制药公司 | Readily isolated bispecific antibodies with native immunoglobulin format |
CN103833852A (en) * | 2012-11-23 | 2014-06-04 | 上海市肿瘤研究所 | Bispecific antibody aiming at phosphatidylinositols protein polysaccharide-3 and T cell antigen |
US20180079797A1 (en) * | 2015-04-10 | 2018-03-22 | Adimab, Llc | Methods for purifying heterodimeric multispecific antibodies from parental homodimeric antibody species |
US20190256556A1 (en) * | 2016-06-17 | 2019-08-22 | Genentech, Inc. | Purification of multispecific antibodies |
CN107556387A (en) * | 2016-06-30 | 2018-01-09 | 中国科学院深圳先进技术研究院 | Resisting GPC 3 and the double targeting antibodies of CD3 specificity, the minicircle dna containing this pair of targeting antibodies expression cassette and application |
CN111465619A (en) * | 2017-12-21 | 2020-07-28 | 豪夫迈·罗氏有限公司 | Combination therapy of tumor-targeted ICOS agonists with T cell bispecific molecules |
WO2020177627A1 (en) * | 2019-03-02 | 2020-09-10 | 上海一宸医药科技有限公司 | Bispecific antibody |
CN110066314A (en) * | 2019-04-01 | 2019-07-30 | 上海药明生物技术有限公司 | A kind of efficient affinity purification technique for improving polymer separation resolution ratio |
CN111018969A (en) * | 2019-12-27 | 2020-04-17 | 上海药明生物技术有限公司 | Method for purifying bispecific antibody by light chain select combined chromatography |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN116655726A (en) * | 2023-07-31 | 2023-08-29 | 上海澳斯康生物制药有限公司 | Antibody purification method based on ion exchange chromatography |
CN116655726B (en) * | 2023-07-31 | 2023-11-14 | 上海澳斯康生物制药有限公司 | Antibody purification method based on ion exchange chromatography |
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