CN114525318A - Method for separating beet polysaccharide and betaine by using compound enzyme coupled continuous nanofiltration membrane - Google Patents
Method for separating beet polysaccharide and betaine by using compound enzyme coupled continuous nanofiltration membrane Download PDFInfo
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- CN114525318A CN114525318A CN202210073581.6A CN202210073581A CN114525318A CN 114525318 A CN114525318 A CN 114525318A CN 202210073581 A CN202210073581 A CN 202210073581A CN 114525318 A CN114525318 A CN 114525318A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/007—Carnitine; Butyrobetaine; Crotonobetaine
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
Abstract
The invention discloses a method for separating beet polysaccharide and betaine by a composite enzyme coupled continuous nanofiltration membrane, which relates to the technical field of separation of effective components and comprises the following steps: s1: adding a complex enzyme into the beet extract, and carrying out enzymolysis to obtain an enzymolysis extract, wherein the complex enzyme is a mixture of pectinase, amylase and xylanase; s2: clarifying the enzymolysis leachate with an ultrafiltration membrane, decoloring with active carbon, and performing primary nanofiltration to obtain trapped fluid 1 and permeate fluid 1; carrying out secondary nanofiltration on the permeate 1 to obtain trapped fluid 2 and permeate 2; the trapped fluid 1 is a beet polysaccharide concentrated solution, and the permeate liquid 2 is a betaine concentrated solution. The complex enzyme coupling continuous nanofiltration membrane process not only can continuously separate beet polysaccharide and betaine from beet exudation juice with high recovery rate, but also reduces membrane pressure, prolongs the service life of the membrane, reduces energy consumption, and is suitable for the durable sugar making process requirement.
Description
Technical Field
The invention relates to the technical field of effective component separation, in particular to a method for separating beet polysaccharide and betaine by a composite enzyme coupled continuous nanofiltration membrane.
Background
Beet is one of the main sugar crops, and beet sugar production accounts for about 25 percent of the global sugar production, and the production scale and the generated economic benefit are huge. In order to improve the purity of sugar products, most of domestic sugar factories adopt a carbonation cleaning process to clarify beet exudation juice in the sugar production process by a double carbonic acid method, but the process has high energy consumption and low sugar production efficiency, and some non-sugar components are still remained in concentrated sugar solution and are not effectively removed, thereby influencing the crystallization rate and the quality of the final sugar products. More non-sugar components become waste honey and then flow into underground water through discharge, which wastes resources and causes water pollution to a certain degree.
The non-sugar active ingredients include pigment substances, organic macromolecular substances (polysaccharides and pectins) and small molecule substances (betaines and reducing sugars). The beet polysaccharide is an active polysaccharide with a molecular weight range of 0.74-300 kDa, and has important physiological activities of oxidation resistance, bacteriostasis, immune function regulation and the like. Betaine is a quaternary ammonium type alkaloid, has a molecular weight of 117.15, has activities of resisting oxidation, improving cardiovascular diseases, improving intestinal functions, resisting tumors and the like, and is widely applied to the fields of clinical treatment, foods, cosmetics and the like.
Therefore, the problem to be solved by those skilled in the art is to provide a beet sugar manufacturing process for comprehensively developing beet polysaccharide and betaine.
Disclosure of Invention
In view of the above, the invention provides a method for separating beet polysaccharide and betaine by a complex enzyme coupled continuous nanofiltration membrane.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for separating beet polysaccharide and betaine by a composite enzyme coupled continuous nanofiltration membrane comprises the following steps:
s1: adding a complex enzyme into the beet extract, and carrying out enzymolysis to obtain an enzymolysis extract, wherein the complex enzyme is a mixture of pectinase, amylase and xylanase;
s2: clarifying the enzymolysis leachate with an ultrafiltration membrane, decoloring with active carbon, and performing primary nanofiltration to obtain trapped fluid 1 and permeate fluid 1;
carrying out secondary nanofiltration on the permeate 1 to obtain trapped fluid 2 and permeate 2;
the trapped fluid 1 is a beet polysaccharide concentrated solution, and the permeate liquid 2 is a betaine concentrated solution. The trapped fluid 2 is a sucrose concentrated solution.
Has the advantages that: the method adopts low-temperature complex enzyme enzymolysis coupled with multi-stage nanofiltration membrane filtration, and improves the yield of the beet polysaccharide and the betaine and the conversion rate of raw materials by reducing the content of suspended matters in the feed liquid and the content of water-insoluble pectin which is not beneficial to membrane separation.
As the preferable technical scheme of the invention, the compound enzyme is low-temperature compound enzyme, the addition amount of the compound enzyme is 3 per mill of the beet leaching solution in weight volume ratio,
and the weight ratio of pectinase, amylase and xylanase in the compound enzyme is 7:2: 1.
More preferably, the enzyme activity of the pectinase is 500U/mg, the enzyme activity of the amylase is 50U/mg, and the enzyme activity of the xylanase is 6000U/mg.
As a preferable technical scheme of the invention, the temperature of the enzymolysis of S1 is 45 ℃, the time is 30 min-2 h, and the pH value of the enzymolysis is initially adjusted to 5.
More preferably, the enzymolysis time is 2 h.
More preferably, the pH is adjusted with citric acid.
As a preferable technical scheme of the invention, the membrane cut-off molecular weight of the first-stage nanofiltration of S2 is 200-800 Da, and the membrane cut-off molecular weight of the second-stage nanofiltration is 150-200 Da.
The primary nanofiltration membrane permeates most of the beet crude polysaccharide, and the betaine, the disaccharide (containing sucrose) and the monosaccharide (containing glucose) are permeated; the permeated components are separated by a secondary nanofiltration membrane, and the sucrose is intercepted to permeate the betaine and the monosaccharide. The effective separation of the beet polysaccharide, the sucrose and the betaine is realized through two-stage nanofiltration.
More preferably, the pressure of the primary nanofiltration device and the secondary nanofiltration device is 1.0-8.0 MPa, the pH value is 9.0-10.0, the temperature is 35-55 ℃, and the time is 60 min.
More preferably, the pressure of the device for the first-stage nanofiltration and the second-stage nanofiltration is 3.0MPa, and the temperature is 55 ℃.
In a preferred embodiment of the present invention, the beet leach liquor of S1 is obtained by washing and shredding beet, extracting with water, draining in a drainer, and removing impurities in a hydrocyclone.
As a preferred technical scheme of the invention, the aperture of the ultrafiltration membrane of S2 is 15kDa, the enzymolysis leaching solution is kept stand for more than 30min, and the supernatant is taken to pass through the ultrafiltration membrane.
As a preferable technical scheme of the invention, the activated carbon decolorization of S2 controls the pH value to be 9-10, the temperature to be 50 ℃ and the time to be 15 min.
As a preferable technical scheme of the invention, the method also comprises the following post-treatment steps: s2, concentrating the beet polysaccharide concentrated solution, and freeze-drying to obtain beet polysaccharide; concentrating the obtained betaine concentrated solution, and freeze drying to obtain betaine; the obtained sucrose concentrate is immersed in a sugar preparation process to prepare sucrose.
More preferably, the concentration temperature of the beet polysaccharide is 75-80 ℃, the concentration vacuum degree is 0.1MPa, the freeze drying vacuum degree is 0.1KPa, the temperature is-40 ℃, and the time is 48 h; the concentration temperature of betaine is 70 deg.C, concentration vacuum degree is 0.1MPa, freeze drying vacuum degree is 0.1KPa, temperature is-40 deg.C, and time is 48 h.
According to the technical scheme, compared with the prior art, the method (1) adopts a low-temperature enzymolysis process, so that beet polysaccharide is greatly reserved, and the polysaccharide decomposition caused by high temperature is avoided; (2) the pectin in the leaching solution is subjected to enzymolysis, so that the viscosity of the feed liquid is greatly reduced, the pressure of membrane operation is reduced, and the required energy consumption is reduced; (3) the activated carbon is used for adsorbing the pigment, so that the purity of the beet polysaccharide and the betaine is increased. The method is mainly characterized by high efficiency, environmental protection and improvement of raw material conversion rate, is a method for continuously recovering effective active ingredients with different molecular weights from the beet extract, can meet the demand of the sugar production process, can fully utilize resources and simultaneously reduce environmental pollution.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a flow chart of a separation and concentration process in example 1 of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The raw materials and equipment which are not described in detail in the examples are all commercially available conventional production testing equipment, and are not described herein again.
Example 1
A method for separating beet polysaccharide and betaine by using a composite enzyme coupled continuous nanofiltration membrane comprises the following steps (see figure 1):
(1) pretreatment: cleaning beet, shredding, continuously percolating with water in an exudator, and removing impurities in a cyclone to obtain beet leachate.
(2) Compound enzymolysis: adding 3 ‰ (w/v) complex enzyme (pectase: amylase: xylanase: 7:2:1) into beet lixivium, mixing, adjusting pH to 5 with citric acid, and heating to 45 deg.C for enzymolysis for 2 hr to obtain enzymolysis lixivium. The treatment capacity of the enzymolysis tank is 300m in the embodiment3/h。
(3) Clarifying and decoloring: naturally settling the enzymolysis leachate for 30min, and clarifying the supernatant with an ultrafiltration membrane and decolorizing with active carbon; the model of the ultrafiltration membrane is a tubular ultrafiltration membrane, and the back pressure of the membrane is kept at 0.2 MPa.
(4) And (3) a continuous nanofiltration membrane process: the clarified and decolored feed liquid passes through a two-stage spiral nanofiltration membrane, the membrane front pressure is controlled to be 3-7 Mpa, and the feed liquid passes through a first-stage nanofiltration membrane to obtain trapped liquid 1 (beet polysaccharide concentrated solution) and permeate liquid 1; the permeate 1 enters a secondary nanofiltration to obtain a permeate 2 (betaine concentrated solution) and an retentate 2, and the retentate 2 is a sucrose concentrated solution and continues to enter a sugar making process.
(5) Concentrating and drying: and concentrating the trapped fluid 1 at 75-80 ℃ under the vacuum degree of 0.1MPa until the content of soluble solids in the feed liquid is 20-25 ℃, and freeze-drying at the vacuum degree of 0.1KPa and the temperature of-40 ℃ for 48 hours to obtain a dried beet polysaccharide product V.
And concentrating the permeate 2 at 70 ℃ and 0.1MPa until the content of soluble solids in the feed liquid is 20-25 ℃, and freeze-drying at-40 ℃ for 48 hours at 0.1KPa in vacuum to obtain a betaine crude dry product V.
In the enzymolysis process, the suspended matter sedimentation time and the suspended matter amount are respectively tested by sampling for 15min, 30min, 60min, 90min, 120min and 150 min. The amount of sediment is the volume of suspended matter at the bottom after full sedimentation. After sufficient sedimentation, the weight ratio of the supernatant to the sediment was measured and found to be 1.5ml/50ml (pH 5.0), 3ml/50ml (pH 4.9), 4ml/50ml (pH 4.8), 5ml/50ml (pH 4.6), 9ml/50ml (pH 4.4) and 10ml/50ml (pH 4.4), respectively. Therefore, the sedimentation effect is obvious in 120min, and with the prolonging of the enzymolysis time, the easily-sedimented solid which is wrapped by the pectin is separated out after the pectin is decomposed, and the sedimentation performance of the beet extract after the enzymolysis is greatly improved due to the flocculation effect.
Comparative example 1
Examine the effect of different enzyme treatments without continuous nanofiltration membranes
(1) Pretreatment: cleaning beet, shredding, percolating with water in a percolating device, and removing impurities in a cyclone to obtain beet leachate.
(2) Enzymolysis: adding 3 ‰ (w/v) enzyme into beet leachate, mixing, adjusting pH to 5 with citric acid, heating to 45 deg.C, and performing enzymolysis for 2 hr to obtain enzymolysis leachate. The treatment capacity of the enzymolysis tank is 300m in the embodiment3/h。
The viscosity change of the feed solution after different enzyme treatments is shown in table 1.
TABLE 1
Note: the complex enzyme I is pectinase: cellulase is 8:2, complex enzyme II is pectinase: amylase: xylanase 7:2: 1. The activities of the enzymes used in the examples of the invention and the comparative examples were: 500U/mg of pectinase, 50U/mg of cellulase, 50U/mg of amylase and 6000U/mg of xylanase.
(3) Clarifying and decoloring: because the enzymolysis in the previous step has the best effect by using the compound enzyme I and the compound enzyme II, the enzymolysis leachate of the compound enzyme I and the compound enzyme II is respectively collected, kept stand and settled for 30min, and the supernatant is respectively clarified by an ultrafiltration membrane and decolorized by active carbon; the model of the ultrafiltration membrane is a tubular ultrafiltration membrane, and the back pressure of the membrane is kept at 0.2 MPa.
(4) Concentrating and drying: concentrating and drying the decolorized feed liquid of the complex enzyme I at 80 ℃ and the vacuum degree of 0.1MPa until the content of soluble solids in the feed liquid is 20-25 ℃, and freeze-drying for 48 hours at the temperature of-40 ℃ under the vacuum degree of 0.1KPa to obtain a crude dried product I of the mixture of the beet polysaccharide and the betaine.
Concentrating and drying the decolorized feed liquid of the compound enzyme II at 80 ℃ and the vacuum degree of 0.1MPa until the content of soluble solids in the feed liquid is 20-25 ℃, and freeze-drying for 48 hours at the temperature of-40 ℃ under the vacuum degree of 0.1KPa to obtain a crude dried product II of the mixture of the beet polysaccharide and the betaine.
Comparative example 2
Investigating the Effect without enzymatic hydrolysis
(1) Pretreatment: cleaning beet, shredding, percolating with water in a percolating device, and removing impurities in a cyclone to obtain beet leachate.
(2) Clarifying and decoloring: clarifying the beet leachate by an ultrafiltration membrane and decoloring by active carbon; the model of the ultrafiltration membrane is a tubular ultrafiltration membrane, and the back pressure of the membrane is kept at 0.6 MPa.
(3) And (3) a continuous nanofiltration membrane process: the clarified and decolored feed liquid is subjected to a two-stage roll-type ultrafiltration membrane, the membrane front pressure is controlled to be 5-9 Mpa, and the feed liquid is subjected to one-stage nanofiltration to obtain trapped liquid 1 (beet polysaccharide concentrated solution) and permeate liquid 1; the permeate 1 enters a secondary nanofiltration to obtain a permeate 2 (betaine concentrated solution) and an retentate 2, and the retentate 2 is a sucrose concentrated solution and continues to enter a sugar making process.
(5) Concentrating and drying: and concentrating the trapped fluid 1 at 75-80 ℃ and 0.1MPa until the content of soluble solids in the feed liquid is 20-25 ℃, and freeze-drying at-40 ℃ for 48h under 0.1KPa of vacuum degree to obtain a dried beet polysaccharide product III.
And concentrating the permeate 2 at 70 ℃ and 0.1MPa until the content of soluble solids in the feed liquid is 20-25 ℃, and freeze-drying at-40 ℃ for 48h under the vacuum degree of 0.1KPa to obtain a betaine crude dry product III.
Comparative example 3
Investigating the effect of different kinds of complex enzyme and multi-stage nanofiltration
(1) Pretreatment: cleaning and shredding beet, leaching with water as extractive solution in a leaching device, and removing impurities in a cyclone to obtain beet leachate.
(2) Compound enzymolysis: adding 3 ‰ (w/v) complex enzyme (pectinase: cellulase: 8:2) into beet lixivium, mixing, adjusting pH to 5 with citric acid, and heating to 45 deg.C for enzymolysis for 2 hr to obtain enzymolysis lixivium. The treatment capacity of the enzymolysis tank is 300m in the embodiment3/h。
(3) Clarification and decoloration: standing and settling the enzymolysis leaching solution for 30min, and clarifying the supernatant through an ultrafiltration membrane and decoloring the supernatant through active carbon; the model of the ultrafiltration membrane is a tubular ultrafiltration membrane, and the back pressure of the membrane is kept at 0.2 MPa.
(4) And (3) a continuous nanofiltration membrane process: the clarified and decolored feed liquid passes through a two-stage roll-type ultrafiltration membrane, the pressure in front of the membrane is controlled to be 3-7 Mpa, and the feed liquid is subjected to primary nanofiltration to obtain trapped liquid 1 (beet polysaccharide concentrated solution) and permeate liquid 1; the permeate 1 enters a secondary nanofiltration to obtain a permeate 2 (betaine concentrated solution) and an retentate 2, and the retentate 2 is a sucrose concentrated solution and continues to enter a sugar making process.
(5) Concentrating and drying: and concentrating the trapped fluid 1 at 75-80 ℃ under the vacuum degree of 0.1MPa until the content of soluble solids in the feed liquid is 20-25 ℃, and freeze-drying at the vacuum degree of 0.1KPa and the temperature of-40 ℃ for 48 hours to obtain a dried beet polysaccharide product IV.
And concentrating the permeate 2 at 70 ℃ and 0.1MPa until the content of soluble solids in the feed liquid is 20-25 ℃, and freeze-drying at-40 ℃ for 48 hours under the vacuum degree of 0.1KPa to obtain a betaine crude dry product IV.
Example 2
The dried products of betaine polysaccharides and betaine or mixed crude products of betaine polysaccharides and betaine obtained in example 1 and comparative examples 1-3 were tested for their polysaccharide and betaine contents, and the results are shown in Table 2.
Measuring the yield of the crude polysaccharide by a phenol-sulfuric acid method (the mass g of the crude polysaccharide is g/the weight g of the raw material is multiplied by 100 percent), and measuring the purity of the betaine in the freeze-dried crude product by an HPLC method (the mass g of the betaine is g/the weight of the crude product is multiplied by 100 percent); betaine yield (betaine mass g/raw material weight g × 100%), yield (total betaine polysaccharide weight g/raw material weight g × 100%).
TABLE 2
The results show that after the complex enzymes I and II are treated, monosaccharide, disaccharide and pectin enzymolysis products (galacturonic acid) in the exudate enter an ultrafiltration stage together with polysaccharide substances and are filtered out together as filtrate, the effect of separating polysaccharide and betaine cannot be achieved, and a large amount of monosaccharide, various small molecular pigments, ions and part of other soluble organic matters are mixed together, so that the measured result of the phenol-sulfuric acid method is higher, and the purity of the betaine is lower. When only nanofiltration is carried out, a large amount of pectin in the ultrafiltration stage is partially dissolved in water and partially exists in a suspended state and wraps a large amount of easily-settled solids, so that a large amount of pectin is intercepted by the ultrafiltration membrane, the flux of the ultrafiltration membrane is low, the efficiency is low, and finally the polysaccharide interception rate of a nanofiltration section is low and the impurity content is high. The complex enzyme coupling continuous nanofiltration membrane process not only can continuously separate beet polysaccharide and betaine from beet exudation juice with high recovery rate, but also reduces membrane pressure, prolongs the service life of the membrane, reduces energy consumption, and is suitable for the durable sugar making process requirement.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A method for separating beet polysaccharide and betaine by a composite enzyme coupled continuous nanofiltration membrane is characterized by comprising the following steps:
s1: adding a complex enzyme into the beet extract, and carrying out enzymolysis to obtain an enzymolysis extract, wherein the complex enzyme is a mixture of pectinase, amylase and xylanase;
s2: clarifying the enzymolysis leachate with an ultrafiltration membrane, decoloring with active carbon, and performing primary nanofiltration to obtain trapped fluid 1 and permeate fluid 1;
carrying out secondary nanofiltration on the permeate 1 to obtain trapped fluid 2 and permeate 2;
the trapped fluid 1 is a beet polysaccharide concentrated solution, and the permeate liquid 2 is a betaine concentrated solution.
2. The method as claimed in claim 1, wherein the complex enzyme S1 is added in an amount of 3 ‰ of beet leachate in weight volume ratio,
and the weight ratio of pectinase, amylase and xylanase in the compound enzyme is 7:2: 1.
3. The method according to claim 1, wherein the temperature of the enzymolysis in S1 is 45 ℃, the time is 30 min-2 h, and the pH value of the enzymolysis is initially adjusted to 5.
4. The method of claim 1, wherein the membrane cut-off molecular weight of the first-stage nanofiltration of S2 is 200-800 Da, and the membrane cut-off molecular weight of the second-stage nanofiltration is 150-200 Da.
5. The method according to claim 4, wherein the pressure of the primary nanofiltration and the secondary nanofiltration device is 1.0-8.0 MPa, the pH is 9.0-10.0, the temperature is 35-55 ℃, and the time is 60 min.
6. The process according to claim 5, characterized in that the pressure of the device for the primary and secondary nanofiltration is 3.0MPa and the temperature is 55 ℃.
7. The method according to claim 1, wherein the beet leach liquor of S1 is obtained by washing and shredding beet, extracting with water, leaching, and removing impurities.
8. The method of claim 1, wherein the ultrafiltration membrane of S2 has a pore size of 15 kDa.
9. The method according to claim 1, wherein the activated carbon decolorization of S2 is performed by controlling the pH value at 9-10, the temperature at 50 ℃ and the time at 15 min.
10. The method of claim 1, further comprising a post-processing step of: s2, concentrating the beet polysaccharide concentrated solution, and freeze-drying to obtain beet polysaccharide; concentrating the obtained betaine concentrated solution, and freeze drying to obtain betaine; the obtained sucrose concentrate is immersed in a sugar preparation process to prepare sucrose.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040006222A1 (en) * | 2002-06-26 | 2004-01-08 | Finnfeeds Finland Oy | Process for recovering betaine |
| CN105566137A (en) * | 2015-12-25 | 2016-05-11 | 安徽丰原发酵技术工程研究有限公司 | Method for separation and purification of betaine from beet molasses |
| CN105861761A (en) * | 2016-03-31 | 2016-08-17 | 刘伟亚 | Method for preparing black sugar |
| CN109355440A (en) * | 2018-11-16 | 2019-02-19 | 成都连接流体分离科技有限公司 | A kind of system and technique of continuous film production beet sugar |
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2022
- 2022-01-21 CN CN202210073581.6A patent/CN114525318A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040006222A1 (en) * | 2002-06-26 | 2004-01-08 | Finnfeeds Finland Oy | Process for recovering betaine |
| CN105566137A (en) * | 2015-12-25 | 2016-05-11 | 安徽丰原发酵技术工程研究有限公司 | Method for separation and purification of betaine from beet molasses |
| CN105861761A (en) * | 2016-03-31 | 2016-08-17 | 刘伟亚 | Method for preparing black sugar |
| CN109355440A (en) * | 2018-11-16 | 2019-02-19 | 成都连接流体分离科技有限公司 | A kind of system and technique of continuous film production beet sugar |
Non-Patent Citations (3)
| Title |
|---|
| MIKAEL SJÖLIN 等: ""Purification of Sucrose in Sugar Beet Molasses by Utilizing Ceramic Nanofiltration and Ultrafiltration Membranes"", 《MEMBRANES》, vol. 10, pages 687 - 688 * |
| 张龙 等: "《绿色化学》", vol. 2, 华中科技大学出版社, pages: 276 * |
| 王宝 等: ""纳滤技术在甜菜制糖澄清工艺中的应用研究"", 《中国糖料》, vol. 40, no. 1, pages 1 - 4 * |
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