CN114507280A - Preparation method of enzymolysis ovalbumin - Google Patents
Preparation method of enzymolysis ovalbumin Download PDFInfo
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- CN114507280A CN114507280A CN202210229309.2A CN202210229309A CN114507280A CN 114507280 A CN114507280 A CN 114507280A CN 202210229309 A CN202210229309 A CN 202210229309A CN 114507280 A CN114507280 A CN 114507280A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/77—Ovalbumin
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
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- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21004—Trypsin (3.4.21.4)
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- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22002—Papain (3.4.22.2)
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Abstract
The invention discloses a preparation method of enzymolysis ovalbumin, belonging to the technical field of enzymolysis ovalbumin production, wherein the enzymolysis ovalbumin is composed of the following raw materials in parts by weight: 0.21-0.51 part of ornithine, 1.50-6.00 parts of lysine, 0.80-1.80 parts of histidine, 2.25-5.50 parts of arginine, 0.80-3.80 parts of threonine, 1.50-6.00 parts of serine, 1.41-8.24 parts of glutamic acid, 1.00-2.58 parts of proline, 0.30-2.98 parts of glycine, 1.86-5.17 parts of alanine and the balance of water.
Description
Technical Field
The invention relates to the technical field of enzymolysis ovalbumin production, in particular to a preparation method of enzymolysis ovalbumin.
Background
The egg white is subjected to biochemical enzymolysis by modern high technology to obtain micromolecule polypeptide with strong biological activity, namely enzymolysis ovalbumin, and the polypeptide has different functions on human bodies due to different composition structures, so that the functions of inhibition, activation, restoration and promotion are summarized, wherein the inhibition is the inhibition of cytopathic effect, and the immunity of the human bodies is enhanced; the activation is to activate the cell activity and eliminate the free radical harmful to the human body; repairing cells with pathological changes of human body and improving metabolism.
Through retrieval, chinese patent No. CN202010873922.9 discloses an egg albumin zymolyte, a preparation method and an application thereof, although the zymolytic egg albumin can be prepared, enzymes used in the preparation process are all disposable consumables and cannot be recycled, so that the preparation cost is increased, meanwhile, the control of the enzyme catalysis process is difficult, and the recovery of general immobilized enzymes is complicated, so that time and labor are wasted, the production efficiency of the zymolytic egg albumin is affected, and the problem of method defects is caused.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a preparation method of enzymolysis ovalbumin.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of enzymolysis ovalbumin comprises the following raw materials in parts by weight: 0.21-0.51 part of ornithine, 1.50-6.00 parts of lysine, 0.80-1.80 parts of histidine, 2.25-5.50 parts of arginine, 0.80-3.80 parts of threonine, 1.50-6.00 parts of serine, 1.41-8.24 parts of glutamic acid, 1.00-2.58 parts of proline, 0.30-2.98 parts of glycine, 1.86-5.17 parts of alanine, 2.20-5.20 parts of cystine, 3.32-8.03 parts of valine, 4.34-8.03 parts of leucine, 1.17-3.86 parts of isoleucine, 0.41-1.44 parts of tyrosine, 2.25-7.33 parts of phenylalanine, 1.03-2.44 parts of aspartic acid, 2.49-8.40 parts of methionine and the balance of water; the preparation method of the enzymolysis ovalbumin comprises the following specific steps:
the method comprises the following steps: obtaining egg white: soaking the collected eggs until impurities on the outer surfaces of the eggs are separated from the eggs, then fishing out the eggs, washing the eggs with clear water, sterilizing with sterile water, crushing the eggs under the aseptic condition of 4-6 ℃, then separating egg white from yolk, keeping the egg white, finally treating the egg white by a pasteurization method, and cooling to 4 ℃ to obtain egg white;
step two: preparing an immobilized enzyme: mixing papain and trypsin to form mixed enzyme, preparing a mixed enzyme carrier, and carrying out covalent connection on the mixed enzyme carrier and the mixed enzyme to obtain an immobilized enzyme;
step three: enzymolysis: weighing the egg white in the first step, adding water, stirring into emulsion, adjusting the pH value and temperature to form a reaction substrate, putting the immobilized enzyme in the second step into the reaction substrate for hydrolysis reaction for 6-8h, and obtaining an enzymolysis solution after the reaction is finished;
step four: and (3) recovering the immobilized enzyme: electrifying the electromagnet, putting the electromagnet into the enzymolysis solution for stirring, enabling the electromagnet to adsorb the immobilized enzyme in the enzymolysis solution, taking out the electromagnet, powering off the electromagnet, enabling the immobilized enzyme adsorbed on the electromagnet to fall off, repeating the process until no immobilized enzyme is adsorbed, and completing the recovery of the immobilized enzyme;
step five: centrifugal screening: heating the enzymolysis solution obtained in the fourth step to 90-95 ℃, preserving heat for 20-30min, cooling to room temperature to passivate mixed enzyme remaining in the enzymolysis solution, placing the enzymolysis solution into a centrifuge for centrifugal separation to form supernatant and residues, removing the residues, and retaining the supernatant to obtain enzymolysis ovalbumin liquid;
step six: and (3) post-treatment: sterilizing, concentrating and drying the enzymolysis ovalbumin liquid to obtain the enzymolysis ovalbumin.
Further, the pasteurization in the step one is carried out at the temperature of 60-70 ℃, the heat preservation time is 0.25-0.5h, and the cooling time is 2-3 min.
Further, the specific operation of preparing the mixed enzyme carrier in the step two is as follows:
s1, sequentially putting the multi-walled carbon nanotube, the nano titanium dioxide and the ferroferric oxide powder into grinding equipment, grinding and stirring the raw materials to fully mix the raw materials to obtain mixed powder, drying the mixed powder, dispersing the mixed powder into a polyethylene glycol aqueous solution, dropwise adding vinyltriethoxysilane, stirring the mixture by using stirring equipment, finally adding a tetramethyl orthosilicate solution for mixing, and obtaining a mixed solution after the mixing is finished;
s2, mixing liquid paraffin and trichloromethane, adding an emulsifier after mixing, continuously stirring, keeping the temperature at 60 ℃ all the time in the process, adding the mixed solution while stirring the emulsifier, stirring for 0.5 hour again, cooling to room temperature, and centrifuging in a centrifuge to form a supernatant and a pre-carrier;
s3, removing the supernatant in the step S2, reserving the pre-carrier, cleaning the pre-carrier by ethyl acetate, and finally washing by clear water to obtain the mixed enzyme carrier.
Further, the mixed powder is dispersed in the step S1 by an ultrasonic dispersion method, the dispersion time is 0.5-1h, and the dispersion temperature is 50 ℃.
Further, the pH and temperature in step three are suitably 6-8 and 36-40 deg.C, respectively.
Further, the magnetic field intensity of the electromagnet in the fourth step is 0.4-0.6T.
Further, the rotation speed of the centrifuge in the step five is 4000-.
Compared with the prior art, the invention has the beneficial effects that:
1. the mixed enzyme is processed into the immobilized enzyme, so that the storage period of the mixed enzyme is prolonged, the enzymolysis process is more controllable, the pure mixed enzyme is inactivated or passivated in subsequent processing, only one processing can be completed, the immobilized enzyme can be recovered and can be repeatedly used, and the investment of the preparation cost is reduced.
2. According to the invention, the ferroferric oxide powder is added into the mixed enzyme carrier, so that the mixed enzyme carrier contains iron element, and the electromagnet can adsorb the immobilized enzyme, thereby simplifying the recovery of the immobilized enzyme, saving time and labor, and ensuring the production efficiency of the enzymolysis ovalbumin.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
FIG. 1 is a schematic production flow diagram of a preparation method of an enzymolysis ovalbumin provided by the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1:
referring to fig. 1, the present invention provides a technical solution: a preparation method of enzymolysis ovalbumin comprises the following raw materials in parts by weight: 0.21 part of ornithine, 1.50 parts of lysine, 0.80 part of histidine, 2.25 parts of arginine, 0.80 part of threonine, 1.50 parts of serine, 1.41 parts of glutamic acid, 1.00 part of proline, 0.30 part of glycine, 1.86 parts of alanine, 2.20 parts of cystine, 3.32 parts of valine, 4.34 parts of leucine, 1.17 parts of isoleucine, 0.41 part of tyrosine, 2.25 parts of phenylalanine, 1.03 part of aspartic acid and 2.49 parts of methionine, and the balance of water; the preparation method of the enzymolysis ovalbumin comprises the following specific steps:
the method comprises the following steps: obtaining egg white: soaking the collected eggs until impurities on the outer surfaces of the eggs are separated from the eggs, then fishing out the eggs, washing the eggs with clear water, sterilizing the eggs with sterile water, crushing the eggs under the aseptic condition of 4 ℃, then separating egg white from egg yolk, keeping the egg white, finally treating the egg white by a pasteurization method, and cooling to 4 ℃ to obtain the egg white, wherein the pasteurization temperature is 60 ℃, the heat preservation time is 0.25h, and the cooling time is 2 min;
step two: preparing an immobilized enzyme: mixing papain and trypsin to form mixed enzyme, preparing a mixed enzyme carrier, and carrying out covalent connection on the mixed enzyme carrier and the mixed enzyme to obtain the immobilized enzyme, wherein the specific operation of preparing the mixed enzyme carrier is as follows:
s1, sequentially putting the multi-walled carbon nanotube, the nano titanium dioxide and the ferroferric oxide powder into grinding equipment, grinding and stirring the raw materials to fully mix the raw materials to obtain mixed powder, drying the mixed powder, dispersing the mixed powder into a polyethylene glycol aqueous solution, dropwise adding vinyltriethoxysilane, stirring by using stirring equipment, finally adding a tetramethyl orthosilicate solution for mixing, and obtaining a mixed solution after mixing, wherein the mixed powder is dispersed by an ultrasonic dispersion method for 0.5h at a dispersion temperature of 50 ℃;
s2, mixing liquid paraffin and trichloromethane, adding an emulsifier after mixing, continuously stirring, keeping the temperature at 60 ℃ all the time in the process, adding the mixed solution while stirring the emulsifier, stirring for 0.5 hour again, cooling to room temperature, and centrifuging in a centrifuge to form a supernatant and a pre-carrier;
s3, removing the supernatant in the step S2, reserving the pre-carrier, cleaning the pre-carrier by using ethyl acetate, and finally washing by using clear water to obtain a mixed enzyme carrier;
step three: enzymolysis: weighing the egg white in the first step, adding water, stirring into emulsion, adjusting the pH value and the temperature to form a reaction substrate, putting the immobilized enzyme in the second step into the reaction substrate for hydrolysis reaction for 6 hours, and obtaining an enzymolysis solution after the reaction is finished, wherein the pH value and the temperature are respectively 6 and 36 ℃;
step four: and (3) recovering the immobilized enzyme: electrifying the electromagnet, putting the electromagnet into the enzymolysis solution, stirring to enable the electromagnet to adsorb the immobilized enzyme in the enzymolysis solution, taking out the electromagnet, powering off the electromagnet to enable the immobilized enzyme adsorbed on the electromagnet to fall off, repeating the process until no immobilized enzyme is adsorbed, and completing the recovery of the immobilized enzyme, wherein the magnetic field intensity of the electromagnet is 0.4T;
step five: centrifugal screening: heating the enzymolysis solution obtained in the fourth step to 90 ℃, preserving heat for 20min, cooling to room temperature to passivate mixed enzyme remaining in the enzymolysis solution, placing the enzymolysis solution into a centrifuge for centrifugal separation to form supernatant and residue, removing the residue and retaining the supernatant, namely the enzymolysis ovalbumin solution, wherein the rotation speed of the centrifuge is 4000r/min, and the centrifugation time is 6 min;
step six: and (3) post-treatment: sterilizing, concentrating and drying the enzymolysis ovalbumin liquid to obtain the enzymolysis ovalbumin.
Example 2:
referring to fig. 1, the present invention provides a technical solution: the preparation method of the enzymolysis ovalbumin comprises the following raw materials in parts by weight: 0.36 part of ornithine, 3.75 parts of lysine, 1.3 parts of histidine, 3.87 parts of arginine, 2.30 parts of threonine, 3.75 parts of serine, 4.82 parts of glutamic acid, 1.79 parts of proline, 1.64 parts of glycine, 3.51 parts of alanine, 3.70 parts of cystine, 5.67 parts of valine, 6.18 parts of leucine, 2.51 parts of isoleucine, 0.92 part of tyrosine, 4.79 parts of phenylalanine, 1.73 parts of aspartic acid, 6.40 parts of methionine and the balance of water; the preparation method of the enzymolysis ovalbumin comprises the following specific steps:
the method comprises the following steps: obtaining egg white: soaking the collected eggs until impurities on the outer surfaces of the eggs are separated from the eggs, fishing out the eggs, washing the eggs with clear water, sterilizing the eggs with sterile water, crushing the eggs at 5 ℃ under an aseptic condition, separating egg white from egg yolk, keeping the egg white, treating the egg white by a pasteurization method, and cooling to 4 ℃ to obtain the egg white, wherein the pasteurization method is carried out at 65 ℃, the heat preservation time is 0.35h, and the cooling time is 2.5 min;
step two: preparing an immobilized enzyme: mixing papain and trypsin to form mixed enzyme, preparing a mixed enzyme carrier, and carrying out covalent connection on the mixed enzyme carrier and the mixed enzyme to obtain the immobilized enzyme, wherein the specific operation of preparing the mixed enzyme carrier is as follows:
s1, sequentially putting the multi-walled carbon nanotube, the nano titanium dioxide and the ferroferric oxide powder into grinding equipment, grinding and stirring the raw materials to fully mix the raw materials to obtain mixed powder, drying the mixed powder, dispersing the mixed powder into a polyethylene glycol aqueous solution, dropwise adding vinyltriethoxysilane, stirring by using stirring equipment, finally adding a tetramethyl orthosilicate solution for mixing, and obtaining a mixed solution after mixing, wherein the mixed powder is dispersed by an ultrasonic dispersion method for 0.75h at a dispersion temperature of 50 ℃;
s2, mixing liquid paraffin and trichloromethane, adding an emulsifier after mixing, continuously stirring, keeping the temperature at 60 ℃ all the time in the process, adding the mixed solution while stirring the emulsifier, stirring for 0.5 hour again, cooling to room temperature, and centrifuging in a centrifuge to form a supernatant and a pre-carrier;
s3, removing the supernatant in the step S2, reserving the pre-carrier, cleaning the pre-carrier by using ethyl acetate, and finally washing by using clear water to obtain a mixed enzyme carrier;
step three: enzymolysis: weighing the egg white in the first step, adding water, stirring into emulsion, adjusting the pH value and the temperature to a proper value to form a reaction substrate, putting the immobilized enzyme in the second step into the reaction substrate for hydrolysis reaction for 7 hours, and obtaining an enzymolysis solution after the reaction is finished, wherein the proper pH value and the proper temperature are respectively 7 and 37 ℃;
step four: and (3) recovering the immobilized enzyme: electrifying the electromagnet, putting the electromagnet into the enzymolysis solution, stirring to enable the electromagnet to adsorb the immobilized enzyme in the enzymolysis solution, taking out the electromagnet, powering off the electromagnet to enable the immobilized enzyme adsorbed on the electromagnet to fall off, repeating the process until no immobilized enzyme is adsorbed, and completing the recovery of the immobilized enzyme, wherein the magnetic field intensity of the electromagnet is 0.5T;
step five: centrifugal screening: heating the enzymolysis solution obtained in the fourth step to 92 ℃, preserving heat for 25min, cooling to room temperature to passivate mixed enzyme remaining in the enzymolysis solution, placing the enzymolysis solution into a centrifuge for centrifugal separation to form supernatant and residue, removing the residue and retaining the supernatant, namely the enzymolysis ovalbumin solution, wherein the rotation speed of the centrifuge is 4300r/min, and the centrifugation time is 7 min;
step six: and (3) post-treatment: sterilizing, concentrating and drying the enzymolysis ovalbumin liquid to obtain the enzymolysis ovalbumin.
Example 3:
referring to fig. 1, the present invention provides a technical solution: the preparation method of the enzymolysis ovalbumin comprises the following raw materials in parts by weight: 0.51 part of ornithine, 6.00 parts of lysine, 1.80 parts of histidine, 5.50 parts of arginine, 3.80 parts of threonine, 6.00 parts of serine, 8.24 parts of glutamic acid, 2.58 parts of proline, 2.98 parts of glycine, 5.17 parts of alanine, 5.20 parts of cystine, 8.03 parts of valine, 8.03 parts of leucine, 3.86 parts of isoleucine, 1.44 parts of tyrosine, 7.33 parts of phenylalanine, 2.44 parts of aspartic acid, 8.40 parts of methionine and the balance of water; the preparation method of the enzymolysis ovalbumin comprises the following specific steps:
the method comprises the following steps: obtaining egg white: soaking the collected eggs until impurities on the outer surfaces of the eggs are separated from the eggs, fishing out the eggs, washing the eggs with clear water, sterilizing the eggs with sterile water, crushing the eggs at 6 ℃ under an aseptic condition, separating egg white from egg yolk, keeping the egg white, treating the egg white by a pasteurization method, and cooling to 4 ℃ to obtain the egg white, wherein the pasteurization method is carried out at the temperature of 70 ℃, the heat preservation time is 0.5h, and the cooling time is 3 min;
step two: preparing an immobilized enzyme: mixing papain and trypsin to form mixed enzyme, preparing a mixed enzyme carrier, and carrying out covalent connection on the mixed enzyme carrier and the mixed enzyme to obtain the immobilized enzyme, wherein the specific operation of preparing the mixed enzyme carrier is as follows:
s1, sequentially putting the multi-walled carbon nanotube, the nano titanium dioxide and the ferroferric oxide powder into grinding equipment, grinding and stirring the raw materials to fully mix the raw materials to obtain mixed powder, drying the mixed powder, dispersing the mixed powder into a polyethylene glycol aqueous solution, dropwise adding vinyltriethoxysilane, stirring by using stirring equipment, finally adding a tetramethyl orthosilicate solution for mixing, and obtaining a mixed solution after mixing, wherein the mixed powder is dispersed by an ultrasonic dispersion method for 1h at a dispersion temperature of 50 ℃;
s2, mixing liquid paraffin and trichloromethane, adding an emulsifier after mixing, continuously stirring, keeping the temperature at 60 ℃ all the time in the process, adding the mixed solution while stirring the emulsifier, stirring for 0.5 hour again, cooling to room temperature, and centrifuging in a centrifuge to form a supernatant and a pre-carrier;
s3, removing the supernatant in the step S2, reserving the pre-carrier, cleaning the pre-carrier by using ethyl acetate, and finally washing by using clear water to obtain a mixed enzyme carrier;
step three: enzymolysis: weighing the egg white in the first step, adding water, stirring into emulsion, adjusting the pH value and temperature to form a reaction substrate, putting the immobilized enzyme in the second step into the reaction substrate for hydrolysis reaction for 8 hours, and obtaining an enzymolysis solution after the reaction is finished, wherein the pH value and the temperature are respectively 8 and 40 ℃;
step four: and (3) recovering the immobilized enzyme: electrifying the electromagnet, putting the electromagnet into the enzymolysis solution, stirring to enable the electromagnet to adsorb the immobilized enzyme in the enzymolysis solution, taking out the electromagnet, powering off the electromagnet to enable the immobilized enzyme adsorbed on the electromagnet to fall off, repeating the process until no immobilized enzyme is adsorbed, and completing the recovery of the immobilized enzyme, wherein the magnetic field intensity of the electromagnet is 0.6T;
step five: centrifugal screening: heating the enzymolysis solution obtained in the fourth step to 95 ℃, preserving heat for 30min, cooling to room temperature to passivate mixed enzyme remaining in the enzymolysis solution, placing the enzymolysis solution into a centrifuge for centrifugal separation to form supernatant and residue, removing the residue and retaining the supernatant, namely the enzymolysis ovalbumin solution, wherein the rotation speed of the centrifuge is 4500r/min, and the centrifugation time is 8 min;
step six: and (3) post-treatment: sterilizing, concentrating and drying the enzymolysis ovalbumin liquid to obtain the enzymolysis ovalbumin.
Comparative example 1:
referring to fig. 1, the present invention provides a technical solution: a preparation method of enzymolysis ovalbumin does not carry out the processes of preparing immobilized enzyme and recovering the immobilized enzyme, and directly adds mixed enzyme into a reaction substrate for enzymolysis, and the other schemes are the same as the embodiment 1.
The comparison shows that: the enzymolysis ovalbumin prepared in the examples 1-3 and the comparative example 1 has similar amount, but the enzymolysis process of the examples 1-3 is more controllable, meanwhile, the mixed enzyme in the comparative example 1 is inactivated or passivated in subsequent processing, only one processing can be completed, the immobilized enzyme can be recovered, and the mixed enzyme can be repeatedly used, so that the input of the preparation cost is reduced, and the ferroferric oxide powder is added into the mixed enzyme carrier, so that the mixed enzyme carrier contains iron element, the electromagnet can adsorb the immobilized enzyme, the recovery of the immobilized enzyme is simplified, the time and the labor are saved, the production efficiency of the enzymolysis ovalbumin is also guaranteed, and the operation is completed.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. The preparation method of the enzymolysis ovalbumin is characterized in that the enzymolysis ovalbumin is composed of the following raw materials in parts by weight: 0.21-0.51 part of ornithine, 1.50-6.00 parts of lysine, 0.80-1.80 parts of histidine, 2.25-5.50 parts of arginine, 0.80-3.80 parts of threonine, 1.50-6.00 parts of serine, 1.41-8.24 parts of glutamic acid, 1.00-2.58 parts of proline, 0.30-2.98 parts of glycine, 1.86-5.17 parts of alanine, 2.20-5.20 parts of cystine, 3.32-8.03 parts of valine, 4.34-8.03 parts of leucine, 1.17-3.86 parts of isoleucine, 0.41-1.44 parts of tyrosine, 2.25-7.33 parts of phenylalanine, 1.03-2.44 parts of aspartic acid and 2.49-8.40 parts of methionine, and the balance of water; the preparation method of the enzymolysis ovalbumin comprises the following specific steps:
the method comprises the following steps: obtaining egg white: soaking the collected eggs until impurities on the outer surfaces of the eggs are separated from the eggs, then fishing out the eggs, washing the eggs with clear water, sterilizing with sterile water, crushing the eggs under the aseptic condition of 4-6 ℃, then separating egg white from yolk, keeping the egg white, finally treating the egg white by a pasteurization method, and cooling to 4 ℃ to obtain egg white;
step two: preparing an immobilized enzyme: mixing papain and trypsin to form mixed enzyme, preparing a mixed enzyme carrier, and carrying out covalent connection on the mixed enzyme carrier and the mixed enzyme to obtain an immobilized enzyme;
step three: enzymolysis: weighing the egg white in the first step, adding water, stirring into emulsion, adjusting the pH value and temperature to form a reaction substrate, putting the immobilized enzyme in the second step into the reaction substrate for hydrolysis reaction for 6-8h, and obtaining an enzymolysis solution after the reaction is finished;
step four: and (3) recovering the immobilized enzyme: electrifying the electromagnet, putting the electromagnet into the enzymolysis solution for stirring, enabling the electromagnet to adsorb the immobilized enzyme in the enzymolysis solution, taking out the electromagnet, powering off the electromagnet, enabling the immobilized enzyme adsorbed on the electromagnet to fall off, repeating the process until no immobilized enzyme is adsorbed, and completing the recovery of the immobilized enzyme;
step five: centrifugal screening: heating the enzymolysis solution obtained in the fourth step to 90-95 ℃, preserving heat for 20-30min, cooling to room temperature to passivate mixed enzyme remaining in the enzymolysis solution, placing the enzymolysis solution into a centrifuge for centrifugal separation to form supernatant and residues, removing the residues, and retaining the supernatant to obtain enzymolysis ovalbumin liquid;
step six: and (3) post-treatment: sterilizing, concentrating and drying the enzymolysis ovalbumin liquid to obtain the enzymolysis ovalbumin.
2. The method for preparing enzymatically hydrolyzed ovalbumin according to claim 1, wherein the pasteurization in the first step is carried out at a temperature of 60 to 70 ℃, the holding time is 0.25 to 0.5h, and the cooling time is 2 to 3 min.
3. The method for preparing enzymolysis ovalbumin according to claim 1, wherein the specific operation of preparing the mixed enzyme carrier in the second step is as follows:
s1, sequentially putting the multi-walled carbon nanotube, the nano titanium dioxide and the ferroferric oxide powder into grinding equipment, grinding and stirring the raw materials to fully mix the raw materials to obtain mixed powder, drying the mixed powder, dispersing the mixed powder into a polyethylene glycol aqueous solution, dropwise adding vinyltriethoxysilane, stirring the mixture by using stirring equipment, finally adding a tetramethyl orthosilicate solution for mixing, and obtaining a mixed solution after the mixing is finished;
s2, mixing liquid paraffin and trichloromethane, adding an emulsifier after mixing, continuously stirring, keeping the temperature at 60 ℃ all the time in the process, adding the mixed solution while stirring the emulsifier, stirring for 0.5 hour again, cooling to room temperature, and centrifuging in a centrifuge to form a supernatant and a pre-carrier;
s3, removing the supernatant in the step S2, reserving the pre-carrier, cleaning the pre-carrier by ethyl acetate, and finally washing by clear water to obtain the mixed enzyme carrier.
4. The method for preparing enzymatically hydrolyzed ovalbumin according to claim 3, wherein the dispersion of the mixed powder in step S1 is carried out by ultrasonic dispersion for 0.5-1h at 50 ℃.
5. The method for preparing enzymatically hydrolyzed ovalbumin according to claim 1, wherein the appropriate pH and temperature in step three are 6-8 and 36-40 ℃ respectively.
6. The method for preparing enzymolysis ovalbumin according to claim 1, wherein the magnetic field intensity of the electromagnet in the fourth step is 0.4-0.6T.
7. The method as claimed in claim 1, wherein the rotation speed of the centrifuge in step five is 4000-.
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| CN1175360A (en) * | 1996-09-01 | 1998-03-11 | 魏剑鸣 | Egg albumin zymolysis matter and preparing process thereof |
| CN103495376A (en) * | 2013-10-08 | 2014-01-08 | 江南大学 | Preparation and phosphoeptide enrichment methods of titanium dioxide/ferroferric oxide nanoparticles |
| CN106075385A (en) * | 2016-08-17 | 2016-11-09 | 贵州佰世合意股份有限公司 | A kind of polypeptide oral liquor and preparation method thereof |
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