CN114534813A - Trace liquid distribution device - Google Patents
Trace liquid distribution device Download PDFInfo
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- CN114534813A CN114534813A CN202210302382.8A CN202210302382A CN114534813A CN 114534813 A CN114534813 A CN 114534813A CN 202210302382 A CN202210302382 A CN 202210302382A CN 114534813 A CN114534813 A CN 114534813A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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Abstract
The invention discloses a trace liquid distribution device, which comprises a reaction tank and a pressure head part, wherein the reaction tank is provided with a liquid inlet and a liquid outlet; the reaction tank is internally provided with at least one sample adding cavity and a micropore array area, and the micropore array area is provided with an array area platform surface and a plurality of micropores arranged on the array area platform surface; the surface of the pressure head part is provided with a sealing surface and a boss protruding out of the sealing surface, the boss can be filled in the sample adding cavity so that liquid in the sample adding cavity moves into the micropores, and the sealing surface can be pressed with the array area platform surface of the micropore array area to seal the micropores. According to the micro-liquid distribution device provided by the invention, the arrangement of the boss and the sealing surface is utilized to realize the distribution and sealing of liquid, the introduction of oil phase or other reagents is avoided, the operation is convenient and fast, and the stability is high.
Description
Technical Field
The invention relates to the field of in-vitro diagnosis, in particular to a micro-liquid distribution device.
Background
When quantitative detection of nucleic acid, protein and the like is carried out on trace liquid, a conventional detection means is to carry out traditional PCR or real-time fluorescence quantitative PCR, and one of the core requirements is to disperse a sample or a reaction reagent into a large number of uniform and stable tiny droplets.
In the prior art, a water-in-oil droplet generation method and a micropore array droplet generation method are generally used.
The water-in-oil droplet generation method adopts a cross-shaped cross flow channel, utilizes fluid shearing force, cuts off by an oil phase and wraps a water phase reagent to form water-in-oil droplets, and can disperse a reaction sample to form droplets with target quantity and target volume in reaction time; the microwell array droplet formation method requires the pre-formation of an array of microwells of a specific size and number, with the reagent to be dispensed into the array region, the liquid filling each microwell, followed by the sealing of the microwell with another liquid medium (e.g., oil) or a non-liquid medium (e.g., a membrane), i.e., the formation of individual microdroplets.
When micro-amount liquid (mostly aqueous phase in the field of in vitro diagnosis) is uniformly distributed and sealed and isolated from each other, if water-in-oil micro-droplets are generated through an oil phase medium, although a higher droplet number can be obtained, the injection speed and proportion of the oil phase and the aqueous phase need to be accurately controlled, the requirement on system stability is higher, otherwise, the generated droplets have the problem of poor consistency, in addition, the generated droplets can have the problems of cracking and the like, and the storage stability is relatively poor; when the oil phase medium is used for realizing the separation and sealing of the microcavity array, the thickness of an oil film and the injection angle and speed of the oil phase need to be specially set, otherwise, the problems that the water phase cannot be completely removed, or the reagent in the microcavity is flushed out and the like can occur; this results in a complicated structure and operation, and requires a high manipulation technique.
When a non-liquid medium is used for separating and sealing the microcavity array, a thin film for sealing needs to be in full contact with a plane between the microcavity array in a rolling mode, and the squeezing of redundant liquid and the separation of the microcavity array are completed; although the mode avoids the introduction of oil phase, the system stability is relatively high, in the scheme in the prior art, the rolling operation needs to be carried out on a machine, all microcavity arrays can be sealed, and when the sample quantity to be tested is insufficient, the waste of testing resources can be caused; in addition, the use of a film as the sealing structure has a certain risk that, for example, the operator may touch the film by mistake, which may cause the failure of some micro-cavities.
Therefore, how to improve the stability of the micro-liquid dispensing device is a technical problem that needs to be solved by those skilled in the art.
