CN114532362B - Microbial agent for preventing and treating lepidoptera pests of fruits and vegetables as well as preparation method and application of microbial agent - Google Patents
Microbial agent for preventing and treating lepidoptera pests of fruits and vegetables as well as preparation method and application of microbial agent Download PDFInfo
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- CN114532362B CN114532362B CN202210185994.3A CN202210185994A CN114532362B CN 114532362 B CN114532362 B CN 114532362B CN 202210185994 A CN202210185994 A CN 202210185994A CN 114532362 B CN114532362 B CN 114532362B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a microbial agent for preventing and treating lepidoptera pests of fruits and vegetables, which comprises bacterial powder and titanium-doped cryptopotash manganese; the fungus powder comprises bacillus firmus powder, verticillium pachycorum powder and beauveria bassiana powder; the titanium-doped crypto-kalium manganese is prepared by calcining octahedral manganese oxide molecular sieve and tetraisopropoxytitanium by an impregnation method; the mass ratio of the bacterial powder to the titanium-doped crypto-kalium manganese is 20-10; the mass ratio of the bacillus firmus powder to the verticillium dahliae powder to the beauveria bassiana powder is 2-4:3-7:5-10; the molar amount of Ti in the Ti-doped cryptokalium manganese with the molar ratio of Ti/Mn is 0.20. In the microbial agent, bacillus firmus powder, verticillium pachycephalum powder and beauveria bassiana powder have synergistic interaction in the prevention and treatment of lepidoptera pests such as plutella xylostella and the like, have higher prevention and treatment effects, and the activity, the ultraviolet resistance and the storage stability of the agent are enhanced by adding self-made titanium doped cryptokalium manganese.
Description
Technical Field
The invention relates to the technical field of microbial agents, in particular to a microbial agent for preventing and treating lepidoptera pests of fruits and vegetables, and a preparation method and application thereof.
Background
In recent years, with the research on the side effect of chemical pesticides on crops and the resistance of pests and germs, people tend to use microbial agents for resisting the diseases and pests of the crops more and more, and the microbial agents can slow down the generation of drug resistance, are safe to the crops and human bodies, and are beneficial to good ecological construction. However, the existing microbial bacteria have many problems in biological control application, for example, volatile activity of the microbial bacteria is easily caused by field environment change, unstable storage environment and the like, so that the pesticide effect is unstable, the pesticide effect period is short and the like, and the popularization and the use of the microbial agent are greatly limited.
Disclosure of Invention
In view of the above situation, the present invention aims to provide a microbial agent for controlling lepidopteran pests of fruits and vegetables, wherein the microbial agent has high biological activity and stability.
The technical scheme for solving the problem is as follows:
a microbial agent for preventing and treating lepidoptera pests of fruits and vegetables comprises bacterial powder and titanium-doped cryptopotash manganese; the fungus powder comprises bacillus firmus powder, verticillium pachycorum powder and beauveria bassiana powder; the titanium-doped cryptokalium manganese is prepared by calcining octahedral manganese oxide molecular sieve and titanium tetraisopropoxide by an impregnation method.
Further, the mass ratio of the bacterial powder to the titanium-doped crypto-kalium manganese is 20-10.
Furthermore, the mass ratio of the bacillus firmus powder to the verticillium pachymenianum powder to the beauveria bassiana powder is 2-4:3-7:5-10.
Further, the molar amount of Ti in the Ti-doped cryptopotash manganese in the molar ratio of Ti/Mn is 0.20.
Further, the titanium-doped cryptopotassium manganese is prepared by the following method:
(1) 0.4mol/L of KMnO 4 The solution was added to 1.75mol/L MnSO 4 Solution and 68% HNO 3 Stirring the mixture of the solution at the speed of 1200-1500r/min, refluxing for 24h at 100 ℃, cooling to room temperature to obtain black precipitate, filtering, washing the black precipitate with deionized water until the pH value is neutral, and drying for 24h at 120 ℃ to obtain octahedral manganese oxide;
(2) Adding titanium tetraisopropoxide into a toluene solution, adding the octahedral manganese oxide, stirring for 20min, taking out the octahedral manganese oxide molecular sieve powder impregnated with the titanium tetraisopropoxide, calcining for 3h at 500 ℃, cooling to room temperature, grinding and sieving to 154 mu m to obtain the titanium-doped cryptopotash manganese.
Further, in the step (1), KMnO is adopted 4 Solution of MnSO 4 Solution of HNO 3 The mass-to-volume ratio of the solution was 200mL.
Further, in the step (2), the mass-volume ratio of the titanium tetraisopropoxide to the toluene solution to the octahedral manganese oxide is 2.1g; the concentration of the toluene solution was 5mol/L.
Further, the microbial agent is prepared by the following method: and taking the bacterial powder and the titanium-doped cryptokaline manganese according to the proportion, adding the bacterial powder and the titanium-doped cryptokaline manganese into deionized water with the mass 5 times of that of the bacterial powder, fully mixing the mixture, then placing the mixture in a constant-temperature shaking table with the temperature of 28 ℃ at 250r/min for 1h, and then placing the mixture in a constant-temperature shaking table with the temperature of 32 ℃ for 1h to obtain the microbial agent.
Furthermore, the microbial agent is used for preventing and controlling diamondback moths.
Due to the adoption of the technical scheme, compared with the prior art, the invention has the following advantages:
in the microbial agent prepared by the invention, the bacillus firmus powder, the verticillium pachycorum powder and the beauveria bassiana powder have synergistic effect in the prevention and treatment of lepidoptera pests such as plutella xylostella and the like, and have higher prevention and treatment effect.
Detailed Description
The technical solution of the present invention is described in detail with reference to the specific embodiments, but it should be emphasized that the present invention is not limited to the specific embodiments; the microbial species used in the examples below can be isolated by themselves or obtained by purchase; the starting materials described in the following examples can be prepared or purchased by conventional methods without specific reference.
Example 1
A preparation method of titanium doped cryptopotassium manganese is provided:
(1) 0.4mol/L of KMnO 4 200mL of the solution was added to 1.75mol/L MnSO 4 Solution 70mL and 68% HNO 3 Stirring the solution 7mL in the mixture at the speed of 1200-1500r/min, refluxing for 24h at 100 ℃, cooling to room temperature to obtain black precipitate, filtering, washing the black precipitate with deionized water until the pH value is neutral, and drying for 24h at 120 ℃ to obtain octahedral manganese oxide;
(2) Adding 2.1g of titanium tetraisopropoxide into 20mL of 5mol/L toluene solution, adding 5.2g of octahedral manganese oxide, stirring for 20min, taking out octahedral manganese oxide molecular sieve powder impregnated with titanium tetraisopropoxide, calcining for 3h at 500 ℃, cooling to room temperature, grinding and sieving to 154 mu m to obtain the titanium-doped cryptokalium manganese. The molar quantity of Ti in the molar ratio of Ti/Mn in the obtained titanium-doped cryptokalium manganese is 0.20.
Example 2
Providing a microbial agent for preventing and controlling lepidoptera pests of fruits and vegetables, wherein the microbial agent comprises bacterial powder and titanium-doped cryptopotash manganese; the fungus powder comprises bacillus firmus powder, verticillium pachycorum powder and beauveria powder, and the mass ratio of the bacillus firmus powder to the beauveria bassiana powder is 2; the mass ratio of the bacterial powder to the titanium-doped crypto-kalium manganese is 20.
Example 3
Providing a microbial agent for preventing and controlling lepidoptera pests of fruits and vegetables, wherein the microbial agent comprises bacterial powder and titanium-doped cryptopotash manganese; the fungus powder comprises bacillus firmus powder, verticillium pachydenum powder and beauveria bassiana powder, and the mass ratio of the bacillus firmus powder to the beauveria bassiana powder is 3; the mass ratio of the bacterial powder to the titanium-doped crypto-kalium manganese is 15.
Example 4
The microbial agent for preventing and treating lepidoptera pests of fruits and vegetables comprises bacterial powder and titanium-doped cryptomelane; the fungus powder comprises bacillus firmus powder, verticillium pachydenum powder and beauveria bassiana powder, and the mass ratio of the fungus powder is 4; the mass ratio of the bacterial powder to the titanium-doped crypto-potassium manganese is 10.
Comparative example 1
The same as in example 3, except that the powder of Bacillus firmus was replaced with the same amount of powder of Verticillium pachyrhizi.
Comparative example 2
The only difference was that in example 3, verticillium pachymenianum powder was replaced with an equal amount of beauveria bassiana powder.
Comparative example 3
The same as example 3, except that the powder of Beauveria bassiana was replaced with an equal amount of the powder of Bacillus firmus.
Comparative example 4
The only difference was that titanium doped cryptokalium manganese was removed as in example 3.
Comparative example 5
The same as example 3, except that titanium doped cryptokalium manganese was replaced by an equal amount of manganese dioxide.
Example 5
Provides a preparation method of a microbial agent for preventing and controlling fruit and vegetable lepidoptera pests, which comprises the following steps: taking the bacterial powder and the titanium-doped crypto-kalium manganese according to the proportion, adding the bacterial powder and the titanium-doped crypto-kalium manganese into 5 times of deionized water by mass, fully mixing the mixture, then placing the mixture in a constant temperature shaking table at the temperature of 28 ℃ for 1h at the speed of 250r/min, and then placing the mixture in a constant temperature shaking table at the temperature of 32 ℃ for 1h to obtain the microbial agent.
Test example 1
(1) Absolute lethal experiment of microbial agents:
subject: starvation treatment is carried out for 1h, and then the plutella xylostella of 4 days old is subjected to starvation treatment;
experimental products: the microbial agents prepared in examples 2-4 of the present invention and comparative examples 1-5.
(2) Ultraviolet resistance test of microbial inoculum:
subject: starvation treatment is carried out for 1h, and then the plutella xylostella of 4 days old is subjected to starvation treatment;
experimental products: the microbial inoculum prepared in the examples 2-4 and the comparative examples 4-5 of the invention is treated by the following steps: irradiating under ultraviolet lamp (315-280 nm, height 25 cm) for 2 hr, and standing in room at room temperature for 7d.
(3) Storage-resistant capability test of microbial agent:
subject: starvation treatment of 4-day-old diamondback moths after 1 h;
experimental products: the microbial inoculum prepared in the embodiments 2-4 and the comparative examples 4-5 of the invention is treated by the following steps: the mixture was left indoors at 25 ℃ for 45 days.
The experimental method comprises the following steps: (1) Mixing 18g of casein, 20g of wheat germ powder, 10g of cane sugar, 1.2g of mineral salt, 0.05g of potassium sorbate, 1g of cellulose, 0.31g of methyl benzoate, 1.5g of vitamin C,0.6g of aureomycin and 1.1g of propyl gallate, and adding 100mL of distilled water to obtain a mixture; adding paraffin into 200mL of distilled water, heating to completely melt the paraffin, cooling to 60 ℃, mixing with the mixture, rapidly stirring uniformly to obtain a plutella xylostella feed, and keeping the temperature at 60 ℃; (2) Adding 1g of the test substance into 120mL of phosphate buffer solution, uniformly mixing, then uniformly mixing 4mL of the test substance with 26mL of feed, solidifying into blocks, then adding 15 living 4-day-old plutella xylostella subjected to starvation treatment for 1h, culturing at 27 ℃ for 72h, arranging another group of control groups, and replacing the test substance with the same amount of clear water by the control groups; (3) The lethality of the diamondback moth is observed and counted, and the lethality% = (the number of the diamondback moths in the control group-the number of the diamondback moths in the experimental group)/the number of the diamondback moths in the control group) × 100, and the results are shown in table 1:
as can be seen from table 1, compared with comparative examples 1 to 3, the microbial agent prepared by the invention combines bacillus firmus powder, verticillium pachycete powder and beauveria bassiana powder for use, has the highest absolute lethality to plutella xylostella, compared with comparative examples 4 to 5, titanium-doped cryptopotash manganese has an improved absolute lethality to plutella xylostella, and the combination of titanium-doped cryptopotash manganese with bacillus firmus powder, verticillium pachycete powder and beauveria bassiana powder is beneficial to improving the ultraviolet resistance and storage capacity of the bacterial powder in terms of the lethality after ultraviolet treatment and the lethality after standing at normal temperature.
While the invention has been described in further detail with reference to specific embodiments thereof, it is not intended that the invention be limited to the specific embodiments thereof; for those skilled in the art to which the present invention pertains and related arts, the extension, operation method, and data replacement shall fall within the scope of the present invention based on the technical idea of the present invention.
Claims (5)
1. A microbial agent for preventing and controlling lepidoptera pests of fruits and vegetables is characterized by comprising bacterial powder and titanium-doped crypto-kalium manganese; the fungus powder comprises bacillus firmus powder, verticillium pachycorum powder and beauveria bassiana powder; the titanium-doped cryptokalium manganese is prepared by calcining octahedral manganese oxide molecular sieve and titanium tetraisopropoxide by an impregnation method; the mass ratio of the bacterial powder to the titanium-doped crypto-kalium manganese is 20-10; the mass ratio of the bacillus firmus powder to the verticillium pachymenianum powder to the beauveria bassiana powder is 2-4:3-7:5-10; the molar weight of Ti in the Ti-doped cryptopotash manganese with the molar ratio of Ti/Mn is 0.20;
the titanium-doped crypto-potassium manganese is prepared by the following method:
(1) 0.4mol/L of KMnO 4 The solution was added to 1.75mol/L MnSO 4 Solution and 68% HNO 3 Stirring the solution mixture at 1200-1500r/min, refluxing at 100 deg.C for 24 hr, cooling to room temperature to obtain black precipitate, filtering, washing with deionized water until the pH is neutral, and drying at 120 deg.C for 24 hr to obtain octahedral manganese oxide;
(2) Adding titanium tetraisopropoxide into a toluene solution, adding the octahedral manganese oxide, stirring for 20min, taking out the octahedral manganese oxide molecular sieve powder impregnated with the titanium tetraisopropoxide, calcining for 3h at 500 ℃, cooling to room temperature, grinding and sieving to 154 mu m to obtain the titanium-doped cryptopotash manganese.
2. The microbial agent for controlling lepidopteran pests of fruits and vegetables according to claim 1, wherein KMnO is used in step (1) 4 Solution of MnSO 4 Solution of HNO 3 The mass-to-volume ratio of the solution was 200mL.
3. The microbial agent for controlling lepidopteran pests on fruits and vegetables according to claim 2, wherein in the step (2), the mass-to-volume ratio of the titanium tetraisopropoxide to the toluene solution to the octahedral manganese oxide is 2.1g; the concentration of the toluene solution is 5mol/L.
4. The microbial agent for controlling lepidopteran pests on fruits and vegetables according to any one of claims 1 to 3, wherein the microbial agent is prepared by the following method: and taking the bacterial powder and the titanium-doped cryptokal manganese according to the proportion, adding the bacterial powder and the titanium-doped cryptokal manganese into deionized water with the mass 5 times that of the deionized water, fully and uniformly mixing, then placing the mixture in a constant-temperature shaking table with the speed of 250r/min and the temperature of 28 ℃ for 1h, and then placing the mixture in a constant-temperature shaking table with the temperature of 32 ℃ for 1h to obtain the microbial agent.
5. The microbial agent for controlling lepidoptera pests on fruits and vegetables according to claim 4, wherein the microbial agent is used for controlling plutella xylostella.
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