CN114403447A - A method for preparing plant extract treated with enzyme - Google Patents
A method for preparing plant extract treated with enzyme Download PDFInfo
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- CN114403447A CN114403447A CN202210126394.XA CN202210126394A CN114403447A CN 114403447 A CN114403447 A CN 114403447A CN 202210126394 A CN202210126394 A CN 202210126394A CN 114403447 A CN114403447 A CN 114403447A
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 121
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 121
- 239000000419 plant extract Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 16
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 105
- 238000000605 extraction Methods 0.000 claims abstract description 74
- 239000000284 extract Substances 0.000 claims abstract description 72
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 52
- 238000002360 preparation method Methods 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 29
- 238000005374 membrane filtration Methods 0.000 claims abstract description 27
- 238000000926 separation method Methods 0.000 claims abstract description 27
- 230000001954 sterilising effect Effects 0.000 claims abstract description 21
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 15
- 238000010438 heat treatment Methods 0.000 claims abstract description 14
- 230000000415 inactivating effect Effects 0.000 claims abstract description 14
- 239000008367 deionised water Substances 0.000 claims abstract description 13
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 13
- 238000003756 stirring Methods 0.000 claims abstract description 13
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 229940088598 enzyme Drugs 0.000 claims description 113
- 241000196324 Embryophyta Species 0.000 claims description 99
- 108010059892 Cellulase Proteins 0.000 claims description 14
- 108091005804 Peptidases Proteins 0.000 claims description 14
- 239000004365 Protease Substances 0.000 claims description 14
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 14
- 229940106157 cellulase Drugs 0.000 claims description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 claims description 13
- 108010059820 Polygalacturonase Proteins 0.000 claims description 8
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 8
- 239000000706 filtrate Substances 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000002699 waste material Substances 0.000 claims description 6
- 102000030523 Catechol oxidase Human genes 0.000 claims description 5
- 108010031396 Catechol oxidase Proteins 0.000 claims description 5
- 241000207199 Citrus Species 0.000 claims description 5
- 241000723377 Coffea Species 0.000 claims description 5
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 5
- 108010031186 Glycoside Hydrolases Proteins 0.000 claims description 5
- 102000005744 Glycoside Hydrolases Human genes 0.000 claims description 5
- 244000269722 Thea sinensis Species 0.000 claims description 5
- 235000020971 citrus fruits Nutrition 0.000 claims description 5
- 108010038851 tannase Proteins 0.000 claims description 5
- 230000002779 inactivation Effects 0.000 claims 2
- 230000009849 deactivation Effects 0.000 description 10
- 150000001875 compounds Chemical class 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 210000002421 cell wall Anatomy 0.000 description 3
- 238000004821 distillation Methods 0.000 description 2
- 238000002481 ethanol extraction Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/163—Liquid or semi-liquid tea extract preparations, e.g. gels, liquid extracts in solid capsules
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/166—Addition of, or treatment with, enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/20—Removing unwanted substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/20—Removing unwanted substances
- A23F3/205—Using flocculating or adsorbing agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/22—Drying or concentrating tea extract
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F5/00—Coffee; Coffee substitutes; Preparations thereof
- A23F5/16—Removing unwanted substances
- A23F5/18—Removing unwanted substances from coffee extract
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F5/00—Coffee; Coffee substitutes; Preparations thereof
- A23F5/16—Removing unwanted substances
- A23F5/18—Removing unwanted substances from coffee extract
- A23F5/185—Removing unwanted substances from coffee extract using flocculating, precipitating, adsorbing or complex-forming agents, or ion-exchangers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F5/00—Coffee; Coffee substitutes; Preparations thereof
- A23F5/24—Extraction of coffee; Coffee extracts; Making instant coffee
- A23F5/243—Liquid, semi-liquid or non-dried semi-solid coffee extract preparations; Coffee gels; Liquid coffee in solid capsules
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F5/00—Coffee; Coffee substitutes; Preparations thereof
- A23F5/24—Extraction of coffee; Coffee extracts; Making instant coffee
- A23F5/246—Addition of, or treatment with, enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F5/00—Coffee; Coffee substitutes; Preparations thereof
- A23F5/24—Extraction of coffee; Coffee extracts; Making instant coffee
- A23F5/28—Drying or concentrating coffee extract
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/21—Removal of unwanted matter, e.g. deodorisation or detoxification by heating without chemical treatment, e.g. steam treatment, cooking
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
- A23L5/276—Treatment with inorganic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/40—Colouring or decolouring of foods
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
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- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Dispersion Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Tea And Coffee (AREA)
Abstract
The invention discloses a method for preparing a plant extract treated by enzyme, which comprises the following steps: crushing plants, inactivating original enzymes, adding the plants into an extraction tank, adding ethyl acetate to perform initial extraction of plant components, and performing centrifugal separation and membrane filtration to obtain initial extract and plant residues; heating the plant residues to remove ethyl acetate extractant, adding the plant residues into a new extraction tank, adding deionized water, stirring uniformly, adding a complex enzyme preparation, performing enzymolysis, adding ethanol, performing continuous low-temperature extraction, and performing centrifugal separation and membrane filtration to obtain an enzyme extract and secondary plant residues; inactivating enzyme of the secondary enzyme extract, adding the primary extract into the secondary enzyme extract for mixing, heating in water bath, and distilling to remove ethyl acetate and ethanol to obtain mixed extract; adsorbing and decolorizing the extract by active carbon, removing the active carbon by centrifugal separation and membrane filtration, sterilizing at high temperature, and concentrating the extract to obtain the plant extract.
Description
Technical Field
The invention relates to the technical field of plant extract construction, in particular to a method for preparing a plant extract treated by enzyme.
Background
With the development of quality of life, more and more people pursue health preservation, health food is rapidly developed, wherein plant extracts can be extracted and concentrated by using nutrient components of plants to remove impurities, so that the food is convenient to eat and the taste is improved.
Disclosure of Invention
The present invention is directed to a method for producing an enzyme-treated plant extract, which solves the problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme: a method for producing an enzyme-treated plant extract, comprising the steps of:
s1, crushing the plants, inactivating original enzymes, adding the plants into an extraction tank, adding ethyl acetate to perform initial extraction of plant components, and performing centrifugal separation and membrane filtration to obtain initial extract and plant residues;
s2, heating the plant residues to remove ethyl acetate extractant, adding the plant residues into a new extraction tank, adding deionized water, stirring uniformly, adding a complex enzyme preparation, carrying out enzymolysis, adding ethanol, carrying out continuous low-temperature extraction, and carrying out centrifugal separation and membrane filtration to obtain enzyme extract and secondary plant residues;
s3, inactivating enzyme of the secondary enzyme extract, adding the primary extract into the secondary enzyme extract for mixing, and removing ethyl acetate and ethanol by heating and distilling in a water bath to obtain a mixed extract;
s4, adsorbing and decoloring the mixed extract by using active carbon, removing the active carbon by centrifugal separation and membrane filtration, sterilizing at high temperature, and concentrating the extract to obtain the plant extract.
In a preferred embodiment, in step S1, the weight ratio of ethyl acetate to plant weight is 3-5:1, the initial extraction temperature is 20-50 ℃, the stirring is carried out while the extraction is carried out, and the extraction time is 1-2 h.
In a preferred embodiment, in step S2, the addition amount of deionized water is 2-3 times of the plant residue, the enzymolysis temperature is 25-40 ℃, the enzymolysis time is 8-12h, the addition amount of ethanol is 30-40% of the mass of the mixed solution, the extraction temperature of the continuous low-temperature extraction is 20-30 ℃, the pressure is 0.30-1.2MPa, the extraction time is 20-50min, the continuous extraction is performed for 3-5 times, the secondary plant residue after the centrifugal separation and the membrane filtration is squeezed, the squeezed filtrate is mixed with the enzyme extract, and the residue is waste residue.
In a preferred embodiment, in step S3, the temperature of the water bath is 80-85 ℃, and the ethyl acetate and ethanol distilled by the water bath are condensed and recovered.
In a preferred embodiment, in step S4, the weight-to-volume (g/ml) ratio of the activated carbon to the mixed extract is 35-8:100, and the sterilization is performed by high-temperature instantaneous sterilization at 125-145 ℃ for 5-10S.
In a preferred embodiment, the enzyme extinguishing method adopts microwave enzyme deactivation or ultrasonic enzyme deactivation, the plant is one of flowers, tea, citrus and coffee, the addition amount of the complex enzyme preparation is 3% -8% of the mass of the plant residue, and the complex enzyme preparation comprises one or more of tannase, cellulase, pectinase, polyphenol oxidase, protease, xylanase and glycosidase.
In a preferred embodiment, the plant is fresh flower, the addition amount of the complex enzyme preparation is 3% of the mass of the plant residue, and the complex enzyme preparation is mixed enzyme of cellulase, pectinase and protease according to the mass ratio of 1:1: 2.
In a preferred embodiment, the plant is tea, the addition amount of the complex enzyme preparation is 5% of the mass of the plant residue, and the complex enzyme preparation is tannase, cellulase, pectinase, polyphenol oxidase and protease according to a mass ratio of 1: (1-2): 1:2: 2.
In a preferred embodiment, the plant is citrus, the addition amount of the complex enzyme preparation is 6% of the mass of the plant residue, and the complex enzyme preparation is mixed enzyme of cellulase, protease and xylanase according to the mass ratio of 1:2: 2.
In a preferred embodiment, the plants are coffee, the addition amount of the complex enzyme preparation is 7% of the mass of the plant residues, and the complex enzyme preparation is a mixture of cellulase, protease and glycosidase in a mass ratio of 1:2: 1.
The invention has the beneficial effects that:
through once broken and ethyl acetate soak extraction, extract the composition that dissolves in organic solvent in the plant, then pass through the enzymolysis mode to the plant sediment, carry out the broken wall with the plant cell wall, and enzymolysis nutrient composition, again through ethanol extraction, combine the technique of squeezing, fully extract nutrient composition in the plant, then get rid of the extractant through water bath distillation, obtain the plant extract through the concentrated mode of membrane, energy-concerving and environment-protective, effectively improve extraction efficiency, reduce cost, and improve the extract taste through the enzymolysis, guarantee the health through active carbon and pasteurization, the method is simple and convenient, and strong operability, and has popularization and use value.
Drawings
Fig. 1 is a schematic diagram of a method for producing an enzyme-treated plant extract according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1: as shown in fig. 1, the present invention provides a method for preparing an enzyme-treated plant extract, comprising the steps of:
s1, crushing the plants, inactivating original enzymes, adding the plants into an extraction tank, adding ethyl acetate to perform initial extraction of plant components, and performing centrifugal separation and membrane filtration to obtain initial extract and plant residues;
s2, heating the plant residues to remove ethyl acetate extractant, adding the plant residues into a new extraction tank, adding deionized water, stirring uniformly, adding a complex enzyme preparation, carrying out enzymolysis, adding ethanol, carrying out continuous low-temperature extraction, and carrying out centrifugal separation and membrane filtration to obtain enzyme extract and secondary plant residues;
s3, inactivating enzyme of the secondary enzyme extract, adding the primary extract into the secondary enzyme extract for mixing, and removing ethyl acetate and ethanol by heating and distilling in a water bath to obtain a mixed extract;
s4, adsorbing and decoloring the mixed extract by using active carbon, removing the active carbon by centrifugal separation and membrane filtration, sterilizing at high temperature, and concentrating the extract to obtain the plant extract.
In a preferred embodiment, in step S1, the weight ratio of ethyl acetate to plant weight is 3-5:1, the initial extraction temperature is 20-50 ℃, the stirring is carried out while the extraction is carried out, and the extraction time is 1-2 h.
In a preferred embodiment, in step S2, the addition amount of deionized water is 2-3 times of the plant residue, the enzymolysis temperature is 25-40 ℃, the enzymolysis time is 8-12h, the addition amount of ethanol is 30-40% of the mass of the mixed solution, the extraction temperature of the continuous low-temperature extraction is 20-30 ℃, the pressure is 0.30-1.2MPa, the extraction time is 20-50min, the continuous extraction is performed for 3-5 times, the secondary plant residue after the centrifugal separation and the membrane filtration is squeezed, the squeezed filtrate is mixed with the enzyme extract, and the residue is waste residue.
In a preferred embodiment, in step S3, the temperature of the water bath is 80-85 ℃, and the ethyl acetate and ethanol distilled by the water bath are condensed and recovered.
In a preferred embodiment, in step S4, the weight-to-volume (g/ml) ratio of the activated carbon to the mixed extract is 35-8:100, and the sterilization is performed by high-temperature instantaneous sterilization at 125-145 ℃ for 5-10S.
In a preferred embodiment, the enzyme extinguishing method adopts microwave enzyme deactivation or ultrasonic enzyme deactivation, the plant is fresh flower, the addition amount of the complex enzyme preparation is 3% of the mass of the plant residue, and the complex enzyme preparation is mixed enzyme of cellulase, pectinase and protease according to the mass ratio of 1:1: 2.
Example 2: as shown in fig. 1, the present invention provides a method for preparing an enzyme-treated plant extract, comprising the steps of:
s1, crushing the plants, inactivating original enzymes, adding the plants into an extraction tank, adding ethyl acetate to perform initial extraction of plant components, and performing centrifugal separation and membrane filtration to obtain initial extract and plant residues;
s2, heating the plant residues to remove ethyl acetate extractant, adding the plant residues into a new extraction tank, adding deionized water, stirring uniformly, adding a complex enzyme preparation, carrying out enzymolysis, adding ethanol, carrying out continuous low-temperature extraction, and carrying out centrifugal separation and membrane filtration to obtain enzyme extract and secondary plant residues;
s3, inactivating enzyme of the secondary enzyme extract, adding the primary extract into the secondary enzyme extract for mixing, and removing ethyl acetate and ethanol by heating and distilling in a water bath to obtain a mixed extract;
s4, adsorbing and decoloring the mixed extract by using active carbon, removing the active carbon by centrifugal separation and membrane filtration, sterilizing at high temperature, and concentrating the extract to obtain the plant extract.
In a preferred embodiment, in step S1, the weight ratio of ethyl acetate to plant weight is 3-5:1, the initial extraction temperature is 20-50 ℃, the stirring is carried out while the extraction is carried out, and the extraction time is 1-2 h.
In a preferred embodiment, in step S2, the addition amount of deionized water is 2-3 times of the plant residue, the enzymolysis temperature is 25-40 ℃, the enzymolysis time is 8-12h, the addition amount of ethanol is 30-40% of the mass of the mixed solution, the extraction temperature of the continuous low-temperature extraction is 20-30 ℃, the pressure is 0.30-1.2MPa, the extraction time is 20-50min, the continuous extraction is performed for 3-5 times, the secondary plant residue after the centrifugal separation and the membrane filtration is squeezed, the squeezed filtrate is mixed with the enzyme extract, and the residue is waste residue.
In a preferred embodiment, in step S3, the temperature of the water bath is 80-85 ℃, and the ethyl acetate and ethanol distilled by the water bath are condensed and recovered.
In a preferred embodiment, in step S4, the weight-to-volume (g/ml) ratio of the activated carbon to the mixed extract is 35-8:100, and the sterilization is performed by high-temperature instantaneous sterilization at 125-145 ℃ for 5-10S.
In a preferred embodiment, the method for enzyme fire extinguishing adopts microwave enzyme deactivation or ultrasonic enzyme deactivation, the plants are tea, the addition amount of the compound enzyme preparation is 5% of the mass of the plant residues, and the compound enzyme preparation is tannase, cellulase, pectinase, polyphenol oxidase and protease according to the mass ratio of 1: (1-2): 1:2: 2.
Example 3: as shown in fig. 1, the present invention provides a method for preparing an enzyme-treated plant extract, comprising the steps of:
s1, crushing the plants, inactivating original enzymes, adding the plants into an extraction tank, adding ethyl acetate to perform initial extraction of plant components, and performing centrifugal separation and membrane filtration to obtain initial extract and plant residues;
s2, heating the plant residues to remove ethyl acetate extractant, adding the plant residues into a new extraction tank, adding deionized water, stirring uniformly, adding a complex enzyme preparation, carrying out enzymolysis, adding ethanol, carrying out continuous low-temperature extraction, and carrying out centrifugal separation and membrane filtration to obtain enzyme extract and secondary plant residues;
s3, inactivating enzyme of the secondary enzyme extract, adding the primary extract into the secondary enzyme extract for mixing, and removing ethyl acetate and ethanol by heating and distilling in a water bath to obtain a mixed extract;
s4, adsorbing and decoloring the mixed extract by using active carbon, removing the active carbon by centrifugal separation and membrane filtration, sterilizing at high temperature, and concentrating the extract to obtain the plant extract.
In a preferred embodiment, in step S1, the weight ratio of ethyl acetate to plant weight is 3-5:1, the initial extraction temperature is 20-50 ℃, the stirring is carried out while the extraction is carried out, and the extraction time is 1-2 h.
In a preferred embodiment, in step S2, the addition amount of deionized water is 2-3 times of the plant residue, the enzymolysis temperature is 25-40 ℃, the enzymolysis time is 8-12h, the addition amount of ethanol is 30-40% of the mass of the mixed solution, the extraction temperature of the continuous low-temperature extraction is 20-30 ℃, the pressure is 0.30-1.2MPa, the extraction time is 20-50min, the continuous extraction is performed for 3-5 times, the secondary plant residue after the centrifugal separation and the membrane filtration is squeezed, the squeezed filtrate is mixed with the enzyme extract, and the residue is waste residue.
In a preferred embodiment, in step S3, the temperature of the water bath is 80-85 ℃, and the ethyl acetate and ethanol distilled by the water bath are condensed and recovered.
In a preferred embodiment, in step S4, the weight-to-volume (g/ml) ratio of the activated carbon to the mixed extract is 35-8:100, and the sterilization is performed by high-temperature instantaneous sterilization at 125-145 ℃ for 5-10S.
In a preferred embodiment, the enzyme extinguishing method adopts microwave enzyme deactivation or ultrasonic enzyme deactivation, the plants are citrus, the addition amount of the compound enzyme preparation is 6% of the mass of plant residues, and the compound enzyme preparation is mixed enzyme of cellulase, protease and xylanase according to the mass ratio of 1:2: 2.
Example 4: as shown in fig. 1, the present invention provides a method for preparing an enzyme-treated plant extract, comprising the steps of:
s1, crushing the plants, inactivating original enzymes, adding the plants into an extraction tank, adding ethyl acetate to perform initial extraction of plant components, and performing centrifugal separation and membrane filtration to obtain initial extract and plant residues;
s2, heating the plant residues to remove ethyl acetate extractant, adding the plant residues into a new extraction tank, adding deionized water, stirring uniformly, adding a complex enzyme preparation, carrying out enzymolysis, adding ethanol, carrying out continuous low-temperature extraction, and carrying out centrifugal separation and membrane filtration to obtain enzyme extract and secondary plant residues;
s3, inactivating enzyme of the secondary enzyme extract, adding the primary extract into the secondary enzyme extract for mixing, and removing ethyl acetate and ethanol by heating and distilling in a water bath to obtain a mixed extract;
s4, adsorbing and decoloring the mixed extract by using active carbon, removing the active carbon by centrifugal separation and membrane filtration, sterilizing at high temperature, and concentrating the extract to obtain the plant extract.
In a preferred embodiment, in step S1, the weight ratio of ethyl acetate to plant weight is 3-5:1, the initial extraction temperature is 20-50 ℃, the stirring is carried out while the extraction is carried out, and the extraction time is 1-2 h.
In a preferred embodiment, in step S2, the addition amount of deionized water is 2-3 times of the plant residue, the enzymolysis temperature is 25-40 ℃, the enzymolysis time is 8-12h, the addition amount of ethanol is 30-40% of the mass of the mixed solution, the extraction temperature of the continuous low-temperature extraction is 20-30 ℃, the pressure is 0.30-1.2MPa, the extraction time is 20-50min, the continuous extraction is performed for 3-5 times, the secondary plant residue after the centrifugal separation and the membrane filtration is squeezed, the squeezed filtrate is mixed with the enzyme extract, and the residue is waste residue.
In a preferred embodiment, in step S3, the temperature of the water bath is 80-85 ℃, and the ethyl acetate and ethanol distilled by the water bath are condensed and recovered.
In a preferred embodiment, in step S4, the weight-to-volume (g/ml) ratio of the activated carbon to the mixed extract is 35-8:100, and the sterilization is performed by high-temperature instantaneous sterilization at 125-145 ℃ for 5-10S.
In a preferred embodiment, the enzyme extinguishing method adopts microwave enzyme deactivation or ultrasonic enzyme deactivation, the plants are coffee, the addition amount of the compound enzyme preparation is 7% of the mass of the plant residues, and the compound enzyme preparation is a mixture of cellulase, protease and glycosidase in a mass ratio of 1:2: 1.
When the plant nutrient extraction method is used, components dissolved in an organic solvent in a plant are extracted through primary crushing and ethyl acetate soaking extraction, then plant cell walls are broken through enzymolysis of plant residues, nutrient components are subjected to enzymolysis, then the plant cell walls are extracted through ethanol extraction, a squeezing technology is combined, nutrient components in the plant are fully extracted, then an extracting agent is removed through water bath distillation, a plant extract is obtained through a membrane concentration mode, energy is saved, the environment is protected, the extraction efficiency is effectively improved, the cost is reduced, the taste of the extract is improved through enzymolysis, and the health is guaranteed through activated carbon and high-temperature sterilization.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A method for producing an enzyme-treated plant extract, comprising the steps of:
s1, crushing the plants, inactivating original enzymes, adding the plants into an extraction tank, adding ethyl acetate to perform initial extraction of plant components, and performing centrifugal separation and membrane filtration to obtain initial extract and plant residues;
s2, heating the plant residues to remove ethyl acetate extractant, adding the plant residues into a new extraction tank, adding deionized water, stirring uniformly, adding a complex enzyme preparation, carrying out enzymolysis, adding ethanol, carrying out continuous low-temperature extraction, and carrying out centrifugal separation and membrane filtration to obtain enzyme extract and secondary plant residues;
s3, inactivating enzyme of the secondary enzyme extract, adding the primary extract into the secondary enzyme extract for mixing, and removing ethyl acetate and ethanol by heating and distilling in a water bath to obtain a mixed extract;
s4, adsorbing and decoloring the mixed extract by using active carbon, removing the active carbon by centrifugal separation and membrane filtration, sterilizing at high temperature, and concentrating the extract to obtain the plant extract.
2. The method for producing an enzyme-treated plant extract according to claim 1, wherein: in step S1, the weight ratio of ethyl acetate to plant is 3-5:1, the initial extraction temperature is 20-50 deg.C, and the extraction is carried out under stirring for 1-2 h.
3. The method for producing an enzyme-treated plant extract according to claim 1, wherein: in the step S2, the addition amount of deionized water is 2-3 times of that of the plant residues, the enzymolysis temperature is 25-40 ℃, the enzymolysis time is 8-12h, the addition amount of ethanol is 30-40% of the mass of the mixed solution, the extraction temperature of the continuous low-temperature extraction is 20-30 ℃, the pressure is 0.30-1.2MPa, the extraction time is 20-50min, the continuous extraction is performed for 3-5 times, the secondary plant residue after the centrifugal separation and the membrane filtration is squeezed, the squeezed filtrate is mixed with the enzyme extract, and the residue is waste residue.
4. The method for producing an enzyme-treated plant extract according to claim 1, wherein: in step S3, the temperature of the water bath is 80-85 ℃, and ethyl acetate and ethanol distilled in the water bath are condensed and recovered.
5. The method for producing an enzyme-treated plant extract according to claim 1, wherein: in step S4, the weight-volume (g/ml) ratio of the activated carbon to the mixed extract is 35-8:100, and the sterilization adopts high-temperature instantaneous sterilization, wherein the sterilization temperature is 125-145 ℃ and the time is 5-10S.
6. The method for producing an enzyme-treated plant extract according to claim 1, wherein: the method for enzyme extinguishment adopts microwave enzyme inactivation or ultrasonic enzyme inactivation, the plant is one of fresh flowers, tea, citrus and coffee, the addition amount of the complex enzyme preparation is 3% -8% of the mass of the plant residue, and the complex enzyme preparation comprises one or more of tannase, cellulase, pectinase, polyphenol oxidase, protease, xylanase and glycosidase.
7. The method for producing an enzyme-treated plant extract according to claim 6, wherein: the plant is fresh flower, the addition amount of the complex enzyme preparation is 3% of the mass of the plant residue, and the complex enzyme preparation is mixed enzyme of cellulase, pectinase and protease according to the mass ratio of 1:1: 2.
8. The method for producing an enzyme-treated plant extract according to claim 6, wherein: the plant is tea, the addition amount of the complex enzyme preparation is 5% of the mass of the plant residue, and the complex enzyme preparation is prepared from tannase, cellulase, pectinase, polyphenol oxidase and protease according to the mass ratio of 1: (1-2): 1:2: 2.
9. The method for producing an enzyme-treated plant extract according to claim 6, wherein: the plant is citrus, the addition amount of the complex enzyme preparation is 6% of the mass of the plant residue, and the complex enzyme preparation is mixed enzyme of cellulase, protease and xylanase according to the mass ratio of 1:2: 2.
10. The method for producing an enzyme-treated plant extract according to claim 6, wherein: the plant is coffee, the addition amount of the complex enzyme preparation is 7% of the mass of the plant residue, and the complex enzyme preparation is a mixture of cellulase, protease and glycosidase in a mass ratio of 1:2: 1.
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| CN116210826A (en) * | 2023-04-03 | 2023-06-06 | 宁夏厚生记枸杞饮品股份有限公司 | Method for preparing medlar compound clear juice by esterification and enzymolysis and compound beverage thereof |
| CN117448078A (en) * | 2023-12-13 | 2024-01-26 | 广州市浩立生物科技有限公司 | Combined enzymolysis technology and supercritical CO 2 Extracted coffee oil and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116210826A (en) * | 2023-04-03 | 2023-06-06 | 宁夏厚生记枸杞饮品股份有限公司 | Method for preparing medlar compound clear juice by esterification and enzymolysis and compound beverage thereof |
| CN117448078A (en) * | 2023-12-13 | 2024-01-26 | 广州市浩立生物科技有限公司 | Combined enzymolysis technology and supercritical CO 2 Extracted coffee oil and preparation method thereof |
| CN117448078B (en) * | 2023-12-13 | 2024-05-14 | 广州市浩立生物科技有限公司 | Combined enzymolysis technology and supercritical CO2Extracted coffee oil and preparation method thereof |
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