CN114480511A - Method for preparing methane by utilizing straw anaerobic fermentation - Google Patents
Method for preparing methane by utilizing straw anaerobic fermentation Download PDFInfo
- Publication number
- CN114480511A CN114480511A CN202210206642.1A CN202210206642A CN114480511A CN 114480511 A CN114480511 A CN 114480511A CN 202210206642 A CN202210206642 A CN 202210206642A CN 114480511 A CN114480511 A CN 114480511A
- Authority
- CN
- China
- Prior art keywords
- anaerobic fermentation
- straw
- corn
- parts
- methane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 92
- 239000010902 straw Substances 0.000 title claims abstract description 82
- 238000000855 fermentation Methods 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 35
- 239000007788 liquid Substances 0.000 claims abstract description 73
- 240000008042 Zea mays Species 0.000 claims abstract description 65
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 65
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 65
- 235000005822 corn Nutrition 0.000 claims abstract description 65
- 230000001580 bacterial effect Effects 0.000 claims abstract description 56
- 239000001963 growth medium Substances 0.000 claims abstract description 24
- 239000002054 inoculum Substances 0.000 claims abstract description 21
- 238000002156 mixing Methods 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000011259 mixed solution Substances 0.000 claims abstract description 17
- 238000012258 culturing Methods 0.000 claims abstract description 15
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 12
- 241000378866 Trichoderma koningii Species 0.000 claims description 11
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims description 10
- 235000007685 Pleurotus columbinus Nutrition 0.000 claims description 10
- 240000001462 Pleurotus ostreatus Species 0.000 claims description 10
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims description 10
- 230000004151 fermentation Effects 0.000 claims description 9
- 241000222355 Trametes versicolor Species 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 6
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims description 6
- 239000011790 ferrous sulphate Substances 0.000 claims description 6
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 6
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 6
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 238000007873 sieving Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000011592 zinc chloride Substances 0.000 claims description 6
- 235000005074 zinc chloride Nutrition 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 5
- 241000894007 species Species 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 abstract description 5
- 238000002360 preparation method Methods 0.000 abstract description 2
- 238000003756 stirring Methods 0.000 description 17
- 230000015556 catabolic process Effects 0.000 description 14
- 238000006731 degradation reaction Methods 0.000 description 14
- 230000009471 action Effects 0.000 description 9
- 239000002994 raw material Substances 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000001913 cellulose Substances 0.000 description 5
- 229920002678 cellulose Polymers 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 229920005610 lignin Polymers 0.000 description 5
- 239000010907 stover Substances 0.000 description 5
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 239000010802 sludge Substances 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 241000222354 Trametes Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000123346 Chrysosporium Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides a method for preparing methane by utilizing straw anaerobic fermentation, which relates to the technical field of methane preparation, and comprises the following steps: performing propagation culture on the first strain on a first corn straw culture medium by 15-18% of inoculation amount to obtain a first bacterial liquid; performing propagation culture on a second corn straw culture medium by using 15-18% of inoculum size of a second strain to obtain a second bacterial liquid; mixing corn straws, inorganic salt and water uniformly to obtain a first mixed solution; adding 16-20 parts by mass of the first bacterial liquid into the first mixed liquid, culturing at 32-35 ℃ for 2.5-3.5 days, adding 8-10 parts by mass of the second bacterial liquid, and continuously culturing for 7.5-8.5 days to obtain a second mixed liquid; and adding the inoculum into the second mixed solution, uniformly mixing, and performing anaerobic fermentation to obtain methane. The method has the advantages of mild condition, easy control, capability of improving the yield of methane and low cost.
Description
Technical Field
The invention relates to the technical field of methane preparation, in particular to a method for preparing methane by utilizing straw anaerobic fermentation.
Background
As a big agricultural country in China, the corn straws are produced in huge quantities every year, so that the resource utilization of the corn straws has become a key point of attention. The production of methane by anaerobic fermentation technology is one of the most effective methods for resource utilization. But the substrate utilization and methane production of anaerobic fermentation are largely limited due to the presence of large amounts of lignin and its structural properties.
In the prior art, the yield of methane can be improved by acid-base pretreatment and other modes, but the problems of high equipment requirement and high cost exist at the same time, and biological pretreatment is one of the cleanest and ideal modes at present. However, the degradation of corn straws by using microorganisms such as white rot fungi, bacteria and the like also has the problems of low degradation efficiency, long time and excessive loss of reducing sugars such as cellulose, hemicellulose and the like.
Disclosure of Invention
The invention solves the technical problem of how to prepare high-yield methane by utilizing straw anaerobic fermentation.
In order to solve the problems, the invention provides a method for preparing methane by utilizing straw anaerobic fermentation, which comprises the following steps:
step S1, performing propagation culture on the first strain on a first corn straw culture medium by 15-18% of inoculum size to obtain a first bacterial liquid; performing propagation culture on a second corn straw culture medium by using 15-18% of inoculum size of a second strain to obtain a second bacterial liquid;
step S2, mixing corn straw, inorganic salt and water uniformly to obtain a first mixed solution;
step S3, adding 16-20 parts by mass of the first bacterial liquid into the first mixed liquid, culturing at 32-35 ℃ for 2.5-3.5 days, adding 8-10 parts by mass of the second bacterial liquid, and continuously culturing for 7.5-8.5 days to obtain a second mixed liquid;
and step S4, adding the inoculum into the second mixed solution, uniformly mixing, and performing anaerobic fermentation to obtain methane.
Optionally, in step S1, the first bacterial species includes phanerochaete chrysosporium, trametes versicolor and pleurotus ostreatus.
Optionally, the mass ratio of the phanerochaete chrysosporium to the tramete versicolor and the pleurotus ostreatus is 1: 1: 1.
optionally, in step S1, the second species comprises trichoderma koningii.
Optionally, in step S1, the solid-to-liquid ratio of the first corn stalk culture medium and the second corn stalk culture medium ranges from 12% to 18%, and the PH ranges from 4.3 to 4.8.
Optionally, in step S1, the time range of the propagation culture includes 8.5-9.5d, and the temperature range of the propagation culture includes 28-35 ℃.
Optionally, in step S2, the blending corn stover, inorganic salt, and water includes: uniformly mixing corn straws, inorganic salt and water according to the following parts by mass: 10-15 parts of corn straw, 2-3 parts of ammonium sulfate, 0.002-0.003 part of cobalt chloride, 0.003-0.008 part of ferrous sulfate, 0.15-0.2 part of zinc chloride, 0.2-0.5 part of magnesium sulfate, 0.2-0.5 part of calcium chloride, 2-3 parts of monopotassium phosphate, 0.5-1 part of sodium chloride, 0.015-0.2 part of manganese sulfate and 950-1050 parts of water.
Optionally, in step S2, the corn stalks need to be pretreated, and the pretreatment process includes: airing and crushing the corn straws, and sieving the corn straws with a 35-50-mesh sieve.
Optionally, in step S4, the adding the inoculum to the second mixture includes: adding inoculum to the second mixed liquor until the total solid content of the second mixed liquor ranges from 6 to 8 percent.
Optionally, in step S4, the anaerobic fermentation conditions include: the fermentation temperature range is 33-37 ℃, and the fermentation period is 28-32 days.
Compared with the prior art, the method for preparing methane by utilizing straw anaerobic fermentation has the following beneficial effects:
1) treating corn straws by using microorganisms: on one hand, the hydrolysis effect is better, the methane yield is improved by 30%, on the other hand, the conditions are mild and easy to control, and acid and alkali resistant and high-temperature and high-pressure resistant equipment and instruments are not needed, so that the pretreatment cost is reduced, and the possibility of environmental pollution is reduced;
2) the pretreated corn straws are directly added with inocula for anaerobic fermentation, and filtering or washing for adjusting pH is not needed in the process, so that water consumption is saved, and reducing sugar hydrolyzed by microorganisms can be utilized to the maximum extent.
3) The first strain and the second strain are used for improving the degradation effect of lignin and cellulose in a synergistic mode, the first bacterium liquid is firstly accessed, then the second bacterium liquid is accessed, the phenomenon that degradation capacity is reduced due to the fact that nutrition is contended for by the first bacterium liquid and the second bacterium liquid when the first bacterium liquid and the second bacterium liquid are accessed simultaneously is avoided, and the degradation efficiency is further improved.
Drawings
FIG. 1 is a flow chart of a method for preparing methane by utilizing straw anaerobic fermentation in the embodiment of the invention.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
In the description of the present invention, it is to be understood that the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature.
The description of the term "some embodiments" means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. Throughout this specification, the schematic representations of the terms used above do not necessarily refer to the same implementation or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
As shown in figure 1, the embodiment of the invention provides a method for preparing methane by utilizing straw anaerobic fermentation, which comprises the following steps:
step S1, performing propagation culture on the first strain on a first corn straw culture medium by 15-18% of inoculum size to obtain a first bacterial liquid; performing propagation culture on a second corn straw culture medium by using 15-18% of inoculum size of a second strain to obtain a second bacterial liquid;
step S2, mixing corn straw, inorganic salt and water uniformly to obtain a first mixed solution;
step S3, adding 16-20 parts by mass of the first bacterial liquid into the first mixed liquid, culturing at 32-35 ℃ for 2.5-3.5 days, adding 8-10 parts by mass of the second bacterial liquid, and continuously culturing for 7.5-8.5 days to obtain a second mixed liquid;
and step S4, adding the inoculum into the second mixed solution, uniformly mixing, and performing anaerobic fermentation to obtain methane.
In some embodiments, in step S1, the first species includes phanerochaete chrysosporium, trametes versicolor, and pleurotus ostreatus. Therefore, the first bacterial liquid prepared from the phanerochaete chrysosporium, the tramete versicolor and the pleurotus ostreatus can solve the problem of low degradation capability of a single strain caused by growth characteristics, period and lack of nutrients, and improves the degradation rate of lignin.
In some specific embodiments, the ratio by mass of the phanerochaete chrysosporium, the trametes versicolor and the pleurotus ostreatus is 1: 1: 1. therefore, the quality balance of each strain is ensured.
In a preferred embodiment, in step S1, the second species comprises trichoderma koningii. Therefore, the second bacterial liquid prepared from trichoderma koningii can degrade cellulose to become glucose, and provides an effective carbon source for microorganisms in the subsequent anaerobic fermentation process.
In some embodiments, in step S1, the first corn stover medium and the second corn stover medium have a solid to liquid ratio in a range comprising 12 to 18% and a pH in a range comprising 4.3 to 4.8. Therefore, the first bacterial liquid and the second bacterial liquid with proper pH value can be obtained, so that the corn straws can be better treated.
In some embodiments, in step S1, the time range of the propagation culture includes 8.5-9.5d, and the temperature range of the propagation culture includes 28-35 ℃. Therefore, the obtained first bacterial liquid and the second bacterial liquid can promote the hydrolysis of the corn straws to the maximum extent, and high-yield methane is obtained.
In some embodiments, in step S2, the blending corn stover, inorganic salt, and water comprises: uniformly mixing corn straws, inorganic salt and water according to the following parts by mass: 10-15 parts of corn straw, 2-3 parts of ammonium sulfate, 0.002-0.003 part of cobalt chloride, 0.003-0.008 part of ferrous sulfate, 0.15-0.2 part of zinc chloride, 0.2-0.5 part of magnesium sulfate, 0.2-0.5 part of calcium chloride, 2-3 parts of monopotassium phosphate, 0.5-1 part of sodium chloride, 0.015-0.2 part of manganese sulfate and 950-1050 parts of water. Thereby, sufficient inorganic salts and trace elements are provided.
In some embodiments, in step S2, the corn stover is subjected to a pretreatment process comprising: airing and crushing the corn straws, and sieving the corn straws with a 35-50-mesh sieve. Therefore, the corn straws and other raw materials are mixed more uniformly, and hydrolysis is more sufficient.
It should be noted that, in step S3 in this embodiment, the first bacterial liquid needs to be added to the first mixed liquid, and after the first bacterial liquid is cultured at 32-35 ℃ for 2.5-3.5 days, the second bacterial liquid is added, and the second bacterial liquid is continuously cultured for 7.5-8.5 days, so that the phenomenon that the degradation capability of trichoderma koningii is reduced due to the growth and maturation of the trichoderma chrysosporium and the nutrition competition with trichoderma koningii as the fermentation time is prolonged when the trichoderma koningii and the trichoderma koningii are inoculated simultaneously is avoided.
In addition, in this embodiment, it is necessary to mix a stirring device in the culturing process of step S1 and step S3. In some preferred embodiments, the rotation speed range of the stirring device comprises 160-170 r/min. Thereby, the mixing is more uniform and time is saved.
In some embodiments, the adding an inoculum to the second mixture in step S4 includes: adding inoculum to the second mixed liquor until the total solid content of the second mixed liquor ranges from 6 to 8 percent. Therefore, the method can avoid overhigh organic load and is more beneficial to starting anaerobic reaction.
In some preferred embodiments, the inoculum is sludge, which is more conducive to anaerobic fermentation.
In some embodiments, in step S4, the conditions of the anaerobic fermentation include: the fermentation temperature range comprises 33-37 ℃, so that the temperature is more suitable for anaerobic fermentation and has better economic performance.
In some embodiments, in step S4, the conditions for anaerobic fermentation further comprise: the fermentation period comprises 28-32d, thereby ensuring the complete anaerobic fermentation process.
Therefore, the method for preparing methane by using straw anaerobic fermentation described in this embodiment uses microorganisms to treat corn straws: on one hand, the hydrolysis effect is better, the methane yield is improved by 30%, on the other hand, the conditions are mild and easy to control, and acid and alkali resistant and high-temperature and high-pressure resistant equipment and instruments are not needed, so that the pretreatment cost is reduced, and the possibility of environmental pollution is reduced; and the pretreated corn straws are directly added with the inoculum for anaerobic fermentation, and the filtration or flushing for adjusting the pH value is not needed in the process, so that the water consumption is saved, and simultaneously, the reducing sugar hydrolyzed by the microorganism can be utilized to the maximum extent. Meanwhile, the degradation effect of lignin and cellulose is improved by the aid of the first strain and the second strain in a synergistic mode, the first bacterial liquid is connected first, the second bacterial liquid is connected later, the phenomenon that degradation capacity is reduced due to the fact that nutrition is contended for by the first bacterial liquid and the second bacterial liquid when the first bacterial liquid and the second bacterial liquid are connected simultaneously is avoided, and degradation efficiency is further improved.
Example 1
The method for preparing methane by utilizing straw anaerobic fermentation provided by the embodiment comprises the following steps:
step S1, under the action of a stirring device, carrying out propagation culture on a first corn straw culture medium for 8.5 days by using 15% of inoculum size of a first strain (Phanerochaete chrysosporium, trametes versicolor and Pleurotus ostreatus in a mass ratio of 1: 1: 1) at the temperature of 28 ℃ to obtain a first bacterial liquid; under the action of a stirring device, performing propagation culture on trichoderma koningii on a second corn straw culture medium at the temperature of 28 ℃ for 8.5 days by 15% of inoculation amount to obtain a second bacterial liquid, wherein the rotating speed of the stirring device is 160r/min, the solid-liquid ratio of the first corn straw culture medium to the second corn straw culture medium is 12%, and the pH range is 4.3;
step S2, uniformly mixing the following raw materials in parts by mass to obtain a first mixed solution, wherein the raw materials comprise: 10 parts of corn straw, 2 parts of ammonium sulfate, 0.002 part of cobalt chloride, 0.003 part of ferrous sulfate, 0.15 part of zinc chloride, 0.2 part of magnesium sulfate, 0.2 part of calcium chloride, 3 parts of monopotassium phosphate, 0.5 part of sodium chloride, 0.1 part of manganese sulfate and 950 parts of water, wherein the corn straw is obtained by drying, crushing and sieving with a 35-mesh sieve;
step S3, under the action of a stirring device, adding 16 parts by mass of the first bacterial liquid into the first mixed liquid, culturing at the temperature of 32 ℃ for 2.5 days, adding 8 parts by mass of the second bacterial liquid, and continuously culturing for 7.5 days to obtain a second mixed liquid, wherein the rotating speed of the stirring device is 160 r/min;
and step S4, adding inoculated sludge into the second mixed solution until the total solid content range of the second mixed solution is 6%, uniformly mixing, and performing anaerobic fermentation for 28d at the temperature of 33 ℃ to obtain methane.
Example 2
The method for preparing methane by utilizing straw anaerobic fermentation provided by the embodiment comprises the following steps:
step S1, under the action of a stirring device, carrying out propagation culture on a first corn straw culture medium for 9d by a first strain (Phanerochaete chrysosporium, trametes versicolor and Pleurotus ostreatus with a mass ratio of 1: 1: 1) at a temperature of 32 ℃ by an inoculation amount of 16% to obtain a first bacterial liquid; under the action of a stirring device, performing propagation culture on trichoderma koningii on a second corn straw culture medium at the temperature of 32 ℃ for 9d by using 16% of inoculation amount to obtain a second bacterial liquid, wherein the rotating speed of the stirring device is 165r/min, the solid-liquid ratio of the first corn straw culture medium to the second corn straw culture medium is 15%, and the pH range is 4.6;
step S2, uniformly mixing the following raw materials in parts by mass to obtain a first mixed solution, wherein the raw materials comprise: 12 parts of corn straws, 2.5 parts of ammonium sulfate, 0.002 part of cobalt chloride, 0.005 part of ferrous sulfate, 0.15 part of zinc chloride, 0.3 part of magnesium sulfate, 0.3 part of calcium chloride, 2 parts of potassium dihydrogen phosphate, 0.8 part of sodium chloride, 0.1 part of manganese sulfate and 1000 parts of water, wherein the corn straws are obtained by airing and crushing and then sieving through a 40-mesh sieve;
step S3, under the action of a stirring device, adding 18 parts by weight of the first bacterial liquid into the first mixed liquid, culturing at 34 ℃ for 3 days, adding 8 parts by weight of the second bacterial liquid, and continuously culturing for 8 days to obtain a second mixed liquid, wherein the rotating speed of the stirring device is 165 r/min;
and step S4, adding inoculated sludge into the second mixed solution until the total solid content range of the second mixed solution is 6%, uniformly mixing, and performing anaerobic fermentation at the temperature of 35 ℃ for 30d to obtain methane.
Example 3
The method for preparing methane by utilizing straw anaerobic fermentation provided by the embodiment comprises the following steps:
step S1, under the action of a stirring device, carrying out propagation culture on a first corn straw culture medium for 9.5 days by using 18% of inoculum size of a first strain (Phanerochaete chrysosporium, trametes versicolor and Pleurotus ostreatus in a mass ratio of 1: 1: 1) at the temperature of 35 ℃ to obtain a first bacterial liquid; under the action of a stirring device, performing propagation culture on trichoderma koningii on a second corn straw culture medium at the temperature of 35 ℃ for 9.5 days by using 18% of inoculation amount to obtain a second bacterial liquid, wherein the rotating speed of the stirring device is 170r/min, the solid-liquid ratio of the first corn straw culture medium to the second corn straw culture medium is 18%, and the pH range value is 4.8;
step S2, uniformly mixing the following raw materials in parts by mass to obtain a first mixed solution, wherein the raw materials comprise: 15 parts of corn straw, 3 parts of ammonium sulfate, 0.003 part of cobalt chloride, 0.008 part of ferrous sulfate, 0.2 part of zinc chloride, 0.5 part of magnesium sulfate, 0.5 part of calcium chloride, 3 parts of monopotassium phosphate, 1 part of sodium chloride, 0.2 part of manganese sulfate and 1050 parts of water, wherein the corn straw is obtained by airing, crushing and sieving with a 50-mesh sieve;
step S3, under the action of a stirring device, adding 20 parts by mass of the first bacterial liquid into the first mixed liquid, culturing at 35 ℃ for 3.5 days, adding 10 parts by mass of the second bacterial liquid, and continuously culturing for 8.5 days to obtain a second mixed liquid, wherein the rotating speed of the stirring device is 170 r/min;
and step S4, adding inoculated sludge into the second mixed solution until the total solid content range of the second mixed solution is 8%, uniformly mixing, and performing anaerobic fermentation for 32d at the temperature of 37 ℃ to obtain methane.
Comparative example
In order to verify the effectiveness of the method for preparing methane by using straw anaerobic fermentation in the invention, the invention takes the method steps of preparing methane by using straw anaerobic fermentation in the embodiment 2 as the basis, only adjusts raw materials or processes under the condition of not changing other experimental conditions, adopts various methods as comparative examples to prepare methane, wherein the comparative example 5 is an embodiment for preparing methane by using corn straw anaerobic fermentation without treating with first bacterial liquid or second bacterial liquid, and the accumulated methane yield prepared by the embodiment 2 and a plurality of comparative examples is as follows.
As can be seen from the above table, the methane yield is the highest in example 2, and is increased by 30% compared with that in comparative example 5, and in example 2, the first bacterial strain and the second bacterial strain are used to synergistically improve the degradation effects of lignin and cellulose, the effect is better, and the first bacterial solution is accessed first and then the second bacterial solution is accessed, so that the phenomenon that the degradation capability is reduced due to the fact that the first bacterial solution and the second bacterial solution compete for nutrients when the first bacterial solution and the second bacterial solution are accessed simultaneously is avoided, the degradation efficiency is further improved, and the methane yield is increased.
Although the present invention is disclosed above, the present invention is not limited thereto. Various changes and modifications may be effected therein by one skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. A method for preparing methane by utilizing straw anaerobic fermentation is characterized by comprising the following steps:
step S1, performing propagation culture on the first strain on a first corn straw culture medium by 15-18% of inoculum size to obtain a first bacterial liquid; performing propagation culture on a second corn straw culture medium by using 15-18% of inoculum size of a second strain to obtain a second bacterial liquid;
step S2, mixing corn straw, inorganic salt and water uniformly to obtain a first mixed solution;
step S3, adding 16-20 parts by mass of the first bacterial liquid into the first mixed liquid, culturing at 32-35 ℃ for 2.5-3.5 days, adding 8-10 parts by mass of the second bacterial liquid, and continuously culturing for 7.5-8.5 days to obtain a second mixed liquid;
and step S4, adding the inoculum into the second mixed solution, uniformly mixing, and performing anaerobic fermentation to obtain methane.
2. The method for preparing methane by using straw anaerobic fermentation according to claim 1, wherein in step S1, the first bacterial species includes Phanerochaete chrysosporium, trametes versicolor and Pleurotus ostreatus.
3. The method for preparing methane by utilizing straw anaerobic fermentation as claimed in claim 2, wherein the mass ratio of the phanerochaete chrysosporium to the trametes versicolor to the pleurotus ostreatus is 1: 1: 1.
4. the method for preparing methane by using straw anaerobic fermentation according to claim 1, wherein in step S1, the second strain comprises Trichoderma koningii.
5. The method for preparing methane by using straw anaerobic fermentation of claim 1, wherein in step S1, the solid-to-liquid ratio of the first corn straw culture medium and the second corn straw culture medium is in the range of 12-18%, and the pH value is in the range of 4.3-4.8.
6. The method for preparing methane by using straw anaerobic fermentation as claimed in claim 1, wherein in step S1, the time range of the propagation culture comprises 8.5-9.5d, and the temperature range of the propagation culture comprises 28-35 ℃.
7. The method for preparing methane by using straw anaerobic fermentation according to claim 1, wherein in step S2, the step of mixing corn straw, inorganic salt and water comprises: uniformly mixing corn straws, inorganic salt and water according to the following parts by mass: 10-15 parts of corn straw, 2-3 parts of ammonium sulfate, 0.002-0.003 part of cobalt chloride, 0.003-0.008 part of ferrous sulfate, 0.15-0.2 part of zinc chloride, 0.2-0.5 part of magnesium sulfate, 0.2-0.5 part of calcium chloride, 2-3 parts of monopotassium phosphate, 0.5-1 part of sodium chloride, 0.015-0.2 part of manganese sulfate and 950-1050 parts of water.
8. The method for preparing methane by using straw anaerobic fermentation according to claim 1 or 7, wherein in step S2, the corn straw needs to be pretreated, and the pretreatment process comprises the following steps: airing and crushing the corn straws, and sieving the corn straws with a 35-50-mesh sieve.
9. The method for preparing methane by using straw anaerobic fermentation according to claim 1, wherein in step S4, the adding of the inoculum to the second mixed liquor comprises: adding inoculum to the second mixed liquor until the total solid content of the second mixed liquor ranges from 6 to 8 percent.
10. The method for preparing methane by using straw anaerobic fermentation according to claim 1, wherein in step S4, the anaerobic fermentation conditions comprise: the fermentation temperature range is 33-37 ℃, and the fermentation period is 28-32 d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210206642.1A CN114480511A (en) | 2022-03-03 | 2022-03-03 | Method for preparing methane by utilizing straw anaerobic fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210206642.1A CN114480511A (en) | 2022-03-03 | 2022-03-03 | Method for preparing methane by utilizing straw anaerobic fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114480511A true CN114480511A (en) | 2022-05-13 |
Family
ID=81485587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210206642.1A Pending CN114480511A (en) | 2022-03-03 | 2022-03-03 | Method for preparing methane by utilizing straw anaerobic fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114480511A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2804738A1 (en) * | 2009-07-08 | 2011-01-13 | Moustafa Ahmed El-Shafie | Method and system for processing a biomass for producing biofuels and other products |
| US20110020884A1 (en) * | 2008-02-27 | 2011-01-27 | William Greg Latouf | Method for the conversion of plant materials into fuels and chemicals by sequential action of two microorganisms |
| CN102344303A (en) * | 2011-07-01 | 2012-02-08 | 辽宁省微生物科学研究院 | Microbial straw composing agent and preparation method thereof |
| CN102925494A (en) * | 2012-11-22 | 2013-02-13 | 苏州市吴江区农业环境监测站 | Method for producing methane by dry fermentation of beast and bird wastes and crop straws |
| CN104805125A (en) * | 2015-05-07 | 2015-07-29 | 马力通 | Marsh gas engineering inoculum and preparation method thereof |
| CN107279464A (en) * | 2017-08-10 | 2017-10-24 | 成都钧乔农业科技开发有限公司 | A kind of technique for being engineered fermentation of biomass feed |
| WO2019132423A1 (en) * | 2017-12-29 | 2019-07-04 | 한국전력공사 | High-purity methane production system and production method which use two-phase anaerobic fermentation |
| CN112010683A (en) * | 2020-08-18 | 2020-12-01 | 中农新科(苏州)有机循环研究院有限公司 | Rapid biological drying-aerobic fermentation treatment method for organic solid waste |
-
2022
- 2022-03-03 CN CN202210206642.1A patent/CN114480511A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110020884A1 (en) * | 2008-02-27 | 2011-01-27 | William Greg Latouf | Method for the conversion of plant materials into fuels and chemicals by sequential action of two microorganisms |
| CA2804738A1 (en) * | 2009-07-08 | 2011-01-13 | Moustafa Ahmed El-Shafie | Method and system for processing a biomass for producing biofuels and other products |
| CN102344303A (en) * | 2011-07-01 | 2012-02-08 | 辽宁省微生物科学研究院 | Microbial straw composing agent and preparation method thereof |
| CN102925494A (en) * | 2012-11-22 | 2013-02-13 | 苏州市吴江区农业环境监测站 | Method for producing methane by dry fermentation of beast and bird wastes and crop straws |
| CN104805125A (en) * | 2015-05-07 | 2015-07-29 | 马力通 | Marsh gas engineering inoculum and preparation method thereof |
| CN107279464A (en) * | 2017-08-10 | 2017-10-24 | 成都钧乔农业科技开发有限公司 | A kind of technique for being engineered fermentation of biomass feed |
| WO2019132423A1 (en) * | 2017-12-29 | 2019-07-04 | 한국전력공사 | High-purity methane production system and production method which use two-phase anaerobic fermentation |
| CN112010683A (en) * | 2020-08-18 | 2020-12-01 | 中农新科(苏州)有机循环研究院有限公司 | Rapid biological drying-aerobic fermentation treatment method for organic solid waste |
Non-Patent Citations (2)
| Title |
|---|
| 张宁: "高效厌氧纤维素降解菌复合菌系的构建及应用研究" * |
| 黄开明: "复合微生物预处理玉米秸秆提高其厌氧消化产甲烷性能" * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104630292A (en) | Method for preparing butyric acid by fermenting lignocellulose by using mixed flora | |
| CN105132469B (en) | A kind of method using lignocellulosic production biogas | |
| CN107937382B (en) | Preparation method of immobilized microalgae | |
| CN109097417B (en) | Whole-bacterium saccharification method for improving lignocellulose saccharification efficiency | |
| CN111517860A (en) | Plant nutrient rich in seaweed active oligosaccharide and preparation method thereof | |
| CN101289680A (en) | Process for producing 2,3-butanediol using american artichoke as raw material by fermentation | |
| CN106811438A (en) | A kind of straw degradative acidifying microbial inoculum and preparation method thereof | |
| CN115029393A (en) | DES additive-based method for producing medium-chain fatty acid from straw | |
| CN101864363A (en) | Complex bacterial preparation and application thereof | |
| CN111304183A (en) | Fermentation method of cellulase | |
| CN1810734A (en) | Refuse composting process adding different decay promoting ferments separately and its decay promoting ferments | |
| CN112195134A (en) | Cellulose degradation complex microbial inoculum and preparation method and application thereof | |
| CN112457134A (en) | Tree root fermented fertilizer for improving soil property and preparation method thereof | |
| CN105062928B (en) | A kind of zymomonas mobilis and its application of resisting high-concentration acetic acid and high concentration furtural | |
| CN107488680A (en) | A kind of method that biological methane gas is prepared with carbon raw material | |
| CN114480511A (en) | Method for preparing methane by utilizing straw anaerobic fermentation | |
| CN114807269B (en) | Lignocellulose whole-cell saccharification technology adopting oxygen treatment method | |
| CN1326810C (en) | Decay promoting ferment and its application in composting agricultural waste | |
| CN112795597B (en) | Method for promoting straw rapid hydrolysis by domesticating paddy soil | |
| CN102212484B (en) | Method for controlling growth morphology in fermentation process of filamentous fungi | |
| CN107647086A (en) | Method for producing microbial feed additive by adopting multi-strain mixed culture | |
| CN103343151A (en) | Preparation method of liquid medium for bacterial cellulose film | |
| CN112195197A (en) | Method for producing acid by anaerobic fermentation of sludge | |
| CN112646843B (en) | Method for preparing lactic acid by using water hyacinth as raw material | |
| CN115786125B (en) | Chlorella with high tolerance to ammonia and phenols, application and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220513 |