CN114522134A - Method for promoting skin barrier regeneration by over-expressing lncRNA H19 in mesenchymal stem cells - Google Patents
Method for promoting skin barrier regeneration by over-expressing lncRNA H19 in mesenchymal stem cells Download PDFInfo
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- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A61H—PHYSICAL THERAPY APPARATUS, e.g. DEVICES FOR LOCATING OR STIMULATING REFLEX POINTS IN THE BODY; ARTIFICIAL RESPIRATION; MASSAGE; BATHING DEVICES FOR SPECIAL THERAPEUTIC OR HYGIENIC PURPOSES OR SPECIFIC PARTS OF THE BODY
- A61H23/00—Percussion or vibration massage, e.g. using supersonic vibration; Suction-vibration massage; Massage with moving diaphragms
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- A61H7/00—Devices for suction-kneading massage; Devices for massaging the skin by rubbing or brushing not otherwise provided for
- A61H7/002—Devices for suction-kneading massage; Devices for massaging the skin by rubbing or brushing not otherwise provided for by rubbing or brushing
- A61H7/004—Devices for suction-kneading massage; Devices for massaging the skin by rubbing or brushing not otherwise provided for by rubbing or brushing power-driven, e.g. electrical
- A61H7/005—Devices for suction-kneading massage; Devices for massaging the skin by rubbing or brushing not otherwise provided for by rubbing or brushing power-driven, e.g. electrical hand-held
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Abstract
The invention provides a method for promoting skin barrier regeneration by over-expressing lncRNA H19 in mesenchymal stem cells, which is characterized by comprising a skin barrier repair solution, specifically comprising a mesenchymal stem cell-derived exosome lncRNA H19 suspension, glycerol, isoamyl acetate, phosphatidylinositol-4, 5-diphosphate 3-kinase and a hydrosolvent; the smearing device comprises a main body, a smearing main body and a cover body; the front end of the main body is provided with the smearing main body, the cover body is arranged outside the smearing main body, the power supply assembly and the control assembly are arranged inside the main body, the liquid pushing rod is controlled to extrude the repairing liquid in a reciprocating mode, the repairing liquid flows out of the smearing main body to be smeared, and meanwhile the massaging function is achieved. The invention provides a new repair mode for skin barrier repair, develops cell repair liquid, can be used for skin repair and has wide application prospect; the massage head is convenient to carry and store in the whole structure, the use mode is simple, the massage head is combined with the smearing area, absorption of the repairing liquid is promoted, and a better effect can be achieved.
Description
Technical Field
The invention relates to the field of application of cell exosomes, in particular to a method for promoting skin barrier regeneration by over-expressing lncRNA H19 by mesenchymal stem cells, which is embodied as skin barrier repair liquid and a smearing device thereof.
Background
The skin barrier mainly comprises a physical barrier, and particularly comprises a sebum membrane, horny layer keratin, lipid, a sandwich structure, a brick wall structure, dermal mucopolysaccharide, mucopolysaccharide and the like, and has the effects of resisting external harmful substances, irritants and sunlight, and moisturizing, regulating and resisting inflammation. Skin physical barrier damage can cause skin dryness, skin aging, pigmentation atopic dermatitis, eczema, psoriasis, ichthyosis, skin sensitivity such as solar dermatitis, skin greasiness such as irritant dermatitis and hormone-dependent dermatitis, seborrheic diseases such as skin allergy, external oil dryness, skin care product absorption difficulty and a large amount of red blood streak on the face, and the like.
For a long time, many scholars and enterprises are dedicated to research and produce methods capable of helping skin barrier injury to be repaired, but the effectiveness is limited, and no efficient, harmless and low-cost skin barrier repair method exists.
Mesenchymal Stem Cells (MSCs) are a class of primitive undifferentiated Cells with multipotentiality and self-renewal capacity, with the ability to differentiate towards mesoderm, ectoderm and endoderm.
Disclosure of Invention
The invention aims to provide a method for promoting skin barrier regeneration by over-expressing lncRNA H19 in mesenchymal stem cells, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for promoting skin barrier regeneration by over-expressing lncRNA H19 in mesenchymal stem cells is characterized by comprising a skin barrier repair liquid and an application device of the skin barrier repair liquid, wherein the application device is used for applying the skin barrier repair liquid to the damaged part of the skin barrier while performing punch type massage application.
The skin barrier repair liquid is characterized by comprising a suspension of mesenchymal stem cell MSC-derived exosome IncRNA H19, glycerol, isoamyl acetate, phosphatidylinositol-4, 5-bisphosphate 3-kinase and a water solvent, and specifically comprises 20-30 parts of a suspension of mesenchymal stem cell MSC-derived exosome IncRNA H19, 20-30 parts of glycerol, 10-15 parts of isoamyl acetate, 5-10 parts of phosphatidylinositol-4, 5-bisphosphate 3-kinase and 45-50 parts of a water solvent; fully fusing exosome lncRNA H19 suspension derived from mesenchymal stem cells MSC with glycerol to ensure that the temperature is always 26-30 ℃, centrifuging for 5min by a centrifuge to obtain supernatant as a product A, taking subnatant as a product B, mixing the product B with phosphatidylinositol-4, 5-bisphosphate 3-kinase to obtain a product C, and fully mixing the product A and the product C with isoamyl acetate, glycerol and a hydrosolvent to obtain the skin barrier repair liquid.
The mesenchymal stem cells are applied to promoting skin barrier regeneration; PTEN was upregulated by MicroRNA-152-3p to promote wound healing on the skin surface.
The smearing device for the skin barrier repair liquid is characterized in that: comprises a main body 1, an applying main body 2 and a cover body 3; the front end of the main body 1 is provided with the smearing main body 2, the cover body 3 is arranged outside the smearing main body 2, a power supply assembly and a control assembly are arranged inside the main body 1, the repair liquid is controlled to be extruded back and forth by the liquid pushing rod 43, the repair liquid flows out of the smearing main body 2 and is smeared, and the smearing main body 2 is provided with the massage end 22 to assist the smearing main body 2 in implementing a massage function at the same time.
The main body 1 comprises a handle end 11 and an applying end 12, wherein the applying end 12 and the handle end 11 are integrated, and a cavity is formed inside the applying end 12; an external port 111 is arranged at the bottom of the handle end 11 and used for externally connecting a cosmetic instrument, and a switch button 121, a first reciprocating button 122 and a second reciprocating button 123 are arranged at the junction of the smearing end 12 and the handle end 11 and used for controlling the reciprocating motion of the liquid pushing rod 43; a liquid viewing window 124 is arranged at the middle lower part of the smearing end 12, is a transparent window and is used for observing the use state of the internal repairing liquid; the front end of the smearing end 12 is provided with a first concave ring 125.
The front end of the main body 1 and the tail end of the smearing main body 2 are provided with spiral openings, so that repair liquid can be added or replaced conveniently.
The smearing main body 2 comprises a liquid outlet plate 21, a massaging end 22 and a massaging brush 23, wherein the center of the liquid outlet plate 21 is provided with a plurality of liquid outlet holes 211 with different sizes, specifically, the outer ring of the liquid outlet holes are larger than the inner ring of the liquid outlet holes, and a plurality of liquid outlet holes 211 are arranged in each ring; the massage ends 22 are arranged on the periphery of the liquid outlet hole 211, the massage brush 23 is embedded or bonded at the bottom of the massage end 22, the massage ends 22 are three strip-shaped massage heads with radian, the periphery of the liquid outlet hole 211 plays a role in collecting repair liquid to prevent waste, and an auxiliary liquid channel 24 is arranged between every two massage ends 22 to prevent the unsatisfactory smearing effect caused by excessive accumulation of central repair liquid; the massage end 22 is provided with a plurality of massage control components, specifically, a massage base 221, a vibration area 222, a power control area 223 and a pressure sensing area 224, wherein the vibration area 222 is disposed below the power control area 223, the pressure sensing area 224 is disposed above the power control area 223, and the massage base 221 is disposed outside the vibration area 222, the power control area 223 and the pressure sensing area 224 as a housing.
A second concave ring 31 is arranged on the periphery of the inner part of the cover body 3, a magnetic stone is embedded in the second concave ring 31 and mutually attracted with the magnetic stone in a first concave ring 125 arranged at the front end of the smearing main body 2 to assist in closing the cover body 3; the magnet is the same as the first concave ring 125 and the second concave ring 31.
The power supply assembly and the control assembly are arranged inside the main body 1, the power supply assembly is mainly a power supply 41 and comprises an upper end fixing piece 411, a power supply main body 412 and a lower end fixing piece 412, the upper end fixing piece 411 is arranged above the power supply main body 412, the lower end fixing piece 412 is arranged below the power supply main body 412, the upper end fixing piece and the lower end fixing piece fix the power supply main body 412 inside the handle end 11 of the main body 1 through a fixing nut assembly 414, and a second connecting end 415 is arranged on one side of the power supply main body 412 and used for being connected with a first connecting end 421; the control assembly comprises a liquid pushing rod fixer 42, a liquid pushing rod 43 and a liquid pushing plate 44, wherein the upper end of the liquid pushing rod 43 is fixed inside the main body 1 through the liquid pushing rod fixer 42, the lower end of the liquid pushing rod 43 is directly connected with the liquid pushing plate 44, a control area is arranged inside the liquid pushing rod fixer 42, and the control area extends out of the control area and is used for being connected with the second connecting end 415.
A use method of an applying device of skin barrier repair liquid is characterized in that: the method comprises the following steps:
s1: adding a skin barrier repair liquid into the main body 1;
s2: the first reciprocating button 122 and the second reciprocating button 123 are controlled to discharge liquid, and the use condition of the liquid is observed through a liquid viewing window;
s3: the applied part is placed on the skin surface and is slightly applied with force, and the pressure sensing area 224 senses the pressure release signal and transmits the signal to the power control area 223, so as to control the vibration area 222 to vibrate, massage and promote the absorption of the repair liquid.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a creative method for repairing damaged skin barriers, provides a new repairing mode for repairing skin barriers, develops cell repairing liquid, can be used for repairing skins, and has wide application prospect. The invention also provides an application device specially used for applying the skin barrier modification liquid, the whole structure is convenient to carry and store, the use mode is simple, the massage head is combined with the application area, the absorption of the repair liquid is promoted, and a better effect can be achieved.
Drawings
FIG. 1 is a schematic view of the overall structure of an application device of the skin barrier repair liquid of the present invention;
FIG. 2 is a schematic view of the housing of the applying device for skin barrier repair liquid according to the present invention;
FIG. 3 is a schematic view of the main body structure of the device for applying the skin barrier repair liquid according to the present invention;
FIG. 4 is a schematic view of a massage end of the application device of the skin barrier repair liquid of the present invention;
FIG. 5 is a schematic view of one of the power supply and control components of the device for applying a skin barrier repair liquid according to the present invention;
FIG. 6 is a second schematic view of the power supply and control components of the device for applying a skin barrier repair liquid according to the present invention;
in the figure: 1. a main body; 11. a handle end; 111. an external port; 12. coating end; 121. a switch button; 122. a first shuttle button; 123. a second shuttle button; 124. a sight window; 125. a first concave ring; 2. smearing the main body; 21. a liquid outlet plate; 211. a liquid outlet hole; 22. a massage end; 221. a massage base; 222. a vibration area; 223. a power supply control area; 224. a pressure sensing area; 23. a massage brush; 24. an auxiliary fluid channel; 3. a cover body; 31. a second concave ring; 41. a power source; 411. an upper end fixing member; 412. a power supply main body; 413. a lower end fixing member; 414. a fixing nut assembly; 415. a second connecting end; 42. a push rod holder; 421. a first connection end; 43 a liquid pushing rod; 44. a liquid pushing plate.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is to be understood that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work based on the embodiments of the present invention belong to the scope of the present invention.
A method for promoting skin barrier regeneration by over-expressing lncRNA H19 in mesenchymal stem cells is characterized by comprising a skin barrier repair liquid and an application device of the skin barrier repair liquid, wherein the application device is used for applying the skin barrier repair liquid to the damaged part of the skin barrier while performing punch type massage application.
Example 1
The skin barrier repair liquid is characterized by comprising a suspension of exosome lncRNA H19 derived from Mesenchymal Stem Cells (MSC), glycerol, isoamyl acetate, phosphatidylinositol-4, 5-bisphosphate 3-kinase and a hydrosolvent, and specifically comprises 20-30 parts of exosome lncRNA H19 suspension derived from Mesenchymal Stem Cells (MSC), 20-30 parts of glycerol, 10-15 parts of isoamyl acetate, 5-10 parts of phosphatidylinositol-4, 5-bisphosphate 3-kinase and 45-50 parts of a hydrosolvent; fully fusing exosome lncRNA H19 suspension derived from mesenchymal stem cells MSC with glycerol to ensure that the temperature is always 26-30 ℃, centrifuging for 5min by a centrifuge to obtain supernatant as a product A, taking subnatant as a product B, mixing the product B with phosphatidylinositol-4, 5-bisphosphate 3-kinase to obtain a product C, and fully mixing the product A and the product C with isoamyl acetate, glycerol and a hydrosolvent to obtain the skin barrier repair liquid.
The mesenchymal stem cells are applied to promoting skin barrier regeneration; PTEN was upregulated by MicroRNA-152-3p to promote wound healing on the skin surface.
The detection result of the skin repair liquid of the invention is as follows:
1. and (3) appearance detection: fine, smooth and transparent; colorless, pH 6.8; measuring the stability by centrifugation at 2000 r/min; cold resistance (-5 ℃ to-10 ℃),
2. and (3) detection of health indexes:
the total number of bacteria is less than 10CFU/g mould and the total number of microzyme is less than 10CFU/g lead and less than 2PPM arsenic and less than 0.2PPM mercury and less than 0.05PPM mercury
3. And (3) detection of functional components:
(1) and (3) activity detection:
the method comprises the following steps: three parts of appropriate skin repairing liquid are respectively taken to measure the initial activity, then the skin repairing liquid is heated in constant-temperature water bath at 39 +/-1 ℃, 44 +/-1 ℃, 49 +/-1 ℃ and 54 +/-1 ℃, and the skin repairing liquid is measured at intervals of 10 days at 39 +/-1 ℃,5 days at 44 +/-1 ℃, 1 day at 49 +/-1 ℃ and 1 hour at 54 +/-1 ℃.
(2) And (3) efficacy detection:
dividing 10 rats into two groups, namely a control group and an experimental group, and artificially damaging skin barriers on the backs of the rats by using a method including ultraviolet irradiation, bacterial intervention and the like;
the prepared skin repair liquid is smeared on the rats of the experimental group, and the skin barrier problem of the back skin of the rats of the experimental group, 4/5 is improved after observation for 60-90 days;
the rats in the control group are normally coated with glycerin, moisturizing milk and the like, and the skin barrier problem of the rats in the control group is not improved after observation for 60-90 days.
Example 2
As shown in fig. 1, an applying device of skin barrier repair liquid is characterized in that: comprises a main body 1, an applying main body 2 and a cover body 3; the front end of the main body 1 is provided with the smearing main body 2, the cover body 3 is arranged outside the smearing main body 2, a power supply assembly and a control assembly are arranged inside the main body 1, the repair liquid is controlled to be extruded back and forth by the liquid pushing rod 43, the repair liquid flows out of the smearing main body 2 and is smeared, and the smearing main body 2 is provided with the massage end 22 to assist the smearing main body 2 in implementing a massage function at the same time.
As shown in fig. 2, the main body 1 comprises a handle end 11 and an applying end 12, wherein the applying end 12 is integrated with the handle end 11 and is internally provided with a cavity; an external port 111 is arranged at the bottom of the handle end 11 and used for externally connecting a cosmetic instrument, and a switch button 121, a first reciprocating button 122 and a second reciprocating button 123 are arranged at the junction of the smearing end 12 and the handle end 11 and used for controlling the reciprocating motion of the liquid pushing rod 43; a liquid viewing window 124 is arranged at the middle lower part of the smearing end 12, is a transparent window and is used for observing the use state of the internal repairing liquid; the front end of the smearing end 12 is provided with a first concave ring 125.
As shown in fig. 3-4, the smearing main body 2 includes a liquid outlet plate 21, a massaging end 22 and a massaging brush 23, wherein the center of the liquid outlet plate 21 is provided with a plurality of liquid outlet holes 211 with different sizes, specifically, the outer ring of the liquid outlet holes is larger than the inner ring of the liquid outlet holes, and a plurality of liquid outlet holes 211 are arranged in each ring; the massage ends 22 are arranged on the periphery of the liquid outlet hole 211, the massage brush 23 is embedded or bonded at the bottom of the massage end 22, the massage ends 22 are three strip-shaped massage heads with radian, the periphery of the liquid outlet hole 211 plays a role in collecting repair liquid to prevent waste, and an auxiliary liquid channel 24 is arranged between every two massage ends 22 to prevent the unsatisfactory smearing effect caused by excessive accumulation of central repair liquid; the massage end 22 is provided with a plurality of massage control components, specifically, a massage base 221, a vibration area 222, a power control area 223 and a pressure sensing area 224, wherein the vibration area 222 is disposed below the power control area 223, the pressure sensing area 224 is disposed above the power control area 223, and the massage base 221 is disposed outside the vibration area 222, the power control area 223 and the pressure sensing area 224 as a housing.
The pressure sensing area 224 is a DFRobot resistance type pressure sensor; the vibration region 222 employs a model DFRobot FIT0774 mini vibration motor.
A second concave ring 31 is arranged on the periphery of the inner part of the cover body 3, a magnetic stone is embedded in the second concave ring 31 and mutually attracted with the magnetic stone in a first concave ring 125 arranged at the front end of the smearing main body 2 to assist in closing the cover body 3; the magnet is the same as the first concave ring 125 and the second concave ring 31.
As shown in fig. 5 to 6, a power supply assembly and a control assembly are arranged inside the main body 1, the power supply assembly is mainly a power supply 41, and includes an upper fixing member 411, a power supply main body 412 and a lower fixing member 412, the upper fixing member 411 is arranged above the power supply main body 412, the lower fixing member 412 is arranged below the power supply main body 412, the two fixing members fix the power supply main body 412 inside the handle end 11 of the main body 1 through a fixing nut assembly 414, and a second connecting end 415 is arranged on one side of the power supply main body 412 and used for connecting a first connecting end 421; the control assembly comprises a liquid pushing rod fixer 42, a liquid pushing rod 43 and a liquid pushing plate 44, wherein the upper end of the liquid pushing rod 43 is fixed inside the main body 1 through the liquid pushing rod fixer 42, the lower end of the liquid pushing rod 43 is directly connected with the liquid pushing plate 44, a control area is arranged inside the liquid pushing rod fixer 42, and the control area extends out of the control area and is used for being connected with the second connecting end 415.
The liquid pushing rod 43 is a mntg miniature reciprocating pushing rod motor.
As a possible implementation manner, when the liquid dispenser is used, the first reciprocating button 122 and the second reciprocating button 123 are controlled to discharge liquid, and the liquid using condition can be observed through the liquid window; when the patch is used, the applied part is placed on the skin surface, and the pressure sensing area 224 senses the pressure to release a signal to the power control area 223, so as to control the vibration area 222 to vibrate, massage and promote the absorption of the repair liquid.
As a possible implementation, the center of the main body 1 of the present embodiment can be separated, so as to facilitate the replacement of liquid.
As a possible implementation manner, the external connection port 111 of the present embodiment may be used for connecting an external instrument, or may be used directly as a product application device without connecting any instrument.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the preferred embodiments of the invention and described in the specification are only preferred embodiments of the invention and are not intended to limit the invention, and that various changes and modifications may be made without departing from the novel spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A method for promoting skin barrier regeneration by over-expressing lncRNA H19 in mesenchymal stem cells is characterized by comprising a skin barrier repair liquid and an application device of the skin barrier repair liquid, wherein the application device is used for applying the skin barrier repair liquid to the damaged part of the skin barrier while performing punch type massage application.
2. The method for promoting skin barrier regeneration by overexpressing lncRNA H19 in the mesenchymal stem cells according to claim 1, wherein the skin barrier repair solution comprises a suspension of Mesenchymal Stem Cell (MSC) -derived exosome lncRNA H19, glycerol, isoamyl acetate, phosphatidylinositol-4, 5-bisphosphate 3-kinase and an aqueous solvent, specifically 20-30 parts of suspension of Mesenchymal Stem Cell (MSC) -derived exosome lncRNA H19, 20-30 parts of glycerol, 10-15 parts of isoamyl acetate, 5-10 parts of phosphatidylinositol-4, 5-bisphosphate 3-kinase and 45-50 parts of an aqueous solvent; fully fusing exosome lncRNA H19 suspension derived from Mesenchymal Stem Cells (MSC) with glycerol to ensure that the temperature is 26-30 ℃ all the time, centrifuging for 5min by a centrifugal machine to obtain supernatant as a product A and lower liquid as a product B, mixing the product B with phosphatidylinositol-4, 5-bisphosphate 3-kinase to obtain a product C, and fully mixing the product A and the product C with isoamyl acetate, glycerol and a hydrosolvent to obtain the skin barrier repair liquid.
3. The skin barrier repair fluid according to claim 2, wherein: the mesenchymal stem cells are applied to promoting skin barrier regeneration; PTEN was upregulated by MicroRNA-152-3p to promote wound healing on the skin surface.
4. The method for promoting skin barrier regeneration by over-expressing lncRNA H19 in the mesenchymal stem cells according to claim 1, wherein the device for applying the skin barrier repair solution comprises a main body (1), an applying main body (2) and a cover body (3); the utility model discloses a massage liquid medicine applying device, including main part (1), main part (2), lid (3), power supply module and control assembly, control liquid pushing rod (43) reciprocal extrusion repair liquid are equipped with to main part (1) front end the main part (2) is paintd, it is equipped with to paint main part (2) outward the lid (3), main part (1) inside is equipped with power supply module and control assembly, follow it paints to paint main part (2) outflow, it is equipped with massage end (22) and assists to paint main part (2) and implement the massage function simultaneously.
5. The application device of skin barrier repair liquid according to claim 4, wherein: the main body (1) comprises a handle end (11) and an applying end (12), the applying end (12) and the handle end (11) are integrated, and a cavity is formed inside the applying end (12); an external port (111) is formed in the bottom of the handle end (11), and a switch button (121), a first reciprocating button (122) and a second reciprocating button (123) are arranged at the junction of the smearing end (12) and the handle end (11) and used for controlling the reciprocating motion of the liquid pushing rod (43); a liquid viewing window (124) is arranged at the middle lower part of the smearing end (12), is a transparent window and is used for observing the use state of the internal repairing liquid; the front end of the smearing end (12) is provided with a first concave ring (125).
6. The application device of skin barrier repair liquid according to claim 4, wherein: the front end of the main body (1) and the tail end of the smearing main body (2) are provided with spiral openings.
7. The application device of skin barrier repair liquid according to claim 4, wherein: the smearing main body (2) comprises a liquid outlet plate (21), a massage end (22) and a massage brush (23), wherein the center of the liquid outlet plate (21) is provided with a plurality of circles of liquid outlet holes (211) with different sizes, specifically, the outer circle of holes are larger than the inner circle of holes, and a plurality of liquid outlet holes (211) are arranged in each circle; the periphery of the liquid outlet hole (211) is provided with the massage end (22), the bottom of the massage end (22) is embedded or bonded with the massage brush (23), the massage end (22) is provided with three strip-shaped massage heads with radian, and an auxiliary liquid channel (24) is arranged between every two massage ends (22); massage end (22) inside is equipped with a plurality of massage control assembly, specifically is massage base (221), vibrations district (222), power control district (223) and pressure sensing district (224), vibrations district (222) are located power control district (223) below, power control district (223) top is equipped with pressure sensing district (224), massage base (221) are arranged in as the shell the outside in vibrations district (222), power control district (223) and pressure sensing district (224).
8. The application device of skin barrier repair liquid according to claim 4, wherein: a second concave ring (31) is arranged on the periphery of the inner part of the cover body (3), a magnetic stone is embedded in the second concave ring (31) and mutually attracted with the magnetic stone in a first concave ring (125) arranged at the front end of the smearing main body (2) to assist in closing the cover body (3); the magnetic stone has the same shape and size with the first concave ring (125) and the second concave ring (31).
9. The application device of skin barrier repair liquid according to claim 4, wherein: the power supply assembly and the control assembly are arranged in the main body (1), the power supply assembly is mainly a power supply (41) and comprises an upper end fixing piece (411), a power supply main body (412) and a lower end fixing piece (412), the upper end fixing piece (411) is arranged above the power supply main body (412), the lower end fixing piece (412) is arranged below the power supply main body (412), the upper end fixing piece and the lower end fixing piece fix the power supply main body (412) in the handle end (11) of the main body (1) through a fixing nut assembly (414), and a second connecting end (415) is arranged on one side of the power supply main body (412) and used for being connected with a first connecting end (421); the control assembly comprises a liquid pushing rod fixer (42), a liquid pushing rod (43) and a liquid pushing plate (44), wherein the upper end of the liquid pushing rod (43) is fixed inside the main body (1) through the liquid pushing rod fixer (42), the lower end of the liquid pushing rod (43) is directly connected with the liquid pushing plate (44), a control area is arranged inside the liquid pushing rod fixer (42), and the control area extends out of the liquid pushing rod fixer and is used for being connected with the second connecting end (415).
10. The application method of the smearing device for the skin barrier repair liquid is characterized by comprising the following steps: the method comprises the following steps:
s1: adding a skin barrier repair liquid into the main body (1);
s2: controlling a first reciprocating button (122) and a second reciprocating button (123) to discharge liquid, and observing the use condition of the liquid through a liquid viewing window;
s3: the applied part is placed on the surface of the skin, and the pressure sensing area (224) senses the pressure to release a signal to the power control area (223) at the moment, so that the vibration area (222) is controlled to vibrate, massage is carried out, and the absorption of the repair liquid is promoted.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210136495.5A CN114522134A (en) | 2022-02-15 | 2022-02-15 | Method for promoting skin barrier regeneration by over-expressing lncRNA H19 in mesenchymal stem cells |
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| CN202210136495.5A CN114522134A (en) | 2022-02-15 | 2022-02-15 | Method for promoting skin barrier regeneration by over-expressing lncRNA H19 in mesenchymal stem cells |
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