CN114413209B - Monitoring device and dissociation device - Google Patents
Monitoring device and dissociation device Download PDFInfo
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- CN114413209B CN114413209B CN202210315864.7A CN202210315864A CN114413209B CN 114413209 B CN114413209 B CN 114413209B CN 202210315864 A CN202210315864 A CN 202210315864A CN 114413209 B CN114413209 B CN 114413209B
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21S—NON-PORTABLE LIGHTING DEVICES; SYSTEMS THEREOF; VEHICLE LIGHTING DEVICES SPECIALLY ADAPTED FOR VEHICLE EXTERIORS
- F21S8/00—Lighting devices intended for fixed installation
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V23/00—Arrangement of electric circuit elements in or on lighting devices
- F21V23/04—Arrangement of electric circuit elements in or on lighting devices the elements being switches
- F21V23/0442—Arrangement of electric circuit elements in or on lighting devices the elements being switches activated by means of a sensor, e.g. motion or photodetectors
- F21V23/0464—Arrangement of electric circuit elements in or on lighting devices the elements being switches activated by means of a sensor, e.g. motion or photodetectors the sensor sensing the level of ambient illumination, e.g. dawn or dusk sensors
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V5/00—Refractors for light sources
- F21V5/008—Combination of two or more successive refractors along an optical axis
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V5/00—Refractors for light sources
- F21V5/04—Refractors for light sources of lens shape
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Abstract
The invention discloses a monitoring device and a dissociation device, wherein the monitoring device is used for monitoring the dissociation device and comprises: a plurality of light supplement devices and an image recognition device. Each light supplementing device comprises a light emitting part and a light guide part, the light emitting part is arranged on one side of the functional cylinder, and the light guide part is used for guiding light rays emitted by the light emitting part to the position, close to the lower part, of the corresponding functional cylinder in the direction to emit the light rays, so that the light rays are prevented from being shielded by the mounting seat or other components, and the light supplementing effect is better. The image recognition device comprises an image assembly and a controller, the image assembly is used for acquiring detection images in the functional cylinder, the controller is used for controlling the corresponding light supplementing device to emit light when the light intensity of the functional cylinder is smaller than a preset value, so that the automatic switch of the light supplementing device can be realized, the consumption can be saved, and the device is more convenient.
Description
Technical Field
The invention relates to the technical field of monitoring equipment, in particular to a monitoring device and a dissociation device.
Background
Tissue dissociation is an indispensable and important step in cell level detection and is also a basic technique commonly used in biomedical laboratories. When the tissue is dissociated, the dissociation device needs to put dissociation liquid with cell tissue into the dissociation chamber for dissociation. The functional cylinder of the dissociation device needs to be monitored in the dissociation process so as to judge the process of tissue dissociation, and accurate results can be observed only by sufficient illumination of an observation environment. Monitoring devices among the current dissociation device can all be towards the mount pad of function barrel transmission light when carrying out the light filling, but this makes most light blockked by the mount pad, and the light that leads to in the function barrel is still relatively poor.
Disclosure of Invention
The invention mainly aims to provide a monitoring device, and aims to solve the problem that the traditional monitoring device is poor in light supplementing effect when used for supplementing light.
In order to achieve the above object, the present invention provides a monitoring device for monitoring a dissociation device, the monitoring device comprising:
the light supplementing devices are arranged on the mounting seat of the dissociation device and correspond to the functional cylinders of the dissociation device one to one, each light supplementing device comprises a light emitting part and a light guide part, the light emitting part is arranged on one side of the corresponding functional cylinder, and the light guide part is used for guiding light rays emitted by the light emitting part to the position close to the lower part of the corresponding functional cylinder for emission; and (c) a second step of,
the image recognition device comprises an image assembly and a controller which are electrically connected with each other, the image assembly is used for acquiring detection images in each functional barrel, the controller is electrically connected with each light supplementing device, the controller is used for determining the light intensity of the functional barrel according to the received detection images, and the corresponding light supplementing devices are controlled to emit light when the light intensity of the functional barrel is smaller than a preset value.
Optionally, the monitoring device further includes a plurality of annular enclosing plates which are used for being installed in the installation seat in an upward protruding manner and are arranged in one-to-one correspondence with the plurality of functional cylinders, each annular enclosing plate is used for enclosing an opening portion of the corresponding functional cylinder, and the annular enclosing plates are made of a light-transmitting material.
Optionally, each of the annular enclosing plates has a light emitting surface, and the light emitting surface is arranged to be inclined in a direction gradually deviating from the central axis of the functional cylinder from top to bottom, so that light emitted through the light emitting surface is emitted toward the lower portion of the corresponding functional cylinder.
Optionally, the light guide portion includes:
the block-shaped main body is used for being arranged on the mounting seat close to the corresponding functional cylinder, and a mounting groove used for facing the opening of the functional cylinder is formed in the block-shaped main body; and the number of the first and second groups,
the refractive lens group comprises a refractive lens, the refractive lens is movably arranged in the mounting groove in the direction close to and far away from the notch of the mounting groove, so that the refractive lens has a working position located at the notch of the mounting groove in the moving process;
the light-emitting part comprises a light source, the light source is installed on the upper side wall of the installation groove, and the refraction lens is used for refracting light rays emitted by the light source downwards to the lower part of the functional cylinder body in the working position.
Optionally, the light supplement device further includes a rotation block disposed on an upper side wall of the mounting groove and rotatable about an axis in an up-down direction, and a driver for driving the rotation block to rotate, and the light source is disposed below a rotation shaft of the rotation block;
the refraction lens sets up along extending from top to bottom, just the upper end of refraction lens install in the turning block is kept away from one side of light source, in order when the turning block rotates, drive the refraction lens winds the light source rotates.
Optionally, the refractive lens group includes a concave lens, a convex lens and the refractive lens, which are sequentially disposed on the rotating block in a direction from the light source to the opening of the mounting groove.
Optionally, the image component is further configured to acquire a working image inside each functional barrel;
the controller is further used for controlling the image assembly to acquire the working image of each functional cylinder when the light intensity of the functional cylinders is not smaller than a preset value.
Optionally, the monitoring device further includes a prompt component, and the controller is electrically connected to the prompt component to control the prompt component to send a prompt when the working image is not matched with a set image in a preset database.
The invention also provides a dissociation device, comprising:
the device comprises a device main body, a plurality of functional cylinders and a control device, wherein the device main body comprises a mounting seat and a plurality of functional cylinders which are arranged on the mounting seat along the vertical direction, and an opening is formed in the upper end of each functional cylinder;
the cover plate is covered at the openings of the functional cylinders; and (c) a second step of,
according to the monitoring device, the monitoring device is detachably arranged on the mounting seat.
Optionally, the cover plate at least corresponds to one opening of the functional cylinder body and is in transparent arrangement.
In the technical scheme of the invention, the light emitting part emits light, and the light guide part guides the light emitted by the light emitting part to the lower part of the functional cylinder, so that the inside of the functional cylinder is fully illuminated. The light guide part can change the propagation direction of light, so that the light is prevented from being shielded by the mounting seat or other parts, and the light supplementing effect is better. In the scheme, whether the functional cylinder needs light supplement is judged by detecting the image. Specifically, the detection image in the functional cylinder is acquired through the image assembly, the light intensity in the functional cylinder is determined through the controller according to the detection image, so that the light in the functional cylinder is judged to be insufficient, and the light supplementing device is controlled to supplement light correspondingly. So can realize light filling device's automatic switch can practice thrift the consumption, and is also more convenient.
Drawings
In order to more clearly illustrate the embodiments or technical solutions of the present invention, the drawings used in the embodiments or technical solutions of the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
Fig. 1 is a schematic structural diagram of an embodiment of a dissociation apparatus provided in the present invention; (cover plate is not covered)
FIG. 2 is a schematic view of another state of the dissociation apparatus shown in FIG. 1; (cover plate)
Fig. 3 is a schematic structural diagram of a light supplement device in the monitoring device according to an embodiment of the present invention.
The reference numbers indicate:
| reference numerals | Name(s) | Reference numerals | Name (R) |
| 1 | Light supplement device | 11 | Light emitting part |
| 111 | |
12 | |
| 121 | Block-shaped |
122 | |
| 123 | |
1231 | |
| 1232 | Convex |
1233 | |
| 124 | |
125 | |
| 13 | |
1000 | |
| 1100 | |
1110 | |
| 1120 | |
1200 | Cover plate |
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.
It should be noted that, if directional indications (such as up, down, left, right, front, and back … …) are involved in the embodiment of the present invention, the directional indications are only used for explaining the relative position relationship between the components, the motion situation, and the like under a certain posture (as shown in the drawing), and if the certain posture is changed, the directional indications are changed accordingly.
In addition, if there is a description relating to "first", "second", etc. in the embodiments of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In addition, the meaning of "and/or" appearing throughout includes three juxtapositions, exemplified by "A and/or B", including either A or B or both A and B. In addition, technical solutions between the embodiments may be combined with each other, but must be based on the realization of the technical solutions by a person skilled in the art, and when the technical solutions are contradictory to each other or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
When the tissue is dissociated, the dissociation device needs to put the dissociation solution with the cell tissue in the dissociation chamber for dissociation. The functional cylinder of the dissociation device needs to be monitored in the dissociation process so as to judge the process of tissue dissociation, and accurate results can be observed only when the illumination of the observation environment is sufficient. Monitoring devices among the current dissociation device all can be towards the mount pad of function barrel transmission light when carrying out the light filling, but this makes most light blockked by the mount pad, leads to the light in the function barrel still relatively poor.
In view of this, the present invention provides a monitoring device, which aims to solve the problem of poor light compensation effect when the conventional monitoring device performs light compensation. Fig. 1 to fig. 2 are diagrams illustrating an embodiment of a dissociation device provided by the present invention, and fig. 3 is a diagram illustrating an embodiment of a light supplement device in a monitoring device provided by the present invention.
Referring to fig. 1 to 3, a monitoring device for monitoring a dissociation device 1000 according to the present invention includes: a plurality of light supplement devices 1 and an image recognition device. The plurality of light supplement devices 1 are arranged on the mounting seat 1110 of the dissociation device 1000 and correspond to the plurality of functional cylinders 1120 of the dissociation device 1000 one to one, each light supplement device 1 includes a light emitting portion 11 and a light guide portion 12, the light emitting portion 11 is arranged on one side of the corresponding functional cylinder 1120, and the light guide portion 12 is used for guiding the light emitted from the light emitting portion 11 to the lower portion of the corresponding functional cylinder 1120 for emission; the image recognition device comprises an image component and a controller which are electrically connected with each other, the image component is used for acquiring a detection image in each functional cylinder 1120, the controller is electrically connected with each light supplementing device 1, and the controller is used for determining the light intensity of the functional cylinder 1120 according to the received detection image and controlling the corresponding light supplementing device 1 to emit light when the light intensity of the functional cylinder 1120 is smaller than a preset value.
In the present invention, the up-down direction is an extending direction of the functional cylinder 1120, wherein the opening of the functional cylinder 1120 is located at the upper end of the functional cylinder 1120.
In the technical solution of the present invention, the light emitting portion 11 emits light, and the light guide portion 12 guides the light emitted from the light emitting portion 11 to a lower portion of the functional cylinder 1120, so as to sufficiently illuminate the inside of the functional cylinder 1120. The light guide part 12 can change the propagation direction of light, so as to prevent the light from being blocked by the mounting seat 1110 or other components, and thus the light supplement effect is better. In this embodiment, whether the functional cylinder 1120 needs light supplement is determined by detecting an image. Specifically, the detection image in the functional cylinder 1120 is acquired through the image component, and then the light intensity in the functional cylinder 1120 is determined through the controller according to the detection image, so that which light in the functional cylinder 1120 is insufficient is determined, and the corresponding light supplement device 1 is controlled to supplement light. Therefore, the automatic switch of the light supplementing device 1 can be realized, the consumption can be saved, and the operation is more convenient.
Further, referring to fig. 1, the monitoring device further includes a plurality of annular surrounding plates 13 protruding upward from the mounting seat 1110 and disposed in one-to-one correspondence with the plurality of functional cylinders 1120, each of the annular surrounding plates 13 is disposed to surround the opening of the corresponding functional cylinder 1120, and each of the annular surrounding plates 13 is made of a light-transmitting material.
The opening at the upper end of the functional cylinder 1120 is arranged, and the light supplement device 1 is arranged close to the opening of the functional cylinder 1120, and in the dissociation process, foreign matters on the light supplement device 1 or other structures may fall into the functional cylinder 1120 from the opening of the functional cylinder 1120, so that the normal operation of dissociation is affected. Thus, in this embodiment, the monitoring device is further provided with the annular enclosing plate 13, so that the opening of the functional cylinder 1120 can be separated from the light supplement device 1, and the annular enclosing plate 13 is made of a light-transmitting material, which does not affect the light supplement of the light supplement device 1.
Further, each of the annular enclosing plates 13 has a light emitting surface, and the light emitting surface is arranged to be inclined in a direction gradually deviating from the central axis of the functional cylinder 1120 from top to bottom, so that light emitted from the light emitting surface is emitted toward the lower portion of the corresponding functional cylinder 1120.
In this embodiment, the light of the light supplement device 1 passes through the annular surrounding plate 13 and enters the functional cylinder 1120, that is, the annular surrounding plate 13 includes a light incident surface into which the light enters and a light emitting surface from which the light exits. The light emitting surface is gradually deviated from the top to the bottom, the direction of the central axis of the functional cylinder 1120 is inclined, according to the refraction principle of light, light can pass through the annular enclosing plate 13 and is deflected downwards when the light emitting surface emits out, so that the light can face the corresponding part, close to the functional cylinder 1120, of the light emitting surface emits out, the obstruction of the installation seat 1110 to the light is reduced, and the light supplementing effect is better.
Further, referring to fig. 3, the light guide part 12 includes: a block-shaped body 121 and a refractive lens set 123. The block-shaped main body 121 is disposed on the mounting seat 1110 near the corresponding functional cylinder 1120, and a mounting groove 122 for opening toward the functional cylinder 1120 is disposed in the block-shaped main body 121; the refractive lens assembly 123 comprises a refractive lens 1233, and the refractive lens 1233 is movably mounted in the mounting groove 122 in a direction close to and away from the opening of the mounting groove 122, so that during the movement, the refractive lens 1233 has a working position located at the opening of the mounting groove 122; the light emitting portion 11 includes a light source 111, the light source 111 is mounted on an upper sidewall of the mounting groove 122, and the refractive lens 1233 is configured to refract light emitted from the light source 111 downward to a lower portion of the functional cylinder 1120 when in the working position.
In this embodiment, the block-shaped body 121 has the mounting groove 122 formed therein, and the notch of the mounting groove 122 is disposed toward the functional cylinder 1120, so that the light emitted from the light source 111 is emitted toward the functional cylinder 1120. The refraction lens 1233 is in the light filling device 1 can move to the mouth of the mounting groove 122 during operation, and at this time the light emitted from the light source 111 is emitted from the mouth of the mounting groove 122, the light can pass through the refraction lens 1233, so the refraction lens 1233 refracts the light of the light source 111, and the emitting direction of the light is changed. This causes light to be refracted downward to the lower portion of the functional cylinder 1120, making the interior of the functional cylinder 1120 brighter and more suitable for observation.
It should be noted that the light source 111 is disposed in the mounting groove 122, and light emitted from the light source 111 cannot be emitted except for the opening of the mounting groove 122, so that the light only can be emitted toward the functional cylinder 1120, and scattering does not affect observation. The refractive lens 1233 is a conventional optical refractive lens 1233, and the refractive lens 1233 can be selected with a suitable refractive index according to the diameter and depth setting of the functional cylinder 1120.
The light supplement device 1 may not only mount the light source 111 by forming the mounting groove 122 in the block body 121, but also mount the light source 111 by directly forming the mounting groove 122 in the sidewall of the functional cylinder 1120 in the light supplement device 1.
Further, the light supplement device 1 further includes a rotating block 124 disposed on an upper sidewall of the mounting groove 122 and rotatable about an axis in a vertical direction, and a driver 125 for driving the rotating block 124 to rotate, wherein the light source 111 is disposed below a rotating shaft of the rotating block 124; the setting is extended to the refraction lens 1233 from top to bottom, just the upper end of refraction lens 1233 install in the turning block 124 is kept away from one side of light source 111, with when the turning block 124 rotates, drive refraction lens 1233 winds light source 111 rotates.
In this embodiment, the driver 125 can drive the rotating block 124 to rotate, so as to drive the refractive lens 1233 to move, so that the refractive lens 1233 can leave the notch of the mounting groove 122. The driver 125 can switch the use state of the refractive lens 1233 by driving the rotation block 124 to rotate. The driver 125 can drive the rotating block 124 to rotate, so as to change the area of the portion of the refractive lens 1233 blocking the notch of the mounting groove 122, thereby obtaining different refractive effects and meeting more use requirements.
The driver 125 may be a motor or a rotary cylinder, and is not limited in the present invention. The driver 125 may be directly electrically connected to the controller, so that the controller controls the rotation of the rotating block 124.
Further, the refractive lens group 123 includes a concave lens 1231, a convex lens 1232 and the refractive lens 1233 sequentially disposed on the rotating block 124 in a direction from the light source 111 to the opening of the mounting groove 122.
In this embodiment, the light of the light source 111 is firstly diverged to a certain degree by the concave lens 1231, and then converged by the convex lens 1232, and the astigmatism of the light source 111 can be adjusted to be parallel light by calculating the curvatures of the concave lens 1231 and the convex lens 1232, that is, the concave lens 1231 and the convex lens 1232 form a collimating lens group in this embodiment. The parallel light passes through this moment again refractive lens 1233, the parallel light that refractive lens 1233 refracted out can be even jets into in the function barrel 1120, and this can let the light of function barrel 1120 is more even, can not appear the light gathering and take place dazzling, perhaps influences the observation effect.
Further, the image component is also used for acquiring a working image in each functional cylinder 1120; the controller is further configured to control the image component to obtain the working image of each functional cylinder 1120 when the light intensity of the functional cylinder 1120 is not less than a preset value.
In this embodiment, the specific content included in the working image is not limited, and the working image may be a color of the dissociation liquid, may be a light intensity in the functional cylinder 1120, may also be a turbidity degree of the dissociation liquid, and the like. The image component can be an industrial camera or a light sensor, and the specific structure can be set according to the required working image.
When the light in the functional cylinder 1120 is poor, the controller controls the light supplement device 1 to work, so that the light intensity in the functional cylinder 1120 is greater than a preset value, and the image assembly acquires the accurate working image. And after receiving the working image, the controller can match the working image with a preset database so as to judge the current dissociation condition and ensure the normal operation of cell dissociation.
Further, the monitoring device further comprises a prompt component, and the controller is electrically connected with the prompt component to control the prompt component to send out a prompt when the working image is not matched with the set image in the preset database.
In this embodiment, the prompt component may be implemented in various ways, such as a display, a broadcast or alarm device, and the like, and may be specifically set according to a use requirement, which is not limited in this embodiment. The controller is receiving behind the working image, can be according to the working image matches with predetermineeing the database to judge current dissociation situation, and works as the working image explains that the dissociation appears unusually when not matching with the settlement image of predetermineeing in the database, consequently passes through the suggestion subassembly sends the suggestion, reminds the user in time to deal with.
Not only when the working image is not matched with the set image in the preset database, when the image assembly acquires the image when the dissociation is completed or a certain stage of dissociation is completed, the controller can also control the prompt assembly to send out a prompt, and certainly, the prompt assembly can be respectively carried out through different prompt modes.
Referring to fig. 1 to fig. 2, the present invention further provides a dissociation apparatus 1000, including: a device body 1100, a cover plate 1200 and a monitoring device as described in any of the above embodiments. The device main body 1100 includes a mounting seat 1110 and a plurality of functional cylinders 1120 provided in the mounting seat 1110 in the up-down direction, and an upper end of each functional cylinder 1120 is open; the cover plate 1200 covers the openings of the functional cylinders 1120; the monitoring device is detachably mounted to the mounting seat 1110.
In the technical solution of the present invention, the device body 1100 of the dissociation device 1000 is generally a disposable product, so that the monitoring device is detachably mounted on the mounting seat 1110, thereby allowing the monitoring device to be repeatedly used and reducing the use cost.
In this embodiment, the cover plate 1200 is disposed in a transparent manner at least corresponding to an opening of the functional cylinder 1120. The user can directly observe the dissociation condition in the functional cylinder 1120 through the cover plate 1200, but not necessarily through the monitoring device. So when monitoring devices goes wrong, the user can also directly observe dissociation device 1000 to guarantee the normal clear of dissociation.
The specific working process of the dissociation device 1000 is as follows:
tissue preparation: adding 1 XPBS solution into each groove in the base, and cleaning the samples stored in the tissue storage solution; adding a proper amount of cleaned samples into the dissociation liquid, and cutting the tissues into tissue blocks with proper sizes by using surgical scissors.
Placing the dissociation solution with tissue in the dissociation tank, and acting at 37 deg.C for 15-40min, wherein the dissociation tank needs to break tissue in forward direction and incubate tissue in reverse direction for the same time every 5min, and is optimized to form homogenate solution after tissue digestion;
pumping the homogenate solution in the dissociation tank into the liquid forming tank, and enabling the homogenate solution to pass through the filter screen, wherein the filter screen is a sterile cell screen of 40-70 mu m;
adding an equal volume of stop solution into the filtrate to complete dissociation termination;
adding an equal volume of erythrocyte lysate into the cell suspension, reversing, uniformly mixing, and incubating at room temperature for 2-7 min; stopping the erythrocyte lysis by an equal volume of 1640 solution;
mixing by inversion, and standing at 4 deg.C for 5min at 300 g; removing the supernatant; obtaining single cell sediment, and obtaining single cell suspension after heavy suspension.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification and the drawings, or any other related technical fields, which are directly or indirectly applied to the present invention, are included in the scope of the present invention.
Claims (10)
1. A monitoring device for monitoring a dissociation device, the monitoring device comprising:
the light supplementing devices are arranged on the mounting seat of the dissociation device and correspond to the functional cylinders of the dissociation device one to one, each light supplementing device comprises a light emitting part and a light guide part, the light emitting part is arranged on one side of the corresponding functional cylinder, and the light guide part is used for guiding light rays emitted by the light emitting part to the position close to the lower part of the corresponding functional cylinder for emission; and (c) a second step of,
the image recognition device comprises image assemblies and a controller which are electrically connected with each other, the image assemblies are used for acquiring detection images in the functional cylinders, the controller is electrically connected with the light supplementing devices, the controller is used for determining the light intensity of the functional cylinders according to the received detection images, and the corresponding light supplementing devices are controlled to emit light when the light intensity of the functional cylinders is smaller than a preset value.
2. The monitoring device according to claim 1, further comprising a plurality of annular enclosing plates, which are arranged to protrude upward from the mounting base and correspond to the plurality of functional cylinders one by one, wherein each of the annular enclosing plates is arranged to enclose the mouth of the corresponding functional cylinder, and the annular enclosing plates are made of a light-transmitting material.
3. The apparatus according to claim 2, wherein each of the annular surrounding plates has a light-emitting surface, the light-emitting surface is disposed to be inclined in a direction gradually deviating from the central axis of the functional cylinder from top to bottom, so that light emitted from the light-emitting surface is emitted toward a lower portion of the corresponding functional cylinder.
4. The monitoring device of claim 1, wherein the light directing portion comprises:
the block-shaped main body is used for being arranged on the mounting seat close to the corresponding functional cylinder, and a mounting groove used for facing the opening of the functional cylinder is formed in the block-shaped main body; and the number of the first and second groups,
the refraction lens group comprises refraction lenses, the refraction lenses are movably arranged in the mounting groove in the direction close to and far away from the groove opening of the mounting groove, so that the refraction lenses have working positions positioned at the groove opening of the mounting groove in the moving process;
the light-emitting part comprises a light source, the light source is installed on the upper side wall of the installation groove, and the refraction lens is used for refracting light rays emitted by the light source downwards to the lower part of the functional cylinder body in the working position.
5. The monitoring device according to claim 4, wherein the light supplement device further comprises a rotation block rotatably disposed on an upper side wall of the mounting groove about an axis in a vertical direction, and a driver for driving the rotation block to rotate, wherein the light source is disposed below a rotation shaft of the rotation block;
the refraction lens sets up along extending from top to bottom, just the upper end of refraction lens install in the turning block is kept away from one side of light source, in order when the turning block rotates, drive the refraction lens winds the light source rotates.
6. The apparatus of claim 5, wherein the refractive lens assembly comprises a concave lens, a convex lens and the refractive lens disposed in the turning block in sequence in a direction from the light source to the opening of the mounting groove.
7. The monitoring device of claim 1, wherein the image component is further configured to acquire a working image within each of the functional cartridges;
the controller is further used for controlling the image assembly to acquire the working image of each functional cylinder when the light intensity of the functional cylinders is not smaller than a preset value.
8. The monitoring device of claim 7, further comprising a prompt component, wherein the controller is electrically connected to the prompt component to control the prompt component to send a prompt when the working image is sensed not to match the setting image in the preset database.
9. A dissociation device, comprising:
the device comprises a device main body, a plurality of functional cylinders and a control device, wherein the device main body comprises a mounting seat and a plurality of functional cylinders which are arranged on the mounting seat along the vertical direction, and an opening is formed in the upper end of each functional cylinder;
the cover plate is covered at the openings of the functional cylinders; and (c) a second step of,
the monitoring device of any one of claims 1 to 8, the monitoring device being removably mounted to the mounting.
10. The dissociation device of claim 9, wherein the cover plate is disposed in a transparent manner corresponding to the opening of the at least one functional cylinder.
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| US20190024033A1 (en) * | 2017-07-19 | 2019-01-24 | Cellanyx Diagnostics, Llc | Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells |
| WO2020157077A2 (en) * | 2019-01-28 | 2020-08-06 | Cellink Ab | A compact fluorescence microscope and a cell monitoring system |
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