CN114478798A - Protein or protein composition, and preparation method and application thereof - Google Patents

Protein or protein composition, and preparation method and application thereof Download PDF

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CN114478798A
CN114478798A CN202210055493.3A CN202210055493A CN114478798A CN 114478798 A CN114478798 A CN 114478798A CN 202210055493 A CN202210055493 A CN 202210055493A CN 114478798 A CN114478798 A CN 114478798A
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ser
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asn
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晏巧玲
李荩
莘春林
朱涛
齐源远
吴丹
李洪艳
王仕成
赵换
隋秀文
苗伟
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Kangxinuo Biological Technology Shanghai Co ltd
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CanSino Biologics Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
    • C12N2770/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
    • C12N2770/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

Abstract

The present invention provides an mRNA and a composition comprising the mRNA, wherein the mRNA comprises an mRNA sequence encoding a SARS-2-CoV coronavirus S protein or a variant thereof. The invention also provides a protein and a composition containing the protein, wherein the protein comprises a variant or derivative of SARS-2-CoV type coronavirus S protein or a fragment thereof. The invention also provides the application of the mRNA or the composition and the protein or the composition in preparing the medicine (especially the vaccine) for preventing and/or treating the SARS-2-CoV type coronavirus infection.

Description

Protein or protein composition, and preparation method and application thereof
Technical Field
The invention relates to the technical field of vaccine development, in particular to mRNA containing a coding region for coding SARS-2-CoV coronavirus S protein or a variant thereof and a composition thereof, protein containing the variant or a derivative of the SARS-2-CoV coronavirus S protein or a fragment thereof and a composition thereof, and application of the mRNA, the composition or the protein and the composition thereof in preparing a medicament (especially a vaccine) for preventing and/or treating SARS-2-CoV coronavirus infection.
Background
Coronaviruses are nonsegmented, single-stranded, positive-strand RNA viruses belonging to the positive-coronavirus subfamily (orthocoronaviridae) of the nidus (Nidovirales) and are classified into four genera, α, β, γ, and δ, according to serotype and genomic characteristics. To date, a total of 7 coronaviruses can infect humans: including 229E and NL63 of the alpha genus, OC43 and HKU1 of the beta genus, middle east respiratory syndrome-associated coronavirus (MERSR-CoV), Severe acute respiratory syndrome-associated coronavirus (SARSr-CoV), and novel coronavirus (SARS-2-CoV). Only the latter three of them can lead to serious human illness and even death.
Coronaviruses are enveloped, and particles are round or oval, often polymorphic, and typically 50-200 nm in diameter. The S protein is located on the surface of the virus to form a rod-shaped structure, and is one of main antigen proteins of the virus, and is a main gene for typing. The N protein wraps the viral genome and can be used as a diagnostic antigen.
The understanding of the physicochemical properties of coronaviruses is mostly derived from the study of SARS-CoV and MERS-CoV. Vaccines designed based on the full-length S protein of SARS-CoV are reported to induce a large amount of non-neutralizing antibodies, fail in toxicity attack in animal models and cause severe side effects such as increased morbidity, strong inflammatory response and liver damage in liver tissues ("Evaluation of modified vaccinia virus and kara based recombinant SARS Vaccine in vaccines" 23, 2273-2279 "). Therefore, avoiding exposure of non-neutralizing epitopes with immune dominance in vaccine design is the basis for ensuring vaccine safety. Meanwhile, studies on the S proteins of SARS-CoV and MERS-CoV revealed that substitution of 2 prolines in the loop region between the first heptad repeat (HR1) and the central helical region could fix the S protein in the prefusion conformation and increase the expression yield of the extracellular region of the S protein ("Immunogenicity and structure of a ratioingly designed prefusion MERS-CoV spike antigen", Proc Natl Acad Sci US A.114(35): E7348-E7357.).
The Receptor Binding Domain (RBD) is the region where the neutralizing epitope is most concentrated. The research result of the S protein structure of SARS-CoV shows that only 3% of 3 RBDs on the S protein trimer are in an open state, namely in a contact state; 39% of the trimer was 2 RBDs open, 1 RBD closed; only 1 RBD of 58% of the trimers was in the open state; after S1 and S2 are separated, the state of the RBD is more closed, and only 0.3 percent of all RBDs of the trimer are in an open state; and 4.1% of proteins with RBDs in a closed state ("Stabilized mammalian species resistor to transformed change induced by receptor homology or protein", Sci Rep.24; 8(1):15701.) appear.
Therefore, the present invention provides an mRNA vaccine and a subunit vaccine that avoids the immunodominant non-neutralizing epitopes while leaving the RBD more open for the induction of neutralizing antibodies.
Disclosure of Invention
The invention provides an optimized design aiming at SARS-2-CoV type coronavirus spike protein, which introduces at least one mutation in the specific site of the amino acid sequence of the spike protein to change the S protein conformation, improve the level of immune production neutralizing antibody and reduce the level of ADE effect antibody. The design can be applied to recombinant protein vaccines, mRNA vaccines, DNA vaccines and other vaccines, and is used for preventing and/or treating coronavirus infection.
In a first aspect of the invention, there is provided an mRNA comprising an mRNA sequence encoding a variant of a SARS-2-CoV-type coronavirus S protein, or fragment thereof, said variant of the S protein comprising the amino acid sequence of SEQ ID NO:26, and a variant of said S protein fragment comprising SEQ ID NO:27, and said SEQ ID NO:26 or SEQ ID NO:27 comprises any one or a combination of two or more of the following:
A) mutation at L611, preferably, L611 to a611 or G611;
B) mutation at position Y612, preferably mutation Y612 to a612 or G612;
C) a mutation at position Q613, preferably Q613 to a613 or G613;
D) a mutation at position D614, preferably, the mutation of D614 is a614 or G614;
E) mutation at position T859, preferably, the T859 mutation is a859 or G859.
Wherein the variant of S protein or the variant of S protein fragment or the mRNA encoding the variant of S protein or the variant of S protein fragment is capable of eliciting an immune response in a human which results in the production of neutralizing antibodies.
Meanwhile, some of the disclosures in the prior art also support the technical solutions of the present invention, for example, it was found that mAbs mAb 43-3-14 can enhance Infection of Vero E6 cells by SARS-CoV, and exhibit disease-Enhancing ability, while L597, Y598, Q599, D600 and C603 play a key role in the recognition of S protein by mAb 43-3-14 (see Qidi Wang et al (2016), Immunodominant SARS Coronavir genes Epitopes in human electronics enhanced and neutral Effects in Non-human principles, ACS, Infection. Dis.2016,2, 361-). Furthermore, the mutation of D614G in SARS-CoV-2 can increase the proportion of S trimer in fully open state of RBD from undetectable to 20%, presumably because the mutation of D614 disrupts the hydrogen bond with T859 (see Leonid Yurkovitskiy et al (2020), Structural and Functional Analysis of the D614G SARS-CoV-2Spike Protein Variant, Cell 183, 1-13.).
Preferably, the nucleotide sequence of SEQ ID NO:26 or SEQ ID NO: the mutation of 27 further comprises a mutation at position 682RRAR685 and/or a mutation at position 986KV987 to fix the S protein to the prefusion conformation. Further preferred, 682RRAR685 and/or 986KV987 are mutated to GSAG and/or PP. Also, the disclosure of the prior patents supports the technical solution of the present invention, such as the very efficient stable conformation of one or two amino acids by proline in the region close to or in the first heptad repeat region (see U.S. patent application No. 20200061185, PREFUSION CORONAVIRUS SPIKE PROTEINS and THEIR USE).
Preferably, the S protein is a full-length S protein. The S protein fragment is preferably the extracellular region of the S protein.
In a specific embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of L611A in a) is shown as SEQ ID NO:1 or a variant of SEQ ID NO:1, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical; the amino acid sequence of the full-length S protein with the specific mutation site of L611A fixed in the pre-fusion conformation in the A) is shown as SEQ ID NO:2 or a variant of SEQ ID NO:2, 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
In a specific embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of Y612A in B) is shown in SEQ ID NO: 3 or a sequence identical to SEQ ID NO: 3 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the full-length S protein which has the specific mutation site of Y612A and is fixed in the pre-fusion conformation in the B) is shown as SEQ ID NO: 4 or a sequence identical to SEQ ID NO: 4, 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
In a specific embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of Q613A in C) is shown in SEQ ID NO: 5 or a sequence identical to SEQ ID NO: 5 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the full-length S protein with the specific mutation site of Q613A fixed in the pre-fusion conformation in C) is shown as SEQ ID NO: 6 or a sequence identical to SEQ ID NO: 6 amino acid sequences with 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
In a specific embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of D614G in D) is shown in SEQ ID NO: 7 or a sequence identical to SEQ ID NO: 7 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the full-length S protein which has the specific mutation site of D614G and is fixed in the pre-fusion conformation is shown as SEQ ID NO: 8 or a variant of SEQ ID NO: 8, 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
In one embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of 611LYQD614 and 611AAAG614 in the combination of a) -D) is as shown in SEQ ID NO:9 or a sequence identical to SEQ ID NO:9, amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the full-length S protein fixed in the pre-fusion conformation, of which the specific mutation site in the A) -D) combination is 611LYQD614 and 611AAAG614 is shown as SEQ ID NO: 10 or a sequence identical to SEQ ID NO: 10, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical.
In a specific embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of T859A in the E) is shown as SEQ ID NO. 11 or an amino acid sequence with 70%, 75%, 80%, 85%, 90%, 95% or 99% of identity with the SEQ ID NO. 11; the specific mutation site in E) is T859A, and the full-length S protein amino acid sequence fixed in the pre-fusion conformation is shown as SEQ ID NO. 12 or an amino acid sequence with 70%, 75%, 80%, 85%, 90%, 95% and 99% of identity with SEQ ID NO. 12.
In a specific embodiment of the invention, the amino acid sequence of the extracellular region of the wild-type S protein with the specific mutation site of L611A in a) is shown in SEQ ID NO: 13 or a sequence identical to SEQ ID NO: 13 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein with the specific mutation site of L611A in the A) and fixed in the pre-fusion conformation is shown as SEQ ID NO: 14 or a sequence identical to SEQ ID NO: 14, 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
In a specific embodiment of the invention, the amino acid sequence of the extracellular region of the wild-type S protein with the specific mutation site of Y612A in B) is shown in SEQ ID NO: 15 or a variant of SEQ ID NO: 15, amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein fixed in the pre-fusion conformation, of which the specific mutation site in B) is Y612A, is shown as SEQ ID NO: 16 or a sequence identical to SEQ ID NO: 16, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical.
In a specific embodiment of the invention, the amino acid sequence of the extracellular region of the wild-type S protein with the specific mutation site of C) being Q613A is shown in SEQ ID NO: 17 or a variant of SEQ ID NO: 17 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein with the specific mutation site of Q613A fixed in the pre-fusion conformation in C) is shown as SEQ ID NO: 18 or a sequence identical to SEQ ID NO: 18, 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
In a specific embodiment of the invention, the amino acid sequence of the extracellular region of the wild-type S protein with the specific mutation site of D614G in D) is shown in SEQ ID NO: 19 or a variant of SEQ ID NO: 19, amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein fixed in the pre-fusion conformation, the specific mutation site of which is D614G in D) is shown as SEQ ID NO: 20 or a sequence identical to SEQ ID NO: 20, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical.
In one embodiment of the invention, the amino acid sequence of the extracellular region of the wild-type S protein with the specific mutation site of 611LYQD614 mutated to 611AAAG614 in the combination of A) -D) is shown as SEQ ID NO 21 or the amino acid sequence of the extracellular region of the wild-type S protein with the specific mutation site of 611LYQD614 mutated to 611AAAG614 is shown as SEQ ID NO:21 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein which is fixed in the pre-fusion conformation and has a specific mutation site of 611LYQD614 in the A) -D) combination is shown as SEQ ID NO. 22 or an amino acid sequence which has 70%, 75%, 80%, 85%, 90%, 95% and 99% of identity with the SEQ ID NO. 22.
In a specific embodiment of the invention, the amino acid sequence of the extracellular region of the wild-type S protein with the specific mutation site of T859A in E) is as shown in SEQ ID NO: 23 or a variant of SEQ ID NO: 23, amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein fixed in the pre-fusion conformation, of which the specific mutation site in E) is T859A, is shown as SEQ ID NO: 24 or a sequence identical to SEQ ID NO: 24, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical. A protein encoded by the mRNA or composition of the invention.
Preferably, the nucleotide sequence of the mRNA is shown in SEQ ID NO:28-51 or a variant of any of SEQ ID NOs: 28-51, having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
Preferably, the mRNA further comprises a sequence encoding a signal peptide which encodes a secretion-recognizable signal peptide of a human cell, the signal peptide being selected from the group consisting of a signal peptide of SARS-2-CoV-type coronavirus S protein, a signal peptide of tissue plasminogen activator (tPA) and/or a signal peptide of serum immunoglobulin E (lgE), and more preferably a signal peptide of SARS-2-CoV-type coronavirus S protein.
Preferably, the mRNA further comprises a 5 ' cap structure, a 5 ' noncoding region, a3 ' noncoding region, and/or a polyadenylation tail mRNA sequence.
Preferably, the mRNA further comprises one or a combination of two or more of a 5 ' conserved sequence element, an RNA replicase coding region, a subgenomic promoter, a3 ' conserved sequence element, or a3 ' non-coding region.
Preferably, the mRNA further comprises a 5 ' cap structure, a 5 ' conserved sequence element, a subgenomic promoter, a3 ' conserved sequence element, and/or a poly a tail.
Preferably, the mRNA is a conventional mRNA, an autonomously amplifying mRNA or a trans-amplifying mRNA.
In a specific embodiment of the invention, the mRNA is a conventional mRNA, i.e.the mRNA comprises, in addition to the mRNA sequence encoding the S protein of the novel coronavirus SARS-CoV-2 or a variant thereof, a 5 ' cap structure, a 5 ' non-coding region, a3 ' non-coding region and/or a poly A tail.
In one embodiment of the present invention, the mRNA is an autonomously amplifying mRNA, i.e. the mRNA comprises, in addition to the mRNA sequence encoding the S protein of the novel coronavirus SARS-CoV-2 or a variant thereof, a 5 ' cap structure, a 5 ' conserved sequence element, an RNA replicase coding region, a subgenomic promoter, a3 ' conserved sequence element and a poly-a tail, wherein the RNA replicase coding region that can be used includes, but is not limited to, alphaviruses (e.g. SFV), picornaviruses (e.g. FMDV), flaviviruses (e.g. DENV), paramyxoviruses (e.g. HMPV) or caliciviruses (e.g. NV), preferably the RNA replicase is encoded by a single conventional mRNA.
In a specific embodiment of the present invention, the mRNA is a trans-amplification mRNA, i.e. the mRNA encoding the target gene comprises a 5 ' cap structure, a 5 ' conserved sequence element, a subgenomic promoter, a3 ' conserved sequence element and a poly-a tail in addition to the mRNA sequence encoding the S protein of the novel coronavirus SARS-CoV-2 or a variant thereof.
Preferably, the mRNA is a monocistronic, bicistronic, or polycistronic mRNA, i.e., an mRNA containing two or more coding regions.
Preferably, the coding regions of the bicistronic or polycistronic mRNA are separated by at least one Internal Ribosome Entry Site (IRES) sequence, which can be used including, but not limited to: small RNA viruses (e.g., FMDV), pestiviruses (e.g., CFFV), poliovirus (e.g., PV), encephalomyocarditis virus (e.g., ECMV), foot and mouth disease virus (e.g., FMDV), hepatitis c virus (e.g., HCV), classical swine fever virus (e.g., CSFV), mouse leukoplakia virus (e.g., MLV), simian immunodeficiency virus (e.g., SIV), or cricket paralysis virus (e.g., CrPV).
Preferably, the length of the mRNA sequence is 1000-20000 nucleotides, and more preferably, the length of the mRNA sequence is 3000-15000 nucleotides.
In a second aspect of the invention, there is provided a protein comprising a variant or derivative of SARS-2-CoV-type coronavirus S protein or a fragment thereof.
Preferably, said variant of S protein comprises SEQ ID NO:26, and further preferably, said variant of S protein fragment comprises SEQ ID NO:27, wherein said SEQ ID NO:26 or SEQ ID NO:27 comprises any one or a combination of two or more of the following:
A) mutation at L611, preferably, L611 to a611 or G611;
B) mutation at position Y612, preferably mutation Y612 to a612 or G612;
C) a mutation at position Q613, preferably Q613 to a613 or G613;
D) a mutation at position D614, preferably, the mutation of D614 is a614 or G614;
E) mutation at position T859, preferably, the T859 mutation is a859 or G859.
Preferably, said mutation of SEQ ID NO 26 or 27 further comprises the mutation at position 682RRAR685 and/or the mutation at position 986KV987, so that the S protein is fixed in the prefusion conformation; further preferred, 682RRAR685 and/or 986KV987 are mutated to GSAG and/or PP.
In a specific embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of L611A in a) is shown as SEQ ID NO:1 or a sequence identical to SEQ ID NO:1, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical; the amino acid sequence of the full-length S protein with the specific mutation site of L611A fixed in the pre-fusion conformation in the A) is shown as SEQ ID NO:2 or a variant of SEQ ID NO:2, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical; the amino acid sequence of the extracellular region of the wild type S protein of which the specific mutation site in A) is L611A is shown as SEQ ID NO: 13 or a sequence identical to SEQ ID NO: 13 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein with the specific mutation site of L611A fixed in the pre-fusion conformation in the A) is shown as SEQ ID NO: 14 or a sequence identical to SEQ ID NO: 14, 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
In a specific embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of Y612A in B) is shown in SEQ ID NO: 3 or a sequence identical to SEQ ID NO: 3 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the full-length S protein which has the specific mutation site of Y612A and is fixed in the pre-fusion conformation in the B) is shown as SEQ ID NO: 4 or a sequence identical to SEQ ID NO: 4 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the wild type S protein of which the specific mutation site in B) is Y612A is shown as SEQ ID NO: 15 or a variant of SEQ ID NO: 15, amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein fixed in the pre-fusion conformation, of which the specific mutation site in B) is Y612A, is shown as SEQ ID NO: 16 or a sequence identical to SEQ ID NO: 16, 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
In a specific embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of Q613A in C) is shown in SEQ ID NO: 5 or a sequence identical to SEQ ID NO: 5 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the full-length S protein with the specific mutation site of Q613A fixed in the pre-fusion conformation in C) is shown as SEQ ID NO: 6 or a sequence identical to SEQ ID NO: 6 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the wild type S protein of which the specific mutation site in C) is Q613A is shown as SEQ ID NO: 17 or a sequence identical to SEQ ID NO: 17 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein fixed in the pre-fusion conformation, of which the specific mutation site in C) is Q613A, is shown as SEQ ID NO: 18 or a variant of SEQ ID NO: 18, 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
In a specific embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of D614G in D) is shown in SEQ ID NO: 7 or a sequence identical to SEQ ID NO: 7 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the full-length S protein which has the specific mutation site of D614G and is fixed in the pre-fusion conformation is shown as SEQ ID NO: 8 or a variant of SEQ ID NO: 8, amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the wild type S protein with the specific mutation site of D614G in D) is shown as SEQ ID NO: 19 or a variant of SEQ ID NO: 19, amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein fixed in the pre-fusion conformation, the specific mutation site of which is D614G in D) is shown as SEQ ID NO: 20 or a variant of SEQ ID NO: 20, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical.
In one embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of 611LYQD614 and 611AAAG614 in the combination of a) -D) is shown as SEQ ID No. 9 or the amino acid sequence of SEQ ID NO:9, amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the full-length S protein fixed in the pre-fusion conformation, of which the specific mutation site in the A) -D) combination is 611LYQD614 and 611AAAG614 is shown as SEQ ID NO: 10 or a variant of SEQ ID NO: 10 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the wild type S protein with the specific mutation site of 611LYQD614 mutated into 611AAAG614 in the combination of A) to D) is shown as SEQ ID NO 21 or the amino acid sequence of the extracellular region of the wild type S protein is similar to SEQ ID NO:21 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein fixed in the pre-fusion conformation of the combination of A) -D) in which the specific mutation site is 611LYQD614 is mutated to 611AAAG614 is shown as SEQ ID NO:22 or a sequence identical to SEQ ID NO:22, 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
In a specific embodiment of the invention, the amino acid sequence of the wild-type full-length S protein with the specific mutation site of T859A in the E) is shown as SEQ ID NO. 11 or an amino acid sequence with 70%, 75%, 80%, 85%, 90%, 95% or 99% of identity with the SEQ ID NO. 11; the amino acid sequence of the full-length S protein which has the specific mutation site of T859A and is fixed in the pre-fusion conformation is shown as SEQ ID NO 12 or the amino acid sequence of the full-length S protein is similar to the amino acid sequence shown as SEQ ID NO:12 amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the wild type S protein of which the specific mutation site is T859A in the E) is shown as SEQ ID NO: 23 or a variant of SEQ ID NO: 23, amino acid sequences having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the amino acid sequence of the extracellular region of the S protein fixed in the pre-fusion conformation, of which the specific mutation site in E) is T859A, is shown as SEQ ID NO: 24 or a variant of SEQ ID NO: 24, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical.
Preferably, the protein further comprises a signal peptide, wherein the signal peptide is a signal peptide which codes a human cell recognizable secretion signal peptide and is selected from a signal peptide of SARS-2-CoV type coronavirus S protein, a signal peptide of tissue plasminogen activator (tPA) and/or a signal peptide of serum immunoglobulin E (lgE), and is further preferably a signal peptide of SARS-2-CoV type coronavirus S protein.
Preferably, the protein further comprises a trimer-forming domain of a helper protein; further preferably, the domain is the Fibritin foldon of the T4 bacteriophage Fibritin protein, and the amino acid sequence is shown in SEQ ID NO: 25 or a sequence identical to SEQ ID NO: 25, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical.
Preferably, said protein further comprises a linker sequence between the sequence of said variant or derivative of the SARS-2-CoV-type coronavirus S protein or fragment thereof and said trimerization domain; more preferably, the ligation sequence is AG.
In a third aspect of the invention, there is provided a composition comprising an mRNA as described above.
Preferably, the composition further comprises a cationic or polycationic compound, wherein the cationic or polycationic compound is free or bound to mRNA, such that the composition is more stable.
Preferably, the composition further comprises liposomes.
Preferably, the liposomes include, but are not limited to, liposomes capable of promoting self-assembly into virus-sized particles (-100 nm), liposomes that allow for intracellular release of mRNA from the endosome, liposomes that support a phospholipid bilayer structure, or liposomes that act as stabilizers.
More preferably, to increase the half-life of LNP, the liposomes can further comprise pegylated liposomes.
In one embodiment of the invention, the liposomes comprise cationic liposomes, pegylated liposomes, cholesterol and/or phospholipids.
Preferably, the composition further comprises a pharmaceutically acceptable excipient selected from carriers, diluents, adjuvants or adjuvant-encoding nucleotide sequences, solubilizers, binders, lubricants, suspending agents or transfection-promoting agents including, but not limited to, surfactants such as immunostimulatory complexes, Freunds incomplete adjuvant, LPS analog (e.g., monophosphoryl ester a), muramyl peptide, benzoquinone analog, squalene, hyaluronic acid, lipids, liposomes, calcium ions, viral proteins, cations, polycations (e.g., poly-L-glutamic acid (LGS)) or nanoparticles or other known transfection-promoting agents, such adjuvant-encoding nucleotide sequences being nucleotide sequences encoding at least one of the following adjuvants: GM-CSF, IL-17, IFNg, IL-15, IL-21, anti-PD 1/2, lactoferrin, protamine, IL-1, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, INF- α, INF- γ, Lymphotoxin- α, hGH, MCP-1, MIP-1a, MIP-1P, IL-8, RANTES, L-selectin, P-selectin, E-selectin, CD34, GlyCAM-1, MadCAM-1, LFA-1, VLA-1, Mac-1, pl50.95, PECAM, ICAM-1, ICAM-2, ICAM-3, CD2, LFA-3, M-CSF, CD40, CD40L, CD40, CD L, Vascular growth factor, fibroblast growth factor, nerve growth factor, vascular endothelial growth factor, Apo-1, p55, WSL-1, DR3, TRAMP, Apo-3, AIR, LARD, NGRF, DR4, DR5, KILLER, TRAIL-R2, TRICK2, DR6, caspase ICE, Fos, c-jun, Sp-1, Ap-2, p38, p65Rel, MyD88, IRAK, TRAF6, IkB, inactive NIK, SAP K, SAP-1, JNK, NFkB, Bax, TAP, TRAILrec, TRAILrecDRC5, TRAIL-R3, TRAIL-R4, RANK, RANK LIGAND, 40, Ox40LIGAND D, NKG2, NKA, MICB, NKG2, NKG A, NKG2, NKG B, NKG2, NKG 867, NKG 8672, NKG 8646, NKG 9372, NKG 8646, NKG2, and functional fragments thereof.
Preferably, the composition is liposome, lipid complex or lipid nanoparticle, and the liposome can be prepared in the following forms: 1, 2-dioleyloxy-N, N-dimethylaminopropane (DODMA) liposomes, 1, 2-dioleyloxy-3-dimethylaminopropane (DLin-DMA), 2-dioleyl-4- (2-dimethylaminoethyl) - [1, 3] -dioxolane (DLin-KC2-DMA) liposomes. The lipid complex or lipid nanoparticle may be formed from a lipid selected from the group consisting of: DLin-DMA, DLin-K-DMA, 98N12-5, C12-200, DLin-MC3-DMA, DLin-KC2-DMA, DODMA, PLGA, PEG-DMG, pegylated lipids, and aminoalcohol lipids.
In a fourth aspect of the invention, there is provided a composition comprising a protein as described above.
Preferably, the composition further comprises a pharmaceutically acceptable excipient selected from a carrier, diluent, adjuvant or nucleotide sequence encoding an adjuvant, solubilizer, binder, lubricant, suspending agent or transfection facilitating agent including, but not limited to, a surfactant such as immunostimulatory complexes, Freunds incomplete adjuvant, LPS analog (e.g., monophosphoryl ester a), muramyl peptide, benzoquinone analog, squalene, hyaluronic acid, lipid, liposome, calcium ion, viral protein, cation, polycation (e.g., poly-L-glutamic acid (LGS)), or nanoparticle or other known transfection facilitating agent.
In a fifth aspect of the invention, there is provided a nucleic acid encoding the protein described above.
Preferably, the nucleic acid may be mRNA or DNA.
Preferably, the nucleic acid is a nucleotide sequence of an epitope antigen gene of the protein.
In a sixth aspect of the invention, there is provided a vector comprising the nucleic acid described above.
In a seventh aspect of the present invention, there is provided a cell comprising the above-mentioned mRNA, the above-mentioned protein, the above-mentioned nucleic acid and/or the above-mentioned vector.
In an eighth aspect of the present invention, there is provided a vaccine comprising the mRNA, the protein, the composition, the nucleic acid, the vector, or the cell.
In a ninth aspect of the present invention, there is provided a method for preparing a composition or vaccine comprising mRNA, said method comprising mixing said mRNA with a cationic or polycationic compound and then encapsulating the mixture in liposomes.
Preferably, the liposomes include, but are not limited to, liposomes that promote self-assembly into virus-sized particles (-100 nm), liposomes that allow for intracellular release of mRNA from endosomes, liposomes that support a phospholipid bilayer structure, or liposomes that act as stabilizers, and more preferably, to increase the half-life of LNP, the liposomes can further comprise pegylated liposomes.
In one embodiment of the invention, the liposomes comprise cationic liposomes, pegylated liposomes, cholesterol and/or phospholipids.
In a tenth aspect of the invention, there is provided a process for the preparation of a composition or vaccine comprising a protein, said process comprising admixing a protein as defined above with an excipient.
Preferably, the pharmaceutically acceptable excipient is selected from a carrier, diluent, adjuvant or nucleotide sequence encoding an adjuvant, solubilizer, binder, lubricant, suspending agent or transfection facilitating agent including, but not limited to, a surfactant such as an immunostimulatory complex, Freunds incomplete adjuvant, LPS analog (e.g., monophosphoryl ester a), muramyl peptide, benzoquinone analog, squalene, hyaluronic acid, lipid, liposome, calcium ion, viral protein, cation, polycation (e.g., poly-L-glutamic acid (LGS)) or nanoparticle or other known transfection facilitating agent.
In the eleventh aspect of the present invention, there is provided the use of the above-mentioned mRNA, the above-mentioned protein, the above-mentioned composition, the above-mentioned nucleic acid, the above-mentioned vector or the above-mentioned cell for the prevention and/or treatment of SARS-CoV-2 coronavirus infection.
In the twelfth aspect of the present invention, there is provided the use of the above-mentioned mRNA, the above-mentioned protein, the above-mentioned composition, the above-mentioned nucleic acid, the above-mentioned vector or the above-mentioned cell for the preparation of a medicament for preventing and/or treating SARS-CoV-2 coronavirus infection.
Preferably, said prevention comprises using the mRNA or mRNA-containing composition or the protein or protein-containing composition of the invention as a vaccine.
Preferably, the treatment comprises screening for antibodies that bind to the mRNA or mRNA-containing composition or protein-containing composition of the invention and using them in the treatment.
In a thirteenth aspect of the invention, there is provided a method for preventing infection by SARS-CoV-2 type coronavirus, comprising administering to an individual not infected with SARS-CoV-2 type coronavirus an effective amount of an mRNA or a composition comprising an mRNA or a protein or a composition comprising a protein according to the invention.
In a fourteenth aspect of the invention, there is provided a method of treating a SARS-CoV-2 type coronavirus infection, comprising administering to an individual infected with a SARS-CoV-2 type coronavirus an effective amount of an mRNA or a composition comprising an mRNA or a protein or a composition comprising a protein according to the invention. So that the individual generates neutralizing antibodies against the SARS-CoV-2 coronavirus in vivo.
In a fifteenth aspect of the present invention, there is provided a method of antibody screening comprising the step of administering to an individual an effective amount of the mRNA or mRNA-containing composition or protein-containing composition of the present invention.
Wherein the antibody screening method is not a therapeutic method. The method is used for screening neutralizing antibodies, detecting and comparing the drug effects of the antibodies to determine which antibodies can be used as drugs and which can not be used as drugs, or comparing the drug effect sensitivity degrees of different drugs, namely, the treatment effect is not necessary and is only a possibility.
In a sixteenth aspect of the invention there is provided a method of inducing a neutralizing antigen-specific immune response in an individual comprising administering to the individual a composition comprising mRNA or a protein or a composition comprising a protein according to the invention.
Preferably, the antigen-specific immune response comprises a T cell response and/or a B cell response.
The mRNA and the composition containing the mRNA have the following advantages: 1. in vitro synthesis, cell culture is not needed, and the risk of animal source pollution is avoided; 2. the development and production are fast, the standardized production is realized, the mass production and the quality control are easy, and the same production flow is suitable for a plurality of different products; 3. can be expressed continuously in a period of time, and the antigen exposure time is prolonged, so that the strength and quality of immune response are improved; 4. simulating the natural infection process, being translated and modified in human cells, and being presented by MHC class I molecules to induce stronger cellular immunity; 5. supports various protein forms including intracellular protein, transmembrane protein, VLP and the like, and can avoid the purification problem caused by low yield of VLP; 6. the self-adjuvant effect is realized, and adjuvant screening is not needed; 7. no risk of infection and genome integration; 8. the vaccine has no pre-existing immunity and can be used for multiple times of immunity.
The S protein of the present invention is the structural protein of SARS-CoV-2 type coronavirus and named spike protein.
The 'LNP' of the invention is a lipid nanoparticle.
"identity" as used herein means that, in the context of using an amino acid sequence or a nucleotide sequence, one skilled in the art can adjust the sequence as needed to have (including but not limited to) 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 70%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9% similarity, and still have the same function as the original amino acid sequence or nucleotide sequence.
The "individual" according to the present invention includes mammals and humans. Such mammals include, but are not limited to, rodents (e.g., mice, rats), monkeys, zebrafish, pigs, chickens, rabbits, and the like.
"prevention" as referred to herein means all actions to avoid symptoms or to delay the stress of a particular symptom by administering a product as described herein before or after the onset of disease development.
"treatment" as used herein refers to a therapeutic intervention that ameliorates the signs, symptoms, etc. of a disease or pathological state after the disease has begun to develop.
An "effective amount" as referred to herein, refers to an amount or dose of a product of the invention which provides the desired treatment or prevention after administration to a patient or organ in single or multiple doses.
Drawings
Embodiments of the invention are described in detail below with reference to the attached drawing figures, wherein:
FIG. 1: sequence map of a conventional mRNA plasmid containing the T7 promoter, 5 'UTR, 3' UTR, and polyA tail.
FIG. 2: schematic representation of a self-replicating mRNA plasmid template containing a T7 promoter, 5 'conserved sequence elements, an RNA replicase coding region, a subgenomic promoter, 3' conserved sequence elements, and a poly a tail.
FIG. 3: and (2) detecting results of formaldehyde denatured gel of uncapped mRNA and capped mRNA, wherein M is marker, 1-6 are uncapped mRNA of the full-length S protein which is fixed in the pre-fusion conformation and codes a specific mutation site, the mutation sites of 1-6 are L611A, Y612A, Q613A, D614G and 611LYQD614 are mutated into 611AAAG614 and T859A respectively, 7-12 are capped mRNA of the full-length S protein which is fixed in the pre-fusion conformation and codes the specific mutation site, and the mutation sites of 7-12 are L611A, Y612A, Q613A, D614G and 611LYQD614 are mutated into 611AAAG614 and T859A respectively.
FIG. 4: and (3) performing in-vitro expression immunofluorescence detection on the mutation construction.
FIG. 5: western Blot detection results of HEK293 cells and culture supernatants.
FIG. 6: and (3) constructing the contents of tripolymers and monomers in the expressed protein by the purified mutation, wherein A is a high performance liquid chromatogram, and B is the peak area result of the tripolymers and the monomers in the high performance liquid chromatogram.
FIG. 7: and (3) detecting the affinity of each mutation construction expression protein and an ACE2 dimer, wherein A is a BLI detection result graph, and B is the Koff, Kon and KD value of each mutation construction expression protein in the BLI detection result graph, wherein the Koff is an association rate constant, the Kon is an association rate constant, and the KD is an equilibrium dissociation constant.
FIG. 8: and (5) detecting the titer of the mRNA vaccine neutralizing antibody.
FIG. 9: and (5) detecting the titer of the specific antibody of the mRNA vaccine.
FIG. 10: and (5) detecting the titer of the neutralizing antibody of the subunit vaccine.
FIG. 11: and (5) detecting the titer of the subunit vaccine specific antibody.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1: preparation and immunization of traditional mRNA vaccines
1. mRNA preparation and detection
1) A conventional mRNA plasmid template was synthesized, the sequence of which is shown in FIG. 1, and linearized with the restriction endonuclease BsmBI.
2) Artificially synthesizing a coded amino acid sequence SEQ ID NO: 2. 4, 6, 8, 10, 12, or a nucleotide sequence of SEQ ID NO: 28-33, and respectively linked into linearized traditional mRNA plasmid templates by means of homologous recombination.
3) And respectively transforming the plasmids subjected to homologous recombination into Xl1-Blue strains, and sequencing to confirm that the sequences are correct and the transcription template is successfully constructed. And (3) fermenting the strain by using a shake flask, and purifying by using an endotoxin-free plasmid macroextraction kit to obtain a transcription template.
4) The transcription template was linearized with the restriction endonuclease BbsI. Transcription was performed using the T7 in vitro transcription kit to obtain uncapped mRNA. The transcription templates were digested with DNaseI, respectively, and the mRNA was purified by lithium chloride precipitation. The mRNA was capped with Cap1 capping kit and purified by lithium chloride precipitation to obtain capped mRNA.
5) The uncapped and capped mrnas were detected using formaldehyde-denatured glue, as shown in fig. 3, in which 1-6 were uncapped mrnas encoding full-length S protein immobilized in the pre-fusion conformation at the specific mutation site, L611A, Y612A, Q613A, D614G, 611LYQD614 were mutated to 611AAAG614, T859A, 7-12 were capped mrnas encoding full-length S protein immobilized in the pre-fusion conformation at the specific mutation site, and L611A, Y612A, Q613A, D614G, 611LYQD614 were mutated to 611AAAG614, T859A, respectively, at the mutation sites of 7-12. The correct size of the mRNA was seen with essentially no degradation.
6) Immunofluorescence was used to analyze the expression profile of all mutant constructs in vitro. HEK293 cells in a 24-well plate were transfected with 0.5. mu.g of capped mRNA per well using lipofectamine 2000 as a transfection reagent, and were detected with anti-S protein polyclonal antibody 24 hours after transfection, and the detection results are shown in FIG. 4. The expression levels of the mutant constructs were reduced to different extents, but all constructs were expressed.
2. mRNA packaging
1) Dissolving and mixing four components of cationic liposome D-Lin-MC3-DMA, distearoyl phosphatidylcholine DSPC, cholesterol and PEG liposome PEG-DMG in ethanol according to a molar ratio of 50:10:38.5: 1.5.
2) The preparation method comprises the following steps of: the mRNA flow rate ratio was 1:3, and mixed packaging was performed in Precision Nanosystems' nanoparticle preparation apparatus Ignite. The packaged mRNA-LNP is dialyzed and concentrated by ultrafiltration into DPBS, and samples for animal experiments are obtained after sterile filtration.
3. Immunization of mice
BALB/c mice (n is 6/group) are immunized with the mRNA-LNP vaccine on the 0 th day and the 14 th day together, the immunization dose is 5ug of mRNA-LNP/dose, and blood collection on the 28 th day is used for detecting S protein specific antibody titer and neutralizing antibody titer. Although there was no significant difference in S protein specific antibody titers by the mutant and non-mutant immunizations (as shown in fig. 9), the neutralizing antibody titer was significantly higher for each mutant (as shown in fig. 8), with the highest neutralizing antibody titer for the T859A mutant mRNA vaccine. In conclusion, the conventional mRNA-LNP vaccine prepared in this example can be used for prevention of SARS-CoV-2 type coronavirus infection, and the induced neutralizing antibody titer is higher than that of the non-mutant type.
Example 2 preparation and immunization of subunit vaccines
1. Protein expression and detection
1) Artificially synthesizing a coded amino acid sequence SEQ ID NO: 14. 16, 18, 20, 22, 24 and the trimeric domain and is ligated into the pcdna3.4 vector.
2) And respectively transforming the plasmids subjected to homologous recombination into DH5a strains, and sequencing to confirm that the sequences are correct and the transcription template is successfully constructed. The strain is fermented by a shake flask, and purified by a large endotoxin-free plasmid extraction kit to obtain the plasmid.
3) The plasmid was transiently transfected into HEK293 suspension cells by PEI, and cultured for another 6 days, and the cells were removed by centrifugation to obtain a culture supernatant.
4) On days 3-6 after transfection, cells were separated from the culture supernatant by sampling each day and examined with WB as shown in FIG. 5. It can be seen that only three mutations D614G, Q613A and T859A are constructed with higher expression level, and the protein can be correctly folded and secreted to the extracellular.
2. Protein purification and detection
And purifying the culture supernatant by using a Ni column to obtain the purified protein. The purified protein was tested for trimer and monomer content by HPLC, as shown in FIG. 6, it can be seen that three mutant constructs can completely fix the protein in the trimer structure. The affinity of each sample and the ACE2 dimer was examined with BLI and, as shown in fig. 7, the affinity of the three mutant constructs for the ACE2 dimer was 10-fold higher than that of the non-mutant prefusion conformation or the extracellular domain of the wild-type S protein, with the highest affinity being T859A.
3. Immunization of mice
And mixing the protein solution with an equivalent amount of aluminum hydroxide adjuvant to prepare the subunit vaccine. BALB/c mice (n 6/group) were immunized at 5ug protein/dose, two-needle subunit vaccines were co-immunized on day 0 and 14, and blood was collected on day 28 for S protein-specific and neutralizing antibody titers. Although there was no significant difference in S protein specific antibody titers by the mutant and non-mutant immunizations (as shown in fig. 11), the neutralizing antibody titer by each mutant was significantly higher (as shown in fig. 10), with the T859A mutant subunit vaccine giving the highest neutralizing antibody titer, consistent with the mRNA vaccine immunization results. In conclusion, the subunit vaccine prepared in this example can be used for prevention of SARS-CoV-2 coronavirus infection, and the induced neutralizing antibody titer is higher than that of the non-mutant type.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.
Sequence listing
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<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 2
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Ala Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 3
<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 3
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Ala Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 4
<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 4
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Ala Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 5
<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 5
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Ala Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 6
<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 6
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Ala Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 7
<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 7
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 8
<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 8
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 9
<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 9
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Ala Ala Ala Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 10
<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 10
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Ala Ala Ala Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 11
<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 11
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Ala Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 12
<211> 1273
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 12
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Ala Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 13
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 13
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Ala Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 14
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 14
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Ala Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 15
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 15
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Ala Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 16
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 16
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Ala Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 17
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 17
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Ala Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 18
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 18
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Ala Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 19
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 19
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 20
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 20
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 21
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 21
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Ala Ala Ala Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 22
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 22
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Ala Ala Ala Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 23
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 23
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Ala Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 24
<211> 1208
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 24
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Pro Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Gly Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Ala Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 25
<211> 27
<212> PRT
<213> T4 Phage
<400> 25
Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp
1 5 10 15
Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Gly
20 25
<210> 26
<211> 1273
<212> PRT
<213> SARS-2-CoV
<400> 26
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
1205 1210 1215
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
1220 1225 1230
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
1235 1240 1245
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val
1250 1255 1260
Leu Lys Gly Val Lys Leu His Tyr Thr
1265 1270
<210> 27
<211> 1208
<212> PRT
<213> SARS-2-CoV
<400> 27
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
1 5 10 15
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
20 25 30
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
35 40 45
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
50 55 60
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
65 70 75 80
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
85 90 95
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
100 105 110
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
115 120 125
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
130 135 140
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145 150 155 160
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
165 170 175
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
180 185 190
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
195 200 205
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
210 215 220
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225 230 235 240
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
245 250 255
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
260 265 270
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
275 280 285
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
290 295 300
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305 310 315 320
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
325 330 335
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
340 345 350
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
355 360 365
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
370 375 380
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385 390 395 400
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
405 410 415
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
420 425 430
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
435 440 445
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
450 455 460
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465 470 475 480
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
485 490 495
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
500 505 510
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
515 520 525
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
530 535 540
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545 550 555 560
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
565 570 575
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
580 585 590
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
595 600 605
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile
610 615 620
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625 630 635 640
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val
645 650 655
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
660 665 670
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
675 680 685
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
690 695 700
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705 710 715 720
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
725 730 735
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
740 745 750
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
755 760 765
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
770 775 780
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785 790 795 800
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
805 810 815
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
820 825 830
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
835 840 845
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
850 855 860
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865 870 875 880
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
885 890 895
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
900 905 910
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
915 920 925
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
930 935 940
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945 950 955 960
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
965 970 975
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
980 985 990
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
995 1000 1005
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
1010 1015 1020
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025 1030 1035 1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
1045 1050 1055
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
1060 1065 1070
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
1075 1080 1085
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
1090 1095 1100
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105 1110 1115 1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
1125 1130 1135
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
1140 1145 1150
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
1155 1160 1165
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp
1170 1175 1180
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185 1190 1195 1200
Gln Glu Leu Gly Lys Tyr Glu Gln
1205
<210> 28
<211> 3822
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 28
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg gcctatcagg acgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gcaagttcga cgaggacgac 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct ag 3822
<210> 29
<211> 3822
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 29
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg ctggcccagg acgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gcaagttcga cgaggacgac 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct ag 3822
<210> 30
<211> 3822
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 30
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg ctgtatgccg acgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gcaagttcga cgaggacgac 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct ag 3822
<210> 31
<211> 3822
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 31
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg ctgtatcagg gcgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gcaagttcga cgaggacgac 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct ag 3822
<210> 32
<211> 3822
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 32
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg gccgccgccg gcgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gcaagttcga cgaggacgac 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct ag 3822
<210> 33
<211> 3822
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 33
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg ctgtatcagg acgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctggccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gcaagttcga cgaggacgac 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct ag 3822
<210> 34
<211> 3828
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 34
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg gcctatcagg acgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gtaagtttga tgaggacgat 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct gataatag 3828
<210> 35
<211> 3828
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 35
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg ctggcccagg acgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gtaagtttga tgaggacgat 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct gataatag 3828
<210> 36
<211> 3828
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 36
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg ctgtatgccg acgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gtaagtttga tgaggacgat 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct gataatag 3828
<210> 37
<211> 3828
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 37
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg ctgtatcagg gcgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gtaagtttga tgaggacgat 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct gataatag 3828
<210> 38
<211> 3828
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 38
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg gccgccgccg gcgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gtaagtttga tgaggacgat 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct gataatag 3828
<210> 39
<211> 3828
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 39
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg ctgtatcagg acgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctggccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcagtacatc aagtggccct ggtatatctg gctgggcttc 3660
atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacaagctgc 3720
tgttcctgcc tgaagggctg ctgttcttgt ggcagctgct gtaagtttga tgaggacgat 3780
agcgagcctg tgctgaaggg cgtgaagctg cactacacct gataatag 3828
<210> 40
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 40
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg gcctatcagg acgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 41
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 41
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg ctggcccagg acgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 42
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 42
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg ctgtatgccg acgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 43
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 43
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg ctgtatcagg gcgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 44
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 44
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg gccgccgccg gcgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 45
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 45
atgttcgtgt ttctggtgct gctgcctctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccccctgc ctataccaat tccttcacac ggggcgtgta ctatcccgac 120
aaggtgttta gatctagcgt gctgcactcc acacaggatc tgtttctgcc tttcttttct 180
aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac 240
aatccagtgc tgccctttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc 300
atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg 360
aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatccattc 420
ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tcgcgtgtat 480
tcctctgcca acaattgcac atttgagtac gtgtcccagc ccttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgagg gagttcgtgt ttaagaatat cgatggctac 600
ttcaagatct actccaagca caccccaatc aacctggtgc gcgacctgcc acagggcttc 660
tctgccctgg agccactggt ggatctgccc atcggcatca acatcacccg gtttcagaca 720
ctgctggccc tgcacagaag ctacctgaca ccaggcgaca gctcctctgg atggaccgca 780
ggagcagcag cctactatgt gggctatctg cagcccagga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgccgtggat tgcgccctgg atcccctgtc tgagaccaag 900
tgtacactga agagctttac cgtggagaag ggcatctatc agacaagcaa tttcagggtg 960
cagcctaccg agtccatcgt gcgctttccc aatatcacaa acctgtgccc ttttggcgag 1020
gtgttcaacg caaccaggtt cgcaagcgtg tacgcatgga ataggaagcg catctccaac 1080
tgcgtggccg actattctgt gctgtacaac agcgcctcct tctctacctt taagtgctat 1140
ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcaggg gcgacgaggt gcgccagatc gcaccaggac agacaggcaa gatcgcagac 1260
tacaattata agctgcctga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat 1320
ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtctaat 1380
ctgaagccat tcgagaggga catctccaca gagatctacc aggccggctc taccccctgc 1440
aatggcgtgg agggctttaa ctgttatttc cctctgcaga gctacggctt ccagccaaca 1500
aacggcgtgg gctatcagcc ctaccgcgtg gtggtgctgt cttttgagct gctgcacgca 1560
cctgcaacag tgtgcggacc aaagaagagc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccttttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgaccctcag 1740
accctggaga tcctggacat cacaccatgc tccttcggcg gcgtgtctgt gatcacacca 1800
ggcaccaata caagcaacca ggtggccgtg ctgtatcagg acgtgaattg taccgaggtg 1860
ccagtggcaa tccacgcaga tcagctgacc cctacatggc gggtgtactc taccggcagc 1920
aacgtgttcc agacaagagc aggatgcctg atcggagcag agcacgtgaa caatagctat 1980
gagtgcgaca tccctatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc 2040
ccaaggagag cacggtctgt ggcaagccag tccatcatcg cctataccat gagcctgggc 2100
gccgagaatt ccgtggccta ctccaacaat tctatcgcca tccctaccaa cttcacaatc 2160
tccgtgacca cagagatcct gccagtgagc atgaccaaga catccgtgga ctgcacaatg 2220
tatatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtacgg ctctttttgt 2280
acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aacttcagcc agatcctgcc cgatcctagc aagccatcca agcggtcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgccgccag agacctgatc tgtgcccaga agtttaatgg cctggccgtg 2580
ctgcctccac tgctgacaga tgagatgatc gcccagtaca catctgccct gctggcagga 2640
accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg 2700
cagatggcct atcggttcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgagctcc 2820
acagcaagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat 2880
accctggtga agcagctgtc tagcaacttc ggcgccatct cctctgtgct gaatgacatc 2940
ctgagcaggc tggacaaggt ggaggcagag gtgcagatcg accggctgat cacaggcaga 3000
ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggca 3060
tctgccaatc tggccgccac caagatgagc gagtgcgtgc tgggccagtc caagagagtg 3120
gacttttgtg gcaagggcta tcacctgatg agcttcccac agtccgcccc tcacggagtg 3180
gtgtttctgc acgtgaccta cgtgccagcc caggagaaga acttcaccac agcaccagca 3240
atctgccacg atggcaaggc acactttcct agggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagcg caatttctac gagccacaga tcatcaccac agacaataca 3360
ttcgtgtccg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtatgatcct 3420
ctgcagccag agctggactc ttttaaggag gagctggata agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctct ggcatcaatg ccagcgtggt gaacatccag 3540
aaggagatcg acaggctgaa cgaggtggcc aagaatctga acgagtccct gatcgatctg 3600
caggagctgg gcaagtatga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 46
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 46
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg gcctatcagg acgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 47
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 47
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg ctggcccagg acgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 48
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 48
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg ctgtatgccg acgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 49
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 49
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg ctgtatcagg gcgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 50
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 50
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg gccgccgccg gcgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctgaccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720
<210> 51
<211> 3720
<212> DNA/RNA
<213> Artificial Sequence (Artificial Sequence)
<400> 51
atgttcgtgt ttctggtgct gctgcccctg gtgagctccc agtgcgtgaa cctgaccaca 60
aggacccagc tgccacctgc ctataccaac agcttcacaa gaggcgtgta ctatccggac 120
aaggtgtttc gctccagcgt gctgcactct acacaggatc tgtttctgcc attcttcagc 180
aacgtgacct ggttccacgc catccacgtg tccggcacca atggcacaaa gagattcgac 240
aatccagtgc tgcccttcaa cgatggcgtg tacttcgcga gcaccgagaa gtccaacatc 300
atccgcggct ggatctttgg caccacactg gactccaaga cacagagcct gctgatcgtg 360
aacaacgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgcaa cgatcccttc 420
ctgggcgtgt actatcacaa gaacaacaag tcctggatgg aguccgagtt tcgcgtgtat 480
tcctctgcca acaattgcac attcgagtac gtgtcccagc ctttcctgat ggacctggag 540
ggcaagcagg gcaatttcaa gaacctgcgg gagttcgtgt tcaagaatat cgatggctac 600
ttcaagatct actccaagca cacccctatc aacctggtga gggacctgcc acagggcttc 660
tctgccctgg agcctctggt ggatctgcca atcggcatca acatcaccag atttcagaca 720
ctgctggctc tgcaccgcag ctacctgaca cccggcgaca gctcctctgg atggaccgct 780
ggagctgctg cttactatgt gggctatctg cagcctcgga ccttcctgct gaagtacaac 840
gagaatggca ccatcacaga cgctgtggat tgcgccctgg atcctctgag cgagaccaag 900
tgtacactga agtcctttac cgtggagaag ggcatctatc agacatccaa tttccgggtg 960
cagccaaccg agtctatcgt gaggtttcca aatatcacaa acctgtgccc ctttggcgag 1020
gtgttcaacg ctaccaggtt cgccagcgtg tacgcttgga acagaaagcg catctccaac 1080
tgcgtggcgg actacagcgt gctgtacaac agcgcctcct tctctacctt caagtgctat 1140
ggcgtgagcc ctacaaagct gaacgacctg tgctttacca acgtgtacgc cgattccttc 1200
gtgatcagag gcgacgaggt gcgccagatc gctccaggac agacaggcaa gatcgccgac 1260
tacaattaca agctgcccga cgatttcacc ggctgcgtga tcgcctggaa ctccaacaat 1320
ctggattcca aagtgggcgg caactacaat tatctgtacc ggctgttcag gaagtccaat 1380
ctgaagccct tcgagcggga catctctaca gagatctacc aggccggcag caccccttgc 1440
aatggcgtgg agggcttcaa ctgttatttc cccctgcagt cttacggctt ccagcctaca 1500
aacggcgtgg gctatcagcc atacagggtg gtggtgctgt ctttcgagct gctgcacgct 1560
ccagctacag tgtgcggacc caagaagtcc accaatctgg tgaagaacaa gtgcgtgaac 1620
ttcaacttca acggactgac cggaacaggc gtgctgaccg agtccaacaa gaagttcctg 1680
ccatttcagc agttcggccg ggacatcgcc gataccacag acgctgtgag ggacccccag 1740
accctggaga tcctggatat cacaccttgc agcttcggcg gcgtgtccgt gatcacacca 1800
ggaaccaata caagcaacca ggtggccgtg ctgtatcagg acgtgaattg taccgaggtg 1860
ccagtggcta tccacgccga tcagctgacc cccacatgga gagtgtactc taccggcagc 1920
aacgtgtttc agacaagggc tggatgtctg atcggagccg agcacgtgaa caacagctac 1980
gagtgcgaca tcccaatcgg cgccggcatc tgtgcttcct accagaccca gacaaactct 2040
ccaggctccg ccggcagcgt ggccagccag tccatcatcg cttataccat gtccctgggc 2100
gccgagaatt ctgtggctta ctccaacaat tctatcgcca tccccaccaa cttcacaatc 2160
agcgtgacca cagagatcct gcccgtgagc atgaccaaga caagcgtgga ctgcacaatg 2220
tatatctgtg gcgacagcac cgagtgctcc aacctgctgc tgcagtacgg ctccttttgt 2280
acccagctga atcgcgccct gacaggcatc gctgtggagc aggacaagaa cacacaggag 2340
gtgttcgccc aggtgaagca gatctacaag accccaccca tcaaggactt tggcggcttc 2400
aatttttccc agatcctgcc cgatcccagc aagccttcca agagatcttt tatcgaggac 2460
ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta tggcgattgc 2520
ctgggcgaca tcgctgccag ggacctgatc tgtgctcaga agttcaatgg cctggccgtg 2580
ctgcctccac tgctgacaga cgagatgatc gctcagtaca caagcgccct gctggctgga 2640
accatcacat ccggctggac cttcggcgct ggagctgccc tgcagatccc atttgccatg 2700
cagatggctt acagattcaa cggcatcggc gtgacccaga atgtgctgta cgagaaccag 2760
aagctgatcg ccaatcagtt caactccgct atcggcaaga tccaggacag cctgagcagc 2820
acagcttccg ccctgggcaa gctgcaggat gtggtgaatc agaacgctca ggccctgaat 2880
accctggtga agcagctgtc gagcaacttc ggcgctatct cctctgtgct gaacgatatc 2940
ctgagccggc tggaccctcc agaggcggag gtgcagatcg accggctgat cacaggcagg 3000
ctgcagtccc tgcagaccta tgtgacacag cagctgatca gagctgccga gatccgcgct 3060
tctgccaatc tggctgccac caagatgtcc gagtgcgtgc tgggccagtc gaagcgcgtg 3120
gacttttgtg gcaagggcta tcacctgatg tctttcccac agagcgcccc acacggagtg 3180
gtgtttctgc acgtgaccta cgtgcctgcc caggagaaga acttcaccac agctccagcc 3240
atctgccacg atggcaaggc ccactttcca cgggagggcg tgttcgtgag caacggcacc 3300
cactggtttg tgacacagag gaatttctac gagccccaga tcatcaccac agacaatacc 3360
ttcgtgagcg gcaactgcga cgtggtcatc ggcatcgtga acaataccgt gtacgatcct 3420
ctgcagccag agctggactc tttcaaggag gagctggaca agtacttcaa gaatcacacc 3480
agccccgacg tggatctggg cgacatctcc ggcatcaatg cttctgtggt gaacatccag 3540
aaggagatcg accgcctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg 3600
caggagctgg gcaagtacga gcaggctgga tacatcccag aggctcctag ggacggacag 3660
gcttacgtga ggaaggatgg cgagtgggtg ctgctgagca cctttctggg atgataatag 3720

Claims (23)

1. A protein comprising a variant or derivative of a SARS-2-CoV-type coronavirus S protein, or a fragment thereof, said variant of S protein comprising the amino acid sequence set forth in SEQ ID NO:26, and a variant of said S protein fragment comprising SEQ ID NO:27, wherein said SEQ ID NO:26 or SEQ ID NO: the mutation of 27 comprises any one or a combination of two or more of the following:
A) mutation at L611, preferably, L611 to a611 or G611;
B) mutation at position Y612, preferably mutation Y612 to a612 or G612;
C) a mutation at position Q613, preferably Q613 to a613 or G613;
D) a mutation at position D614, preferably, the mutation of D614 is a614 or G614;
E) mutation at position T859, preferably, the T859 mutation is a859 or G859.
2. The protein of claim 1, wherein the amino acid sequence of SEQ ID NO:26 or SEQ ID NO:27 further comprises a mutation at position 682RRAR685 and/or a mutation at position 986KV987 to fix the S protein to the prefusion conformation; preferably, 682RRAR685 is mutated to GSAG and/or 986KV987 is mutated to PP.
3. The protein of claim 1 or 2, wherein the variant of the S protein comprises the amino acid sequence as set forth in SEQ ID NO: 1-12 or a variant of any of SEQ ID NOs: 1-12, having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity; the variant of the S protein fragment comprises the amino acid sequence shown as SEQ ID NO: 13-24 or a variant of any of SEQ ID NOs: 13-24, having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
4. The protein according to any one of claims 1 to 3, wherein said protein further comprises a signal peptide selected from the group consisting of a signal peptide of SARS-2-CoV-type coronavirus S protein, a signal peptide of tissue-type plasminogen activator and/or a signal peptide of serum immunoglobulin E.
5. The protein of any one of claims 1 to 4, further comprising a trimer-forming domain of a helper protein, preferably said domain is the domain of the T4 bacteriophage fibritin protein having the amino acid sequence set forth in SEQ ID NO: 25 or a sequence identical to SEQ ID NO: 25, 70%, 75%, 80%, 85%, 90%, 95%, 99% identical.
6. An mRNA comprising an mRNA sequence encoding a variant of a SARS-2-CoV-type coronavirus S protein or fragment thereof; the variant of the S protein comprises SEQ ID NO:26, and a variant of said S protein fragment comprising SEQ ID NO:27, and said SEQ ID NO:26 or SEQ ID NO:27 comprises any one or a combination of two or more of the following:
A) mutation at L611, preferably, L611 to a611 or G611;
B) mutation at position Y612, preferably mutation Y612 to a612 or G612;
C) a mutation at position Q613, preferably Q613 to a613 or G613;
D) a mutation at position D614, preferably, the mutation of D614 is a614 or G614;
E) mutation at position T859, preferably, the T859 mutation is a859 or G859.
7. The mRNA of claim 6, wherein the mRNA of SEQ ID NO:26 or SEQ ID NO:27 further comprises a mutation at position 682RRAR685 and/or a mutation at position 986KV987 to fix the S protein to the prefusion conformation; preferably, 682RRAR685 is mutated to GSAG and/or 986KV987 is mutated to PP.
8. The mRNA of claim 6 or 7, wherein the variant of the S protein is as set forth in SEQ ID NO: 1-12 or a variant of any of SEQ ID NOs: 1-12, and a variant of said S protein fragment comprising an amino acid sequence as set forth in any one of SEQ ID NOs: 13-24 or a variant of any of SEQ ID NOs: 13-24, having 70%, 75%, 80%, 85%, 90%, 95%, 99% identity.
9. The mRNA of any one of claims 6 to 8, wherein the mRNA has a nucleotide sequence as set forth in any one of SEQ ID Nos. 28 to 51 or a nucleotide sequence having 70%, 75%, 80%, 85%, 90%, 95% or 99% identity to any one of SEQ ID Nos. 28 to 51.
10. The mRNA of any one of claims 6 to 9, further comprising a sequence encoding a signal peptide selected from the group consisting of a signal peptide of SARS-2-CoV-type coronavirus S protein, a signal peptide of tissue-type plasminogen activator, and a signal peptide of serum immunoglobulin E.
11. The mRNA of any one of claims 6 to 10, wherein the mRNA further comprises a 5 ' cap structure, a 5 ' noncoding region, a3 ' noncoding region and/or a poly A tail mRNA sequence; or the mRNA also comprises one or the combination of more than two of 5 ' conserved sequence elements, RNA replicase coding regions, subgenomic promoters, 3 ' conserved sequence elements or 3 ' non-coding regions; alternatively, the mRNA further comprises a 5 ' cap structure, a 5 ' conserved sequence element, a subgenomic promoter, a3 ' conserved sequence element, and/or a poly a tail.
12. The mRNA of any one of claims 6 to 11, wherein the mRNA is a monocistronic, bicistronic, or polycistronic mRNA.
13. A composition comprising a protein according to any one of claims 1 to 5.
14. The composition of claim 13, further comprising an excipient.
15. A composition comprising the mRNA of any one of claims 6-12.
16. The composition of claim 15, further comprising a cationic or polycationic compound; preferably, the composition further comprises liposomes.
17. A nucleic acid encoding a protein according to any one of claims 1 to 5.
18. A vector comprising the nucleic acid of claim 17.
19. A cell comprising a protein according to any one of claims 1 to 5, a nucleic acid according to claim 17 and/or a vector according to claim 18.
20. A vaccine comprising a protein according to any one of claims 1 to 5, an mRNA according to any one of claims 6 to 12, a composition according to any one of claims 13 to 16, a nucleic acid according to claim 17, a vector according to claim 18 or a cell according to claim 19.
21. A method of preparing a composition or vaccine comprising a protein, said method comprising admixing a protein according to any one of claims 1-5 and an excipient.
22. A method for preparing a composition or vaccine comprising mRNA, the method comprising mixing the mRNA of any one of claims 6 to 12 with a cationic or polycationic compound and then packaging the mixture in liposomes.
23. Use of a protein according to any one of claims 1 to 5, an mRNA according to any one of claims 6 to 12, a composition according to any one of claims 13 to 16, a nucleic acid according to claim 17, a vector according to claim 18 or a cell according to claim 19 for the preparation of a medicament for the prophylaxis and/or treatment of a coronavirus infection of the type SARS-2-CoV.
CN202210055493.3A 2021-01-19 2022-01-18 Protein or protein composition, and preparation method and application thereof Pending CN114478798A (en)

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US20200061185A1 (en) * 2016-10-25 2020-02-27 The United States Of America, As Represented By The Secretary, Department Of Health And Human Servic Prefusion coronavirus spike proteins and their use
CN112076315A (en) * 2020-08-25 2020-12-15 中国农业科学院生物技术研究所 Nano antigen particle fused with new coronavirus S protein and ferritin subunit, new coronavirus vaccine, and preparation method and application thereof

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