Disclosure of Invention
The invention aims to provide a micro liquid distribution device which can effectively improve the stability of the device and reduce waste.
In order to achieve the purpose, the invention provides the following technical scheme:
a micro-amount liquid distribution device is provided,
comprises a reaction tank and a pressure head part;
the reaction tank is internally provided with at least one sample adding cavity and a micropore array area, and the micropore array area is provided with an array area platform surface and a plurality of micropores arranged on the array area platform surface;
the surface of the pressure head part is provided with a sealing surface and a boss protruding out of the sealing surface, the boss can be filled in the sample adding cavity so that liquid in the sample adding cavity moves into the micropores, and the sealing surface can be pressed with the array area platform surface of the micropore array area to seal the micropores.
Preferably, the surface of the microwell and the position on the array region platform surface, which is close to the microwell, are provided with hydrophilic coatings.
Preferably, the sealing surface of the head portion and the surface of the boss are both provided with a hydrophobic coating.
Preferably, the sealing face of the indenter section is in abutting seal with the array land face.
Preferably, the indenter section is a high molecular polymer elastic indenter section.
Preferably, the reaction tank is a transparent reaction tank.
Preferably, the cross-sectional area of the boss and the sample application cavity gradually increases from one end close to the sample application cavity to one end far away from the sample application cavity.
Preferably, the number of the reaction tanks is multiple, the number of the pressure head parts is the same as that of the reaction tanks, and each pressure head part is independently controlled.
Preferably, the reaction cell is further provided with an overflow area, and the volume of the sample adding cavity is larger than the sum of the volumes of the micropores and smaller than the difference between the volume of the reaction cell and the volume of the pressure head part.
Preferably, the height of the overflow area is lower than the surface height of the microwell array region.
Preferably, the array area platform surface of the micropore array area is provided with a diversion trench for liquid to flow into the overflow area.
Preferably, the sample-adding cavity is located at the middle position of the bottom of the reaction cell, the micropore array area is located at the periphery of the sample-adding cavity, and the overflow area is located outside the micropore array area; the micropore array area and the overflow area are both distributed annularly;
or, the overflow area is positioned at the middle position of the bottom of the reaction cell, the micropore array area is positioned at the periphery of the overflow area, and the sample adding cavity is positioned outside the micropore array area; the micropore array area and the sample adding cavity are both distributed annularly;
or the sample adding cavity is positioned in the middle of the bottom of the reaction cell, and the sample adding cavity and the micropore array area are both in strip distribution; the micropore array area is arranged on at least one side of the sample adding cavity, and the overflow area is arranged on the outer side of the micropore array area.
The invention provides a micro-liquid distribution device, which comprises a reaction tank and a pressure head part; the reaction tank is internally provided with at least one sample adding cavity and a micropore array area, and the micropore array area is provided with an array area platform surface and a plurality of micropores arranged on the array area platform surface; the surface of the pressure head part is provided with a sealing surface and a boss protruding out of the sealing surface, the boss can be filled in the sample adding cavity so that liquid in the sample adding cavity moves into the micropores, and the sealing surface can be pressed with the array area platform surface of the micropore array area to seal the micropores. According to the micro-liquid distribution device provided by the invention, the arrangement of the boss and the sealing surface is utilized to realize the distribution and sealing of liquid, the introduction of oil phase or other reagents is avoided, the operation is convenient and fast, and the stability is high.
