CN114404672B - Fiber membrane combining polyphenol and antibacterial peptide as well as preparation method and application of fiber membrane - Google Patents
Fiber membrane combining polyphenol and antibacterial peptide as well as preparation method and application of fiber membrane Download PDFInfo
- Publication number
- CN114404672B CN114404672B CN202210058176.7A CN202210058176A CN114404672B CN 114404672 B CN114404672 B CN 114404672B CN 202210058176 A CN202210058176 A CN 202210058176A CN 114404672 B CN114404672 B CN 114404672B
- Authority
- CN
- China
- Prior art keywords
- fiber membrane
- polyphenol
- antibacterial peptide
- fibrous membrane
- polyphenol compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/02—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from cellulose, cellulose derivatives, or proteins
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
- D01F8/14—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one polyester as constituent
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
- D06M13/224—Esters of carboxylic acids; Esters of carbonic acid
- D06M13/238—Tannins, e.g. gallotannic acids
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
- D06M15/01—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with natural macromolecular compounds or derivatives thereof
- D06M15/15—Proteins or derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/216—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with other specific functional groups, e.g. aldehydes, ketones, phenols, quaternary phosphonium groups
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/45—Mixtures of two or more drugs, e.g. synergistic mixtures
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/10—Animal fibres
- D06M2101/14—Collagen fibres
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/16—Synthetic fibres, other than mineral fibres
- D06M2101/30—Synthetic polymers consisting of macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- D06M2101/32—Polyesters
Abstract
The invention discloses a fiber membrane combining polyphenol and antibacterial peptide as well as a preparation method and application thereof, belonging to the technical field of fiber membranes, wherein the fiber membrane is prepared by modifying the surface of a fiber membrane substrate by combining polyphenol compounds and antibacterial peptide; wherein the polyphenol compound grafts the antibacterial peptide to the surface of the fibrous membrane through non-covalent binding. According to the invention, the antibacterial peptide can be further grafted through the polyphenol compound, the rich phenolic hydroxyl groups of the polyphenol compound can form a strong non-covalent bond effect with the antibacterial peptide, and the consumption of the phenolic hydroxyl groups of the polyphenol compound after the antibacterial peptide is grafted can obviously reduce the cytotoxicity caused by the polyphenol compound; the combination of polyphenol compounds and antibacterial peptides can endow the fibrous membrane with better antibacterial performance, and can actively adjust local immune microenvironment around the implanted material, thereby effectively realizing H-shaped vascularized bone tissue regeneration.
Description
Technical Field
The invention relates to the technical field of fiber membranes, in particular to a fiber membrane combining polyphenol and antibacterial peptide, and a preparation method and application thereof.
Background
Bone tissue regeneration is a complex process involving interdisciplinary multidisciplines such as materials science and biomedicine. Bone tissue scaffold materials have been widely recognized as useful for the repair and regeneration of bone tissue. At present, researchers have designed and developed scaffold materials with different performances for bone tissue regeneration, but the realization of vascularized bone tissue regeneration still faces a lot of scientific problems and technical bottlenecks to be solved. The electrostatic spinning technology is widely applied to the field of tissue engineering because the prepared fibrous membrane has a typical extracellular matrix-like structure, and has unique application in the field of bone tissue engineering. The electrostatic spinning fiber membrane can be used as a barrier membrane for guiding regeneration of oral alveolar bones, and can also be used as a periosteum for promoting bone tissue healing and nerve regeneration in orthopedics. Due to the complexity of clinical problems, electrospun fibrous membranes have more stringent performance requirements for use in bone tissue regeneration. Therefore, the functional surface modification of the electrostatic spinning fiber membrane becomes an effective strategy for improving the comprehensive performance of the fiber membrane.
Currently, common methods of electrospun fiber membrane modification include coating, mixing, and covalent bonding. The conventional method of surface grafting by polydopamine has been widely used, but the problems are not negligible, such as that dark brown polydopamine generally changes the optical properties of the matrix material, and the abundant amino groups in polydopamine also interfere with the quantification of the ligand. In recent years, some new fiber membrane modification and grafting strategies have been developed, such as modification and grafting with functional binding peptides, however, their high cost and selectivity of the binding peptides to the substrate limit their wider application.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a fibrous membrane combining polyphenol and antibacterial peptide, a preparation method and application thereof, which can effectively solve the problem that H-shaped vascularized bone tissue regeneration cannot be realized in the existing fibrous membrane modification method.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a fiber membrane combining polyphenol and antibacterial peptide, which is prepared by modifying the surface of a fiber membrane substrate by combining polyphenol compounds and antibacterial peptide; wherein the polyphenol compound grafts the antibacterial peptide to the surface of the fibrous membrane through non-covalent binding.
