CN114515447B - Blood separation device, working method and PRP plasma separation method - Google Patents

Blood separation device, working method and PRP plasma separation method Download PDF

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Publication number
CN114515447B
CN114515447B CN202210049677.9A CN202210049677A CN114515447B CN 114515447 B CN114515447 B CN 114515447B CN 202210049677 A CN202210049677 A CN 202210049677A CN 114515447 B CN114515447 B CN 114515447B
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separation
pipe
tube
injection
inner cavity
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CN114515447A (en
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李长春
高春强
胡艳灵
王海龙
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Changzhou Weierkang Medical Technology Co ltd
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Changzhou Weierkang Medical Technology Co ltd
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D17/00Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02Separation of non-miscible liquids

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  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • External Artificial Organs (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to a blood separating device, which comprises an injection tube, wherein the upper end of the injection tube is connected with a first separating tube, and the lower end of the injection tube is connected with a second separating tube; the first separation pipe and the second separation pipe comprise a pipe body and a cover body, and the on-off mechanism is arranged between the pipe body and the injection pipe. A method of operation comprising a first separation of blood after closure of a lumen of a first separation tube; and filling the serum in the injection tube into the second separation tube for secondary separation, and extracting PRP plasma through the pumping hole of the second cover body. A PRP plasma separation method comprising a first separation of blood after a first separation tube lumen is closed; and filling the serum in the injection tube into the second separation tube for secondary separation, and extracting PRP plasma through the pumping hole of the second cover body. The internal space of the injection pipe is not occupied, and the communication of the cavity between the separation pipe and the injection pipe can be controlled stably and reliably.

Description

Blood separation device, working method and PRP plasma separation method
Technical Field
The invention relates to a blood separation device, a working method and a PRP plasma separation method.
Background
At present, a blood separation device is used for separating PRP plasma in blood, and the blood is separated twice sequentially by the blood separation device, so that the PRP plasma is separated.
Existing blood separation devices refer to the prior patent, patent No.: 201280063492.8, patent name: in a conventional blood separation device, an opening of an upper separation chamber and a lower separation chamber is blocked by a stopper having a plunger rod type structure, and the following problems are found in a conventional plunger type device:
The plunger rod is screwed down through the screw, and the plunger rod has the fracture risk to owing to set up the plunger rod in the intermediate chamber, influence blood easily and circulate before two upper and lower separation chamber, influence the volume of intermediate chamber.
Therefore, in summary, the existing blood separation device has the problems that the plugging structure is unreliable, and the leakage of the upper separation chamber and the lower separation chamber is easy to occur, so that the stable and reliable blood separation operation of the blood separation device is affected.
Disclosure of Invention
The invention aims to solve the technical problems that: the defects of the prior art are overcome, the blood separation device, the working method and the PRP plasma separation method are provided, and the problems that the plugging structure of the existing blood separation device is unstable and unreliable and the separation chamber is easy to leak are solved.
The technical scheme adopted for solving the technical problems is as follows:
First aspect:
There is provided a blood separation device comprising
The upper end of the injection pipe is connected with the first separation pipe, and the lower end of the injection pipe is connected with the second separation pipe;
the first separating pipe and the second separating pipe each comprise
The injection tube comprises a tube body and a cover body, wherein one end of the tube body is in threaded connection with the injection tube, and the other end of the tube body is in threaded connection with the cover body; the cover body and the pipe body relatively rotate so as to adjust the inner cavity volume of the separation pipe;
The on-off mechanism is arranged between the pipe body and the injection pipe and is suitable for controlling the on-off of the inner cavity of the injection pipe and the inner cavity of the separation pipe; the tube body and the injection tube relatively rotate, and the on-off mechanism controls the on-off between the inner cavity of the injection tube and the inner cavity of the separation tube.
Further, the on-off mechanism comprises
The first sealing surface is formed on the injection pipe, a first through hole is formed in the first sealing surface, and the first through hole is communicated with the inner cavity of the injection pipe;
The second sealing surface is formed on the inner pipe, a second through hole is formed in the second sealing surface, and the second through hole is communicated with the inner cavity of the pipe body;
When the first sealing surface and the second sealing surface are tightly screwed together with the injection pipe and the inner pipe to form sealing and abutting, the first through hole and the second through hole are both in a blocking state so as to disconnect the inner cavity of the injection pipe from the inner cavity of the pipe body;
When the first sealing surface and the second sealing surface are loosened along with the injection pipe and the inner pipe so as to separate the first sealing surface from the second sealing surface, the inner pipe of the injection pipe and the inner pipe cavity are communicated through a separation cavity between the two sealing surfaces.
Further, the injection tube comprises
The upper end of the upper pipe is connected with the first separation pipe, the lower end of the upper pipe is connected with the upper end of the lower pipe in a threaded manner, and the lower end of the lower pipe is connected with the second separation pipe in a threaded manner.
Further, a drawing hole is formed in the cover body of the second separation tube.
Further, a sealing boss is formed on the second sealing surface of the inner tube, and the sealing boss is suitable for being in sealing fit with the first through hole.
Second aspect:
Provides a working method of the blood separating device, which comprises
The blood passes through an injection inlet of the injection tube and is filled into the first separation tube, after the inner cavity of the first separation tube is closed, the first separation is carried out, after the separation, red blood cells are positioned at the lower layer, serum is positioned at the upper layer, the serum is pushed into the injection tube by rotating a first cover body of the first separation tube, then the first separation tube is closed, and the red blood cells are left in the first separation tube;
And filling serum in the injection tube into the second separation tube, sealing the inner cavity of the second separation tube, performing secondary separation, forming a PRP layer and an impurity layer after separation, pushing the impurity layer into the injection tube by rotating a second cover body of the second separation tube, sealing the second separation tube, and extracting PRP plasma through a drawing hole of the second cover body.
Third aspect:
a PRP plasma separation method is provided, which is characterized in that the blood separation device comprises
The blood passes through an injection inlet of the injection tube and is filled into the first separation tube, after the inner cavity of the first separation tube is closed, the first separation is carried out, after the separation, red blood cells are positioned at the lower layer, serum is positioned at the upper layer, the serum is pushed into the injection tube by rotating a first cover body of the first separation tube, then the first separation tube is closed, and the red blood cells are left in the first separation tube;
And filling serum in the injection tube into the second separation tube, sealing the inner cavity of the second separation tube, performing secondary separation, forming a PRP layer and an impurity layer after separation, pushing the impurity layer into the injection tube by rotating a second cover body of the second separation tube, sealing the second separation tube, and extracting PRP plasma through a drawing hole of the second cover body.
The beneficial effects of the invention are as follows:
According to the blood separation device, the working method and the PRP plasma separation method, the on-off mechanism between the inner cavities of the upper separation tube and the lower separation tube and the injection tube is designed again, the inner space of the injection tube is not occupied, the whole assembly is convenient, all the components are connected by screw threads, the risk of fracture does not exist, the communication between the separation tube and the injection tube can be controlled stably and reliably, no other components exist in the injection tube, and the plasma can flow smoothly up and down.
Drawings
The invention is further described below with reference to the accompanying drawings.
FIG. 1 is a perspective view of a blood separation device of the present invention;
FIG. 2 is a semi-sectional view of a blood separation device of the present invention;
FIG. 3 is a mating view of an injection tube and a first separation tube;
Wherein, 1, an injection pipe, 11, an upper pipe, 12 and a lower pipe;
2. a first separating tube 21, a first inner tube 22, and a first cover;
3. a second separation pipe 31, a second inner pipe 32 and a second cover body;
4. The on-off mechanism, 41, the first through hole, 42, the second through hole, 421 and the sealing boss.
Detailed Description
The invention will now be further described with reference to the accompanying drawings. These drawings are simplified schematic views illustrating the basic structure of the present invention by way of illustration only, and thus show only the constitution related to the present invention.
Example 1
As shown in FIG. 1 and FIG. 2, a blood separation device includes
The upper end of the injection pipe 1 is connected with the first separation pipe 2, the lower end of the injection pipe is connected with the second separation pipe 3, and a breather valve and an injection port are arranged on the injection pipe 1;
the first separating tube 2 comprises a first tube body and a first cover body 22, wherein one end of the first tube body is in threaded connection with the injection tube 1, and the other end of the first tube body is in threaded connection with the first cover body 22; the first cover 22 and the first pipe body relatively rotate to adjust the inner cavity volume of the first separation pipe 2;
The second separation pipe 3 comprises a second pipe body and a second cover body 32, one end of the second pipe body is in threaded connection with the injection pipe 1, and the other end of the second pipe body is in threaded connection with the second cover body 32; the second cover 32 and the second pipe body relatively rotate to adjust the inner cavity volume of the second separation pipe 3; and a second cover 32 of the second separation tube 3 is provided with a drawing hole;
An on-off mechanism 4 is arranged between the first separation pipe 2 and the injection pipe 1 and between the second separation pipe 3 and the injection pipe 1; the on-off mechanism 4 is used for controlling the on-off of the inner cavity of the injection pipe 1 and the inner cavity of the separation pipe;
the pipe body in the separation pipe and the injection pipe 1 are rotated relatively, and the on-off between the inner cavity of the injection pipe 1 and the inner cavity of the separation pipe is controlled by the on-off mechanism 4.
Specifically, as an alternative implementation of the present embodiment, as shown in fig. 2, the on-off mechanism 4 includes
The first sealing surface is formed on the injection pipe 1, a threaded connection section is formed on the injection pipe 1, the first sealing surface is positioned at the front end of the threaded connection section, a first through hole 41 is formed in the first sealing surface, and the first through hole 41 is communicated with the inner cavity of the injection pipe 1;
the second sealing surface is formed on the inner pipe (the first inner pipe or the second inner pipe), a threaded connecting hole is formed in the inner pipe, the second sealing surface is positioned at the bottom surface of the threaded connecting hole, a second through hole 42 is formed in the second sealing surface, and the second through hole 42 is communicated with the inner cavity of the pipe body;
When the first sealing surface and the second sealing surface are screwed together between the injection pipe 1 and the inner pipe to form sealing and abutting, the first through hole 41 and the second through hole 42 are in a blocking state so as to disconnect the inner cavity of the injection pipe 1 and the inner cavity of the pipe body;
When the first sealing surface and the second sealing surface are loosened along with the separation between the injection pipe 1 and the inner pipe so as to separate the first sealing surface from the second sealing surface, the inner pipe of the injection pipe 1 and the inner pipe cavity are communicated through a separation cavity between the two sealing surfaces.
The inner tube in this embodiment may be the first inner tube 21 or the second inner tube 31, and the principles of the on-off mechanism 4 of the first separation tube 2 and the second separation tube 3 are the same.
Unlike the prior patent, the on-off mechanism 4 of the embodiment does not occupy the inner cavity space of the injection tube 1, and is reliable and stable in plugging the injection tube 1.
Specifically, as an alternative implementation manner in this embodiment, as shown in fig. 3, a sealing boss 421 is formed on the second sealing surface of the inner tube, and the sealing boss 421 is adapted to form a sealing fit with the first through hole 41. By means of the sealing boss 421, the tightness between the inner tube of the elevator and the injection tube 1 is improved.
Specifically, as an alternative in this example, as shown in fig. 1, the injection tube 1 includes
The upper end of the upper pipe 11 is connected with the first separation pipe 2, the lower end of the upper pipe is in threaded connection with the upper end of the lower pipe 12, and the lower end of the lower pipe 12 is in threaded connection with the second separation pipe 3.
All parts in the blood separation device are connected together by adopting threads, so that the blood separation device is convenient to assemble, particularly, the design of the on-off mechanism 4 does not occupy the inner cavity of the injection tube 1, the on-off control between the separation tube and the injection tube 1 is stable and reliable, and the separation of PRP plasma is ensured.
The working method of the blood separation device of the invention is as follows:
the blood passes through the injection inlet of the injection tube 1 and is filled into the first separation tube 2, after the inner cavity of the first separation tube 2 is closed, the first separation is carried out, after the separation, the red blood cells are positioned at the lower layer, the serum is positioned at the upper layer, the serum is pushed into the injection tube 1 by rotating the first cover 22 of the first separation tube 2, then the first separation tube 2 is closed, and the red blood cells are left in the first separation tube 2;
Serum in the injection tube 1 is filled into the second separation tube 3, the inner cavity of the second separation tube 3 is sealed and then subjected to secondary separation, a PRP layer and an impurity layer are formed after separation, the impurity layer is pushed into the injection tube 1 by rotating the second cover body 32 of the second separation tube 3, then the second separation tube 3 is sealed, and PRP plasma is extracted through the pumping hole of the second cover body 32.
Example two
A PRP plasma separation method, which adopts the blood separation device, comprises
The blood passes through the injection inlet of the injection tube 1 and is filled into the first separation tube 2, after the inner cavity of the first separation tube 2 is closed, the first separation is carried out, after the separation, the red blood cells are positioned at the lower layer, the serum is positioned at the upper layer, the serum is pushed into the injection tube 1 by rotating the first cover 22 of the first separation tube 2, then the first separation tube 2 is closed, and the red blood cells are left in the first separation tube 2;
Serum in the injection tube 1 is filled into the second separation tube 3, the inner cavity of the second separation tube 3 is sealed and then subjected to secondary separation, a PRP layer and an impurity layer are formed after separation, the impurity layer is pushed into the injection tube 1 by rotating the second cover body 32 of the second separation tube 3, then the second separation tube 3 is sealed, and PRP plasma is extracted through the pumping hole of the second cover body 32.
With the above-described preferred embodiments according to the present invention as an illustration, the above-described descriptions can be used by persons skilled in the relevant art to make various changes and modifications without departing from the scope of the technical idea of the present invention. The technical scope of the present invention is not limited to the description, but must be determined according to the scope of claims.

Claims (5)

1. A blood separating device, characterized by comprising
The upper end of the injection pipe (1) is connected with the first separation pipe (2), and the lower end of the injection pipe is connected with the second separation pipe (3);
The first separation pipe (2) and the second separation pipe (3) comprise
The injection device comprises a pipe body and a cover body, wherein one end of the pipe body is in threaded connection with an injection pipe (1), and the other end of the pipe body is in threaded connection with the cover body; the cover body and the pipe body relatively rotate so as to adjust the inner cavity volume of the separation pipe;
The on-off mechanism (4) is arranged between the pipe body and the injection pipe (1) and is suitable for controlling the on-off of the inner cavity of the injection pipe (1) and the inner cavity of the separation pipe; the pipe body and the injection pipe (1) rotate relatively, and the on-off mechanism (4) controls the on-off between the inner cavity of the injection pipe (1) and the inner cavity of the separation pipe;
The on-off mechanism (4) comprises
The first sealing surface is formed on the injection pipe (1), a first through hole (41) is formed in the first sealing surface, and the first through hole (41) is communicated with the inner cavity of the injection pipe (1);
the second sealing surface is formed on the inner pipe, a second through hole (42) is formed in the second sealing surface, and the second through hole (42) is communicated with the inner cavity of the pipe body;
when the first sealing surface and the second sealing surface are screwed together between the injection pipe (1) and the inner pipe to form sealing and propping, the first through hole (41) and the second through hole (42) are in a blocking state so as to disconnect the inner cavity of the injection pipe (1) and the inner cavity of the pipe body;
when the first sealing surface and the second sealing surface are loosened along with the separation between the injection pipe (1) and the inner pipe so as to separate the first sealing surface from the second sealing surface, the inner pipe of the injection pipe (1) and the inner pipe cavity are communicated through a separation cavity between the two sealing surfaces;
the injection tube (1) comprises
The device comprises an upper pipe (11) and a lower pipe (12), wherein the upper end of the upper pipe (11) is connected with the first separation pipe (2), the lower end of the upper pipe is in threaded connection with the upper end of the lower pipe (12), and the lower end of the lower pipe (12) is in threaded connection with the second separation pipe (3).
2. A blood separation device according to claim 1, wherein,
And a drawing hole is formed in the cover body of the second separation tube (3).
3. A blood separation device according to claim 1, wherein,
A sealing boss (421) is formed on the second sealing surface of the inner tube, and the sealing boss (421) is suitable for being in sealing fit with the first through hole (41).
4. A method of operating a blood separation device according to any one of claims 1 to 3, wherein: comprising
The blood passes through an injection inlet of the injection tube (1) and is filled into the first separation tube (2), after the inner cavity of the first separation tube (2) is closed, the first separation is carried out, after the separation, red blood cells are positioned at the lower layer, serum is positioned at the upper layer, the serum is pushed into the injection tube (1) by rotating a first cover body (22) of the first separation tube (2), then the first separation tube (2) is closed, and the red blood cells are left in the first separation tube (2);
Serum in the injection tube (1) is filled into the second separation tube (3), the inner cavity of the second separation tube (3) is sealed and then separated for the second time, a PRP layer and an impurity layer are formed after separation, the impurity layer is pushed into the injection tube (1) by rotating a second cover body (32) of the second separation tube (3), then the second separation tube (3) is sealed, and PRP plasma is extracted through a pumping hole of the second cover body (32).
5. A PRP plasma separation method, characterized by using the blood separation device according to any one of claims 1 to 3, comprising
The blood passes through an injection inlet of the injection tube (1) and is filled into the first separation tube (2), after the inner cavity of the first separation tube (2) is closed, the first separation is carried out, after the separation, red blood cells are positioned at the lower layer, serum is positioned at the upper layer, the serum is pushed into the injection tube (1) by rotating a first cover body (22) of the first separation tube (2), then the first separation tube (2) is closed, and the red blood cells are left in the first separation tube (2);
Serum in the injection tube (1) is filled into the second separation tube (3), the inner cavity of the second separation tube (3) is sealed and then separated for the second time, a PRP layer and an impurity layer are formed after separation, the impurity layer is pushed into the injection tube (1) by rotating a second cover body (32) of the second separation tube (3), then the second separation tube (3) is sealed, and PRP plasma is extracted through a pumping hole of the second cover body (32).
CN202210049677.9A 2022-01-17 2022-01-17 Blood separation device, working method and PRP plasma separation method Active CN114515447B (en)

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CN114515447B true CN114515447B (en) 2024-05-28

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114870436B (en) * 2022-06-21 2024-08-06 浙江保尔曼生物科技有限公司 Platelet-rich plasma preparation device and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101172207A (en) * 2007-10-12 2008-05-07 经建中 Separator disk on multi-cell component mix liquid separating system and application method of the same
CN101298008A (en) * 2008-01-14 2008-11-05 经建中 Pipe coil structure applied on mixed liquid continuous centrifugal separation system
TWM466716U (en) * 2013-06-11 2013-12-01 Yung Kang Medical Device Technologic Co Ltd Concentrator for separating autologous blood

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101172207A (en) * 2007-10-12 2008-05-07 经建中 Separator disk on multi-cell component mix liquid separating system and application method of the same
CN101298008A (en) * 2008-01-14 2008-11-05 经建中 Pipe coil structure applied on mixed liquid continuous centrifugal separation system
TWM466716U (en) * 2013-06-11 2013-12-01 Yung Kang Medical Device Technologic Co Ltd Concentrator for separating autologous blood

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