CN114502742A - 用于核酸测序及相关应用的方法和试剂 - Google Patents

用于核酸测序及相关应用的方法和试剂 Download PDF

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Publication number
CN114502742A
CN114502742A CN202080055766.3A CN202080055766A CN114502742A CN 114502742 A CN114502742 A CN 114502742A CN 202080055766 A CN202080055766 A CN 202080055766A CN 114502742 A CN114502742 A CN 114502742A
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nucleic acid
strand
amplicons
double
stranded
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Chinese (zh)
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J·J·索尔克
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Twinstrand Biosciences Inc
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Twinstrand Biosciences Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202080055766.3A 2019-08-01 2020-08-01 用于核酸测序及相关应用的方法和试剂 Pending CN114502742A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962881936P 2019-08-01 2019-08-01
US62/881,936 2019-08-01
PCT/US2020/044673 WO2021022237A1 (en) 2019-08-01 2020-08-01 Methods and reagents for nucleic acid sequencing and associated applications

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CN114502742A true CN114502742A (zh) 2022-05-13

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CN202080055766.3A Pending CN114502742A (zh) 2019-08-01 2020-08-01 用于核酸测序及相关应用的方法和试剂

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US (1) US20220220543A1 (https=)
EP (1) EP4007818A4 (https=)
JP (2) JP2022543778A (https=)
CN (1) CN114502742A (https=)
AU (1) AU2020321991A1 (https=)
CA (1) CA3146435A1 (https=)
IL (1) IL290274A (https=)
WO (1) WO2021022237A1 (https=)

Families Citing this family (12)

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Publication number Priority date Publication date Assignee Title
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
SG11201906428SA (en) 2017-01-18 2019-08-27 Illumina Inc Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
AU2018261332B2 (en) 2017-05-01 2024-12-05 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
EP3622089B1 (en) 2017-05-08 2024-07-17 Illumina, Inc. Method for sequencing using universal short adapters for indexing of polynucleotide samples
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
WO2021178893A2 (en) 2020-03-06 2021-09-10 Singular Genomics Systems, Inc. Linked paired strand sequencing
CN115109845B (zh) * 2021-03-18 2025-07-25 武汉大学 Rna中次黄嘌呤的高通量测序检测方法
AU2023236924A1 (en) * 2022-03-15 2024-10-24 Illumina, Inc. Paired-end sequencing
US12091715B2 (en) 2022-04-21 2024-09-17 Paragon Genomics, Inc. Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing
US11680293B1 (en) * 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
GB202209189D0 (en) * 2022-06-22 2022-08-10 Broken String Biosciences Ltd Methods and compositions for nucleic acid sequencing
EP4728092A1 (en) * 2023-06-14 2026-04-22 Illumina, Inc. Determination of modified cytosines

Citations (3)

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Publication number Priority date Publication date Assignee Title
WO2018023068A1 (en) * 2016-07-29 2018-02-01 New England Biolabs, Inc. Methods and compositions for preventing concatemerization during template- switching
WO2018175997A1 (en) * 2017-03-23 2018-09-27 University Of Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
CN109072294A (zh) * 2015-12-08 2018-12-21 特温斯特兰德生物科学有限公司 用于双重测序的改良衔接子、方法和组合物

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WO2012012037A1 (en) * 2010-07-19 2012-01-26 New England Biolabs, Inc. Oligonucleotide adaptors: compositions and methods of use
WO2012061832A1 (en) * 2010-11-05 2012-05-10 Illumina, Inc. Linking sequence reads using paired code tags
WO2013123442A1 (en) * 2012-02-17 2013-08-22 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations
PT2828218T (pt) * 2012-03-20 2020-11-11 Univ Washington Through Its Center For Commercialization Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus
EP2847353B1 (en) * 2012-05-10 2022-01-19 The General Hospital Corporation Methods for determining a nucleotide sequence
ES2660989T3 (es) * 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
US10435685B2 (en) * 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
US10465241B2 (en) * 2015-06-15 2019-11-05 The Board Of Trustees Of The Leleand Stanford Junior University High resolution STR analysis using next generation sequencing
WO2018183942A1 (en) * 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109072294A (zh) * 2015-12-08 2018-12-21 特温斯特兰德生物科学有限公司 用于双重测序的改良衔接子、方法和组合物
WO2018023068A1 (en) * 2016-07-29 2018-02-01 New England Biolabs, Inc. Methods and compositions for preventing concatemerization during template- switching
WO2018175997A1 (en) * 2017-03-23 2018-09-27 University Of Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing

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Publication number Publication date
WO2021022237A1 (en) 2021-02-04
US20220220543A1 (en) 2022-07-14
IL290274A (en) 2022-04-01
CA3146435A1 (en) 2021-02-04
JP2026035740A (ja) 2026-03-04
EP4007818A4 (en) 2023-09-20
EP4007818A1 (en) 2022-06-08
AU2020321991A1 (en) 2022-03-03
JP2022543778A (ja) 2022-10-14

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