In a preferred embodiment, the surface of the microwell and the surface of the array region stage adjacent to the microwell are provided with a hydrophilic coating. According to the arrangement, through the arrangement of the hydrophilic coating, the liquid flowing out of the sample adding cavity can be ensured to be firstly filled to the positions of the micropores, the liquid is ensured to be preferentially filled into the micropores, and then the distribution precision of the liquid is ensured.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of one embodiment of a reaction tank in a micro-liquid dispensing apparatus according to the present invention;
FIG. 2 is a cross-sectional view of the bottom of the reaction cell shown in FIG. 1;
FIG. 3 is a schematic view of a microwell of the reaction cell of FIG. 1;
FIG. 4 is a schematic structural view of one embodiment of a pressing head portion of a micro-fluid dispensing device according to the present invention;
FIG. 5 is a schematic view showing the process of matching the middle pressure head part with the bottom of the reaction well in the micro-fluid dispensing device of the present invention;
FIG. 6 is a schematic structural view of a sealing surface of a pressing head part of the micro-fluid dispensing device of the present invention engaging with a platform surface of an array region in a reaction well;
FIG. 7 is a schematic structural diagram of a micro-fluid dispensing device according to the present invention, wherein a sealing surface of a pressing head portion is in interference fit with a platform surface of an array region in a reaction chamber;
FIG. 8 is a schematic view of the bottom surface of a reaction well in another embodiment of the micro-fluid dispensing apparatus of the present invention;
FIG. 9 is a schematic sectional view taken along line A-A in the reaction cell shown in FIG. 8;
FIG. 10 is a schematic view of the bottom surface of a reaction well in a third embodiment of the micro-fluid dispensing device of the present invention;
FIG. 11 is a schematic sectional view of B-B in the reaction cell shown in FIG. 10;
wherein: 1-a reaction tank; 2-sample application cavity; 3-a microwell array region; 3-1-microwell; 4-array area platform surface; 5-an overflow area; 6-side wall; 7-a head portion; 7-1-boss.
Detailed Description
The core of the invention is to provide a micro-liquid distribution device which can effectively avoid the introduction of oil phase or other reagents, simplify the operation process and improve the stability.
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 11, fig. 1 is a schematic structural diagram of a reaction cell in a micro-liquid distribution device according to an embodiment of the present invention; FIG. 2 is a cross-sectional view of the bottom of the reaction cell shown in FIG. 1;
FIG. 3 is a schematic view of a microwell of the reaction cell of FIG. 1; FIG. 4 is a schematic structural view of one embodiment of a pressing head portion of a micro-fluid dispensing device according to the present invention; FIG. 5 is a schematic view showing the process of matching the middle pressure head part with the bottom of the reaction well in the micro-fluid dispensing device of the present invention; FIG. 6 is a schematic structural view of a sealing surface of a pressing head part of the micro-fluid dispensing device of the present invention engaging with a platform surface of an array region in a reaction well; FIG. 7 is a schematic structural diagram of a micro-fluid dispensing device according to the present invention, wherein a sealing surface of a pressing head portion is in interference fit with a platform surface of an array region in a reaction chamber; FIG. 8 is a schematic view of the bottom surface of a reaction well in another embodiment of the micro-fluid dispensing apparatus of the present invention; FIG. 9 is a schematic sectional view taken along line A-A in the reaction cell shown in FIG. 8; FIG. 10 is a schematic view of the bottom surface of a reaction well in a third embodiment of the micro-fluid dispensing device of the present invention; FIG. 11 is a schematic sectional view of B-B in the reaction cell shown in FIG. 10.
In this embodiment, the micro-volume liquid dispensing apparatus includes the reaction cell 1 and the head portion 7, and can be applied to the field of quantitative detection of nucleic acids and proteins in a micro-volume liquid.
The reaction cell 1 is internally provided with at least one sample adding cavity 2 and a micropore array area 3, liquid can be added into the sample adding cavity 2, the reaction cell 1 is provided with a side wall 6 and can be limited in position with a pressure head part 7, the moving direction of the pressure head part 7 is ensured not to deviate, and meanwhile, the liquid is prevented from flowing out of the reaction cell 1; further, the micropore array area 3 is provided with an array area platform surface 4 and a plurality of micropores 3-1 arranged on the array area platform surface 4, and liquid flows into the micropores 3-1 from the sample adding cavity 2 to realize the distribution of the liquid; the surface of the pressure head part 7 is provided with a sealing surface and a boss 7-1 protruding out of the sealing surface, the boss 7-1 can be filled into the sample adding cavity 2, so that liquid in the sample adding cavity 2 moves into the micropore 3-1, and the sealing surface can be pressed with the array area platform surface 4 of the micropore array area 3 to seal the micropore 3-1.
According to the micro-liquid distribution device provided by the invention, the arrangement of the boss 7-1 and the sealing surface is utilized to realize the distribution and sealing of liquid, the introduction of oil phase or other reagents is avoided, the operation is convenient and fast, and the stability is high; when the sizes of the micro-wells 3-1 are uniform, the device can realize uniform distribution, sealing and isolation of the liquids in the micro-wells 3-1 from each other.
Specifically, the reaction tank 1 is used for adding a reagent or a sample to be detected, and when the reaction tank is matched with the pressure head part 7, the distribution, the sealing and the mutual isolation of the reagent or the sample to be detected are completed; the reaction tank 1 is a pit structure, and the material can be metal, glass, silicon and other machinable materials, and can also be high molecular polymer, such as polycarbonate with elastic modulus > 0.7, polypropylene, polystyrene, and polymethyl methacrylate.
Further, the micro-wells 3-1 are micro blind holes uniformly arranged at the bottom of the reaction cell 1 for filling with a reagent or a sample to be tested, the structure of the micro-wells 3-1 can be a hemisphere, a cube or other structures, the number and size of the micro-wells 3-1 are set according to the number of micro-droplets to be divided and the required droplet volume, for example, 20000 micro-wells 3-1 are provided, and the volume of each micro-well 3-1 is generally between fL level and μ L level. The volume and aspect ratio of the micro-wells 3-1 are also determined by the corresponding processing capabilities, as known to those skilled in the art, such as machining, injection molding, chemical etching, laser drilling, etc., but the micro-wells 3-1 should be fully filled with reagents or the sample to be tested. Of course, the arrangement of the micro-holes 3-1 may be non-uniform and may be set as required, and is not limited to the embodiment given in the present embodiment.
Furthermore, the array area platform surface 4 is a non-micropore 3-1 part which is positioned between the bottom of the reaction tank 1 and the micropore array area 3 and is a plane; preferably, the minimum distance between the edges of two adjacent micro-holes 3-1 is equal or approximately equal, and the distance may be approximately equal to, greater than or slightly smaller than the diameter of the micro-hole 3-1, so as to ensure the uniform distribution of the micro-holes 3-1 and the stable sealing effect. Of course, the arrangement of the micropores 3-1 may also be irregular and may be set according to the requirement, and is not limited to the solution given in this embodiment.
Further, in order to achieve the above object, in addition to the control of the structure and the aspect ratio, the structure of the microwell 3-1 may be modified accordingly, such as providing a hydrophilic coating on the surface of the microwell 3-1 and the flat surface 4 of the array region near the microwell 3-1. In particular, hydrophilic coatings include, but are not limited to, physical hydrophilic modifications, such as plasma treatment, chemical hydrophilic modifications, such as hydrophilic agent coating. According to the arrangement, through the arrangement of the hydrophilic coating, the liquid flowing out of the sample adding cavity 2 can be ensured to be firstly filled to the positions of the micropores 3-1, namely, the hydrophilic coating plays a role in drainage, the liquid is ensured to be preferentially filled into the micropores 3-1, and then the distribution precision of the liquid is ensured. On the basis of the above embodiments, the sealing surface of the indenter portion 7 and the surface of the boss 7-1 are provided with hydrophobic coatings.
On the basis of the above embodiments, the sealing surface of the indenter portion 7 is in fit sealing with the array area platform surface 4; namely, the sealing surface of the pressure head part 7 is completely attached to the array area platform surface 4, so that the sealing effect of the sealing surface on the micropores 3-1 is realized, and liquid is prevented from flowing out of the micropores 3-1.
In addition to the above embodiments, the indenter portion 7 is a high-molecular polymer elastic indenter portion. Specifically, regarding the arrangement positions of the sealing surface and the boss 7-1, a pressing part is arranged at one end of the pressure head part 7 close to the reaction tank 1, then the sealing surface and the boss 7-1 are arranged on the pressing part, the pressing part is made of metal materials or high polymer materials, and other parts of the pressure head part 7 are not required to be limited, so that the cost is saved. Of course, the sealing surface of the pressing portion can firstly ensure sealing, and the sealing manner can be realized by improving the surface processing precision of the sealing surface besides the interference state of the pressing head portion 7 and the array area platform surface 4 by adopting the elastic pressing head portion, for example, the pressing head portion 7 or the pressing portion is made of metal material, the surface processing precision of the sealing surface is very high, and the surface precision of the micropore array area 3 is also very high, which can be realized in the same way, but is not limited to the manner provided by the embodiment.
In one embodiment, the head portion 7 is adapted to cooperate with the well 1 to dispense, seal, and isolate reagents or samples to be tested. The head portion 7 is a columnar structure, and the material is preferably a high molecular polymer, such as polycarbonate with an elastic modulus > 0.7GPa, polypropylene, polystyrene, polymethyl methacrylate, or rubber or silica gel. The indenter section 7 material itself, or the indenter section 7 after surface treatment, such as teflon coating, has a surface energy close to or larger than the liquid reagent or the sample to be measured, i.e. the liquid does not spread out and wet at the contact surface with the indenter section 7. Its projection in the horizontal direction can cover micropore array district 3 completely, and slightly is less than reaction cell 1 and overflow area 5, simultaneously, when the projection of pressure head part 7 horizontal direction is in a certain specific position in reaction cell 1 completely, on the position that overlaps completely with the projection of application of sample chamber 2 horizontal direction in reaction cell 1 on pressure head part 7, there is the protruding structure that is completely complementary with application of sample chamber 2 shape. When the sample loading device is subjected to the driving force from the outside, acts on the top of the pressure head part 7 along the vertical direction and downwards presses the pressure head part 7, the pressure head part 7 is continuously close to the micropore array area 3 until the boss 7-1 and the sample loading cavity 2 are completely complemented, the plane of the root part of the boss 7-1, namely the bottom plane of the pressure head part 7, namely the sealing surface, is just superposed or slightly interfered with the array area platform surface 4, and the sealing of the micropore 3-1 cavity is finished; the degree of interference is determined by the pressing pressure and the pressing distance to the indenter portion 7 to adjust the sealing strength thereof to the micro-hole 3-1. After the reagent or the sample to be detected is added into the sample adding cavity 2, the pressure head part 7 is pressed into the reaction cell 1 along the vertical direction, the protruding structure at the bottom of the pressure head part 7 gradually enters the sample adding cavity 2, the reagent or the sample to be detected is extruded outwards, the reagent or the sample to be detected flows through the micropore array area 3, and the filling of micropores 3-1 in the sample adding cavity is completed. When the protruding structure at the bottom of the pressure head part 7 completely coincides with the sample adding cavity 2, the driving and distribution of the reagent or the sample to be detected are completed, and simultaneously, the plane at the bottom of the pressure head part 7 coincides with or slightly interferes with the platform surface 4 of the array area, so that the sealing of the micropore 3-1 cavity is completed.
In addition to the above embodiments, the reaction cell 1 is a transparent reaction cell. Specifically, the reaction tank 1 may be a metal reaction tank, a glass reaction tank, a silicon reaction tank or a high molecular polymer reaction tank; the reaction cell 1 is preferably made of transparent, high-molecular hard transparent material or glass to facilitate optical signal detection.
On the basis of the above embodiments, the cross-sectional areas of the boss 7-1 and the sample adding cavity 2 gradually increase from the end close to the sample adding cavity 2 to the end far away from the sample adding cavity 2, so that the boss 7-1 and the sample adding cavity 2 can be conveniently matched to extrude liquid. It should be noted that, the shapes of the boss 7-1 and the sample adding cavity 2 should ensure that the boss 7-1 can smoothly press out the liquid and that excessive friction is not generated during the process of pressing the boss 7-1 into the sample adding cavity 2, and the shapes of the boss 7-1 and the sample adding cavity 2 can be set according to actual needs, and are not limited to the manner provided in this embodiment. Preferably, the shape and size of the boss 7-1 and the sample application cavity 2 are the same or substantially the same, which can ensure that the liquid in the sample application cavity 2 is completely extruded or extruded as much as possible. Certainly, the size of the boss 7-1 can be different from that of the sample adding cavity 2, that is, the size of the boss 7-1 is smaller than that of the sample adding cavity 2, and on the premise that sealing can be guaranteed, the boss 7-1 can extrude the liquid part in the sample adding cavity 2.
In addition to the above embodiments, the reaction cell 1 is provided in plural, the number of the indenter sections 7 is the same as that of the reaction cell 1, and each indenter section 7 is individually controlled. Preferably, each reaction tank 1 is arranged on the same substrate, so that large-scale parallel test can be realized, and meanwhile, the processing and the moving are convenient.
On the basis of the above embodiments, the reaction cell 1 includes a micro-well array region 3, an array region platform surface 4, a sample addition cavity 2, and an overflow region 5; namely, the reaction tank 1 is also provided with an overflow area 5. The volume of the sample adding cavity 2 is larger than the sum of the volumes of the micropores 3-1, and the volume of the extruded liquid is larger than or equal to the sum of the volumes of the micropores 3-1.
Further, the sample adding cavity 2 is located at the bottom of the reaction chamber 1, and is a blind hole or a groove near the micro-well array region 3, the number of which can be 1 or more, and the structure can be a hemisphere, a cube, a V-shaped groove or other structures, preferably, a hemisphere structure. When 1 sample addition cavity 2 is provided, the position is preferably located at the center of the micropore array region 3; when more than 1 sample adding cavity 2 is provided, each sample adding cavity 2 can be independent from each other, or can be communicated with at least one other sample adding cavity 2, and the positions of the sample adding cavities can be uniformly distributed in the micropore array area 3; the total volume of the sample addition chamber 2 is required to be capable of accommodating all reagents to be added or samples to be detected, and is larger than the total volume of all micropores 3-1 in the micropore array area 3. After a user injects a reagent or a sample to be detected into the sample injection cavity 2 through a quantitative sample injection device such as a pipettor, the liquid level of the liquid is not higher than the top of the sample injection cavity 2.
In addition to the above embodiments, the height of the overflow area 5 is lower than the surface height of the microwell array region 3. Specifically, the overflow area 5 is located at the bottom of the reaction tank 1, is arranged at the outer edge of the micropore array area 3, and is used for overflowing and storing redundant reagents or liquid after the micropores 3-1 are filled, and the overflow area 5 can be of a groove-shaped structure.
Preferably, the array platform surface 4 of the micropore array area 3 is provided with a diversion trench for liquid flowing into the overflow area 5. The overflow district 5 and micropore array district 3 are through the guiding gutter of independent setting promptly, of course, the guiding gutter does not set up and does, and overflow district 5 directly links to each other with array district platform face 4, and the highest position of overflow district 5 is not higher than array district platform face 4's height, and the liquid that flows to array district platform face 4 edge that makes flows to overflow district 5 under the effect of gravity.
On the basis of the above embodiments, as shown in FIG. 1, the sample addition chamber 2 is located at the middle position of the bottom of the reaction cell 1, the micro well array region 3 is located at the outer peripheral part of the sample addition chamber 2, and the overflow region 5 is located at the outer part of the micro well array region 3; the micropore array area 3 and the overflow area 5 are both distributed annularly; according to the arrangement, the sample adding cavity 2 is positioned in the middle of the bottom of the reaction tank 1, and the boss 7-1 is also positioned in the middle of the sealing surface, so that the boss 7-1 can be conveniently processed and corresponds to the boss 7-1 in position.
