CN114471209A - Seed of virus diluting device of pseudorabies virus bacterin research - Google Patents
Seed of virus diluting device of pseudorabies virus bacterin research Download PDFInfo
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- CN114471209A CN114471209A CN202210137552.1A CN202210137552A CN114471209A CN 114471209 A CN114471209 A CN 114471209A CN 202210137552 A CN202210137552 A CN 202210137552A CN 114471209 A CN114471209 A CN 114471209A
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Abstract
The invention discloses a virus seed diluting device for researching pseudorabies virus vaccines, and particularly relates to the technical field of virus seed dilution, which comprises a diluting box, wherein a protective door is arranged on the front side of the diluting box, one side of the protective door is fixedly connected with a control button on the position of the diluting box through a bolt, and a diluting mechanism is arranged above the diluting box; the dilution mechanism holds the case including setting up the washing liquid in dilution case top, washing liquid holds case one side and installs and wait to evaluate antigen and hold the case, wait to evaluate antigen and hold case one side and install an anti diluent and hold the case and be provided with two anti diluents and hold the case in an anti diluent holds case one side. The invention adopts the diluting mechanism, each diluent can not cause dripping errors, automatic metering operation is realized in the dripping process, the dripping is more accurate, the diluting accuracy in the research process is greatly improved, the diluting accuracy is improved, and the use is convenient.
Description
Technical Field
The invention relates to the technical field of virus seed dilution, in particular to a virus seed dilution device for pseudorabies virus vaccine research.
Background
The pseudorabies virus is also called as a swine herpesvirus type I, an infectious bulbar paralysis virus, a severe itching syndrome virus and an Orientski virus, is a herpesvirus which causes fever, severe itching (except for pigs) and encephalomyelitis of various domestic animals such as cattle, sheep, pigs, dogs, cats and the like and wild animals as main symptoms, and is named as pseudorabies because the clinical symptoms of the pseudorabies virus are similar to rabies, and in the research process of a pseudorabies vaccine, the virus seeds need to be diluted, so a diluting device is needed to operate.
In actual use, a person is required to dilute the virus seeds once and once, and the diluting substances added in different time periods are different in the diluting process, so that the diluting sequence is disordered and the diluting effect is influenced during dilution, and the operation is very inconvenient.
Disclosure of Invention
In order to overcome the defects in the prior art, the embodiment of the invention provides a virus seed diluting device for researching pseudorabies virus vaccines, and the device adopts a diluting mechanism, so that various diluents can be switched to be dripped back and forth, dripping errors cannot be caused by each diluent, automatic metering operation is realized in the dripping process, dripping is more accurate, the diluting accuracy in the research process is greatly improved, the diluting accuracy is improved, the use is convenient, and the problems in the background art are solved.
In order to achieve the purpose, the invention provides the following technical scheme: a virus seed diluting device for pseudorabies virus vaccine research comprises a diluting box, wherein a protective door is arranged on the front side of the diluting box, a control button on the position of the diluting box is fixedly connected to one side of the protective door through a bolt, and a diluting mechanism is arranged above the diluting box;
diluting the mechanism and bearing the vanning including the washing liquid that sets up in dilution case top, washing liquid bears vanning one side and installs and treat that the evaluation antigen holds the vanning, treat that the evaluation antigen holds vanning one side and installs an anti diluent and hold the vanning and be provided with two anti diluents in an anti diluent holds vanning one side, washing liquid holds the vanning, treats that the evaluation antigen holds the vanning, an anti diluent holds the vanning, two anti diluents hold the vanning top and all install the pump machine, has coupling hose in pump machine output intercommunication, the flowmeter is installed to the coupling hose bottom, the flowmeter below is provided with switching component.
In a preferred embodiment, the switching component includes a washing liquid injection tube disposed at an input end of the flow meter, a to-be-evaluated liquid injection tube is mounted on one side of the washing liquid injection tube, an anti-dilution injection tube is disposed on one side of the to-be-evaluated liquid injection tube, a secondary antibody dilution injection tube is mounted on one side of the anti-dilution injection tube, four linkage rotating rods are disposed in gaps formed among the washing liquid injection tube, the to-be-evaluated liquid injection tube, the anti-dilution injection tube and the secondary antibody dilution injection tube, a rotating motor is mounted at one end of each linkage rotating rod, a pressing cylinder is disposed above each rotating motor, a sleeving moving block is connected to one side of each pressing cylinder, and a driving screw is in threaded connection with an inner wall of each sleeving moving block.
In a preferred embodiment, a sleeving frame is installed outside the sleeving moving block, a first driving motor is installed at one end of the driving screw, the first driving motor and the driving screw are in coaxial transmission connection, and two heating resistance tubes are installed on the inner wall of the dilution box.
In a preferred embodiment, the washing liquid containing box, the antigen containing box to be evaluated, the primary anti-dilution liquid containing box and the secondary anti-dilution liquid containing box are arranged in an annular and equidistant distribution manner, and the connecting hose is communicated with the flowmeter.
In a preferred embodiment, washing liquid injection pipe installs link gear in the below, link gear is including setting up the first location orifice plate in washing liquid injection pipe below, first location orifice plate one side is connected with a socket joint section of thick bamboo through the bolt is detachable, socket joint section of thick bamboo internally mounted has first actuating lever, first drive motor is installed to first actuating lever one end and installs the second actuating lever at the first actuating lever other end, the second drive motor that coaxial drive connects is installed to second actuating lever one end, the second actuating lever outside just is located and cup joints a section of thick bamboo and second drive motor between position department cover and is equipped with the cover and connect the piece, and is connected with second location orifice plate in the department of cup jointing a piece one side position, the second actuating lever outside just is located and cup joints a piece other side position department cover and is equipped with from the heart frame.
In a preferred embodiment, a centrifugal support rod is embedded in the bottom end of the inner wall of the centrifugal frame, a second driving motor is installed at a position below the centrifugal support rod, a decolorizing disc is connected to the outer wall of the second driving motor, and two vibrating motors are arranged on one side of the decolorizing disc.
In a preferred embodiment, the first driving rod is fixedly connected with the socket sleeve, and the second driving rod is welded and fixed with the inner part of the socket sleeve.
In a preferred embodiment, the inner wall of the top end of the centrifugal frame is movably connected with the outside of the second driving rod, and the outer wall of the sleeving block is detachably connected with the second positioning hole plate through a bolt.
In a preferred embodiment, a supporting annular plate is installed on one side of the dilution box, a rotary rod is installed on one side of the supporting annular plate in an embedded mode, a servo motor is connected to one end of the rotary rod in a coaxial transmission mode, a rotary disk is installed on the other end of the rotary rod, four linkage supporting blocks are distributed on one side of the rotary disk in an annular equal distance mode, an annular shaft rod is arranged inside each linkage supporting block, a positioning fixture block is connected to one side of a limiting spiral ring in an embedded mode, a washing liquid containing box is installed at the position of one side of the positioning fixture block, an antigen recovery box to be evaluated is arranged below the washing liquid containing box, a primary antibody recovery box is installed on one side of the antigen recovery box to be evaluated, and a secondary antibody recovery box is installed at the position of the lower portion of the primary antibody recovery box.
In a preferred embodiment, one end of the rotating rod penetrates through one side of the supporting collar plate and extends to the other side of the supporting collar plate, the outside of the rotating rod is movably connected with the inside of the supporting collar plate, the outside of the collar shaft rod is movably connected with the coaxial bearing inside the linkage support block, a position, located on one side of the linkage support block, of the outside of the collar shaft rod is in threaded connection with a limiting spiral ring, and a support block is installed below the servo motor.
The invention has the technical effects and advantages that:
1. the invention adopts the dilution mechanism to drive the linkage rotating rod to rotate ninety degrees clockwise, so that the linkage rotating rod drives the washing liquid injection tube to rotate and is positioned above the first positioning hole plate, the washing liquid in the washing liquid containing box enters the connecting hose and enters the flowmeter along the connecting hose to be counted, the washing liquid injection tube moves downwards to the upper surface of the first positioning hole plate, the linkage rotating rod is driven by the rotating motor to rotate ninety degrees clockwise, so that the evaluation liquid injection tube is positioned above the second positioning hole plate, the rotating motor is started to drive the anti-dilution injection tube to rotate continuously, so that the anti-dilution injection tube is positioned at the upper surface of the first positioning hole plate, the linkage rotating rod is driven by the rotating motor to rotate ninety degrees continuously, and the anti-dilution liquid in the anti-dilution liquid containing box can be injected into the anti-dilution injection tube, therefore, the dripping can be switched back and forth for various diluents, each diluent cannot cause dripping errors, automatic metering operation is realized in the dripping process, the dripping is more accurate, the diluting accuracy in the research process is greatly improved, the diluting accuracy is improved, and the use is convenient;
2. the invention adopts a linkage mechanism to drive a first driving rod to rotate thirty-five degrees clockwise by a first driving motor, a sleeve joint cylinder drives a first positioning pore plate to rotate and incline, a second driving motor is started to drive a second driving rod to rotate thirty-five degrees anticlockwise, a sleeve joint block drives a second positioning pore plate to rotate and incline, coating liquid in the second positioning pore plate and the first positioning pore plate is discarded, a second driving motor in a decoloring disc rotates, the second driving motor drives a centrifugal support rod to rotate, the first positioning pore plate and the second positioning pore plate can be spin-dried, so that the operation of obliquely discarding the coating liquid can be realized in the diluting process, meanwhile, the excess coating liquid can be dried in a drying way, so that the integrated operation is realized, the discarding efficiency is greatly improved, the drying operation is improved, and the better removal effect of the excess diluted liquid is achieved;
3. when the invention needs to collect the washing liquid waste water, the washing liquid waste water enters the washing liquid containing box to complete the loading operation, when the antigen to be evaluated is, the servo motor can be started to drive the rotating rod to rotate for ninety degrees, the washing liquid containing box is always vertically downward under the action of gravity, the antigen recovery box to be evaluated is butted at the position below the diluting box to collect the antigen diluting liquid to be evaluated, the servo motor drives the rotating rod to continue rotating, the secondary antibody recycling box is positioned at the lower position of the diluting box and continuously rotates, so that the primary antibody recycling box is positioned at the lower position of the diluting box to carry and load the primary antibody diluting solution, therefore, different diluents can be subjected to waste liquid distinguishing and collecting, various mixed liquids are prevented from being gathered together, and classification cannot be performed, so that the waste liquid is more conveniently treated in the later period, and the effect of safe classification is achieved;
to sum up, through the mutual influence of above-mentioned a plurality of structures, can realize that multiple diluent can make a round trip to switch the dropwise add, every kind of diluent can not cause the dropwise add mistake like this, and the dropwise add in-process, realize the automatic measure operation, the dropwise add is more accurate, realize the integration operation, not only increased substantially and abandoned efficiency and improved the operation of spin-drying, reach better unnecessary diluent and get rid of the operation, different diluents carry out the waste liquid and distinguish the collection, avoid causing various mixed liquids to converge together, can't carry out classification, make things convenient for the later stage to the processing of waste liquid more, reach safe categorised effect, can effectively improve in diluting the-process in sum, high accuracy and avoid the dropwise add mistake, lead to the dilution effect poor, can improve the classification to the waste liquid in later stage simultaneously, and is more environment-friendly.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a schematic side-view perspective structure of the present invention.
FIG. 3 is a schematic view of the internal structure of the dilution box of the present invention.
FIG. 4 is a schematic view of the structure of the connection between the washing liquid injection tube and the flow meter.
FIG. 5 is a schematic view of the structure of the connection between the centrifugal support rod and the centrifugal frame.
Fig. 6 is a sectional view of the first driving rod according to the present invention.
Fig. 7 is a schematic structural diagram of a connection part of a rotating rod and a servo motor according to the present invention.
FIG. 8 is a schematic view of the joint between the linking support and the shaft of the collar.
The reference signs are: 1. a dilution tank; 2. a protective door; 3. a control button; 4. a washing liquid containing box; 5. carrying and packing the antigen to be evaluated; 6. an anti-diluent containing box; 7. loading the secondary antibody diluent into a box; 8. a pump machine; 9. a connecting hose; 10. a flow meter; 11. a washing solution injection tube; 12. a to-be-evaluated liquid injection tube; 13. an anti-dilution syringe; 14. a secondary antibody dilution syringe; 15. a linkage rotating rod; 16. a rotating electric machine; 17. pressing down the air cylinder; 18. sleeving a moving block; 19. a drive screw; 20. sleeving and connecting the frame; 21. a first drive motor; 22. a first positioning orifice plate; 23. sleeving a sleeve; 24. a first drive lever; 25. a first drive motor; 26. a second drive lever; 27. sleeving and connecting blocks; 28. a second drive motor; 29. a second positioning orifice plate; 30. a centrifuge frame; 31. a centrifugal strut; 32. a second drive motor; 33. a decolorizing plate; 34. a vibration motor; 35. supporting the collar plate; 36. rotating the rod; 37. a servo motor; 38. rotating the disc; 39. linkage supporting blocks; 40. a collar shaft; 41. positioning a fixture block; 42. a cleaning solution storage box; 43. an antigen recovery tank to be evaluated; 44. a first resistance recovery box; 45. a secondary antibody recovery tank; 46. a limiting spiral ring; 47. a support block; 48. the resistance tube is heated.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The virus seed diluting device for the research of the pseudorabies virus vaccine shown in the attached figures 1-8 comprises a diluting box 1, wherein a protective door 2 is arranged on the front side of the diluting box 1, a control button 3 on the position of the diluting box 1 is fixedly connected to one side of the protective door 2 through a bolt, and a diluting mechanism is arranged above the diluting box 1;
dilution mechanism holds vanning 4 including the washing liquid that sets up in dilution case 1 top, washing liquid holds vanning 4 one side and installs and holds vanning 5 of waiting to evaluate antigen, it holds vanning 6 and is provided with two anti dilution holding case 7 in an anti dilution holding case 6 one side to wait to evaluate antigen, washing liquid holds vanning 4, it holds vanning 5 to wait to evaluate antigen, an anti dilution holding case 6, pump machine 8 is all installed on two anti dilution holding case 7 tops, 8 output intercommunication have coupling hose 9 at pump machine, flowmeter 10 is installed to coupling hose 9 bottom, flowmeter 10 below is provided with switching component.
In some embodiments, as shown in the drawings, the switching component includes a washing liquid injection tube 11 disposed at an input end of the flow meter 10, a to-be-evaluated liquid injection tube 12 is mounted at one side of the washing liquid injection tube 11, an anti-dilution injection tube 13 is disposed at one side of the to-be-evaluated liquid injection tube 12, a secondary antibody dilution injection tube 14 is mounted at one side of the primary antibody dilution injection tube 13, four linkage rotating rods 15 are disposed in a gap formed between the washing liquid injection tube 11, the to-be-evaluated liquid injection tube 12, the primary anti-dilution injection tube 13, and the secondary antibody dilution injection tube 14, a rotating motor 16 is mounted at one end of the linkage rotating rods 15, a pressing cylinder 17 is disposed above the rotating motor 16, a sleeve moving block 18 is connected at one side of the pressing cylinder 17, a driving screw 19 is connected to an inner wall of the sleeve moving block 18 through a screw thread, a sleeve frame 20 is mounted outside the sleeve moving block 18, a first driving motor 21 is mounted at one end of the driving screw 19, the first driving motor 21 is coaxially connected with the driving screw rod 19 in a transmission way, the inner wall of the dilution box 1 is provided with two heating resistance tubes 48, the washing liquid containing box 4, the antigen containing box 5 to be evaluated, the primary anti-dilution liquid containing box 6 and the secondary anti-dilution liquid containing box 7 are annularly and equidistantly distributed, and the connecting hose 9 is communicated with the flowmeter 10;
so that, the rotary motor 16 is started to drive the linkage rotary rod 15 to rotate ninety degrees clockwise, so that the linkage rotary rod 15 drives the washing liquid injection tube 11 to rotate and is positioned above the first positioning orifice plate 22, the pump 8 is started, so that the washing liquid in the washing liquid bearing box 4 enters the connecting hose 9 and enters the flow meter 10 along the connecting hose 9 for counting, meanwhile, the pressing cylinder 17 is started to drive the rotary motor 16 to move downwards, so that the rotary motor 16 drives the linkage rotary rod 15 to move the washing liquid injection tube 11 downwards to the upper surface of the first positioning orifice plate 22, then the first driving motor 21 is started to drive the driving screw rod 19 to rotate forwards, the driving screw rod 19 drives the sleeving movable block 18 to move leftwards under the action of threads, so that each injection head of the washing liquid injection tube 11 is injected into each hole position of the first positioning orifice plate 22 and each injection head of the second positioning orifice plate 29, each hole is 100 mu L, the antigen to be evaluated is added, the rotating motor 16 is started to drive the linkage rotating rod 15 to rotate clockwise by ninety degrees, and primary antibody is added: the rotating motor 16 can be started to drive the primary anti-dilution injection tube 13 to continue rotating, and the enzyme-labeled secondary antibody is added: the rotating motor 16 can be started to drive the linkage rotating rod 15 to rotate ninety degrees continuously, and the effect of sequential addition is realized.
In some embodiments, as shown in the drawings, a linkage mechanism is installed below the washing liquid injection tube 11, the linkage mechanism includes a first positioning orifice plate 22 disposed below the washing liquid injection tube 11, one side of the first positioning orifice plate 22 is detachably connected with a socket 23 through a bolt, a first driving rod 24 is installed inside the socket 23, one end of the first driving rod 24 is installed with a first driving motor 25, the other end of the first driving rod 24 is installed with a second driving rod 26, one end of the second driving rod 26 is installed with a second driving motor 28 in coaxial transmission connection, a socket block 27 is sleeved outside the second driving rod 26 and located between the socket 23 and the second driving motor 28, a second positioning orifice plate 29 is connected at one side of the socket block 27, and a centrifugal frame 30 is sleeved outside the second driving rod 26 and located at the other side of the socket block 27;
a centrifugal support rod 31 is embedded and mounted at the bottom end of the inner wall of the centrifugal frame 30, a second driving motor 32 is mounted at a position below the centrifugal support rod 31, the outer wall of the second driving motor 32 is connected with a decolorizing disc 33, two vibrating motors 34 are arranged on one side of the decolorizing disc 33, the outer part of the first driving rod 24 is fixedly connected with the sleeve joint barrel 23, the outer part of the second driving rod 26 is fixedly welded with the inner part of the sleeve joint block 27, the inner wall of the top end of the centrifugal frame 30 is movably connected with the outer part of the second driving rod 26, and the outer wall of the sleeve joint block 27 is detachably connected with the second positioning hole plate 29 through bolts;
so, first drive motor 25 drives first actuating lever 24 and carries out thirty-five degrees of clockwise rotation, first actuating lever 24 drives a socket 23 and rotates, socket 23 drives first locating hole board 22 and rotates the slope, second drive motor 28 drives second actuating lever 26 and carries out thirty-five degrees of anticlockwise rotation, cover joint piece 27 drives second locating hole board 29 and rotates the slope, make the inside peridium liquid of second locating hole board 29 and first locating hole board 22 discard, second drive motor 32 drives centrifugal strut 31 and rotates, centrifugal strut 31 drives centrifugal frame 30 and rotates, centrifugal frame 30 drives second actuating lever 26 and first actuating lever 24 and rotates, can make first locating hole board 22 and second locating hole board 29 rotate and spin-dry, and can start vibrating motor 34 and drive decolorizing dish 33 and vibrate the decoloration.