The invention has the beneficial effects that: according to the invention, the antibacterial peptide can be further grafted through the polyphenol compound, the rich phenolic hydroxyl groups of the polyphenol compound can form a strong non-covalent bond effect with the antibacterial peptide, and the consumption of the phenolic hydroxyl groups of the polyphenol compound after the antibacterial peptide is grafted can obviously reduce the cytotoxicity caused by the polyphenol compound; the combination of polyphenol compounds and antibacterial peptides can endow the fibrous membrane with better antibacterial performance, and can actively adjust local immune microenvironment around the implant material, thereby effectively realizing H-type vascularization bone tissue regeneration.
Furthermore, the fiber membrane substrate is prepared by electrostatic spinning of high molecular polymer and bioactive components.
The beneficial effect of adopting the further scheme is that: the method for modifying the electrostatic spinning fiber membrane provided by the invention can simply and effectively graft the functional antibacterial peptide to the surface of the polymer-based fiber membrane material, and the polyphenol compound can form strong interaction with the surface of the polymer-based fiber membrane due to abundant phenolic hydroxyl groups of the polyphenol compound, has wide adaptability to the polymer-based fiber membrane, can be effectively combined with most of synthetic polymers and all natural polymer materials, and effectively solves the problems of poor adaptability to the polymer-based fiber membrane, high cost and the like in the conventional method for modifying the fiber membrane.
Further, the main component of the fiber membrane substrate prepared by electrostatic spinning is synthetic high molecular polymer or natural high molecular polymer, which widely comprises polycaprolactone, polylactic acid-glycolic acid, polyurethane, polytetrafluoroethylene, polyvinylidene fluoride, chitosan, collagen, gelatin and derivatives thereof.
Further, the structure of the fiber membrane substrate includes a uniaxial fiber membrane, a coaxial fiber membrane of a core-shell structure, or a multiaxial structure fiber membrane.
Further, bioactive components are added into the fiber membrane substrate, and the bioactive components comprise at least one of baicalin, baicalein, fish collagen, nano hydroxyapatite and calcium phosphate salt.
The beneficial effect of adopting the further scheme is that: the bioactive components can improve biocompatibility and anti-inflammatory effect of the fibrous membrane and promote bone function.
Further, the polyphenol compound is one or both of tannic acid and epigallocatechin gallate.
The beneficial effect of adopting the further scheme is that: the polyphenol compound has rich phenolic hydroxyl groups, and can effectively graft the antibacterial peptide to the surface of a fiber membrane substrate material through non-covalent binding.
Further, the antibacterial peptide with multiple functionalities is one or two of LL-37 and GL 13K.
The beneficial effect of adopting the further scheme is that: the antibacterial peptide can obviously weaken the cytotoxicity caused by polyphenol compounds, can endow the fibrous membrane with better antibacterial performance, and can actively adjust the local immune microenvironment around the implanted material, thereby effectively realizing the regeneration of H-type vascularized bone tissues.
Further, the polyphenol compound and the antibacterial peptide are in non-covalent combination.
The invention also provides a preparation method of the fiber membrane combining polyphenol and antibacterial peptide, which comprises the following steps:
s1, dispersing bioactive components in an organic solvent, adding a high molecular compound, shaking up to obtain a spinning solution, preparing a fiber membrane substrate through electrostatic spinning, and soaking the obtained fiber membrane substrate in an alcohol solvent;
s2, placing the fiber membrane substrate obtained in the S1 in a polyphenol compound solution, standing and storing, taking out, and washing to obtain a fiber membrane grafted with a polyphenol compound;
and S3, placing the fiber membrane grafted with the polyphenol compound obtained in the step S2 into an aqueous solution of antibacterial peptide, standing at the temperature of 2-10 ℃, taking out after storage, and washing to obtain the fiber membrane combined with polyphenol and antibacterial peptide.
Further, the organic solvent in step S1 is trifluoroethanol, hexafluoroisopropanol, acetone, trifluoroacetic acid, dichloromethane or N, N-dimethylformamide.
Further, the alcohol solvent in step S1 is preferably 75 ± 5% ethanol, and is soaked for 20 to 40 minutes.
Further, the electrostatic spinning process parameters in the step S1 are set as follows: the voltage is 4-20kV, the bolus injection speed is 0.2-1mL/h, and the receiving distance is 10-20cm; wherein the preferable process parameters are 6-10kV of voltage, 0.3-0.6mL/h of bolus injection speed and 14-17cm of receiving distance.
Further, the concentration of the polyphenol compound solution in the step S2 is 1-50mg/ml, and the standing and storing time is 1-12 hours; wherein the preferable conditions are that the concentration of polyphenol compound solution is 5-25mg/ml, and the standing and storing time is 4-8 hours.
