CN114456906A - Novel nucleic acid sampling and storing tube - Google Patents
Novel nucleic acid sampling and storing tube Download PDFInfo
- Publication number
- CN114456906A CN114456906A CN202210273991.5A CN202210273991A CN114456906A CN 114456906 A CN114456906 A CN 114456906A CN 202210273991 A CN202210273991 A CN 202210273991A CN 114456906 A CN114456906 A CN 114456906A
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- China
- Prior art keywords
- dripper
- nucleic acid
- cap
- sampling
- screw cap
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 45
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 45
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 45
- 238000005070 sampling Methods 0.000 title claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 239000004033 plastic Substances 0.000 claims abstract description 13
- 229920003023 plastic Polymers 0.000 claims abstract description 13
- 239000003761 preservation solution Substances 0.000 claims abstract description 11
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000004321 preservation Methods 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 15
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 6
- 241000700605 Viruses Species 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 206010064091 Iatrogenic infection Diseases 0.000 description 3
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 3
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 3
- 210000003128 head Anatomy 0.000 description 3
- 210000001331 nose Anatomy 0.000 description 3
- 210000003800 pharynx Anatomy 0.000 description 3
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 description 2
- 108010066057 cabin-1 Proteins 0.000 description 2
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 210000003928 nasal cavity Anatomy 0.000 description 2
- 210000003300 oropharynx Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000711573 Coronaviridae Species 0.000 description 1
- 241000712431 Influenza A virus Species 0.000 description 1
- 241000713196 Influenza B virus Species 0.000 description 1
- 208000002979 Influenza in Birds Diseases 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000005224 forefinger Anatomy 0.000 description 1
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 231100000206 health hazard Toxicity 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 208000037797 influenza A Diseases 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 210000003813 thumb Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
- B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
- B01L3/50825—Closing or opening means, corks, bungs
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Clinical Laboratory Science (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a novel nucleic acid sampling and storing tube which comprises a plastic container device and nucleic acid storing liquid, wherein the plastic container device comprises a tube bin, a dripper screw cap, a dripper plug, a dripper cap and a sampling screw cap, the tube bin is of an oblong cavity structure, an opening at one end is connected with the dripper screw cap through threads, an opening at the other end is connected with the sampling screw cap through threads, the dripper screw cap is of a round cap-shaped structure, threads are arranged in the opening at one end and connected with the tube bin, and an opening at one end is connected with the dripper cap. The nucleic acid preservation solution is a guanidine-free liquid and comprises sodium dodecyl sulfate, water and other liquids. The principle of the invention is that a physical dripper device is arranged on the device, and a sample is directly added into the detection pre-device through the dripper without using a sample injector and a suction head. Simple to use, small in size, light in weight, low in price and popular with hospitals and medical examination laboratories.
Description
Technical Field
The invention belongs to the field of medical instruments, and mainly relates to a novel nucleic acid sampling and storing tube for detecting various nucleic acids.
Background
At present, the sampling of viruses such as new coronavirus, influenza A virus, influenza B virus, hand-foot-and-mouth virus, common influenza, highly pathogenic avian influenza, influenza A H1N1 and the like is generally carried out by collecting a sample from the oropharynx or the nasal cavity by using a pharyngeal or nasal swab, storing the sample in a test tube with a nucleic acid storage solution, and then sending the sample to a laboratory for nucleic acid detection. Other biological nucleic acid samples are also put into a test tube with nucleic acid preservation solution for preservation after being collected, and then sent to a laboratory for nucleic acid detection. The test tube with the nucleic acid preservation solution is generally called a virus sampling preservation tube or a nucleic acid sampling preservation tube, and is a commercialized product. However, there are some problems to be solved in the current commercial virus sampling and storing tube or nucleic acid sampling and storing tube, such as: the sample is put into the nucleic acid sampling and storing tube and then is mixed uniformly, when the nucleic acid detection is carried out, an operator needs to open a tube cover of the nucleic acid sampling and storing tube, a sample injector is used for installing a suction head, and liquid is sucked out of the nucleic acid sampling and storing tube for nucleic acid detection. Currently, most of preservation solutions in nucleic acid sampling and preserving tubes in domestic markets contain guanidine substances (guanidine isothiocyanate, guanidine thiocyanate, guanidine rhodamine or the like). since the reaction between guanidine thiocyanate or similar chemicals and bleaching agent (sodium hypochlorite) generates a byproduct, namely cyanide gas, and the cyanide gas can bring health hazards to laboratory workers, the U.S. Food and Drug Administration (FDA) warns the laboratory workers not to use or receive such samples.
Disclosure of Invention
The invention aims to provide a novel nucleic acid sampling and storing tube of non-toxic and guanidine component-free nucleic acid storing solution, which can directly add a sample into a detection pre-device through a dripper without using a sample injector and a sucker. Reducing working procedures, preventing the risk of sample pollution and preventing the risk of iatrogenic infection.
Therefore, in order to achieve the purpose, the invention provides a novel nucleic acid sampling and storing tube, which comprises a plastic container device and a nucleic acid storing liquid, wherein the plastic container device comprises a tube bin, a dripper screw cap, a dripper plug, a dripper cap and a sampling screw cap, the tube bin is of an oblong cavity structure, one end opening is connected with the dripper screw cap through threads, the other end opening is connected with the sampling screw cap through threads, the dripper screw cap is of a circular cap structure, the opening at one end is internally provided with threads and is connected with the tube bin, the opening at one end is connected with the dripper cap, the dripper is of a cross shape, one linear center is of a cavity shape, the opening at one end is connected with the tube bin through splicing, the opening at the other end is connected with the dripper plug through splicing, the dripper plug is of a small oblong solid plug, one end is connected with the inner side of the dripper cap, the other end is inserted into the dripper, the drip cover be circular lid column structure, one end is sealed, the other end is connected with the dripper spiral cover, the sampling spiral cover be circular lid column structure, one end is sealed, the other end is connected with the pipe storehouse. The nucleic acid preservation solution is a guanidine-free liquid and comprises sodium dodecyl sulfate, water and other liquids. The tube bin, the dripper screw cap, the dripper plug, the dripper cap and the sampling screw cap are made of medical plastics. The volume of the liquid bin is 10-30ml, and 5-20ml of nucleic acid preservation liquid is pre-filled in the liquid bin.
The principle of the invention is that a physical dripper device is arranged on the device, and a sample is directly added into the detection pre-device through the dripper without using a sample injector and a suction head. Reducing working procedures, preventing the risk of sample pollution and preventing the risk of iatrogenic infection.
In conclusion, the invention has the advantages that: simple to use, small in size, light in weight, low in price and popular with hospitals and medical examination laboratories.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification.
FIG. 1 is a schematic structural diagram of the present invention.
In the drawings: 1. a pipe bin; 2. the dripper is screwed with a cover; 3. a dripper; 4. blocking the drip opening; 5. a dropping cover; 6. a nucleic acid preservation solution; 7. and (4) sampling and screwing a cover.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Referring to the figure, the invention provides a novel nucleic acid sampling and storing tube, which comprises a plastic container device and a nucleic acid storing liquid, wherein the plastic container device comprises a tube bin 1, a dripper screw cap 2, a dripper 3, a dripper plug 4, a dripper cap 5 and a sampling screw cap 7, the tube bin 1 is in a long round cavity structure, one end opening is connected with the dripper screw cap 2 through screw threads, the other end opening is connected with the sampling screw cap 7 through screw threads, the dripper screw cap 2 is in a round cap structure, screw threads are arranged in the end opening and connected with the tube bin 1, one end opening is connected with the dripper cap 5, the dripper 3 is in a cross shape, one straight-line-shaped center is in a cavity shape, one end opening is connected with the tube bin 1 through insertion connection, the other end opening is connected with the dripper plug 4 through insertion connection, the dripper plug 4 is in a small long round solid plug shape, and one end is connected with the inner side of the dripper cap 5, the other end inserts in the dripper, the dripper 5 be circular lid column structure, one end is sealed, the other end is connected with dripper spiral cover 2, sampling spiral cover 7 be circular lid column structure, one end is sealed, the other end is connected with pipe storehouse 1 mutually. The nucleic acid preservation solution 6 is a guanidine-free solution, and comprises sodium dodecyl sulfate, water and other liquids. The tube bin 1, the dripper spiral cover 2, the dripper 3, the drip plug 4, the drip cover 5 and the sampling spiral cover 7 are made of medical plastics. The volume of the liquid cabin 1 is 10-30ml, and 5-20ml of nucleic acid preservation liquid 6 is pre-filled in the liquid cabin 1.
When the invention is used, firstly, an operator takes the plastic container device filled with the nucleic acid preservation solution 6 out of the packaging box, the sampling screw cap 7 on the tube bin 1 is upward and placed on the detection bracket, please the person to be detected sits beside the detection table, the person to be detected firstly rotates the sampling screw cap 7 on the tube bin 1 off from the tube bin 1, then collects a sample from the oropharynx part or the nasal cavity part of the person to be detected according to the operation rule by using a pharynx or a nose swab, then inserts the flocking part of the pharynx or the nose swab into the nucleic acid preservation solution 6 in the tube bin 1 and breaks off from the breakage point of the pharynx or the nose swab, then rotatably installs the sampling screw cap 7 on the tube bin 1, at the moment, the plastic container device is turned upside down, the sampling screw cap 7 on the tube bin 1 is inserted into the detection bracket to play a certain role of uniform mixing, and sends the bracket filled with the sample to a nucleic acid detection room, and rotating the dripper screw cap 2 on the pipe bin 1, separating the drip plug 4 on the dripper cap 5 from the dripper 3, holding the pipe bin 1 with one hand of an operator, aligning the dripper 3 in the detection pre-device, simultaneously squeezing the pipe bin 1 with a forefinger and a thumb, adding the nucleic acid preservation solution 6 and the nucleic acid sample in the pipe bin 1 into the detection pre-device, and preparing for detection on a computer. The dripper screw cap 2 is then rotatably mounted on the cartridge 1 and discarded in a waste bin.
The principle of the invention is that a physical dripper device is arranged on the device, and a sample is directly added into the detection pre-device through the dripper without using a sample injector and a suction head. Reducing working procedures, preventing the risk of sample pollution and preventing the risk of iatrogenic infection. The invention has the advantages that: simple to use, small in size, light in weight, low in price and popular with hospitals and medical examination laboratories.
The above detailed description is provided for a novel nucleic acid sampling and storing tube provided by the present application, and the principle and the embodiment of the present application are explained by applying specific examples, and the description of the above examples is only used to help understanding the method and the core concept of the present application; meanwhile, for a person skilled in the art, according to the idea of the present application, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present application.
Claims (4)
1. A novel nucleic acid sampling and preserving tube is characterized in that: comprises a plastic container device and nucleic acid preserving fluid, the plastic container device comprises a pipe bin, a dripper screw cap, a dripper plug, a dripper cap and a sampling screw cap, the pipe bin is in a long circle cavity structure, one end opening is connected with the dripper screw cap through screw threads, the other end opening is connected with the sampling screw cap through screw threads, the dripper screw cap is in a round cap structure, one end opening is internally provided with screw threads and is connected with the pipe bin, one end opening is connected with the dripper cap, the dripper is in a cross shape, one straight center is in a cavity shape, one end opening is connected with the pipe bin through splicing, the other end opening is connected with the dripper plug through splicing, the dripper plug is in a small circle solid plug shape, one end is connected with the inner side of the dripper cap, the other end is inserted into the dripper, the dripper cap is in a round cap structure, one end is sealed, the other end is connected with the dripper screw cap, the sampling spiral cover is in a circular cover-shaped structure, one end of the sampling spiral cover is sealed, and the other end of the sampling spiral cover is connected with the pipe bin.
2. The novel nucleic acid sampling and storing tube according to claim 1, wherein: the nucleic acid preservation solution is a guanidine-free liquid and comprises sodium dodecyl sulfate, water and other liquids.
3. The novel nucleic acid sampling and storing tube according to claim 1, wherein: the tube bin, the dripper screw cap, the dripper plug, the dripper cap and the sampling screw cap are made of medical plastics.
4. The novel nucleic acid sampling and storing tube according to claim 1, wherein: the volume of the liquid bin is 10-30ml, and 5-20ml of nucleic acid preservation liquid is pre-filled in the liquid bin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210273991.5A CN114456906A (en) | 2022-03-20 | 2022-03-20 | Novel nucleic acid sampling and storing tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210273991.5A CN114456906A (en) | 2022-03-20 | 2022-03-20 | Novel nucleic acid sampling and storing tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114456906A true CN114456906A (en) | 2022-05-10 |
Family
ID=81416635
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210273991.5A Pending CN114456906A (en) | 2022-03-20 | 2022-03-20 | Novel nucleic acid sampling and storing tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114456906A (en) |
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2022
- 2022-03-20 CN CN202210273991.5A patent/CN114456906A/en active Pending
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