Of course, the position of the sample-adding cavity 2 can also be set at the bottom edge of the reaction cell 1, as shown in fig. 8 and 9, the overflow area 5 is located at the bottom middle position of the reaction cell 1, the micro-pore array area 3 is located at the outer periphery of the overflow area 5, and the sample-adding cavity 2 is located at the outer part of the micro-pore array area 3; the micropore array area 3 and the sample adding cavity 2 are both distributed annularly; moreover, the cross section of the sample adding cavity 2 is preferably V-shaped, which is convenient for liquid extrusion, and of course, the cross section of the sample adding cavity 2 can be in other shapes, such as U-shaped. It should be noted that, when the position of the sample application chamber 2 is set at the bottom edge of the reaction cell 1, the position of the boss 7-1 should be correspondingly set at the edge position of the sealing surface of the pressure head part 7 so as to correspond to the position of the sample application chamber 2.
Of course, as shown in fig. 10 and fig. 11, the sample application cavity 2 and the micro-well array region 3 can also be distributed in a strip shape, that is, the sample application cavity 2 is located at the middle position of the bottom of the reaction cell 1, and the sample application cavity 2 and the micro-well array region 3 are both distributed in a strip shape; the micropore array regions 3 are arranged on at least one side of the sample adding cavity 2, preferably, the micropore array regions 3 are symmetrically arranged on two sides of the sample adding cavity 2 at equal intervals to ensure uniform distribution, and the overflow regions 5 are arranged on the outer sides of the micropore array regions 3; similarly, the cross section of the sample adding cavity 2 is preferably V-shaped, which is convenient for liquid extrusion, and of course, the cross section of the sample adding cavity 2 can be other shapes, such as U-shaped.
Specifically, as shown in fig. 5, the pressure head part 7 gradually approaches the bottom of the reaction cell 1 along the vertical direction, the boss 7-1 of the pressure head part 7 gradually coincides with the sample addition cavity 2 in the reaction cell 1, and when the two are completely coincident and complementary, or slightly interfere with each other, the sealing surface of the pressure head part 7 seals the microwell 3-1 in the microwell array region 3; as shown in fig. 6, the sealing surface of the pressure head part 7 is just coincident with the array area platform surface 4, and no interaction force exists between the two; as shown in fig. 7, the "interference" state refers to a compression state formed by the fact that after the sealing surface of the pressure head portion 7 and the array area platform surface 4 are overlapped, the pressure head portion 7 continues to move towards the array area platform surface 4 under the action of an external driving force, and an interaction force exists between the two surfaces.
Compared with a water-in-oil micro-droplet generation mode in the prior art, the micro-liquid distribution device provided by the embodiment avoids introduction of oil phase or other reagents, reduces operation steps and improves system stability while realizing uniform distribution and stable storage of samples; compared with the existing oil-free sealing scheme, each reaction tank 1 and the micropore array area 3 in the reaction tank and the pressure head part 7 in the device are in one-to-one correspondence, large-scale parallel test can be carried out by repeatedly arranging arrays on the structure, meanwhile, each unit can be independently sealed and independently used, and once sealing action is carried out, all reaction chambers in the same array are sealed, so that when the sample to be tested is insufficient, the waste of test resources is caused; compared with the existing oil-free sealing scheme, the sealing pressure head part 7 in the device is only arranged in the reaction tank 1 before reaction/test is prepared, sealing action is executed, and the problem that in other schemes, a film structure can be directly touched due to misoperation, and then a test hole is failed is solved.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The micro-fluid dispensing device provided by the present invention is described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, it is possible to make various improvements and modifications to the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (12)
1. A micro-quantity liquid dispensing device, characterized in that,
comprises a reaction tank (1) and a pressure head part (7);
at least one sample adding cavity (2) and a micropore array area (3) are arranged in the reaction tank (1), the micropore array area (3) is provided with an array area platform surface (4) and a plurality of micropores (3-1) formed on the array area platform surface (4);
the surface of the pressure head part (7) is provided with a sealing surface and a boss (7-1) protruding out of the sealing surface, the boss (7-1) can be filled into the sample adding cavity (2) so as to enable liquid in the sample adding cavity (2) to move into the micropore (3-1), and the sealing surface can be pressed with the array area platform surface (4) of the micropore array area (3) to seal the micropore (3-1).
2. The micro liquid dispensing device according to claim 1, wherein the surface of the micro well (3-1) and the array area stage surface (4) are provided with a hydrophilic coating layer at a position close to the micro well (3-1).
3. The micro liquid dispensing device as claimed in claim 2, wherein the sealing surface of the head portion (7) and the surface of the boss (7-1) are provided with a hydrophobic coating.
4. The microfluidic dispensing device according to claim 1, wherein the sealing surface of the head section (7) is in flush sealing with the array area platform surface (4).
5. The micro-fluid dispensing device as claimed in claim 1, wherein the head portion (7) is a high molecular polymer elastic head portion.
6. The micro liquid dispensing device according to claim 1, wherein the reaction cell (1) is a transparent reaction cell.
7. The micro liquid dispensing device according to claim 1, wherein the cross-sectional area of the projection (7-1) and the sample application chamber (2) gradually increases from an end close to the sample application chamber (2) to an end far from the sample application chamber (2).
8. The micro-liquid dispensing device according to any one of claims 1 to 7, wherein the reaction cell (1) is plural, the number of the head parts (7) is the same as that of the reaction cell (1), and each of the head parts (7) is individually controlled.
9. The micro-fluid dispensing device according to any one of claims 1 to 7, wherein the reaction cell (1) is further provided with an overflow area (5), and the volume of the sample addition chamber (2) is larger than the sum of the volumes of the micro wells (3-1) and smaller than the difference between the volume of the reaction cell (1) and the volume of the head portion (7).
10. The micro liquid dispensing device according to claim 9, wherein the overflow area (5) has a height lower than a surface height of the micro well array area (3).
11. The micro liquid dispensing device according to claim 9, wherein the array region land (4) of the micro well array region (3) is provided with a flow guide groove for flowing the liquid into the overflow region (5).
12. The micro fluid dispensing device according to any one of claims 1 to 7, wherein the sample application chamber (2) is located at a bottom middle position of the reaction cell (1), the micro well array region (3) is located at a periphery of the sample application chamber (2), and the overflow region (5) is located at an outer portion of the micro well array region (3); the micropore array area (3) and the overflow area (5) are both distributed annularly;
or, the overflow area (5) is positioned at the middle position of the bottom of the reaction cell (1), the micropore array area (3) is positioned at the periphery of the overflow area (5), and the sample adding cavity (2) is positioned outside the micropore array area (3); the micropore array area (3) and the sample adding cavity (2) are distributed annularly; or the sample adding cavity (2) is positioned in the middle position of the bottom of the reaction cell (1), and the sample adding cavity (2) and the micropore array area (3) are both in strip distribution; the micropore array area (3) is arranged on at least one side of the sample adding cavity (2), and the overflow area (5) is arranged on the outer side of the micropore array area (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210302382.8A CN114534813A (en) | 2022-03-25 | 2022-03-25 | Trace liquid distribution device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210302382.8A CN114534813A (en) | 2022-03-25 | 2022-03-25 | Trace liquid distribution device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114534813A true CN114534813A (en) | 2022-05-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210302382.8A Pending CN114534813A (en) | 2022-03-25 | 2022-03-25 | Trace liquid distribution device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114534813A (en) |
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2022
- 2022-03-25 CN CN202210302382.8A patent/CN114534813A/en active Pending
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