In some embodiments, as shown in the drawings, a supporting collar plate 35 is installed on one side of a dilution tank 1, a rotating rod 36 is installed on one side of the supporting collar plate 35 in an embedded manner, one end of the rotating rod 36 is coaxially connected with a servo motor 37 in a transmission manner, a rotating disk 38 is installed on the other end of the rotating rod, four linkage support blocks 39 are annularly and equidistantly distributed on one side of the rotating disk 38, a collar shaft lever 40 is arranged inside each linkage support block 39, a positioning fixture block 41 is embedded and clamped on one side of a limiting spiral ring 46, a washing liquid containing box 42 is installed at one side of the positioning fixture block 41, an antigen recovery tank 43 to be evaluated is arranged below the washing liquid containing box 42, an anti-recovery tank 44 is installed on one side of the antigen recovery tank 43 to be evaluated, and an anti-recovery tank 45 is installed at a position below the anti-recovery tank 44;
one end of the rotating rod 36 penetrates through one side of the supporting collar plate 35 and extends to the other side of the supporting collar plate 35, the outside of the rotating rod 36 is movably connected with the inside of the supporting collar plate 35, the outside of the collar shaft lever 40 is movably connected with a coaxial bearing inside the linkage supporting block 39, the position, located on one side of the linkage supporting block 39, of the outside of the collar shaft lever 40 is in threaded connection with a limiting screw ring 46, and a supporting block 47 is installed below the servo motor 37;
when the washing liquid wastewater needs to be collected, the washing liquid wastewater can enter the washing liquid containing box 42 to complete the bearing operation, the servo motor 37 can be started to drive the rotating rod 36 to rotate by ninety degrees, the washing liquid containing box 42 can drive the positioning fixture block 41 to rotate, the positioning fixture block 41 drives the linkage support block 39 to rotate outside the lantern ring shaft rod 40, and the washing liquid containing box 42 is always vertically downward under the action of gravity, so that the antigen recovery box 43 to be evaluated is butted at the lower position of the dilution box 1 to continuously collect the antigen to be evaluated, then the servo motor 37 can be started to drive the rotating rod 36 to continuously rotate, the secondary antibody recovery box 45 is positioned at the lower position of the dilution box 1 to collect the secondary antibody diluent, and the secondary antibody recovery box 44 can be positioned at the lower position of the dilution box 1 to carry out the bearing operation on the primary antibody diluent by continuous rotation, and the support block 47 can play a supporting role on the servo motor 37, the limiting screw ring 46 can limit the linkage support block 39.
The working principle of the invention is as follows: coating antibody: placing antibody pig serum into the first positioning pore plate 22 and the second positioning pore plate 29 in sequence, coating the antibody pig serum with coating liquid, diluting the antibody pig serum with the final concentration of 0.2 mu g/mL, coating 50 mu L of antibody pig serum in each hole into the first positioning pore plate 22 and the second positioning pore plate 29, heating the antibody pig serum to 37 ℃ by using a heating resistance tube 48, and standing the antibody pig serum for 2 hours.
During washing: the first driving motor 25 is started to drive the first driving rod 24 to rotate thirty-five degrees clockwise, the first driving rod 24 drives the sleeve connecting cylinder 23 to rotate, the sleeve connecting cylinder 23 drives the first positioning orifice plate 22 to rotate and incline, meanwhile, the second driving motor 28 is started to drive the second driving rod 26 to rotate thirty-five degrees anticlockwise, the second driving rod 26 drives the sleeve connecting block 27 to rotate, the sleeve connecting block 27 drives the second positioning orifice plate 29 to rotate and incline, so that the coating liquid in the second positioning orifice plate 29 and the first positioning orifice plate 22 can be discarded, then the second driving motor 32 in the decoloring disc 33 is started to rotate, the second driving motor 32 drives the centrifugal support rod 31 to rotate, the centrifugal support rod 31 drives the centrifugal frame 30 to rotate, the centrifugal frame 30 drives the second driving rod 26 and the first driving rod 24 to rotate, and the first positioning orifice plate 22 and the second positioning orifice plate 29 can be spin-dried, after the spin-drying is finished, the rotating motor 16 can be started to drive the linkage rotating rod 15 to rotate ninety degrees clockwise, so that the linkage rotating rod 15 drives the washing liquid injection tube 11 to rotate and is positioned above the first positioning hole plate 22, then the second transmission motor 28 drives the second driving rod 26 to rotate clockwise, and the first transmission motor 25 drives the first driving rod 24 to rotate thirty-five degrees anticlockwise;
thus, the first positioning orifice plate 22 and the second positioning orifice plate 29 can be reset and parallel, then the pump 8 is started, so that the washing liquid in the washing liquid containing box 4 enters the connecting hose 9 and enters the flowmeter 10 along the connecting hose 9 for counting, meanwhile, the pushing cylinder 17 is started to drive the rotating motor 16 to move downwards, so that the rotating motor 16 drives the linkage rotating rod 15 to move the washing liquid injection tube 11 downwards to the upper surface of the first positioning orifice plate 22, then the first driving motor 21 is started to drive the driving screw rod 19 to rotate forwards, the driving screw rod 19 drives the sleeving moving block 18 to move leftwards under the action of the screw thread, so that each injection head of the washing liquid injection tube 11 is injected into each hole site of the first positioning orifice plate 22 and each hole site in the second positioning orifice plate 29, each hole is 100 muL, one time of washing is performed at an interval of 6min, three times of washing, then, the vibration motor 34 is started to drive the decolorizing disc 33 to carry out vibration decolorizing, so that the second positioning hole plate 29 and the first positioning hole plate 22 generate vibration, and the decolorizing operation is completed.
Addition of the antigen to be evaluated: the rotary motor 16 is started to drive the linkage rotary rod 15 to rotate clockwise for ninety degrees, so that the injection tube 12 of the solution to be evaluated is positioned above the second positioning hole plate 29, the antigen solution in the antigen bearing box 5 to be evaluated can be injected into the injection tube 12 of the solution to be evaluated and can be injected into the hole of the second positioning hole plate 29 along the injection tube 12 of the solution to be evaluated, the first driving motor 21 is started to drive the driving screw 19 to rotate reversely, the driving screw 19 drives the sleeving moving block 18 to move rightwards under the action of the thread, 100 mu L of antigen can be added into each hole of the first positioning hole plate 22 and the second positioning hole plate 29, the temperature is set as 37 ℃, standing is carried out for 1 hour, and washing is carried out once again.
Adding a primary antibody: the rotating motor 16 can be started to drive the anti-dilution injection tube 13 to continue rotating, so that the anti-dilution injection tube 13 is located at the position of the upper surface of the first positioning hole plate 22, then anti-dilution liquid in the anti-dilution liquid containing box 6 enters the anti-dilution injection tube 13, the first driving motor 21 is started to drive the driving screw rod 19 to rotate forwards, the driving screw rod 19 drives the sleeve connection moving block 18 to move leftwards under the action of threads, 50 mu L of each hole is injected into the first positioning hole plate 22 and the second positioning hole plate 29, the incubator is incubated for 1h at 37 ℃, the primary antibody in each hole is discarded and dried, 100 mu L of each hole is added, 6 min/time and three times of washing are carried out.
Adding an enzyme-labeled secondary antibody: the rotary motor 16 can be started to drive the linkage rotating rod 15 to rotate ninety degrees continuously, namely, the secondary antibody diluent in the secondary antibody diluent containing box 7 can be injected into the secondary antibody diluent injection tube 14 and fall down along the secondary antibody diluent injection tube 14, the first driving motor 21 is started to drive the driving screw rod 19 to rotate reversely, the driving screw rod 19 drives the sleeve connection moving block 18 to move rightwards under the action of the screw thread, so that the anti-rabbit immunoglobulin antibody Abcam liquid marked by the secondary horseradish peroxidase is diluted according to the instruction, the liquid is sequentially moved and dripped in the holes of the second positioning hole plate 29 and the first positioning hole plate 22, a 50 mu L incubator is added into each hole for incubation at 37 ℃ for 1h, the rotary motor 16 is started again to be reset to the position of the washing liquid injection tube 11 aligned with the upper surface of the first positioning hole plate 22, the washing liquid is discarded and dried, 100 mu L of the washing liquid is added into each hole for 6 min/time, washing for five times, and finishing decoloring again;
when the waste water is collected and the washing liquid waste water is required to be collected, the washing liquid waste water can enter the washing liquid containing box 42 to complete the containing operation, when the antigen is to be evaluated, the servo motor 37 can be started to drive the rotating rod 36 to rotate ninety degrees, so that the cleaning solution storage box 42 drives the positioning fixture block 41 to rotate, the positioning fixture block 41 drives the linkage support block 39 to rotate outside the lantern ring shaft lever 40, and the washing liquid containing box 42 is always vertically downward under the action of gravity, so that the antigen recovery box 43 to be evaluated is butted at the lower position of the dilution box 1 to continuously collect the antigen to be evaluated, then the servo motor 37 can be started to drive the rotary rod 36 to continuously rotate, namely, the secondary antibody recovery tank 45 can be positioned at a position below the dilution tank 1, so that the secondary antibody dilution liquid can be collected, and the rotation is continued to make the primary anti-recovery tank 44 located at the position below the dilution tank 1 to carry out the loading operation of the primary anti-dilution liquid.
The points to be finally explained are: first, in the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounted," "connected," and "connected" should be understood broadly, and may be a mechanical connection or an electrical connection, or a communication between two elements, and may be a direct connection, and "upper," "lower," "left," and "right" are only used to indicate a relative positional relationship, and when the absolute position of the object to be described is changed, the relative positional relationship may be changed;
secondly, the method comprises the following steps: in the drawings of the disclosed embodiments of the invention, only the structures related to the disclosed embodiments are referred to, other structures can refer to common designs, and the same embodiment and different embodiments of the invention can be combined with each other without conflict;
and finally: the present invention is not limited to the above preferred embodiments, but rather, any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The utility model provides a seed of virus diluting device of pseudorabies virus vaccine research, includes dilutent case (1), its characterized in that: a protective door (2) is installed on the front side of the dilution box (1), one side of the protective door (2) is fixedly connected with a control button (3) on the position of the dilution box (1) through a bolt, and a dilution mechanism is arranged above the dilution box (1);
dilution mechanism holds vanning (4) including setting up the washing liquid in dilution case (1) top, the washing liquid holds vanning (4) one side and installs and waits to evaluate antigen and holds vanning (5), wait to evaluate antigen and hold vanning (5) one side and install an anti diluent and hold vanning (6) one side and be provided with two anti diluent and hold vanning (7) in an anti diluent, the washing liquid holds vanning (4), waits to evaluate antigen and holds vanning (5), an anti diluent and hold vanning (6), two anti diluent and hold vanning (7) top and all install pump machine (8), has coupling hose (9) in pump machine (8) output intercommunication, flowmeter (10) are installed to coupling hose (9) bottom, flowmeter (10) below is provided with the switching component.
2. The apparatus of claim 1, wherein the apparatus comprises: the switching component comprises a washing liquid injection pipe (11) arranged at the input end of the flow meter (10), one side of the washing liquid injection pipe (11) is provided with a liquid injection pipe (12) to be evaluated, an anti-dilution injection tube (13) is arranged at one side of the injection tube (12) of the liquid to be evaluated, and a secondary antibody dilution injection tube (14) is arranged on one side of the primary antibody dilution injection tube (13), four linkage rotating rods (15) are arranged in a gap formed among the washing liquid injection tube (11), the to-be-evaluated liquid injection tube (12), the primary anti-dilution injection tube (13) and the secondary anti-dilution injection tube (14), a rotating motor (16) is arranged at one end of the linkage rotating rod (15), a pressing cylinder (17) is arranged above the rotating motor (16), one side of the pressing cylinder (17) is connected with a sleeve connection moving block (18), and the inner wall of the sleeving moving block (18) is in threaded connection with a driving screw rod (19).
3. The device for diluting pseudorabies virus vaccine of claim 2, wherein: the external portion of the sleeve joint moving block (18) is provided with a sleeve joint frame (20), one end of the driving screw rod (19) is provided with a first driving motor (21), the first driving motor (21) is in coaxial transmission connection with the driving screw rod (19), and the inner wall of the dilution box (1) is provided with two heating resistance tubes (48).
4. The device for diluting pseudorabies virus vaccine of claim 2, wherein: washing liquid holds vanning (4), treats that the appraisal antigen holds vanning (5), an anti dilution holds vanning (6), two anti dilution hold vanning (7) and be annular equidistance and distribute the setting, be linked together between coupling hose (9) and flowmeter (10).
5. The apparatus of claim 1, wherein the apparatus comprises: the washing liquid injection tube (11) is provided with a linkage mechanism below, the linkage mechanism comprises a first positioning hole plate (22) arranged below the washing liquid injection tube (11), one side of the first positioning hole plate (22) is detachably connected with a sleeve barrel (23) through a bolt, a first driving rod (24) is arranged inside the sleeve barrel (23), one end of the first driving rod (24) is provided with a first transmission motor (25), the other end of the first driving rod (24) is provided with a second driving rod (26), one end of the second driving rod (26) is provided with a second transmission motor (28) which is connected in a coaxial transmission manner, a sleeve connecting block (27) is sleeved at a position between the sleeve barrel (23) and the second transmission motor (28) outside the second driving rod (26), and a second positioning hole plate (29) is connected at a position at one side of the sleeve connecting block (27), and a centrifugal frame (30) is sleeved outside the second driving rod (26) and positioned at the other side of the sleeving connection block (27).
6. The apparatus of claim 5, wherein the apparatus comprises: centrifugal branch (31) and install second driving motor (32) in centrifugal branch (31) below position department are installed in the embedding of centrifugal frame (30) inner wall bottom, second driving motor (32) outer wall connection has a color disc (33), color disc (33) one side is provided with two vibrating motor (34).
7. The apparatus of claim 5, wherein the apparatus comprises: the outer part of the first driving rod (24) is fixedly connected with the sleeve joint barrel (23), and the outer part of the second driving rod (26) is fixedly welded with the inner part of the sleeve joint block (27).
8. The apparatus of claim 5, wherein the apparatus comprises: the inner wall of the top end of the centrifugal frame (30) is movably connected with the outside of the second driving rod (26), and the outer wall of the sleeving block (27) is detachably connected with the second positioning hole plate (29) through a bolt.
9. The apparatus of claim 1, wherein the apparatus comprises: a supporting collar plate (35) is arranged at one side of the dilution box (1), a rotating rod (36) is embedded and arranged at one side of the supporting collar plate (35), one end of the rotating rod (36) is coaxially connected with a servo motor (37) in a transmission way, the other end of the rotating rod is provided with a rotating disk (38), four linkage supporting blocks (39) are distributed on one side of the rotating disc (38) in an annular shape at equal intervals, a lantern ring shaft lever (40) is arranged in the linkage support block (39), a positioning fixture block (41) is embedded and clamped at one side of the limiting spiral ring (46), a washing liquid storage box (42) is arranged at one side of the positioning fixture block (41), an antigen recovery box (43) to be evaluated is arranged below the cleaning solution containing box (42), an anti-recovery box (44) is arranged on one side of the antigen recovery box (43) to be evaluated, and a secondary antibody recovery tank (45) is installed at a position below the primary antibody recovery tank (44).
10. The apparatus of claim 9, wherein the apparatus comprises: one end of the rotating rod (36) penetrates through one side of the supporting ring plate (35) and extends to the other side of the supporting ring plate (35), the outer portion of the rotating rod (36) is movably connected with the inner portion of the supporting ring plate (35), the outer portion of the ring shaft rod (40) is movably connected with a coaxial bearing in the linkage supporting block (39), a limiting spiral ring (46) is connected to the outer portion of the ring shaft rod (40) and located on one side of the linkage supporting block (39) in a threaded mode, and a supporting block (47) is installed below the servo motor (37).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210137552.1A CN114471209A (en) | 2022-02-15 | 2022-02-15 | Seed of virus diluting device of pseudorabies virus bacterin research |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210137552.1A CN114471209A (en) | 2022-02-15 | 2022-02-15 | Seed of virus diluting device of pseudorabies virus bacterin research |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114471209A true CN114471209A (en) | 2022-05-13 |
Family
ID=81479566
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210137552.1A Pending CN114471209A (en) | 2022-02-15 | 2022-02-15 | Seed of virus diluting device of pseudorabies virus bacterin research |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114471209A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208711522U (en) * | 2018-06-14 | 2019-04-09 | 安吉艾格赛思生物科技有限公司 | A kind of biovaccine dilution device |
| CN213791284U (en) * | 2020-09-08 | 2021-07-27 | 舟山诚悦生物科技有限公司 | Biological bacterin diluting device |
| CN113289526A (en) * | 2021-04-22 | 2021-08-24 | 安徽福贸生物科技有限公司 | New coronavirus detect reagent box and use dilution equipment |
| CN214020626U (en) * | 2020-10-09 | 2021-08-24 | 常州同泰生物药业科技股份有限公司 | Seed of virus diluting device of pseudorabies virus bacterin research |
-
2022
- 2022-02-15 CN CN202210137552.1A patent/CN114471209A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208711522U (en) * | 2018-06-14 | 2019-04-09 | 安吉艾格赛思生物科技有限公司 | A kind of biovaccine dilution device |
| CN213791284U (en) * | 2020-09-08 | 2021-07-27 | 舟山诚悦生物科技有限公司 | Biological bacterin diluting device |
| CN214020626U (en) * | 2020-10-09 | 2021-08-24 | 常州同泰生物药业科技股份有限公司 | Seed of virus diluting device of pseudorabies virus bacterin research |
| CN113289526A (en) * | 2021-04-22 | 2021-08-24 | 安徽福贸生物科技有限公司 | New coronavirus detect reagent box and use dilution equipment |
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