CN114480217B - Streptomyces strain and application thereof in inhibiting pathogenic bacteria of rice - Google Patents

Abstract

The invention discloses streptomycete and application thereof in inhibiting pathogenic bacteria of rice. The streptomycete provided by the invention is streptomycete (Streptomyces sp.) F-6, and the preservation number of the streptomycete in the China general microbiological culture Collection center is CGMCC No.22838. Experiments prove that the streptomyces sp.F-6 can inhibit bacterial leaf blight of rice, rice stripe rot and/or staphylococcus aureus, and can be used for preventing and treating bacterial leaf blight of rice and bacterial stripe rot of rice. The invention has important application value.

Description

Streptomyces strain and application thereof in inhibiting pathogenic bacteria of rice

Technical Field

The invention belongs to the field of agricultural disease control, and particularly relates to streptomycete and application thereof in inhibiting pathogenic bacteria of rice.

Background

The control of plant diseases in production mainly depends on disease-resistant varieties and chemical pesticides. Chemical pesticides are the most commonly used method for preventing and treating agricultural diseases, but the problems of environmental pollution, pesticide residues, food safety and the like caused by long-term use of chemical pesticides are increasingly prominent. Plant pathogens are affected by environmental conditions and by host plants during growth and development, and methods for controlling disease development using beneficial microorganisms are called plant disease biocontrol, which are called plant disease biocontrol bacteria. Biological control is a research hot spot for controlling plant soil-borne diseases at home and abroad at present because of the characteristics of low cost, wide sources, environmental friendliness, long-term benefit and the like. Screening and constructing biocontrol strain with comprehensive excellent performance and developing biocontrol microbial inoculum have become an important development direction for biological control of plant diseases.

At present, biocontrol bacteria widely applied in production include fungi, actinomycetes and the like. The fungi mainly comprise trichoderma, chaetomium, saccharomycetes, paecilomyces lilacinus, verticillium pachyrhizus, mycorrhizal fungi and the like; actinomycetes mainly include Streptomyces and variants thereof. The actinomycetes have the characteristics of good control effect, wide action spectrum, strong biological diversity and rich types of antibiotics, and are widely applied to plant disease control, such as jinggangmycin, endotheracin, 768, S921, agricultural resistance 120 and the like which are developed by the actinomycetes and can be used for controlling poplar rot pathogens, botrytis cinerea, cucumber cladosporium cucumerinum, pepper phytophthora capsici, cotton verticillium, rhizoctonia solani, tomato early blight pathogens, gray mold pathogens, cabbage black rot pathogens, cabbage soft rot pathogens and the like. A great deal of researches show that a plurality of biocontrol microorganisms can secrete a plurality of plant hormone substances or other growth promoting factors while antagonizing plant pathogenic microorganisms, and can enhance the disease resistance of plants and indirectly control the occurrence of plant diseases in the process of promoting the growth of the plants.

Disclosure of Invention

The invention aims to inhibit germs such as rice bacterial leaf blight germs, rice stripe spot germs and staphylococcus aureus.

The invention firstly protects a strain of Streptomyces, namely Streptomyces F-6, which is preserved in China general microbiological culture collection center (CGMCC for short, address: north Chen West Lu No. 1, 3 of Beijing Korea, the region of towards the sun) in 2021, month 07 and day 07, and the preservation number is CGMCC No.22838. Streptomyces sp. F-6CGMCC No.22838 is simply referred to as Streptomyces F-6.

The invention also provides a microbial inoculum which can contain streptomycete F-6. The use of the microbial inoculum may be at least one of the following a 1) to a 6):

a1 Inhibiting bacterial leaf blight of rice;

a2 Preparing a product for inhibiting bacterial leaf blight of rice;

a3 Inhibiting rice stripe bacteria;

a4 Preparing a product for inhibiting rice stripe bacteria;

a5 Inhibition of staphylococcus aureus;

a6 A product that inhibits staphylococcus aureus.

The invention also provides a preparation method of the microbial inoculum, which can comprise the following steps: and inoculating streptomycete F-6 into a bacterial culture medium, and culturing to obtain bacterial liquid, namely the bacterial agent.

The bacterial culture medium may be a high-k No. 1 liquid culture medium.

In addition to the active ingredient, the microbial agent may also include a carrier. The carrier may be a solid carrier or a liquid carrier. The solid support may be a mineral material, a plant material or a polymeric compound. The mineral material may be at least one of clay, talc, kaolin, montmorillonite, white carbon, zeolite, silica, and diatomaceous earth. The plant material may be at least one of bran, soybean meal, corn flour, bean flour and starch. The polymer compound may be polyvinyl alcohol and/or polyglycol. The liquid carrier may be an organic solvent, vegetable oil, mineral oil, or water. The organic solvent may be decane and/or dodecane. In the microbial inoculum, the active ingredient may be present in the form of living cells being cultured, a fermentation broth of living cells, a filtrate of a cell culture, or a mixture of cells and filtrate. The formulation of the composition can be various formulations, such as liquid, emulsion, suspending agent, powder, granule, wettable powder or water dispersible granule.

Surfactants (such as Tween 20, tween 80, etc.), binders, stabilizers (such as antioxidants), pH regulators, etc. can also be added into the microbial inoculum according to the need.

The invention also protects streptomyces F-6 or the application of the microbial inoculum, which can be at least one of the following a 1) to a 6):

a1 Inhibiting bacterial leaf blight of rice;

a2 Preparing a product for inhibiting bacterial leaf blight of rice;

a3 Inhibiting rice stripe bacteria;

a4 Preparing a product for inhibiting rice stripe bacteria;

a5 Inhibition of staphylococcus aureus;

a6 A product that inhibits staphylococcus aureus.

The invention also protects the application of the streptomycete F-6 or any of the microbial inoculum in preventing and treating diseases caused by rice bacterial blight.

The invention also protects the application of the streptomycete F-6 or any one of the microbial inoculum in preventing and controlling diseases caused by rice stripe bacteria.

The invention also protects the streptomycete F-6 or the application of any one of the microbial inoculum in preventing and treating diseases or diseases caused by staphylococcus aureus.

The invention also protects a product A which contains streptomycete F-6 or any one of the microbial agents; the function of the product a may be a 1) or a 3) or a 5):

a1 Inhibiting bacterial leaf blight of rice;

a3 Inhibiting rice stripe bacteria;

a5 Inhibit staphylococcus aureus.

The invention also protects a product B which contains streptomycete F-6 or any one of the microbial inoculum; the function of the product b may be b 1) or b 2) or b 3):

b1 Preventing and treating diseases caused by bacterial leaf blight of rice;

b2 Preventing and treating diseases caused by rice stripe bacteria;

b3 Preventing and treating diseases or diseases caused by staphylococcus aureus.

Any of the diseases caused by the bacterial leaf blight of rice can be bacterial leaf blight of rice;

any of the above-mentioned diseases caused by rice leaf spot bacteria may be rice bacterial leaf spot.

The invention also provides a method for inhibiting bacteria, which can treat substances containing bacteria by adopting streptomyces F-6.

In the above method, the "treatment of a pathogen-containing substance with Streptomyces F-6" can be achieved by adding a pathogen-containing substance to the fermentation broth of Streptomyces F-6. The germ can be at least one of rice bacterial leaf blight germ, rice stripe spot germ and staphylococcus aureus.

Experiments prove that the Streptomyces sp.F-6 can inhibit bacterial leaf blight of rice, rice stripe rot and/or staphylococcus aureus, and can be used for preventing and treating bacterial leaf blight of rice and bacterial stripe rot of rice. The invention has important application value.

Preservation description

Strain name: streptomyces sp

Latin name: streptomyces sp.

Strain number: f-6

Preservation mechanism: china general microbiological culture Collection center (China Committee for culture Collection of microorganisms)

The preservation organization is abbreviated as: CGMCC

Address: beijing city, chaoyang area, north Chenxi Lu No. 1 and 3

Preservation date: 2021, 07

Accession numbers of the preservation center: CGMCC No.22838

Drawings

FIG. 1 shows the growth state of 2 colonies in step one of example 1.

FIG. 2 is a phylogenetic tree of step two actinomycetes F-6 in example 1.

FIG. 3 is a growth curve of Streptomyces F-6 of example 2.

FIG. 4 shows the inhibitory effect of Streptomyces F-6 on Rhizoctonia solani, rhizoctonia cerealis and Staphylococcus aureus in example 3.

Detailed Description

The following detailed description of the invention is provided in connection with the accompanying drawings that are presented to illustrate the invention and not to limit the scope thereof. The examples provided below are intended as guidelines for further modifications by one of ordinary skill in the art and are not to be construed as limiting the invention in any way.

The experimental methods in the following examples, unless otherwise specified, are conventional methods, and are carried out according to techniques or conditions described in the literature in the field or according to the product specifications. Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.

MH broth was obtained from Qingdao Gaojingyuan Haibo Biotechnology Co., ltd and was designated HB6231.

MH broth solid medium: 6g of beef extract powder, 1.5g of soluble starch and 17.5g of acid hydrolyzed casein are dissolved in a proper amount of distilled water, and then 20g of agar (product number: A6190 of Beijing Bo Ottoda technology Co., ltd.) is added, and distilled water is used for constant volume to 1L, and sterilization is carried out for 15min at 121 ℃.

The liquid culture medium of Gaoshi No. 1 is a product of Qingdao Gaokou Haibo biotechnology Co., ltd, and the product number is HB8550-1.

Solid medium No. 1 gao: dissolving soluble starch 20g and KNO ₃ 1g、K ₂ HPO ₄ 0.5g、MgSO ₄ ·7H ₂ O0.5g, naCl 0.5g and FeSO ₄ ·7H ₂ Dissolving O0.01 g in appropriate amount of distilled water, adding agar 20g, diluting to 1L with distilled water, adjusting pH to 7.4-7.6, and sterilizing for 15min.

Bacterial leaf blight bacteria (Xanthomonas oryzae. Oryzae), rice stripe bacteria (Xanthomonas oryzae. Oryzicola) and staphylococcus aureus (Staphylococcus aureus) are all provided by the university of Ningxia medical microbiology and Biochemical pharmaceutical laboratory.

Soil samples in the examples below were taken from the western medlar institute of the Ningxia Hui autonomous area.

EXAMPLE 1 isolation, identification and preservation of Streptomyces sp F-6CGMCC No.22838, isolation

1. Adding 1g of soil sample and appropriate amount of glass beads into 9mL of sterile water, oscillating for 10min, standing for 30s to obtain soil stock solution (dilution is 10 at this time) ^-1 ). The supernatant of 1mL of the stock solution was added to a test tube containing 9mL of sterile water and thoroughly mixed (at a dilution of 10) ^-2 ) Then 1mL of the solution was taken out of the tube and added to another tube containing 9mL of sterile water for mixing uniformly, and so on to prepare a diluent (dilution of 10 ^-4 )。

2. 100. Mu.L of the dilutions were respectively aspirated by a pipette and spread evenly on Gaoshi No. 1 solid medium, incubated at 28℃for 5-7d, and the growth state of the colonies was observed.

The growth state of the colonies is shown in FIG. 1.

3. After the step 2 is completed, each colony is inoculated into a conical flask containing 150mL of Gaoshi No. 1 liquid culture medium, and the culture is carried out on a shaking table in a 28-shaped manner and 180r/min to obtain corresponding fermentation broth.

4. Inoculating rice stripe bacteria to MH broth solid culture medium, and standing at 37deg.C for 24 hr; then adding 0.2mL of sterile water to the MH broth solid culture medium, scraping the rice leaf spot bacteria by using a sterile inoculation shovel to obtain a rice leaf spot bacteria suspension (the concentration of the rice leaf spot bacteria is about 10) ⁸ CFU/mL)。

5. And (3) uniformly coating 50 mu L of the rice leaf spot germ suspension obtained in the step (4) on the MH broth solid culture medium, placing filter paper sheets, respectively dripping 10 mu L of the fermentation liquor obtained in the step (3) on each filter paper sheet, and culturing for 5-7d at 28 ℃. Colonies that can form a zone of inhibition were observed. Bacterial colony capable of forming bacteria inhibition zone is subjected to bacterial purification in Gaoshi No. 1 solid culture medium.

The selected actinomycetes were designated actinomycetes F-6.

2. Authentication

1. Morphological identification

Actinomycetes F-6 were inoculated onto a solid medium of Gao's No. 1, cultured at 28℃for 5-7d, and the state of single colony was observed.

The results show that the colony of actinomycetes F-6 is purple, compact in texture, firm in surface, dry, small and irregularly round, and gradually appears dark purple brown after culture, and the culture medium is brown.

2. Molecular characterization

The 16S rDNA of actinomycetes F-6 is shown as SEQ ID NO. 1.

The sequences shown in SEQ ID NO. 1 are subjected to homology comparison and analysis, strains with high homology and actinomycetes F-6 are selected for phylogenetic analysis, a phylogenetic tree is constructed by a Neighbor-Joining method (Neighbor-Joining) in MEGA software, and finally the genus of actinomycetes F-6 is determined.

The results are shown in FIG. 2. Actinomycetes F-6 are the closest genetic distance to Streptomyces sp.

Actinomycetes F-6 were identified as Streptomyces sp according to their morphological features, molecular identification and Streptomyces (Streptomyces) seed separation table.

3. Preserving

The actinomycetes F-6 separated in the first step are preserved in China general microbiological culture Collection center (CGMCC) in the 07 th month of 2021, and the preservation number is CGMCC No.22838. The actinomycetes F-6 are all called Streptomyces F-6CGMCC No.22838, which is called Streptomyces F-6 for short.

Example 2 determination of the growth curve of Streptomyces F-6

1. The single colony of streptomycete F-6 is inoculated to 5mL of Gao's No. 1 liquid culture medium, and cultured for 2d at 28 ℃ and 180r/min to obtain seed liquid.

2. 3mL of the seed solution was inoculated into a flask (250 mL in size) containing 150mL of liquid medium of Gaoshi No. 1 (the inoculum size was 2%), and cultured at 28℃for 15d at 180 r/min. From 48h (2 d) of culture, filtering the culture solution with quantitative filter paper every 24h to obtain mycelium; and washing the mycelium, and drying at 60 ℃ to constant weight to obtain the dry weight of the mycelium. Each time node 3 groups were in parallel and averaged.

And drawing a growth curve by taking the culture time as an abscissa and the dry weight of mycelium as an ordinate.

The growth curve of Streptomyces F-6 is shown in FIG. 3. The results show that in medium No. 1 of Gao, streptomyces F-6 grows slowly at 2-7d, increases in multiples after 7d, and gradually flattens after 11 d.

EXAMPLE 3 bacteriostatic Effect of Streptomyces F-6

1. Inhibition effect of streptomyces F-6 on rice stripe bacteria

Experiments were repeated three times and the average was taken. The specific steps of each experiment are as follows:

1. inoculating single colony of streptomycete F-6 into 150mL of Gao's No. 1 liquid culture medium, culturing at 28 ℃ for 7d at 180r/min, and filtering; then adsorbing with C18 adsorption column, eluting with methanol, and air drying; finally, the Streptomyces fermentation broth is dissolved by DMSO to obtain a sample with the concentration of about 25.0mg/mL.

2. Inoculating single bacterial colony of rice stripe bacteria to 5mLMH broth liquid culture medium, and shake culturing at 37deg.C and 180r/min for 24 hr to obtain bacterial liquid.

3. After the step 2 is completed, MH broth liquid culture medium is added into 1mL of bacterial liquid for dilution, thus obtaining bacterial suspension of rice stripe bacteria (the concentration of the rice stripe bacteria is about 10) ⁸ CFU/mL)。

4. After the step 3 is completed, 100 mu L of rice stripe germ suspension is taken and evenly coated on MH broth solid culture medium.

5. After the completion of step 4, the MH broth solid medium was uniformly divided into five aliquots, sterile filter paper having a diameter of 8mm was placed in the center of each aliquot, and then 10. Mu.L DMSO (as a blank control), 10. Mu.L streptomycin (2.5. Mu.g/mL as a positive control), 10. Mu.L of the Streptomyces fermentation broth obtained in step 1, and 10. Mu.L of the Streptomyces fermentation broth obtained in step 1 were inoculated on the sterile filter paper, and the culture was allowed to stand at 37℃for 24 hours to observe the bacteriostatic effect.

The partial bacteriostatic effect of streptomyces F-6 on rice stripe bacteria is shown in figure 4A (Streptomycin is Streptomycin and F-6 is streptomyces fermentation broth). The result shows that after the fermentation broth of the streptomyces punctatus is cultured, a bacteriostasis ring appears (the average diameter of the bacteriostasis ring is about 22.12 mm); after the spot-grafting DMSO culture, no inhibition zone appears; after the culture of the spot-jointed streptomycin (used as a positive control), a zone of inhibition appears (the average diameter of the zone of inhibition is about 23.67 mm).

Therefore, the streptomyces F-6 has remarkable inhibition effect on rice stripe bacteria, and the inhibition effect is close to that of streptomycin.

2. Inhibition effect of streptomycete F-6 on rice bacterial leaf blight bacteria

According to the method of the first step, the rice stripe bacteria are replaced by rice bacterial leaf blight bacteria, and other steps are unchanged.

The partial bacteriostatic effect of streptomyces F-6 on rice bacterial blight is shown in figure 4B (Streptomycin is Streptomycin, and F-6 is streptomyces fermentation broth). The results show that after the culture of the inoculated streptomycete fermentation broth, there is an appearance of a bacteriostasis zone (the average diameter of the bacteriostasis zone is about 22.45mm, after the culture of the inoculated streptomycete fermentation broth, there is no appearance of the bacteriostasis zone, and after the culture of the inoculated streptomycete fermentation broth, the inoculated streptomycete fermentation broth is used as a positive control, there is an appearance of the bacteriostasis zone (the average diameter of the bacteriostasis zone is about 23.17 mm).

Therefore, the streptomycete F-6 has remarkable inhibiting effect on rice bacterial leaf blight bacteria.

3. Inhibition effect of streptomycete F-6 on staphylococcus aureus

According to the method of the first step, the rice stripe bacteria are replaced by staphylococcus aureus, the streptomycin serving as a positive control is replaced by penicillin, and other steps are unchanged.

The partial bacteriostatic effect of Streptomyces F-6 on Staphylococcus aureus is shown in FIG. 4C (Penicilli is penicillin and F-6 is Streptomyces fermentation broth). The result shows that after the fermentation broth of the streptomyces punctatus is cultured, a bacteriostasis ring appears (the average diameter of the bacteriostasis ring is about 51.05 mm); after the spot-grafting DMSO culture, no inhibition zone appears; after the cultivation of the spot-jointed penicillin, a zone of inhibition appears (the average diameter of the zone of inhibition is about 21.16 mm).

Therefore, the streptomycete F-6 has remarkable inhibiting effect on staphylococcus aureus.

Example 4 detection of minimum inhibitory concentration of Streptomyces F-6 fermentation product against Rhizoctonia solani, rhizoctonia cerealis and Staphylococcus aureus

The bacteria to be detected are rice bacterial leaf blight bacteria, rice stripe bacteria or staphylococcus aureus.

1. Inoculating single colony of streptomycete F-6 into 150mL of Gao's No. 1 liquid culture medium, culturing at 28 ℃ for 7d at 180r/min, and filtering; and then adsorbing by using a C18 adsorption column, eluting with methanol, and airing to obtain a streptomycete F-6 fermentation product.

2. 96-well plates (8 rows of 12 wells) were taken, with 180. Mu.LMH broth added to the 1 st well of each row, 100. Mu.LMH broth added to the 2 nd well-11 th well, and 200. Mu.LMH broth added to the 12 th well.

3. After the step 2 is completed, 20 mu L of streptomycete F-6 fermentation product is added into the 1 st hole, uniformly mixed, and 100 mu L of the fermentation product is sucked by a pipette and added into the 2 nd hole; mixing the liquid in the 2 nd hole uniformly, sucking 100 mu L of the liquid into the 3 rd hole by using a liquid-transferring gun; and so on, up to the 10 th well. The 10 th well was mixed, 100. Mu.L was pipetted and discarded.

At this time, the liquid in each of wells 1 to 11 was 100. Mu.L. The liquid in the 12 th well was 200. Mu.L.

Wherein the liquid in the 1 st well-10 th well contains different concentrations of Streptomyces F-6 fermentation products. The concentration of Streptomyces F-6 fermentation product in wells 1 through 10 was examined to be 100.00, 50.00, 25.00, 12.50, 6.25, 3.12, 1.56, 0.78, 0.39 and 0.19mg/mL in this order.

4. After the step 3 is completed, 100 mu L of bacterial suspension to be detected is inoculated into the 1 st hole-11 th hole, the mixture is uniformly mixed, the mixture is cultured for 24 hours at 37 ℃, the lowest concentration for completely inhibiting the growth of bacteria in the small hole is taken as the lowest antibacterial concentration, and the result is observed and recorded.

The preparation method of the bacterial suspension to be tested comprises the following steps: inoculating single bacterial colonies to be detected to a 5mLMH broth liquid culture medium, and performing shake culture at 37 ℃ and 180r/min for 24 hours to obtain bacterial liquid; then adding MH broth into 1mL of bacterial liquid for dilution to obtain bacterial suspension of the bacteria to be detected (the concentration of the bacteria to be detected is about 10) ⁸ CFU/mL)。

The result shows that the minimum concentration of the streptomyces F-6 fermentation product capable of inhibiting rice stripe bacteria is 3.12mg/mL, the minimum concentration capable of inhibiting rice bacterial leaf blight bacteria is 3.12mg/mL, and the minimum concentration capable of inhibiting staphylococcus aureus is 0.78mg/mL. The present invention is described in detail above. It will be apparent to those skilled in the art that the present invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with respect to specific embodiments, it will be appreciated that the invention may be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The application of some of the basic features may be done in accordance with the scope of the claims that follow.

<110> Ningxia medical university Ningxia agricultural and forestry academy of sciences plant protection institute (Ningxia plant disease and pest control key laboratory)

<120> Streptomyces strain and use thereof in inhibiting pathogenic bacteria of rice

<160>1

<170> PatentIn version 3.5

<210>1

<211>1041

<212>DNA

<213>Artificial sequence

<400>1

gggcgtgcgc gtgcttacca tgcaagtcga acgatgaacc acttcggtgg ggattagtgg 60

cgaacgggtg agtaacacgt gggcaatctg cccttcactc tgggacaagc cctggaaacg 120

gggtctaata ccggatacca ctaccgcagg catctgtggt ggttgaaagc tccggcggtg 180

aaggatgagc ccgcggccta tcagcttgtt ggtgaggtaa tggctcacca aggcgacgac 240

gggtagccgg cctgagaggg cgaccggcca cactgggact gagacacggc ccagactcct 300

acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcgacgccgc 360

gtgagggatg acggccttcg ggttgtaaac ctctttcagc agggaagaag cgaaagtgac 420

ggtacctgca gaagaagcgc cggctaacta cgtgccagca gccgcggtaa tacgtagggc 480

gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt cacgtcgggt 540

gtgaaagccc ggggcttaac cccgggtctg cattcgatac gggctagcta gagtgtggta 600

ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag gaacaccggt 660

ggcgaaggcg gatctctggg ccattactga cgctgaggag cgaaagcgtg gggagcgaac 720

aggattagat accctggtag tccacgccgt aaacggtggg aactaggtgt tggcgacatt 780

ccacgtcgtc ggtgccgcag ctaacgcatt aagttccccg cctggggagt acggccgcaa 840

ggctaaaact caaaggaatt gacgggggcc cgcacaagca gcggagcatg tggcttattt 900

cgacgcaacg cgaagaacct taccaaggct tgacatacac cggaaacggc cagagatggt 960

cgcccccttg tggtcggtgt acaggtggtg catggctgtc gtcagcctcg tgtcgtgaga 1020

tgttgggtta gtcccgcaac g 1041

Claims (10)

1. Streptomyces sp.F-6 has a collection number of CGMCC No.22838 in the China general microbiological culture Collection center.

2. A microbial inoculum comprising the Streptomyces sp. F-6 according to claim 1.

3. The method for preparing the microbial inoculum of claim 2, comprising the following steps: inoculating the streptomyces sp. F-6 of claim 1 to a bacterial culture medium and culturing to obtain a bacterial liquid, namely a bacterial agent.

4. Use of a streptomyces sp. F-6 according to claim 1 or a microbial agent according to claim 2, being at least one of the following a 1) -a 5):

a1 Inhibition of rice bacterial leaf blight bacteria xanthomonas oryzae.

a2 Preparing a product for inhibiting bacterial blight bacteria xanthomonas oryzae.

a3 Inhibiting Xanthomonas oryzae.

a4 Preparing a product for inhibiting rice stripe bacteria Xanthomonas oryzae.

a5 A product that inhibits staphylococcus aureus.

5. Use of the microbial inoculum according to claim 1 of streptomyces F-6 or claim 2 for controlling diseases caused by the bacterial strain xanthomonas oryzae.

6. Use of a streptomyces sp. F-6 according to claim 1 or a microbial inoculum according to claim 2 for controlling diseases caused by the species fusarium graminearzaepv.

7. Use of a streptomyces sp. F-6 according to claim 1 or a microbial inoculum according to claim 2 for the preparation of a product for controlling diseases or disorders caused by staphylococcus aureus.

8. A product a comprising the streptomyces F-6 of claim 1 or the microbial agent of claim 2; the function of the product A is A1) or A2) or A3):

a1 Inhibition of rice bacterial leaf blight bacteria xanthomonas oryzae.

A2 Inhibiting Xanthomonas oryzae.

A3 Inhibit staphylococcus aureus.

9. A product b comprising the streptomyces F-6 of claim 1 or the microbial agent of claim 2; the function of the product B is b 1) or b 2) or b 3):

b1 Prevention and treatment of diseases caused by rice bacterial leaf blight bacteria xanthomonas oryzae.

b2 Prevention and treatment of diseases caused by rice stripe bacteria Xanthomonas oryzae v.

b3 Preventing and treating diseases or diseases caused by staphylococcus aureus.

10. A method for inhibiting a pathogen, comprising treating a pathogen-containing substance with the Streptomyces sp. F-6 strain of claim 1; the germ is xanthomonas oryzae or xanthomonas oryzae.

Images