CN114527265A

CN114527265A - Spectral detection method for urine components

Info

Publication number: CN114527265A
Application number: CN202111666382.8A
Authority: CN
Inventors: 闵浩迪
Original assignee: Youtai Technology Taizhou Co ltd
Current assignee: Youtai Technology Taizhou Co ltd
Priority date: 2021-12-31
Filing date: 2021-12-31
Publication date: 2022-05-24

Abstract

The invention provides a spectral detection method of urine components, belongs to the field of biological detection, and solves the problem that a conventional spectral detector in the prior art is difficult to extract and store relevant information of a urine sample. The spectral detection method of the urine components comprises the following steps: injecting urine into the sample detection chamber; controlling light of a background light source to enter the sample detection chamber through the cavity wall of the sample detection chamber; collecting spectral information of the urine through the sample detection chamber; and carrying out urine detection according to the acquired spectral information of the urine. The spectral detection method for urine components provided by the invention is easier to extract and store the relevant information of the urine sample, and the detection process is simple.

Description

Spectral detection method for urine components

Technical Field

The invention belongs to the technical field of biological detection, and particularly relates to a spectral detection method for urine components.

Background

The urine examination includes routine analysis of urine, detection of formed components in urine (such as urine red blood cells, leukocytes, etc.), quantitative determination of protein components, and measurement of urease. Urine tests have important values for clinical diagnosis, judgment of curative effect and prognosis.

At present, a spectrometer is often adopted to directly detect urine, an operator puts a urine sample into a detection instrument and manually observes under the spectrometer, the instrument can only be observed on the spot, relevant information of the urine sample cannot be extracted, and initial sample information is difficult to store.

Disclosure of Invention

In view of this, the present invention provides a method for detecting a spectrum of urine components, so as to solve the problem that the conventional spectrum detector in the prior art is difficult to extract and store relevant information of a urine sample.

The technical scheme adopted by the invention is as follows:

the invention provides a spectral detection method of urine components, which comprises the following steps:

injecting urine into the sample detection chamber;

controlling light of a background light source to enter the sample detection chamber through the cavity wall of the sample detection chamber;

collecting spectral information of the urine through the sample detection chamber;

and carrying out urine detection according to the acquired spectral information of the urine.

As a preferable aspect of the above-described method for spectroscopic detection of a urine component, the temperature of urine in the sample detection chamber is adjusted after the urine is injected into the sample detection chamber.

As a preferable scheme of the above method for detecting urine components by spectrum, before the light of the background light source passes through the cavity wall of the sample detection chamber, the light of the background light source is filtered to obtain light within a preset wavelength range.

As a preferable mode of the above-mentioned method for detecting a spectrum of a urine component, light transmitted through the sample detection chamber is filtered before collecting spectrum information of the urine.

As a preferable scheme of the above method for detecting the urine component by spectrum, before controlling the light of the background light source to penetrate through the wall of the sample detection chamber and enter the sample detection chamber, the method includes: a spectroscopic detection reagent is injected into the sample detection chamber.

As a preferable aspect of the above method for detecting a spectrum of a urine component, the spectrum detection includes: at least one of Fourier infrared spectroscopy detection, Raman spectroscopy detection, fluorescence spectroscopy detection, and ultraviolet spectroscopy detection.

As a preferable aspect of the above method for detecting a spectrum of a urine component, the detecting urine based on the collected spectrum information of the urine includes:

acquiring wavelength values of the spectrum information, and arranging the spectrum information with different wavelengths into a first sequence according to a first preset mode, wherein the first sequence comprises a first noise area and a first characteristic peak area;

acquiring intensity values of the spectrum information, and arranging the spectrum information of the first sequence into a second sequence according to a second preset mode;

performing smooth filtering processing on the spectral information of the second sequence;

sequencing the spectrum information of the second sequence after the smoothing filtering treatment according to a third preset mode and defining the spectrum information as a third sequence, wherein the third sequence comprises a second noise area and a second characteristic peak area;

acquiring a first amount of target spectrum information corresponding to the second characteristic peak region of the third sequence;

and carrying out urine detection according to the first amount of target spectrum information.

As a preferable aspect of the method for detecting a spectrum of a urine component, the step of performing smoothing filtering processing on the second series of spectrum information includes:

continuously acquiring a second preset amount of spectrum information in a second sequence according to the intensity decreasing mode;

calculating the spectrum noise level according to the second preset amount of spectrum information;

calculating a filtering window width according to the spectral noise level;

and carrying out smooth filtering processing according to the width of the filtering window.

As a preferable aspect of the above method for detecting a spectrum of urine components, the calculating a spectrum noise level according to a second preset amount of spectrum information includes:

calculating the average intensity value and the standard deviation intensity value of the second preset amount of spectral information;

calculating a spectral noise level from the mean intensity value and the standard deviation intensity value.

As a preferable mode of the above method for detecting a urine component by spectrum, the detecting urine based on the first amount of target spectrum information includes:

performing ranking detection or fuzzy detection or accurate detection mainly based on the ranking detection and assisted by the fuzzy detection according to the first amount of target spectrum information;

wherein the ranking detection comprises:

a third predetermined amount of spectral information is obtained in a decreasing intensity manner and a fourth predetermined amount of spectral information is obtained in a decreasing intensity manner,

removing the third preset amount of spectral information and the fourth preset amount of spectral information, and then carrying out urine detection;

the blur detection includes:

ordering the first amount of target spectrum information according to a fourth preset mode and defining the first amount of target spectrum information as a third sequence,

determining P intensity sudden increase abnormal ranges with different intensities and Q intensity sudden decrease abnormal ranges with different intensities in a third sequence according to a fifth preset mode, wherein P, Q are positive integers;

acquiring different amounts of spectral information within the P intensity abrupt intensity increase abnormal ranges respectively, wherein the intensity of the spectral information is inversely proportional to the amount of the spectral information acquired according to the intensity, and acquiring different amounts of spectral information within the Q intensity abrupt intensity decrease abnormal ranges respectively, wherein the intensity of the spectral information is proportional to the amount of the spectral information acquired according to the intensity,

and carrying out urine detection after the acquired spectral information is reserved or removed according to preset conditions.

In conclusion, the beneficial effects of the invention are as follows:

according to the spectral detection method for the urine components, urine is injected into a sample detection chamber, light of a background light source is controlled to enter the sample detection chamber through the wall of the sample detection chamber, spectral information of the urine is collected through the sample detection chamber, and urine detection is carried out according to the collected spectral information of the urine. The invention directly injects urine into the sample detection chamber for detection, does not need to manually adjust the placement position of the urine sample, has simple detection flow, can collect the spectral information of the urine, stores the collected spectral information of the urine sample, can transfer the spectral information to a detection position for detection, and has higher detection accuracy. In addition, in order to avoid detection errors, the spectral information can be called to perform secondary verification.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the embodiments of the present invention will be briefly described below, and for those skilled in the art, without any creative effort, other drawings may be obtained according to the drawings, and these drawings are all within the protection scope of the present invention.

FIG. 1 is a perspective view of an intelligent toilet of the present invention;

FIG. 2 is a side view of the intelligent toilet of the present invention;

FIG. 3 is an internal structural view of the intelligent toilet of the present invention;

FIG. 4 is an exploded view of the intelligent toilet of the present invention;

FIG. 5 is a perspective view of a urine sampling head of the present invention;

FIG. 6 is a schematic view of the structure of the elastic member of the present invention;

FIG. 7 is a schematic structural view of the upper portion of the round sampling head body according to the present invention;

FIG. 8 is a schematic structural view of the upper portion of the concave sampling head body according to the present invention;

FIG. 9 is a schematic structural view of the upper portion of the planar sampling head body according to the present invention;

FIG. 10 is a schematic view of the urine sampler of the present invention;

FIG. 11 is a perspective view of the changeover mechanism of the present invention;

FIG. 12 is a first schematic view of a replaceable reagent consumable cartridge according to the present invention;

FIG. 13 is a second schematic structural view of the replaceable reagent consumable cartridge of the present invention;

FIG. 14 is an internal structural view of a replaceable reagent consumable cartridge according to the present invention;

FIG. 15 is a first schematic view of a disposable storage container according to the present invention;

FIG. 16 is a second schematic view of the disposable storage container of the present invention;

FIG. 17 is an internal structural view of the consumable material storing cartridge of the present invention;

FIG. 18 is a perspective view of a microfluidic detection chip according to the present invention;

FIG. 19 is an internal structural view of a microfluidic detection chip according to the present invention;

FIG. 20 is an exploded view of a microfluidic detection chip according to the present invention;

FIG. 21 is a perspective view of a microscopic image information acquisition module of the present invention;

FIG. 22 is a view of the interior of the microscope body according to the present invention;

FIG. 23 is a perspective view of a microscopic image acquisition module of the present invention;

FIG. 24 is a perspective view of an optical information acquisition module of the present invention;

FIG. 25 is a diagram showing the positional relationship between the optical information acquisition assembly and the microfluidic chip according to the present invention;

FIG. 26 is a diagram of the internal structure of a fluorescence/spectroscopy microfluidic detection chip according to the present invention;

FIG. 27 is an exploded view of a fluorescence/spectroscopy microfluidic detection chip according to the present invention;

FIG. 28 is a schematic diagram of an electrochemical detection chip with different locations of a reaction portion and a conductive portion according to the present invention;

FIG. 29 is a schematic view of the structure of a reaction part according to the present invention;

FIG. 30 is a schematic view of a conductive portion according to the present invention;

FIG. 31 is a schematic view of an electrochemical detection chip having a reaction portion and a conductive portion on the same side according to the present invention;

FIG. 32 is a first schematic view of a body fluid electrochemical detection module according to the present invention;

FIG. 33 is a second schematic structural view of a body fluid electrochemical detection module according to the present invention;

FIG. 34 is an exploded view of a body fluid electrochemical detection module according to the present invention;

FIG. 35 is a first internal view of the electrochemical detection module for body fluid according to the present invention;

FIG. 36 is a second schematic view of the electrochemical detection module for body fluid according to the present invention;

FIG. 37 is a schematic view of the structure of a reaction chamber according to the present invention;

FIG. 38 is a perspective view of an electrochemical body fluid testing device in accordance with the present invention;

FIG. 39 is an exploded view of an electrochemical body fluid testing device in accordance with the present invention;

FIG. 40 is a schematic diagram of a system for rapid detection of human biochemical indicators according to embodiment 15 of the present invention;

FIG. 41 is a flow chart showing the steps of a urine test method based on microscopic images in accordance with example 16 of the present invention;

FIG. 42 is a schematic flow chart showing the steps of the urine detection method based on microscopic images after step S120 in example 16;

FIG. 43 is a flowchart illustrating steps S150 of embodiment 16 according to the present invention;

FIG. 44 is a flowchart illustrating steps of a method for detecting a component of urine using a fluorescent reagent according to example 17 of the present invention;

FIG. 45 is a flowchart illustrating steps of a method for detecting a component of urine based on a fluorescent reagent in accordance with example 17, after step S220;

FIG. 46 is a flow chart illustrating steps of a method for spectral detection of urine constituents according to embodiment 18 of the present invention

FIG. 47 is a flow chart illustrating the steps of the electrochemical urine detection method according to example 19 of the present invention;

FIG. 48 is a flowchart illustrating steps S450 of step 19 according to an embodiment of the present invention;

FIG. 49 is a diagram showing the Euler distances between two time series according to embodiment 19 of the present invention;

parts and numbering in the drawings:

100. a toilet body;

110. a base; 120. a toilet bowl;

200. a toilet seat;

210. a third bearing;

300. a toilet cover body;

310. a toilet front cover; 311. a first bearing; 320. a toilet rear cover; 321. a first rotating shaft;

400. a urine sampler;

410. a urine sampling head;

411. a sampling head body; 411a, a through hole; 411b, the upper part of the sampling head; 411c, the lower part of the sampling head;

412. a connecting mechanism; 412a, an elastic member; 412b, a groove; 412c, a protrusion; 412d, a stop mechanism;

420. a transfer mechanism; 421. a changeover mechanism body; 421a, a transfer groove; 421b, a mounting cavity;

500. sampling a microflow pump;

600. a consumable storage box;

610. a consumable storage box body; 611. an electronic tag reader; 612. a transparent window; 613. a thimble; 614. a consumable storage box liquid outlet;

620. the reagent consumable box can be replaced;

621. a consumable cartridge body; 621a, an electronic tag; 621b, a transparent member; 621c, mounting holes;

622. a consumable cartridge reagent inlet; 623. a consumable cartridge reagent outlet;

624. a consumable cartridge seal; 624a, a reset piece; 624b, flick needle; 624c, a cover plate;

625. a first gap; 626. a second gap;

630. an upper cover of the consumable storage box;

700. a urine detection module;

710. a microfluidic detection chip;

711. detecting a chip body; 711a, a first device chamber; 711b, a light emitting device; 711c, a second device chamber; 711d, temperature adjusting devices; 711e, device seal; 711f, first chamber cover; 711g, a second chamber cover; 711h, an excitation light filter layer;

712. detecting a sample inlet of the chip; 713. a sample detection chamber; 714. a first microchannel; 715. detecting a chip sample outlet; 716. a second microchannel;

720. a microscopic image acquisition module;

730. a microscopic image information acquisition module;

731. a microscope body;

732. a lens group;

733. a zoom assembly; 733a, a first magnifying lens; 733b, a second magnifying lens; 733c, protective glasses;

734. a filter assembly; 734a, a first filter; 734b, a first filter;

735. an object stage;

736. a microscopic optical information acquisition component;

740. an optical information acquisition module;

741. an optical information acquisition component;

750. an electrochemical body fluid detection device;

760. an electrochemical detection chip;

761. an insulating substrate; 761a, a first spacer; 761b, a second spacer;

762. a chip electrode; 762a, a reaction part; 762b, a conductive part; 762c, a reaction part liquid outlet;

770. a body fluid electrochemical detection module;

771. detecting the module body; 771a, reaction zone; 771b, connecting region; 771c, reaction chamber; 771d, cavity; 771e, sealing structure; 771f, collecting tank; 771g, sample injection pipeline mounting holes;

772. an electrochemical sample inlet;

773. connecting the electrodes;

774. an electrochemical sample outlet;

775. a sample introduction pipeline; 775a, a sample injection pipeline body; 775b, sample inlet pipe outlet; 775c, bending part;

776. a sample outlet pipeline;

780. a urine transport conduit.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. In the description of the present invention, it is to be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element. In case of conflict, the embodiments of the present invention and the various features of the embodiments may be combined with each other within the scope of the present invention.

Example 1

Referring to fig. 1 and 2, an embodiment of the present invention discloses an intelligent toilet, which can be used as a general toilet, and can also be used in the field of urine detection to detect urine of a user, and can be specifically applied to places such as a home, an enterprise, or a hospital, for example, in the home: the patient needs long-term recuperation, and need often detect urine information, through analysis urine data in order to confirm patient's self health status, although the urinalysis equipment of hospital is complete, doctor's specialty is also higher, however, the expense that will spend at the hospital is also more, at present, there are more families to compare the data, this expense of hospital can bring a huge burden for whole family, if adopt the intelligent closestool of this application, the patient treats and can carry out urine detection at home, not only saved the various expenses of hospital, and, also, more make things convenient for the family to take care of the patient, at home, the family both can compromise personal affairs and family affairs, also have more time to take care of the patient. In addition, patients with certain self-care ability can also be independently maintained at home, and can know the physical health condition of the patients in real time according to the needs.

If in the hospital, the detection of need lining up earlier, in the flow of detection, can only be by oneself by the user at the bathroom like the lavatory in-process take a sample, after the sample is accomplished, give the sample medical staff and carry out the analysis of detection, the sample flow of whole process is inconvenient to it needs the waste time to line up, if adopt the intelligent closestool that this application provided, the customer need not be at the hospital and is rushing to ripples, need not line up, and the flow of sample is also fairly simple, and it is more convenient to use, labour saving and time saving.

The intelligent toilet comprises a toilet body 100, a toilet seat 200, a toilet lid 300, a urine sampler 400 and a urine detection module. The toilet body 100 serves as a base body of the intelligent toilet, has a function of bearing various devices, and can also realize the defecation function of a common toilet. Toilet seat 200 sets up on closestool body 100, toilet seat 200 is high with human laminating degree, toilet seat 200 can increase the comfort level of user when like the lavatory, if when weather is cold, sheathe toilet seat 200 on the closestool, can avoid ice-cold closestool direct and human contact, furthermore, the cover has toilet seat 200's closestool more sanitary health, certain water pressure has during the closestool bath, can lead to the bacterium to spatter in disorder, toilet seat 200 then has certain isolation and protecting effect. The toilet cover 300 can ensure the sanitation of the toilet, and when the toilet is not in use, the toilet cover 300 is closed to seal the toilet body 100, so that impurities such as bacteria, dust or liquid are prevented from entering the toilet. The urine sampler 400 may be disposed in the toilet body 100, or may be disposed above the toilet body 100 on a path of urine flowing into the toilet body 100, for sampling the urine. The urine detection module is disposed on the toilet lid 300 or the toilet body 100, and is used for sampling urine sampled by the urine sampler 400.

