CN114414568A - Microfluidic liquid crystal sensor capable of detecting organophosphorus pesticide in real time and detection method thereof - Google Patents
Microfluidic liquid crystal sensor capable of detecting organophosphorus pesticide in real time and detection method thereof Download PDFInfo
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- CN114414568A CN114414568A CN202210016386.XA CN202210016386A CN114414568A CN 114414568 A CN114414568 A CN 114414568A CN 202210016386 A CN202210016386 A CN 202210016386A CN 114414568 A CN114414568 A CN 114414568A
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- 239000004973 liquid crystal related substance Substances 0.000 title claims abstract description 52
- 238000001514 detection method Methods 0.000 title claims abstract description 45
- 239000003987 organophosphate pesticide Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000011521 glass Substances 0.000 claims description 38
- 239000000243 solution Substances 0.000 claims description 32
- 238000001035 drying Methods 0.000 claims description 26
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 21
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 21
- 239000008367 deionised water Substances 0.000 claims description 19
- 229910021641 deionized water Inorganic materials 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims description 18
- 238000004528 spin coating Methods 0.000 claims description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 239000000575 pesticide Substances 0.000 claims description 14
- 238000007872 degassing Methods 0.000 claims description 12
- 239000000428 dust Substances 0.000 claims description 12
- 229910052751 metal Inorganic materials 0.000 claims description 12
- 239000002184 metal Substances 0.000 claims description 12
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims description 12
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 10
- 238000004140 cleaning Methods 0.000 claims description 10
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 10
- 239000012498 ultrapure water Substances 0.000 claims description 10
- 238000005406 washing Methods 0.000 claims description 9
- 238000005520 cutting process Methods 0.000 claims description 8
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 7
- 229910052710 silicon Inorganic materials 0.000 claims description 7
- 239000010703 silicon Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 229910001914 chlorine tetroxide Inorganic materials 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- -1 polydimethylsiloxane Polymers 0.000 claims description 5
- 238000004080 punching Methods 0.000 claims description 5
- 230000021523 carboxylation Effects 0.000 claims description 4
- 238000006473 carboxylation reaction Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000009832 plasma treatment Methods 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 3
- 239000005562 Glyphosate Substances 0.000 description 6
- 229940097068 glyphosate Drugs 0.000 description 6
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 6
- 239000005949 Malathion Substances 0.000 description 5
- JXSJBGJIGXNWCI-UHFFFAOYSA-N diethyl 2-[(dimethoxyphosphorothioyl)thio]succinate Chemical compound CCOC(=O)CC(SP(=S)(OC)OC)C(=O)OCC JXSJBGJIGXNWCI-UHFFFAOYSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 229960000453 malathion Drugs 0.000 description 5
- 238000002156 mixing Methods 0.000 description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 238000011010 flushing procedure Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- ZRGUXTGDSGGHLR-UHFFFAOYSA-K aluminum;triperchlorate Chemical compound [Al+3].[O-]Cl(=O)(=O)=O.[O-]Cl(=O)(=O)=O.[O-]Cl(=O)(=O)=O ZRGUXTGDSGGHLR-UHFFFAOYSA-K 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- 238000009987 spinning Methods 0.000 description 2
- 102000012440 Acetylcholinesterase Human genes 0.000 description 1
- 108010022752 Acetylcholinesterase Proteins 0.000 description 1
- 229940022698 acetylcholinesterase Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 210000002858 crystal cell Anatomy 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000004885 tandem mass spectrometry Methods 0.000 description 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
Abstract
The invention discloses a micro-fluidic liquid crystal sensor capable of detecting organophosphorus pesticide in real time and a detection method thereof. The method combines the liquid crystal detection technology with the micro-fluidic technology, and realizes the real-time, rapid and convenient detection of the organophosphorus pesticide.
Description
Technical Field
The invention relates to the fields of liquid crystal detection technology, microfluidics and organophosphorus pesticide detection, in particular to a microfluidic liquid crystal sensor capable of detecting organophosphorus pesticide in real time and a detection method thereof.
Background
The organophosphorus pesticide has the characteristics of high efficiency and broad spectrum, thus becoming one of the most widely used pesticides in agriculture at present. However, organophosphorus inhibits the activity of acetylcholinesterase, thus causing pathological changes and even death of the organism. In recent years, the excessive use of pesticides causes serious pesticide residue problems, particularly residues on fruits and vegetables and other foods seriously threaten the health of people, and great food safety problems are caused. Therefore, the method has great significance for detecting the organophosphorus pesticide residue in real time.
As methods for detecting organophosphorus pesticides, High Performance Liquid Chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS), Gas Chromatography (GC), gas chromatography-mass spectrometry (GC-MS), gas chromatography-tandem mass spectrometry (GC-MS/MS), and the like have been reported. However, these methods require complicated sample handling, specialized laboratory personnel and sophisticated equipment, are time consuming to operate, and are not suitable for real-time, rapid detection.
In the existing research, the liquid crystal sensor has attracted people's attention due to simple operation, sensitive reaction and low sample consumption. The micro-fluidic device can be used for realizing a miniaturized experimental process and has the characteristics of short detection time and low cost. However, the existing microfluidic detection design still needs complex signal detection and enhancement methods or devices to assist in reading the detection result. To solve these problems, the present invention combines the two and reports the molecules detected in the microfluidic device by using liquid crystal, which is a significant and promising task.
Disclosure of Invention
In order to realize the purpose, the invention adopts the following technical scheme:
a microfluidic liquid crystal sensor for detecting organophosphorus pesticide is formed by assembling a PDMS micro-channel on a glass slide fixed by metal perchlorate; and the micro-channel is injected with organophosphorus pesticide and liquid crystal.
Further, the height of the micro-channel is 10-50 μm, and the width is 100-500 μm; the metal perchlorate is Cu (ClO)4)2，Al(ClO4)3，Zn(ClO4)2，Fe(ClO4)3One kind of (1).
A detection method of a liquid crystal sensor for visually detecting organophosphorus pesticide comprises the following steps:
1) pretreatment of slides
And soaking the cut glass slide in a cleaning solution, cleaning and drying.
2) Carboxylation treatment of glass slide
3) Fixing glass slide with metal perchlorate
Dripping anhydrous alcohol solution of metal perchlorate on the center of the surface of the glass slide treated in the step 2), spin-coating the ethanol solution of metal perchlorate on the glass slide according to set spin-coating parameters, drying and preventing dust for later use
4) Preparation and cleaning of PDMS (polydimethylsiloxane) micro-channel
5) Assembly of microfluidic devices
Combining the slide obtained in the step 3) with the PDMS microchannel obtained in the step 4) after plasma treatment to form the required microfluidic device
6) Assembly of microfluidic liquid crystal sensor
Respectively injecting organophosphorus pesticide, deionized water and liquid crystal into the assembled device in the step 5) along the punched holes, and staying for 30 minutes every time when one substance is injected
In the detection method, furthermore, in the step 1), the glass slide is soaked in 1-10% (V/V) decon-90 aqueous solution for 2-12h, then the glass slide is cleaned by ultrapure water and treated by N2Drying and preventing dust for later use; the step 2) of carboxylation treatment of the glass slide is to soak the glass slide pretreated in the step 1) with TESPSA solution at 60-100 ℃, rinse the glass slide with ultrapure water, and perform N treatment2And drying and preventing dust for later use.
In the detection method, the volume fraction of the TESPSA solution is 0-1%, and the soaking time is 2-8 h.
In the above detection method, further, the spin coating parameter in step 3) is set to 2000-6000rpm, the spin coating time is 0.5-2min, the concentration of metal perchlorate is 5-20mM, and the spin coating dosage is 30-100 μ L.
In the detection method, the preparation and cleaning of the PDMS microchannel in the step 4) are specifically performed by mixing a PDMS prepolymer main agent and a curing agent in an amount of 10:1(w/w), stirring and degassing, transferring the degassed mixture onto a silicon master plate, degassing again, curing in an oven at 80-110 ℃, and removing the microchannel from the silicon master plate. Cutting and punching to form a micro-channel with a pore channel, washing the micro-channel in isopropanol, drying the micro-channel, and treating the micro-channel by using a plasma process;
the stirring time is 5-15min, the degassing time is 10-30min, and the curing time is 2-6 h.
In the detection method, further, the plasma processing time in the step 5) is 0.5-5min, the power is 50-255w, and the oxygen amount is 2-6 sccm.
In the detection method, further, the concentration of the organophosphorus pesticide solution in the step 6) is 1-50 μ g/mL, and the amounts of the liquid crystal, the deionized water and the organophosphorus pesticide solution which are absorbed are 2-6 μ L, 4-12 μ L and 2-6 μ L respectively.
The invention provides an application of the microfluidic liquid crystal sensor in organophosphorus pesticide detection.
Compared with the prior art, the invention has the beneficial effects that:
the invention successfully combines the micro-fluidic device with the liquid crystal for detecting the organophosphorus pesticide, can detect the organophosphorus pesticide in real time and rapidly, has low sample consumption and no need of additional treatment, and solves the problem of complex operation of the existing detection method.
Drawings
FIG. 1 is a schematic diagram of a liquid crystal microfluidic device for detecting organophosphorus pesticide
FIG. 2 shows 20mM Al (ClO)4)3Modified liquid crystal optical photos of microfluidic devices with different sizes
FIG. 3 is a photograph showing the results of detection in example 2
FIG. 4 is a photograph showing the results of detection in example 3
Detailed Description
The treatment process of the present invention is further illustrated below with reference to specific examples.
Example 1
1) Cutting the slide toSoaking in decon-90 solution, washing with ultrapure water, and treating with N2Drying, fully drying, and preventing dust for later use;
2) immersing the glass slide pretreated in the step 1) in a TESPSA solution, fully immersing at 80 ℃, washing with ultrapure water, and carrying out N2Drying, fully drying, and preventing dust for later use;
3) 20mM of Al (ClO) was taken4)3Dripping anhydrous ethanol solution into the center of the surface of the glass slide subjected to carboxyl treatment in the step 2), and carrying out spin coating on Al (ClO) according to set spin coating parameters4)3Spin-coating on glass slide, drying, and keeping dustproof.
4) Mixing a Polydimethylsiloxane (PDMS) prepolymer main agent and a curing agent according to the amount of 10:1(w/w), stirring and degassing, transferring the degassed mixture onto a silicon master plate, degassing again, and curing in an oven at 80 ℃. And cutting and punching the micro-channel, washing the micro-channel in isopropanol, and drying the micro-channel.
5) Combining the slide obtained in the step 3) with the PDMS micro-channel obtained in the step 4) which is processed by the plasma to form the required micro-fluidic device.
6) Respectively sucking the liquid crystal, the deionized water and the glyphosate pesticide solution by using an injector, then sequentially injecting the liquid crystal, the deionized water and the glyphosate pesticide solution into the device assembled in the step 5) along the punched holes, respectively staying for 30 minutes, flushing away the redundant pesticide by using the deionized water after injecting the deionized water, extruding away the deionized water by using the liquid crystal after injecting the liquid crystal, and finally only remaining the liquid crystal in the channel for observation.
Since the liquid crystal in the microchannel is in contact with the channel walls of four different length scales, rather than two surfaces of the liquid crystal cell, its orientation depends not only on its surface function but also on the geometry of the microchannel, so its size is first of all investigated for optimization. Four different sizes of microchannels were selected, with a height of 20 μm and 50 μm combined with a width of 200 μm and 500 μm, respectively. Specific parameters and results are shown in FIG. 2, which shows that on a 20mM aluminum perchlorate modified substrate, the induction effect on a microchannel with a height of 20 μm is obvious, and the induction effect on a microchannel with a height of 50 μm is poor, so that the microchannel with a height of 20 μm is used as a detection device as a preferred microchannel design.
Example 2
1) Cutting the glass slide to the required size, fully soaking the glass slide in prepared decon-90 solution, then cleaning the glass slide by using ultrapure water, and carrying out N-step treatment2Drying, fully drying, and preventing dust for later use;
2) immersing the glass slide pretreated in the step 1) in a TESPSA solution, fully immersing at 80 ℃, washing with ultrapure water, and carrying out N2Drying, fully drying, and preventing dust for later use;
3) taking 8-10mM Al (ClO)4)3Dripping anhydrous ethanol solution into the center of the surface of the glass slide subjected to carboxyl treatment in the step 2), and carrying out spin coating on Al (ClO) according to set spin coating parameters4)3The ethanol solution is coated on a glass slide in a spinning way, dried and dustproof for standby.
4) Mixing a Polydimethylsiloxane (PDMS) prepolymer main agent and a curing agent according to the amount of 10:1(w/w), stirring and degassing, transferring the degassed mixture onto a silicon master plate, degassing again, and curing in an oven at 80 ℃. And cutting and punching the micro-channel, washing the micro-channel in isopropanol, and drying the micro-channel.
5) Combining the slide obtained in the step 3) with the PDMS micro-channel obtained in the step 4) which is processed by the plasma to form the required micro-fluidic device.
6) Respectively sucking the liquid crystal, the deionized water and the glyphosate pesticide by using an injector, then sequentially injecting the liquid crystal, the deionized water and the glyphosate pesticide into the device assembled in the step 5) along the punched holes, respectively staying for 30 minutes, flushing away the redundant glyphosate pesticide by using the deionized water after injecting the deionized water, extruding away the deionized water by using the liquid crystal after injecting the liquid crystal, and finally only remaining the liquid crystal in the channel for observation.
FIG. 1 is a schematic diagram of a liquid crystal microfluidic device for detecting organophosphorus pesticide. The specific detection results are shown in fig. 3, and the micro-channels with the height of 20 μm have different liquid crystal inductivity and different pesticide detection effects due to the aluminum perchlorate with different widths. In which a 500 μm wide channel was spin coated with 8mM Al (ClO)4)3Under the condition of ethanol solution, the performance is better, the detection of the glyphosate can be realized, and the detection limit reaches 20 mug/mL.
Example 3
1) Cutting the glass slide to the required size, fully soaking the glass slide in prepared decon-90 solution, then cleaning the glass slide by using ultrapure water, and carrying out N-step treatment2Drying, fully drying, and preventing dust for later use;
2) immersing the glass slide pretreated in the step 1) in a TESPSA solution, fully immersing at 80 ℃, washing with ultrapure water, and carrying out N2Drying, fully drying, and preventing dust for later use;
3) take 8mM Al (ClO)4)3Dripping anhydrous ethanol solution into the center of the surface of the glass slide subjected to carboxyl treatment in the step 2), and carrying out spin coating on Al (ClO) according to set spin coating parameters4)3The ethanol solution is coated on a glass slide in a spinning way, dried and dustproof for standby.
4) Mixing a Polydimethylsiloxane (PDMS) prepolymer main agent and a curing agent according to the amount of 10:1(w/w), stirring and degassing, transferring the degassed mixture onto a silicon master plate, degassing again, and curing in an oven at 80 ℃. And cutting and punching the micro-channel, washing the micro-channel in isopropanol, and drying the micro-channel.
5) Combining the slide obtained in the step 3) with the PDMS micro-channel obtained in the step 4) which is processed by the plasma to form the required micro-fluidic device.
6) Respectively sucking the liquid crystal, the deionized water and the malathion pesticide solution by using an injector, then sequentially injecting the liquid crystal, the deionized water and the malathion pesticide solution into the device assembled in the step 5) along the punched holes, respectively staying for 30 minutes, flushing away the redundant malathion pesticide by using the deionized water after injecting the deionized water, extruding away the deionized water by using the liquid crystal after injecting the liquid crystal, and finally only remaining the liquid crystal in the channel for observation.
The specific detection result is shown in fig. 4, the detection effect of the invention on malathion is better, the detection on malathion can be realized, and the detection limit reaches 1 mug/mL.
It will be apparent to those skilled in the art from this disclosure that many changes and modifications can be made, or equivalents modified, in the embodiments of the invention without departing from the scope of the invention. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention shall still fall within the protection scope of the technical solution of the present invention, unless the contents of the technical solution of the present invention are departed.
Claims (10)
1. The microfluidic liquid crystal sensor for detecting the organophosphorus pesticide is characterized in that the liquid crystal sensor is formed by assembling a PDMS micro-channel loaded on a glass slide fixed by metal perchlorate; and the micro-channel is injected with organophosphorus pesticide and liquid crystal.
2. The microfluidic liquid crystal sensor for detecting organophosphorus pesticide as claimed in claim 1, wherein the height of the microchannel is 10-50 μm, and the width of the microchannel is 100-500 μm; the metal perchlorate is Cu (ClO)4)2，Al(ClO4)3，Zn(ClO4)2，Fe(ClO4)3One kind of (1).
3. The detection method of the liquid crystal sensor for visually detecting the organophosphorus pesticide as described in claim 1-2, is characterized by comprising the following steps:
1) pretreatment of slides
Soaking the cut glass slide in a cleaning solution, cleaning and drying;
2) carboxylation treatment of glass slide
3) Fixing glass slide with metal perchlorate
Dripping anhydrous alcohol solution of metal perchlorate on the center of the surface of the glass slide treated in the step 2), spin-coating the ethanol solution of metal perchlorate on the glass slide according to set spin-coating parameters, drying and preventing dust for later use;
4) preparation and cleaning of PDMS (polydimethylsiloxane) micro-channel
5) Assembly of microfluidic devices
Combining the glass slide obtained in the step 3) with the PDMS microchannel obtained in the step 4) after plasma treatment to form a required microfluidic device;
6) assembly of microfluidic liquid crystal sensor
And (3) respectively injecting organophosphorus pesticide, deionized water and liquid crystal into the assembled device in the step 5) along the punched holes, and staying for 30 minutes every time one substance is injected.
4. The detection method of the microfluidic liquid crystal sensor capable of detecting the organophosphorus pesticide in real time according to claim 3, wherein the detection method comprises the following steps: in the step 1), the glass slide is soaked in 1-10% (V/V) decon-90 aqueous solution for 2-12h, then the glass slide is cleaned by ultrapure water and treated by N2Drying and preventing dust for later use; the step 2) of carboxylation treatment of the glass slide is to soak the glass slide pretreated in the step 1) with TESPSA solution at 60-100 ℃, rinse the glass slide with ultrapure water, and perform N treatment2And drying and preventing dust for later use.
5. The detection method of the microfluidic liquid crystal sensor capable of detecting the organophosphorus pesticide in real time according to claim 4, wherein the detection method comprises the following steps: the volume fraction of the TESPSA solution is 0-1%, and the soaking time is 2-8 h.
6. The detection method of the microfluidic liquid crystal sensor capable of detecting the organophosphorus pesticide in real time according to claim 3, wherein the detection method comprises the following steps: the spin coating parameter in the step 3) is set to 2000-6000rpm, the spin coating time is 0.5-2min, the concentration of metal perchlorate is 5-20mM, and the spin coating dosage is 30-100 muL.
7. The detection method of the microfluidic liquid crystal sensor capable of detecting the organophosphorus pesticide in real time according to claim 3, wherein the detection method comprises the following steps: the preparation and cleaning of the PDMS microchannel in the step 4) are specifically that a PDMS prepolymer main agent and a curing agent are mixed according to the amount of 10:1(w/w), stirring and degassing are carried out, the degassed mixture is transferred to a silicon master plate, degassing is carried out again, curing is carried out in an oven at the temperature of 80-110 ℃, and the microchannel is taken down from the silicon template. Cutting and punching to form a micro-channel with a hollow pore channel, washing the micro-channel in isopropanol, drying the micro-channel, and treating the micro-channel by using a plasma process;
the stirring time is 5-15min, the degassing time is 10-30min, and the curing time is 2-6 h.
8. The detection method of the microfluidic liquid crystal sensor capable of detecting the organophosphorus pesticide in real time according to claim 3, wherein the detection method comprises the following steps: the plasma treatment time in the step 5) is 0.5-5min, the power is 50-255w, and the oxygen amount is 2-6 sccm.
9. The detection method of the microfluidic liquid crystal sensor capable of detecting the organophosphorus pesticide in real time according to claim 3, wherein the detection method comprises the following steps: the concentration of the organophosphorus pesticide solution in the step 6) is 1-50 mu g/mL, and the absorbed liquid crystal, deionized water and organophosphorus pesticide solution are 2-6 mu L, 4-12 mu L and 2-6 mu L respectively.
10. Use of the microfluidic liquid crystal sensor according to any one of claims 1-2 for the detection of organophosphorus pesticides.
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| CN115322982A (en) * | 2022-08-15 | 2022-11-11 | 北京工商大学 | Preparation method and application of cell-loaded microcapsule |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115322982A (en) * | 2022-08-15 | 2022-11-11 | 北京工商大学 | Preparation method and application of cell-loaded microcapsule |
| CN115322982B (en) * | 2022-08-15 | 2023-08-15 | 北京工商大学 | Preparation method and application of cell-loaded microcapsule |
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