CN114471890B - Grinding filter for preparing tissue single cells in handheld mode - Google Patents
Grinding filter for preparing tissue single cells in handheld mode Download PDFInfo
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- CN114471890B CN114471890B CN202111553889.2A CN202111553889A CN114471890B CN 114471890 B CN114471890 B CN 114471890B CN 202111553889 A CN202111553889 A CN 202111553889A CN 114471890 B CN114471890 B CN 114471890B
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- 238000000227 grinding Methods 0.000 title claims abstract description 121
- 239000004744 fabric Substances 0.000 claims abstract description 22
- 238000009434 installation Methods 0.000 claims description 8
- 230000006978 adaptation Effects 0.000 claims description 4
- 238000004113 cell culture Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 4
- 238000003801 milling Methods 0.000 description 4
- 238000005498 polishing Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 229920001169 thermoplastic Polymers 0.000 description 1
- 239000004416 thermosoftening plastic Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/08—Pestle and mortar
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C23/00—Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group
- B02C23/08—Separating or sorting of material, associated with crushing or disintegrating
- B02C23/14—Separating or sorting of material, associated with crushing or disintegrating with more than one separator
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C23/00—Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group
- B02C23/08—Separating or sorting of material, associated with crushing or disintegrating
- B02C23/16—Separating or sorting of material, associated with crushing or disintegrating with separator defining termination of crushing or disintegrating zone, e.g. screen denying egress of oversize material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/33—Disintegrators
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C23/00—Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group
- B02C23/08—Separating or sorting of material, associated with crushing or disintegrating
- B02C23/16—Separating or sorting of material, associated with crushing or disintegrating with separator defining termination of crushing or disintegrating zone, e.g. screen denying egress of oversize material
- B02C2023/165—Screen denying egress of oversize material
Landscapes
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a hand-held grinding filter for preparing tissue single cells, which comprises the following components: the grinding device comprises a grinding pipe and a grinding pestle, wherein a first screen is plugged at one end of the grinding pipe, a second screen is fixed in the grinding pipe, an annular plate is fixed on the outer wall of the grinding pipe, and a handle is fixed on the annular plate; the grinding pestle includes action bars and grinding head, and the action bars includes linkage segment and gripping section, and the linkage segment is arranged for gripping section slope, and the linkage segment is fixed with the grinding head, and the grinding head can keep away from one side web face butt of screen cloth one with screen cloth two. According to the grinding pipe, the annular plate is fixed on the outer wall of the grinding pipe, and the handle is fixed on the outer side wall of the annular plate, so that an operator can conveniently hold the grinding pipe; the operating lever includes the linkage segment and is the section of gripping of certain contained angle rather than being, and the linkage segment is fixed with the grinding head, and this setting makes operator's hand can not be directly over cultivateing the hole, effectively avoids operator's finger to drop in the grinding pipe.
Description
Technical Field
The invention relates to the field of biological experiment devices, in particular to a grinding filter for preparing tissue single cells in a handheld manner.
Background
The grinding method is adopted to prepare the single-cell suspension of animal tissues, so that the state of cells is influenced, the cells are easy to break due to excessive grinding, the probability of cell pollution is high, a user is inconvenient to operate the conventional grinding filter in the use process, the grinding pipe is generally in an open state, scraps on the hands of the operator can fall into the grinding pipe in the grinding process, the cell pollution is caused, the conventional filter can only be matched with a centrifuge tube, and tissue cell grinding operation cannot be performed on utensils such as a culture dish.
Therefore, how to provide a grinding filter for preparing tissue single cells in a handheld manner, so that the above problems can be overcome, is a problem that needs to be solved by those skilled in the art.
Disclosure of Invention
In view of this, the present invention provides a milling filter for preparing tissue single cells in a hand-held manner.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a hand-held abrasive filter for preparing tissue single cells, comprising:
The grinding device comprises a grinding pipe, wherein one end of the grinding pipe is plugged with a first screen, a second screen is fixed in the grinding pipe, the second screen is arranged parallel to the first screen, the second screen is larger than the first screen in mesh number, an annular plate is fixed on the outer wall of one end of the grinding pipe far away from the first screen, the annular plate is arranged parallel to the first screen, a handle is fixed on the outer side wall of the annular plate, and the handle is arranged perpendicular to the length direction of the grinding pipe;
The grinding pestle, the grinding pestle includes action bars and grinding head, the action bars include linkage segment and hold the section, the linkage segment for hold the section slope and arrange, the linkage segment keep away from hold the one end of section with the grinding head is fixed, the grinding head is kept away from the one end of linkage segment can with screen cloth second is kept away from one side guipure butt of screen cloth first.
Compared with the prior art, the invention discloses the grinding filter for preparing tissue single cells in a handheld mode, the annular plate is fixed on the outer wall of the grinding pipe, and the handle is fixed on the outer side wall of the annular plate, so that an operator can conveniently hold the grinding pipe; the operating lever includes the linkage segment and is the section of gripping of certain contained angle rather than being, and the linkage segment is fixed with the grinding head, and this setting makes the operator grind the operation through the section of gripping to the grinding head, makes the operator's hand can not be directly over cultivateing the hole, effectively avoids the operator's finger to drop the thing and drop to the grinding intraductal.
Preferably, a plurality of supporting blocks are uniformly distributed and fixed on the end wall of one end of the grinding pipe, on which the first screen is fixed. This setting makes this grind filter can use with the culture dish collocation, has further increased this adaptation application range who grinds the filter.
Preferably, the grinding device further comprises a connecting ring, wherein the section of the grinding pipe is circular, the connecting ring is coaxially sleeved and fixed on the outer wall of the grinding pipe, and the outer wall of the connecting ring is simultaneously fixed with a side plate surface, close to the first screen, of the annular plate. This setting can improve the joint strength of annular plate and grinding pipe to ensure that grinding pipe inserts when establishing to cultivateing downthehole portion, can not take place to rock by a wide margin.
Preferably, the connecting section is kept away from the one end integrated into one piece between two parties of section of gripping has a circular shape backup pad, the backup pad perpendicular to the connecting section is arranged, the backup pad is kept away from one side face of connecting section is inwards sunken out a installation blind hole in the middle, the hole outer fringe diameter of installation blind hole is less than its downthehole footpath, the grinding head wholly be cylindrical and its one end integrated into one piece between two parties have one with the connector of installation blind hole adaptation, the connector joint is in the installation blind hole, the external diameter of grinding head is greater than or equal to the external diameter of backup pad. This arrangement ensures that the grinding bit can be reliably secured to the connection section.
Preferably, the outer wall of the holding section is provided with anti-slip lines. This arrangement facilitates the operator to hold the lever.
Preferably, the length of the connecting section is smaller than that of the holding section, and the included angle between the connecting section and the holding section is 120-150 degrees. This setting helps improving the comfort level when the operator operates the grinding head on the one hand, and on the other hand makes the operator's hand can not be directly over cultivateing the hole, effectively avoids the operator's finger to drop in the grinding pipe.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
FIG. 1 is an overall isometric view of a hand-held abrasive filter for preparing tissue single cells;
FIG. 2 is a second perspective view of a milling filter for hand-held preparation of tissue single cells;
FIG. 3 is a partial isometric view of a hand-held abrasive filter for preparing tissue single cells;
FIG. 4 is a partial isometric view of a milling filter for hand-held preparation of tissue single cells;
FIG. 5 is a top view of a milling filter for hand-held preparation of tissue single cells;
fig. 6 is a cross-sectional view in the direction of fig. 5B-B.
In the figure:
the grinding device comprises a grinding pipe 1, a screen I2, a screen II 3, an annular plate 4, a handle 5, an operating rod 6, a connecting section 60, a holding section 61, a grinding head 7, a supporting block 8, a connecting ring 9, a supporting plate 10, a blind mounting hole 100 and a connecting head 11.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention discloses a grinding filter for preparing tissue single cells in a handheld manner, which is characterized in that an annular plate 4 is fixed on the outer wall of a grinding tube 1, and a handle 5 is fixed on the outer side wall of the annular plate 4, so that an operator can conveniently hold the grinding tube 1; the operating rod 6 comprises a connecting section 60 and a holding section 61 which forms a certain included angle with the connecting section 60, and the connecting section 60 is fixed with the grinding head 7, so that an operator can grind the grinding head 7 through the holding section 61, the hand of the operator can not be directly above the culture hole, and falling of the finger falling object of the operator into the grinding tube 1 is effectively avoided; through be fixed with a plurality of supporting shoes 8 of one end wall of screen cloth one 2 at grinding pipe 1, this setting makes this grinding filter can use with the culture dish collocation, has further increased this grinding filter's adaptation application range.
Examples
Referring to fig. 1-6, which are schematic views of the whole and part of the structure of an embodiment of the present invention, the present invention specifically discloses a grinding filter for preparing single cells of a tissue in a hand-held manner, which needs to be used in combination with a cell culture plate (not shown in the figure) or a culture dish (not shown in the figure), wherein the cell culture plate may be a 6-well plate or a 12-well plate, and the grinding filter comprises:
The grinding tube 1, one end integrated into one piece of grinding tube 1 has screen cloth one 2, the mesh of screen cloth one 2 is perpendicular with the pipe length direction of grinding tube 1, the inside screen cloth two 3 that is fixed with a metal material of grinding tube 1, screen cloth two 3 are arranged parallel to screen cloth one 2 and have the clearance between the two, the clearance between screen cloth two 3 and the screen cloth one 2 is less than 2mm, the mesh number of screen cloth two 3 is greater than the mesh number of screen cloth one 2, the aperture control of screen cloth one 2 is 1 mm-2.5 mm, the mesh number of screen cloth two 3 is 200 mesh-400 mesh, integrated into one piece has a ring plate 4 on the one end outer wall that grinding tube 1 kept away from screen cloth one 2, ring plate 4 is arranged parallel to screen cloth one 2 and integrated into one piece has a handle 5 on its lateral wall, handle 5 perpendicular to the pipe length direction of grinding tube 1 is arranged. The length of the grinding tube 1 is smaller than the hole depth of a culture hole on a cell culture plate matched with the grinding tube 1, the outer diameter of the grinding tube 1 is smaller than the hole diameter of the culture hole, the hole diameter difference of the two is 1mm, the wall thickness of the grinding tube 1 is 1mm, the outer diameter of the annular plate 4 is larger than the hole diameter of the culture hole, the inner diameter of the annular plate 4 is smaller than the hole diameter of the culture hole, the hole diameter difference of the two is at least more than 6 mm, the arrangement ensures that the grinding tube 1 can be inserted into the culture hole, and the grinding tube 1 can be erected on the culture hole through the annular plate 4, so that a certain interval distance is kept between a filter screen I and the bottom of the culture hole;
The grinding pestle, grind the pestle and include action bars 6 and grinding bit 7, the action bars 6 include that the cross-section is circular shape linkage segment 60 and with linkage segment 60 integrated into one piece hold the segment 61, hold segment 61 along with the further its cross-section diameter of linkage segment 60 is bigger, linkage segment 60 is for holding segment 61 slope arrangement, the one end that linkage segment 60 kept away from holding segment 61 is fixed with grinding bit 7, the grinding bit 7 that adopts the elastic material preparation is kept away from the one end that linkage segment 60 can keep away from one side mesh side butt of screen cloth 2 with screen cloth two 3.
Further specifically, four supporting blocks 8 are uniformly distributed on the end wall of one end of the grinding pipe 1, where the first screen 2 is fixed, the heights of the four supporting blocks 8 are 2mm, and when the grinding filter is matched with the culture dish for use, the four supporting blocks 8 can support the grinding pipe 1, so that a proper interval is reserved between the first screen 2 and the inner bottom wall of the culture dish. The grinding tube 1, the first screen 2, the annular plate 4, the operating rod 6 and the supporting block 8 are all made of thermoplastic transparent resin materials.
Further specifically, the novel cell culture device further comprises a connecting ring 9, the outer contour of the connecting ring 9 is circular, the section of the connecting ring 9 is rectangular, the outer diameter of the connecting ring 9 is slightly smaller than the aperture of a culture hole in a cell culture plate matched with the connecting ring, so that the grinding tube 1 can be prevented from shaking in the culture hole during grinding, the section of the grinding tube 1 is circular, the connecting ring 9 is coaxially fixed on the outer wall of the grinding tube 1 in a sleeved mode, and the outer wall of the connecting ring is simultaneously fixed with a side plate surface of the annular plate 4, which is close to the first screen 2.
Further specifically, one end of the connecting section 60, which is far away from the holding section 61, is centrally integrated into one piece with a circular support plate 10, the support plate 10 is arranged perpendicular to the connecting section 60, one side plate surface of the support plate 10, which is far away from the connecting section 60, is centrally and inwards recessed with a mounting blind hole 100, the diameter of the outer edge of the hole of the mounting blind hole 100 is smaller than the inner diameter of the hole, the grinding head 7 is integrally cylindrical, one end of the grinding head 7 is centrally integrated into one piece with a connector 11 which is matched with the mounting blind hole 100, the connector 11 is clamped in the mounting blind hole 100, and the outer diameter of the grinding head 7 is larger than or equal to the outer diameter of the support plate 10.
Further specifically, the outer wall of the grip section 61 is provided with anti-slip lines.
More specifically, the length of the connecting section 60 is less than the length of the gripping section 61 and the angle therebetween is 120 ° -150 °.
The using method of the grinding filter comprises the following steps:
when the grinding filter is matched with a cell culture plate for use, firstly, an operator holds the handle 5 by one hand, inserts the grinding tube 1 into the culture hole, puts the tissue to be ground into the grinding tube 1 after the insertion is finished, and holds the operating rod 6 by the other hand after the insertion is finished, and starts the grinding operation;
When the polishing filter is matched with a culture dish, firstly, an operator holds the handle 5 by one hand, vertically places the polishing tube 1 on the culture dish, ensures that the four supporting blocks 8 are all abutted with the inner bottom wall of the culture dish, then places the tissue to be polished into the polishing tube 1, and after the placing is completed, the operator holds the operating rod 6 by the other hand, and starts polishing operation.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (4)
1. A hand-held abrasive filter for preparing tissue single cells, comprising:
The grinding device comprises a grinding pipe (1), wherein a first screen (2) is plugged at one end of the grinding pipe (1), a second screen (3) is fixed inside the grinding pipe (1), the second screen (3) is parallel to the first screen (2), the mesh number of the second screen (3) is larger than that of the first screen (2), an annular plate (4) is fixed on the outer wall of one end, far away from the first screen (2), of the grinding pipe (1), the annular plate (4) is parallel to the first screen (2), a handle (5) is fixed on the outer side wall of the annular plate, and the handle (5) is perpendicular to the pipe length direction of the grinding pipe (1);
The grinding pestle comprises an operating rod (6) and a grinding head (7), wherein the operating rod (6) comprises a connecting section (60) and a holding section (61), the connecting section (60) is obliquely arranged relative to the holding section (61), one end of the connecting section (60) away from the holding section (61) is fixed with the grinding head (7), and one end of the grinding head (7) away from the connecting section (60) can be abutted with one side of the screen cloth II (3) away from the screen cloth I (2);
A plurality of supporting blocks (8) are uniformly distributed and fixed on the end wall of one end of the grinding pipe (1) where the first screen (2) is fixed; the four supporting blocks (8) can support the grinding tube (1) so that a proper interval is formed between the first screen (2) and the inner bottom wall of the culture dish;
The utility model discloses a grinding head, including connecting section (60), connecting section (61), connecting section (60) are kept away from one end integrated into one piece in the middle has a circular shape backup pad (10), backup pad (10) perpendicular to connecting section (60) are arranged, backup pad (10) are kept away from one side face of connecting section (60) is inwards sunken out one installation blind hole (100) in the middle, the hole outer fringe diameter of installation blind hole (100) is less than its downthehole footpath, grinding head (7) wholly be cylindrically and its one end integrated into one piece in the middle have one with connector (11) of installation blind hole (100) adaptation, connector (11) joint is in installation blind hole (100), the external diameter of grinding head (7) is greater than or equal to the external diameter of backup pad (10).
2. The grinding filter for preparing tissue single cells in a handheld manner according to claim 1, further comprising a connecting ring (9), wherein the section of the grinding tube (1) is circular, the connecting ring (9) is coaxially fixed on the outer wall of the grinding tube (1) in a sleeved manner, and the outer wall of the connecting ring is simultaneously fixed with the annular plate (4) close to a side surface of the screen one (2).
3. The grinding filter for preparing tissue single cells in a hand-held manner according to claim 1, wherein the outer wall of the holding section (61) is provided with anti-slip lines.
4. A grinding filter for preparing single cells of tissue in a hand-held manner according to claim 1, characterized in that the length of the connecting section (60) is smaller than the length of the holding section (61) and the angle between them is 120 ° -150 °.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202111553889.2A CN114471890B (en) | 2021-12-17 | 2021-12-17 | Grinding filter for preparing tissue single cells in handheld mode |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202111553889.2A CN114471890B (en) | 2021-12-17 | 2021-12-17 | Grinding filter for preparing tissue single cells in handheld mode |
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CN114471890A CN114471890A (en) | 2022-05-13 |
CN114471890B true CN114471890B (en) | 2024-05-24 |
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CN118460349A (en) * | 2024-07-10 | 2024-08-09 | 南昌大学第一附属医院 | Grinding device before preparation of bacterium fungus liquid |
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CN204841879U (en) * | 2015-08-03 | 2015-12-09 | 刘艳 | Filtration formula surgery grinds medicine ware |
CN209985537U (en) * | 2019-05-22 | 2020-01-24 | 青岛金域医学检验所有限公司 | Special tissue grinder of flow cytometry |
CN210097889U (en) * | 2019-04-23 | 2020-02-21 | 黄埔出入境检验检疫局综合技术服务中心 | Micro-tissue grinding device without rotating tube |
CN112044575A (en) * | 2020-09-02 | 2020-12-08 | 天马(安徽)国药科技股份有限公司 | Processing method and equipment suitable for superfine astragalus membranaceus powder beverage |
CN212215802U (en) * | 2019-10-16 | 2020-12-25 | 黄敏 | Novel chemical raw material grinding structure |
CN216631104U (en) * | 2021-12-17 | 2022-05-31 | 西安医学院 | Hand-held type preparation tissue unicell is used grinds filter |
-
2021
- 2021-12-17 CN CN202111553889.2A patent/CN114471890B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN204841879U (en) * | 2015-08-03 | 2015-12-09 | 刘艳 | Filtration formula surgery grinds medicine ware |
CN210097889U (en) * | 2019-04-23 | 2020-02-21 | 黄埔出入境检验检疫局综合技术服务中心 | Micro-tissue grinding device without rotating tube |
CN209985537U (en) * | 2019-05-22 | 2020-01-24 | 青岛金域医学检验所有限公司 | Special tissue grinder of flow cytometry |
CN212215802U (en) * | 2019-10-16 | 2020-12-25 | 黄敏 | Novel chemical raw material grinding structure |
CN112044575A (en) * | 2020-09-02 | 2020-12-08 | 天马(安徽)国药科技股份有限公司 | Processing method and equipment suitable for superfine astragalus membranaceus powder beverage |
CN216631104U (en) * | 2021-12-17 | 2022-05-31 | 西安医学院 | Hand-held type preparation tissue unicell is used grinds filter |
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