To facilitate understanding of the structure of the intelligent toilet, the components of the intelligent toilet will now be further described as follows:

the toilet body 100 includes a base 110 and a bowl 120. The lower bottom surface of the base 110 is contacted with the ground, the upper surface of the base 110 is contacted with the toilet seat ring 200, the length and the width of the upper surface of the base 110 are both larger than those of the lower bottom surface, and the large toilet space can be realized while the occupied space is small. The urinal groove 120 is a conical structure, the conical structure is arranged in an inverted manner, the conical vertex is positioned below the conical bottom surface, the closestool body 100 further comprises a sewer pipeline, one end of a pipeline opening of the sewer pipeline is communicated with the conical vertex of the urinal groove 120, the other end of the pipeline opening of the sewer pipeline is connected into the urinal, and the conical structure can effectively collect excrement and urine in the urinal groove 120 and cleaning liquid during flushing.

The toilet cover 300 includes a front cover 310 and a rear cover 320, the rear cover 320 is fixed to the toilet body 100 and located at the rear of the toilet body 100, the front cover 310 is rotatably connected to the toilet body 100 or the rear cover 320 and located at the front of the toilet, covers the toilet seat 200, and covers the toilet bowl 120. Preferably, in this embodiment, the toilet front cover 310 is rotatably connected to the toilet rear cover 320, specifically, first rotating shafts 321 are disposed on two sides of the toilet rear cover 320, a first bearing 311 is disposed on the toilet front cover 310, the first rotating shafts 321 are mounted on the first bearing 311, and the toilet front cover 310 can rotate back and forth relative to the toilet rear cover 320, so that the toilet front cover 310 covers the toilet bowl 120 to protect the toilet, and the toilet front cover 310 is lifted, so that a user can go to the toilet or perform urine detection. When the toilet front cover 310 is rotatably connected to the toilet body 100, a second rotating shaft (not shown) is fixedly disposed on the toilet body 100, a second bearing (not shown) is disposed on the toilet front cover 310, the second bearing is sleeved on the second rotating shaft, and the toilet front cover 310 can rotate back and forth relative to the toilet body 100.

When the toilet seat 200 covers the toilet bowl 120, the toilet seat 200 is disposed between the toilet seat 310 and the toilet body 100, the toilet cover 300 can also protect the toilet seat 200, the toilet seat 200 is rotatably connected to the toilet body 100 or the toilet cover 300, and the toilet seat 200 is provided with the third bearing 210, so that when the toilet seat 200 is connected to the toilet cover 300, the third bearing 210 is connected to the first rotating shaft 321, and when the toilet seat 200 is connected to the toilet body 100, the third bearing 210 is connected to the second rotating shaft.

Intelligent closestool still includes consumptive material receiver 600, and consumptive material receiver 600 is used for providing the detection condition for the urine detection module. The consumable housing cartridge 600 includes a plurality of replaceable reagent consumable cartridges 620, with reagents for mixing with urine contained within the plurality of replaceable reagent consumable cartridges 620.

The urine detection module includes an optical urine detection module, and the optical urine detection module is disposed on the toilet body 100 or the toilet cover 300. The optical urine detection module comprises a microscopic image acquisition module 720, a fluorescent image acquisition module and a spectral information acquisition module, wherein the microscopic image acquisition module 720 performs urine detection by adopting a urine detection method based on a microscopic image, the fluorescent image acquisition module performs urine detection by adopting a urine component detection method based on a fluorescent reagent, and the spectral information acquisition module performs urine detection by adopting a spectral detection method of urine components. The urine detection module further includes a chemical urine detection module, and the chemical urine detection module is disposed on the toilet body 100 or the toilet cover 300. The chemical urine detection module comprises a dry chemical urine detection module and an electrochemical body fluid detection device 750, and the electrochemical body fluid detection device 750 of the embodiment performs urine detection by using a urine electrochemical detection method described later.

The intelligent closestool further comprises a control system, a urine transmission pipeline 780 and a sampling micro-flow pump 500, wherein the urine transmission pipeline 780 is used for transmitting urine, the sampling micro-flow pump 500 can remove bubbles in the urine and can also quantitatively acquire and convey the urine, the control system is used for controlling the urine sampler 400 to sample the urine, and the urine detection module 700 is controlled to detect the urine.

Example 2

The intelligent closestool comprises a urine sampler 400, wherein the urine sampler 400 can be arranged in the closestool body 100 and also can be arranged above the closestool body 100 and is used for sampling urine flowing into the closestool.

Referring to fig. 10 and 11, the urine sampler 400 of the present invention includes an adapter 420 and a urine sampling head 410, wherein the urine sampling head 410 is detachably connected to the adapter 420, and the urine sampling head 410 directly contacts with urine to sample urine.

Specifically, urine sampling head 410 includes elastic component 412a, and adapter 420 includes adapter body 421, is provided with installation cavity 421b on adapter body 421, and elastic component 412a inserts installation cavity 421b, and elastic component 412a and installation cavity 421b elastic connection can insert or take out urine sampling head 410 under the exogenic action. When urine sampler 400 blocks up or needs to be cleaned, urine sampling head 410 can be very convenient dismantlement to wash, repair or change, easy operation is convenient to urine sampling head 410 and urine sampler 400.

Example 3

Referring to fig. 5 and fig. 6, an embodiment of the invention discloses a urine sampling head 410, the urine sampling head 410 includes a sampling head body 411 and a connection mechanism 412, the sampling head body 411 is provided with a through hole 411a for preventing foreign matter from entering, when external urine enters into the sampling head body 411 through the through hole 411a, the through hole 411a can filter the urine to block the foreign matter in the urine outside the sampling head body 411, the connection mechanism 412 is arranged at one end of the sampling head body 411, the connection mechanism 412 is provided with a detachable structure, the detachable structure enables the urine sampling head 410 to be detachably connected with the whole sampling device, the whole sampling device is a device for urine sampling, the urine sampling head 410 and the whole sampling device are connected under the action of external force, the whole sampling device is driven, the urine sampling head 410 performs urine sampling, the urine sampling head 410 and the whole sampling device are detached, can wash, repair or change urine sampling head 410, the sampling device is whole, simple structure, the simple operation.

To facilitate understanding of the structure of the urine sampling head 410, the components of the urine sampling head 410 will now be described separately as follows:

the concrete structure of detachable construction does not do the restriction here, as long as enable urine sampling head 410 and the whole detachable connection of sampling device can, in this embodiment, detachable construction includes at least one of detachable construction such as elastic component, helicitic texture and buckle, and is preferred, and the elastic component is chooseed for use to detachable construction, and the elastic component connection performance is good and be convenient for installation and dismantlement.

Further, referring to fig. 5 to 7, the connection mechanism 412 is provided with an elastic member 412a, when the urine sampling head 410 is connected to the urine sampler 400, the elastic member 412a is engaged with the urine sampler 400, the elastic force of the elastic member 412a acts on the urine sampler 400, the urine sampler 400 also applies an opposite acting force to the elastic member 412a, meanwhile, the elastic member 412a and the urine sampler 400 have a friction force, the urine sampling head 410 is stably connected to the urine sampler 400 under the action of the friction force, when the urine sampling head 410 needs to be detached, an external force is applied to the urine sampling head 410 and the urine sampler 400, the applied external force is greater than the friction force between the elastic member 412a and the urine sampler 400, and the urine sampling head 410 can be pulled out.

The elastic member 412a is an independent component, the connecting mechanism 412 is provided with a groove 412b or a protrusion 412c, the elastic member 412a is matched with the groove 412b or the protrusion 412c, the elastic member 412a is sleeved on the groove 412b or the protrusion 412c and is fixed by the groove 412b or the protrusion 412c, when the elastic member 412a needs to be installed, the elastic member 412a is opened under the action of external force, so that the elastic member 412a can penetrate through the connecting mechanism 412 to enter the periphery of the groove 412b or the protrusion 412c, and at the moment, the external force is removed, so that the elastic member 412a is sleeved on the groove 412b or the protrusion 412 c. One or more elastic members 412a may be provided, when the number of the elastic members 412a is one, the elastic members 412a are matched with the connecting mechanism 412, and the elastic members 412a may slide out of the grooves 412b or the protrusions 412c under the action of an external force, so that the installation cannot be completed. Further, the elastic member 412a includes: when the elastic pieces 412a in different shapes are selected, it is necessary to ensure that each elastic piece 412a can be fully connected with the groove 412b or the protrusion 412c, preferably, a plurality of elastic pieces 412a in the same shape are selected, such as: the O-ring, contact with urine sampler 400 will be more complete and better processed. It is understood that the shape of the groove 412b or the protrusion 412c is adapted to the shape of the elastic member 412a, and the groove 412b is selected to match the protrusion 412c when the elastic member 412a is in the structure of the protrusion 412c, and the protrusion 412c is selected to match the groove 412b when the elastic member 412a is in the structure of the groove 412 b.

In one embodiment, the elastic member 412a is integrated with the connecting mechanism 412, and the elastic member 412a is integrated with the connecting mechanism 412. in this case, the connecting mechanism 412 does not include the groove 412b or the protrusion 412c, the elastic member 412a may be a ring-shaped structure covering the circumference of the connecting mechanism 412, and the contact area between the elastic member 412a and the urine sampler 400 is larger, so that the connection is more stable.

Elastic component 412a has the effect of fixed urine sampling head 410 in addition, still has sealing effect, elastic component 412a inner circle and recess 412b or protruding 412c closely laminate, elastic component 412a outer lane and urine sampler 400 closely laminate, elastic component 412a itself has water-proof effects, so, elastic component 412a can prevent that the urine after filtering from flowing out from coupling mechanism 412 to assemble the urine in sampling head body 411, provide sufficient urine sample for the urine detection module.

In this embodiment, the sampling head body 411 is made of metal, ceramic or plastic, and the like, but not limited thereto, preferably, the sampling head body 411 is made of a material with high corrosion resistance, such as: a ceramic.

Referring to fig. 5, the sampling head body 411 includes a sampling head upper portion 411b and a sampling head lower portion 411c, the through hole 411a is disposed on the sampling head upper portion 411b, the sampling head upper portion 411b directly contacts with urine, the urine flows into the sampling head body 411 through the through hole 411a, the shape of the through hole 411a is not limited herein, and preferably, the through hole 411a may be a geometric figure such as a circle, a square, an ellipse, and a triangle, the through hole 411a is disposed to prevent foreign matter from entering the sampling head body 411, the foreign matter may be coarse-grained matter, and the through hole 411a may filter any matter whose minimum width is greater than the maximum width of the through hole 411 a. Further, through-hole 411a sets up in sample head upper portion 411b according to certain mode interval of arranging, can be the array and arrange, also can misplace and arrange, and the range is even as far as possible, make full use of sample head upper portion 411b area for sample head upper portion 411b can set up more through-holes 411a, and the filter effect is also good when the feed liquor volume is many.

Referring to fig. 7, the sampling head body 411 further comprises a urine transfer pipe 780, and the urine transfer pipe 780 is used for transferring urine passing through the upper sampling head part 411 b. After urine enters the urine transport pipe 780, the urine transport pipe 780 transports the urine to the urine detection module for urine detection.

Further, referring to fig. 5 to 9, the upper portion 411b of the sampling head is planar, concave or convex. The urine is directly contacted with the upper part 411b of the sampling head, and when the upper part 411b of the sampling head is of a planar structure, the sampling head is convenient to process, so that the cost can be saved; when the upper portion 411b of the sampling head is of a concave structure, urine left on the upper portion 411b of the sampling head can flow into the sampling head body 411 along the concave structure as much as possible, so that the loss of the urine is less, the urine collecting speed is high, and the detection efficiency is high; when the upper portion 411b of the sampling head is a convex structure, the internal volume of the sampling head body 411 is larger, the liquid storage capacity of the urine sampling head 410 is larger, and a sufficient urine sample can be provided. The three structures are selected according to actual conditions, and because the urine sampling head 410 is independently arranged, the sampling heads at the three stages can be processed, and more suitable sampling heads can be selected under different conditions.

Further, the sampling head lower portion 411c completely or partially receives urine filtered through the through-hole 411 a.

In the situation that the part 411c of the lower part of the sampling head contains the filtered urine, the part 411c of the lower part of the sampling head is sealed or unsealed, the urine passes through the filtering structure after being filtered by the through hole 411a and directly flows downwards without converging in the filtering structure, in this case, the setting position of the urine transmission pipeline 780 is not fixed, the opening of the pipeline is arranged on the path in which the urine flows, part of the urine directly flows into the urine transmission pipeline 780, at the moment, the urine sample obtained by the urine sampler 400 is all fresh urine, the urine detection module detects according to the fresh urine, and the reference value of the detection result is higher.

In the case that the liquid collection chamber completely contains the filtered urine, the lower portion 411c of the sampling head is fully sealed and the urine is collected in the sampling head body 411. The end of urine transfer pipe 780 is laminated or is close to the bottom of sampling head lower part 411c, and urine transfer pipe 780 can be more stable absorb urine, and the urine bubble of absorption is less, and the urine volume of absorption is bigger, can improve detection efficiency.

Further, the connection mechanism 412 includes a limiting mechanism 412d, and the limiting mechanism 412d is used for limiting the assembling angle of the urine sampling head 410. Specifically, this stop gear 412d is spacing boss or spacing recess, and changeover mechanism 420 and coupling mechanism 412 of urine sampler 400 are connected, and changeover mechanism 420 is last to be provided with spacing boss or spacing recess assorted switching recess 421a or switching boss, and the boss cooperates with the recess, has restricted the assembly angle of urine sampling head 410 to, can prevent that the urine sampling head 410 of installing from taking place to rotate, connect more stably.

The sampling head body 411 and the connecting mechanism 412 are integrally arranged or detachably connected. The integrated arrangement production steps are fewer, the processing difficulty of the detachably connected sampling head body 411 and the connecting mechanism 412 is lower, and in the embodiment, the sampling head body 411 and the connecting mechanism 412 are integrally arranged.

Example 4

The intelligent closestool further comprises a sampling micro-flow pump 500, the sampling micro-flow pump 500 is arranged on a urine transmission pipeline between the urine sampler 400 and the urine detection module, gas in urine can be removed, real-time and controllable setting can be realized for urine detection through the setting of the sampling micro-flow pump 500, and convenient and accurate urine detection and control are realized.

Example 5

Referring to fig. 12 to 14, an embodiment of the present invention discloses a replaceable reagent consumable cassette 620, where the replaceable reagent consumable cassette 620 includes a consumable cassette body 621, a consumable cassette reagent inlet 622, a consumable cassette reagent outlet 623, and a consumable cassette sealing element 624, where the consumable cassette reagent inlet 622 and the consumable cassette reagent outlet 623 are both disposed on the consumable cassette body 621, the consumable cassette sealing element 624 is disposed on the consumable cassette reagent outlet 623, when the consumable cassette body 621 is installed, the consumable cassette sealing element 624 is opened by an external force, the consumable cassette reagent outlet 623 is communicated with the outside, and reagents in the replaceable reagent consumable cassette 620 can flow out from the consumable cassette reagent outlet 623 and be mixed with urine in a next process; when the consumable cartridge body 621 is disassembled, the consumable cartridge sealing member 624 is reset, the consumable cartridge reagent outlet 623 is sealed, and the reagent cannot flow out of the consumable cartridge reagent outlet 623 and is stored in the replaceable reagent consumable cartridge 620. The setting of consumptive material box sealing member 624 for removable reagent consumptive material box 620 can be dismantled at will and dismantle conveniently, and the removable reagent consumptive material box 620 leakproofness after the dismantlement is good, is convenient for add or change reagent.

To facilitate understanding of the structure of the replaceable reagent consumable cassette 620, the consumable cassette body 621, the consumable cassette reagent inlet 622, the consumable cassette reagent outlet 623, and the consumable cassette seal 624 are described separately as follows:

the shape of consumptive material box body 621 can be the tetrahedron, the cone, cylinder or other polyhedral structure, its structure does not do the injecing, in this embodiment, consumptive material box body 621 is preferably the cuboid structure, when removable reagent consumptive material box 620 quantity is a plurality of, the installation can be compacter between the consumptive material box body 621 of cuboid structure, it is various because of intelligent closestool inner structure, the space is limited, consumptive material box body 621 compact installation can rationally be used for intelligent closestool's inner space, and, rectangular consumptive material box body 621's volume is bigger, under the condition that occupies the same space, rectangular's consumptive material box body 621 capacity is bigger, the reagent that can hold is more.

Consumable box reagent entry 622 sets up on consumable box body 621, can locate consumable box body 621 top surface, also can locate consumable box body 621 side, when consumable box reagent entry 622 locates consumable box body 621 side, should set up as far as possible in the eminence, in this embodiment, it is preferred, consumable box reagent entry 622 locates consumable box body 621 top surface, the position that consumable box reagent entry 622 set up is higher, be difficult to influence the storage of reagent more, if: the consumable box reagent inlet 622 is arranged on the side surface of the consumable box body 621, the liquid level of the reagent is lower than the lowest point of the consumable box reagent inlet 622, when the consumable box reagent inlet 622 is arranged on the top surface of the consumable box body 621, the reagent can be filled in the whole container, and the situation that the liquid level height is limited does not exist.

Referring to fig. 13, an electronic tag 621a for reading reagent information is disposed outside the consumable cartridge body 621, and when a reagent is contained in the replaceable reagent consumable cartridge 620, the electronic tag 621a can detect the reagent and read information related to the reagent, where the information related to the reagent includes information such as a reagent type, a reagent capacity, a reagent quality, and a reagent storage time. Further, the electronic tag 621a is an RFID or NFC tag, and an RFID technology-a line Radio Frequency Identification (Radio Frequency Identification, RFID) is one of automatic Identification technologies, and performs non-contact bidirectional data communication in a Radio Frequency manner, and reads and writes a recording medium (an electronic tag or a Radio Frequency card) in the Radio Frequency manner, thereby achieving the purposes of identifying an object and exchanging data. NFC technology, Near Field Communication technology (Near Field Communication), is an emerging technology, and devices (e.g., mobile phones) using NFC technology can exchange data when they are close to each other, and are integrated and evolved from non-contact Radio Frequency Identification (RFID) and interconnection and interworking technology, and by integrating functions of an inductive card reader, an inductive card and point-to-point Communication on a single chip, applications such as mobile payment, electronic ticketing, door access, mobile identity recognition, anti-counterfeiting, and the like are implemented using a mobile terminal. Preferably, the RFID electronic tag is selected for use in the embodiment, so that the cost is low and the stability is high.

Referring to fig. 12, a transparent member 621b for checking the reagent volume is disposed outside the consumable box body 621, and the transparent member 621b may be glass or transparent adhesive tape, which has both sealing and observation functions. Further, the transparent member 621b is provided with a scale, and the range of the scale covers the height range of the reagent, so as to accurately detect the reagent volume.

Referring to fig. 14, a mounting hole 621c is further formed in the consumable cartridge body 621, the consumable cartridge sealing member 624 includes a resetting member 624a, an eject pin 624b and a cover plate 624c, the resetting member 624a is disposed in the mounting hole 621c, the resetting member 624a reciprocates in the mounting hole 621c, the eject pin 624b is disposed at a lower end of the resetting member 624a, the resetting member 624a drives the eject pin 624b to reciprocate, and the eject pin 624b can close and open the reagent outlet 623 of the consumable cartridge during the reciprocating motion. A cover plate 624c is disposed on an upper end of the reset member 624a, the cover plate 624c is used to support the reset member 624a and limit a reset position of the reset member 624a, the reset member 624a is fixedly disposed on the cover plate 624c, the cover plate 624c is fixedly disposed in the replaceable reagent consumable cartridge 620, and the direction of reciprocating the reset member 624a is far away from the cover plate 624 c.

When the consumable box body 621 is installed, the elastic needle 624b compresses the reset piece 624a under the action of external force, the elastic needle 624b does not contact with the consumable box reagent outlet 623, the cover plate 624c is communicated with the reset piece 624a, and the reagent flows out of the consumable box reagent outlet 623 through the cover plate 624c, the reset piece 624a and the elastic needle 624b from the interior of the consumable box body 621. Further, a plurality of small holes for flowing the reagent are formed in the cover plate 624c, and the plurality of small holes are arranged on the cover plate 624c, and have a frame shape, so that the reset member 624a can be supported, and the reagent can also flow. A first gap 625 is formed between the resetting piece 624a and the wall of the mounting hole 621c, and reagent can flow out of the reagent outlet 623 of the consumable box through the first gap 625. In this embodiment, the reset element 624a is preferably a spring, the width of the spring is smaller than the diameter of the mounting hole 621c, the middle part of the spring is hollow, and the reagent can flow into the spring through the cover body, flow out of the spring to the first gap 625, and flow out through the first gap 625. A second gap 626 is formed between the elastic needle 624b and the wall of the mounting hole 621c, the first gap 625 is communicated with the second gap 626, the second gap 626 is communicated with the reagent outlet 623 of the consumable box, and the reagent flows into the second gap 626 through the first gap 625 and then flows out of the reagent outlet 623 of the consumable box. In this embodiment, the width of the latch 624b is smaller than the diameter of the mounting hole 621c, the latch 624b is fixedly connected with the reset piece 624a, the latch 624b is sealed with the reset piece 624a, and the reagent can smoothly flow out of the reagent outlet 623 of the consumable cartridge.

The position of the elastic needle 624b corresponds to the position of the reagent outlet 623 of the consumable box, when the consumable box body 621 is disassembled, the external force is removed, the reset part 624a resets, the reset part 624a drives the elastic needle 624b to move downwards until the elastic needle 624b contacts the reagent outlet 623 of the consumable box, the lower end surface of the elastic needle 624b covers the reagent outlet 623 of the consumable box, and the reagent outlet 623 of the consumable box is sealed.

Example 6

Referring to fig. 15 to 17, an embodiment of the invention discloses a consumable storage box 600, the consumable storage box 600 includes a consumable storage box body 610 and an electronic tag card reader 611, a plurality of replaceable reagent consumable cartridges 620 are accommodated in the consumable storage box body 610, a plurality of reagents are accommodated in the replaceable reagent consumable cartridges 620, the plurality of reagents can be simultaneously fused with sample urine to form a plurality of different mixed liquids, so as to perform different types of detection, and improve detection accuracy and range, the electronic tag card reader 611 is disposed on the consumable storage box body 610 and is used for reading electronic tag 621a data on the replaceable reagent consumable cartridges 620, and the electronic tag 621a data includes data of each reagent, so that an operator can timely know relevant information of the reagents.

To facilitate understanding of the structure of the consumable cartridge 600, the consumable cartridge body 610, the electronic tag reader 611, and the replaceable reagent consumable cartridge 620 will now be described separately as follows:

the reagent kind that holds in the consumptive material receiver body 610 is injectd by the operator, and multiple reagent all sets up in removable reagent consumptive material box 620, and convenient operation can carry out unified management, also can the narrow and small inner space of make full use of intelligent closestool. In this embodiment, preferably, the reagent includes four kinds, and after four kinds of reagents respectively mixed with urine, respective mixed solution respectively gets into urine detection module and carries out microscopy, fluorescence detection, spectral detection and electrochemical detection. The range and the precision of urine detection can be improved by various detection modes.

Preferably, referring to fig. 15, the consumable magazine 600 further includes a consumable magazine upper cover 630, and the consumable magazine upper cover 630 is used to seal the consumable magazine body 610. When the consumptive material box needs to be changed, uncover consumptive material receiver upper cover 630, place the consumptive material box in consumptive material receiver body 610, place the back that finishes, adorn consumptive material receiver upper cover 630, realize the sealed to consumptive material receiver body 610. Further, the consumable cartridge is fastened between the consumable cartridge body 610 and the consumable cartridge upper cover 630, realizing fixation of the consumable cartridge.

Referring to fig. 15, a transparent window 612 is disposed on the consumable storage box body 610, the transparent window 612 is used for observing reagent capacity, a transparent part 621b is disposed on the replaceable reagent consumable box 620, the position of the transparent window 612 corresponds to the position of the transparent part 621b on the replaceable reagent consumable box 620, the transparent part 621b corresponds to a reagent in the replaceable reagent consumable box 620, the reagent capacity in the replaceable reagent consumable box 620 can be observed through the transparent part 621b, and in order to observe the reagent capacity in the plurality of replaceable reagent consumable boxes 620 more intuitively, the transparent window 612 needs to be set to observe information reflected by the transparent part 621 b. Furthermore, the transparent window 612 is partially transparent or completely transparent, and the transparency degree of the transparent window 612 determines the observation precision of the operator, and in this embodiment, the transparent window 612 is selected to be completely transparent, so that the observation effect is better. Further, the transparent window 612 may be a transparent member such as glass. Certainly, in order to ensure that the reagent capacity reflected by the transparent piece 621b is more intuitive, the transparent window 612 may be only one window, and no other element is installed in the window, so that the operator can directly observe the reagent capacity reflected by the transparent piece 621b through the window, and the observation effect is better.

Referring to fig. 17, the bottom of the consumable housing cassette body 610 is provided with a plurality of pins 613, and the pins 613 are used for opening the consumable cassette reagent outlet 623 of the replaceable reagent consumable cassette 620. The plurality of pins 613 are located at positions corresponding to the positions of the plurality of pins 624b of the replaceable reagent consumable cartridge 620. The thimble 613 may provide external force for the replaceable reagent consumable cartridge 620, when the replaceable reagent consumable cartridge 620 is installed, the thimble 613 contacts the pogo pin 624b, the reset piece 624a is compressed, the reagent outlet 623 of the replaceable reagent consumable cartridge communicates with the outside, and the reagent in the replaceable reagent consumable cartridge 620 may flow out from the reagent outlet 623 of the replaceable reagent consumable cartridge. When the replaceable reagent consumable cassette 620 is disassembled, the latch 624b moves away from the latch 613 until the latch 613 is not in contact with the latch 624b, no external force is applied to the latch 624b, the reset piece 624a resets, and the latch 624b closes the reagent outlet 623 of the consumable cassette.

Further, please see fig. 16, the consumable housing box body 610 further includes a consumable housing box liquid outlet 614, the position of the consumable housing box liquid outlet 614 corresponds to the position of the consumable box reagent outlet 623, the reagent flows out of the consumable housing box 600 from the consumable box reagent outlet 623 through the consumable housing box liquid outlet 614, the reagent enters the next detection process and is mixed with urine to form a mixed liquid, and the human body condition is determined by the related data of the mixed liquid.

Further, referring to fig. 16, an electronic tag 621a for reading reagent information is disposed outside the consumable cartridge body 621, an electronic tag card reader 611 is disposed on the consumable cartridge body 610, and the position of the electronic tag card reader 611 corresponds to the position of the electronic tag 621a on the replaceable reagent consumable cartridge 620. When the replaceable reagent consumable box 620 is filled with reagents, the electronic tag 621a can detect the reagents and read the relevant information of the reagents, the relevant information of the reagents includes the reagent type, the reagent capacity, the reagent quality, the reagent storage time and other information, and the electronic tag card reader 611 can read and write the reagent information acquired by the electronic tag 621a, thereby realizing the monitoring and correction of the reagent information. Further, the electronic tag 621a is an RFID electronic tag or an NFC electronic tag 621a, the electronic tag card reader 611 is an RFID or NFC electronic tag card reader 611, and the RFID technology-line Radio Frequency Identification (Radio Frequency Identification, RFID) is one of automatic Identification technologies, and performs non-contact bidirectional data communication in a Radio Frequency manner, and performs reading and writing on a recording medium (an electronic tag or a Radio Frequency card) in a Radio Frequency manner, thereby achieving the purpose of identifying an object and exchanging data. NFC technology, Near Field Communication technology (Near Field Communication), is an emerging technology, and devices (e.g., mobile phones) using NFC technology can exchange data when they are close to each other, and are integrated and evolved from non-contact Radio Frequency Identification (RFID) and interconnection and interworking technology, and by integrating functions of an inductive card reader, an inductive card and point-to-point Communication on a single chip, applications such as mobile payment, electronic ticketing, door access, mobile identity recognition, anti-counterfeiting, and the like are implemented using a mobile terminal. Preferably, in this embodiment, the RFID tag and the RFID tag reader are selected, so that the stability is higher.

Example 7

Referring to fig. 18 to 20, an embodiment of the invention discloses a microfluidic detection chip 710 disposed in an optical urine detection module to provide a detection environment for optical urine detection, and specifically, the optical urine detection module includes a microscopic image acquisition module 720, a fluorescent image acquisition module 740, and a spectral image acquisition module, in which the microfluidic detection chip 710 is disposed in all of the three modules.

Referring to fig. 18 and 19, the microfluidic detection chip 710 according to the present invention includes a detection chip body 711, a detection chip inlet 712, a sample detection chamber 713, and a first micro channel 714, where the sample detection chamber 713 is disposed in the detection chip body 711 and is used for accommodating and assisting a detection sample, the first micro channel 714 is disposed in the detection chip body 711, and the sample flows into the sample detection chamber 713 through the first micro channel 714 from the detection chip inlet 712, in this embodiment, the detection chip inlet 712, the sample detection chamber 713, and the first micro channel 714 are sequentially communicated, so that the sample can directly flow into the sample detection chamber 713 through the first micro channel 714 for detection, and the microfluidic detection chip 710 has a high integration level, and does not need to manually transfer the detection sample, and the detection process is simple.

The microfluidic detection chip 710 is detachably connected with the microscopic image acquisition module 720, the fluorescent image acquisition module 740 and the spectral image acquisition module, and when the detection chip body 711 in the microfluidic detection chip 710, the fluorescent image acquisition module 740 and the spectral image acquisition module are polluted, the microfluidic detection chip 710 can be detached, and a polluted detection device can be replaced, so that the accuracy of a detection result is ensured.

The microfluidic chip 710 is easy to clean, and a cleaning solution is injected into the first micro-channel 714, and the cleaned sample flows out of the second micro-channel 716, so that the detection chip body 711, the first micro-channel 714 and the second micro-channel 716 can be cleaned more conveniently.

To facilitate understanding of the structure of the microfluidic detection chip 710, the detection chip body 711, the detection chip sample inlet 712, the sample detection chamber 713, and the first microchannel 714 are described as follows:

referring to fig. 18-20, the sample detection chamber 713 is partially or fully transparent. The sample detection chamber is used for assisting in detecting a sample, the sample detection chamber 713 comprises an upper chamber wall, a lower chamber wall and a side wall, when the side wall is transparent, and the upper chamber wall or the lower chamber wall is also transparent, the sample is introduced into the detection chamber, an external light source can enter the sample detection chamber 713 through the side wall, the transparent upper chamber wall or the lower chamber wall, and light is reflected out of the sample detection chamber 713 through the transparent upper chamber wall or the transparent lower chamber wall, so that a light source environment can be provided for sample detection. When the upper cavity wall and the lower cavity wall are transparent, light provided by the light source can penetrate through the upper cavity wall and the lower cavity wall from one side of the upper cavity wall or the lower cavity wall, and a light environment is provided for sample detection. The microfluidic detection chip 710 in this embodiment is used for assisting the sample to perform detection, has high integration level and simple structure, and reduces the detection cost.

In this embodiment, the first micro channel 714 is disposed in the detection chip body 711, the sample detection chamber 713 is located at one section of the first micro channel 714, the detection chip injection port 712 is disposed at one end of the first micro channel 714 and is communicated with the outside, the sample flows into the first micro channel 714 from the detection chip injection port 712 and directly enters the sample detection chamber 713, the detection module can directly detect urine in the sample detection chamber 713, the detection sample does not need to be manually transferred, and the detection process is relatively simple.

The detection chip further comprises: a detection chip sample outlet 715 and a second micro-channel 716, wherein the detection chip sample outlet 715 is arranged at one end of the second micro-channel 716 and is communicated with the outside, and the sample flows out of the detection chip sample outlet 715 from the sample detection chamber 713 through the second micro-channel 716. The second micro-channel 716 is disposed in the detection chip body 711, the first micro-channel 714 is communicated with the second micro-channel 716, the sample enters the sample detection chamber 713 from the first micro-channel 714, then flows into the second micro-channel 716 from the sample detection chamber 713, and finally flows out of the detection chip through the detection chip outlet 715, thereby completing the sample detection. In this embodiment, the detection chip includes both the first micro-channel 714 and the second micro-channel 716, if the sample is the detection sample, the sample is detected by the first micro-channel 714 to form a waste liquid flowing out from the second micro-channel 716, the detection chip can be reused, and if the sample is the cleaning liquid, the interior of the detection chip can be cleaned for the next use.

In another embodiment, the detection chip comprises only the first microchannel 714 and no second microchannel 716, the sample can not enter and the detection chip is a disposable product.

Referring to fig. 19 and 20, the detection chip body 711 further includes a device cavity for accommodating a detection device, and the detection device is used for providing a detection environment for sample detection.

Further, the device chamber includes a first device chamber 711a, the first device chamber 711a is configured to accommodate a light emitting device 711b, the light emitting device 711b emits a light source for detecting the sample, the light source is projected onto the sample, and the sample projected by the light source is transmitted out of the microfluidic detection chip 710, so that the optical urine detection module detects and analyzes the sample. Further, the light emitting device 711b includes at least one of an ultraviolet light source, an infrared light source, or a visible light source.

Further, the device chamber further comprises a second device chamber 711c for accommodating the temperature regulating device 711 d. The position of the second device chamber 711c is not limited herein, as long as it can provide a suitable temperature environment for the sample detection chamber, and preferably, in this embodiment, the second device chamber 711c is disposed between the first device chamber 711a and the sample detection chamber 713, and is used to adjust the temperature of the sample detection chamber 713, when a sample is detected, it needs to be ensured that the sample is in a constant temperature condition, and the temperature adjustment device 711d provides a constant temperature environment for the sample detection, so that the detection effect under the constant temperature condition is better. Further, the temperature adjustment device 711d is partially or fully transparent, the temperature adjustment device 711d is disposed between the light emitting device 711b and the sample, and the light source emitted from the light emitting device 711b can be projected onto the sample only when the temperature adjustment device 711d is fully or partially transparent. Further, the temperature adjusting means 711d comprises a temperature sensor and a temperature control unit.

Further, the device chamber further includes a device sealing member 711e, in this embodiment, the device sealing member 711e seals the bottom of the sample detection chamber 713, and in other embodiments, the device sealing member 711e may be disposed at other positions as long as the device sealing member 711e is located between the sample detection chamber 713 and the second device chamber 711c to ensure that the light emitted from the light emitting device 711b can pass through the device sealing member 711e to achieve the effect of assisting in detecting the sample. The device sealing member 711e also seals the device chamber, and if the sample detection chamber 713 passes through the first microchannel 714, the normal operation of each detection device will be affected if the sample flows into the device chamber, so that the device sealing member 711e isolates the sample detection chamber 713 from the device chamber, and the sample can be effectively prevented from leaking. Further, the device sealing member 711e is partially or fully transmissive, and only when the device sealing member 711e is fully or partially transmissive, the light source emitted from the light emitting device 711b can be projected onto the sample.

Referring to fig. 18 to 20, the detection chip body 711 further includes a first chamber lid 711f, and the first chamber lid 711f is used for sealing the sample detection chamber 713, preventing the sample from leaking, and better storing the sample. Further, the first chamber lid 711f is partially transparent or fully transparent, and the light source of the light emitting device 711b can be projected onto the sample only when the first chamber lid 711f is fully transparent or partially transparent. Under the irradiation of the light source, the transparent chamber cover can transmit the related information of the sample so as to analyze the sample.

The detection chip body 711 further includes a second chamber cap 711g, and the second chamber cap 711g is disposed on one side of the second micro flow channel 716 and is used for sealing the second micro flow channel 716. The first chamber lid 711f and the second chamber lid 711g seal the entire detection chip body 711. The second chamber cover 711g serves as a support body to support and accommodate the detection devices, the first chamber cover 711f serves as a cover plate to cover the second chamber cover 711g, and the first chamber cover 711f is detachably connected with the second chamber cover 711 g.

Further, a sample detection chamber 713 is formed between the first chamber lid 711f and the device chamber, the sample detection chamber 713 is used for storing a sample to be detected, the position of the sample detection chamber 713 corresponds to the position of the device chamber, and the shape and the area of the upper bottom surface and the lower bottom surface of the sample detection chamber 713 are also adapted to the shape and the area of the cross section of the device chamber, so that the sample can be detected conveniently. Specifically, in this embodiment, the shape of the sample detection chamber 713 is the same as the shape of the device chamber, and the area size is also the same, so that the light source of the sample detection chamber 713 can completely irradiate the sample in the sample storage chamber, the light source can be fully utilized, and the detection efficiency is higher.

Example 8

Referring to fig. 21 and 22, an embodiment of the invention discloses a microscopic image information collecting module 730, the microscopic image information collecting module 730 includes a microscope body 731, a stage 735, and a microscopic optical information collecting assembly 736, the microscope body 731 includes a lens group 732, a sample to be detected is disposed on the stage 735, the stage 735 is disposed on one side of the lens group 732 where the image is incident, the microscopic optical information collecting assembly 736 is disposed on the microscope body 731, the sample to be detected is magnified by the lens group 732 to form a current microscopic image of the sample to be detected, the current microscopic image of the sample to be detected reflects a current state of the sample to be detected, the microscopic optical information collecting assembly 736 disposed on the microscope body 731 can photograph the current state of the sample to be detected, can extract biological information of the sample to be detected magnified by the microscope and store the biological information in the form of a picture, the picture can be handed over to a detection position to be detected, the detection accuracy is higher, and meanwhile, the picture can be called to perform secondary verification in order to avoid detection errors. The position between the object stage 735, the microscope body 731 and the microscopic optical information acquisition component 736 is relatively fixed, so that a sample to be detected can be directly detected when entering the object stage 735, and the detection is more convenient.

To facilitate understanding of the structure of the microscopic image information acquisition module 730, the microscope body 731, the stage 735, and the microscopic optical information acquisition assembly 736 will be described separately as follows:

referring to fig. 22, the microscope includes a light filtering component 734 and a zooming component 733, the light filtering component 734 is disposed between the microscopic optical information collecting component 736 and the zooming component 733, the light filtering component 734 includes a first filter 734b and a second filter which are sequentially disposed from one end of the microscopic optical information collecting component 736, the first filter 734b and the second filter can select a desired radiation wavelength band to make the sample present an image which is convenient for observation, the zooming component 733 can change focal length within a certain range to obtain different wide and narrow field angles, different size images and different scene ranges, the zooming component 733 can change the shooting range by changing the focal length without changing the shooting distance, thereby being very beneficial to picture composition, in this embodiment, the positions of the light filtering component 734, the zooming component 733 and the light filtering component 734 and the zooming component 733 are relatively fixed, the filter assembly 734, in combination with the zoom assembly 733, can clearly project an image of the sample.

Further, the zoom component 733 includes a first magnifying lens 733a, a second magnifying lens 733b and a protective lens 733c, which are sequentially disposed from one end of the optical filtering component 734, the first magnifying lens 733a and the second magnifying lens 733b provide a magnifying environment for the sample, the second magnifying lens 733b has a fixed position, the sample is stored in the microfluidic detection chip 710, the sample is located between a first focal length and a second focal length of the second magnifying lens 733b, an image of the sample is outside the second focal length of the first magnifying lens 733a and presents an inverted magnified real image, and the second magnifying lens 733b has a fixed position and magnifies the inverted real image of the first magnifying lens 733a and presents a positive false image. The lens protector 733c protects various elements inside the microscope to prevent external dust or impurities from entering the inside of the microscope and contaminating the lenses.

Further, a first magnifying lens 733a, a second magnifying lens 733b, and a protective lens 733c are stacked in center alignment, the first magnifying lens 733a is disposed near the filter assembly 734, the protective lens 733c is disposed at the end of the microscope near the microfluidic detection chip 710, and the second magnifying lens 733b is disposed between the first magnifying lens 733a and the protective lens 733 c. The center-aligned stacking ensures that the relative positions of the first magnifying lens 733a, the second magnifying lens 733b, and the protecting lens 733c are fixed, the magnification is a preset magnification, and the position of the stage 735 also corresponds to the positions of the first magnifying lens 733a, the second magnifying lens 733b, and the protecting lens 733c of the microscope. The sample, the protective lens 733c, the second magnifying lens 733b, and the first magnifying lens 733a are relatively fixed in position, and the sample on the stage 735 can be directly detected without frequently adjusting the positional relationship among the respective portions.

Further, the first magnifying lens 733a is a fresnel lens, and the second magnifying lens 733b is a crescent lens or a fresnel lens. The crescent lens is adopted to detect the sample, the minimum collimation incident light focus can be generated, and the projection effect is good. The Fresnel lens is a screw thread lens, is mostly a sheet formed by injecting and pressing polyolefin materials and is also made of glass, one surface of the lens is a smooth surface, the other surface of the lens is inscribed with concentric circles from small to large, and the texture of the Fresnel lens is designed according to the requirements of light interference and interference, relative sensitivity and receiving angle.

Furthermore, the protective mirror 733c is a plane mirror, the microfluidic detection chip 710 includes a light emitting device 711b, a light source of the light emitting device 711b is directly projected on a sample, the position of the sample corresponds to the position of the microscope, the light source emitted by the light emitting layer is directly projected into the microscope, observation is not facilitated, the capacity of reflecting light by the plane mirror is weak, the plane mirror is arranged at the tail end of the microscope close to the microfluidic detection chip 710, a good illumination environment can be provided, and the imaging effect of the microscope is good.

Referring to fig. 21, further, the micro-optical information collecting component 736 includes a CCD/CMOS integrated component, the CCD integrated component can convert light into charges and store and transfer the charges, and can also take out the stored charges to change the voltage, so that it is an ideal imaging device.

Example 9

Referring to fig. 21 and 23, an embodiment of the present invention discloses a microscopic image collecting module 720, which includes a microfluidic detection chip 710 and the microscopic image information collecting module 730, wherein an object stage 735 is disposed on the microfluidic detection chip 710, and the microfluidic detection chip 710 provides a detection environment for sample detection, such as: light environment and constant temperature environment, in view of the above, the image that microscopic image gathered module 720 gathered can be more clear, more can reflect the relevant information of waiting to detect the sample.

Further, the stage 735 is a sample detection chamber 713 disposed on the microfluidic detection chip 710 for receiving a sample.

Example 10

Referring to fig. 24 and 25, an optical information collecting module 740 is disclosed according to an embodiment of the present invention,

the optical information collection module 740 comprises a microfluidic detection chip 710 and an optical information collection assembly 741, the microfluidic detection chip 710 is configured to accommodate a sample and provide optical information of a detection environment for optical detection of the sample to assist in collecting the optical information of the sample, the microfluidic detection chip 710 can provide a detection environment for optical detection of the sample without a microscope during image collection, and assist in collecting the optical information of the sample, the microfluidic detection chip 710 projects an optical image of the sample into the optical information collection assembly 741, and the optical information collection assembly 741 obtains and stores the optical image information of the sample, so that cost can be saved. In addition, the sample can flow into the microfluidic detection chip 710, the optical information of the sample is directly collected by the optical information collection assembly 740, the sample does not need to be manually transferred and detected, the detection process is simple, and the cost is saved.

To facilitate understanding of the structure of the optical information collection module 740, the microfluidic detection chip 710 and the fluorescence optical information collection assembly 741 will be described separately as follows:

referring to fig. 26 and 27, the microfluidic detection chip 710 according to an embodiment of the present invention includes a detection chip body 711, a detection chip inlet 712, a sample detection chamber 713, and a first micro channel 714, where the sample detection chamber 713 is disposed in the detection chip body 711 and is used for accommodating and assisting a detection sample, the first micro channel 714 is disposed in the detection chip body 711, and the sample flows into the sample detection chamber 713 through the first micro channel 714 from the detection chip inlet 712, in this embodiment, the detection chip inlet 712, the sample detection chamber 713, and the first micro channel 714 are sequentially connected, and the sample can directly flow into the sample detection chamber 713 through the first micro channel 714 for detection, so that the microfluidic detection chip 710 has a high integration level, and the detection process is simple without manual transfer of the detection sample. The microfluidic chip 710 is easy to clean, and a cleaning solution is injected into the first micro-channel 714, and the cleaned sample flows out of the second micro-channel 716, so that the detection chip body 711, the first micro-channel 714 and the second micro-channel 716 can be cleaned more conveniently.

The sample detection chamber 713 may be partially or fully transparent. The sample detection chamber is used for assisting in detecting a sample, the sample detection chamber 713 comprises an upper chamber wall, a lower chamber wall and a side wall, when the side wall is transparent, and the upper chamber wall or the lower chamber wall is also transparent, the sample is introduced into the detection chamber, an external light source can enter the sample detection chamber 713 through the side wall, the transparent upper chamber wall or the lower chamber wall, and light is reflected out of the sample detection chamber 713 through the transparent upper chamber wall or the transparent lower chamber wall, so that a light source environment can be provided for sample detection. When the upper cavity wall and the lower cavity wall are transparent, light provided by the light source can penetrate through the upper cavity wall and the lower cavity wall from one side of the upper cavity wall or the lower cavity wall, and a light environment is provided for sample detection. The microfluidic detection chip 710 in this embodiment is used for assisting the sample to perform detection, has high integration level and simple structure, and reduces the detection cost.

In this embodiment, the first micro channel 714 is disposed in the detection chip body 711, the sample detection chamber 713 is located at one section of the first micro channel 714, the detection chip injection port 712 is disposed at one end of the first micro channel 714 and is communicated with the outside, the sample flows into the first micro channel 714 from the detection chip injection port 712 and directly enters the sample detection chamber 713, the detection module can directly detect the sample in the sample detection chamber 713, the sample detection is not required to be manually transferred, and the detection process is relatively simple.

The detection chip further comprises: a detection chip sample outlet 715 and a second micro-channel 716, wherein the detection chip sample outlet 715 is arranged at one end of the second micro-channel 716 and is communicated with the outside, and the sample flows out of the detection chip sample outlet 715 from the sample detection chamber 713 through the second micro-channel 716. The second micro-channel 716 is disposed in the detection chip body 711, the first micro-channel 714 is communicated with the second micro-channel 716, the sample enters the sample detection chamber 713 from the first micro-channel 714, then flows into the second micro-channel 716 from the sample detection chamber 713, and finally flows out of the detection chip through the detection chip outlet 715, thereby completing the sample detection. In this embodiment, the detection chip includes both the first micro-channel 714 and the second micro-channel 716, if the sample is a urine sample, the sample can be reused by forming a waste liquid flowing out from the second micro-channel 716 after being detected by the first micro-channel 714, and if the sample is a cleaning liquid, the interior of the detection chip can be cleaned for the next use.

The detection chip body 711 further includes a device chamber for accommodating a detection device for providing a detection environment for sample detection.

Further, the device chamber includes a first device chamber 711a, the first device chamber 711a is used for accommodating a light emitting device 711b, the light emitting device 711b emits a light source for detecting the sample, the light source is projected onto the sample, and the sample projected by the light source is transmitted out of the microfluidic detection chip 710, so that the optical sample detection apparatus can detect and analyze the sample. Further, the light emitting device 711b includes at least one of an ultraviolet light source, an infrared light source, or a visible light source.

When the micro-fluidic detection chip 710 is applied to fluorescent image information collection, the light emitting device 711b emits a light source for exciting the fluorescent substance of the sample, the light source is projected onto the sample, and the sample projected by the light source is transmitted out of the micro-fluidic detection chip 710, so that the optical sample detection apparatus can detect and analyze the fluorescent image of the sample. Further, the light emitting device 711b includes at least one of an ultraviolet light source or a blue-violet light source.

When the micro-fluidic detection chip 710 is applied to spectrum information collection, the light emitting device 711b emits a light source for exciting spectrum information of a sample, the light source is projected onto the sample, and the sample projected by the light source is transmitted out of the micro-fluidic detection chip 710, so that the optical sample detection device can detect and analyze the spectrum information of the sample. Further, the light emitting device 711b includes at least one of an infrared light source or an X-ray.

Referring to fig. 18 to 27, the detection chip body 711 further includes a first chamber lid 711f, and the first chamber lid 711f is used for sealing the sample detection chamber 713, preventing the sample from leaking, and better storing the sample. Further, the first chamber lid 711f is partially transparent or fully transparent, and the light source of the light emitting device 711b can be projected onto the sample only when the first chamber lid 711f is fully transparent or partially transparent. Under the irradiation of the light source, the transparent chamber cover can transmit the related information of the sample so as to analyze the sample.

Further, the first chamber lid 711f includes an excitation light filter layer 711h, which is located between the light emitting device 711b and the sample, and the excitation light filter layer 711h is used to filter a spectrum or other light than fluorescence. When the excitation-light filter layer 711h is used to filter out light other than fluorescence, the excitation-light filter layer includes four groups: ultraviolet, violet, blue and green light.

The detection chip body 711 further includes a second chamber cap 711g, and the second chamber cap 711g is disposed on one side of the second micro flow channel 716 and is used for sealing the second micro flow channel 716. The first chamber lid 711f and the second chamber lid 711g seal the entire detection chip body 711. The second chamber cover 711g is used as a support body to support and accommodate various detection devices, the first chamber cover 711f is used as a cover plate 624c to cover the second chamber cover 711g, the first chamber cover 711f is detachably connected with the second chamber cover 711g, specifically, the first chamber cover 711f is connected with the second chamber cover 711g in a clamping, bonding or sliding mode, the first chamber cover 711f is detached, the internal structure of the microfluidic detection chip 710 can be checked, and internal components can be cleaned, maintained or replaced conveniently.

Further, a sample detection chamber 713 is formed between the first chamber lid 711f and the device chamber, the sample detection chamber 713 is used for storing a sample to be detected, the position of the sample detection chamber 713 corresponds to the position of the device chamber, and the shape and the area of the upper bottom surface and the lower bottom surface of the sample detection chamber 713 are also adapted to the shape and the area of the cross section of the device chamber, so that the sample can be detected conveniently. Specifically, in this embodiment, the shape of the sample detection chamber 713 is the same as that of the device chamber, and the area size is also the same, so that the light source of the sample detection chamber 713 can completely irradiate the sample in the sample storage chamber, the light source can be fully utilized, and the detection efficiency is higher.

The optical information collection component 741 includes an image information collection unit and/or a spectrum information collection unit, the image information collection unit is used for collecting sample image information, and the spectrum information collection unit is used for collecting sample spectrum information. The type of the spectrum information collecting unit is not limited as long as the spectrum information of the sample can be received, and in this embodiment, it is preferable that the spectrum information collecting unit includes a light ray and a micro spectrometer, wherein the optical fiber receiving optical path is confocal receiving, that is, the receiving surface and the object surface are conjugate surfaces, so as to implement fixed-point spectrum receiving. One end of the receiving optical fiber is connected to the micro-fluidic detection chip light path, and the other end of the receiving optical fiber is connected to the micro spectrometer, so that spectral information in the object micro region is obtained.

The image information acquisition unit includes at least one of a fluorescence information acquisition module, a microscopic image information acquisition module 730, and an infrared information acquisition module. The microscopic image information acquisition module 730 is used to acquire microscopic image information of the sample, as previously described. The fluorescence information acquisition module and the infrared information acquisition module can acquire fluorescence image information and infrared image information of a sample. The types of the fluorescence information acquisition module and the infrared information acquisition module are not limited as long as sample image information can be acquired, preferably, in the embodiment, the fluorescence information acquisition module and the infrared information acquisition module can be CCD/CMOS integrated assemblies, the CCD integrated assemblies can change light into charges and store and transfer the charges, and can also take out the stored charges to change voltage, so that the CCD integrated assemblies are ideal imaging elements.

The optical information collection component 741 includes a light emitting device, where the light emitting device includes light sources with different wave bands, ultraviolet light, infrared light, and visible light, and provides a light source environment for sample detection. The light emitting device in this embodiment further provides an external light source environment for sample detection, the sample detection chamber is used for assisting in detecting a sample, the sample detection chamber 713 includes an upper chamber wall, a lower chamber wall and a side wall, when the side wall is transparent, and the upper chamber wall or the lower chamber wall is also transparent, the sample is introduced into the detection chamber, the external light source can enter the sample detection chamber 713 through the side wall, the transparent upper chamber wall or the lower chamber wall, and the light is reflected out of the sample detection chamber 713 through the transparent upper chamber wall or the lower chamber wall without passing through the microfluidic detection chip 710.

The optical information acquisition module comprises the microscopic image acquisition module 720, a fluorescence image acquisition module and a spectrum information acquisition module, can be applied to urine detection, and can acquire microscopic images, fluorescence images and spectrum information of urine samples by introducing the urine samples into a sample detection chamber of the microfluidic detection chip. Optical information gathers the module and is not restricted to using in the urine detection field, still can be applied to other human biochemical index detection fields, and preferred, the sample type still includes human body fluid if: serum (plasma), urine, saliva, etc., human tissue such as: epithelial tissue, and mixtures of feces and fluid.

The embodiment provides an optical sample detection device, including aforementioned micro-fluidic detection chip, optical information collection module, when optical sample detection device is used for detecting the urine sample, optical sample detection device is optical urine detection module.

Example 11

Referring to fig. 21, 23, 24 and 3, an embodiment of the present invention discloses a urine detection module disposed on an intelligent toilet for detecting urine, wherein the urine detection module includes a chemical urine detection module and the optical urine detection module, the chemical urine detection module detects chemical components in urine to determine various inorganic substances and organic substances in urine, and performs auxiliary diagnosis and curative effect observation on urinary system diseases, liver and gall diseases, diabetes and other diseases by semi-quantitative and quantitative detection of urine, so as to monitor safe medication and evaluate health status; the optical urine detection module collects images of urine samples, the collected images are sent to a designated analysis position to be analyzed or uploaded to a controller of the intelligent closestool, the controller controls the analysis assembly to analyze the images collected by the optical urine detection module, analysis results of the urine samples are output, and physical conditions of users are judged according to the analysis results.

The optical urine detection module comprises a microscopic image acquisition module 720, a fluorescent image acquisition module and a spectrum information acquisition module. The microscopic image acquisition module 720 uses a microscope to examine the shape and quantity of cells, casts and salt crystal pairs in the urine, the normal urine generally has no red blood cells, white blood cells and epithelial cells, and has no casts, and the increase of the components reflects pathological changes of the urinary system, so that the physical condition of the user can be analyzed. The fluorescence image acquisition module acquires fluorescence information of the urine sample by adopting a fluorescence method, and directly projects the acquired fluorescence information onto the optical information acquisition component 741, so that the optical information acquisition component 741 acquires the fluorescence information of the urine sample. The spectrum information acquisition module can realize real-time acquisition of urine sample images while detecting the spectrum of the urine sample, the spectrum can generate a signal related to an observation position, such as the counting rate of transmitted photons, the photoelectron yield of a total or specific peak, the fluorescence yield and the like, the signals can give various information of elements, chemistry, magnetism and the like, and the physical condition of a user can be analyzed according to the spectrum information.

Example 12

Referring to fig. 28 to 31, an embodiment of the present invention discloses an electrochemical detection chip 760 inserted in a body fluid electrochemical detection module 770 for electrochemically detecting urine, where the electrochemical detection chip 760 includes an insulating substrate 761 and a plurality of chip electrodes 762, where the number of the chip electrodes 762 may be 1 or multiple, and when the number of the chip electrodes 762 is 1, urine drops on the chip electrodes 762, and when the number of the chip electrodes 762 is multiple, the chip electrodes 762 are disposed on the insulating substrate 761 at predetermined intervals. The plurality of chip electrodes 762 form a reaction part 762a and a conductive part 762b on the insulating substrate 761, urine enables the plurality of chip electrodes 762 to be conducted on the reaction part 762a to generate a plurality of electric signals to be transmitted to the conductive part 762b for detection, urine electrochemical indexes are detected through the plurality of electric signals, the relevant conditions of the urine are not judged through vision manually, and detected urine data are more accurate. The reaction part is used for carrying out chemical reaction with urine, the conductive part is used for forming an electric circuit, and the conductive part is not necessarily an electrode per se, and can be a connecting conductor as long as electric signal transmission can be carried out.

To facilitate understanding of the structure of the electrochemical detection chip 760, the insulating substrate 761 and the plurality of chip electrodes 762 will be separately described as follows:

referring to fig. 28 to fig. 31, at least two chip electrodes 762 are provided, urine has conductivity, and when urine drops on at least two chip electrodes 762, the urine is communicated with at least two chip electrodes 762, preferably, the two chip electrodes 762 are taken as an example, the urine is connected with the two chip electrodes 762, the two chip electrodes 762 are positive and negative electrodes, respectively, the conductive parts 762b of the two chip electrodes 762 are connected with the body fluid electrochemical detection module 770, and an electric loop is formed between the two chip electrodes 762, so that an electric signal on the electric loop can be detected. When urine is communicated with the plurality of chip electrodes 762, an electric loop is formed between every two adjacent chip electrodes 762, and by detecting electric signals on the plurality of electric loops, a plurality of groups of data can be compared, and the detected result can be more accurate.

Further, chip electrode 762 is paster chip electrode 762, and paster chip electrode 762 is with low costs, simple to operate, only need with chip electrode 762 paste on insulating substrate 761 can to, the chip electrode 762 after pasting is difficult to drop. Further, when there are a plurality of chip electrodes 762, the chip electrodes 762 should have the same structure and be adhered at predetermined intervals, preferably at equal intervals, so as to ensure the consistency of the basic parameters of the circuit, thereby reducing the detection error.

Further, the insulating substrate 761 is made of an insulating material, and the chip electrode 762 needs to form an electrical circuit and needs to eliminate interference of other non-insulating factors, so that it is necessary to ensure that the insulating substrate 761 is an insulator, and at the same time, the insulating substrate 761 serving as the insulator does not affect data information related to the electrical circuit, and the detection result is more accurate.

Furthermore, the electrochemical index comprises one or more of urine specific gravity, urine pH value, urine protein, uric acid, urine potassium, urine sodium, urine calcium, urine phosphorus, urine sugar and urine chloride, and can be obtained by electric signal detection and analysis. Different detection materials are arranged on the chip electrodes, and the different detection materials can detect the different indexes, and the detection principle is the prior art and is not described herein any more. The chip electrode on the reaction part comprises a detection material or the reaction part comprises a reaction layer, the reaction layer is provided with the detection material, and the detection material can detect the different electrochemical indexes. The urine specific gravity and the urine pH value can be directly detected by the chip electrode, and a reaction layer and a detection material are not required to be arranged.

Referring to fig. 28 to 31, the reaction portion 762a and the conductive portion 762b are disposed on the same side of the insulating substrate 761 or disposed on two opposite sides of the insulating substrate. When the reaction portion and the conductive portion are disposed on two sides of the insulating substrate, the chip electrode 762 bypasses the edge of the insulating substrate 761 from one side of the insulating substrate 761 to the other side, or the chip electrode 762 extends from one side of the insulating substrate 761 to the other side of the insulating substrate 761 through the inside of the insulating substrate 761, so that urine can be prevented from flowing into the conductive portion 762b to cause a short circuit in an electrical circuit, and the detection purpose cannot be achieved. Further, the chip electrodes 762 may bypass the long sides of the insulating substrate 761 or the short sides of the insulating substrate 761, in this embodiment, the chip electrodes 762 preferably bypass the short sides of the insulating substrate 761, the chip electrodes 762 may be laid along the length direction of the insulating substrate 761, the laying distance is longer, the reaction portion 762a is farther away from the conductive portion 762b, and the urine in the reaction portion 762a may be effectively prevented from flowing into the conductive portion 762b, thereby making the detection result of this embodiment more accurate.

Referring to fig. 31, in one embodiment, an isolation structure for protecting the reaction portion 762a and the conductive portion 762b is disposed on the insulating substrate 761.

Specifically, the isolation structure includes a first isolation component 761a, and the first isolation component 761a is used for isolating the reaction part 762a and the conductive part 762b of the substrate from communicating with the outside. The first isolation part 761a is matched with the body fluid electrochemical detection module 770, so that the internal liquid can be effectively prevented from overflowing to the outside, and the closed space is more favorable for cleaning the detection module.

The isolation structure further includes a second isolation part 761b for isolating the reaction part 762a from the conductive part 762 b. In this case, the height of the reaction part 762a should be lower than that of the conductive part 762b, so that urine dropping into the reaction part 762a does not go deep into the conductive part 762b, and the conductive part 762b can be effectively prevented from being contaminated by the urine. Further, the second isolation component 761b has a concave structure, and urine flowing into the reaction portion 762a is collected in the second isolation component 761b and communicated with the chip electrode 762 in the second isolation component 761b, so that the urine does not permeate into the conductive portion 762 b. Further, each of the chip electrodes 762 includes a chip electrode bending portion, and the chip electrode bending portion is adapted to a sidewall of the reaction portion 762a, so that the chip electrodes 762 can be continuously and uninterruptedly disposed on the reaction portion 762a and the conductive portion 762 b. Further, the reaction part 762a is provided with a reaction part liquid outlet 762c, the reaction part liquid outlet 762c extends from the inside of the second partition 761b to the edge of the insulating substrate 761, and the liquid in the reaction part 762a can flow out from the inside of the reaction part 762a to the bottom of the reaction chamber 771c of the detection module through the reaction part liquid outlet 762c, and the liquid does not overflow to the conductive part 762b, so that the conductive part 762b can be effectively protected.

Example 13

Referring to fig. 32 to 39, the present invention discloses a body fluid electrochemical detection module 770 for detecting human urine, which is not limited to the urine detection field, but can also be applied to other human biochemical index detection fields, preferably, the sample types further include human body fluids such as: serum (plasma), urine, saliva, etc., human tissue such as: epithelial tissue, and mixtures of feces and fluid. The body fluid electrochemical detection module 770 comprises a detection module body 771, an electrochemical sample inlet 772 and a plurality of connecting electrodes 773, the detection module body 771 comprises a reaction zone 771a and a connecting zone 771b, the plurality of connecting electrodes 773 are arranged on the connecting zone 771b at preset intervals, the plurality of connecting electrodes 773 are used for forming an electrochemical reaction loop, the number of the connecting electrodes 773 can be 1 or more, when the number of the connecting electrodes 773 is 1, urine drops on the chip electrodes 762, the chip electrodes 762 are connected to the connecting electrode 773 to form an electric circuit, when the number of the connecting electrodes 773 is multiple, the connecting electrodes 773 are disposed on the connecting area 771b at predetermined intervals, liquid enters the reaction area 771a from the electrochemical sample inlet 772, the liquid includes urine, the urine enters the reaction area 771a and then undergoes an electrochemical reaction, and the connecting electrodes 773 can detect various data of the urine, so that the accuracy of urine detection can be improved.

To facilitate understanding of the structure of the body fluid electrochemical detection module 770, the components of the detection module will now be described separately as follows:

referring to fig. 32 to fig. 35, the detection module further includes an electrochemical sample outlet 774, and the electrochemical sample outlet 774 is used for flowing out the liquid. When the liquid is urine, the detected waste liquid flows from the electrochemical sample outlet 774 to the waste liquid pool, and when the liquid is cleaning liquid, the cleaned cleaning liquid also flows from the electrochemical sample outlet 774 to the waste liquid pool. Further, the detection module further comprises a sample outlet tube 776, the electrochemical sample outlet 774 is arranged at one end of the sample inlet tube 775, the other end of the sample inlet tube 775 is arranged at the reaction zone 771a, and liquid from the sample inlet tube 775 is discharged out of the detection module body 771 through the electrochemical sample outlet 774 through the sample outlet tube 776.

The body fluid electrochemical detection module 770 further comprises a sample inlet channel 775, and the liquid enters the reaction area 771a through the electrochemical sample inlet 772 via the sample inlet channel 775. The liquid flowing into the sample introduction pipeline 775 comprises a mixed liquid of urine and a reagent, and a micro-flow pump is arranged on the sample introduction pipeline 775, so that a liquid sample can be quantitatively obtained.

Furthermore, the sample inlet pipeline 775 comprises a sample inlet pipeline liquid outlet 775b, the sample inlet pipeline liquid outlet 775b is right opposite to the reaction area 771a, liquid flowing from the sample inlet pipeline 775 to the reaction area 771a can be uniformly diffused, and measured data are more accurate. Sample introduction pipeline 775 still includes sample introduction pipeline body 775a, sample introduction pipeline body 775a sets up along detecting module length direction, sample introduction pipeline 775 still includes bending portion 775c, sample introduction pipeline liquid outlet 775b sets up at the end of bending portion 775c, the head end of bending portion 775c is connected with sample introduction pipeline body 775a, bending portion 775c bends the angle and is 90 to make parallel arrangement's sample introduction pipeline 775's second liquid outlet just to reaction zone 771 a.

Furthermore, the sample inlet pipes 775 are integrated on the detection module body 771, and the sample inlet pipes 775 can be integrated on the side wall, the top wall or the inside of the module body, so that the sample inlet pipes 775 can be supported, and the sample inlet pipes 775 can be prevented from sliding to cause the dislocation of the liquid outlet ports 775b of the sample inlet pipes.

Referring to fig. 34 to 39, further, the reaction area 771a includes a reaction cavity 771c, the sample outlet tube 776 is communicated with the reaction cavity 771c, the reaction cavity 771c is used for accommodating liquid, specifically, for accommodating a sample to be detected and waste liquid, the sample outlet tube 776 is used for discharging the waste liquid out of the detection module body 771, the reaction part 762a of the detection chip is located in the reaction cavity 771c, the reaction part 762a can only accommodate a small amount of liquid flowing from the sample inlet tube 775, and redundant liquid can flow into the bottom of the reaction cavity 771c from the reaction part 762a and become waste liquid.

The reaction chamber 771c includes a chamber 771d and a sealing structure 771e, the sealing structure 771e is used for sealing the chamber 771d, so that the reaction chamber 771c becomes the sealed chamber 771d, liquid flows into the reaction chamber 771c and then directly flows out of the sample outlet pipe 776, the liquid cannot be accumulated and overflows the reaction chamber 771c, and the liquid cannot be remained in the detection module body 771. Furthermore, a sample introduction pipeline mounting hole 711g is formed in the sealing structure 771e, the sample introduction pipeline 775 is communicated with the reaction cavity 771c through the sample introduction pipeline mounting hole 711g, and the sample introduction pipeline 775 is hermetically connected with the sealing structure 771e through the sample introduction pipeline mounting hole 711 g.

Further, referring to fig. 35 to fig. 36, a liquid collecting groove 771f is disposed at the bottom of the reaction chamber 771c, the liquid collecting groove 771f is communicated with the sample outlet tube 776, and the liquid collecting groove 771f is used for collecting the liquid in the reaction area 771a into the sample outlet tube 776. The lowest height of the liquid collecting groove 771f is not lower than that of the sample outlet pipe 776, the side wall of the liquid collecting groove 771f is an arc surface or an inclined surface, the width of the bottom of the liquid collecting groove 771f is smaller than that of the top of the liquid collecting groove 771f, and liquid in the reaction area 771a can be converged to the bottom of the liquid collecting groove 771f along the side wall of the liquid collecting groove 771f and then flows into the sample outlet pipe 776 from the bottom.

The liquid is a urine sample or a cleaning solution. When liquid is a urine sample, the urine sample flows into the reaction part 762a of the detection chip from the sample introduction pipeline 775 and forms an electric loop, the detection module detects parameters of the urine sample through the electric loop, redundant urine flows into the reaction cavity 771c from the reaction part 762a, waste liquid in the reaction cavity 771c is gathered in the liquid collection groove 771f and flows into the sample discharge pipeline 776, and finally flows out of the detection module. When liquid is the cleaning solution, liquid is cleaned the detection module, and the cleaning solution is cleaned the reaction part 762a of detecting the chip from advancing the sample pipeline 775 inflow reaction area 771a, and unnecessary cleaning solution and the waste liquid after the cleaning flow into to reaction chamber 771c in, clean cavity 771d lateral wall and collecting tank 771f, and the waste liquid after the cleaning flows out the detection module from play appearance pipeline 776.

Example 14

Referring to fig. 38 and 39, the present invention discloses an electrochemical body fluid testing device 750, wherein the electrochemical body fluid testing device 750 includes an electrochemical testing chip 760 and the body fluid electrochemical testing module 770, the testing module includes a connection region 771b and a reaction region 771a, the testing chip includes a reaction part 762a, a conductive part 762b and an isolation structure, the connection region 771b corresponds to the conductive part b, the reaction region 771a corresponds to the reaction part 762a, the conductive part 762b is detachably inserted into the connection region 771b, an electrical circuit is formed on the testing module at the reaction region 771a and the urine flows into the reaction part 762a, the electrical conductivity of urine is calculated by testing the current value on the electrical circuit, the testing environment of the present invention is wide, when data of a plurality of urine samples needs to be tested, the testing module can be quickly and conveniently inserted and pulled out to replace the types of the testing module, different types of testing modules have different electrodes, different electrodes form different electric loops, current values on the different electric loops are different, finally measured conductivity is different, accordingly, comparison analysis can be conducted on the reference value of the to-be-detected material input in advance, different urine sample data can be obtained, the urine sample data can be analyzed in a conductivity detection mode, and the obtained detection result is more accurate. Simultaneously, the detection module can used repeatedly, can reduce and detect the cost. Meanwhile, the isolation structure may effectively protect the reaction part 762a and the conductive part 762 b.

Further, the detection module includes a sealing structure 771e, and the sealing structure 771e is used for cooperating with the isolation structure to seal the reaction part 762a and the reaction area 771 a. The sealing structure 771e can seal the reaction part 762a and the reaction zone 771a, and then clean the reaction part 762a and the reaction zone 771 a.

Further, the connection electrode 773 of the connection region 771b is provided corresponding to the chip electrode 762 of the conductive portion 762b, and the reaction region 771a is provided corresponding to the reaction portion 762 a.

Example 15

Referring to fig. 40, an embodiment of the present invention discloses a system for rapidly detecting biochemical indicators of a human body, including a sampling device, a sample introduction device and a detection device, wherein the sampling device collects samples required for detecting biochemical indicators of a human body, the sample introduction device is configured to transmit the collected samples to a designated location for detection, the detection device is configured to detect the samples and obtain biochemical indicator information of the human body, and the detection device at least includes: the optical detection module and the chemical detection module, sampling device carry the sample respectively to the optical detection module and the chemical detection module, and the optical detection module can detect the optical information of sample, and the chemical detection module can detect the chemical information of sample, combines the optical information and the chemical information of sample, can detect out human biochemical index information more comprehensively, and the index information who detects out is also more accurate.

Preferably, the types of samples that can be detected by the rapid human biochemical indicator detection system mainly include human body fluids such as: serum (plasma), urine, saliva, etc., and human tissues such as: epithelial tissue.

Preferably, in this embodiment, the body fluid is preferably urine, and the sampling device includes the urine sampler and the urine sampling head for sampling the urine sample.

Preferably, the sample introduction device comprises a urine transmission pipeline, and the urine transmission pipeline transmits the urine sample collected by the urine sampler to the detection device for urine detection.

Preferably, optics detects the module and includes aforementioned optics urine detection module, and optics urine detection module can detect the optical information of urine sample, and chemistry detects the module and includes aforementioned chemistry urine detection module, and chemistry detection module can detect the chemical information of urine sample.

Preferably, the optical detection module comprises: and the microscopic detection module is used for acquiring a microscopic image of the sample. The microscopic detection module comprises the microscopic image acquisition module and is used for carrying out urine detection by adopting a urine detection method based on the microscopic image according to the acquired microscopic image of the urine sample.

Preferably, the optical detection module comprises: and the fluorescence detection module is used for acquiring images of the samples after the samples are excited by fluorescence. The fluorescence detection module comprises the fluorescence image acquisition module and is used for carrying out urine detection by adopting a fluorescence reagent-based urine component detection method which is described later according to an acquired image of the urine sample after being excited by fluorescence.

Preferably, the optical detection module comprises: and the spectrum detection module is used for detecting the spectrum information of the sample. The spectrum detection module comprises the spectrum information acquisition module and is used for detecting urine by adopting a spectrum detection method of urine components according to the detected spectrum information of the urine sample.

Preferably, the chemical detection module comprises: and the electrochemical detection module is used for detecting the electric signal change information of the sample. The electrochemical detection module comprises the electrochemical body fluid detection device and is used for detecting urine by adopting a urine electrochemical detection method which is described later according to the change information of the electric signals of the detected urine sample.

Preferably, the sample introduction device comprises: and the microflow pump is used for temporarily storing the sample and quantitatively transmitting the sample.

Preferably, the system further comprises: the cleaning device is used for cleaning the human body biochemical index rapid detection system. In one embodiment, the system itself can be used as a cleaning system, for example, a cleaning solution is introduced into the urine electrochemical detection module, and the cleaning solution becomes a waste solution after the cleaning process and flows out of the urine electrochemical detection module.

Preferably, the system further comprises: and the driving device is used for driving the rapid detection system for the human body biochemical indexes to work. The driving device can be a stepping motor and drives each part of the human body biochemical index rapid detection system to operate.

Preferably, the system further comprises: and the control device is used for controlling the rapid detection system for the human body biochemical indexes. The control device can be a central processing unit and controls the sampling device to sample, the sampling device is controlled to transmit the collected sample to the position of the detection device to detect, and the detection device is controlled to detect.

Example 16

Referring to fig. 41 to 43, an embodiment of the present invention discloses a urine detection method based on microscopic images, including:

s100, injecting urine into a sample detection chamber;

the sample detection chamber is positioned in the microfluidic detection chip, the position of the sample detection chamber is fixed in the detection process, the sample detection chamber is relatively fixed with the microscope body and the position between the sample detection chamber and the microscopic optical information acquisition assembly, urine can be directly injected into the sample detection chamber, the urine waits to be detected after entering the sample detection chamber, the placement position of the urine sample does not need to be adjusted manually, and the detection flow is simple.

S110, controlling light of a background light source to enter a sample detection chamber through the wall of the sample detection chamber;

the sample detection chamber comprises an upper chamber wall, a lower chamber wall and a side wall, when the side wall is transparent, the upper chamber wall or the lower chamber wall is also transparent, a sample is introduced into the detection chamber, an external background light source can enter the sample detection chamber through the side wall, the transparent upper chamber wall or the lower chamber wall, and light is reflected out of the sample detection chamber through the transparent upper chamber wall or the lower chamber wall, so that a light source environment can be provided for sample detection. When the upper cavity wall and the lower cavity wall are transparent, light provided by the light source can penetrate through the upper cavity wall and the lower cavity wall from one side of the upper cavity wall or the lower cavity wall, and a light environment is provided for sample detection. The background light source arranged in the step can meet the requirements of different detection environments.

S120, collecting microscopic image information of urine through the sample detection chamber;

the micro-optical information acquisition assembly of the embodiment is arranged on one side of the microscope body and can acquire micro-image information from the microscope body. The microscopic optical information acquisition assembly can store the microscopic image information of the acquired urine sample and transfer the image information to a detection position for detection, so that the detection accuracy is higher. In addition, in order to avoid detection errors, the image information can be called to carry out secondary verification.

S130, obtaining a microscopic image of the urine sample;

and (3) shooting the urine microscopic image by using the microscopic optical information acquisition assembly, storing the shot microscopic image and sending the stored microscopic image to a control system, and analyzing and processing the urine microscopic image.

S140, preliminarily classifying the urinary sediment through a neural network algorithm;

in this step, the type of the neural network algorithm is not limited, and at least one of the neural network algorithms such as the Convolutional Neural Network (CNN), the Recurrent Neural Network (RNN), and the generation countermeasure network (GAN) may be selected, and preferably, in this embodiment, the present invention employs the Convolutional Neural Network (CNN). The urinary sediment is a tangible component in urine, is sediment formed after the urine is centrifuged, and is also a combination of the quality and the quantity of the tangible component in the urine, the urinary sediment comprises various tangible components such as cells, casts, crystals, bacteria, sperms and the like, and the content of each component in the urine needs to be determined when the urine is detected, so the urinary sediment needs to be preliminarily classified.

S141, preliminarily classifying the urinary sediments according to casts, cells, crystals, bacteria and sperms;

the network structure of the CNN comprises a convolution layer, a sampling layer and a full connection layer, wherein each layer is generally distributed with a plurality of independent neurons which are connected with each other to form a two-dimensional plane, so that the CNN has good performance in identifying two-dimensional shapes. The new form of network structure can keep unchanged when the image to be recognized is scaled, translated and tilted, and has stronger adaptability to image deformation. In the supervised mode, because a large number of training samples are required and a connection is established between the training samples and the test samples, the CNN adopts the supervised training mode.

CNN is adopted to carry out primary classification on the urinary sediments according to tube types, cells, crystals, bacteria and sperms, and the convolution and sampling process mainly comprises feature extraction, feature mapping and sub-sampling:

s142, counting bacteria and sperms respectively;

counting bacteria and sperms, wherein the number of the bacteria reflects the urinary tract infection condition, and the more the bacteria are, the more the urinary tract infection condition is serious; the number of spermatozoa reflects the health of the human reproductive system.

S150, identifying the preliminarily classified urine sediments through image processing and calculating morphological parameters and gray statistical parameters of the urine sediments;

in this step, the morphological parameters and the gray statistical parameters reflect the image characteristics of various urine sediments, and the types of the various urine sediments can be determined by calculating the morphological parameters and the gray statistical parameters of the various urine sediments.

Referring to fig. 43, the step S150 includes:

s151, carrying out image denoising treatment on the preliminarily classified urine sediment images;

the method has the advantages of removing some irrelevant information such as noise of the urine sediment images, increasing the contrast, improving the image quality and clearly separating the foreground and the background of the urine sediment images. The denoising method is not limited herein, and may be gaussian low-pass filtering, bilateral filtering denoising, non-local mean denoising, kernel regression for image denoising, and the like.

A two-dimensional gaussian function is as follows:

where (x, y) is a point coordinate, which may be considered an integer in image processing; σ is the standard deviation. To obtain a template of a gaussian filter, the gaussian function may be discretized, and the obtained gaussian function value is used as a coefficient of the template, and then applied to an image for image processing.

S152, carrying out image enhancement processing on the urine sediment image subjected to image denoising processing;

the image enhancement process can correct the effects of uneven lighting on the images of urinary sediment.

The top-hat transform of a gray-scale image f is defined as f minus its on operation:

the bottom-hat transform of a gray-scale image f is defined as the f-close operation minus f:

B_hat(f)＝(f·b)-f

indicating an on operation of the result element on the target image, and (f · b) indicating an off operation on the target element.

Therefore, the top-cap-bottom-cap transformation is then:

TB_hat＝f+T_hat-B_hat

s153, carrying out image segmentation on the urinary sediment image subjected to image enhancement;

in this step, an image edge algorithm is used for image segmentation processing, but the processing method is not limited to the image edge algorithm, and may be a majority of image segmentation algorithms, an image threshold segmentation algorithm, a region-based segmentation algorithm, a morphological watershed algorithm, and the like. An edge is a collection of pixels with abrupt changes in gray levels in an image and is typically detected using differentiation. The edge detection algorithm has: the method comprises the following steps of selecting a Roberts operator, a Prewitt operator, a Sobel operator, a Marr-Hilderth edge detection algorithm, a Canny edge detection algorithm and the like, wherein in the step, the Canny edge detection algorithm is selected.

a) And calculating the gradient strength and the direction of each pixel point in the image.

In the image, the degree and direction of change in the gray-scale value are expressed by gradients. It can get gradient values in different directions by dot-multiplying a sobel or other operators: g_x(m，n)，g_y(m, n), calculating a gradient value and a gradient direction by the following formula for the integrated gradient:

where (m, n) is a point coordinate, which can be considered an integer in image processing.

b) Non-Maximum Suppression (Non-Maximum Suppression) is applied to eliminate spurious responses due to edge detection. The width of the edge is made to be 1 pixel point as much as possible: if a pixel belongs to the edge, the gradient value of the pixel in the gradient direction is the largest. Otherwise, it is not an edge, and the gray value is set to 0.

c) A Double-Threshold (Double-Threshold) detection is applied to determine true and potential edges. Two thresholds (threshold) are set, maxVal and minVal respectively. Where all above maxVal are detected as edges and all below minval are detected as non-edges. For the middle pixel point, if the middle pixel point is adjacent to the pixel point determined as the edge, the edge is determined; otherwise, it is not edge.

d) Edge detection is finally accomplished by suppressing isolated weak edges.

S154, identifying the shape characteristics of various urine sediments;

and s155, calculating the morphological parameters and the gray statistical parameters of various urine sediments.

The morphological parameters are calculated on the basis of the binary image taken by the microscope, mainly in order to obtain morphological information as 5 sets of characteristic values. The morphological parameters include: area s, perimeter L, circularity c, squareness R, and contour fit error.

a) Area S

The area s is the number of pixels in the target region and is related to the boundary of the target.

Wherein, p and q are respectively the maximum values of the horizontal direction and the vertical direction of the region, and R is the target region.

b) Circumference L

The perimeter L is the sum of all pixels on the boundary of the target area. The mathematical expression is as follows:

p_t＝T_i(mod)2

where N represents the total number of pixels of the contour and Ti represents the number of strands tracing the contour of the cell in a counterclockwise direction from point i to the next.

c) Circularity C

The image circularity C represents the degree that the shape of the target image is close to a circle, and is a comprehensive measure representing parameter of the area shape, and the mathematical expression of the comprehensive measure representing parameter is as follows:

when C is 1, it indicates that the target image shape is a circle, and as the C value increases, it indicates that the target image shape deviates from the circle.

d) Rectangular degree R

The image squareness degree R is the deviation degree of the product of the area of the target image contour and the target height width, and the mathematical expression is as follows:

where W denotes the width of the circumscribed rectangle of the target image, H denotes the height of the circumscribed rectangle of the target image, and when the target region is a rectangle, R is 1.

e) Fitting error:

the fitting error is the distance error between a point on the region contour and a corresponding point on the fitting curve, and can be represented by the average distance between the region boundary and the corresponding point pair of the fitting curve, and the calculation formula is shown as the following formula.

Wherein N is the number of pixel points on the contour, (x)_k,y_k) Indicating a point on the contour, (u)_k,y_k) Is (x)_k,y_k) The symbol | is used to determine the distance between two points, corresponding to the points on the fitted curve. Obviously, the smaller the fitting error, the more the fitted curve fits to the target boundary, and the closer the cell is to a circle or ellipse.

The gray scale statistical characteristics are mainly calculated based on a microscopic cell image gray scale histogram, and characteristic parameters extracted by the gray scale statistical characteristics comprise an average value m; a variance σ; third moment μ _ 3; and consistency U.

Wherein L represents the number of gray levels of a gray image, z_iRepresenting random gray values, p (z)_i) A histogram representing a region.

f) Average value m

The average value m represents the average gray value of a certain target area of the image, and the mathematical expression is as follows:

g) variance σ

The variance σ represents the dispersion degree of the gray level in a certain target area of the image, and the mathematical expression of the variance σ is as follows:

h) third moment mu₃

Third moment mu₃The degree of symmetry of the image gray level histogram is reflected, and the mathematical expression is as follows:

i) consistency U

The consistency U reflects the degree of dispersion of the gray value distribution in a certain region, and the mathematical expression thereof is:

s160, carrying out secondary classification on various urine sediments through an interpretable machine learning algorithm according to morphological parameters and gray statistical parameters of the various urine sediments;

the interpretable machine learning algorithm includes one of a LightGBM classification algorithm, a logistic regression algorithm, an SVM algorithm, a random forest algorithm, a KNN algorithm, and a bayesian algorithm. In the present embodiment, the type of classification algorithm employed is not limited, and preferably, the LightGBM classification algorithm is employed.

Specifically, step S7 includes:

s161, classifying the target, calculating morphological parameters and calculating gray statistics into a training set and a testing set, constructing a LightGBM classification model by using the training set as an input variable, and optimizing parameters of the LightGBM classification model by using a grid search method to obtain the optimized LightGBM classification model.

And S162, training the optimized LightGBM classification model by utilizing the test set to obtain the trained LightGBM classification model.

And S163, outputting type subdivision through the model.

The secondary classification of the various types of urinary sediment is a subdivision of casts, cells and crystals.

Casts are important constituents of urinary sediments, and the presence of casts indicates substantial damage to the kidney, which represents damage to the glomeruli or tubules.

The tube types are classified into the following categories:

1) a transparent tube type; 2) a cell cast; 3) a granular tube type; 4) a wax-like tube; 5) a fat tube type; 6) a mixed tube type; 7) wide tube type.

Cells are classified into the following categories:

1) red blood cells; 2) (ii) a leukocyte; 3) squamous epithelial cells; 4) non-squamous epithelial cells; 5) phagocytic cells; 6) and (3) heterologous cells.

The crystallization is classified into the following categories:

1) calcium oxalate crystallization; 2) crystallizing uric acid; 3) crystallizing phosphate; 4) and (4) crystallizing the medicine.

S170, counting the urine sediments after secondary classification to obtain a counting result;

and S180, obtaining a urine detection result according to the counting result of each urine sediment.

Example 17

The present embodiment discloses a method for detecting urine components based on a fluorescent reagent, please refer to fig. 44 and fig. 45, which includes the following steps:

s200, injecting urine and a fluorescent reagent into a sample detection chamber;

in this step, the urine may be injected first and then the fluorescent reagent may be injected, or the fluorescent reagent may be injected first and then the urine may be injected, or the urine and the fluorescent reagent may be mixed first and then the mixed solution of the urine and the fluorescent reagent may be injected.

The sample detection chamber is positioned in the microfluidic detection chip, the position of the sample detection chamber is fixed in the detection process, urine can be directly injected into the sample detection chamber, the urine waits to be detected after entering the sample detection chamber, the placement position of the urine sample does not need to be adjusted manually, and the detection process is simple.

Before step S200, the method further includes:

s201, defoaming the urine.

Some air bubbles are usually present in urine, and the presence of air bubbles affects the amount of urine sampled and the detection effect, and therefore, a defoaming process is required.

In this embodiment, the defoaming treatment of urine is mainly performed by a precipitation defoaming method, but the defoaming method is not limited thereto, and defoaming methods such as a chemical defoaming method and a physical defoaming method may be used.

S210, a fluorescent light source emits fluorescent reagent exciting light to the sample detection chamber, and mixed liquid of the urine and the fluorescent reagent is excited to generate fluorescence;

the mixed liquid of urine and the fluorescent reagent contains fluorescent substances which can be excited to generate fluorescence, and the mixed liquid can emit the fluorescence when the fluorescent light source irradiates the fluorescent substances.

Step S210 includes:

s211, filtering light emitted by the fluorescent light source, and exciting the mixed liquid of the urine and the fluorescent reagent to generate fluorescence.

In step S211, only the fluorescent light source capable of exciting the fluorescent substance is left as a result of the filtering process, so as to avoid the influence of other light sources on the excitation effect when exciting the fluorescent substance.

S220, collecting the mixed liquid fluorescence image excited by the fluorescence light source through the sample detection chamber.

The sample detection chamber is partially or completely transparent, and the fluorescence image of the mixed liquid can penetrate through and be emitted out of the sample detection chamber.

The optical information acquisition assembly of the embodiment is arranged on one side of the sample detection chamber and can acquire fluorescence image information from the sample detection chamber. The optical information acquisition assembly can store the acquired fluorescence image information of the mixed liquid and transfer the image information to a detection position for detection, and the detection accuracy is higher. In addition, in order to avoid detection errors, the image information can be called to carry out secondary verification.

Before step S220, the method further includes:

and S221, filtering the light transmitted through the sample detection chamber to leave the fluorescence generated by the mixed solution.

Step S221 has a denoising function, and although only a light source capable of exciting a fluorescent substance remains after the fluorescent light source is filtered for the first time, the light source can generate light rays with different wavelength bands, the light rays with different wavelength bands can penetrate through the sample detection chamber, but only the light rays with a wavelength band within a specified range can excite the fluorescent substance, and the light rays with the remaining wavelength bands can be emitted from the sample detection chamber along with the excited fluorescent light, so that the excited fluorescent light has certain interference, and in order to improve the detection effect, the filtering process needs to be performed in this step.

The step S220 includes:

and S222, filtering the ambient light on the fluorescence transmission path before collection.

In the foregoing steps S221 and S222, although the interference of the fluorescent light source itself is eliminated, the interference of the external ambient light on the urine detection cannot be eliminated, so that the ambient light on the fluorescent transmission path is filtered before the fluorescent image is collected, and accordingly, the collected fluorescent image is more accurate, and the finally obtained urine detection result is more accurate.

Further included after step S220 is:

and S223, cleaning the detection device.

The urine has certain peculiar smell, if not handling, the peculiar smell still can aggravate, influences the air circumstance, simultaneously, if do not carry out cleaning, remaining urine can influence next urine and detect. Detection device relates to all devices in the whole testing process, mainly relates to and cleans urine sample, urine transmission, urine detection relevant device, in this embodiment, specifically is to carry out cleaning to urine sample, sample microflow pump, urine transmission pipeline and urine detection module.

After step S220, the present invention further includes the steps of:

s230, acquiring a urine collection image of the urine sample added with the fluorescent reagent after fluorescence excitation;

s240, inputting the urine collecting image into a preset neural network model;

for analyzing the image data of the urine sample, the urine collected image of the urine sample after fluorescence excitation needs to be used as an input original image, and the input original image is input into a neural network model with preset values. The neural network model includes fast R-CNN, SSD, YOLO, and the like, and in this embodiment, the kind of use is not limited, and preferably, the fast R-CNN model is selected for identification in this step.

The step of identifying by using the Faster R-CNN model comprises the following steps:

the method selects a relatively simple ResNet50 network to replace a VGG16 network used by the original network, because the ResNet50 network uses a structure of a residual block, the problem of gradient disappearance caused by deepening of the network layer number can be effectively prevented, and in addition, the operation time of ResNet50 in the ResNet series network is short, so that the ResNet50 network is finally selected as a feature extractor of the network. The size of the input network is set to 640 x 640, the sub image block size does not satisfy 640, and 0 is used to fill the sub image edge to adjust to the input size. After the image is input into the feature extraction layer, the ResNet50 network performs feature extraction in five stages consisting of different numbers of convolution layer combinations, batch normalization layers, ReLU activation layers and maximum pooling layers, and a residual block structure realized by short links is used for learning residual errors. In order to intuitively explain the effect of the feature extraction layer, the feature maps output by the five feature layers of the ResNet50 are visualized. The network structure of the ResNet50 and the visualization effects of feature maps C1 to C5 show the 1 st feature layer to the 5 th feature layer, each feature layer is obtained by downsampling upper layer data, and since the input size is fixed to 640 × 640, the sizes of C1 to C5 are: [320,320], [160,160], [80,80], [40,40], and [20,20 ].

S250, weighting the blocked cell image in the urine collection image in each layer of convolutional neural network of the neural network model;

in the urine collected image, occlusion may exist among cells, the occluded cells are difficult to be found by a neural network model, and the urine collected image needs to be weighted.

Step S250 includes: and S251, embedding an attention mechanism in each layer of convolutional neural network.

In order to solve the problem of shielding among cells, an attention mechanism is embedded into each layer of convolutional neural network in the step, and the shielded cells in each layer of convolutional neural network are weighted so as to enhance the receptive field of a feature extraction layer of the convolutional neural network and improve the performance of a neural network model.

S260, carrying out cell omission prevention identification processing on the weighted urine collection image:

the attention mechanism includes the following:

the urine collection image input into the convolutional neural network is subjected to feature extraction after passing through a feature extraction layer of the convolutional neural network, so that a feature map with the shape of H multiplied by W multiplied by C is obtained, wherein the size of the feature map is represented by H multiplied by W, and C is the number of channels.

Compression (sequeze): feature compression is performed along the spatial dimension, each two-dimensional feature channel is changed into a real number which has a global receptive field to some extent, and the output dimension is matched with the number of input feature channels. It characterizes the global distribution of responses over the eigen-channels and makes it possible to obtain a global receptive field also for layers close to the input. The specific operation is to perform global pooling processing (global pooling processing) on the original feature map C W H, and then obtain a feature map with the size of 1x 1C, which has a global receptive field.

Activation (activation): the output 1x1xC feature map is processed by two fully connected neural networks, and finally, a mechanism similar to a gate in a recurrent neural network is used for generating weight for each feature channel through parameters.

Characteristic re-calibration: and using the result obtained by the Excitation as a weight, then weighting the weight to C channels of U channel by channel through multiplication, completing the recalibration of the original characteristics on the channel dimension, and using the recalibrated original characteristics as input data of the next stage.

Step S260 further includes: and S261, performing feature fusion treatment on the cells with different sizes.

In the urine collection image, not only the problem of blocking of cells but also the problem of uneven size of fluorescent cells are caused, and undersized cells and blocked cells are likely to be omitted, so that feature fusion processing needs to be performed on cells with different sizes, and the feature fusion processing is mainly used for identifying a boundary frame of each cell in the urine collection image.

In the embodiment, a Feature Pyramid Network (FPN) is fused into the Faster R-CNN, so that the cognition of a detector on the whole graph information is increased.

1) Firstly, urine collection images are sent into a fused underlying network, and a five-stage characteristic diagram is obtained through a network combining a ResNet50 and an attention mechanism structure.

2) The C1-C5 layers are the characteristic layers obtained by the characteristic extraction network, and then the dimension reduction is carried out on the C4 layer by 1 × 1 convolution, so that the number of characteristic channels of C4 is matched with the number of characteristic channels of P5; after the P5 is upsampled, the P5 and the C4 feature map size are consistent, and finally the two are added to obtain a fused layer P4, and the rest layers are repeated.

3) Then, RPN training is performed on the obtained P2 to P6 layers (top down network, core of FPN) to obtain candidate regions (region pro-usal), and then the classification layer and the regression layer are connected after convolution by 3 × 3 as in the operation of the original fast R CNN. P2-P5 are used to predict the bounding box of cells, P6 is used in RPN networks.

S270, improving the identification region suggestion box of the cell.

Inputting the feature map output by the FPN structure into an RPN network layer, and forming a certain number of prior frames according to the feature map in a sliding window mode. The original Faster R CNN sets three prior boxes with the aspect ratio of (1:2,1:1,2:1), and the prior boxes with the three ratios can adapt to objects with different shapes and sizes in the COCO data set. To adapt the model to the characteristics of the cell, the initial aspect ratio of the generated prior frame is adjusted to (1:2,4:5,1:1,2: 1). And the initial size of the prior frame is set to (16,32,64,128,256), 20 prior frames are generated for each point on the feature map, and a total of W × H × 20 prior frames are generated on the picture with the size of W × H. The prior box is then binned, the method of classification being based on IoU threshold classification. Classifying prior frames with IoU greater than 0.8 from the true frames of any sugarcane seedling as foreground; the prior box with IoU values less than 0.2 for all real boxes is classified as background. IoU the calculation formula is as follows:

example 18

Referring to fig. 46, the embodiment of the present invention discloses a method for detecting a urine component by a spectrum, wherein the detection of the urine component by the spectrum according to the present invention includes at least one of fourier infrared spectrum detection, raman spectrum detection, fluorescence spectrum detection and ultraviolet spectrum detection.

The spectrum detection method comprises the following steps:

s300, injecting urine into a sample detection chamber;

Before the step S300, the present invention further includes:

s301, injecting a spectrum detection reagent into the sample detection chamber.

In this step, the urine may be injected first and then the spectrum detection reagent, or the spectrum detection reagent may be injected first and then the urine may be injected, or the urine and the spectrum detection reagent may be mixed first and then the mixed solution of the urine and the spectrum detection reagent may be injected.

After the step S300, the present invention further includes:

s302, adjusting the temperature of urine in the sample detection chamber;

when detecting the urine sample, need guarantee that the urine sample is in a temperature environment suitable relatively, when urine sample external environment is undercooled or overheated, external environment temperature can influence the temperature of urine sample and finally can influence the detection effect of urine, consequently, need adjust the temperature of urine in the sample detection cavity.

S310, controlling light of a background light source to enter the sample detection chamber through the cavity wall of the sample detection chamber;

Before the step of S310, the present invention further includes:

s311, filtering the light of the background light source to obtain light within a preset wave band range;

step S311 has a denoising function, the background light source can generate light beams of different wavelength bands, but only light beams within a preset wavelength band range can penetrate through the sample detection chamber to form spectral information, light beams of other wavelength bands have certain interference on the process of forming spectral information on the urine sample, and light beams of other wavelength bands can be emitted from the sample detection chamber, which also affects the optical information collection assembly to collect spectral information of the urine sample, and in order to improve the detection effect, the filtering process needs to be performed in this step.

S320, collecting the spectral information of the urine through the sample detection chamber.

The optical information acquisition assembly of the embodiment is arranged on one side of the sample detection chamber and can acquire spectral information from the sample detection chamber. The optical information acquisition assembly can store the spectral information of the acquired urine sample and hand over the spectral information to a detection position for detection, so that the detection accuracy is higher. In addition, in order to avoid detection errors, the spectral information can be called to perform secondary verification.

Before step S320, the present invention further includes:

and S321, filtering the light transmitted through the sample detection chamber.

In the foregoing step S311, although the interference of the background light source itself is eliminated, the interference of the external ambient light on the urine detection cannot be eliminated, so that the ambient light on the spectral information transmission path is filtered before the spectral information is collected, and accordingly, the collected spectral information is more accurate, and the finally obtained urine detection result is more accurate.

S330, urine detection is carried out according to the collected spectral information of the urine.

Specifically, the collected spectral information has different intensities, and the use of all spectral information for urine detection results in inaccurate detection results, so that some spectral information with proper intensity needs to be selected for detection, and the following steps are used for selecting the spectral information with proper intensity. It can be understood that, in the urine component detection method based on the fluorescent reagent, the intensity of the fluorescence used for acquiring the fluorescence image is also large or small, and the fluorescence with different intensities also causes inaccurate detection. Meanwhile, the urine detection method based on the microscopic image comprises the steps of controlling light of the background light source to penetrate through the cavity wall of the sample detection chamber to enter the sample detection chamber and collecting microscopic image information of urine through the sample detection chamber, wherein in the collection process of the microscopic image, the light intensity of the background light source is different, and erroneous judgment may be caused when the microscopic image of the urine is collected through the light source with different intensity. In summary, the urine detection method based on the microscopic image, the urine component detection method based on the fluorescent reagent, and the spectral detection method of the urine component have the wonderful effect of different and same works, in order to further improve the accuracy of the urine detection, the detection modes of the three can be recombined, and the recombined urine detection method is within the protection scope of the invention.

The S330 specifically includes the following steps:

s331, acquiring wavelength values of spectrum information, and arranging the spectrum information with different wavelengths into a first sequence according to a first preset mode, wherein the first sequence comprises a first noise area and a first characteristic peak area;

the first preset mode comprises a mode of increasing wavelength or decreasing wavelength, the noise region and the characteristic peak region are both regions with higher spectral intensity in the spectral information sequence, and the maximum spectral intensity of the characteristic peak region is greater than that of the noise region.

S332, obtaining intensity values of the spectrum information, and arranging the spectrum information of the first sequence into a second sequence according to a second preset mode;

the second predetermined manner includes a manner of increasing intensity or decreasing intensity.

S333, performing smooth filtering processing on the spectrum information of the second sequence;

s334, sequencing the spectrum information of the second sequence after the smoothing filtering treatment according to a third preset mode and defining the sequence as a third sequence, wherein the third sequence comprises a second noise region and a second characteristic peak region;

the third preset mode comprises a mode of increasing wavelength or decreasing wavelength, and the spectral information suitable for urine detection is positioned in the second characteristic peak area;

s335, acquiring a first amount of target spectrum information corresponding to the second characteristic peak area of the third sequence;

and S336, urine detection is carried out according to the first amount of target spectrum information.

The spectral information suitable for urine detection is located in the characteristic peak region, however, due to the existence of the noise region, the identification of the characteristic peak region is interfered, so that the characteristic peak region can be highlighted in the spectral information sequence only by preprocessing the spectrum and performing smooth filtering processing on the spectrum, and the essence of performing smooth filtering processing on the spectrum improves the signal fidelity of the spectral information in the characteristic peak region and the signal-to-noise ratio of the noise region.

The step S333 specifically includes:

s3331, continuously acquiring a second preset amount of spectrum information in a second sequence according to the intensity decreasing mode;

s3323, calculating a spectrum noise level according to a second preset amount of spectrum information, which mainly includes:

calculating an average intensity value and a standard deviation intensity value of a second preset amount of spectral information,

calculating a spectral noise level from the mean intensity value and the standard deviation intensity value;

s3324, calculating the width of a filtering window according to the spectrum noise level;

and S3325, performing smooth filtering processing according to the width of the filtering window.

Specifically, in the second sequence, the spectrum information with the intensity of t% at the top is selected and recorded as the noise sequence N, the amount of the spectrum information of t% at the top is the spectrum information of the second preset amount, then the spectrum intensity of each spectrum information in the second preset amount is obtained, and then the spectrum intensity of each spectrum information in the second preset amount is calculatedAverage value N of spectral intensity with spectral information_meanAnd standard deviation N_stdThe spectral noise level calculation method and the filtering window width calculation method are the prior art, and are not described herein again.

Finally, a Savitzky-Golay filter is selected for smoothing filtering, and the filtering method is the prior art and is not described herein. It is to be understood that the filtering processing manner is not limited thereto as long as smooth filtering can be achieved.

Preferably, step S336 includes:

in this embodiment, the first amount of target spectrum information selected is a certain amount of spectrum information acquired in the characteristic peak region, however, the spectrum information in the characteristic peak region does not necessarily satisfy the requirement, and therefore, abnormal information may also exist in the first amount of target spectrum information, and this step is to select the abnormal information, so as to make the urine detection result more accurate.

The ranking detection is suitable for the situation with the determined number of output data points, and aims to screen out a certain amount of spectral information with the intensity within the extreme value range from the first amount of target spectral information and remove the spectral information without being used for urine detection; the fuzzy detection is suitable for the situation that the number of output data points is not determined, and aims to screen out some spectral information which is worth paying attention to and remove the spectral information according to needs; in the ranking detection, the spectrum information between the maximum value and the minimum value is not processed, and the spectrum information between the maximum value and the minimum value is still possible to be abnormal, so that the spectrum information which is worth focusing between extreme values can be screened out by sequentially carrying out fuzzy detection on the basis of the ranking detection, and can be removed as required, and the urine detection precision can be further improved.

Wherein the ranking detection comprises:

carrying out urine detection according to the third preset amount of spectral information and the fourth preset amount of spectral information;

preferably, the third preset number is equal to the fourth preset number, and the maximum value and the minimum value are distributed uniformly, so that the detection precision is improved.

The blur detection includes:

sequencing the first amount of target spectrum information according to a fourth preset mode and defining the first amount of target spectrum information as a third sequence, wherein the fourth preset mode comprises a mode of increasing intensity or decreasing intensity;

determining P intensity sudden increase abnormal ranges with different intensities and Q intensity sudden decrease abnormal ranges with different intensities in the third sequence according to a fifth preset mode, where P, Q are positive integers, and the fifth preset mode may be an n-sigma detection mode, where the n-sigma detection mode is the prior art and is not described herein;

Preferably, in this embodiment, P is 3, Q is 3, μ is the average spectral intensity of the third sequence, and σ is the standard deviation of the spectral intensity of the third sequence, and anomaly detection is performed by using A3-sigma detection method, then, when screening the spectral information worth paying attention in the third sequence, the abrupt increase anomaly point range is considered to have three grades, a1, a2, and A3, the abrupt increase anomaly point a1> μ + σ, a2> μ +2 σ, and A3> μ +3 σ, where the spectral intensity related to the grade a1 is the highest, and the number of spectral information to be paid attention is based on the principle that the intensity of the spectral information is inversely proportional to the number of spectral information obtained according to intensity: a1 < A2 < A3; the sudden increase abnormal point range is considered to have three grades, B1, B2 and B3, then the sudden increase abnormal point B1 is less than mu-sigma, B2 is less than mu-2 sigma and B3 is less than mu-3 sigma, wherein, the spectrum intensity related to the grade B3 is the lowest, and the spectrum information quantity needing attention is determined according to the principle that the intensity of the spectrum information is in proportion to the spectrum information quantity obtained according to the intensity: b1 > B2 > B3.

After the quantity of the spectral information needing to be paid attention is determined, judging to reserve or remove the determined spectral information according to preset conditions, specifically, when the spectral information in a certain intensity range is set as the spectral information for urine detection, if the spectral information needing to be paid attention is in the range, summarizing the spectral information into the spectral information for urine detection; if the spectral information of interest lies outside this range, it is removed; if the intensity is on the boundary line of the intensity range, the retention or the removal is carried out according to the actual situation. Accordingly, urine detection accuracy can be improved.

Example 19

Referring to fig. 47 to 49, the present invention provides an electrochemical urine detection method for electrochemically detecting urine, where the conventional detection method is usually test paper detection, and after urine is dropped on the test paper, the test paper and urine send a chemical reaction, and the color change of the chemical reaction between the test paper and urine is observed and analyzed, so as to obtain a urine detection result, but comparing the reagent display result with a standard database by naked eyes may cause an inaccurate detection result, and therefore, the present invention electrochemically detects urine, including:

s400, detecting whether the electrochemical detection chip works normally or not;

only when the electrochemical detection chip works normally, the urine sample is dripped on the reaction part of the electrochemical detection chip for urine detection, so that the problem that the detection purpose cannot be achieved because the sample is dripped when the electrochemical detection chip works abnormally is avoided.

The electrochemical detection chip comprises an insulating substrate and a plurality of chip electrodes, wherein the chip electrodes are arranged on the insulating substrate to form a reaction part and a conductive part, urine is made to be a plurality in the reaction part, the chip electrodes are conducted to generate a plurality of electric signals to be transmitted to the conductive part for detection, the urine electrochemical indexes are detected through the electric signals, the relevant conditions of the urine are not judged through vision manually, and the detected urine data are more accurate.

Before step S400, the present invention further includes:

s401, adding a detection material on a chip electrode of the reaction part;

in the electrochemical detection of urine, it is necessary to add the same detection material or a plurality of detection materials to the chip electrode of the reaction portion.

When the same detection material is added on the chip electrode, a plurality of current values of a certain electrochemical index can be detected, and more accurate electrochemical index data can be obtained by calculating the average value of the plurality of current values.

When different detection materials are added on the chip electrode, a plurality of current values can be obtained to detect a plurality of electrochemical indexes of urine.

The electrochemical indexes comprise one or more of urine specific gravity, urine PH value, urine protein, uric acid, urine potassium, urine sodium, urine calcium, urine phosphorus, urine sugar and urine chloride, and can be obtained by electric signal detection and analysis. The chip electrode is provided with detection materials, and different detection materials can detect the different indexes, and the detection principle is the prior art and is not described herein again. The urine specific gravity and the urine pH value can be directly detected by the chip electrode, and detection materials do not need to be arranged.

S410, when the electrochemical detection chip works normally, dripping a urine sample on a reaction part of the electrochemical detection chip;

step S410 includes:

s411, sampling the urine to obtain a urine sample.

And when the electrochemical detection chip works abnormally, alarming information is sent out.

The abnormal state includes three kinds: the chip itself is damaged, the chip used last time is not pulled out, or the mounting position of the chip is inaccurate.

After the alarm information is sent out, the troubleshooting operation is required to be carried out, which comprises the following steps:

when the fault is that the chip is damaged, the chip is replaced and reinserted;

when the failure is that the chip used last time is not pulled out, the chip is replaced or inserted again;

and when the fault is that the mounting position of the chip is inaccurate, adjusting the mounting position of the chip.

After the troubleshooting operation is finished, the chip works normally and urine detection is continued.

S420, conducting identification is carried out on the electrochemical detection chip on which the urine sample is dripped, and the conducting identification is carried out by judging whether current is generated on the electrochemical detection chip or not;

the urine sample carries out the chemical reaction in reaction portion, and the chemical reaction can produce different current values, can assist through the detection current value and judge urine relevant data.

S430, when the electrochemical detection chip is identified to generate current through conduction, acquiring at least one current value on the electrochemical detection chip;

one current value can detect one electrochemical index, and when a plurality of electrochemical indexes need to be detected, a plurality of current values need to be acquired. Meanwhile, when a certain electrochemical index needs to be detected more accurately, a plurality of current values related to the certain electrochemical index need to be acquired, and then the average value of the plurality of current values is taken to obtain a more accurate current value.

S440, when the electric conduction identifies that no current is generated on the electrochemical detection chip, continuously dripping the urine sample on the reaction part of the electrochemical detection chip.

When the electrochemistry detects the chip normal operating, do not produce electric current after instiling into the urine and do not produce the urine sample and instil into the reaction portion or instil into the volume of the urine sample of reaction portion not enough, continue to instil into the urine sample and can know which kind of trouble belongs to.

Continuously dropping a urine sample, recognizing that current is generated on the electrochemical detection chip through conduction, removing the fault, and continuously detecting the urine;

and continuously dropping the urine sample, and recognizing that no current is generated on the electrochemical detection chip through conduction, so that whether the urine sample enters the electrochemical detection chip or not needs to be checked.

S450, comparing all current values with preset reference current values, and outputting detection results.

And when the detection result is output, prompting to remove the electrochemical detection chip. The electrochemical detection chip is stored for the next use.

After the detection result is output, the cleaning operation is started. And introducing cleaning liquid to clean the electrochemical urine detection device. The cleaning operation may be performed before the overflow of the electrochemical detection chip is prompted, and the electrochemical detection chip may be simultaneously cleaned.

According to the invention, the relevant conditions of the urine are judged without manual work through vision, and the urine data detected in a digital mode is more accurate.

The step S450 includes:

s451, setting a reference current sequence a, where a is a1, a2 … An, where n represents a sequence length and is a positive integer;

the reference current sequence a is a time sequence and changes with time, and as an undetected current sequence, in short, the reference current sequence a is a current sequence recorded before urine is not dropped, and recorded is a current on an electrochemical detection chip, and the reference current sequence a is used for removing noise, so as to eliminate the situation that the finally detected result is not ideal because environmental factors also change due to the change of time.

S452, setting a time current sequence B consisting of current values when current is generated on the electrochemical detection chip, wherein the sequence B is B1, B2 … Bm, m represents the sequence length and is a positive integer;

s453, screening a standard current sequence C from a current sequence template library according to the reference current sequence A and the m value, wherein the sequence C is C1 and C2 … Cm;

the time current sequence B is the current sequence recorded after the urine is dropped, and the current on the electrochemical detection chip is recorded.

The standard current sequence C is standard data of urine in a certain time, and is due data when a urine index is in an optimal condition, and is obtained through experiments, the data is unrelated to data detected according to current on an electrochemical detection chip, but in order to detect the data of the current urine index, the data needs to be compared with the data detected according to the current on the electrochemical detection chip, in this process, interference of environmental factors such as weather needs to be eliminated, the time current sequence B needs to be in the same external environment when compared with the standard current sequence C, and accordingly, values need to be taken according to the reference current sequence a and the m value.

S454, acquiring a first dynamic time warping distance DTW1 according to An and Cm;

s455, acquiring a second dynamic time warping distance DTW2 according to An and Bm;

comparing the first dynamic time warping distance DTW1 with the second dynamic time warping distance DTW2 can eliminate the interference of environmental factors such as weather on the electrochemical detection of urine.

The second dynamic time warping distance DTW2 between the reference current sequence a and the time current sequence B is calculated as follows:

a reference current sequence a ═ a1, An 2.. An, where n denotes the sequence length and is a positive integer;

time-current sequence B ═ B1, B2.. Bm, m denotes the sequence length and is a positive integer;

constructing a matrix of (n, m) and recording two points (a) in the (i, j) th cell_i，b_j) The Euclidean distance between d (a)_i，b_j)＝|a_i-b_j|。

As shown in fig. 49, a meandering path W, which is composed of several matrix elements connected to each other, describes a mapping between a and B. Let the kth cell be defined as w_k＝(i，j)_kThen, then

w＝w₁，w₂，w₃，..._，w_K，max(n，m)＜＝K＜＝n+m-1

This bent path satisfies the following condition:

1. boundary conditions are as follows: w is a₁Is ═ 1, and w_k＝(n，m)

2. Continuity: let w_k＝(a，b)，w_k-1(a ', b') then a-a '< ═ 1, b-b' < ═ 1

3. Monotonicity: let w_k＝(a，b)，w_k-1(a ', b'), then a-a '> ═ 0, b-b' > ═ 0

Among the plurality of paths satisfying the above condition, the shortest one that costs least is:

then, the distance between the two time series is:

r(i，j)＝d(i，j)+minr(i-1，j-1)，r(i-1，j)，r(i，j-1)。

DTW2＝r(i，j)。

the first dynamic time warping distance DTW1 between the reference current sequence A and the standard current sequence C is calculated in the same way as DTW 1.

And S456, comparing the first dynamic time warping distance DTW1 with the second dynamic time warping distance DTW2, and determining the detection result of the electrochemical detection chip.

If it is

The electrochemical detection result is considered to be normal, otherwise, the electrochemical detection result is abnormal.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for spectroscopic detection of a constituent of urine, the method comprising:

injecting urine into the sample detection chamber;

controlling light of a background light source to enter the sample detection chamber through the wall of the sample detection chamber;

2. The method of spectral detection according to claim 1, said method comprising: adjusting the temperature of urine in the sample detection chamber after the urine is injected into the sample detection chamber.

3. The method of spectral detection according to claim 1, said method comprising: and before the light of the background light source penetrates through the cavity wall of the sample detection chamber, filtering the light of the background light source to obtain light within a preset wave band range.

4. The method of spectral detection according to claim 1, said method comprising: and filtering the light transmitted through the sample detection chamber before collecting the spectral information of the urine.

5. The method for spectroscopic detection of claim 1 wherein the method comprises, prior to controlling the light of the background light source to enter the sample detection chamber through the walls of the sample detection chamber: a spectroscopic detection reagent is injected into the sample detection chamber.

6. The method for spectral detection according to claim 5, wherein said spectral detection comprises: at least one of Fourier infrared spectroscopy detection, Raman spectroscopy detection, fluorescence spectroscopy detection, and ultraviolet spectroscopy detection.

7. The spectroscopic method of any one of claims 1 to 6 wherein said detecting urine based on said collected spectroscopic information of said urine comprises:

and detecting urine according to the first amount of target spectrum information.

8. The method according to claim 7, wherein said step of performing a smoothing filtering process on the second sequence of spectral information comprises:

calculating a filtering window width according to the spectral noise level;

9. The method for spectrum detection according to claim 8, wherein said calculating a spectral noise level from a second predetermined amount of spectral information comprises:

10. The method for spectroscopic detection of claim 7 wherein said detecting urine from said first amount of target spectroscopic information comprises:

wherein the ranking detection comprises:

a third predetermined amount of spectral information is obtained from a decreasing intensity and a fourth predetermined amount of spectral information is obtained from a decreasing intensity,

the blur detection comprises: