CN114503917A - Method for promoting tissue culture rooting of plukenetia volubilis linneo - Google Patents
Method for promoting tissue culture rooting of plukenetia volubilis linneo Download PDFInfo
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- CN114503917A CN114503917A CN202210276952.0A CN202210276952A CN114503917A CN 114503917 A CN114503917 A CN 114503917A CN 202210276952 A CN202210276952 A CN 202210276952A CN 114503917 A CN114503917 A CN 114503917A
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- 241001300674 Plukenetia volubilis Species 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 14
- 230000001737 promoting effect Effects 0.000 title claims abstract description 13
- 229910052761 rare earth metal Inorganic materials 0.000 claims abstract description 27
- -1 auxin rare earth Chemical class 0.000 claims abstract description 26
- 229930192334 Auxin Natural products 0.000 claims abstract description 17
- 239000002363 auxin Substances 0.000 claims abstract description 17
- 239000012883 rooting culture medium Substances 0.000 claims abstract description 13
- JTEDVYBZBROSJT-UHFFFAOYSA-N 1H-Indole-3-butanoic acid Natural products C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 55
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 239000013078 crystal Substances 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000004090 dissolution Methods 0.000 claims description 4
- 229910052684 Cerium Inorganic materials 0.000 claims description 3
- 239000002274 desiccant Substances 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- VBDSTGDTZFQGNC-UHFFFAOYSA-M sodium;4-(1h-indol-3-yl)butanoate Chemical compound [Na+].C1=CC=C2C(CCCC(=O)[O-])=CNC2=C1 VBDSTGDTZFQGNC-UHFFFAOYSA-M 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 238000001308 synthesis method Methods 0.000 claims description 2
- VGKONPUVOVVNSU-UHFFFAOYSA-N naphthalen-1-yl acetate Chemical compound C1=CC=C2C(OC(=O)C)=CC=CC2=C1 VGKONPUVOVVNSU-UHFFFAOYSA-N 0.000 claims 3
- 229910052772 Samarium Inorganic materials 0.000 claims 1
- 229910052746 lanthanum Inorganic materials 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 5
- 208000024172 Cardiovascular disease Diseases 0.000 abstract description 2
- 241000221017 Euphorbiaceae Species 0.000 abstract description 2
- 239000008280 blood Substances 0.000 abstract description 2
- 210000004369 blood Anatomy 0.000 abstract description 2
- 239000002537 cosmetic Substances 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 235000021315 omega 9 monounsaturated fatty acids Nutrition 0.000 abstract description 2
- 235000020660 omega-3 fatty acid Nutrition 0.000 abstract description 2
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 241001553178 Arachis glabrata Species 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 abstract 1
- 235000019197 fats Nutrition 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 235000020232 peanut Nutrition 0.000 abstract 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 7
- GOLXRNDWAUTYKT-UHFFFAOYSA-N 3-(1H-indol-3-yl)propanoic acid Chemical compound C1=CC=C2C(CCC(=O)O)=CNC2=C1 GOLXRNDWAUTYKT-UHFFFAOYSA-N 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 3
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 229910052688 Gadolinium Inorganic materials 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- 229910052771 Terbium Inorganic materials 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 238000004455 differential thermal analysis Methods 0.000 description 2
- 230000009700 hormesis effect Effects 0.000 description 2
- 239000003290 indole 3-propionic acid Substances 0.000 description 2
- 239000003617 indole-3-acetic acid Substances 0.000 description 2
- 238000002329 infrared spectrum Methods 0.000 description 2
- 238000001819 mass spectrum Methods 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 238000002186 photoelectron spectrum Methods 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 238000002411 thermogravimetry Methods 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- 238000002211 ultraviolet spectrum Methods 0.000 description 2
- 241000554155 Andes Species 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- GWXLDORMOJMVQZ-UHFFFAOYSA-N cerium Chemical compound [Ce] GWXLDORMOJMVQZ-UHFFFAOYSA-N 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- UIWYJDYFSGRHKR-UHFFFAOYSA-N gadolinium atom Chemical compound [Gd] UIWYJDYFSGRHKR-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- GZCRRIHWUXGPOV-UHFFFAOYSA-N terbium atom Chemical compound [Tb] GZCRRIHWUXGPOV-UHFFFAOYSA-N 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for promoting tissue culture rooting of plukenetia volubilis, which is also called as plukenetia volubilis, Indian fruits, Indian peanuts and the like, is a perennial woody vine oil plant of the euphorbiaceae, and the plukenetia volubilis oil squeezed from the seeds of the plukenetia volubilis is rich in omega-3, omega-6, omega-9 and other polyunsaturated fatty acids, can be used in the fields of food, medicine, cosmetics and the like, and has remarkable effects on regulating blood fat, preventing cardiovascular diseases, maintaining skin and the like. Tissue culture is one of the main propagation modes for obtaining a large number of high-quality plukenetia volubilis seedlings in a short period, but the tissue culture seedlings have the problems of slow rooting and difficult rooting. Therefore, the method can obviously promote the tissue culture rooting of the plukenetia volubilis linneo by adding 30-50 mu mol/L auxin rare earth complex into the rooting culture medium, has long root system, more rooting number and high rooting rate, solves the problem of difficult tissue culture rooting of the plukenetia volubilis linneo, and promotes the industrial production of plukenetia volubilis linneo seedlings.
Description
Technical Field
The invention relates to a plant tissue culture method, in particular to a method for promoting tissue culture rooting of plukenetia volubilis linneo.
Background
Plukenetia volubilis L, also known as Plukenetia volubilis L, Plukenetia volubilis, and Arachis hypogaea, is a perennial woody vine oil plant of the family Euphorbiaceae, originally produced in tropical rainforest of Andes mountain area of south America, and the Plukenetia volubilis oil extracted from its seeds is rich in polyunsaturated fatty acids such as omega-3, omega-6, omega-9, etc., is considered to be a high-end vegetable oil, can be used in the fields of food, medicine, cosmetics, etc., and has remarkable effects on regulating blood lipid, preventing cardiovascular diseases, caring skin, etc. Tissue culture is one of the main propagation modes for obtaining a large number of high-quality plukenetia volubilis seedlings in a short period, but the problems of slow rooting and difficult rooting exist in the tissue culture process.
Disclosure of Invention
The invention aims to provide a method for promoting tissue culture rooting of plukenetia volubilis.
The invention discloses a method for promoting tissue culture rooting of plukenetia volubilis linneo, which comprises the following steps:
(1) cutting and inoculating adventitious buds with the length of about 3-5 cm obtained by tissue culture into a rooting culture medium for induced rooting culture, wherein the rooting culture medium is 1/2MS + 1.0-2.0 mg/L GGR + 30-50 mu mol/L auxin rare earth complex + 15-30 g/L sucrose + 3.5-6.0 g/L agar, and the pH value is 5.4-5.8;
(2) after inoculation, the seeds are cultured in the dark for 3 days at 25 ℃, and then cultured under the conditions of 12 hours of illumination each day, 3000lx of illumination intensity and 25 ℃ of culture temperature until the roots are rooted.
The auxin rare earth complex in the step (1) is an indole-3-butyric acid rare earth complex RE (IBA)3·2H2And O. Specifically, the auxin rare earth complex is one of an indole-3-terbium butyrate complex, an indole-3-cerium butyrate complex and an indole-3-gadolinium butyrate complex.
The auxin rare earth complex in the step (1) is prepared from rare earth chloride (RECl)3·6H2O) and indole-3-butyric acid sodium salt (C)12H12NO2Na) is adopted. The synthesis method comprises the following steps: RE is first introduced2O3Dissolving in a slight excess of 1:1 hydrochloric acid, addingAdding a proper amount of H2O2Accelerating dissolution, and heating to make residual H2O2Decomposing completely, concentrating under reduced pressure, vacuum filtering, recrystallizing for 3 times to obtain crystal P2O5Drying in a drying agent until the weight is constant, and measuring RE by EDTA titration3+Determination of Cl by silver method-And determining the crystal water by thermogravimetry to verify that the crystal composition is RECl3·6H2O; then metering RECl according to the chemical formula3·6H2O IBA ═ 1:3, weighing, dissolving IBA in 95% ethanol, and dropwise adding 3mol/L NaOH solution to generate indole-3-sodium butyrate solution; dissolving rare earth chloride in 95% ethanol, dropping in indole-3-sodium butyrate solution to obtain precipitate, filtering, washing with distilled water until no Cl is formed-Vacuum drying to obtain solid complex; the composition of the auxin rare earth complex is RE (IBA) proved by element analysis, mass spectrum, ultraviolet spectrum, infrared spectrum, X photoelectron spectrum, thermogravimetric-differential thermal analysis and the like3·2H2O, where RE is Tb3+、Ce3 +、Gd3+(ii) a IBA is indole-3-butyric acid (C)12H13NO2)。
Compared with the prior art, the invention has the beneficial effects that:
1. the auxin has the characteristic of promoting the growth of plants and can also promote the rooting of the plants, and the biological activity of the auxin relates to the bonding effect of metal ions in plants. The auxin widely used in the rooting culture medium at present mainly comprises indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and the like. Rare earths have a Hormesis effect on plants, similar to the action of hormones. According to the invention, by synthesizing the auxin rare earth complex, adding the auxin rare earth complex into a rooting culture medium, and utilizing the biological effect generated by the cooperation of the effect of promoting plant rooting by using indole-3-butyric acid and the Hormesis effect of the rare earth element, the tissue culture rooting of the plukenetia volubilis is obviously promoted, the root system is long, the rooting number is large, the rooting rate is high, the operation is simple and convenient, the effect is good, the problem that the plukenetia volubilis is difficult to root during tissue culture is solved, and the industrial development of plukenetia volubilis seedlings is promoted.
Detailed Description
The following examples are further illustrative of the present invention and are not intended to be limiting thereof.
EXAMPLE Synthesis of a auxin rare earth Complex
(1) Preparing rare earth chloride: RE is mixed with2O3(RE＝Tb3+、Ce3+、Gd3+) Dissolving in 1:1 hydrochloric acid in slight excess, adding appropriate amount of H2O2Accelerating dissolution, and heating to make residual H2O2Decomposing completely, concentrating under reduced pressure, vacuum filtering, recrystallizing for 3 times to obtain crystal P2O5Drying in a drying agent until the weight is constant, and measuring RE by EDTA titration3+Determination of Cl by silver method-And determining the crystal water by thermogravimetry to verify that the crystal composition is RECl3·6H2O。
(2) Preparation of auxin rare earth complex: metering RECl by formula3·6H2IBA ═ 1:3, weighing, dissolving IBA in 95% ethanol, and dropwise adding 3mol/L NaOH solution to generate indole-3-sodium butyrate solution; dissolving rare earth chloride in 95% ethanol, dropping in indole-3-sodium butyrate solution to obtain precipitate, filtering, washing with distilled water until no Cl is formed-And vacuum drying to obtain the solid complex.
(3) Characterization of the auxin rare earth complex: the composition of the auxin rare earth complex is RE (IBA) proved by element analysis, mass spectrum, ultraviolet spectrum, infrared spectrum, X photoelectron spectrum, thermogravimetric-differential thermal analysis and the like3·2H2O, where RE is Tb3+、Ce3+、Gd3+(ii) a IBA is indole-3-butyric acid (C)12H13NO2)。
Example diindole-3-butyric acid terbium complex [ Tb (IBA)3·2H2O]Influence on rooting of tissue culture seedlings of plukenetia volubilis
(1) Cutting and inoculating the adventitious bud with the length of about 3-5 cm obtained by tissue culture into a rooting culture medium for induced rooting culture, wherein the rooting culture medium is 1/2MS + 1.0-2.0 mg/L GGR + 30-50 mu mol/L Tb (IBA)3·2H215-30 g/L of O + sucrose + 3.5-6.0 g/L of agar, and the pH value is 5.4-5.8.
(2) After inoculation, the seeds are cultured for 3 days in the dark at 25 ℃, and then cultured under the conditions of 12 hours of illumination each day, 3000lx of illumination intensity and 25 ℃ of culture temperature. After 7 days of culture, the rooting is started, and the rooting rate reaches more than 98.5 percent; after 15 days of culture, the root length is 2.44-2.86 cm, and the number of roots reaches 3.84-4.21 per plant.
Tb(IBA)3·2H2The concentration of O may be 30. mu. mol/L, 35. mu. mol/L, 40. mu. mol/L, 45. mu. mol/L, 50. mu. mol/L.
Example Triindole-3-butyric acid cerium Complex [ Ce (IBA)3·2H2O]Influence on rooting of tissue culture seedlings of plukenetia volubilis
(1) Cutting and inoculating the adventitious bud with the length of about 3-5 cm obtained by tissue culture into a rooting culture medium for induced rooting culture, wherein the rooting culture medium is 1/2MS + 1.0-2.0 mg/L GGR + 30-50 mu mol/L Ce (IBA)3·2H215-30 g/L of O + sucrose + 3.5-6.0 g/L of agar, and the pH value is 5.4-5.8.
(2) After inoculation, the seeds are cultured for 3 days in the dark at 25 ℃, and then cultured under the conditions of 12 hours of illumination each day, 3000lx of illumination intensity and 25 ℃ of culture temperature. After 8 days of culture, the rooting is started, and the rooting rate reaches 100%; after culturing for 15 days, the root length is 2.04-2.36 cm, and the root number reaches 4.14-4.52 per plant.
Ce(IBA)3·2H2The concentration of O may be 30. mu. mol/L, 35. mu. mol/L, 40. mu. mol/L, 45. mu. mol/L, 50. mu. mol/L.
Example Tetraindole-3-butyric acid gadolinium complex [ Gd (IBA)3·2H2O]Influence on rooting of tissue culture seedlings of plukenetia volubilis
(1) Cutting and inoculating the adventitious bud with the length of about 3-5 cm obtained by tissue culture into a rooting culture medium for induced rooting culture, wherein the rooting culture medium is 1/2MS + 1.0-2.0 mg/L GGR + 30-50 mu mol/L Gd (IBA)3·2H215-30 g/L of O + sucrose + 3.5-6.0 g/L of agar, and the pH value is 5.4-5.8;
(2) after inoculation, the seeds are cultured for 3 days in the dark at 25 ℃, and then cultured under the conditions of 12 hours of illumination each day, 3000lx of illumination intensity and 25 ℃ of culture temperature. After culturing for 9 days, the rooting is started, and the rooting rate reaches 97.5%; after culturing for 15 days, the root length is 2.14-2.46 cm, and the number of roots reaches 3.84-4.16 per plant.
Gd(IBA)3·2H2The concentration of O may be 30. mu. mol/L, 35. mu. mol/L, 40. mu. mol/L, 45. mu. mol/L, 50. mu. mol/L.
Claims (5)
1. A method for promoting tissue culture rooting of plukenetia volubilis linneo is characterized by comprising the following steps:
(1) cutting and inoculating adventitious buds with the length of about 3-5 cm obtained by tissue culture into a rooting culture medium for induced rooting culture, wherein the rooting culture medium is 1/2MS + 1.0-2.0 mg/L GGR + 30-50 mu mol/L auxin rare earth complex + 15-30 g/L sucrose + 3.5-6.0 g/L agar, and the pH value is 5.4-5.8;
(2) after inoculation, the seeds are cultured in the dark for 3 days at 25 ℃, and then cultured under the conditions of 12 hours of illumination each day, 3000lx of illumination intensity and 25 ℃ of culture temperature until the roots are rooted.
2. The method for promoting tissue culture rooting of plukenetia volubilis linneo according to claim 1, wherein the auxin rare earth complex is an indole-3-butyric acid rare earth complex.
3. The method for promoting tissue culture rooting of plukenetia volubilis linneo according to claim 2, wherein the indole-3-butyric acid rare earth complex is one of an alpha-lanthanum naphthylacetate complex, an alpha-cerium naphthylacetate complex and an alpha-samarium naphthylacetate complex.
4. The method for promoting tissue culture rooting of plukenetia volubilis linneo according to claim 3, wherein the indole-3-butyric acid rare earth complex is synthesized from rare earth chloride and indole-3-butyric acid sodium salt.
5. The method for promoting tissue culture rooting of plukenetia volubilis linneo according to claim 4, wherein the synthesis method of the indole-3-butyric acid rare earth complex comprises the following steps: RE is mixed with2O3Dissolving in 1:1 hydrochloric acid in slight excess, adding H2O2Accelerating dissolution, heating to make residual H after complete dissolution2O2Decomposing completely, concentrating under reduced pressure, vacuum filtering, recrystallizing for 3 times to obtain crystal P2O5Drying in a drying agent until the weight is constant to obtain the rare earth chloride RECl3·6H2O; then metering RECl according to the formula3·6H2Weighing IBA (1: 3), and dissolving IBA in 0.015mol/L NaOH solution to generate indole-3-butyric acid sodium salt; dissolving rare earth chloride with 95% ethanol, dropwise adding indole-3-sodium butyrate, stirring, standing to obtain precipitate, filtering, washing with distilled water until no Cl is formed-Vacuum drying to obtain solid complex RE (IBA)3·2H2O。
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798684A (en) * | 2015-04-25 | 2015-07-29 | 玉林师范学院 | Tissue culture rapid propagation method for plukenetia volubilis L. |
| CN106342689A (en) * | 2016-09-06 | 2017-01-25 | 海南大学 | Method for quickly propagating plukenetia volubilis linneo |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798684A (en) * | 2015-04-25 | 2015-07-29 | 玉林师范学院 | Tissue culture rapid propagation method for plukenetia volubilis L. |
| CN106342689A (en) * | 2016-09-06 | 2017-01-25 | 海南大学 | Method for quickly propagating plukenetia volubilis linneo |
Non-Patent Citations (2)
| Title |
|---|
| 冯邦朝等: "大戟科主要木本油料植物组织培养快繁技术研究进展", 《南方农业学报》 * |
| 王则民: "稀土植物生长素类固体配合物的研究进展", 《稀土》 * |
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