Further, the concentration of the antibacterial peptide solution in the step S3 is 0.5-10mg/ml, and the standing and storing time is 1-24 hours; wherein the preferable conditions are that the concentration of the antibacterial peptide solution is 1-3mg/ml, and the standing and storing time is 4-8 hours.
The preparation method has the beneficial effects that: the polymer-based fiber membrane is used as a substrate material, plays an important role in the physical and chemical properties, the degradation performance, the biocompatibility and the like of the whole material system, and can select a corresponding polymer substrate material according to a specific application scene; the content of polyphenol compounds plays an extremely important role in the process of modifying a polymer substrate, the electrostatic spinning fiber membrane is soaked in a polyphenol solution for too long time, a large amount of polyphenol compounds are enriched on the surface of the fiber membrane, cytotoxicity is easily caused, and the electrostatic spinning fiber membrane cannot effectively interact with the polymer substrate material and further graft antibacterial peptide when the electrostatic spinning fiber membrane is soaked in the polyphenol solution for too short time; the content of the antibacterial peptide mainly influences the biological properties of the fibrous membrane such as antibacterial property, cell compatibility, immunoregulation property, and vascularization and osteogenesis promoting effect, and the functional characteristics cannot be realized if the content is too high or too low. Therefore, the invention solves the problem that the regeneration of H-type vascularized bone tissues cannot be realized in the existing modification method of the fibrous membrane through the mutual synergistic interaction of the polymer substrate fibrous membrane, the polyphenol and the antibacterial peptide.
The invention also provides application of the fiber membrane combining polyphenol and antibacterial peptide in preparation and/or application as an H-type vascularized bone tissue regeneration material.
In summary, the invention has the following advantages:
1. the method for modifying the electrostatic spinning fiber membrane can simply and effectively graft the functional antibacterial peptide to the surface of the polymer-based fiber membrane material, and the polyphenol compound can form strong interaction with the surface of the polymer-based fiber membrane due to abundant phenolic hydroxyl groups of the polyphenol compound, has wide adaptability to the polymer-based fiber membrane, and can be effectively combined with most of synthetic polymers and all natural polymer materials; the antibacterial peptide can be further grafted by the polyphenol compound, and the rich phenolic hydroxyl of the polyphenol compound can form stronger non-covalent bond effect with the antibacterial peptide.
In addition, after the fiber membrane is grafted with the antibacterial peptide, the phenolic hydroxyl of the polyphenol compound is consumed, so that the cytotoxicity caused by the polyphenol compound can be obviously weakened, cell proliferation and differentiation are facilitated, and further, the regeneration of bone tissues is promoted.
In addition, the combination of polyphenol compounds and antibacterial peptides can endow the fiber membrane with better antibacterial performance.
In addition, after the antibacterial peptide is grafted on the surface of the fibrous membrane, the local immune microenvironment around the implanted material can be actively regulated, and then H-shaped vascularized bone tissue regeneration can be effectively realized.
In addition, the surface of the fibrous membrane can be further loaded with anti-inflammatory factors or growth factors, such as IL-4, BMP-2 and the like.
The fibrous membrane combining polyphenol and antibacterial peptide provided by the invention has a typical extracellular matrix-like structure, and the preparation method has the advantages of stable and repeatable technical process, simple operation and easiness in large-scale production.
Drawings
FIG. 1 shows the results of soaking a fiber membrane of the present invention with non-grafted tannic acid (FIG. 1, left) and grafted tannic acid (FIG. 1, right) on the surface in silver nitrate solution;
FIG. 2 is a scanning electron microscope image of a fiber membrane modified with tannic acid and LL-37 polypeptide of the invention;
FIG. 3 is a scanning electron micrograph of E.coli of the present invention after cultured on a fiber membrane modified with tannic acid and LL-37 polypeptide;
FIG. 4 is a scanning electron micrograph of Staphylococcus aureus in the present invention after cultured on a fiber membrane modified with tannic acid and LL-37 polypeptide;
FIG. 5 is a graph showing the effect of tannic acid and LL-37 polypeptide modified fibrous membrane in inducing the regeneration of rat skull in the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the detailed description and specific examples, while indicating embodiments of the invention, are given by way of illustration only, not by way of limitation, i.e., the embodiments described are intended as a selection of the best mode contemplated for carrying out the invention, not as a full mode.
