CN114460325A - Liquid micro-flow control system and method and micro-flow control detection device - Google Patents
Liquid micro-flow control system and method and micro-flow control detection device Download PDFInfo
- Publication number
- CN114460325A CN114460325A CN202210070442.8A CN202210070442A CN114460325A CN 114460325 A CN114460325 A CN 114460325A CN 202210070442 A CN202210070442 A CN 202210070442A CN 114460325 A CN114460325 A CN 114460325A
- Authority
- CN
- China
- Prior art keywords
- liquid
- gas
- conveying pipeline
- liquid storage
- micro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D16/00—Control of fluid pressure
- G05D16/20—Control of fluid pressure characterised by the use of electric means
- G05D16/2006—Control of fluid pressure characterised by the use of electric means with direct action of electric energy on controlling means
- G05D16/2013—Control of fluid pressure characterised by the use of electric means with direct action of electric energy on controlling means using throttling means as controlling means
- G05D16/2026—Control of fluid pressure characterised by the use of electric means with direct action of electric energy on controlling means using throttling means as controlling means with a plurality of throttling means
- G05D16/204—Control of fluid pressure characterised by the use of electric means with direct action of electric energy on controlling means using throttling means as controlling means with a plurality of throttling means the plurality of throttling means being arranged in parallel
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D7/00—Control of flow
- G05D7/06—Control of flow characterised by the use of electric means
- G05D7/0617—Control of flow characterised by the use of electric means specially adapted for fluid materials
- G05D7/0629—Control of flow characterised by the use of electric means specially adapted for fluid materials characterised by the type of regulator means
- G05D7/0635—Control of flow characterised by the use of electric means specially adapted for fluid materials characterised by the type of regulator means by action on throttling means
- G05D7/0641—Control of flow characterised by the use of electric means specially adapted for fluid materials characterised by the type of regulator means by action on throttling means using a plurality of throttling means
- G05D7/0652—Control of flow characterised by the use of electric means specially adapted for fluid materials characterised by the type of regulator means by action on throttling means using a plurality of throttling means the plurality of throttling means being arranged in parallel
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
- G01N2035/1044—Using pneumatic means
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Fluid Mechanics (AREA)
- Automation & Control Theory (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Flow Control (AREA)
- Jet Pumps And Other Pumps (AREA)
Abstract
The invention relates to the technical field of biological fluid detection and analysis, and discloses a liquid micro-flow control system and method and a micro-flow control detection device, wherein the system comprises: an air compressor, an air pressure controller and a gas flow controller; the air compressor is communicated with a liquid storage for storing liquid to be treated through a first gas conveying pipeline, and the air compressor is sequentially communicated with the air pressure controller, the gas flow controller and the liquid storage in series through a second gas conveying pipeline; the air pressure controller is communicated with the liquid storage device through a third air conveying pipeline; control valves are arranged on the first gas conveying pipeline, the third gas conveying pipeline and a second gas conveying pipeline section positioned between the gas pressure controller and the gas flow controller; the air compressor, the air pressure controller, the gas flow controller and the control valve are respectively in signal connection with the control device. The invention realizes the accurate control of the liquid micro-flow in a non-contact mode.
Description
Technical Field
The invention relates to the technical field of biological fluid detection and analysis, in particular to a micro-liquid flow rate control system and method and a micro-fluidic detection device.
Background
Microfluidics (Microfluidics), which refers to the science and technology involved in systems using microchannels (tens to hundreds of microns in size) to process or manipulate tiny fluids (microliter in volume), is an emerging interdiscipline that involves chemical, fluid physics, microelectronics, new materials, biology, and biomedical engineering.
The microfluidic technology has received great attention in the field of biological fluid biopsy in recent years, and a high-precision microfluidic speed control technology is one of the difficulties. In addition, due to the characteristics of the body fluid biopsy, the detection system is required to perform rapid and accurate detection on the body fluid in a non-contact manner. Furthermore, the parts of the detection system that come into contact with body fluids are generally present as consumables, the cost requirement for a disposable use being higher.
At present, the micro-flow rate control is realized in a semi-automatic syringe pump mode in an industry laboratory through a manual setting mode, and the reuse of the syringe pump cannot be avoided. In addition, the mode of controlling the syringe to advance by the stepping motor at a certain speed has low precision, and the flow speed control is unstable in the process, so that the experimental detection effect is influenced. In addition, the mode that the motor pushes the syringe cannot sense the resistance change of the rear end, and the rear end is partially blocked and cannot be monitored. Therefore, the method is mainly used in a laboratory, a large amount of manual operation is required in the detection process, and the large-scale commercialization effect is not ideal.
Disclosure of Invention
Technical problem to be solved
The present invention aims to overcome the defects of the prior art and provide a system and a method for accurately controlling the micro-flow of liquid in a non-contact manner by constant-flow air pressure control, and a micro-fluidic detection device.
(II) technical scheme
In order to solve the above problems, the present invention provides a liquid micro-flow control system comprising: an air compressor, an air pressure controller and a gas flow controller; the air compressor is communicated with a liquid storage device used for storing liquid to be processed through a first gas conveying pipeline, and the air compressor is sequentially communicated with the air pressure controller, the gas flow controller and the liquid storage device in series through a second gas conveying pipeline; the air pressure controller is communicated with the liquid storage through a third air conveying pipeline; control valves are arranged on the first gas conveying pipeline, the third gas conveying pipeline and a second gas conveying pipeline section positioned between the gas pressure controller and the gas flow controller; the air compressor, the air pressure controller, the gas flow controller and the control valve are respectively in signal connection with a control device.
Optionally, the air pressure controller further comprises a pressure stabilizing container arranged on the second gas conveying pipeline and respectively communicated with the air compressor and the air pressure controller.
Optionally, the device further comprises an air pressure sensor communicated with the liquid storage, and used for detecting air pressure in the liquid storage, wherein the air pressure sensor is in signal connection with the control device.
Optionally, the device further comprises an alarm device, and the alarm device is in signal connection with the control device.
The invention also provides a liquid micro-flow control method, which uses the liquid micro-flow control system and comprises the following steps:
s1, closing control valves arranged on the second gas conveying pipeline and the third gas conveying pipeline through a control device;
s2, hermetically communicating a container containing liquid to be measured with a liquid storage device through a liquid conveying pipe and an input end of the liquid storage device; starting an air compressor through the control device, providing negative pressure to a liquid storage through a first gas conveying pipeline, and pumping liquid to be detected into the liquid storage; then stopping the air compressor;
s3, closing a control valve arranged on the first gas conveying pipeline through the control device, opening a control valve arranged on the third gas conveying pipeline, and starting the air compressor; adjusting the air pressure controller to convey air into the liquid storage to enable the air pressure in the liquid storage to reach a set value;
and S4, closing the control valve arranged on the third gas conveying pipeline through the control device, opening the control valve arranged on the second gas conveying pipeline, conveying gas into the liquid storage at a set flow rate through the gas flow controller, and discharging the liquid to be detected in the liquid storage at a set flow rate.
Optionally, in step S3, the air compressor is started to deliver air to the surge tank, and then the surge tank is used to deliver air to the air pressure controller.
Optionally, in step S4, when the gas pressure sensor detects that the gas pressure in the liquid storage exceeds the set threshold, the alarm device gives an alarm through an audible and visual signal.
The invention also provides a microfluidic detection device, which comprises the liquid microfluidic control system and a microfluidic detection package, wherein the microfluidic detection package comprises: the liquid storage device and the micro-fluidic chip are communicated with the outlet end of the liquid storage device.
(III) advantageous effects
According to the liquid micro-flow control system, the liquid micro-flow control method and the micro-flow control device, the air pressure controller and the gas flow controller can be used for accurately controlling the liquid micro-flow in a non-contact mode. The liquid level air pressure of the liquid to be detected in the liquid storage device can be quickly improved through the air pressure controller, the liquid level air pressure of the liquid to be detected in the liquid storage device can be finely adjusted through the air flow controller, the flow of the gas input into the liquid storage device can be accurately set according to needs, and therefore the micro-flow of the liquid to be detected in the micro-fluidic chip can be accurately controlled.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.
FIG. 1 is a schematic diagram of a liquid micro-flow control system according to an embodiment of the invention;
FIG. 2 is a process flow diagram of a liquid micro-flow control method according to an embodiment of the invention.
The reference numbers in the drawings are, in order:
1. the device comprises an air compressor, 2, an air pressure controller, 3, an air flow controller, 4, a liquid storage device, 5, a pressure stabilizing container, 6, an air pressure sensor, 7, a micro-fluidic chip, 10, a first air conveying pipeline, 20, a second air conveying pipeline, 30, a third air conveying pipeline, 40 and a control valve.
Detailed Description
The following describes the embodiments of the present invention in further detail with reference to the examples and the accompanying drawings. The following examples of the present invention are provided herein to illustrate the present invention, but are not intended to limit the scope of the present invention.
As shown in fig. 1, an embodiment of the present invention provides a liquid micro-flow control system, including: an air compressor 1, an air pressure controller 2 and a gas flow controller 3.
The air compressor 1 communicates with a liquid reservoir 4 for storing a liquid to be treated via a first gas delivery line 10; and a control valve 40 is provided in the first gas delivery conduit 10 for controlling the gas transport in the first gas delivery conduit 10. The air compressor 1 is sequentially communicated with the air pressure controller 2, the gas flow controller 3 and the liquid storage 4 in series through a second gas conveying pipeline 20; and a control valve 40 is provided on a section of the second gas delivery pipe 20 between the gas pressure controller 2 and the gas flow controller 3 for controlling the gas transfer between the gas pressure controller 2 and the gas flow controller 3 in the second gas delivery pipe 20. The air pressure controller 2 is also communicated with the liquid storage 4 through a third air conveying pipeline 30; so that there are two parallel gas delivery channels in the gas pressure controller 2 and the liquid reservoir 4. A control valve 40 is also provided on the third gas delivery duct 30 for controlling the gas transport in the third gas delivery duct 30.
The air compressor 1, the air pressure controller 2, the gas flow controller 3 and the control valves 40 arranged on the first, second and third gas delivery pipelines are respectively in signal connection with a control device (not shown in the figure), so that the control device can control the starting, running and stopping of the air compressor 1, the air pressure controller 2 and the gas flow controller 3 and the opening and closing of the control valves 40. In the embodiment, the air compressor 1 can provide positive pressure gas for the system and can also form negative pressure in the pipeline in the system; the air pressure controller 2 may be a precise air pressure regulating valve, and obtains a certain air pressure by regulating air quantity. The gas flow controller 3 may be a constant flow gas flow controller capable of continuous, high precision, micro gas flow control, for example, 10 ± 0.01 ml/min. The control valve 40 may be a solenoid valve. The control device can be realized by a single chip microcomputer and can achieve the control speed of millisecond level.
In one embodiment, a surge tank 5 is disposed between the air compressor 1 and the air pressure controller 2, and the surge tank 5 is communicated with the air compressor 1 and the air pressure controller 2 through a second gas delivery pipe 20, respectively. The surge tank 5 may be used to store the gas delivered from the air compressor 1.
In one embodiment, an air pressure sensor 6 is provided in communication with the liquid reservoir 4 for sensing air pressure within the liquid reservoir 4. The air pressure sensor 6 is in signal connection with the control device, so that the control device can read air pressure data in the liquid reservoir 4 detected by the air pressure sensor 6.
In one embodiment, an alarm device is provided in signal connection with the control device, and when the air pressure value in the liquid storage 4 read by the control device exceeds a set threshold value, the control device enables the alarm device to give an alarm through an audible and visual signal.
As shown in fig. 2, the embodiment of the invention further provides a liquid micro-flow control method, which uses the liquid micro-flow control system, and comprises the following steps:
s1, the control valves 40 provided on the second gas delivery pipe 20 and the third gas delivery pipe 30 are closed by the control means.
Before step S1, the ends of the first gas delivery pipe 10, the second gas delivery pipe 20 and the third gas delivery pipe 30 are respectively connected to the liquid storage 4 through the input end of the liquid storage 4 in a sealing manner, and a setting device, such as a quick gas pipe joint, can be used to make the ends of the first gas delivery pipe 10, the second gas delivery pipe 20 and the third gas delivery pipe 30 be conveniently and quickly connected to the input end of the liquid storage 4 in a sealing manner.
S2, hermetically communicating the container containing the liquid to be detected with the liquid storage 4 through the liquid conveying pipe and the input end of the liquid storage 4; starting the air compressor 1 through a control device, providing negative pressure to the liquid storage 4 through the first gas conveying pipeline 10, and pumping liquid to be measured into the liquid storage 4; the air compressor 1 is then stopped.
The container containing the liquid to be tested may be a test tube containing the blood sample to be tested. The liquid supply line can also be connected in a sealing manner to the inlet section of the liquid reservoir 4 by means of a quick-action gas line connection. The air compressor pump 1 is started to discharge the gas in the liquid reservoir 4 through the first gas delivery pipe 10, and a negative pressure is formed in the liquid reservoir 4. The liquid to be measured contained in the test tube is then drawn into the liquid reservoir 4 by means of the pressure difference. After that, the air compressor 1 is stopped by the control device.
S3, closing the control valve 40 arranged on the first gas conveying pipeline 10 and opening the control valve 40 arranged on the third gas conveying pipeline 30 through the control device; starting the air compressor 1; the air pressure controller 2 is adjusted to feed air into the liquid storage 4 so that the air pressure in the liquid storage 4 reaches a set value.
In step S3, the air compressor (1) is started to supply air to the surge tank (5), and then the surge tank (5) supplies air to the air pressure controller (2).
The gas communication between the air compressor 1 and the liquid reservoir 4 can be stopped by the control device closing a control valve 40 arranged on the first gas delivery line 10. The air compressor 1 is started by the control device to deliver gas to the pressure stabilizing container 5, when the gas pressure stored in the pressure stabilizing container 5 reaches a desired value, the air compressor 1 can be closed by the control device, and then the flow of the liquid to be measured in the liquid storage 4 is controlled by only utilizing the gas in the pressure stabilizing container 5. This can reduce the operating time of the air compressor 1.
The control valve 40 provided on the third gas delivery pipe 30 is opened by the control device, and the gas pressure controller 2 is adjusted so that the gas in the surge tank 5 is introduced into the liquid reservoir 4 through the gas pressure controller 2 and the third gas delivery pipe 30. The air pressure controller 2 can adjust the air pressure in the liquid storage 4 by adjusting the amount of air supplied to the liquid storage 4 by the air pressure value fed back from the liquid storage 4 until a set value is reached.
S4, the control device closes the control valve 40 provided on the third gas delivery pipe 30, opens the control valve 40 provided on the second gas delivery pipe 20, delivers the gas into the liquid storage 4 at a set flow rate through the gas flow controller 3, and discharges the liquid to be detected in the liquid storage 4 at a set flow rate.
The control valve 40 provided on the third gas delivery pipe 30 is closed so that the gas supply from the gas pressure controller 2 directly to the liquid storage 4 is stopped. The control valve 40 provided on the second gas delivery pipe 20 is opened so that the gas output from the surge tank 5 is input into the liquid reservoir 4 through the gas pressure controller 2 and the gas flow controller 3.
When the outlet end of the liquid reservoir 4 is connected to a detection device for performing relevant detection, for example, a microfluidic chip, the liquid to be detected in the liquid reservoir 4 flows into the microfluidic chip under pressure. Because the resistance inside the microfluidic chip is constant, the flow rate of the liquid to be detected in the microfluidic chip can be controlled by controlling the liquid level and the air pressure of the liquid to be detected in the liquid storage 4. In the closed space formed between the liquid level of the liquid to be measured and the output port of the gas flow controller 3, the liquid level air pressure of the liquid to be measured in the liquid storage 4 can be finely adjusted through the gas flow controller 3, and the liquid level air pressure can reach an equilibrium state, namely the liquid level air pressure is kept unchanged. In this state, the volume of gas entering the liquid reservoir 4 is equal to the volume of liquid exiting the liquid reservoir 4. Gas is delivered into the liquid storage 4 at a set flow rate through the gas flow controller 3, so that the liquid to be detected in the liquid storage 4 flows into the microfluidic chip at a set flow rate.
The control device can calculate the flow rate of the liquid to be measured according to the liquid surface air pressure in the liquid storage 4 detected by the air pressure sensor 6 and the flow rate of the gas output by the gas flow controller 3, and can also control the liquid surface air pressure of the liquid to be measured in a balanced state to control the flow rate of the liquid to be measured by the flow rate of the gas output by the gas flow controller 3.
The control device sets the gas flow input into the liquid storage 4 by the gas flow controller 3 and measures the unit time variation of the liquid level air pressure in the liquid storage 4, and according to an ideal gas state equation, PV is nRT, wherein P is the liquid level air pressure of the liquid to be measured in the liquid storage 4; v is the volume of gas flowing into the liquid reservoir 4 per unit time; n is the number of moles of gas, R is the molar gas constant, and T is the thermodynamic temperature. Under the condition that the compression coefficient Z is constant, the liquid level and the air pressure of the liquid to be measured in the liquid storage 4 and the flow rate of the gas input by the gas flow controller 3, which are required for enabling the liquid to be measured entering the microfluidic chip to reach the set flow rate, can be calculated according to the formula. The control device can also rapidly adjust the level air pressure of the liquid to be measured in the liquid storage 4 according to the detected flow rate so as to adjust the flow rate to the set flow rate.
Assume that the gas-per-second equivalent volume currently flowing through gas flow controller 3 into the enclosed area in liquid reservoir 4 is VQi (Qi)The parameter may be set by the control device; the current gas volume in the liquid reservoir 4 is V and the gas pressure P, when the gas pressure difference is very small, assuming that the compression factors Z are equal, then
PVQi (Qi)＝ΔnRTZ
Wherein Δ n is the number of moles of gas added;
P(V+Vqi (Qi))＝(n+Δn)RTZ
Where n is the number of moles of gas in the enclosed area in the liquid reservoir 4 before the gas is added;
the closed area in the liquid reservoir 4 discharges a volume V of liquidLiquid for treating urinary tract infectionAfter the lapse of this second, the air pressure in the closed area in the liquid reservoir 4 becomes P2，
P2(V+VLiquid for treating urinary tract infection)＝(n+Δn)RTZ
P2(V+VLiquid for treating urinary tract infection)＝P(V+VQi (Qi))
VLiquid for treating urinary tract infection＝P(V+VQi (Qi))/P2-V
When the closed area in the liquid reservoir 4 is in equilibrium, P2P, from which it follows that,
Vliquid for treating urinary tract infection＝VQi (Qi)
In summary, the flow rate of the liquid to be measured in the microfluidic chip can be controlled by controlling the liquid level air pressure of the liquid to be measured in the liquid storage 4, and the liquid level air pressure and the liquid flow rate are positively correlated. The flow rate value can be observed in real time through the derivation of an ideal gas equation, and the reaction speed of flow rate control depends on the liquid level and air pressure regulation speed. After the final speed is reached, the constant pressure is kept, namely the required control flow rate is reached. When bubbles, dust or other particles are blocked in the microfluidic chip to cause resistance to increase, so that the flow speed of the liquid to be detected in the microfluidic chip is reduced, and the liquid level air pressure of the liquid to be detected in the liquid storage 4 is increased; the air pressure sensor 6 sends the detected pressure value to the control device. The control device will increase the flow of the transport gas through the gas flow controller 3, thereby increasing the pressure to restore the flow rate of the liquid to be measured to the set flow rate. If the liquid level pressure of the liquid to be measured in the liquid storage 4 continuously increases and exceeds a set threshold value, the control device controls an alarm device (not shown in the figure) to alarm through an audible and visual signal. At the same time, the gas flow controller 3 is controlled to stop the gas supply into the liquid reservoir 4.
As shown in fig. 1, the embodiment of the present invention further provides a microfluidic detection device, which includes the above-mentioned liquid micro-flow control system and the microfluidic detection package. The microfluidic detection package includes: a liquid storage 4 and a micro-fluidic chip 7 communicated with the outlet end of the liquid storage 4. In the detection process, the microfluidic detection package can be an independent consumable package, and by means of quick connection and separation with the liquid micro-flow control system, non-contact is realized in the detection process, the liquid micro-flow is accurately controlled, and the independent consumable package can be produced in large batch, so that the detection cost is reduced.
According to the liquid micro-flow control system, the liquid micro-flow control method and the micro-flow control device, the air pressure controller and the gas flow controller can be used for accurately controlling the liquid micro-flow in a non-contact mode. The liquid level air pressure of the liquid to be detected in the liquid storage device can be quickly improved through the air pressure controller, the liquid level air pressure of the liquid to be detected in the liquid storage device can be finely adjusted through the air flow controller, the flow of the gas input into the liquid storage device can be accurately set according to needs, and therefore the micro-flow of the liquid to be detected in the micro-fluidic chip can be accurately controlled. Moreover, the system has small volume and low power consumption. The microfluidic detection package can be used as an independent consumable package for mass production, and the detection cost is reduced.
In the present invention, terms such as "upper", "lower", "left", "right", "front", "rear", "vertical", "horizontal", "side", "bottom", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only terms of relationships determined for convenience of describing structural relationships of the parts or elements of the present invention, and are not intended to refer to any parts or elements of the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; the connection can be mechanical connection or signal connection; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. To those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in specific cases and should not be construed as limiting the present invention.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
The above embodiments are merely illustrative of the present invention and are not to be construed as limiting the invention. Although the present invention has been described in detail with reference to the embodiments, it should be understood by those skilled in the art that various combinations, modifications or equivalents may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention, and the technical solution of the present invention is covered by the claims of the present invention.
Claims (8)
1. A system for micro-fluidic control of a liquid, comprising: the air compressor (1), the air pressure controller (2) and the gas flow controller (3);
the air compressor (1) is communicated with a liquid storage (4) for storing liquid to be treated through a first gas conveying pipeline (10);
the air compressor (1) is sequentially communicated with the air pressure controller (2), the gas flow controller (3) and the liquid storage (4) in series through a second gas conveying pipeline (20);
the air pressure controller (2) is communicated with the liquid storage (4) through a third air conveying pipeline (30);
control valves (40) are arranged on the first gas conveying pipeline (10), the third gas conveying pipeline (30) and the section of the second gas conveying pipeline (20) between the gas pressure controller (2) and the gas flow controller (3);
the air compressor (1), the air pressure controller (2), the gas flow controller (3) and the control valve (40) are respectively in signal connection with a control device.
2. The liquid micro-flow control system as claimed in claim 1, further comprising a surge tank (5) provided on the second gas delivery pipe (20) in communication with the air compressor (1) and the air pressure controller (2), respectively.
3. The liquid micro-flow control system as claimed in claim 2, further comprising an air pressure sensor (6) in communication with the liquid reservoir (4) for detecting air pressure within the liquid reservoir (4), the air pressure sensor (6) being in signal connection with the control means.
4. The liquid micro-flow control system of claim 3, further comprising an alarm device in signal communication with the control device.
5. A liquid micro-flow control method using the liquid micro-flow rate control system according to any one of claims 1 to 4, comprising the steps of:
s1, closing the control valves (40) arranged on the second gas conveying pipeline (20) and the third gas conveying pipeline (30) through a control device;
s2, hermetically communicating a container containing liquid to be measured with a liquid storage (4) through a liquid conveying pipe and an input end of the liquid storage (4); starting an air compressor (1) through the control device, providing negative pressure to the liquid storage (4) through a first gas conveying pipeline (10), and pumping liquid to be measured into the liquid storage (4); then stopping the operation of the air compressor (1);
s3, closing a control valve (40) arranged on the first gas conveying pipeline (10) through the control device, opening the control valve (40) arranged on the third gas conveying pipeline (30), and starting the air compressor (1); adjusting a gas pressure controller (2) to convey gas into the liquid storage (4) to enable the gas pressure in the liquid storage (4) to reach a set value;
s4, closing the control valve (40) arranged on the third gas conveying pipeline (30) through the control device, opening the control valve (40) arranged on the second gas conveying pipeline (20), conveying gas into the liquid storage device (4) at a set flow rate through the gas flow controller (3), and discharging the liquid to be detected in the liquid storage device (4) at a set flow rate.
6. The method for controlling the flow rate of a liquid according to claim 5, wherein in step S3, the air compressor (1) is started to supply gas to the surge tank (5), and then the surge tank (5) is used to supply gas to the pneumatic controller (2).
7. The liquid micro-flow control method according to claim 5, characterized in that in step S4, when the gas pressure sensor (6) detects that the gas pressure in the liquid reservoir (4) exceeds a set threshold value, the alarm means gives an alarm by an audible and visual signal.
8. A microfluidic detection device comprising the liquid micro-flow control system according to any one of claims 1 to 4 and a microfluidic detection pack,
the microfluidic detection package includes: the device comprises a liquid storage (4) and a micro-fluidic chip (7) communicated with the outlet end of the liquid storage (4).
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210070442.8A CN114460325A (en) | 2022-01-21 | 2022-01-21 | Liquid micro-flow control system and method and micro-flow control detection device |
| PCT/CN2022/123310 WO2023138102A1 (en) | 2022-01-21 | 2022-09-30 | Liquid micro-flow control system and method, and microfluidic test device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210070442.8A CN114460325A (en) | 2022-01-21 | 2022-01-21 | Liquid micro-flow control system and method and micro-flow control detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114460325A true CN114460325A (en) | 2022-05-10 |
Family
ID=81408905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210070442.8A Pending CN114460325A (en) | 2022-01-21 | 2022-01-21 | Liquid micro-flow control system and method and micro-flow control detection device |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN114460325A (en) |
| WO (1) | WO2023138102A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023138102A1 (en) * | 2022-01-21 | 2023-07-27 | 苏州莱博睿思生物科技有限公司 | Liquid micro-flow control system and method, and microfluidic test device |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9557744B2 (en) * | 2012-01-20 | 2017-01-31 | Mks Instruments, Inc. | System for and method of monitoring flow through mass flow controllers in real time |
| CN104696706B (en) * | 2015-01-20 | 2017-08-04 | 哈尔滨工业大学 | The microfluid stream quantity regulating device driven based on air pressure |
| JP6931540B2 (en) * | 2017-02-27 | 2021-09-08 | シスメックス株式会社 | Liquid feeding method using a sample processing chip, liquid feeding device for a sample processing chip |
| CN108679448B (en) * | 2018-06-02 | 2020-05-05 | 哈尔滨工业大学 | Microfluid flow online adjusting device and detection method |
| CN114460325A (en) * | 2022-01-21 | 2022-05-10 | 苏州莱博睿思生物科技有限公司 | Liquid micro-flow control system and method and micro-flow control detection device |
-
2022
- 2022-01-21 CN CN202210070442.8A patent/CN114460325A/en active Pending
- 2022-09-30 WO PCT/CN2022/123310 patent/WO2023138102A1/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023138102A1 (en) * | 2022-01-21 | 2023-07-27 | 苏州莱博睿思生物科技有限公司 | Liquid micro-flow control system and method, and microfluidic test device |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023138102A1 (en) | 2023-07-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10639662B2 (en) | Apparatus and method for dispensing or aspirating fluid | |
| US9459128B2 (en) | Device and method for dispensing or receiving a liquid volume | |
| JP4877710B2 (en) | Liquid processing apparatus and liquid supply method | |
| WO2006045229A1 (en) | A micro-volume liquid ejection system | |
| CN104696706A (en) | Micro fluid flow adjusting device based on air pressure drive | |
| CN108679448B (en) | Microfluid flow online adjusting device and detection method | |
| US20020158079A1 (en) | Liquid dispensing system with enhanced filter | |
| JP2005527814A (en) | Chemical mixture and transport system and method | |
| JP2008521087A (en) | Liquid supply system | |
| KR20170069254A (en) | Low pressure fluctuation flow control apparatus and method | |
| WO2016095685A1 (en) | Pneumatic constant pressure continuous liquid feeding device and method | |
| EP2830744B1 (en) | Micro flow filtration system and flow filtration method | |
| WO2003066509A2 (en) | Liquids dispensing systems and methods | |
| CN114460325A (en) | Liquid micro-flow control system and method and micro-flow control detection device | |
| KR101559161B1 (en) | Flow control device for removal of bubbles and pulsatility of sample flow into microfludic chips, flow control method, and flow control microfludic system | |
| EP1600746A2 (en) | Fluid dispenser cartridge with bladder means | |
| CN110709161A (en) | Synthesizer and metering mechanism | |
| CN111433584B (en) | Diluent preparation module and unit | |
| CN114127419B (en) | Control device and method for controlling a sensorless diaphragm pump | |
| CN107511188B (en) | Pneumatic control micro valve device based on chip laboratory microfluid technology and control method thereof | |
| US20220323982A1 (en) | Dosing device and method of dosing liquid media | |
| JP3905070B2 (en) | Pressure supply device | |
| CN113058496B (en) | Solution preparation method and solution preparation device | |
| CN206919968U (en) | A kind of air bubble type liquid level gauge supplies realization device | |
| US20240209847A1 (en) | Control arrangement and method for controlling a sensorless membrane pump |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |