CN114405302B - Rotary microfluidic device and method for controllably preparing monodisperse emulsion - Google Patents
Rotary microfluidic device and method for controllably preparing monodisperse emulsion Download PDFInfo
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Abstract
The invention provides a rotary microfluidic device for controllably preparing monodisperse emulsion, which comprises a rotary platform, an injection tube, a collection container and an injection pump, wherein the injection tube is arranged on the rotary platform; the rotary platform comprises a motor and a horizontally arranged rotary table, and the motor drives the rotary table to rotate around the circle center of the rotary table at a constant speed; the collecting container is horizontally fixed on the rotary table, and the circle center of the collecting container is superposed with that of the rotary table; the inlet of the injection tube is communicated with the injection pump, the outlet of the injection tube is positioned outside the circle center of the collecting container, and the outlet of the injection tube is positioned below the liquid level of the fluid contained in the collecting container. The invention also provides a controllable preparation method of the monodisperse emulsion based on the device. The invention can make the micro-fluidic device for preparing the monodisperse emulsion and the construction thereof simpler, and the preparation and the yield amplification of the monodisperse emulsion are more convenient and efficient.
Description
Technical Field
The invention belongs to the field of emulsion preparation, and relates to a microfluidic device and a method for preparing monodisperse emulsion.
Background
The emulsion has important function in various fields such as chemical industry, medicine, food, cosmetics and the like. Microfluidic technology, which has emerged in recent years, exhibits unique advantages in the controlled preparation of emulsion droplets because of the precise manipulation of fluid flow and dispersion in microchannels. The micro-flow control emulsion technology generally depends on a micro-flow control device with micro-channels with complex geometric structures such as a coaxial co-flow type, a flow focusing type, a cross-flow type and the like, and the construction of the complex micro-channel structure usually requires a precise and complex micro-manufacturing process and a surface modification technology. Meanwhile, in the process of microfluidic milk production, the flow control of a dispersed phase fluid and a continuous phase fluid in different microchannels and the shearing process of the two-phase fluid at the intersection of the microchannels are usually involved, which often requires a professional skilled person to perform precise and skilled fluid control to prevent the phenomena of dispersed phase adherence, continuous phase backflow and the like from occurring to hinder the formation of emulsion droplets, so that the flow control of each liquid phase has high requirements. In addition, when the construction of multiple microchannels is involved to amplify the emulsion production yield, the construction of multiple microchannels puts higher demands on the microfabrication process and surface modification technology of the existing microfluidic devices, and the manipulation of two-phase fluid in the complex multiple microchannels.
In order to simplify the technology of controlling the emulsion in a microfluidic manner, although researchers develop microfluidic devices based on structures such as centrifuges and rotating conical rotors, the problems of complex device structures and construction processes still exist, and the yield amplification of the emulsion preparation is difficult to effectively realize due to the device structures and the fluid control modes. The above circumstances limit the further development and application of microfluidic emulsion preparation technology. Therefore, developing simpler and more efficient microfluidic devices and methods for controlled preparation of emulsions and production scale-up is of great scientific interest and application.
Disclosure of Invention
Aiming at the problems of complex device structure, complex construction process, high requirement on fluid control, difficulty in effective amplification and the like in the existing microflow control emulsion technology, the invention provides a rotary type microflow control device for controllably preparing monodisperse emulsion, and provides a controllable preparation method of monodisperse emulsion based on the device, so that the microflow control device for preparing monodisperse emulsion is simpler to construct, and the preparation and the yield amplification of the monodisperse emulsion are more convenient and efficient.
In order to realize the purpose of the invention, the technical scheme adopted by the invention is as follows:
a rotary microfluidic device for controllably preparing monodisperse emulsion comprises a rotary platform, an injection tube, a collection container and an injection pump;
the rotary platform comprises a motor and a horizontally arranged rotary table, and the motor drives the rotary table to rotate around the circle center of the rotary table at a constant speed; the collecting container is horizontally fixed on the rotary table, and the circle center of the collecting container is superposed with that of the rotary table; the inlet of the injection tube is communicated with the injection pump, the outlet of the injection tube is positioned outside the circle center of the collecting container, and the outlet of the injection tube is positioned below the liquid level of the fluid contained in the collecting container.
Further, in order to realize the amplification of the output of the monodisperse emulsion, in the above technical scheme of the rotary microfluidic device for controllably preparing the monodisperse emulsion, the device further comprises a fluid distribution plate for fluid distribution, the fluid distribution plate is provided with a fluid inlet and a plurality of fluid outlets, the distances between the fluid inlet and the fluid outlets are equal, the fluid inlet and the fluid outlets are communicated by a fluid channel, the fluid distribution plate is horizontally arranged, the fluid inlet is communicated with an injection pump, the fluid outlets are respectively communicated with the inlets of the injection pipes, and the distances between the outlets of the injection pipes and the circle center of the collection container are equal. Further, the fluid outlets of the fluid distribution plate are evenly distributed around the fluid inlet.
For example, in the above technical solution of the rotary microfluidic device for controllably preparing a monodisperse emulsion, the fluid distribution plate may be circular, the fluid inlets are disposed at the center of the fluid distribution plate, and the fluid outlets are uniformly distributed on the same circle concentric with the fluid distribution plate around the fluid inlets.
In the above technical solution of the rotary microfluidic device for controllably preparing monodisperse emulsion, the injection tube may be made of metal, polymer, or glass, and the inner diameter of the outlet of the injection tube is preferably 10-1000 μm.
In the technical scheme of the rotary microfluidic device for controllably preparing the monodisperse emulsion, the distance between the outlet of the injection tube and the circle center of the collecting container is at least 1cm.
In the technical scheme of the rotary microfluidic device for controllably preparing the monodisperse emulsion, the cross section of the collecting container is circular.
The invention also provides a controllable preparation method of the monodisperse emulsion based on the rotary microfluidic device, which comprises the following steps:
the continuous phase fluid is contained in a collecting container, and the motor drives a turntable to drive the collecting container and the continuous phase fluid therein to rotate around the center of a circle of the collecting container at a constant speed; then, injecting the dispersed phase fluid into the continuous phase fluid in the collecting container through an injection pipe by using an injection pump, namely, obtaining monodisperse emulsion droplets through liquid phase shearing, or injecting the dispersed phase fluid into each injection pipe through a fluid inlet of a fluid distribution plate by using the injection pump, distributing the dispersed phase fluid into each injection pipe, and then flowing into the continuous phase fluid in the collecting container through the injection pipes, namely obtaining the monodisperse emulsion droplets through liquid phase shearing;
the rotation speed of the motor and the flow rate of the dispersed phase fluid are controlled in the preparation process, so that the continuous phase fluid can shear and separate the dispersed phase fluid from the outlet of the injection tube through the rotary shearing to form emulsion droplets.
In the technical scheme of the controllable preparation method of the monodisperse emulsion, the disperse phase fluid and the continuous phase fluid are two-phase fluids which are not mutually soluble or slightly soluble.
Further, taking the aqueous phase solution as a dispersed phase fluid and the oil phase solution as a continuous phase fluid; or, the oil phase solution is used as the dispersed phase fluid, and the water phase solution is used as the continuous phase fluid; or, taking liquid metal as dispersed phase fluid and taking water phase solution or oil phase solution as continuous phase fluid; or two water phase solutions which are not soluble or slightly soluble with each other are respectively used as a dispersed phase fluid and a continuous phase fluid; alternatively, two oil phase solutions which are insoluble or slightly soluble with each other are used as the dispersed phase fluid and the continuous phase fluid, respectively.
Further, the continuous phase fluid may contain an interface stabilizing agent, for example, the interface stabilizing agent includes a surfactant or nanoparticles for stabilizing the interface, and the surfactant is dissolved in the continuous phase fluid, and the nanoparticles for stabilizing the interface are uniformly dispersed in the continuous phase fluid.
Further, the aqueous phase solution or/and the oil phase solution may contain at least one of a functional polymer, a monomer, a thickener, a salt, and a functional nanoparticle, which is soluble in the aqueous phase solution or the oil phase solution. For example, when the dispersed phase fluid contains functional polymer or monomer, the droplets of the monodisperse emulsion prepared by the above method can be solidified to obtain functionalized monodisperse polymer microspheres; when the dispersed phase fluid contains functional nano-particles and functional polymers or monomers, the droplets of the monodisperse emulsion prepared by the method are solidified to obtain the functionalized monodisperse polymer microspheres containing nano-particles.
In practical applications, the formulations of the dispersed phase fluid and the continuous phase fluid are determined according to specific application requirements, and the formulations of the dispersed phase fluid and the continuous phase fluid can be determined by referring to the prior art.
In practical applications, under the condition that the structure of the rotary microfluidic device for controllably preparing the monodisperse emulsion, the formula of the disperse phase fluid and the continuous phase fluid are determined, the rotating speed of the motor and the flow rate of the disperse phase fluid can be determined through experiments, so that the continuous phase fluid can be ensured to be sheared and separated from the outlet of the injection tube to form liquid drops through rotary shearing.
In the technical scheme of the controllable preparation method of the monodisperse emulsion, the diameter of the prepared monodisperse emulsion liquid drop can be flexibly adjusted by adjusting the rotating speed of the collection container rotating around the circle center of the collection container at a constant speed, the distance between the outlet of the injection tube and the circle center of the collection container, the inner diameter of the outlet of the injection tube, the flow of the disperse phase fluid and the like. Generally, the diameter of the monodisperse emulsion droplets produced by the above process is any value between 20 and 1000. Mu.m.
Compared with the prior art, the technical scheme provided by the invention has the following beneficial technical effects:
1. the invention provides a rotary microfluidic device for controllably preparing monodisperse emulsion, which has a simple structure, and does not need complex microfabrication process and surface modification technology for device construction; meanwhile, the number of injection pipes can be increased by introducing the fluid distribution plate, so that the amplification of the number of micro-channels is conveniently realized, and the method has unique advantages in the aspect of controllable preparation of the monodisperse emulsion.
2. The controllable preparation method of the monodisperse emulsion based on the rotary microfluidic device skillfully utilizes the process that the disperse phase fluid is sheared by the continuous phase fluid in the open collecting container, effectively avoids the problems of adherence of the disperse phase, backflow of the continuous phase and the like, reduces the requirements on the professional technology of operators, and ensures that the fluid is more convenient and flexible to control. Meanwhile, the method has simple and flexible device structure and fluid control mode, can conveniently and effectively amplify the emulsion preparation yield under the condition of only adding a fluid distribution plate and an injection pipe on the premise of the existing rotary type shearing emulsion preparation mode, and has great application value.
3. The controllable preparation method of the monodisperse emulsion based on the rotary microfluidic device provided by the invention can be suitable for various liquid phase systems, can be used for the liquid phase systems applied in the existing microfluidic technology, and can also be used for preparing emulsion droplets (such as liquid metal) with the characteristics of high surface tension, large density and the like, so that the controllable preparation method of the monodisperse emulsion based on the rotary microfluidic device can be widely used for preparing various liquid phase emulsions.
Drawings
FIG. 1 is a schematic diagram of a rotary microfluidic device for controlled preparation of monodisperse emulsions, in which 1 is a rotary platform, 2 is an injection tube, 3 is a collection vessel, and 4 is an injection pump.
Fig. 2 is a schematic diagram of the structure of a fluid distribution plate of a rotary microfluidic device, in which 6 is a fluid distribution plate, 6-1 is a fluid inlet, and 6-2 is a fluid channel.
Figure 3 is a photograph of six syringes attached to a fluid distribution plate of a rotating microfluidic device.
FIGS. 4 a) to d) are respectively optical microscope pictures of the W/O emulsion prepared in example 3, the diameter distribution of the emulsion droplets, and the diameter of the emulsion droplets as a function of the linear velocity of the continuous phase fluid and the flow rate of the dispersed phase.
FIGS. 5 a) to d) are respectively optical microscope pictures of the O/W emulsion prepared in example 4, the diameter distribution of the emulsion droplets, and the diameter of the emulsion droplets as a function of the linear velocity of the continuous phase fluid and the flow rate of the dispersed phase.
Fig. 6 a) to b) are respectively an optical microscope photograph of the liquid metallic gallium droplet prepared in example 5 and a diameter distribution diagram of the liquid metallic gallium droplet.
Fig. 7 a) to b) are respectively an optical microscope photograph of the liquid metallic gallium droplet prepared in example 6 and a diameter distribution diagram of the liquid metallic gallium droplet.
FIGS. 8 a) to b) are graphs showing the influence of the flow rate of the dispersed phase on the droplet diameter and the coefficient of variation of the emulsion in example 7, respectively, and b) is an optical microscope photograph of the emulsion obtained when the flow rate of the dispersed phase is 36 mL/h.
Detailed Description
The present invention provides a rotary microfluidic device for controllably preparing a monodisperse emulsion, and a method for controllably preparing a monodisperse emulsion based on the rotary microfluidic device, which are described below by way of examples and with reference to the accompanying drawings. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and those skilled in the art can make certain insubstantial modifications and adaptations of the present invention based on the above disclosure and still fall within the scope of the present invention.
The water-soluble surfactant used in each of the following examples was Pluronic F-127, which was a segmented polyether F127 and was an addition polymer of polypropylene glycol and ethylene oxide, pluronic F-127, which was a trade name and was obtained from Sigma; the oil-soluble surfactant is polyisobutenyl succinimide (T154).
Example 1
In this embodiment, a schematic structural diagram of a rotary microfluidic device for controllably preparing a monodisperse emulsion is provided, and the rotary microfluidic device is shown in fig. 1 and includes a rotary platform 1, an injection tube 2, a collection container 3, and an injection pump 4.
The rotary platform 1 comprises a motor and a horizontally arranged rotary table, the rotary table is circular, and the rotary table is driven by the motor to rotate around the circle center of the rotary table at a constant speed; the cross section of the collecting container 3 is circular, in particular to a glass culture dish with the diameter of 20cm, the collecting container is horizontally fixed on the turntable, namely the circular bottom surface of the culture dish is horizontally fixed on the turntable, and the circle center of the culture dish is superposed with the circle center of the turntable; the motor drives the turntable to rotate at a constant speed, and further drives the collecting container on the turntable and the continuous phase fluid in the collecting container to rotate at a constant speed around the circle center of the collecting container. The injection pipe 2 is arranged vertical to the horizontal plane, the inlet of the injection pipe is communicated with the liquid outlet of the injection pump, the distance between the outlet of the injection pipe and the circle center of the collecting container is 3-6 cm, and the outlet of the injection pipe is positioned below the liquid level of the continuous phase fluid contained in the collecting container.
In this embodiment, the injection tube 2 is a stainless steel needle or a quartz glass capillary tube with a conical tail, and the injection tubes used in embodiments 3 to 5 and 7 are 34G stainless steel needles, with an inner diameter of 110 μm and an outer diameter of 244 μm; the injection tube used in example 6 was a quartz glass capillary having an inner diameter of 500 μm and an outer diameter of 960 μm, the end portion of which was drawn by a pin drawing machine and processed into a conical flat mouth having an inner diameter of about 30 μm and an outer diameter of about 60 μm.
Example 2
In this embodiment, a rotary microfluidic device for controllably preparing a monodisperse emulsion is provided, which includes a rotary platform 1, an injection tube 2, a collection container 3, an injection pump 4, and a fluid distribution plate 6.
The rotary platform 1 comprises a motor and a horizontally arranged rotary table, the rotary table is circular, and the rotary table is driven by the motor to rotate around the circle center of the rotary table at a constant speed; the cross section of the collecting container 3 is circular, in particular to a glass culture dish with the diameter of 20cm, the collecting container is horizontally fixed on the turntable, namely the circular bottom surface of the culture dish is horizontally fixed on the turntable, and the circle center of the culture dish is superposed with the circle center of the turntable; the motor drives the turntable to rotate at a constant speed, and further drives the collecting container on the turntable and the continuous phase fluid in the collecting container to rotate at a constant speed around the circle center of the collecting container.
The structural schematic diagram of the fluid distribution plate is shown in fig. 2, the fluid distribution plate is circular, the fluid distribution plate is provided with a fluid inlet 6-1 and six fluid outlets, the fluid inlet is communicated with the fluid outlets through a fluid channel 6-2, the fluid inlet is located at the circle center of the fluid distribution plate, the fluid outlets are uniformly distributed around the fluid inlet 6-1, the distances between the fluid inlet and the fluid outlets are equal, and thus the lengths of the fluid channels are equal; the fluid distribution plate is horizontally arranged, a fluid inlet is communicated with a liquid outlet of the injection pump through a pipe fitting, each fluid outlet is respectively communicated with an inlet of each injection tube 2, each injection tube is arranged vertical to the horizontal plane, the distance between the outlet of each injection tube and the circle center of the collecting container is equal and 3cm, and the outlet of each injection tube is positioned below the liquid level of the continuous phase fluid contained in the collecting container.
In this example, six injection tubes were 34G stainless steel needles having an inner diameter of 110 μm and an outer diameter of 244 μm, and the photographs of the injection tubes after being attached to the fluid distribution plate are shown in FIG. 3.
Example 3
In this example, a controllable preparation method of a monodisperse water-in-oil (W/O) emulsion is described as an example, and includes the following steps:
(1) Formulating dispersed and continuous phase fluids
Deionized water is used as a dispersed phase fluid; dissolving T154 in soybean oil to obtain continuous phase fluid, wherein the concentration of T154 in the continuous phase fluid is 0.04g/mL.
(2) Preparation of a monodisperse W/O emulsion
The preparation was carried out using the rotary microfluidic device described in example 1, the continuous phase fluid was contained in a petri dish, the outlet of the injection tube was controlled to be located below the liquid level of the continuous phase fluid contained in the petri dish, and the distance between the outlet of the injection tube and the center of the circle of the petri dish was 4cm. The motor drives the turntable to drive the culture dish to rotate around the center of the culture dish at a constant speed of 0.9r/s, the center of the culture dish is superposed with the center of the horizontally arranged turntable, and at the moment, the continuous phase fluid in the culture dish is driven to rotate around the center of the culture dish at a constant speed; the linear velocity of the continuous phase fluid at the outlet of the injection pipe is 226.08mm/s, and the dispersed phase fluid is injected into the continuous phase fluid from the injection pipe by the injection pump at the flow rate of 1mL/h, so that the monodisperse W/O emulsion is prepared.
FIGS. 4 a) to b) are respectively an optical microscope photograph and a diameter distribution diagram of emulsion droplets of the W/O emulsion prepared in this example, and it can be seen that the droplet size of the W/O emulsion prepared in this example is uniform, the diameter distribution thereof is normal, and the average diameter of the emulsion droplets is 191.81 μm.
The emulsion droplet diameter can be controlled by controlling the linear velocity of the continuous phase fluid (i.e. the rotation speed of the culture dish) and the flow rate of the dispersed phase fluid at the outlet of the injection tube, and the experiment is as follows:
according to the operation of step (2), under the condition of the dispersed phase fluid flow rate of 1mL/h, the linear velocity of the continuous phase fluid at the outlet of the injection tube is respectively controlled to be 25.12, 75.36, 100.48, 125.6, 150.72, 175.84 and 226.08mm/s, so that the diameter of the monodisperse W/O emulsion prepared is shown in FIG. 4 c).
According to the operation of step (2), under the condition that the linear velocity of the continuous phase fluid at the outlet of the injection tube is 75.36mm/s, the flow rates of the dispersed phase fluids are respectively controlled to be 0.5, 1, 3, 5, 8 and 10mL/h, so that the diameters of the monodisperse W/O emulsions prepared by the method are shown in FIG. 4 d), and it can be seen that the diameters of W/O emulsion droplets are gradually increased along with the increase of the flow rate of the dispersed phase fluid under the condition that the linear velocity of the continuous phase fluid is fixed.
Example 4
In this example, taking the preparation process of a monodisperse oil-in-water (O/W) emulsion as an example, the controllable preparation method of a monodisperse emulsion provided by the present invention comprises the following steps:
(1) Formulating dispersed and continuous phase fluids
Soybean oil is used as a dispersed phase fluid; dissolving Pluronic F-127 and sodium carboxymethylcellulose in deionized water to obtain a continuous phase fluid, wherein the mass ratio of the deionized water to the Pluronic F-127 to the sodium carboxymethylcellulose in the continuous phase fluid is 1.
(2) Preparation of a monodisperse O/W emulsion
The preparation was carried out using the rotary microfluidic device described in example 1, the continuous phase fluid was contained in a petri dish, the outlet of the injection tube was controlled to be located below the liquid level of the continuous phase fluid contained in the petri dish, and the distance between the outlet of the injection tube and the center of the circle of the petri dish was 4cm. The motor drives the turntable to drive the culture dish to rotate around the center of the culture dish at a constant speed of 0.7r/s, the center of the culture dish is superposed with the center of the horizontally arranged turntable, at the moment, the continuous phase fluid in the culture dish is driven to rotate around the center of the culture dish at a constant speed, the linear speed of the continuous phase fluid at the outlet of the injection pipe is 175.84mm/s, and the dispersed phase fluid is injected into the continuous phase fluid from the injection pipe at a flow rate of 1mL/h through the injection pump, so that the monodisperse O/W emulsion is obtained.
FIGS. 5 a) to b) are respectively an optical microscope photograph and a diameter distribution diagram of emulsion droplets of the O/W emulsion prepared in this example, and it can be seen that the droplets of the O/W emulsion prepared in this example have uniform size, normal diameter distribution, and mean diameter of 231.18 μm.
The emulsion droplet diameter can be controlled by controlling the linear velocity of the continuous phase fluid (i.e. the rotation speed of the culture dish) and the flow rate of the dispersed phase fluid at the outlet of the injection tube, and the experiment is as follows:
according to the operation of step (2), under the condition of the dispersed phase fluid flow rate of 1mL/h, the linear velocity of the continuous phase fluid at the outlet of the injection tube is respectively controlled to be 25.12, 50.24, 62.8, 75.36, 94.2, 125.6, 175.84, 188.4 and 226.08mm/s, so that the diameter of the monodisperse O/W emulsion prepared by the method is shown in FIG. 5 c).
According to the operation of step (2), the flow rates of the dispersed phase fluids were controlled to be 1, 1.5, 2, 2.5 and 3mL/h under the condition that the linear velocity of the continuous phase fluid at the outlet of the injection tube was 56.52mm/s, and the diameters of the monodisperse O/W emulsions thus prepared were as shown in fig. 5 d).
In example 3, the density of the dispersed phase fluid was greater than the density of the continuous phase fluid, while in example 4, the dispersed phase fluid was less than the density of the continuous phase fluid, in both cases, a monodispersed emulsion was successfully prepared, indicating that the method of the present invention is applicable to systems where the densities of the dispersed phase fluid and the continuous phase fluid differ.
Example 5
In this embodiment, a controllable preparation method of a monodisperse emulsion according to the present invention is described by taking a preparation process of a monodisperse liquid metal gallium droplet as an example, and includes the following steps:
(1) Formulating dispersed and continuous phase fluids
Taking liquid metal gallium as a dispersed phase fluid; pluronic F-127 and sodium carboxymethyl cellulose are dissolved in deionized water to obtain a continuous phase fluid, and the mass ratio of the deionized water to the Pluronic F-127 to the sodium carboxymethyl cellulose in the continuous phase fluid is 1.
(2) Preparation of monodisperse liquid metal gallium drops
The rotary microfluidic device described in example 1 was used to prepare a continuous phase fluid in a petri dish, and the outlet of the injection tube was controlled to be located below the level of the fluid contained in the petri dish, with a distance of 5cm between the outlet of the injection tube and the center of the petri dish. The motor drives the turntable to drive the culture dish to rotate around the center of the culture dish at a constant speed of 0.5r/s, the center of the culture dish is superposed with the center of the horizontally arranged turntable, at the moment, the continuous phase fluid in the culture dish is driven to rotate around the center of the culture dish at a constant speed, the linear speed of the continuous phase fluid at the outlet of the injection pipe is 157mm/s, and the dispersed phase fluid is injected into the continuous phase fluid from the injection pipe at a flow rate of 4.5mL/h through the injection pump, so that the monodisperse liquid metal gallium liquid drop is obtained.
Fig. 6 a) to b) are an optical microscope photograph and a diameter distribution diagram of the liquid metal gallium droplets prepared in this example, respectively, and it can be seen that the liquid metal gallium droplets prepared in this example have uniform size, normal diameter distribution, and average diameter of the emulsion droplets of 285.09 μm.
Example 6
In this embodiment, a controllable preparation method of a monodisperse emulsion provided by the present invention is described by taking a preparation process of a monodisperse liquid metal gallium droplet as an example, and the steps are as follows:
(1) Formulating dispersed and continuous phase fluids
Taking liquid metal gallium as dispersed phase fluid; pluronic F-127 and sodium carboxymethyl cellulose are dissolved in deionized water to obtain a continuous phase fluid, and the mass ratio of the deionized water to the Pluronic F-127 to the sodium carboxymethyl cellulose in the continuous phase fluid is 1.
(2) Preparation of monodisperse liquid metal gallium emulsion
The rotary microfluidic device described in example 1 was used to prepare a continuous phase fluid in a petri dish, the outlet of the injection tube was controlled to be below the level of the fluid in the petri dish, and the distance between the outlet of the injection tube and the center of the petri dish was 6cm. The motor drives the turntable to drive the culture dish to rotate around the center of the culture dish at a constant speed of 0.8r/s, the center of the culture dish is superposed with the center of the horizontally arranged turntable, at the moment, the continuous phase in the culture dish is driven to rotate around the center of the culture dish at a constant speed, the linear speed of the continuous phase fluid at the outlet of the injection pipe is 301.44mm/s, and the dispersed phase fluid is injected into the continuous phase fluid from the injection pipe at a flow rate of 4.5mL/h through the injection pump, so that the monodisperse liquid metal gallium liquid drop is obtained.
Fig. 7 a) to b) are respectively an optical microscope picture of the liquid metal gallium droplet prepared in this example and a diameter distribution diagram of the droplet, and it can be seen that the liquid metal gallium droplet prepared in this example has a uniform size, a normal distribution of the diameter distribution, and an average diameter of 81.84 μm of the emulsion droplet.
Example 7
In this embodiment, a controllable preparation method of a monodisperse emulsion provided by the present invention is described by taking an amplified preparation process of a monodisperse W/O emulsion as an example, and the steps are as follows:
(1) Formulating dispersed and continuous phase fluids
Deionized water is used as a dispersed phase fluid; dissolving T154 in soybean oil to obtain continuous phase fluid, wherein the concentration of T154 in the continuous phase fluid is 0.04g/mL.
(2) Preparation of a monodisperse W/O emulsion
The preparation was carried out using the rotary microfluidic device described in example 2, the continuous phase fluid was contained in a petri dish, the outlets of the injection tubes were controlled to be located below the liquid level of the continuous phase fluid contained in the petri dish, and the distance between the outlet of each injection tube and the center of the petri dish was 3cm. The motor drives the turntable to drive the culture dish to rotate around the axis of the culture dish at a constant speed of 0.6r/s, the centers of the turntables horizontally arranged at the center of the culture dish coincide, at the moment, the continuous phase in the culture dish is driven to rotate around the center of the culture dish, the linear velocity of the continuous phase fluid at the outlet of each injection tube is 113.04mm/s, the dispersed phase fluid is injected into the fluid distribution plate from the fluid inlet of the fluid distribution plate at a flow rate of 18mL/h through the injection pump, and the dispersed phase fluid passes through the fluid channel on the fluid distribution plate and then is injected into the continuous phase fluid through the injection tube, so that the monodisperse W/O emulsion is prepared.
According to the operation of step (2), under the condition that the linear velocity of the continuous phase fluid at the outlet of each injection tube is 113.04mm/s, the flow rates of the dispersed phase fluids are controlled to be 24, 30, 36, 42, 48 and 54mL/h, and the diameters of the monodisperse W/O emulsions prepared therefrom are shown in fig. 8 a). As can be seen from fig. 8 (b), the W/O emulsion droplets prepared in this example all have a coefficient of variation of less than 5% in diameter, indicating that the prepared W/O emulsion droplets still have good monodispersity under the condition of production scale-up. As can be seen from the inset in FIG. 8 (b), when the dispersed phase flow rate was 36mL/h, the prepared W/O emulsion droplets were uniform in size and had an average diameter of 359.84. Mu.m.
Claims (9)
1. A rotary microfluidic device for controllably preparing monodisperse emulsion is characterized by comprising a rotary platform (1), an injection tube (2), a collection container (3) and an injection pump (4);
the rotary platform (1) comprises a motor and a horizontally arranged rotary table, and the motor drives the rotary table to rotate around the circle center of the rotary table at a constant speed; the collecting container (3) is horizontally fixed on the turntable, and the circle center of the collecting container (3) is superposed with that of the turntable; an inlet of the injection pipe (2) is communicated with the injection pump (4), the injection pipe (2) is arranged perpendicular to a horizontal plane, an outlet of the injection pipe (2) is located outside the circle center of the collecting container (3), an outlet of the injection pipe (2) is located below the liquid level of fluid contained in the collecting container, and the inner diameter of the outlet of the injection pipe (2) is 10 to 1000 micrometers.
2. A rotary microfluidic device for controllably preparing a monodisperse emulsion according to claim 1, wherein the device further comprises a fluid distribution plate (6), the fluid distribution plate is provided with a fluid inlet (6-1) and a plurality of fluid outlets, the distance between the fluid inlet and each fluid outlet is equal, the fluid inlet and each fluid outlet are communicated by a fluid channel (6-2), the fluid distribution plate is horizontally arranged, the fluid inlet is communicated with an injection pump, each fluid outlet is respectively communicated with the inlet of each injection tube (2), and the distance between the outlet of each injection tube and the center of the collection container is equal.
3. A rotary microfluidic device for the controlled preparation of monodisperse emulsions according to claim 2 wherein the fluid outlets of the fluid distribution plate (6) are evenly distributed around the fluid inlet (6-1).
4. A rotary microfluidic device for the controlled preparation of monodisperse emulsions according to any of claims 1 to 3 wherein the cross-section of the collection reservoir is circular.
5. A controllable preparation method of monodisperse emulsion based on rotary micro-fluidic device, which is characterized in that the method adopts the rotary micro-fluidic device of any claim 1 to 4 to prepare monodisperse emulsion, and comprises the following steps:
the continuous phase fluid is contained in a collecting container, and the motor drives a turntable to drive the collecting container and the continuous phase fluid therein to rotate at a constant speed around the center of a circle of the collecting container; then, the dispersed phase fluid is injected into the continuous phase fluid in the collection container through the injection pipe by the injection pump, so that monodisperse emulsion droplets can be prepared through liquid phase shearing, or the dispersed phase fluid is injected and distributed into each injection pipe through the fluid inlet of the fluid distribution plate by the injection pump and then flows into the continuous phase fluid in the collection container through the injection pipe, so that the monodisperse emulsion droplets can be prepared through liquid phase shearing;
the rotation speed of the motor and the flow rate of the dispersed phase fluid are controlled in the preparation process, so that the continuous phase fluid can shear and separate the dispersed phase fluid from the outlet of the injection tube through rotary shearing to form emulsion droplets.
6. A controlled preparation method of monodisperse emulsion according to claim 5 wherein the dispersed phase fluid and the continuous phase fluid are two-phase fluids that are immiscible or slightly soluble in each other.
7. The controllable preparation method of monodisperse emulsion based on rotary microfluidic device according to claim 6, characterized in that the aqueous phase solution is used as disperse phase fluid and the oil phase solution is used as continuous phase fluid; or taking the oil phase solution as a dispersed phase fluid and the water phase solution as a continuous phase fluid; or, taking liquid metal as dispersed phase fluid and taking water phase solution or oil phase solution as continuous phase fluid; or two water phase solutions which are not soluble or slightly soluble with each other are respectively used as a dispersed phase fluid and a continuous phase fluid; alternatively, two oil phase solutions which are insoluble or slightly soluble with each other are used as the dispersed phase fluid and the continuous phase fluid, respectively.
8. The controllable preparation method of monodisperse emulsion based on rotary microfluidic device according to claim 7, wherein the continuous phase fluid contains interface stabilizing agent, and the interface stabilizing agent comprises surfactant or nano-particles for interface stabilization.
9. The controllable preparation method of monodisperse emulsion based on rotary micro-fluidic device according to claim 7, characterized in that the aqueous phase solution or/and the oil phase solution contains at least one of functional polymer, monomer, thickener, salt, and functional nano-particles that can be dissolved in the aqueous phase solution or the oil phase solution.
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