Thus, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for preparing fiber membrane combining polyphenol and antibacterial peptide comprises preparing polylactic acid-glycolic acid from Shandong Jinan Dai handle biological science and technology Co., ltd, fish collagen from bio-corporation Co., ltd, tannic acid and hexafluoroisopropanol from Aladdin, LL-37 peptide from Nanjing Jie peptide biological science and technology Co., ltd, and comprises the following steps:
step 1: weighing 0.04g of fish collagen, dispersing in 1mL of hexafluoroisopropanol, weighing 0.2g of polylactic acid-glycolic acid, adding into the obtained dispersion, shaking uniformly for 60min by using a constant-temperature oscillator to obtain a spinning solution, preparing a fish collagen/polylactic acid-glycolic acid fiber membrane by electrostatic spinning, wherein the spinning voltage is 8kV, the injection speed is 0.5mL/h, the receiving distance is 15cm, and placing the electrostatic spinning fiber membrane in a vacuum drying oven for drying for one week;
and 2, step: soaking the fiber membrane obtained in the step 1 in 75% ethanol for 30min;
and 3, step 3: placing the fiber membrane obtained in the step 2 in a tannic acid solution with the concentration of 40mg/ml, standing and storing for 2 hours, taking out, and slightly washing with deionized water twice to obtain a fiber membrane grafted with tannic acid;
and 4, step 4: and (4) placing the fiber membrane grafted with the tannin obtained in the step (3) into a solution with the concentration of LL-37 peptide of 2mg/ml, standing and storing for 6 hours at the temperature of 4 ℃, taking out the fiber membrane, and slightly washing the fiber membrane with deionized water twice to obtain the fiber membrane modified by the tannin and the LL-37 peptide.
Example 2
A method for preparing a fibrous membrane combining polyphenol and antibacterial peptide comprises the following steps of preparing raw materials of polylactic acid-glycolic acid of Shandong Jinan Dai handle biological science and technology Limited company, polycaprolactone of Shenzhen Guanghua Weber limited company, fish collagen of Sheng biological science and technology Limited company, tannic acid, baicalin, hexafluoroisopropanol and trifluoroethanol of Aladdin company, and LL-37 peptide of Nanjing Jie peptide biological science and technology Limited company:
step 1: weighing 0.015g of baicalin, dispersing in 1mL of trifluoroethanol, weighing 0.12g of polycaprolactone, adding the polycaprolactone into the obtained dispersion liquid, shaking and shaking uniformly for 45min by using a constant-temperature oscillator to obtain a spinning solution A, weighing 0.08g of fish collagen, dispersing in 2mL of hexafluoroisopropanol, weighing 0.4g of polylactic acid-glycolic acid, adding the polylactic acid-glycolic acid into the obtained dispersion liquid, and shaking uniformly for 60min by using a constant-temperature oscillator to obtain a spinning solution B;
step 2: respectively filling the spinning solutions A and B obtained in the step 1 into 5mL injectors, respectively placing the injectors into two injection devices of an electrostatic spinning machine, respectively connecting the spinning solutions A and B with the inner layer and the outer layer of a coaxial needle, preparing a fish collagen/polylactic acid-glycolic acid/polycaprolactone-baicalin composite fiber membrane through electrostatic spinning, adopting a flat receiver, wherein the spinning voltage is 7kV, the injection speeds of the spinning solutions A and B are respectively 0.2mL/h and 0.4mL/h, and the receiving distance is 15cm;
and 3, step 3: placing the fiber membrane obtained in the step 2 in a vacuum drying oven for drying for one week, and then placing the fiber membrane in 75% ethanol for soaking for 30min;
and 4, step 4: placing the fiber membrane obtained in the step 3 in a tannic acid solution with the concentration of 10mg/ml, standing and storing for 2 hours, taking out, and slightly washing with deionized water twice to obtain a fiber membrane grafted with tannic acid;
and 5: and (3) placing the fiber membrane grafted with the tannic acid obtained in the step (4) into a solution of LL-37 peptide with the concentration of 2mg/ml, standing at 4 ℃ for 6 hours, taking out, and slightly washing with deionized water twice to obtain the fiber membrane modified by the tannic acid and the LL-37 peptide.
Example 3
A method for preparing a fibrous membrane combining polyphenol and antibacterial peptide comprises the following steps of preparing raw materials of polylactic acid-glycolic acid of Shandong Jinan Dai handle biological science and technology Limited company, polycaprolactone of Shenzhen Guanghua Weber limited company, fish collagen of Sheng biological science and technology Limited company, tannic acid, baicalin, hexafluoroisopropanol and trifluoroethanol of Aladdin company, and LL-37 peptide of Nanjing Jie peptide biological science and technology Limited company:
step 1: weighing 0.015g of baicalin, dispersing in 1mL of trifluoroethanol, weighing 0.12g of polycaprolactone, adding the polycaprolactone into the obtained dispersion liquid, shaking and shaking uniformly for 45min by using a constant-temperature oscillator to obtain a spinning solution A, weighing 0.08g of fish collagen, dispersing in 2mL of hexafluoroisopropanol, weighing 0.4g of polylactic acid-glycolic acid, adding the polylactic acid-glycolic acid into the obtained dispersion liquid, and shaking uniformly for 60min by using a constant-temperature oscillator to obtain a spinning solution B;
and 2, step: respectively filling the spinning solutions A and B obtained in the step 1 into 5mL injectors, respectively placing the injectors into two injection devices of an electrostatic spinning machine, respectively connecting the spinning solutions A and B with the inner layer and the outer layer of a coaxial needle, preparing a fish collagen/polylactic acid-glycolic acid/polycaprolactone-baicalin composite fiber membrane through electrostatic spinning, adopting a flat receiver, wherein the spinning voltage is 7kV, the injection speeds of the spinning solutions A and B are respectively 0.2mL/h and 0.4mL/h, and the receiving distance is 15cm;
and step 3: drying the fiber membrane obtained in the step 2 in a vacuum drying oven for one week, and soaking in 75% ethanol for 30min;
and 4, step 4: placing the fiber membrane obtained in the step 3 in a tannic acid solution with the concentration of 5mg/ml, standing and storing for 4 hours, taking out, and slightly washing with deionized water twice to obtain the tannic acid grafted fiber membrane;
and 5: and (3) placing the fiber membrane grafted with the tannin obtained in the step (4) into a solution with the concentration of LL-37 peptide of 1mg/ml, standing and storing for 12 hours at the temperature of 4 ℃, taking out the fiber membrane, and slightly washing the fiber membrane with deionized water twice to obtain the fiber membrane modified by the tannin and the LL-37 peptide.
Example 4
A method for preparing a fibrous membrane combining polyphenol and antibacterial peptide comprises the following steps of preparing raw materials of polylactic acid-glycolic acid of Shandong Jinan Dai handle biological science and technology Limited company, polycaprolactone of Shenzhen Guanghua Weber limited company, fish collagen of Sheng biological science and technology Limited company, tannic acid, baicalin, hexafluoroisopropanol and trifluoroethanol of Aladdin company, and LL-37 peptide of Nanjing Jie peptide biological science and technology Limited company:
step 1: weighing 0.015g of baicalin to disperse in 1mL of trifluoroethanol, weighing 0.12g of polycaprolactone to add into the obtained dispersion liquid, shaking and shaking uniformly for 45min by using a constant temperature oscillator to obtain a spinning solution A, weighing 0.08g of fish collagen to disperse in 2mL of hexafluoroisopropanol, weighing 0.4g of polylactic acid-glycolic acid to add into the obtained dispersion liquid, and shaking uniformly for 60min by using a constant temperature oscillator to obtain a spinning solution B;
step 2: respectively filling the spinning solutions A and B obtained in the step 1 into 5mL injectors, respectively placing the injectors into two injection devices of an electrostatic spinning machine, respectively connecting the spinning solutions A and B with the inner layer and the outer layer of a coaxial needle, preparing a fish collagen/polylactic acid-glycolic acid/polycaprolactone-baicalin composite fiber membrane through electrostatic spinning, adopting a flat receiver, wherein the spinning voltage is 7kV, the injection speeds of the spinning solutions A and B are respectively 0.2mL/h and 0.4mL/h, and the receiving distance is 15cm;
and step 3: placing the fiber membrane obtained in the step 2 in a vacuum drying oven for drying for one week, and then placing the fiber membrane in 75% ethanol for soaking for 30min;
and 4, step 4: placing the fiber membrane obtained in the step 3 in a tannic acid solution with the concentration of 1mg/ml, standing and storing for 2 hours, taking out, and slightly washing with deionized water twice to obtain a tannic acid grafted fiber membrane;
and 5: and (3) placing the fiber membrane grafted with the tannic acid obtained in the step (4) into a solution of LL-37 peptide with the concentration of 0.5mg/ml, standing at 4 ℃ for 6 hours, taking out, and slightly washing with deionized water twice to obtain the fiber membrane modified by the tannic acid and the LL-37 peptide.
Experimental example 1 detection of grafted tannic acid
In this example, the fiber membrane obtained in example 3 was examined as follows: placing fish collagen/polylactic acid-glycolic acid/polycaprolactone-baicalin fiber membrane in tannic acid solution with concentration of 5mg/ml, standing for 4 hr, taking out, slightly washing with deionized water twice to obtain fiber membrane grafted with tannic acid, and further placing the fiber membrane grafted with tannic acid and ungrafted with tannic acid in 100mM AgNO 3 The solution was allowed to stand for 12 hours. As shown in fig. 1 (in which fig. 1 is a photograph of a fiber membrane with the surface not grafted with tannic acid (fig. 1 left) and the surface grafted with tannic acid (fig. 1 right) after soaking in a silver nitrate solution), a tan precipitate was generated on the surface of the fiber membrane grafted with tannic acid, indicating that tannic acid was successfully grafted on the surface of the fiber membrane.
Experimental example 2 morphology and Structure Observation
In this example, the fibrous membrane obtained in example 3 was observed by using a scanning electron microscope, and the morphology of the fish collagen/polylactic acid-glycolic acid/polycaprolactone-baicalin fibrous membrane modified by tannic acid and LL-37 peptide was determined, and as shown in FIG. 2, the fibrous membrane had a typical extracellular matrix-like structure, and particles of LL-37 peptide were grafted on the surface of the fiber.
Experimental example 3 antibacterial Properties
In this example, a scanning electron microscope is used to perform an antibacterial performance test on the fiber film obtained in example 3, and the specific steps are as follows:
step 1: placing a disc-shaped fibrous membrane with the diameter of 1cm in a 24-hole cell culture plate;
step 2: inoculating staphylococcus aureus and escherichia coli to a fiber membrane according to the density of 106CFU/mL, and culturing for 2 hours;
and step 3: absorbing and removing the culture solution, slightly washing twice with PBS, and performing gradient dehydration with a mixed solution of ethanol and tert-butyl alcohol;
and 4, step 4: and observing the shape of bacteria on the fiber membrane through a scanning electron microscope after gold spraying.
As shown in FIGS. 3 and 4, the bacterial structures of Staphylococcus aureus and Escherichia coli are damaged to a certain extent on the surfaces of the fiber membranes modified by tannic acid and LL-37 peptide, and the fiber membranes are modified by the surfaces of the Staphylococcus aureus and the Escherichia coli in combination with the tannic acid and the LL-37 peptide, so that the fiber membranes have good antibacterial performance.
Experimental example 5 measurement of bone tissue regeneration Performance
A fibrous membrane modified by tannic acid and LL-37 polypeptide (example 3) is implanted into a rat skull limit defect model, the skull repairing effect is observed after 12 weeks, and the Micro-CT reconstruction result is shown in figure 5, wherein a large amount of new bone formation exists in a defect area and almost completely covers the defect area, so that the prepared fibrous membrane modified by polyphenol and antibacterial peptide has a good bone tissue regeneration guiding effect and has a wide application prospect in the field of bone tissue engineering.
In the embodiment, on the basis of preparing the fiber membrane in the embodiment 3, the bone tissue regeneration performance of the obtained fiber membrane is inspected by singly changing the soaking time of the fiber membrane in the polyphenol solution and the content of the antibacterial peptide by a variable control method, and the finding shows that the too long soaking time (more than 12 hours) in the polyphenol solution is very easy to cause cytotoxicity, and the too short soaking time (less than 1 hour) in the polyphenol solution cannot effectively interact with a polymer substrate material and further graft the antibacterial peptide, so that the bone tissue regeneration performance of the obtained fiber membrane is greatly reduced; too high (more than 10 mg/ml) or too low (less than 0.5 mg/ml) content of the antibacterial peptide can affect the biological properties of the fibrous membrane, such as antibacterial property, cell compatibility, immunoregulation, vascularization promotion and bone formation effect, and the like, and reduce the bone tissue regeneration performance of the obtained fibrous membrane.
The foregoing is merely exemplary and illustrative of the present invention and it is within the purview of one skilled in the art to modify or supplement the embodiments described or to substitute similar ones without the exercise of inventive faculty, and still fall within the scope of the claims.
Claims (7)
1. The fibrous membrane combining polyphenol and antibacterial peptide is characterized by being prepared by modifying the surface of a fibrous membrane substrate by combining polyphenol compound and antibacterial peptide; wherein the polyphenol compound is combined by a non-covalent mode to graft the antibacterial peptide to the surface of the fibrous membrane;
the main component of the fibrous membrane substrate is a high molecular polymer, and the high molecular polymer comprises polycaprolactone, polylactic acid-glycolic acid, polyurethane, polytetrafluoroethylene, polyvinylidene fluoride, chitosan, collagen, gelatin and derivatives thereof;
the polyphenol compound is one or two of tannic acid and epigallocatechin gallate;
the antibacterial peptide is one or two of LL-37 and GL 13K.
2. A fibrous membrane combining polyphenols and antimicrobial peptides according to claim 1, wherein the fibrous membrane substrate is prepared by electrostatic spinning of a high molecular weight polymer and a bioactive ingredient.
3. A fibrous membrane that combines polyphenols and antimicrobial peptides according to claim 2, wherein a bioactive ingredient is further added to the fibrous membrane substrate, the bioactive ingredient comprising at least one of baicalin, baicalein, fish collagen, nano-hydroxyapatite, and calcium phosphate salts.
4. A method of preparing a fibrous membrane that combines polyphenols and antimicrobial peptides according to any of claims 1 to 3, characterised in that it comprises the following steps:
s1, preparing a fiber membrane substrate through electrostatic spinning, and soaking the obtained fiber membrane substrate in an alcohol solvent;
s2, placing the fiber membrane substrate obtained in the S1 in a polyphenol compound solution, standing and storing, taking out, and washing to obtain a fiber membrane grafted with a polyphenol compound;
and S3, placing the fiber membrane grafted with the polyphenol compound obtained in the step S2 into an aqueous solution of antibacterial peptide, standing at the temperature of 2-10 ℃, taking out after storage, and washing to obtain the fiber membrane combined with polyphenol and antibacterial peptide.
5. The method for preparing a fibrous membrane combining polyphenol and antimicrobial peptide according to claim 4, wherein the organic solvent in the step S1 is trifluoroethanol or hexafluoroisopropanol or acetone or trifluoroacetic acid or dichloromethane or N, N-dimethylformamide; the electrostatic spinning process parameters in the step S1 are set as follows: the voltage is 4-20kV, the bolus injection speed is 0.2-1mL/h, and the receiving distance is 10-20cm.
6. The method for preparing a fibrous membrane in combination with polyphenol and antimicrobial peptide according to claim 4, wherein the concentration of the solution of tannic acid in step S2 is 1 to 50mg/ml, and the standing and storing time is 1 to 12 hours; the concentration of the antibacterial peptide solution in the step S3 is 0.5-10mg/ml, and the standing and storing time is 6-24 hours.
7. Use of a fibrous membrane combining polyphenols and antimicrobial peptides according to any one of claims 1 to 3 in the preparation of a H-type vascularized bone tissue regeneration material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210058176.7A CN114404672B (en) | 2022-01-19 | 2022-01-19 | Fiber membrane combining polyphenol and antibacterial peptide as well as preparation method and application of fiber membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210058176.7A CN114404672B (en) | 2022-01-19 | 2022-01-19 | Fiber membrane combining polyphenol and antibacterial peptide as well as preparation method and application of fiber membrane |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114404672A CN114404672A (en) | 2022-04-29 |
| CN114404672B true CN114404672B (en) | 2023-03-03 |
Family
ID=81274270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210058176.7A Active CN114404672B (en) | 2022-01-19 | 2022-01-19 | Fiber membrane combining polyphenol and antibacterial peptide as well as preparation method and application of fiber membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114404672B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113967414A (en) * | 2020-07-24 | 2022-01-25 | 滁州学院 | Composite nanofiltration membrane and preparation method and application thereof |
| CN115970068A (en) * | 2022-09-16 | 2023-04-18 | 四川大学 | Heart plugging device with tissue induction and regeneration function and preparation method thereof |
| CN115606596B (en) * | 2022-11-02 | 2024-02-23 | 中国石油大学(华东) | Reticular antibacterial material, preparation method and application thereof |
| CN117338628B (en) * | 2023-12-05 | 2024-04-09 | 杭州湃肽生化科技有限公司 | Skin tightening and anti-wrinkle composition and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106983912A (en) * | 2017-04-17 | 2017-07-28 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of anti-bacterial hydrogel recovery support of 3D printing and preparation method thereof |
| CN110025826A (en) * | 2019-05-08 | 2019-07-19 | 苏州大学附属第二医院 | Inducting osseous tissue regeneration film, preparation method and application |
| CN113462000A (en) * | 2021-06-21 | 2021-10-01 | 武汉大学 | Surface antibacterial treatment method of absorbable surgical material |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2432388T3 (en) * | 2008-12-05 | 2013-12-03 | Semprus Biosciences Corporation | Anti-fouling, antimicrobial and antithrombogenic graft compositions from the surface |
| EP2533772B1 (en) * | 2010-02-10 | 2021-09-08 | Imbed Biosciences, Inc. | Methods and compositions for wound healing |
| WO2016022796A1 (en) * | 2014-08-08 | 2016-02-11 | Surmodics, Inc. | Article coatings including oligomerized polyphenol layer and biological methods of use |
| CA3009754C (en) * | 2015-01-29 | 2020-11-10 | Mark R. Wardell | Wound healing composition comprising buckwheat honey and methylglyoxal, and methods of use |
| US20160303281A1 (en) * | 2015-04-17 | 2016-10-20 | Rochal Industries, Llc | Composition and kits for pseudoplastic microgel matrices |
| CN107693843B (en) * | 2017-04-01 | 2020-09-04 | 华东理工大学 | Surface modification method of biomedical active titanium and alloy implant material thereof |
-
2022
- 2022-01-19 CN CN202210058176.7A patent/CN114404672B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106983912A (en) * | 2017-04-17 | 2017-07-28 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of anti-bacterial hydrogel recovery support of 3D printing and preparation method thereof |
| CN110025826A (en) * | 2019-05-08 | 2019-07-19 | 苏州大学附属第二医院 | Inducting osseous tissue regeneration film, preparation method and application |
| CN113462000A (en) * | 2021-06-21 | 2021-10-01 | 武汉大学 | Surface antibacterial treatment method of absorbable surgical material |
Non-Patent Citations (3)
| Title |
|---|
| A baicalin-loaded coaxial nanofiber scaffold regulated inflammation and osteoclast differentiation for vascularized bone regeneration;Shue Jin;《Bioactive Materials》;20210630;559-572 * |
| Natural polyphenols as versatile platforms for material engineering and surface functionalization;Xu liqun;《Progress in polymer science》;20181228;165-196 * |
| 钛纳米管表面固定GL13K抗菌肽的实验研究;王泽华;《安徽医科大学学报》;20190111;251-255 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114404672A (en) | 2022-04-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114404672B (en) | Fiber membrane combining polyphenol and antibacterial peptide as well as preparation method and application of fiber membrane | |
| Dadras Chomachayi et al. | Electrospun nanofibers comprising of silk fibroin/gelatin for drug delivery applications: thyme essential oil and doxycycline monohydrate release study | |
| Zhou et al. | Multifunctional and biomimetic fish collagen/bioactive glass nanofibers: Fabrication, antibacterial activity and inducing skin regeneration in vitro and in vivo | |
| Wang et al. | Stem cell-based tissue engineering with silk biomaterials | |
| CN103191085B (en) | A kind of two-layer compound regeneration membrane and preparation method thereof | |
| Cheng et al. | Promoting osteogenic differentiation in pre-osteoblasts and reducing tibial fracture healing time using functional nanofibers | |
| CN105688274B (en) | A kind of preparation process of polycaprolactone/gelatin electrospinning compound rest | |
| Cui et al. | Collagen-tussah silk fibroin hybrid scaffolds loaded with bone mesenchymal stem cells promote skin wound repair in rats | |
| Mandal et al. | Calcium alginate beads embedded in silk fibroin as 3D dual drug releasing scaffolds | |
| Moisenovich et al. | In vitro and in vivo biocompatibility studies of a recombinant analogue of spidroin 1 scaffolds | |
| Salehi et al. | Polyurethane/gelatin nanofibrils neural guidance conduit containing platelet-rich plasma and melatonin for transplantation of schwann cells | |
| CN103736153A (en) | Single-layer and double-layer polycaprolactone-based guided tissue regeneration membranes and preparation method thereof | |
| CN101601869A (en) | A kind of preparation method of collagen/bioglass/hyaluronic acid tissue repairing materials | |
| Kim et al. | Fabrication of duck’s feet collagen–silk hybrid biomaterial for tissue engineering | |
| CN110025826A (en) | Inducting osseous tissue regeneration film, preparation method and application | |
| Zheng et al. | Biofunctionalization of electrospun fiber membranes by LbL-collagen/chondroitin sulfate nanocoating followed by mineralization for bone regeneration | |
| Zha et al. | Electrospun natural polymer and its composite nanofibrous scaffolds for nerve tissue engineering | |
| CN101653624A (en) | Preparation method of composite nanometer fiber small-diameter intravascular tissue engineering stent material | |
| Zhang et al. | Helicobacter pylori ribosomal protein-A2 peptide/silk fibroin nanofibrous composites as potential wound dressing | |
| EP2681280B1 (en) | Biomaterial, method for making the biomaterial and uses of the same | |
| Dai et al. | Attapulgite-doped electrospun PCL scaffolds for enhanced bone regeneration in rat cranium defects | |
| Wu et al. | Biomimetic three-layered membranes comprising (poly)-ε-caprolactone, collagen and mineralized collagen for guided bone regeneration | |
| Liu et al. | 3D printed platelet-rich plasma-loaded scaffold with sustained cytokine release for bone defect repair | |
| Sun et al. | Enhanced cell affinity of the silk fibroin-modified PHBHHx material | |
| Movahedi et al. | Casein release and characterization of electrospun nanofibres for cartilage tissue engineering |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |