CN114403407A - A kind of concentrated soup base preparation method combining boiling concentration and compound enzymolysis - Google Patents

A kind of concentrated soup base preparation method combining boiling concentration and compound enzymolysis Download PDF

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CN114403407A
CN114403407A CN202210025015.8A CN202210025015A CN114403407A CN 114403407 A CN114403407 A CN 114403407A CN 202210025015 A CN202210025015 A CN 202210025015A CN 114403407 A CN114403407 A CN 114403407A
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chicken
soup
concentrated
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enzymolysis
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张慜
吴江红
刘亚萍
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Jiangnan University
Guangdong Jiahao Foodstuff Co Ltd
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Guangdong Jiahao Foodstuff Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L23/00Soups; Sauces; Preparation or treatment thereof
    • A23L23/10Soup concentrates, e.g. powders or cakes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/45Addition of, or treatment with, microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/50Poultry products, e.g. poultry sausages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/50Poultry products, e.g. poultry sausages
    • A23L13/52Comminuted, emulsified or processed products; Pastes; Reformed or compressed products from poultry meat
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/30Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/30Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
    • A23L5/32Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/51Concentration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

本发明提供一种熬煮浓缩与复合酶解结合的浓缩汤底制备方法,属调味料加工技术领域。主要步骤包括:原材料鸡胸肉的处理、增鲜鸡汤的制备、浓缩、鸡肉酶解液的制备、鸡肉酶解粉的制备、特鲜浓缩鸡汤半成品的调配、特鲜浓缩鸡汤成品的制备等。该发明采用真空低温浓缩技术,保证了产品有效成分不因为加热浓缩而失去功效,最大程度保证了产品风味不变化,采用该方法制备得到的特鲜浓缩鸡汤只需较少的使用量即可达到消费者对鲜味的要求;产品用开水稀释25‑30倍后仍具有较高的鲜味,可作为家庭高汤使用,也可冲汤即食。The invention provides a method for preparing a concentrated soup base which combines boiling, concentration and compound enzymatic hydrolysis, and belongs to the technical field of seasoning processing. The main steps include: treatment of raw chicken breast meat, preparation and concentration of fresh chicken soup, preparation of chicken enzymatic hydrolysis liquid, preparation of chicken enzymatic hydrolysis powder, preparation of semi-finished products of special fresh concentrated chicken soup, preparation of finished special fresh concentrated chicken soup, etc. The invention adopts the vacuum low temperature concentration technology, which ensures that the active ingredients of the product do not lose their efficacy due to heating and concentration, and ensures that the flavor of the product does not change to the greatest extent. Consumers' requirements for umami; the product still has a high umami after being diluted 25-30 times with boiling water, and can be used as a family soup or ready-to-eat.

Description

一种熬煮浓缩与复合酶解结合的浓缩汤底制备方法A kind of concentrated soup base preparation method combining boiling concentration and compound enzymolysis

技术领域technical field

本发明属于调味料加工技术领域,具体涉及一种熬煮浓缩与复合酶解结合的浓缩汤底的制备方法。The invention belongs to the technical field of seasoning processing, and in particular relates to a preparation method of a concentrated soup base combined with boiling concentration and compound enzymatic hydrolysis.

背景技术Background technique

国内外对鸡汤的研究主要集中在鸡汤中的营养、风味、贮藏以及食疗功能等方面。相关研究表明不同品种、性别、部位的鸡或不同的煮制器皿、时间、压力等工艺条件以及预炒、醋泡、酶解等前处理的差异对鸡汤中的营养成分和风味物质的组成和含量都有显著的影响。同时不同的灌装方式、杀菌条件、贮藏温度也影响着鸡汤的品质及货架期。在食品中添加鲜味剂,可增加食品的一些风味特征,如持续性、口感性、温和感和浓厚感等。目前我国批准的鲜味剂有谷氨酸钠、呈味核苷酸钠、琥珀酸二钠及动植物蛋白水解酶等。The research on chicken soup at home and abroad mainly focuses on the nutrition, flavor, storage and therapeutic function of chicken soup. Relevant studies have shown that different breeds, genders, parts of chickens or different cooking utensils, time, pressure and other process conditions, as well as pre-frying, vinegar soaking, enzymatic hydrolysis and other pretreatment differences have an impact on the composition and flavor of nutrients and flavors in chicken soup. content has a significant effect. At the same time, different filling methods, sterilization conditions and storage temperature also affect the quality and shelf life of chicken soup. Adding umami agents to food can increase some flavor characteristics of food, such as persistence, taste, mildness and thickness. At present, the umami agents approved in my country include sodium glutamate, sodium nucleotide sodium, disodium succinate, and animal and plant proteolytic enzymes.

目前,国内关于浓缩鸡汤的研究主要集中于开发不同口味及功效的浓缩鸡汤的技术配方与工艺的研究。如刘孟建等(专利申请号CN201410785901.6)公开了一种天麻乌鸡汤药膳配方及其制备方法。该发明通过水煎当归、杜仲及石决明,得浓缩汁;然后将去内脏的乌鸡放入上述浓缩汁中,并加入重量份的天麻、大枣、枸杞、炒枣仁,然后加入水炖煮,加入食盐,即得天麻乌鸡汤药膳,该药膳具有壮骨强筋、熄风定惊功效。如陈悦娇等(专利申请号CN201610546015.7)具体公开了一种即溶蛹虫草鸡汤及其制备方法。将鸡块以及蛹虫草加入水中进行煮制,分离鸡肉和鸡汤,并将鸡汤进行浓缩,得浓缩鸡汤,在浓缩鸡汤中加入添加物进行吸附,干燥后即得所述的即溶蛹虫草鸡汤。由该方法制备得到的即溶蛹虫草鸡汤与原汤相比虫草素、虫草多糖、氨基酸态氮的保留率高。At present, domestic research on concentrated chicken soup mainly focuses on the research on the technical formula and process of developing concentrated chicken soup with different tastes and functions. For example, Liu Mengjian et al. (patent application number CN201410785901.6) disclose a medicinal diet formula of Tianma black chicken soup and a preparation method thereof. In the invention, the concentrated juice is obtained by decoction of Angelica sinensis, Eucommia ulmoides and Cassia dahliae; then the black-bone chicken that has been removed from the internal organs is put into the above-mentioned concentrated juice, and the Gastrodia elata, jujube, wolfberry and fried jujube kernels in parts by weight are added, and then water is added and stewed Boil it and add salt to get Tianma black chicken soup medicated diet, which has the effect of strengthening bones and tendons, suppressing wind and calming shock. For example, Chen Yuejiao et al. (patent application number CN201610546015.7) specifically disclose an instant chicken soup with Cordyceps militaris and a preparation method thereof. The chicken nuggets and Cordyceps militaris are added into water for cooking, the chicken and the chicken broth are separated, and the chicken broth is concentrated to obtain concentrated chicken broth, and additives are added to the concentrated chicken broth for adsorption, and after drying, the instant-dissolving Cordyceps militaris chicken broth is obtained. Compared with the original soup, the instant-dissolving cordyceps militaris chicken soup prepared by the method has higher retention rates of cordycepin, cordyceps polysaccharide and amino acid nitrogen.

朱映融等(专利申请号CN201911231884.0)公开了一种浓缩鸡汤的制备方法。主要是在半小时内,对180天骨龄的散养鸡进行屠宰;进行去酸处理,将鸡和水放置容器内,在100℃、50Pa下对鸡进行萃取,萃取5小时,获得液体;对液体进行去脂、去胆固醇处理后,获得浓缩鸡汤。该方法由于高温萃取时间过长,容易导致鸡汤风味变差,且鲜味成分含量降低。本发明采用真空低温浓缩技术,保证了产品有效成分不因为加热浓缩而失去功效,最大程度保证了产品风味不变化,采用该方法制备得到的特鲜浓缩鸡汤只需较少的使用量即可达到消费者对鲜味的要求。Zhu Yingrong et al. (Patent Application No. CN201911231884.0) disclose a preparation method of concentrated chicken soup. Mainly, slaughter the 180-day-old free-range chickens within half an hour; perform deacidification, place the chickens and water in a container, and extract the chickens at 100 ° C and 50 Pa for 5 hours to obtain liquid; After the liquid is de-fat and cholesterol-removed, concentrated chicken soup is obtained. Due to the long extraction time at high temperature in this method, the flavor of chicken soup is easily deteriorated, and the content of umami components is reduced. The invention adopts the vacuum low-temperature concentration technology, which ensures that the effective components of the product do not lose their efficacy due to heating and concentration, and ensures that the flavor of the product does not change to the greatest extent. Consumer demand for umami.

刘学荣等(专利申请号CN201410467378.2)公开了一种便携鸡汤的加工方法。利用传统的鸡汤制作方法和现代生物技术工艺相结合的加工操作流程,将优选冰鲜鸡骨、净化水、新鲜的大葱、生姜和天然香辛料加入浸提专用夹层高压罐进行浸提、静置、浓缩、调味而成的浓缩型鸡汤,分装成一次性小盒和包装袋,方便家庭和出行、旅游食用,可根据个人口味加入20至30倍的温水或热水直接饮用,也可作家庭炒菜、下面的营养鸡汤。但该发明不能使便携鸡汤在20-30倍稀释后依旧保持原有的鸡汤鲜味,且各方面滋味可能变淡。本发明能够使鸡汤在25-30倍稀释后,鲜味可与传统鸡汤鲜味相媲美,且风味变化不大,甚至有所改善。Liu Xuerong et al. (Patent Application No. CN201410467378.2) disclosed a processing method of portable chicken soup. Using the processing operation process combining traditional chicken soup production methods and modern biotechnological processes, the preferred chilled chicken bones, purified water, fresh green onions, ginger and natural spices are added to the special interlayer high pressure tank for leaching for leaching, standing, and Concentrated and seasoned concentrated chicken soup, divided into disposable small boxes and packaging bags, convenient for family and travel, tourism consumption, can be added 20 to 30 times of warm water or hot water according to personal taste to drink directly, can also be used for family Stir-fry, nutritious chicken soup below. However, the invention cannot make the portable chicken soup still maintain the original umami taste of the chicken soup after being diluted 20-30 times, and the taste may become weaker in all aspects. The invention can make the umami of chicken soup after 25-30 times dilution, and the umami taste of traditional chicken soup is comparable to that of traditional chicken soup, and the flavor changes little or even improves.

张立彦等(专利申请号CN201310373607.X)公开了一种鸡骨高汤及其制备方法。将鸡骨架清洗后,切割成鸡骨块;把鸡骨块放入水中加热熬煮,得到鸡骨汤;将鸡骨汤过滤,滤出骨块,所得鸡骨汤汁冷冻后,用滤布分离出鸡油及鸡骨汤汁;将鸡骨汤汁真空浓缩,得到浓缩鸡骨汤,加入蔗糖酯、黄原胶、卡拉胶、β-环糊精、食盐、白砂糖和鲜味剂,充分溶解;往鸡油中加入分子蒸馏单甘酯,充分溶解;与上述溶解后浓缩鸡汤混合均质;将均质后所得的鸡骨汤真空脱气,分装,高压杀菌,冷却至室温,即得鸡骨高汤。此发明流程简单、成品产出时间短,便于工厂规范化操作;所得鸡骨高汤色泽乳白、状态稳定、鲜味醇厚、回味绵长、营养丰富,不含防腐剂。但这种鸡骨高汤在较高的稀释倍数下鲜度下降。Zhang Liyan et al. (Patent Application No. CN201310373607.X) disclose a chicken bone broth and a preparation method thereof. After cleaning the chicken skeleton, cut it into chicken bone pieces; heat and boil the chicken bone pieces in water to obtain chicken bone soup; filter the chicken bone soup, filter out the bone pieces, and after the obtained chicken bone soup is frozen, use a filter cloth Separate chicken oil and chicken bone broth; vacuum concentrate chicken bone broth to obtain concentrated chicken bone broth, add sucrose ester, xanthan gum, carrageenan, β-cyclodextrin, table salt, white sugar and umami agent, Fully dissolving; adding molecularly distilled monoglyceride to the chicken oil, fully dissolving; mixing and homogenizing with the above-mentioned dissolving concentrated chicken soup; vacuum degassing the chicken bone soup obtained after the homogenization, packaging, autoclaving, cooling to room temperature, That's the chicken bone broth. The invention has simple process, short finished product output time, and is convenient for standardized operation of factories; the obtained chicken bone broth is milky white in color, stable in state, mellow in flavor, long in aftertaste, rich in nutrition and free of preservatives. However, the freshness of this chicken bone broth decreased at higher dilution ratios.

夏杨毅等(专利申请号CN201610946666.5)公开了一种富含鲜味肽营养鸡汤及其制备方法,鸡胸肉解冻后洗净,搅碎、加水,搅拌成料液;加入复合蛋白酶进行适度酶解,离心分离上清液进行美拉德反应。采用适度酶解技术,提高鸡肉蛋白溶出率,鸡肉酶解液富含氨基酸及小分子多肽,发生美拉德反应后的热反应鸡汤在保持传统鸡汤原香和美味的同时,其中游离氨基酸达到传统鸡汤的20多倍,小分子肽类等大大增加,更容易被人体消化吸收。本发明不仅富含鲜味肽,还额外添加了强鲜味剂—干贝素,干贝素和鸡肉酶解液可共同提鲜,并改善鸡汤滋味,增强鸡汤特有风味。Xia Yangyi et al. (Patent Application No. CN201610946666.5) disclose a nutritious chicken soup rich in umami peptides and a preparation method thereof. The chicken breast is thawed, washed, crushed, added with water, and stirred into a feed liquid; compound protease is added to moderately After enzymatic hydrolysis, the supernatant was centrifuged for Maillard reaction. Using moderate enzymatic hydrolysis technology to improve the dissolution rate of chicken protein, the chicken enzymatic hydrolysate is rich in amino acids and small molecular polypeptides. It is more than 20 times that of chicken soup, and the small molecule peptides are greatly increased, making it easier to be digested and absorbed by the human body. The present invention is not only rich in umami peptides, but also additionally adds a strong umami agent - dried scallops. The dried scallops and chicken enzymolysis liquid can jointly improve the freshness, improve the taste of chicken soup, and enhance the unique flavor of chicken soup.

本发明将鸡汤、鸡肉酶解液和干贝素复配处理,采用旋转蒸发浓缩方式,以电子舌和感官评价数据为指标,以评价鸡汤鲜味及口感变化,工艺简单,添加剂含量少,鲜味较高,风味较好,具有较好的口感,具有较高的蛋白质和氨基酸含量,为浓缩汤底的工业化、规模化和标准化生产,以及适应餐饮业连锁经营标准化的需求提供理论依据指导。In the invention, the chicken soup, the chicken enzymolysis solution and the scallops are compounded and processed, and the rotary evaporation and concentration method is adopted, and the electronic tongue and sensory evaluation data are used as indicators to evaluate the freshness and taste changes of the chicken soup. Higher, better flavor, better taste, higher protein and amino acid content, provide theoretical basis and guidance for the industrialization, large-scale and standardized production of concentrated soup base, and to meet the needs of the standardization of chain operations in the catering industry.

发明内容SUMMARY OF THE INVENTION

本发明的目的是提供一种熬煮浓缩与复合酶解结合制备经25-30倍高度稀释后鸡汤鲜味仍保持传统鸡汤原有鲜味的特鲜浓缩汤底的制备方法,该发明仅添加两种特鲜成分进行复配即可达到特鲜的状态。The purpose of this invention is to provide a kind of preparation method that boils and concentrates and compound enzymolysis combine to prepare the special fresh concentrated soup base which still retains the original umami taste of traditional chicken soup after 25-30 times of high dilution, this invention only adds The two special fresh ingredients can be combined to achieve the special fresh state.

本发明的技术方案:Technical scheme of the present invention:

一种熬煮浓缩与复合酶解结合的浓缩汤底制备方法,其特征在于,主要步骤如下:A method for preparing a concentrated soup base combining boiling, concentration and compound enzymolysis, characterized in that the main steps are as follows:

(1)原材料的处理:鸡胸肉A用清水洗净,切块、焯水去浮沫、清水再次冲洗并沥干得鸡胸肉块,用于鸡汤的熬制;鸡胸肉B洗净、切条,于绞肉机中搅碎,获得鸡肉糜,用于鸡肉酶解液的制备;(1) Processing of raw materials: Wash chicken breast A with clean water, cut into pieces, blanch with water to remove scum, rinse with water again and drain to obtain chicken breast pieces, which are used for cooking chicken soup; chicken breast B is washed and cut into strips , crushed in a meat grinder to obtain minced chicken, which is used for the preparation of chicken enzymatic hydrolyzate;

(2)增鲜鸡汤的制备:制备小苏打溶液,加于鸡胸肉块中,用超声预处理15min,并于营养炖锅中炖制90min,得鸡汤粗品;(2) preparation of fresh chicken soup: prepare baking soda solution, add in chicken breast pieces, pre-treat with ultrasonic for 15min, and simmer for 90min in nutrition stew pot to obtain crude chicken soup;

(3)旋转蒸发浓缩:鸡汤粗品经200目滤网分离得汤液和鸡肉,将汤液通过真空低温浓缩,得浓缩鸡汤半成品;(3) Rotary evaporation concentration: the chicken soup crude product is separated through 200 mesh strainers to obtain soup liquid and chicken, and the soup liquid is concentrated by vacuum low temperature to obtain the semi-finished product of concentrated chicken soup;

(4)鸡肉酶解液的制备:鸡肉糜加入去离子水,红外加热预处理,用风味蛋白酶水解,然后加入中性蛋白酶水解得鸡肉酶解液;(4) Preparation of chicken enzymatic hydrolysis solution: chicken minced chicken is added with deionized water, pretreated by infrared heating, hydrolyzed with flavor protease, and then hydrolyzed by neutral protease to obtain chicken enzymatic hydrolysis solution;

(5)鸡肉酶解粉的制备:将鸡肉酶解液加热至90℃,20分钟以灭活所述中性蛋白酶和风味蛋白酶,将鸡肉酶解液离心10分钟,取上清液冻干成粉末,得鸡肉酶解粉;(5) Preparation of chicken enzymolysis powder: the chicken enzymolysis solution was heated to 90° C. for 20 minutes to inactivate the neutral protease and flavor protease, the chicken enzymolysis solution was centrifuged for 10 minutes, and the supernatant was lyophilized into a Powder, get chicken enzymolysis powder;

(6)特鲜浓缩鸡汤半成品的调配:将鸡肉酶解粉、干贝素和食盐加入浓缩鸡汤半成品中进行调配,保温,得特鲜浓缩鸡汤半成品;(6) The preparation of the semi-finished product of special fresh concentrated chicken soup: the enzymolysis powder of chicken, dried scallops and salt are added to the semi-finished product of concentrated chicken soup for deployment, heat preservation, and the semi-finished product of special fresh concentrated chicken soup is obtained;

(7)特鲜浓缩鸡汤成品的制备:添加调味物质至特鲜浓缩鸡汤半成品中进行调配,保温,得特鲜浓缩鸡汤成品。(7) Preparation of the finished product of the extra-fresh concentrated chicken soup: adding seasoning substances to the semi-finished product of the extra-fresh concentrated chicken soup for deployment, and keeping the temperature to obtain the finished product of the extra-fresh concentrated chicken soup.

步骤(1)所述新鲜鸡胸肉A切成2cm×2cm×2cm规格的鸡胸肉块,焯水2min去浮沫,绞肉机搅打鸡肉时间为1min。The fresh chicken breast A in step (1) is cut into chicken breast pieces of 2cm×2cm×2cm, blanched in water for 2 minutes to remove foam, and the meat grinder whips the chicken for 1 minute.

步骤(2)向0.1%的小苏打溶液中按料液比1:2的比例加入50g鸡胸肉块,所述超声处理条件为45KHZ,120W。Step (2) Add 50 g of chicken breast meat to the 0.1% baking soda solution in a ratio of 1:2 to a material-to-liquid ratio, and the ultrasonic treatment conditions are 45KHZ, 120W.

步骤(3)所述汤液真空低温浓缩的条件为-0.09~-0.1MPa的压力,55℃下旋转蒸发浓缩至固形物含量为20%-30%。The conditions for the vacuum low-temperature concentration of the soup in step (3) are the pressure of -0.09 to -0.1 MPa, and the solid content is concentrated by rotary evaporation at 55° C. to 20%-30%.

步骤(4)所述鸡肉糜加入去离子水为1:2.5,w:w,底物浓度28.57%,在35℃下红外加热处理30min,处理完后用鸡肉重量的0.1%的风味蛋白酶在54℃水浴水解1.5h,然后加入鸡肉重量的0.25%的中性蛋白酶在54℃下水浴水解2.5小时。Step (4) Add deionized water to the chicken minced 1:2.5, w:w, substrate concentration 28.57%, infrared heat treatment at 35 ° C for 30min, after the treatment, use 0.1% of the chicken weight flavor protease at 54 ℃ water bath hydrolysis for 1.5h, then add 0.25% of the chicken weight neutral protease and hydrolyze in water bath at 54 ℃ for 2.5 hours.

所述步骤(5)将鸡肉酶解液在50mL离心管中以3922g相对离心力在4℃下离心10分钟,收集上清液并冻干成粉末。In the step (5), the chicken enzymatic hydrolyzed solution was centrifuged in a 50 mL centrifuge tube with a relative centrifugal force of 3922 g at 4° C. for 10 minutes, and the supernatant was collected and lyophilized into powder.

所述步骤(6)中按照浓缩鸡汤半成品重量百分比,加入4-6%食用盐、0.5-2%干贝素和2%鸡肉酶解粉;调配温度81-85℃,保温时间30-50分钟。In the step (6), add 4-6% edible salt, 0.5-2% scallops and 2% chicken enzymolysis powder according to the weight percentage of the semi-finished product of concentrated chicken soup;

所述步骤(7)中调味物质中各原料按照重量百分比包括:5%明胶、2%蔗糖;调配温度81-85℃,保温时间30-50分钟。In the step (7), each raw material in the seasoning substance includes, according to weight percentage: 5% gelatin, 2% sucrose; the preparation temperature is 81-85° C., and the holding time is 30-50 minutes.

本发明的有益效果:Beneficial effects of the present invention:

(1)超声处理可以通过空化作用和机械效应产生的静电力来改变蛋白质的结构,从而产生大量的鲜味氨基酸,促进鲜味核苷酸的生成,并缩短获得较鲜鸡汤的熬煮时间。(1) Ultrasonic treatment can change the structure of protein through the electrostatic force generated by cavitation and mechanical effect, thereby producing a large amount of umami amino acids, promoting the generation of umami nucleotides, and shortening the cooking time for obtaining fresher chicken soup .

(2)酶解前的红外加热预处理能使蛋白热变性,从而促进鲜味氨基酸和鲜味核苷酸的释放,利用安全的物理场技术预处理,改善了鸡肉酶解液的风味。(2) Infrared heating pretreatment before enzymolysis can thermally denature the protein, thereby promoting the release of umami amino acids and umami nucleotides. Pretreatment with safe physical field technology improves the flavor of the chicken enzymolysis solution.

(3)复合酶解过程把鸡肉中不溶蛋白水解成小分子的多肽物质,提高了产品食用后人体吸收率,实现产品的营养价值提升。同时,采用复合酶解技术处理能获得风味较好的酶解液。(3) The complex enzymatic hydrolysis process hydrolyzes the insoluble protein in chicken into small molecular polypeptide substances, which improves the absorption rate of the human body after the product is eaten, and realizes the improvement of the nutritional value of the product. At the same time, the enzymatic hydrolyzate with better flavor can be obtained by using compound enzymolysis technology.

(4)干贝素和鸡肉酶解粉等鲜味剂的添加能起到增鲜效果,增鲜的同时咸味也得到了增加,可以减少食盐的添加,符合目前人们所提倡的低钠盐食品,只需较少的使用量即可达到消费者对鲜味的要求。(4) The addition of umami agents such as dried scallops and chicken enzymolysis powder can increase the freshness, and the salty taste is also increased while increasing the freshness. , only a small amount of use can meet the consumer's requirements for umami.

(5)采用真空低温浓缩技术,保证了产品有效成分不因为加热浓缩而失去功效,最大程度保证了产品风味不变化,使产品口感风味达到人们喜好和嗜好。另外,经过浓缩,减少了鸡汤重量及流通过程的运输成本。(5) The vacuum low-temperature concentration technology is adopted to ensure that the active ingredients of the product will not lose their efficacy due to heating and concentration, and to the greatest extent, the product flavor will not change, so that the taste and flavor of the product will meet people's preferences and preferences. In addition, after concentration, the weight of chicken soup and the transportation cost of the circulation process are reduced.

(6)产品用开水稀释25-30倍后仍具有较高的鲜味,可作为家庭高汤使用,也可冲汤即食,为人们提供方便性。(6) After the product is diluted 25-30 times with boiling water, it still has a high umami taste. It can be used as a family soup, and it can also be brewed and eaten immediately, providing convenience for people.

具体实施方式Detailed ways

为了使本发明的目的、技术方案及优点更加清楚明白,以下结合实施例,对本发明进行进一步详细说明。应当理解,此处所描述的具体实施例仅用以解释本发明,并不用于限定本发明。In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

下面将结合具体实施例对本发明的技术方案进行进一步的说明。The technical solutions of the present invention will be further described below with reference to specific embodiments.

实施例:Example:

原料要求:采用经检验合格的冰鲜鸡胸肉为原料、食盐、食品级小苏打、干贝素、蔗糖、食品级明胶。Raw material requirements: Use the tested and qualified chilled chicken breast as raw materials, salt, food-grade baking soda, scallops, sucrose, food-grade gelatin.

鸡肉汤原料的预处理:鸡胸肉先用清水洗干净,去除鸡胸肉多余的结缔组织,切成2xcmx2cmx2cm的鸡肉块,加入少量料酒腌制5min,去腥后加水冲洗,再冷水下锅焯水2min,捞出沥干。Pretreatment of chicken soup raw materials: Wash the chicken breast with clean water first, remove the excess connective tissue of the chicken breast, cut into chicken pieces of 2xcmx2cmx2cm, add a small amount of cooking wine and marinate for 5 minutes, rinse with water after removing the fishy smell, and then blanch in cold water for 2 minutes , remove and drain.

增鲜浓缩鸡肉汤的制备:添加沥干的鸡胸肉于电炖锅内,加入2倍鸡胸肉重量的0.1%的小苏打溶液,并用记号笔标记水位线,将超声探头深入液面以下2cm处,以45KHZ120W强度超声处理15min;设定煲汤时间为1.5h,期间多次向电炖锅中加0.1%小苏打溶液至水位线,最后制得鸡汤粗品。Preparation of fresh-enhancing concentrated chicken soup: add drained chicken breasts to the electric stew pot, add 0.1% baking soda solution 2 times the weight of chicken breasts, mark the water level line with a marker, and place the ultrasonic probe 2cm below the liquid surface , ultrasonically treated at 45KHZ120W intensity for 15min; set the soup time as 1.5h, add 0.1% baking soda solution to the water level to the electric stewing pot several times during this period, and finally obtain the crude chicken soup.

利用200目的滤网分离鸡汤粗品分离出汤液和鸡肉,将汤液部分放入冰箱冷藏过夜,次日将浮在汤液表面的油脂去除,剩余的清汤采用在-0.09~-0.1MPa的压力,55℃下旋转蒸发浓缩至固形物含量为20%-30%。Use a 200-mesh strainer to separate the crude chicken soup to separate the soup and chicken, put the soup part into the refrigerator to refrigerate overnight, remove the oil floating on the surface of the soup the next day, and use the pressure of -0.09~-0.1MPa for the remaining clear soup. , concentrated by rotary evaporation at 55°C to a solid content of 20%-30%.

鸡肉酶解粉的制备:取部分冰鲜鸡胸肉剁碎,以鸡肉的总重为计,添加总重2.5倍的去离子水,35℃下红外加热处理30min,加入鸡肉总重0.1%的风味蛋白酶,搅拌,放置54℃恒温水浴锅,进行第一次酶解,90分钟后,得酶解液1;继续加入中性蛋白酶至酶解液1中,加入量为原料鸡胸的总重的0.25%,温度控制在54℃,进行二次酶解,酶解时间2.5小时,得酶解液2。二次酶解完成后,加热酶解液2到90℃,恒温20分钟,使得中性蛋白酶和风味蛋白酶进行灭活。灭酶后的酶解液在50mL离心管中以3922g相对离心力(RCF)在4℃下离心10分钟,取上清液进行冻干操作,制得鸡肉酶解粉。Preparation of chicken enzymatic hydrolyzed powder: take part of the chilled chicken breast and mince it, based on the total weight of the chicken, add 2.5 times the total weight of deionized water, heat at 35°C for 30 minutes, and add 0.1% of the total weight of the chicken. Protease, stir, place in a constant temperature water bath at 54°C, and perform the first enzymolysis. After 90 minutes, get the enzymolysis solution 1; continue to add neutral protease to the enzymolysis solution 1, and the addition amount is 0.25 of the total weight of the raw chicken breast. %, the temperature was controlled at 54°C, and the second enzymatic hydrolysis was carried out, and the enzymatic hydrolysis time was 2.5 hours to obtain the enzymatic hydrolysis solution 2. After the secondary enzymatic hydrolysis is completed, the enzymatic hydrolysis solution is heated at 2 to 90°C for 20 minutes to inactivate neutral protease and flavor protease. The enzymatic hydrolysate after inactivation of the enzyme was centrifuged at 4°C for 10 minutes at 3922 g relative centrifugal force (RCF) in a 50 mL centrifuge tube, and the supernatant was taken for freeze-drying operation to obtain chicken enzymatic hydrolyzed powder.

特鲜浓缩鸡汤半成品的调配:以称浓缩物的重量计,添加调味物质,各调味物质按照质量百分比包括:4-6%食用盐、0.5-2%干贝素、2%鸡肉酶解粉搅拌,加热升温到调配温度81℃开始计时,恒温温度控制在81-85℃,保温时间30-50分钟。The preparation of semi-finished products of special fresh concentrated chicken soup: based on the weight of the weighed concentrate, add seasoning substances, each seasoning substance according to mass percentage includes: 4-6% edible salt, 0.5-2% dried scallops, 2% chicken enzymatic hydrolyzed powder stirring, Heating and raising the temperature to the preparation temperature of 81°C starts timing, the constant temperature is controlled at 81-85°C, and the holding time is 30-50 minutes.

特鲜浓缩鸡汤成品的制备:向调配好的特鲜浓缩鸡汤半成品中加入5%明胶、2%蔗糖,搅拌,调配温度81-85℃,保温时间30-50分钟,得到特鲜浓缩鸡汤。取1g特鲜浓缩鸡汤成品加入24g清水于锅中,加热15min制得醇厚的25倍稀释鸡汤。另取1g特鲜浓缩鸡汤成品加入29g清水于锅中,加热15min制得醇厚的30倍稀释鸡汤。Preparation of special fresh concentrated chicken soup finished product: add 5% gelatin and 2% sucrose to the prepared semi-finished special fresh concentrated chicken soup, stir, adjust the temperature at 81-85°C, and keep the temperature for 30-50 minutes to obtain the special fresh concentrated chicken soup. Take 1g of the special fresh concentrated chicken soup product, add 24g of clear water to the pot, and heat for 15min to obtain a mellow 25-fold diluted chicken soup. In addition, 1g of the special fresh concentrated chicken soup product was added to 29g of water in a pot, and heated for 15min to obtain a mellow 30-fold diluted chicken soup.

对照组1:鸡胸肉先用清水洗干净,去除鸡胸肉多余的结缔组织,切成2xcmx2cmx2cm的鸡肉块,加入少量料酒腌制5min,去腥后加水冲洗,再冷水下锅焯水2min,捞出沥干。不做任何预处理,添加沥干的鸡胸肉于电炖锅内,加入2倍鸡胸肉重量的纯净水及鸡胸肉重量1%的食用盐,并用记号笔标记水位线,设定煲汤时间为1.5h,期间多次向电炖锅中加入纯净水至水位线。将所熬好的鸡汤收集起来放入不锈钢锅中,盖上锅盖,采用常压小火蒸发浓缩技术,浓缩至固形物含量在20%-30%,得到对照浓缩鸡汤。取1g对照浓缩鸡汤加入24g清水于锅中,加热15min制得对照组鸡汤1。Control group 1: Wash the chicken breast with clean water first, remove the excess connective tissue of the chicken breast, cut into chicken pieces of 2xcmx2cmx2cm, add a small amount of cooking wine to marinate for 5 minutes, remove the fishy smell, add water to rinse, then blanch in cold water for 2 minutes, remove Drain. Without any pretreatment, add the drained chicken breast to the electric stew pot, add purified water 2 times the weight of the chicken breast and 1% salt of the chicken breast weight, mark the water level line with a marker, and set the soup cooking time to 1.5 h, during this period, add pure water to the electric cooker to the water level line several times. The boiled chicken soup is collected and put into a stainless steel pot, covered with a lid, and concentrated to a solid content of 20%-30% by using the normal pressure and low fire evaporation concentration technology to obtain a control concentrated chicken soup. Take 1 g of the control concentrated chicken soup, add 24 g of clear water to the pot, and heat for 15 min to prepare the control chicken soup 1.

对照组2:Control group 2:

鸡胸肉先用清水洗干净,去除鸡胸肉多余的结缔组织,切成2cm×2cm×2cm的鸡肉块,加入少量料酒腌制5min,去腥后加水冲洗,再冷水下锅焯水2min,捞出沥干。不做任何预处理,添加沥干的鸡胸肉于电炖锅内,加入2倍鸡胸肉重量的纯净水,并用记号笔标记水位线,设定煲汤时间为1.5h,期间多次向电炖锅中加入纯净水至水位线。将所熬好的鸡汤收集起来放入不锈钢锅中,盖上锅盖,采用常压小火蒸发浓缩技术,浓缩至固形物含量在20%-30%,得到浓缩鸡汤。Wash the chicken breast with clean water first, remove the excess connective tissue of the chicken breast, cut into chicken pieces of 2cm x 2cm x 2cm, add a small amount of cooking wine and marinate for 5 minutes, rinse with water after removing the fishy smell, then blanch in cold water for 2 minutes, remove Drain. Without any pretreatment, add the drained chicken breast to the electric stew pot, add purified water twice the weight of the chicken breast, mark the water level line with a marker, set the soup cooking time to 1.5h, and pour the water to the electric stew pot several times during this period. Add purified water to the water level. The boiled chicken soup is collected and put into a stainless steel pot, covered with a lid, and concentrated to a solid content of 20%-30% by using the normal pressure and low fire evaporation concentration technology to obtain concentrated chicken soup.

鸡肉酶解粉的制备:取部分冰鲜鸡胸肉剁碎,以鸡肉的总重为计,添加总重2.5倍的去离子水,35℃下红外加热处理30min,加入中性蛋白酶至酶解液中,加入量为原料鸡胸的总重的0.25%,温度控制在54℃,酶解时间2.5小时。将酶解好的酶解液加热到90℃,恒温20分钟,使得中性蛋白酶灭活。灭酶后的酶解液在50mL离心管中以3922g相对离心力(RCF)在4℃下离心10分钟,取上清液进行冻干操作,制得鸡肉酶解粉。Preparation of chicken enzymolysis powder: take part of the chilled chicken breast and chop it up, take the total weight of the chicken as the total weight, add deionized water 2.5 times the total weight, heat at 35°C for 30min by infrared heating, add neutral protease to the enzymolysis solution , the addition amount was 0.25% of the total weight of the raw chicken breast, the temperature was controlled at 54° C., and the enzymatic hydrolysis time was 2.5 hours. The enzymatic hydrolyzed solution was heated to 90°C and kept at a constant temperature for 20 minutes to inactivate the neutral protease. The enzymatic hydrolysate after inactivation of the enzyme was centrifuged at 4°C for 10 minutes at 3922 g relative centrifugal force (RCF) in a 50 mL centrifuge tube, and the supernatant was taken for freeze-drying operation to obtain chicken enzymatic hydrolyzed powder.

浓缩鸡汤的调配:以称浓缩物的重量计,添加调味物质,各调味物质按照质量百分比包括:4-6%食用盐、2%鸡肉酶解粉,搅拌,加热升温到调配温度81℃开始计时,恒温温度控制在81-85℃,保温时间30-50分钟,制得对照浓缩鸡汤。取1g鸡汤浓缩液加入24g清水于锅中,加热15min制得对照组鸡汤2。Preparation of concentrated chicken soup: add seasoning substances based on the weight of the concentrate, each seasoning substance includes: 4-6% edible salt, 2% chicken enzymatic hydrolyzed powder, stir, heat and heat up to the preparation temperature of 81 ° C to start timing , the constant temperature is controlled at 81-85 DEG C, and the holding time is 30-50 minutes to prepare the control concentrated chicken soup. Take 1g of chicken soup concentrate and add 24g of clear water to the pot, and heat for 15min to prepare control group chicken soup 2.

试验1:电子舌测试Test 1: Electronic Tongue Test

将本发明稀释后的鸡汤和对照组的鸡汤分别存放于冰箱冷藏过夜,次日将鸡汤去除并用200目漏斗过滤掉油脂,所得滤液分别取3组平行样,进行电子舌滋味分析,发现该发明下的鸡汤经过25-30倍高度稀释后的滋味与对照组汤料相差不大。鲜味比对照组略高,咸味明显增高,具体检测结果见表1。The chicken soup after the dilution of the present invention and the chicken soup of the control group are respectively stored in a refrigerator and refrigerated overnight, the chicken soup is removed the next day and the grease is filtered out with a 200-mesh funnel. The taste of the chicken soup after being diluted 25-30 times is not much different from that of the control soup. The umami taste was slightly higher than that of the control group, and the salty taste was significantly increased. The specific test results are shown in Table 1.

表1实施例中制得的特鲜浓缩鸡汤及其对照组的电子舌检测结果The electronic tongue detection result of the special fresh concentrated chicken soup prepared in the embodiment of table 1 and its control group

Figure BDA0003462658370000081
Figure BDA0003462658370000081

试验2:感官评价Test 2: Sensory Evaluation

选取5名有丰富经验的感官评价人员组成评价小组,这些人在感官评价前经过了2周的训练。感官评价内容包括鸡汤的色泽、香味、口感及鸡汤状态。将实施例中经过25倍和30倍稀释后的鸡汤与对照组中的鸡汤于水浴锅中均保持恒温45℃,让感官评价小组人员依次评价,最终的感官评价结果见表2。评价得出实施例中经过25倍稀释后的鸡汤较对照组有更为浓郁的肉风味,鲜味更为突出,颜色较黄,且回味更加持久。Five experienced sensory evaluators were selected to form an evaluation team, and these people had undergone 2 weeks of training before sensory evaluation. The sensory evaluation contents include the color, aroma, taste and state of the chicken soup. The chicken soup diluted by 25 times and 30 times in the example and the chicken soup in the control group were kept at a constant temperature of 45°C in a water bath, and the sensory evaluation team members were evaluated in turn. The final sensory evaluation results are shown in Table 2. The evaluation shows that the chicken soup diluted by 25 times in the example has a stronger meat flavor, more prominent umami, yellower color and longer aftertaste than the control group.

表2实施例中制得的特鲜浓缩鸡汤及其对照组的感官评价The sensory evaluation of the special fresh concentrated chicken soup prepared in the embodiment of table 2 and its control group

Figure BDA0003462658370000091
Figure BDA0003462658370000091

Claims (8)

1. A preparation method of concentrated soup base combining boiling concentration and compound enzymolysis is characterized by mainly comprising the following steps:
(1) treatment of raw materials: cleaning chicken breast A with clear water, cutting into pieces, blanching to remove floating foam, washing with clear water again, and draining to obtain chicken breast pieces for decocting chicken soup; cleaning and cutting the chicken breast B, and mincing the chicken breast B in a meat mincer to obtain chicken paste for preparing chicken enzymatic hydrolysate;
(2) preparing the chicken soup with the added freshness: preparing sodium bicarbonate solution, adding into chicken breast meat pieces, pretreating with ultrasound for 15min, and stewing in nutritional stew pan for 90min to obtain chicken soup crude product;
(3) rotary evaporation and concentration: separating the crude product of chicken soup with 200 mesh filter screen to obtain soup and chicken, and vacuum concentrating the soup at low temperature to obtain semi-finished product of concentrated chicken soup;
(4) preparing chicken enzymatic hydrolysate: adding deionized water into the chicken meat paste, carrying out infrared heating pretreatment, hydrolyzing by using flavourzyme, and then adding neutral protease for hydrolysis to obtain chicken meat enzymolysis liquid;
(5) preparing chicken enzymolysis powder: heating the chicken enzymolysis liquid to 90 ℃, inactivating the neutral protease and the flavor protease for 20 minutes, centrifuging the chicken enzymolysis liquid for 10 minutes, taking supernatant, and freeze-drying into powder to obtain chicken enzymolysis powder;
(6) blending a semi-finished product of the specially fresh concentrated chicken soup: adding the chicken enzymolysis powder, the scallop meat extract and the salt into the semi-finished product of the concentrated chicken soup, blending, and keeping the temperature to obtain a semi-finished product of the specially fresh concentrated chicken soup;
(7) preparing a finished product of the specially fresh concentrated chicken soup: adding flavoring substances into the semi-finished product of the specially fresh concentrated chicken soup, blending, and keeping the temperature to obtain the finished product of the specially fresh concentrated chicken soup.
2. The method for preparing the concentrated soup base combining boiling concentration and compound enzymolysis as claimed in claim 1, wherein the fresh chicken breast meat A in step (1) is cut into chicken breast meat pieces with the specification of 2cm x2cm, and is blanched for 2min to remove froth; the time for beating the chicken meat paste by the meat grinder is 1 min.
3. The method for preparing concentrated soup base by combining decocting concentration and compound enzymolysis according to claim 1, wherein the step (2) comprises the steps of adding the mixture of 0.1% baking soda solution and 1: 2, 50g of chicken breast pieces are added, and the ultrasonic treatment condition is 45KHZ and 120W.
4. The method for preparing concentrated soup base combining decocting concentration and composite enzymolysis according to claim 1, wherein the conditions of the soup in the step (3) of vacuum low-temperature concentration are-0.09 MPa to-0.1 MPa, and the concentrated soup base is subjected to rotary evaporation concentration at 55 ℃ until the solid content is 20% -30%.
5. The preparation method of the concentrated soup base combining decocting concentration and composite enzymolysis as claimed in claim 1, wherein the deionized water added to the chicken meat emulsion in the step (4) is 1: 2.5, w: w, the substrate concentration is 28.57 percent, the infrared heating treatment is carried out for 30min at the temperature of 35 ℃, after the treatment, flavourzyme with the weight of 0.1 percent of that of chicken is used for hydrolysis for 1.5h in water bath at the temperature of 54 ℃, and then neutral proteinase with the weight of 0.25 percent of that of the chicken is added for hydrolysis for 2.5 h in water bath at the temperature of 54 ℃.
6. The preparation method of the concentrated soup base combining decocting concentration and compound enzymolysis as claimed in claim 1, wherein the chicken enzymolysis liquid is centrifuged in a 50mL centrifuge tube at 3922g relative centrifugal force at 4 ℃ for 10 minutes, and the supernatant is collected and lyophilized into powder in step (5).
7. The preparation method of the concentrated soup base combining boiling concentration and compound enzymolysis as claimed in claim 1, wherein 4-6% of edible salt, 0.5-2% of scallop meat and 2% of chicken meat enzymolysis powder are added according to the weight percentage of the semi-finished product of the concentrated chicken soup in the step (6); the blending temperature is 81-85 ℃, and the heat preservation time is 30-50 minutes.
8. The preparation method of the concentrated soup base combining decocting concentration and compound enzymolysis as claimed in claim 1, wherein the flavoring substance in step (7) comprises the following raw materials by weight percent: 5% gelatin, 2% sucrose; the blending temperature is 81-85 ℃, and the heat preservation time is 30-50 minutes.
CN202210025015.8A 2022-01-10 2022-01-10 A kind of concentrated soup base preparation method combining boiling concentration and compound enzymolysis Pending CN114403407A (en)

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