CN114488548A - Method and system for generating high-operability optical tweezers - Google Patents

Method and system for generating high-operability optical tweezers Download PDF

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CN114488548A
CN114488548A CN202210400860.9A CN202210400860A CN114488548A CN 114488548 A CN114488548 A CN 114488548A CN 202210400860 A CN202210400860 A CN 202210400860A CN 114488548 A CN114488548 A CN 114488548A
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optical tweezers
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axis
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CN114488548B (en
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邓冬梅
石景溢
梁泽弘
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South China Normal University
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    • G02B27/00Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0938Using specific optical elements
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0938Using specific optical elements
    • G02B27/0944Diffractive optical elements, e.g. gratings, holograms
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Abstract

The invention discloses a method and a system for generating high-operability optical tweezers, wherein the method comprises the steps of simulating plane waves by a computer to interfere with a random electric field with double off-axis Laguerre Gaussian superimposed beams and distorted Gaussian Sieger model information to obtain 100 different phase holograms, loading the amplitude and phase information of the beams on interference fringes, transmitting the obtained phase holograms to a spatial light modulator, and refreshing at the frequency of 60 HZ; starting a helium-neon laser to emit a Gaussian beam to pass through a beam expander; after light irradiates the spatial light modulator, a partially coherent dual off-axis Laguerre Gaussian superposition distorted light beam can be obtained after the light is transmitted by a 4f system; and transmitting the light beam to an optical tweezers system after passing through a time delay system. Thus obtaining the optical tweezers with high operability in the optical tweezers system. The invention can realize fixed-angle rotation of the optical tweezers, and can realize higher-precision control of particles in the aspect of capturing and manipulating the particles.

Description

Method and system for generating high-operability optical tweezers
Technical Field
The invention relates to the technical field of optics, in particular to a method and a system for generating high-operability optical tweezers.
Background
Laser beams are known for high spatial coherence and are often considered to be fully coherent beams. However, there are significant advantages in many areas when the beam is at low spatial coherence. The distorted phase, which is a phase specific to the partially coherent light beam, can cause the light beam to rotate during propagation due to internal asymmetry, and has potential application value in optical manipulation. The laguerre gaussian beam is of great interest for research due to its optical vortex and stable optical structure.
Ashkin, in 1970, first proposed and experimentally demonstrated the use of a laser beam to accelerate and pull particles along an optical axis. This is often considered the earliest experiments to produce "optical tweezers". Since then, the use of light for manipulating and trapping particles has been extensively studied, leading to various new types of "optical tweezers", e.g., "holographic optical tweezers": can be used to manipulate multiple particles simultaneously; "optical fixture": may be used to sort out the target particles. However, the manipulation of the particles by the optical tweezers generated in the prior art is mainly limited to directional movement, adjustable parameters are limited, and fixed-angle rotation of the particles cannot be realized.
Disclosure of Invention
In view of the above, in order to solve the above problems in the prior art, the present invention provides a method and a system for generating high-operability optical tweezers, in which a twisted light beam formed by partially coherent dual off-axis laguerre gaussian superposition is generated to form an optical tweezers with a controllable rotation angle, and the rotation angle of the optical tweezers can be adjusted by a delay system, so as to realize fixed-angle rotation of particles.
The invention solves the problems through the following technical means:
in one aspect, the present invention provides a method for generating high-operability optical tweezers, comprising the following steps:
simulating plane waves and a random electric field with double off-axis Laguerre Gaussian superimposed beams and distorted Gaussian Sheer model information to interfere by using a computer, acquiring a plurality of different phase holograms, and transmitting all the phase holograms to a spatial light modulator;
loading a phase hologram by a spatial light modulator, and converting the loaded phase hologram at a certain refreshing frequency;
starting a laser to emit a linearly polarized Gaussian beam;
the beam expander is used for carrying out collimation and beam expansion processing on the Gaussian beam;
after the beam expanded light beam irradiates the spatial light modulator, filtering is carried out through a 4f system, and a partially coherent double off-axis Laguerre Gaussian superimposed distorted light beam is obtained;
continuously transmitting the partially coherent double off-axis Laguerre Gaussian superimposed distorted light beam after passing through a delay system;
the non-polarization beam splitting cubic mirror performs light splitting processing on the partially coherent dual off-axis Laguerre Gaussian superimposed distorted light beam, one part of the light beam is transmitted to the optical tweezers system, the other part of the light beam is transmitted to the light beam quality analyzer, and the high-operability optical tweezers are obtained in the optical tweezers system.
Further, the specific generation process of the electric field with the dual off-axis laguerre gaussian superimposed beam and the distorted gaussian schell model information is as follows:
Figure 780450DEST_PATH_IMAGE002
E0the electric field is provided with double off-axis Laguerre Gauss superposed beams and distorted Gauss Sheer model information;
wherein
Figure 639251DEST_PATH_IMAGE003
Is the coordinate of the initial plane, x, y are two components orthogonal to each other,
Figure 198408DEST_PATH_IMAGE004
is a coordinate of weight space, where ux,uyTwo components that are orthogonal to each other;
Figure 786516DEST_PATH_IMAGE006
b is a parameter which can be adjusted according to requirements and influences the beam coherence and the distortion factor;
Figure 897560DEST_PATH_IMAGE008
is a kernel function and comprises the following two parts:
Figure 328541DEST_PATH_IMAGE009
is a dual off-axis Laguerre Gauss superimposed beam, C1、C2Is a constant; x is the number of0Representing the distance of the center of two off-axis vortices from the center, w being the beam width, M, N being two adjustable parameters that affect the amount of topological charge, i being the imaginary number
A bit;
Figure 301177DEST_PATH_IMAGE010
a is a distorted Gausser model,
Figure 134003DEST_PATH_IMAGE011
Two adjustable parameters affect the beam coherence and the twist factor.
Furthermore, the rotation rate of the optical potential well, the size of the optical potential well and the distance between the two optical potential wells are regulated and controlled by adjusting the initial optical field parameters:
Figure 917414DEST_PATH_IMAGE013
uby influencing the rate of rotation of the angle of rotation during propagation of the light beam, the rate of rotation of the optical potential well is influenced,uthe larger the beam travels the same distance, the larger the angle turned;
m, N: m and N influence the size of the optical potential well, and the larger M and N are, the larger the optical potential well is;
x0:x0by influencing the spacing, x, of two optical potential wells0The larger the two optical potential wells are spaced the larger.
Further, the 4f system includes two lenses and one diaphragm; the diaphragm is positioned between the two lenses, and the distance from the diaphragm to the two lenses is the focal length of each lens; the first lens performs Fourier transform on the light beam to obtain a frequency spectrum plane; the diaphragm is used for selecting the positive first-order interference fringes of the frequency spectrum surface; the second lens is used for carrying out inverse Fourier change on the light beam selected by the diaphragm to obtain a partially coherent dual off-axis Laguerre Gaussian superimposed distorted light beam.
Further, the time delay system comprises a hollow roof prism and a right-angle prism;
the distance between the hollow ridge prism and the right-angle prism is adjusted, so that the optical path of the light beam entering and exiting the delay system is changed.
Further, the optical tweezers system comprises a dichroic mirror, an objective lens, an objective table, a white light lamp and a complementary metal oxide semiconductor camera;
the partially coherent dual off-axis Laguerre Gaussian superimposed distorted beam is reflected by a dichroic mirror to enter an objective lens, and irradiates on the plane of an objective table, and an optical potential well formed on the plane of the objective table is an optical tweezer; the light beam of the white light lamp after irradiating the particles on the objective table is recorded by the CMOS camera after passing through the dichroic mirror.
In another aspect, the present invention provides a system for generating highly operable optical tweezers, comprising:
the computer is used for simulating the interference of the plane waves and a random electric field with double off-axis Laguerre Gaussian superimposed beams and distorted Gaussian Sieger model information to obtain a plurality of different phase holograms and transmitting all the phase holograms to the spatial light modulator;
the spatial light modulator is used for loading the phase hologram and refreshing the loaded phase hologram at a certain frequency;
a laser for emitting a gaussian beam;
the beam expander is arranged at the exit of the laser and is used for collimating and expanding the Gaussian beam;
the 4f system is used for receiving the light beam reflected by the spatial light modulator and filtering the light beam to obtain a partially coherent double off-axis Laguerre Gaussian superimposed distorted light beam;
the time delay system is used for adjusting the optical path from the partially coherent dual off-axis Laguerre Gaussian superposition distorted light beam initial field to the plane of the objective table of the optical tweezers system to control the rotation angle of the optical potential well;
the optical tweezers system utilizes partially coherent dual off-axis Laguerre Gaussian superposition twisted beams to capture particles;
the beam quality analyzer is arranged behind the delay system and is used for observing the angle rotation condition of the partially coherent double off-axis Laguerre Gauss superposed distorted beam;
the non-polarization beam splitting cubic mirror is arranged between the time delay system and the optical tweezers system and is used for carrying out light splitting processing on the partially coherent double off-axis Laguerre Gaussian superposition twisted light beam, one part of the light beam is transmitted to the optical tweezers system, the other part of the light beam is transmitted to the light beam quality analyzer, and the high-operability optical tweezers are obtained in the optical tweezers system.
Further, the generation system of the high-operability optical tweezers further comprises a plane mirror M1, and the plane mirror M1 is disposed between the beam expander and the spatial light modulator and used for reflecting the expanded light to the spatial light modulator.
Further, the 4f system includes two lenses and one diaphragm; the diaphragm is positioned between the two lenses, and the distance from the diaphragm to the two lenses is the focal length of each lens; the first lens performs Fourier transform on the light beam to obtain a frequency spectrum plane; the diaphragm is used for selecting the positive first-order interference fringes of the frequency spectrum surface; the second lens is used for carrying out inverse Fourier change on the light beam selected by the diaphragm to obtain a partially coherent double off-axis Laguerre Gaussian superimposed distorted light beam;
the time delay system comprises a hollow roof prism and a right-angle prism; the distance between the hollow ridge prism and the right-angle prism is adjusted, so that the optical path of the light beam entering and exiting the delay system is changed;
the optical tweezers system comprises a dichroic mirror, an objective lens, an objective table, a white light lamp and a complementary metal oxide semiconductor camera; the partially coherent dual off-axis Laguerre Gaussian superimposed distorted beam is reflected by a dichroic mirror to enter an objective lens, and irradiates on the plane of an objective table, and an optical potential well formed on the plane of the objective table is an optical tweezer; the light beam of the white light lamp after irradiating the particles on the objective table is recorded by the CMOS camera after passing through the dichroic mirror.
Further, the laser is a 532nm laser and emits a linearly polarized Gaussian beam with the wavelength of 532 nm; the beam expander is a fixed-magnification beam expander with magnification of multiplied by 8;
the spatial light modulator is a reflective spatial light modulator Santec SLM-200, and the resolution is 1900 x 1200; the beam quality analyzer adopts a beam quality analyzer with the highest resolution ratio of 5472 multiplied by 3648 to experimentally obtain the light intensity distribution of cross sections with different transmission distances, thereby realizing the observation of the rotation condition of the optical tweezers.
Compared with the prior art, the invention has the beneficial effects that at least:
1. the system is simple, has lower cost and higher operability in the aspect of particle capture manipulation;
2. according to the invention, the partially coherent dual off-axis Laguerre Gaussian superposition twisted light beam is generated, the vortex phase is ingeniously combined with the special twisted phase of the partially coherent light beam, so that an optical potential well (optical tweezers) with a controllable rotation angle is formed, and the rotation angle of the optical tweezers can be controlled by adjusting the delay system.
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In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a flow chart of the method for producing the high operability optical tweezers according to the present invention;
fig. 2 is a schematic structural diagram of a generation system of the high operability optical tweezers of the present invention;
FIG. 3 is 100 different holographic phase patterns of the present invention;
FIG. 4 is an optical tweezers diagram corresponding to different u values in embodiment 1 of the present invention;
fig. 5 is an optical tweezer diagram corresponding to different u values in embodiment 2 of the present invention.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. It should be noted that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work based on the embodiments of the present invention belong to the protection scope of the present invention.
The flow chart of the method for generating the high-operability optical tweezers by partially coherent double off-axis Laguerre Gaussian superposition distorted light beams through the time delay system is shown in figure 1, and the method for generating the high-operability optical tweezers comprises the following steps:
s1, simulating plane waves and a random electric field with double off-axis Laguerre Gaussian superimposed beams and distorted Gaussian Sheer model information to interfere by using a computer, acquiring 100 different phase holograms, and transmitting all the phase holograms to a reflective spatial light modulator;
s2, loading the phase hologram by the spatial light modulator, and converting the loaded phase hologram at a refresh frequency of 60 HZ;
s3, starting a laser to emit a linearly polarized Gaussian beam;
s4, performing collimation and beam expansion processing on the Gaussian beam by using a beam expander;
s5, filtering the beam after the beam expanded irradiates the spatial light modulator through a 4f system to obtain a partially coherent double off-axis Laguerre Gaussian superimposed distorted beam;
s6, continuously transmitting the partially coherent double off-axis Laguerre Gaussian superimposed distorted light beam after passing through the delay system;
s7, the non-polarization beam splitting cubic mirror carries out beam splitting processing on the partially coherent double off-axis Laguerre Gaussian superposition twisted light beam, one part of the light beam is transmitted to the optical tweezers system, the other part of the light beam is transmitted to the light beam quality analyzer, and the high-operability optical tweezers can be obtained in the optical tweezers system.
It should be noted that the generation process of the random electric field with the information of the dual off-axis laguerre gaussian superimposed beam and the distorted gaussian sierr model is as follows:
Figure 835691DEST_PATH_IMAGE015
E0the electric field is provided with double off-axis Laguerre Gauss superposed beams and distorted Gauss Sheer model information;
wherein
Figure 612017DEST_PATH_IMAGE017
Is the coordinate of the initial plane, x, y are two components orthogonal to each other,
Figure 423984DEST_PATH_IMAGE019
is a coordinate of weight space, where ux,uyTwo components that are orthogonal to each other;
Figure 752198DEST_PATH_IMAGE020
b is a parameter which can be adjusted according to requirements and mainly influences the beam coherence and the distortion factor;
Figure 767558DEST_PATH_IMAGE021
is a kernel function and comprises the following two parts:
Figure 472209DEST_PATH_IMAGE022
is a dual off-axis Laguerre Gauss superimposed beam, C1、C2Is a constant; x is the number of0Representing the distance between the centers of two off-axis vortexes and the center, w is the beam width, M, N is two adjustable parameters which mainly affect the number of topological charges, and i is an imaginary unit;
Figure 640147DEST_PATH_IMAGE023
is a distorted Gaussurer model, A,
Figure 404841DEST_PATH_IMAGE011
Two adjustable parameters mainly affect the beam coherence and the distortion factor.
Specifically, by adjusting initial light field parameters, the rotation rate of the optical potential well, the size of the optical potential well and the distance between the two optical potential wells can be regulated and controlled:
Figure 907498DEST_PATH_IMAGE025
uby passingThe speed of the rotation angle in the light beam propagation process is influenced, so that the rotation speed of the optical potential well is influenced,uthe larger the beam travels the same distance, the larger the angle turned;
m, N: m and N influence the size of the optical potential well, and the larger M and N are, the larger the optical potential well is;
x0:x0by influencing the spacing, x, of two optical potential wells0The larger the two optical potential wells are spaced the larger.
An experimental light path diagram for generating the high-operability optical tweezers is shown in fig. 2, and the high-operability optical tweezers generating system comprises a computer, a helium-neon laser, a beam expander, a spatial light modulator, a non-polarizing beam splitting cubic mirror, a light beam quality analyzer, a 4f system, a time delay system and an optical tweezers system;
the computer is used for simulating interference between the plane waves and a random electric field with double off-axis Laguerre Gaussian superimposed beams and distorted Gaussian Sierr model information to obtain 100 different phase holograms, and transmitting all the phase holograms to the reflective spatial light modulator;
the spatial light modulator is used for loading the phase hologram and refreshing the loaded phase hologram at the frequency of 60 HZ;
the laser is used for emitting linear polarization Gaussian beams;
the beam expander is arranged at the exit of the laser and is used for collimating and expanding the Gaussian beam;
the 4f system is used for receiving the light beams reflected by the spatial light modulator and filtering the light beams to obtain partially coherent double off-axis Laguerre Gaussian superimposed distorted light beams;
the time delay system is used for adjusting the optical path from the partially coherent dual off-axis Laguerre Gaussian superposition twisted beam initial field to the optical tweezers system objective table plane to control the rotation angle of the optical potential well.
The optical tweezers system utilizes partially coherent dual off-axis Laguerre Gaussian superposition twisted beams to capture particles;
the beam quality analyzer is arranged behind the delay system and is used for observing the angle rotation condition of the partially coherent double off-axis Laguerre Gauss superposed distorted beam;
the non-polarization beam splitting cubic mirror is arranged between the time delay system and the optical tweezers system and is used for splitting the partially coherent double off-axis Laguerre Gaussian superposition twisted light beam, one part of the light beam is transmitted to the optical tweezers system, the other part of the light beam is transmitted to the light beam quality analyzer, and the high-operability optical tweezers can be obtained in the optical tweezers system.
Specifically, the generation system of the high-operability optical tweezers further comprises a plane mirror M1, and the plane mirror M1 is disposed between the beam expander and the spatial light modulator and used for reflecting the expanded light to the spatial light modulator.
Specifically, the 4f system includes two lenses and one diaphragm; the diaphragm is positioned between the two lenses, and the distance between the diaphragm and the two lenses is the focal length of each lens; the first lens performs Fourier transform on the light beam to obtain a frequency spectrum plane; the diaphragm is used for selecting the positive first-order interference fringes of the frequency spectrum surface; the second lens is used for carrying out inverse Fourier change on the light beam selected by the diaphragm to obtain a partially coherent double off-axis Laguerre Gaussian superimposed distorted light beam.
Specifically, the time delay system comprises a hollow roof prism and a right-angle prism; the distance between the hollow ridge prism and the right-angle prism is adjusted, so that the optical path of the light beam entering and exiting the delay system is changed.
Specifically, the optical tweezers system comprises a dichroic mirror, an objective lens, an objective table, a white light lamp and a complementary metal oxide semiconductor camera (CMSO camera); the partially coherent dual off-axis Laguerre Gaussian superimposed distorted beam is reflected by a dichroic mirror to enter an objective lens, and irradiates on the plane of an objective table, and an optical potential well formed on the plane of the objective table is an optical tweezer; and the light beam of the white light lamp after irradiating the particles on the objective table is recorded by the CMSO camera after passing through the dichroic mirror.
Specifically, the laser is a 532nm laser, and emits a linearly polarized Gaussian beam with the wavelength of 532 nm.
Specifically, the magnification of the laser beam expander with fixed magnification is × 8, and the beam expander performs collimation and beam expansion processing on the gaussian beam.
In particular, the spatial light modulator is a reflective spatial light modulator Santec SLM-200 with a resolution of 1900 × 1200 for loading the computer generated phase hologram and refreshing the loaded phase hologram at a frequency of 60 Hz.
Specifically, the beam quality analyzer with the resolution as high as 5472 × 3648 is adopted, and cross-section light intensity distributions of different transmission distances can be obtained experimentally, so that the rotation condition of the optical tweezers can be observed.
The invention is illustrated below with specific experimental data.
Example 1
Example 1 is consistent with fig. 4, under different u values, the partially coherent dual off-axis laguerre gaussian superposition distorts the beam to propagate the same distance, and the optical potential wells (dark holes in the figure) rotate at different angles. Along with the increase of u, the light beams propagate for the same distance, and the larger the rotating angle of the optical potential well is, the optical tweezers with controllable rotating angles can be formed in the optical tweezers system.
S1, the numerical simulation plane wave and the parameters are M =2, N =2, x0And =2, w =1 holographic phase diagrams with interference of double off-axis laguerre gaussian superimposed beams and random electric fields of distorted gaussian schell model information, and u is set to be 2, 3 and 4 respectively.
S2, loading the acquired holographic phase pattern into the spatial light modulator as shown in fig. 3, and refreshing the loaded holographic phase pattern at a frequency of 60 HZ.
S3, using the experimental optical path diagram of fig. 2, it is possible to form highly operable optical tweezers in the optical tweezers system, and the optical tweezers corresponding to different u values are as shown in fig. 4.
Example 2
Example 2 in agreement with fig. 5, the two optical potential wells (dark holes in the figure) of the N-scaled down, partially coherent dual off-axis laguerre gaussian superposition distorted beam were made smaller one and kept the same one. This can form optical tweezers capable of manipulating different amounts of particles in the optical tweezers system.
S1, the numerical simulation plane wave and the parameters are M =2, N =1, x0=2, w =1 with double separationAnd (3) setting u as 2, 3 and 4 respectively for the holographic phase images after the interference of the Axis Gaguerre Gaussian superposed beam and the random electric field of the distorted Gaussian Sheer model information.
And S2, loading the acquired holographic phase pattern into the spatial light modulator, and refreshing the loaded holographic phase pattern at the frequency of 60 HZ.
S3, using the experimental optical path diagram of fig. 2, it is possible to form highly operable optical tweezers in the optical tweezers system, and the optical tweezers corresponding to different u values are as shown in fig. 5.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. A method for generating high-operability optical tweezers is characterized by comprising the following steps:
simulating plane waves and a random electric field with double off-axis Laguerre Gaussian superimposed beams and distorted Gaussian Sheer model information to interfere by using a computer, acquiring a plurality of different phase holograms, and transmitting all the phase holograms to a spatial light modulator;
loading a phase hologram by a spatial light modulator, and converting the loaded phase hologram at a certain refreshing frequency;
starting a laser to emit a linearly polarized Gaussian beam;
the beam expander is used for carrying out collimation and beam expansion processing on the Gaussian beam;
after the beam expanded light beam irradiates the spatial light modulator, filtering is carried out through a 4f system, and a partially coherent double off-axis Laguerre Gaussian superimposed distorted light beam is obtained;
continuously transmitting the partially coherent double off-axis Laguerre Gaussian superimposed distorted light beam after passing through a delay system;
the non-polarization beam splitting cubic mirror performs light splitting processing on the partially coherent dual off-axis Laguerre Gaussian superimposed distorted light beam, one part of the light beam is transmitted to the optical tweezers system, the other part of the light beam is transmitted to the light beam quality analyzer, and the high-operability optical tweezers are obtained in the optical tweezers system.
2. The method for generating optical tweezers with high operability according to claim 1, wherein the specific generation process of the electric field with the dual off-axis laguerre gaussian superimposed beam and the distorted gaussian schell model information is as follows:
Figure 468013DEST_PATH_IMAGE001
E0the electric field is provided with double off-axis Laguerre Gauss superposed beams and distorted Gauss Sheer model information;
wherein
Figure 833398DEST_PATH_IMAGE002
Is the coordinate of the initial plane, x, y are two components orthogonal to each other,
Figure 802491DEST_PATH_IMAGE003
is a coordinate of a weight space, whereinu xu yTwo components that are orthogonal to each other;
Figure 160791DEST_PATH_IMAGE004
b is a parameter which can be adjusted according to requirements and influences the beam coherence and the distortion factor;
Figure 289153DEST_PATH_IMAGE005
is a kernel function and comprises the following two parts:
Figure 933761DEST_PATH_IMAGE006
is a dual off-axis Laguerre Gauss superimposed beam, C1、C2Is a constant; x is the number of0Representing the distance of the center of two off-axis vortices from the center, w being the beam width, M, N being two adjustable parameters that affect the amount of topological charge, i being the imaginary number
A bit;
Figure 531096DEST_PATH_IMAGE007
is a distorted Gaussurer model, A,
Figure 912661DEST_PATH_IMAGE008
Two adjustable parameters affect the beam coherence and the twist factor.
3. The method for generating highly operational optical tweezers of claim 2, wherein the adjustment of the rotation rate of the optical potential wells, the size of the optical potential wells and the distance between two optical potential wells is achieved by adjusting the initial optical field parameters:
Figure 770895DEST_PATH_IMAGE009
uthe rate of rotation of the optical potential well is influenced in response to the rate of rotation of the angle of rotation during propagation of the beam,uthe larger the beam travels the same distance, the larger the angle turned;
m, N: m and N influence the size of the optical potential well, and the larger M and N are, the larger the optical potential well is;
x0 :x0by influencing the spacing, x, of two optical potential wells0The larger the two optical potential wells are spaced the larger.
4. The method of claim 1, wherein the 4f system comprises two lenses and a stop; the diaphragm is positioned between the two lenses, and the distance from the diaphragm to the two lenses is the focal length of each lens; the first lens performs Fourier transform on the light beam to obtain a frequency spectrum plane; the diaphragm is used for selecting the positive first-order interference fringes of the frequency spectrum surface; the second lens is used for carrying out inverse Fourier change on the light beam selected by the diaphragm to obtain a partially coherent dual off-axis Laguerre Gaussian superimposed distorted light beam.
5. The method of generating high operability optical tweezers of claim 1, wherein the time delay system comprises a hollow roof prism and a right angle prism;
the distance between the hollow ridge prism and the right-angle prism is adjusted, so that the optical path of the light beam entering and exiting the delay system is changed.
6. The method for generating high operability optical tweezers of claim 1, wherein the optical tweezers system comprises a dichroic mirror, an objective lens, an object stage, a white light lamp and a cmos camera;
the partially coherent dual off-axis Laguerre Gaussian superimposed distorted beam is reflected by a dichroic mirror to enter an objective lens, and irradiates on the plane of an objective table, and an optical potential well formed on the plane of the objective table is an optical tweezer; the light beam of the white light lamp after irradiating the particles on the objective table is recorded by the CMOS camera after passing through the dichroic mirror.
7. A system for generating highly operational optical tweezers, comprising:
the computer is used for simulating the interference of the plane waves and a random electric field with double off-axis Laguerre Gaussian superimposed beams and distorted Gaussian Sieger model information to obtain a plurality of different phase holograms and transmitting all the phase holograms to the spatial light modulator;
the spatial light modulator is used for loading the phase hologram and refreshing the loaded phase hologram at a certain frequency;
a laser for emitting a gaussian beam;
the beam expander is arranged at the exit of the laser and is used for collimating and expanding the Gaussian beam;
the 4f system is used for receiving the light beam reflected by the spatial light modulator and filtering the light beam to obtain a partially coherent double off-axis Laguerre Gaussian superimposed distorted light beam;
the time delay system is used for adjusting the optical path from the partially coherent dual off-axis Laguerre Gaussian superposition distorted light beam initial field to the plane of the objective table of the optical tweezers system to control the rotation angle of the optical potential well;
the optical tweezers system utilizes partially coherent dual off-axis Laguerre Gaussian superposition twisted beams to capture particles;
the beam quality analyzer is arranged behind the delay system and is used for observing the angle rotation condition of the partially coherent double off-axis Laguerre Gauss superposed distorted beam;
the non-polarization beam splitting cubic mirror is arranged between the time delay system and the optical tweezers system and is used for carrying out light splitting processing on the partially coherent double off-axis Laguerre Gaussian superposition twisted light beam, one part of the light beam is transmitted to the optical tweezers system, the other part of the light beam is transmitted to the light beam quality analyzer, and the high-operability optical tweezers are obtained in the optical tweezers system.
8. The generation system of high operability optical tweezers of claim 7, further comprising a plane mirror M1, wherein the plane mirror M1 is disposed between the beam expander and the spatial light modulator, and is configured to reflect the expanded light to the spatial light modulator.
9. The generation system of highly operational optical tweezers of claim 7, wherein the 4f system comprises two lenses and one stop; the diaphragm is positioned between the two lenses, and the distance between the diaphragm and the two lenses is the focal length of each lens; the first lens performs Fourier transform on the light beam to obtain a frequency spectrum surface; the diaphragm is used for selecting the positive first-order interference fringes of the frequency spectrum surface; the second lens is used for carrying out inverse Fourier change on the light beam selected by the diaphragm to obtain a partially coherent double off-axis Laguerre Gaussian superimposed distorted light beam;
the time delay system comprises a hollow roof prism and a right-angle prism; the distance between the hollow ridge prism and the right-angle prism is adjusted, so that the optical path of the light beam entering and exiting the delay system is changed;
the optical tweezers system comprises a dichroic mirror, an objective lens, an objective table, a white light lamp and a complementary metal oxide semiconductor camera; the partially coherent dual off-axis Laguerre Gaussian superimposed distorted beam is reflected by a dichroic mirror to enter an objective lens, and irradiates on the plane of an objective table, and an optical potential well formed on the plane of the objective table is an optical tweezer; the light beam of the white light lamp after irradiating the particles on the objective table is recorded by the CMOS camera after passing through the dichroic mirror.
10. The generation system of highly operational optical tweezers of claim 7, wherein the laser is a 532nm laser emitting a linearly polarized gaussian beam of wavelength 532 nm; the beam expander is a fixed-magnification beam expander with magnification of multiplied by 8;
the spatial light modulator is a reflective spatial light modulator Santec SLM-200, and the resolution is 1900 x 1200; the beam quality analyzer adopts a beam quality analyzer with the resolution as high as 5472X 3648, and cross-section light intensity distribution of different transmission distances is obtained experimentally, so that the condition of rotation of the optical tweezers is observed.
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