CN114451511A - Antibacterial nano-particles and preparation method and application thereof - Google Patents

Antibacterial nano-particles and preparation method and application thereof Download PDF

Info

Publication number
CN114451511A
CN114451511A CN202210008131.9A CN202210008131A CN114451511A CN 114451511 A CN114451511 A CN 114451511A CN 202210008131 A CN202210008131 A CN 202210008131A CN 114451511 A CN114451511 A CN 114451511A
Authority
CN
China
Prior art keywords
antibacterial
polyphenol
zein
nanoparticles
particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202210008131.9A
Other languages
Chinese (zh)
Other versions
CN114451511B (en
Inventor
尹寿伟
曹晓璇
黄小楠
杨晓泉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
South China University of Technology SCUT
Original Assignee
South China University of Technology SCUT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by South China University of Technology SCUT filed Critical South China University of Technology SCUT
Priority to CN202210008131.9A priority Critical patent/CN114451511B/en
Publication of CN114451511A publication Critical patent/CN114451511A/en
Application granted granted Critical
Publication of CN114451511B publication Critical patent/CN114451511B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention discloses an antibacterial nano-particle and a preparation method and application thereof. The antibacterial nano-particles comprise zein nano-particles and wrapped polyphenol-metal coordination compounds, wherein the polyphenol-metal coordination compounds are obtained by carrying out coordination reaction on polyphenol and iron salt. The preparation method of the antibacterial nano-particles comprises the following steps: 1) preparing zein nanoparticles from zein by an anti-solvent method; 2) adding zein nanoparticles into a polyphenol aqueous solution, and reacting to obtain polyphenol modified nanoparticles; 3) and adding the polyphenol modified nanoparticles into a ferric salt solution to perform coordination reaction. The antibacterial nano-particles can quickly generate high temperature through a photo-thermal effect under the irradiation of near infrared light, achieve a short-time broad-spectrum antibacterial effect, have good biocompatibility, are green and natural in preparation raw materials, are non-toxic, and have wide application prospects in the fields of food antibacterial, medical antibacterial and the like.

Description

Antibacterial nano-particles and preparation method and application thereof
Technical Field
The invention relates to the technical field of food antibiosis, in particular to an antibacterial nanoparticle and a preparation method and application thereof.
Background
In recent decades, due to the use of a large amount of broad-spectrum antibiotics, multi-drug resistant bacteria and highly toxic pathogenic bacteria appear, and the development speed of novel antibiotics is far behind the evolution speed of bacterial drug resistance, so that the clinical antibiotics are difficult to completely meet the requirements at present, and the development of new sterilization products is imminent.
The photothermal antibacterial is a physical sterilization method for effectively killing bacteria by utilizing local heat generated by a photothermal agent under proper light irradiation and through various heat effects of cell membrane rupture, protein/enzyme denaturation, cell cavitation, liquid evaporation in cells and the like. Noble metal (e.g., gold, silver, ruthenium, etc.) nanomaterials have excellent photothermal conversion efficiency and are attracting attention as photothermal agents. Ding et al realized two-photon thermal effect and silver ion release by using positively charged Au-Ag core-shell nanoparticles at wavelength of 808nm and 2W/cm2The effect of killing 92 percent of staphylococcus aureus can be achieved by irradiating for 4min under near infrared laser (NIR). However, the shape, size, etc. of the noble metal nanomaterial greatly affects the photo-thermal performance thereof, and the regulation of the shape, size, and nano structure of the noble metal nanomaterial often requires complex operations and is relatively high in cost, and the noble metal nanomaterial generally has long-term cytotoxicity, and the above factors seriously limit the practical application of the noble metal nanomaterial.
Therefore, the development of the photo-thermal agent with good sterilization effect, no toxicity or harm and good biocompatibility has very important significance.
Disclosure of Invention
The invention aims to provide an antibacterial nano particle and a preparation method and application thereof.
The technical scheme adopted by the invention is as follows:
an antibacterial nano-particle is composed of zein nano-particles and a wrapped polyphenol-metal coordination compound, wherein the polyphenol-metal coordination compound is obtained by carrying out coordination reaction on polyphenol and iron salt.
Preferably, the polyphenol is one of tannic acid, gallic acid, epigallocatechin gallate and procyanidin.
Preferably, the iron salt is at least one of ferric nitrate, ferric chloride and ferric sulfate.
Preferably, the particle size of the antibacterial nano-particles is 122nm to 396 nm.
Preferably, the particle size of the zein nanoparticles is 106 nm-220 nm.
The preparation method of the antibacterial nano-particles comprises the following steps:
1) preparing zein nano particles from zein by an anti-solvent method;
2) adding zein nanoparticles into a polyphenol aqueous solution, and reacting to obtain polyphenol modified nanoparticles;
3) and adding the polyphenol modified nano-particles into an iron salt solution, and performing coordination reaction to obtain the antibacterial nano-particles.
Preferably, the preparation method of the antibacterial nanoparticles comprises the following steps:
1) preparing zein nano particles from zein by an anti-solvent method;
2) adding zein nanoparticles into a polyphenol aqueous solution for reaction, and dialyzing in water to obtain polyphenol modified nanoparticles;
3) and adding the polyphenol modified nano-particles into an iron salt solution, carrying out coordination reaction, and dialyzing in water to obtain the antibacterial nano-particles.
Preferably, the specific operation of preparing zein nanoparticles by an anti-solvent method in the step 1) is as follows: dissolving zein in ethanol water solution, adding water, and removing ethanol to obtain zein nanoparticles.
Further preferably, the specific operation of preparing zein nanoparticles by an anti-solvent method in the step 1) is as follows: dissolving zein in ethanol water solution, adding water in stirring state, and performing rotary evaporation to remove ethanol to obtain zein nanoparticles.
Preferably, the volume fraction of ethanol in the ethanol aqueous solution is 70-90%.
More preferably, the volume fraction of ethanol in the ethanol aqueous solution is 80-90%.
Preferably, the addition ratio of the zein to the ethanol aqueous solution is 30 mg-50 mg:1 mL.
Preferably, the rotary evaporation is carried out at a temperature of 42 ℃ to 48 ℃ and a rotation speed of 150r/min to 180 r/min.
Preferably, the mass ratio of the zein to the polyphenol is 1: 0.04-0.12.
Preferably, the reaction time of the step 2) is 15-40 h.
More preferably, the reaction time in the step 2) is 20 to 30 hours.
Preferably, the molar ratio of the polyphenol to the iron salt is 0.2-2: 1.
Preferably, the coordination reaction in step 3) is carried out under the condition of keeping out light.
Preferably, the time of the coordination reaction in the step 3) is 2 to 10 hours.
Further preferably, the time of the coordination reaction in step 3) is 2 to 6 hours.
The invention has the beneficial effects that: the antibacterial nano-particles can quickly generate high temperature under Near Infrared (NIR) irradiation through a photothermal effect, achieve a short-time broad-spectrum antibacterial effect, have good biocompatibility, are green and natural in preparation raw materials, are non-toxic, and have wide application prospects in the fields of food antibacterial, medical antibacterial and the like.
Specifically, the method comprises the following steps:
1) according to the invention, the zein nanoparticles are modified by polyphenol, then chelation of polyphenol and iron ions is carried out to form a polyphenol-metal coordination compound, and the zein nanoparticles are tightly wrapped by the polyphenol-metal coordination compound to obtain the antibacterial nanoparticles with short-time broad-spectrum antibacterial effect;
2) the antibacterial nano-particles are prepared from green, natural and edible polyphenol, are non-toxic and good in biocompatibility, and can improve the stability and activity of the antibacterial nano-particles;
3) the antibacterial nano-particles break the thalli by utilizing the principle of spontaneously generating high heat by absorbing infrared rays, thereby achieving the effect of high-efficiency broad-spectrum sterilization, also having stronger antibacterial property on drug-resistant bacteria and effectively inhibiting the growth of the drug-resistant bacteria;
4) the preparation method of the antibacterial nano-particles is simple, the preparation conditions are mild, and the preparation method is suitable for large-scale production and application.
Drawings
FIG. 1 is a particle size distribution diagram and an FE-SEM photograph of antibacterial nanoparticles of example 1.
FIG. 2 is a graph showing the photothermal response effect of the antibacterial nanoparticles of examples 1 to 5 and the polyphenol-modified nanoparticles of the comparative example.
FIG. 3 is a graph showing the photothermal bactericidal effect of the antibacterial nanoparticles of examples 1 to 5 and the polyphenol-modified nanoparticles of the comparative example on Escherichia coli.
FIG. 4 is a graph showing the photothermal bactericidal effect of the antibacterial nanoparticles of examples 1 to 5 and the polyphenol-modified nanoparticles of the comparative example on methicillin-resistant Staphylococcus aureus.
FIG. 5 is a graph showing the food medium antibacterial effect of the antibacterial nanoparticles of examples 1 to 3 and polyphenol-modified nanoparticles of comparative example.
Detailed Description
The invention will be further explained and illustrated with reference to specific examples.
Example 1:
an antibacterial nano-particle, the preparation method comprises the following steps:
1) dissolving 1g of zein in 20mL of ethanol aqueous solution (the volume fraction of ethanol is 80%), adding into 50mL of deionized water in a stirring state, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) adding zein nanoparticle water dispersion into 10mL epigallocatechin gallate (EGCG) aqueous solution with concentration of 4mg/mL, reacting at room temperature for 24h, and dialyzing with deionized water at 4 deg.C for 48h to obtain polyphenol modified nanoparticles;
3) adding the polyphenol modified nano-particles into 10mL of ferric chloride solution with the concentration of 2.4mg/mL, stirring for 2h at room temperature in a dark place, and dialyzing for 24h with deionized water at 4 ℃ to obtain the antibacterial nano-particles (the particle size is 122 nm-255 nm).
The particle size distribution and field emission scanning electron microscope (FE-SEM) images of the antibacterial nanoparticles of this example are shown in FIG. 1 (the upper left small image is FE-SEM image).
Testing the particle size distribution: the antibacterial Nano particles are prepared into dispersion liquid with the concentration of 1mg/mL by using deionized water, a proper volume is taken and placed in a cuvette, the particle size of the particles is measured by using a dynamic light scattering Nano particle size meter (Nano-ZS, Malvern), and the measurement result is the average value of three times of measurement.
Testing a field emission scanning electron microscope: and (3) pasting the conductive adhesive on a stainless steel conducting station, fixing the mica sheet by the conductive adhesive, uniformly dropwise adding 10 mu L of antibacterial nano-particle dispersion liquid with the concentration of 0.1mg/mL on the mica sheet, naturally air-drying, carrying out gold spraying treatment, and observing under a scanning electron microscope with the acceleration voltage of 5.0 kV.
As can be seen from fig. 1: the antibacterial nanoparticles of the embodiment are spherical with smooth surface and uniform size, the average particle size is 179.57nm +/-0.33 nm, and the particle sizes of the particles are intensively distributed between 122nm and 255 nm.
Example 2:
an antibacterial nano-particle, the preparation method comprises the following steps:
1) dissolving 1g of zein in 20mL of ethanol aqueous solution (the volume fraction of ethanol is 80%), adding into 50mL of deionized water in a stirring state, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) adding the zein nanoparticle aqueous dispersion into 10mL of EGCG aqueous solution with the concentration of 8mg/mL, reacting at normal temperature for 24h, and dialyzing with deionized water at 4 ℃ for 48h to obtain polyphenol modified nanoparticles;
3) adding the polyphenol modified nano-particles into 10mL of ferric chloride solution with the concentration of 4.8mg/mL, stirring for 2h at room temperature in a dark place, and dialyzing for 24h with deionized water at 4 ℃ to obtain the antibacterial nano-particles (the particle size is 142-295 nm).
Example 3:
an antibacterial nano-particle, the preparation method comprises the following steps:
1) dissolving 1g of zein in 20mL of ethanol aqueous solution (the volume fraction of ethanol is 80%), stirring and adding deionized water until the zein is precipitated, and performing rotary evaporation to remove ethanol under the conditions of 45 ℃ and 150r/min of rotation speed to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) adding the zein nanoparticle aqueous dispersion into 10mL of EGCG aqueous solution with the concentration of 12mg/mL, reacting at normal temperature for 24h, and dialyzing with deionized water at 4 ℃ for 48h to obtain polyphenol modified nanoparticles;
3) adding the polyphenol modified nano-particles into 10mL of ferric chloride solution with the concentration of 7mg/mL, stirring for 2h at room temperature in a dark place, and dialyzing for 24h with deionized water at 4 ℃ to obtain the antibacterial nano-particles (the particle size is 190-396 nm).
Example 4:
an antibacterial nano-particle, the preparation method comprises the following steps:
1) dissolving 1g of zein in 20mL of ethanol aqueous solution (the volume fraction of ethanol is 80%), adding into 50mL of deionized water in a stirring state, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) adding the zein nanoparticle aqueous dispersion into 10mL of EGCG aqueous solution with the concentration of 8mg/mL, reacting at normal temperature for 24h, and dialyzing with deionized water at 4 ℃ for 48h to obtain polyphenol modified nanoparticles;
3) adding the polyphenol modified nano-particles into 10mL of ferric chloride solution with the concentration of 2.4mg/mL, stirring for 2h at room temperature in a dark place, and dialyzing for 24h with deionized water at 4 ℃ to obtain the antibacterial nano-particles (the particle size is 142-295 nm).
Example 5:
an antibacterial nano-particle, the preparation method comprises the following steps:
1) dissolving 1g of zein in 20mL of ethanol aqueous solution (the volume fraction of ethanol is 80%), adding into 50mL of deionized water in a stirring state, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) adding the zein nanoparticle aqueous dispersion into 10mL of EGCG aqueous solution with the concentration of 8mg/mL, reacting at normal temperature for 24h, and dialyzing with deionized water at 4 ℃ for 48h to obtain polyphenol modified nanoparticles;
3) adding the polyphenol modified nano-particles into 10mL of ferric chloride solution with the concentration of 9.5mg/mL, stirring for 2h at room temperature in a dark place, and dialyzing for 24h with deionized water at 4 ℃ to obtain the antibacterial nano-particles (the particle size is 142-295 nm).
Comparative example:
a polyphenol modified nanoparticle, the preparation method comprises the following steps:
1) dissolving 1g of zein in 20mL of ethanol aqueous solution (the volume fraction of ethanol is 80%), adding into 50mL of deionized water in a stirring state, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) adding the zein nanoparticle aqueous dispersion into 10mL of EGCG aqueous solution with the concentration of 8mg/mL, reacting at normal temperature for 24h, and dialyzing with deionized water at 4 ℃ for 48h to obtain the polyphenol modified nanoparticles.
And (3) performance testing:
1) and (3) testing photothermal response: the antibacterial nanoparticles of examples 1-5 and the polyphenol-modified nanoparticles of the comparative example were added with water to prepare 4mg/mL dispersions, 500. mu.L of the dispersion was placed in a centrifuge tube with a volume of 5mL, and then near infrared light (wavelength 808nm, power density 1.5W/cm) was used2) Vertical irradiation centrifuge tube 10And min, recording the temperature change of the solution by an infrared thermal imager at intervals of 60s, and obtaining a photothermal response test result as shown in figure 2.
As can be seen from fig. 2:
a) the temperature of the polyphenol modified nanoparticles of the comparative example is not obviously increased after the polyphenol modified nanoparticles are vertically irradiated by near infrared light for 10min, while the antibacterial nanoparticles of the examples 1 to 5 have an obvious temperature rise phenomenon, which shows that the polyphenol modified nanoparticles cannot generate heat under the irradiation of the near infrared light to increase the temperature, and the antibacterial nanoparticles of the examples 1 to 5 have excellent photo-thermal performance which is endowed by a coordination compound formed after iron ions are introduced;
b) the temperature of the antibacterial nanoparticles in the embodiments 1 to 3 after the antibacterial nanoparticles are vertically irradiated by near infrared light for 10min reaches 42.9 ℃, 59.1 ℃ and 64.8 ℃, which shows that on the premise that the molar ratio of polyphenol to iron ions is consistent, the addition amount of the whole coordination compound (namely polyphenol and ferric chloride) is synchronously increased, the photo-thermal response performance of the system is remarkably improved, and the photo-thermal effect is in direct proportion to the addition amount of the whole coordination compound within a certain range;
c) the temperatures of the antibacterial nanoparticles in the embodiments 4 and 5 reach 52.9 ℃ and 62.0 ℃ respectively after the antibacterial nanoparticles are vertically irradiated by near infrared light for 10min, which shows that the photo-thermal performance and the sterilization effect of the particles are enhanced to a certain extent after the molar ratio of polyphenol to iron ions is reduced under the condition that the addition amount of polyphenol is consistent, and a better antibacterial effect can be obtained on the basis of controlling the cost.
2) Photothermal bactericidal effect test (plate colony counting experiment):
a) centrifuging 5mL of Escherichia Coli (EC) culture solution cultured to logarithmic phase at 4000r/min for 10min, separating bacterial mud, washing with water twice, and resuspending with sterile water to obtain 10% concentration7CFU/mL bacterial suspension, adding 150 μ L bacterial suspension into the same volume of the antibacterial nanoparticle aqueous dispersion (concentration of 4mg/mL) of example 1, mixing well, and first using near infrared light (wavelength of 808nm, power density of 1.5W/cm)2) Standing for 10min, further standing in dark for 10min as illumination group (NIR +), and adding 150 μ L of bacterial suspension into the same volume of antibacterial nanometer of example 1Uniformly mixing the particle aqueous dispersion (the concentration is 4mg/mL), standing and incubating for 20min in a dark environment to serve as a non-illumination group (marked as NIR-), and replacing the antibacterial nanoparticle aqueous dispersion with sterile water for blank control;
b) performing the same operation with methicillin-resistant Staphylococcus aureus (MRSA) culture solution, and diluting the bacterial suspension to 104CFU/mL, 100 μ L of the mixture is uniformly coated on an LB plate, the mixture is placed in a constant temperature incubator at 37 ℃ for 24 hours, the observation and record are carried out, the colony count of the plate is carried out, the survival amount of bacteria treated under different conditions is calculated through log10(CFU/mL), each sample to be tested is repeated three times, the same test is carried out on the antibacterial nanoparticles of examples 2 to 5 and the polyphenol modified nanoparticles of the comparative example, the photo-thermal sterilization effect graph of the antibacterial nanoparticles of examples 1 to 5 and the polyphenol modified nanoparticles of the comparative example on escherichia coli is shown in figure 3, and the photo-thermal sterilization effect graph of the antibacterial nanoparticles of examples 1 to 5 and the polyphenol modified nanoparticles of the comparative example on methicillin-resistant staphylococcus aureus is shown in figure 4.
As can be seen from fig. 3 and 4:
the bacteria acted on the polyphenol-modified nanoparticles of the comparative example showed no significant difference in colony numbers after NIR + treatment from the NIR-treated and blank control treatment, indicating that the antibacterial effect of the polyphenol-modified nanoparticles alone was insignificant, whereas the bacteria acted on the antibacterial nanoparticles of example 1 showed log of EC and MRSA after NIR + treatment10The (CFU/mL) values were reduced by about 0.12 and 0.67 compared to the blank, respectively, and the bacteria with antibacterial nanoparticle effect of example 2 did not grow in the NIR + treated MRSA and the EC slightly grew as colonies, the log of which10(CFU/mL) value dropped to 3.56. + -. 0.36, with no colony growth at all in both the NIR + treated EC and MRSA for the antibacterial nanoparticle-affected bacteria of example 3, and log of EC and MRSA after NIR + treatment for the antibacterial nanoparticle-affected bacteria of example 410The (CFU/mL) values were reduced by about 0.52 and 2.41 compared to the blank, respectively, and no colony growth was observed at all for the bacteria of example 5 with antibacterial nanoparticle effect in EC and MRSA after NIR + treatment. As can be seen, the antibacterial nanoparticles of examples 1-5 have a broad bactericidal effect on MRSAIs superior to EC, and shows excellent bactericidal performance for drug-resistant bacteria. In addition, under the same NIR treatment time, the sterilization effect of the antibacterial nanoparticles is in positive correlation with the photothermal response temperature of the antibacterial nanoparticles, and the excellent antibacterial effect of the antibacterial nanoparticles is fully shown to be derived from the photothermal response performance of the antibacterial nanoparticles.
3) And (3) testing the antibacterial effect of the food matrix: centrifuging 5mL MRSA culture solution cultured to logarithmic phase at 4000r/min for 10min, separating bacterial sludge, washing with water twice, and resuspending with sterile water to obtain 10% concentration7CFU/mL bacterial suspension, taking fresh beef with the size specification of 2cm × 2cm × 1cm, respectively adding 15 μ L bacterial suspension and equal volume of the antibacterial nanoparticle aqueous dispersion (concentration of 4mg/mL) of example 1 on the surface of the fresh beef, uniformly mixing, and firstly performing near infrared light (wavelength of 808nm and power density of 1.5W/cm)2) Standing and incubating for 10min, standing and incubating for 10min in dark environment as illumination group (marked as NIR +), taking fresh beef with size specification of 2cm × 2cm × 1cm, adding 15 μ L of bacterial suspension and equal volume of the antibacterial nanoparticle aqueous dispersion (concentration is 4mg/mL) of example 1 on the surface of the fresh beef, mixing uniformly, standing and incubating for 20min in dark environment as non-illumination group (marked as NIR-), adopting sterile water as a blank control to replace the antibacterial nanoparticle aqueous dispersion, respectively filling the beef into conical flasks containing 15mL of LB broth culture medium, placing in a constant temperature gas bath shaker for shaking culture at 37 ℃ for 7h, taking 100 μ L of culture medium diluted by 1000 times, coating on the uniform LB broth agar culture medium, placing the plate in a constant temperature incubator for culturing at 37 ℃ for 24h, observing and recording, and counting plate colonies, the antibacterial survival rates of bacteria were measured, each sample was repeated three times, the same test was performed on the antibacterial nanoparticles of examples 2 to 3 and the polyphenol-modified nanoparticles of comparative example, and the food medium antibacterial effect graphs of the obtained antibacterial nanoparticles of examples 1 to 3 and the polyphenol-modified nanoparticles of comparative example are shown in fig. 5.
The bacterial survival rate calculation formula is as follows: bacterial survival (%) — number of colonies on the sample-treated plate/number of colonies on the blank control plate × 100%.
As can be seen from fig. 5: the survival rates of the bacteria of the MRSA acted by the polyphenol modified nanoparticles of the comparative example are not reduced, but the survival rates of the bacteria of the MRSA acted by the antibacterial nanoparticles of the examples 1 to 3 are respectively reduced to 31.31% +/-0.64%, 0.57% +/-0.20% and 0.08% +/-0.06% after the antibacterial nanoparticles are subjected to NIR + treatment, which shows that the antibacterial nanoparticles still have good antibacterial effect in food media, and have the development potential of being used as a short-term broad-spectrum antibacterial agent which is green, natural and good in effect in the processes of food production and packaging.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (10)

1. An antimicrobial nanoparticle comprising a zein nanoparticle and an encapsulated polyphenol-metal complex; the polyphenol-metal coordination compound is obtained by carrying out coordination reaction on polyphenol and iron salt.
2. The antimicrobial nanoparticle of claim 1, wherein: the polyphenol is one of tannic acid, gallic acid, epigallocatechin gallate and procyanidin.
3. Antibacterial nanoparticles according to claim 1 or 2, characterized in that: the ferric salt is at least one of ferric nitrate, ferric chloride and ferric sulfate.
4. Antibacterial nanoparticles according to claim 1 or 2, characterized in that: the particle size of the antibacterial nano-particles is 122 nm-396 nm.
5. Antibacterial nanoparticles according to claim 1 or 2, characterized in that: the particle size of the zein nano particles is 106 nm-220 nm.
6. The method for preparing antibacterial nanoparticles according to any one of claims 1 to 5, characterized by comprising the steps of:
1) preparing zein nano particles from zein by an anti-solvent method;
2) adding zein nanoparticles into a polyphenol aqueous solution, and reacting to obtain polyphenol modified nanoparticles;
3) and adding the polyphenol modified nano-particles into an iron salt solution, and performing coordination reaction to obtain the antibacterial nano-particles.
7. The method of claim 6, wherein the antimicrobial nanoparticles are prepared by: the specific operation of preparing zein nanoparticles by an anti-solvent method in the step 1) is as follows: dissolving zein in ethanol water solution, adding water, and removing ethanol to obtain zein nanoparticles.
8. The method for preparing antibacterial nanoparticles according to claim 6 or 7, characterized in that: the mass ratio of the zein to the polyphenol is 1: 0.04-0.12.
9. The method for preparing antibacterial nanoparticles according to claim 6 or 7, characterized in that: the molar ratio of the polyphenol to the iron salt is 0.2-2: 1.
10. Use of antibacterial nanoparticles according to any one of claims 1 to 5 in the preparation of a photothermal agent.
CN202210008131.9A 2022-01-05 2022-01-05 Antibacterial nano-particles and preparation method and application thereof Active CN114451511B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210008131.9A CN114451511B (en) 2022-01-05 2022-01-05 Antibacterial nano-particles and preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210008131.9A CN114451511B (en) 2022-01-05 2022-01-05 Antibacterial nano-particles and preparation method and application thereof

Publications (2)

Publication Number Publication Date
CN114451511A true CN114451511A (en) 2022-05-10
CN114451511B CN114451511B (en) 2023-09-26

Family

ID=81409929

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210008131.9A Active CN114451511B (en) 2022-01-05 2022-01-05 Antibacterial nano-particles and preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN114451511B (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114948996A (en) * 2022-06-13 2022-08-30 国家纳米科学中心 Antibacterial agent and preparation method and application thereof
CN115093614A (en) * 2022-06-28 2022-09-23 江南大学 Protective starch-based film and preparation method and application thereof
CN115651276A (en) * 2022-09-29 2023-01-31 江苏省农业科学院 Chitosan composite membrane containing protein nanoparticles and preparation method and application thereof

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103734742A (en) * 2014-01-24 2014-04-23 上海理工大学 Polyphenols-hordein nanoparticle and preparation method thereof
CN108066761A (en) * 2017-12-30 2018-05-25 广西师范大学 A kind of preparation method and applications of metal-polyphenol nano-particle
CN109676149A (en) * 2018-12-13 2019-04-26 华南理工大学 A kind of wheat gliadin/nano-Ag particles of green syt core-shell structure and the preparation method and application thereof
CN111420064A (en) * 2020-04-21 2020-07-17 华南理工大学 protein-EGCG composite nanoparticle and antioxidant Pickering high internal phase emulsion
CN111534110A (en) * 2020-05-29 2020-08-14 华南农业大学 Prolamin-phenol antioxidant nanoparticle and pickering emulsion prepared from prolamin-phenol antioxidant nanoparticle
CN112121178A (en) * 2020-10-29 2020-12-25 江南大学 Water-soluble zein-EGCG covalent compound and preparation and application thereof
CN112791185A (en) * 2021-01-20 2021-05-14 广州医科大学 Nano medicine for treating tumor by combining photothermal treatment with iron agent and preparation method thereof
CN113016823A (en) * 2021-02-02 2021-06-25 南京师范大学 Preparation method of photo-thermal antibacterial near-infrared bimetallic nanoparticles
CN113185732A (en) * 2021-04-30 2021-07-30 西北农林科技大学 Pectin-based nano-melanin edible photothermal antibacterial film and preparation method and application thereof
CN113289014A (en) * 2021-05-10 2021-08-24 上海交通大学 Zwitterionic polypeptide/gallic acid/iron coordination nanoparticle with ultra-small particle size as well as preparation method and application thereof
CN113647607A (en) * 2021-08-06 2021-11-16 广州大学 Mineral-loaded ovalbumin-polyphenol nanoparticles and preparation method and application thereof

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103734742A (en) * 2014-01-24 2014-04-23 上海理工大学 Polyphenols-hordein nanoparticle and preparation method thereof
CN108066761A (en) * 2017-12-30 2018-05-25 广西师范大学 A kind of preparation method and applications of metal-polyphenol nano-particle
CN109676149A (en) * 2018-12-13 2019-04-26 华南理工大学 A kind of wheat gliadin/nano-Ag particles of green syt core-shell structure and the preparation method and application thereof
CN111420064A (en) * 2020-04-21 2020-07-17 华南理工大学 protein-EGCG composite nanoparticle and antioxidant Pickering high internal phase emulsion
CN111534110A (en) * 2020-05-29 2020-08-14 华南农业大学 Prolamin-phenol antioxidant nanoparticle and pickering emulsion prepared from prolamin-phenol antioxidant nanoparticle
CN112121178A (en) * 2020-10-29 2020-12-25 江南大学 Water-soluble zein-EGCG covalent compound and preparation and application thereof
CN112791185A (en) * 2021-01-20 2021-05-14 广州医科大学 Nano medicine for treating tumor by combining photothermal treatment with iron agent and preparation method thereof
CN113016823A (en) * 2021-02-02 2021-06-25 南京师范大学 Preparation method of photo-thermal antibacterial near-infrared bimetallic nanoparticles
CN113185732A (en) * 2021-04-30 2021-07-30 西北农林科技大学 Pectin-based nano-melanin edible photothermal antibacterial film and preparation method and application thereof
CN113289014A (en) * 2021-05-10 2021-08-24 上海交通大学 Zwitterionic polypeptide/gallic acid/iron coordination nanoparticle with ultra-small particle size as well as preparation method and application thereof
CN113647607A (en) * 2021-08-06 2021-11-16 广州大学 Mineral-loaded ovalbumin-polyphenol nanoparticles and preparation method and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
赵宇欢: "光热纳米材料的制备及其在抗菌领域的应用", <中国优秀硕士论文电子期刊网> *
邢雅艳等: "儿茶素-银纳米复合材料的制备及其应用", 《应用化学》 *
鄢俐等: "负载小麦醇溶蛋白纳米银胶体颗粒的抗菌壳聚糖复合膜构建及表征", 《现代食品科技》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114948996A (en) * 2022-06-13 2022-08-30 国家纳米科学中心 Antibacterial agent and preparation method and application thereof
CN115093614A (en) * 2022-06-28 2022-09-23 江南大学 Protective starch-based film and preparation method and application thereof
CN115651276A (en) * 2022-09-29 2023-01-31 江苏省农业科学院 Chitosan composite membrane containing protein nanoparticles and preparation method and application thereof

Also Published As

Publication number Publication date
CN114451511B (en) 2023-09-26

Similar Documents

Publication Publication Date Title
CN114451511B (en) Antibacterial nano-particles and preparation method and application thereof
de Faria et al. Eco-friendly decoration of graphene oxide with biogenic silver nanoparticles: antibacterial and antibiofilm activity
Xu et al. Role of reactive oxygen species in the antibacterial mechanism of silver nanoparticles on Escherichia coli O157: H7
CN108042565B (en) Black phosphorus nano material with antibacterial effect and preparation method thereof
Safaei et al. Optimized synthesis, characterization, and antibacterial activity of an alginate–cupric oxide bionanocomposite
CN114306382B (en) Copper-based nanoenzyme as well as preparation method and application thereof
CN111011393A (en) Preparation of simulated oxidase and photocatalytic bacteriostatic and bactericidal application thereof
Qian et al. 3D-printed CuFe2O4-MXene/PLLA antibacterial tracheal scaffold against implantation-associated infection
Kiprono et al. Encapsulation of E. coli in biomimetic and Fe 3 O 4-doped hydrogel: structural and viability analyses
Acharya et al. Physicochemical and antimicrobial properties of sodium alginate/gelatin-based silver nanoformulations
CN112056310B (en) DFNS (double-walled carbon nanotubes) loaded carbon quantum dot/molybdenum disulfide quantum dot as well as preparation method and application thereof
CN108208003A (en) A kind of Ag/ dopamines/g-C3N4Visible light catalytic fungicide
Wansapura et al. Preparation of chitin‐CdTe quantum dots films and antibacterial effect on Staphylococcus aureus and Pseudomonas aeruginosa
Rao et al. Polyethyleneimine induced highly dispersed Ag nanoparticles over g-C3N4 nanosheets for efficient photocatalytic and antibacterial performance
Jia et al. Silver nanoparticles anchored magnetic self-assembled carboxymethyl cellulose-ε-polylysine hybrids with synergetic antibacterial activity for wound infection therapy
Kuang et al. Biomimetic FeCo@ PDA nanozyme platform with Fenton catalytic activity as efficient antibacterial agent
Guo et al. Electrophoretic deposition of photothermal responsive antibacterial coatings on titanium with controlled release of silver ions
CN115414478B (en) Preparation method of antibacterial light response composite material
Ameen et al. Excellent antimicrobial performances of Cu (II) metal organic framework@ Fe3O4 fused cubic particles
Rashid et al. Characterization and antimicrobial efficiency of silver nanoparticles based reduction method
Amaravathi et al. Biopolymer-PAA and surfactant-CTAB assistant solvothermal synthesis of Mn-based MOFs: design, characterization for enhanced biological activities
CN114469893A (en) Quaternary ammonium salinized silicon dioxide nano-particles, preparation method and application
Long et al. Preparation and Antimicrobial Activity of Antibacterial Silver-Loaded Polyphosphazene Microspheres
CN110625132A (en) Sulfhydrylation amphiphilic carbon residue/nano-silver compound, preparation method thereof and antibacterial leather
CN116326594B (en) Composite material for ocean corrosion prevention and pollution prevention as well as preparation method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant